CN105638752B - A kind of organic peach vegetalitas peach smaller green leaf hopper insecticide and its application method - Google Patents

A kind of organic peach vegetalitas peach smaller green leaf hopper insecticide and its application method Download PDF

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CN105638752B
CN105638752B CN201610110302.3A CN201610110302A CN105638752B CN 105638752 B CN105638752 B CN 105638752B CN 201610110302 A CN201610110302 A CN 201610110302A CN 105638752 B CN105638752 B CN 105638752B
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fresh
leaf
peach
pieris
human urine
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CN105638752A (en
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杨洁
易娇
郭兆武
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Changsha University of Science and Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/16Ericaceae [Heath or Blueberry family], e.g. rhododendron, arbutus, pieris, cranberry or bilberry

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  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
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  • Agronomy & Crop Science (AREA)
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  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of organic peach vegetalitas peach smaller green leaf hopper insecticide and its application method, described botanical pesticide is by the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf compatibility human urine of tripterygium wilfordii is brewed forms, and the fresh stem apex of its Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5~2.5:0.5~1.5:2.0~3.0:4.0~7.0, it is preferably in a proportion of 2.0:1.0:2.5:5.5, four components mass ratio by weight calculates, and wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, has synergistic function between ingredients.This brewed dose of killing effect to organic peach smaller green leaf hopper is notable.40~60kg.667m is pressed in this agent‑2Dosage 1.0:2.0 are watered dilution spraying.This brewed dose of stable dosage forms, raw material sources enrich, easy to use in production, simple to operate, and cost is low, without any chemical contamination, comply fully with the requirement of organic peach production.

Description

A kind of organic peach vegetalitas peach smaller green leaf hopper insecticide and its application method
Technical field
The invention belongs to the practical new technology research and development of agricultural and application, the present invention relates to a kind of organic small greenery of peach vegetalitas peach The research and development of cicada insecticide and production application method.
Background technology
Peach(Amygdalus persicaL.)There are the mutation such as nectarine, peento, flowering peach, Prunus persica f. densa, ornamental peaches and kind, belong to Defoliation small arbor, high 3-8m;Tree crown is broad and open and flat;Bark chocolate, coarse when old is in flakey;Sprig is elongated, hairless, Glossy, green, area without shade is transformed into red, has a large amount of small hole skins;Hibernaculum cone, top is blunt, outer by pubescence, normal 2-3 Individual fasciation, centre is leaf bud, and both sides are bud.The oval lanceolar of blade, elliptic-lanceolate or ovum shape lanceolar, long 7- 15cm, wide 2-3.5cm, tip is tapering, base portion wide wedge shape, above it is hairless, have a small number of pubescence or hairless, leaf between arteries and veins below Side has serration or rough sawn tooth, increment tool body of gland or Non-gland body;Petiole is sturdy, long 1-2cm, often with 1 to several pieces bodies of gland, sometimes without Body of gland.Flower Dan Sheng, is opened, diameter 2.5-3.5cm prior to leaf;Bennet is extremely short or several no stalks;Calyx tube is bell, dilute several by pubescence It is hairless, green and have punctation;Sepal is avette to Long Circle, and nose-circle is blunt, outer by pubescence;The long round shape ellipse of petal is extremely Wide obovate, pink are rare for white;Stamen about 20-30, flower pesticide blush;Style is several isometric or slightly shorter with stamen;Ovary quilt Pubescence.Fruit shapes and size have variation, avette, wide ellipse or oblate, diameter 5-7cm, long several equal with width, color Pool change is by pale green white to orange-yellow, and often in mask blush on the sunny side, outside is close by pubescence, and dilute hairless, abdomen seam is obvious, carpopodium It is short and go deep into fruit low-lying area;Pulp white, light green white, yellow, orange-yellow or red, succulence is savory, sweet tea or sour-sweet;Core is big, from Core or clingstone, ellipse or subcircular, both sides are flat, and top is tapering, and surface has longitudinal and transverse rill and hole;Benevolence bitter, dilute taste Sweet tea.The month at florescence 3-4, fructescence is different because of kind, usually the 8-9 months.Peach originates in China, and China cultivates each provinces and regions extensively, Also there is plant all over the world.There is Bei Jinghai in main economic cultivation area in North China, East China each province, the area more concentrated Shallow lake area, Pinggu county, Jixian Tianjin, Mengyin, Shandong, Feicheng, Yidu, Qingdao, Henan Shangshui, Kaifeng, Funing, Hebei province, Zunhua, depth County, Linzhang, Baoji, Xi'an, Tianshui city, Sichuan Chengdu, Dalian, Zhejiang Fenghua, Shanghai Nanhui, jiangsu wuxi, The ground such as Xuzhou.Peach is that a kind of raw economy of drought of high-yield and high-efficiency and ornamental plant, various regions have extensive cultivation, with plantation real estate sector Structural adjustment, its cultivated area have the gesture of continuous expansion.In recent years, peach pest and disease damage was on the rise, it has also become development peach One big obstacle of production.In recent years, the harm of peach smaller green leaf hopper is more universal, it has also become one of Major Pests of peach.Peach smaller green leaf hopper Also known as some leafhopper, peach leafhopper, 4-6 generations occur within 1 year, got over adult in the evergreen tree near fallen leaves, weeds or peach garden In the winter, between the month in next year 3-4 during peach rudiment, migrate and inhale harm to thorn on peach tender leaf, yellow-white dot initially occur on killed leaf, sternly Spot is connected during weight, one-piece blade is changed into pale asphyxia, untimely defoliation.It is the first generation at the beginning of 6 months, until September forth generation, October seeks Survived the winter in green thick grass, winter crop, evergreen tree silk seam.
Mainly have to the prevention and controls of peach insect pest at present:1. cultural control.Winter removes dry branches and fallen leaves, weeds, scrapes in time Except skin is stuck up, concentration is burnt, and reduces insect hibernacle.2. biological control.At present there is no ectogenic natural enemy.3. change Learn preventing and treating.Spray and prevent and treat in following three critical periods:Thank and spend rear young sprout leaf stretching period, late May first generation nymph hatching peak period, Second generation nymph incubation period late July to early August, can with 5% effective cypermethrin, 2000 times of liquid, go out and sweep 3000 times of liquid of profit etc. Chemical pesticide is sprayed.
Prevention and controls above are all effective, and some even have remarkable result, such as chemical prevention.But these preventing and treating sides Method also has the shortcomings that its is obvious, such as:Cultural control is time consuming, and in the case where current rural area green grass or young crops strong labour power is few, problem is more prominent Go out;At present there is no ectogenic natural enemy for biological control;Although chemical prevention works well, pollution residual is big, is not inconsistent Close organic production requirement.And this organic peach vegetalitas peach smaller green leaf hopper insecticide overcomes the above shortcoming, this organic peach is planted Physical property peach smaller green leaf hopper insecticide raw material is taken from free of contamination natural growing plants, and its natural decomposition is fast, and the residence time is short, Easy to use, simple to operate, cost is low, and insecticidal effect is notable, without any chemical contamination, complies fully with wanting for the organic peach of production Ask.
The content of the invention
The purpose of the present invention aims to provide a kind of organic peach vegetalitas peach smaller green leaf hopper insecticide, this insecticide user Just, simple to operate, cost is low, and insecticidal effect is notable.It is small that another object of the present invention aims to provide above-mentioned organic peach vegetalitas peach The preferable application method of greenery cicada insecticide.The purpose of the present invention is achieved in the following ways:
The insecticide of the present invention is the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine is brewed forms, wherein The fresh cauline leaf of tripterygium wilfordii is mainly used as auxiliary agent, plays a part of to promote its insecticidal effect;Human urine is used as brewed solvent.Inventor studies hair Existing, good synergistic function can be played by adding the fresh cauline leaf of auxiliary agent tripterygium wilfordii.Need it is emphasized that this organic peach vegetalitas The fresh stem apex of Coriaria sinica of peach smaller green leaf hopper insecticide, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected from soil, water, sky The free of contamination area of the environmental conditions such as gas, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii grows naturally to be formed, not Used the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.This organic peach vegetalitas peach smaller green leaf hopper kills The preparation method of worm agent is:The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected except above-mentioned described Outer from the free of contamination area of the environmental conditions such as soil, water, air, soil, water body and organic fertilizer etc. also want heavy metal free to pollute.System First the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii mass ratio by weight are put into a certain amount of human urine and soaked when standby .The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine four components weight mass ratio be 1.5~2.5: 0.5~1.5:2.0~3.0:4.0~7.0.The fresh stem apex of the component Coriaria sinica of the above four, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine Most preferred weight mass ratio be 2.0:1.0:2.5:5.5.The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine Calculated by fresh weight mass ratio, human urine is calculated by 1~30d weight mass ratio.The fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii are fresh Cauline leaf, the four components mass ratio by weight of human urine mix, 20~40d of the big Plastic Drum capping immersions of 25~50L(Should not during immersion Sealing), with 3~4 layers of filtered through gauze after 20~40d, its filtrate is this organic peach vegetalitas peach smaller green leaf hopper insecticide, plastics Bag hermetic package, 2-3 can be preserved.When killing peach smaller green leaf hopper using this organic peach vegetalitas peach smaller green leaf hopper insecticide, need Ensure that all sites such as leaf, branch bar have been sprayed onto position.Desinsection dosage, typically press 1.0 with filtrate:2.0 are watered dilution spraying, with worm The leaf of strain, branch bar is sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position, peach smaller green leaf hopper can be carried out compared with Good killing.The production of this insecticide is upper easy to use, and simple to operate, cost is low, and insecticidal effect is notable, and the shelf-life is longer, with modeling Pocket packs, and can carry out factorial praluction, forms product.The present invention selects the fresh stem apex of Coriaria sinica, pieris fresh leaf and auxiliary agent The fresh cauline leaf of tripterygium wilfordii, brewed medium human urine, the compatibility of suitable quality is employed, is configured to commercial botanical pesticide, it has Significant insecticidal effect.This organic peach vegetalitas peach smaller green leaf hopper insecticide is easy to use, and simple to operate, cost is low, desinsection effect Fruit is notable, and this is that other physical measures can not be than.It is the comparative test of its insecticidal effect below.
Organic peach vegetalitas peach smaller green leaf hopper insecticide of the present invention has no the report of like product at present, and the present invention is hair The result of the test that a person of good sense is drawn by experimental study for many years with production practices, and tested in the production of long period Card, effect are preferable.And the formula of the present invention is also higher with technology content, formulation is relatively stable, and raw material sources enrich, operation letter Just, it is cheap for producing organic peach as developed into commodity.Easy to use in production, insecticidal effect is notable, Use cost is relatively low, is a kind of botanical pesticide of the good organic peach of production.Organic peach vegetalitas peach smaller green leaf hopper desinsection Agent prescription can carry out factorial praluction, and it is small green that its utilization not only will commercially increase a kind of new organic peach vegetalitas peach Leafhopper insecticide, also a new solution route will be provided the problem of organic peach deinsectization difficulty to produce at present.
The present invention compound method be:First by the fresh stem apex of Coriaria sinica, pieris fresh leaf and the fresh cauline leaf of tripterygium wilfordii during preparation Soil, water are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry.Thereafter, the fresh stem apex of Coriaria sinica, pieris fresh leaf, Thunder God The fresh cauline leaf of rattan, the weight mass ratio of the component of human urine four are 1.5~2.5:0.5~1.5:2.0~3.0:4.0~7.0, the fresh stem of Coriaria sinica Point, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and the big Plastic Drum capping immersions 20 of 25~50L~ 40d(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is that this organic peach vegetalitas peach is small after 20~40d Greenery cicada insecticide, plastic bag sealing packaging, can preserve 2-3.
The present invention application method be:During using this organic peach vegetalitas peach smaller green leaf hopper insecticide desinsection, need to ensure leaf, The all sites such as branch bar have been sprayed onto position.Desinsection dosage, typically press 1.0 with filtrate:2.0 be watered dilution spraying, with the leaf of worm strain, Branch bar, which is sprayed onto, to be advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position, peach smaller green leaf hopper can preferably be killed Go out.Being typically chosen in before at 11 points in the fine day morning or being sprayed after at 15 points in afternoon is advisable, to be ensured after spraying do not rained in 24h or Do not sprayed manually, if having been rained in 24h or having carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is as far as possible fine, with Do not formed on plant and be limited compared with large water drop, safe defence strictly will carry out human body defence and behaviour by chemical pesticide operational procedure Make.Sylvan life can not carry out the mixed battalion of breeding in 30d after spray.Testing inspection shows, uses this organic peach vegetalitas peach smaller green leaf hopper Insecticide, the organic peach of commodity thus produced is detected, the main functional component of its botanical pesticide is not examined Go out.
It is that inventor realizes the purpose of the present invention below, is constantly selected by experiment and had from suitable of the invention The comparative test for the insecticidal effect that the raw material of machine peach vegetalitas peach smaller green leaf hopper insecticidal formulation and its proportioning of correlation are done.
1. single group separating tests
Single group separating tests are mainly that each component of qualitative test is tried the single effect of organic peach smaller green leaf hopper killing action Test.
1.1 materials and methods
1.1.1 test material
Experiment endangers more universal smaller green leaf hopper to the production of organic peach.Experiment is with single dose:The fresh stem apex of Coriaria sinica, horse are liquor-saturated Four the fresh cauline leaf of wooden fresh leaf, tripterygium wilfordii, human urine components.Wherein human urine is brewed medium.
1.1.2 test method
The preparation of test material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected From the free of contamination area of the environmental conditions such as soil, water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii is naturally raw Length forms, and does not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.First by the fresh stem of Coriaria sinica during preparation Point, pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry. Thereafter, the fresh stem apex of Coriaria sinica and the weight mass ratio of human urine are 2.0:5.5, it is capped with the big Plastic Drums of 1~30d 25~50L of human urine Immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging is protected Deposit 3 years.The weight mass ratio of pieris fresh leaf and human urine is 1.0:5.5, added with the 1~30d big Plastic Drums of 25~50L of human urine Lid immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing is packed Preserve 3 years.The fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 2.5:5.5, with the 1~30d big Plastic Drums of 25~50L of human urine Capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing bag Dress preserves 3 years.Human urine, with 1~30d human urine, the big Plastic Drum capping storage 30d of 25~50L(It not sealed during storage). With 3 layers of filtered through gauze after 30d, its filtrate is obtained.Plastic bag sealing packaging preserves 3 years.Desinsection dosage, 1.0 are pressed with filtrate:2.0 Dilution spraying is watered, with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position.Selection Sprayed before at 11 points in the fine day morning or after at 15 points in afternoon, ensure after spraying and do not rained in 24h, during which also without artificial mark spraying Fill.Contrast is sprayed with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid, viewing test effect after 24h, is ensured after spraying in 24h Do not rain, during which also without artificial sprinkling irrigation.Test effect mainly with the percentage of its smaller green leaf hopper death rate come count with Statistical analysis.Spraying time is carried out before smaller green leaf hopper is broken out.
Test in orchard:Experiment sets 5 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random alignment.Each processing numbering is processing 1-5 and CK1.
Laboratory test:Experiment sets 5 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (Referring to net has the basin of smaller green leaf hopper and peach fresh-cut branches and leaves), respectively handle random alignment.Each processing numbering is processing 6-10 and CK2.
Each disposition is as follows:
Processing 1 --- the fresh stem apex of Coriaria sinica and the weight mass ratio of human urine are 2.0::5.5, with 1~30d 25~50L of human urine Big Plastic Drum capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastics Bag hermetic package preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Examination is observed after 24h Test effect.Processing 2 --- the weight mass ratio of pieris fresh leaf and human urine is 1.0:5.5, it is big with 1~30d 25~50L of human urine Plastic Drum capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag Pack preservation 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test after 24h Effect.Processing 3 --- the fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 2.5:5.5, it is big with 1~30d 25~50L of human urine Plastic Drum capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag Pack preservation 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test after 24h Effect.Processing 4 --- with 1~30d human urine, the big Plastic Drum capping storage 30d of 25~50L(It not sealed during storage).30d Afterwards with 3 layers of filtered through gauze, its filtrate is obtained.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this urine-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test effect after 24h.Processing 5 --- with 2000 times of 5% effective cypermethrin of chemical pesticide Liquid 50kg.667m-2Dosage spray, viewing test effect after 24h.Control(CK1)--- water spray 50kg.667m-2Use Measure, viewing test effect after 24h.Processing 6 --- the fresh stem apex of Coriaria sinica and the weight mass ratio of human urine are 2.0::5.5, with 1~30d 25~50L of human urine big Plastic Drums capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain Obtain its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spray Mist.Viewing test effect after 24h.Processing 7 --- the weight mass ratio of pieris fresh leaf and human urine is 1.0:5.5, with 1~30d 25~50L of human urine big Plastic Drums capping immersion immersion 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain Obtain its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spray Mist.Viewing test effect after 24h.Processing 8 --- the fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 2.5:5.5, with 1~ The 30d big Plastic Drum capping immersion immersion 30d of 25~50L of human urine(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, Obtain its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spray Mist.Viewing test effect after 24h.Processing 9 --- with 1~30d human urine, the big Plastic Drum capping storage 30d of 25~50L(Storage Period not seal).With 3 layers of filtered through gauze after 30d, its filtrate is obtained.Plastic bag sealing packaging preserves 3 years.Pressed with this urine 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test effect after 24h.Processing 10 --- with chemical pesticide 5% 2000 times of liquid 50kg.667m of effective cypermethrin-2Dosage spray, viewing test effect after 24h.Control(CK2)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.
1.2 statistical analysis
SAS is used to test data(6.12)Carry out statistical analysis.Result of the test shows(Table 1), the processing of orchard experiment 1-3, the 5 peach smaller green leaf hopper death rate are significantly higher than CK1, handle the 4 peach smaller green leaf hopper death rate with CK1 without significant difference, place The peach smaller green leaf hopper death rate of reason 5 is significantly higher than other processing.Described above, the fresh stem apex of Coriaria sinica, the pieris of orchard experiment are fresh The peach smaller green leaf hopper death rate of the fresh cauline leaf of leaf, tripterygium wilfordii is all significantly higher than human urine and CK1, but substantially less than chemical pesticide 5% is efficient The processing 5 of cypermethrin, human urine processing and CK1 the peach smaller green leaf hopper death rate are without significant difference.
The single group separating tests of table 1. --- the death rate of peach smaller green leaf hopper(Unit:%)
Processing The death rate Processing The death rate
Processing 1 28.41±4.62bc Processing 6 24.90±1.50c
Processing 2 31.95±1.79b Processing 7 28.62±3.41b
Processing 3 26.61±0.64c Processing 8 26.39±0.73bc
Processing 4 2.36±1.28d Processing 9 0.91±0.36d
Processing 5 96.01±6.91a Processing 10 97.68±4.09a
CK1 1.83±0.53d CK2 0.73±0.24d
Note:Lowercase is 0.05 horizontal, and the different letters of same row represent significant difference, similarly hereinafter.
The processing 6-8 of laboratory test, the 10 peach smaller green leaf hopper death rate are significantly higher than CK2, handle 9 peach smaller green leaf hopper For the death rate with CK2 without significant difference, the peach smaller green leaf hopper death rate for handling 10 is significantly higher than other processing.It is described above, experiment The fresh stem apex of Coriaria sinica of room experiment, pieris fresh leaf, the peach smaller green leaf hopper death rate of the fresh cauline leaf of tripterygium wilfordii be all significantly higher than human urine with CK2, but the substantially less than processing 10 of the effective cypermethrin of chemical pesticide 5%, human urine processing and the CK2 peach smaller green leaf hopper death rate Without significant difference.
Tested by above one pack system orchard and shown with laboratory test, the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii The death rate of the peach smaller green leaf hopper of fresh cauline leaf is all significantly higher than human urine processing and CK1, CK2, but substantially less than chemical pesticide 5% is high Imitate cypermethrin processing.CK1, CK2 of human urine processing and the water spray death rate are without significant difference.
2. compatibility mechanism
Compatibility mechanism is mainly that qualitative and quantitative tests each ingredients small greenery more universal to killing the organic peach of harm The composite effect experiment of cicada.
2.1 materials and methods
2.1.1 test material
Experiment endangers more universal smaller green leaf hopper to the production of organic peach.Experiment is that the fresh stem apex of Coriaria sinica, horse are liquor-saturated with ingredients Four the fresh cauline leaf of wooden fresh leaf, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
2.1.2 test method
The preparation of test material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected From the free of contamination area of the environmental conditions such as soil, water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii is naturally raw Length forms, and does not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.First by the fresh stem of Coriaria sinica during preparation Point, pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry. Thereafter, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5~2.5:0.5 ~1.5:2.0~3.0:4.0~7.0, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix It is even, 20~40d of the big Plastic Drum capping immersions of 25~50L(It not sealed during immersion), with 3~4 layers of gauze mistake after 20~40d Filter, its filtrate are this organic peach vegetalitas peach smaller green leaf hopper insecticide, and plastic bag sealing is packed, to preserve the vegetalitas of 3 years Insecticide is experiment insecticide stoste.Desinsection dosage, 1.0 are pressed with filtrate:2.0 are watered dilution spraying, with the leaf of worm strain, branch bar Being sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position.Selection is before at 11 points in the fine day morning or at 15 points in afternoon Spray later, ensure after spraying and do not rained in 24h, during which also without artificial sprinkling irrigation.The efficient chlorine cyanogen of chemical pesticide 5% is used in contrast 2000 times of liquid of chrysanthemum ester are sprayed, viewing test effect after 24h, are ensured after spraying and are not rained in 24h, during which also without artificial mark spraying Fill.Test effect is mainly counted and statistical analysis with the percentage of its smaller green leaf hopper death rate.Spraying time is small Carried out before the outburst of greenery cicada.
Test in orchard:Experiment sets 4 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random alignment.Each processing numbering is processing 1-4 and CK1.
Laboratory test:Experiment sets 4 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (Referring to net has the basin of smaller green leaf hopper and peach fresh-cut branches and leaves), respectively handle random alignment.Each processing numbering is processing 5-8 and CK2.
Each disposition is as follows:
Processing 1 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5:0.5:2.0:4.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Seen after 24h Examine test effect.Processing 2 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four For 2.0:1.0:2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 it is watered dilution spraying.After 24h Viewing test effect.Processing 3 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight quality of the component of human urine four Than for 2.5:1.5:3.0:7.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h Viewing test effect afterwards.Processing 4 --- with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage spray Mist, viewing test effect after 24h.Control(CK1)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.Place Reason 5 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5:0.5: 2.0:4.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test is imitated after 24h Fruit.Processing 6 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0: 1.0:2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Examination is observed after 24h Test effect.Processing 7 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.5:1.5:3.0:7.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Seen after 24h Examine test effect.Processing 8 --- with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage spray, 24h Viewing test effect afterwards.Control(CK2)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.
2.2 statistical analysis
SAS is used to test data(6.12)Carry out statistical analysis.Result of the test shows(Table 2), orchard is tested everywhere The peach smaller green leaf hopper death rate of reason is significantly higher than CK1, and the peach smaller green leaf hopper death rate for handling 4 is significantly higher than processing 1-3 and CK1, The peach smaller green leaf hopper death rate of processing 1, which is substantially less than, handles 2-4, handles the 2-3 death rate without significant difference, handles the dead of 3-4 Rate is died without significant difference.It is described above, the fresh stem apex of Coriaria sinica of orchard experiment, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, four groups of human urine The weight mass ratio divided is 1.5~2.5:0.5~1.5:2.0~3.0:4.0~7.0, press 1.0 with this compatibility liquid stoste:2.0 convert Water dilution spraying, the effect of the orchard experiment after 24h is notable to the killing action of peach smaller green leaf hopper, and efficient with chemical pesticide 5% The spraying of 2000 times of liquid of cypermethrin, test effect after 24h substantially close to.Wherein, the fresh stem apex of Coriaria sinica, pieris fresh leaf, Thunder God The fresh cauline leaf of rattan, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:It is fresh that 5.5 orchard test effect is significantly better than Coriaria sinica Stem apex, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5:0.5:2.0:4.0 experiment effect Fruit, but the weight mass ratio of stem apex fresh with Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the component of human urine four is 2.5:1.5: 3.0:7.0 effect is essentially identical.Consider from economic benefit combination insecticidal effect, the fresh stem apex of Coriaria sinica, pieris fresh leaf, thunder The fresh cauline leaf of public rattan, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5 compatibility be kill peach smaller green leaf hopper compared with Good ingredients.
The compatibility mechanism of table 2. --- the death rate of peach smaller green leaf hopper(Unit:%)
Processing The death rate Processing The death rate
Processing 1 81.37±1.82c Processing 5 79.62±0.67c
Processing 2 87.61±0.64b Processing 6 86.49±2.40b
Processing 3 89.25±2.41ab Processing 7 87.20±1.15b
Processing 4 93.17±3.70a Processing 8 95.26±4.31a
CK1 0.93±0.42d CK2 1.20±0.49d
The peach smaller green leaf hopper death rate of each processing of laboratory test is significantly higher than CK2, handles 8 peach smaller green leaf hopper death Rate is significantly higher than processing 5-7 and CK2, and the peach smaller green leaf hopper death rate for handling 5 is substantially less than processing 6-8, handles the 6-7 death rate Without significant difference.It is described above, the fresh stem apex of Coriaria sinica of laboratory test, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the component of human urine four Weight mass ratio be 1.5~2.5:0.5~1.5:2.0~3.0:4.0~7.0, press 1.0 with this compatibility liquid stoste:2.0 it is watered Dilution spraying, the effect of the laboratory test after 24h is notable to the killing action of peach smaller green leaf hopper, and efficient with chemical pesticide 5% The spraying of 2000 times of liquid of cypermethrin, test effect after 24h substantially close to.Wherein, the fresh stem apex of Coriaria sinica, pieris fresh leaf, Thunder God The fresh cauline leaf of rattan, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5 laboratory test significant effect is better than Coriaria sinica Fresh stem apex, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 1.5:0.5:2.0:4.0 experiment Effect, but the weight mass ratio of stem apex fresh with Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the component of human urine four is 2.5:1.5: 3.0:7.0 effect is essentially identical.Consider from economic benefit combination insecticidal effect, the fresh stem apex of Coriaria sinica, pieris fresh leaf, thunder The fresh cauline leaf of public rattan, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5 compatibility be kill peach smaller green leaf hopper compared with Good ingredients.
Experiment is it is also shown that the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the experiment of the component compatibility of human urine four above Peach smaller green leaf hopper the death rate(Table 2)It is significantly higher than the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, four groups of human urine Divide the death rate of single dose experiment peach smaller green leaf hopper(Table 1).It is indicated above that the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh stem of tripterygium wilfordii Leaf, the component compatibility of human urine four have preferable synergistic function.
3. dosetest
Dosetest is mainly that qualitative and quantitative tests each dosage and endangers more universal small of organic peach to killing The effect test of greenery cicada.
3.1.1 test material
Experiment endangers more serious smaller green leaf hopper to the production of organic peach.Experiment is that the fresh stem apex of Coriaria sinica, horse are liquor-saturated with ingredients Four the fresh cauline leaf of wooden fresh leaf, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
3.1.2 test method
The preparation of test material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected From the free of contamination area of the environmental conditions such as soil, water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii is naturally raw Length forms, and does not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.First by the fresh stem of Coriaria sinica during preparation Point, pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry. Thereafter, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and 25~50L is big, and Plastic Drum adds 20~40d of lid immersion(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is this organic peach after 20~40d Vegetalitas peach smaller green leaf hopper insecticide, plastic bag sealing packaging, to preserve the botanical pesticide of 3 years as experiment raw insecticide Liquid.Desinsection dosage, 1.0 are pressed with filtrate:2.0 are watered dilution spraying, and with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure leaf, branch The all sites such as bar have been sprayed onto position.Selection is sprayed before at 11 points in the fine day morning or after at 15 points in afternoon, and 24h is ensured after spraying Do not rain inside, during which also without artificial sprinkling irrigation.Contrast is sprayed with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid, after 24h Viewing test effect, ensure after spraying and do not rained in 24h, during which also without artificial sprinkling irrigation.Test effect is mainly small with its The percentage of the greenery cicada death rate counts and statistical analysis.Spraying time is carried out before smaller green leaf hopper is broken out.
Test in orchard:Experiment sets 4 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random alignment.Each processing numbering is processing 1-4 and CK1.
Laboratory test:Experiment sets 4 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (Referring to net has the basin of smaller green leaf hopper and peach fresh-cut branches and leaves), respectively handle random alignment.Each processing numbering is processing 5-8 and CK2.
Each disposition is as follows:
Processing 1 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Seen after 24h Examine test effect.Processing 2 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four For 2.0:1.0:2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After 24h Viewing test effect.Processing 3 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight quality of the component of human urine four Than for 2.0:1.0:2.5:5.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h Viewing test effect afterwards.Processing 4 --- press 50kg.667m with chemical pesticide 5% effective cypermethrin, 2000 times of liquid-2Dosage spray Mist, viewing test effect after 24h.Control(CK1)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.Place Reason 5 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0: 2.5:5.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test is imitated after 24h Fruit.Processing 6 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0: 1.0:2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Examination is observed after 24h Test effect.Processing 7 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Seen after 24h Examine test effect.Processing 8 --- press 50kg.667m with chemical pesticide 5% effective cypermethrin, 2000 times of liquid-2Dosage spray, Viewing test effect after 24h.Control(CK2)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.
3.2 statistical analysis
SAS is used to test data(6.12)Carry out statistical analysis.Result of the test shows(Table 3), the processing of orchard experiment The 1-4 peach smaller green leaf hopper death rate is all remarkably higher than CK1.Processing 2-4 the peach smaller green leaf hopper death rate be significantly higher than processing 1 with Without significant difference between CK1, processing 2-3;Processing 2 is significantly higher than processing 1.It is described above, the fresh stem apex of Coriaria sinica of orchard experiment, horse Liquor-saturated wooden fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5, with this compatibility liquid stoste By 40-60kg.667m-2Dosage 1.0:2.0, which are watered dilution, sprays, and the orchard test effect after 24h kills to peach smaller green leaf hopper The effect of going out is notable, and with being sprayed with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid, the test effect observed after 24h connects substantially Closely.Wherein, compatibility liquid stoste presses 50kg.667m-2The orchard test effect of dosage be significantly better than compatibility liquid stoste by 40kg.667m-2The test effect of dosage, but press 60kg.667m with compatibility liquid stoste-2The test effect of dosage is without significant difference. Consider from economic benefit combination insecticidal effect, compatibility liquid stoste presses 50kg.667m-2Dosage be kill peach smaller green leaf hopper compared with Good dosage.
The dosetest of table 3. --- the death rate of peach smaller green leaf hopper(Unit:%)
Processing The death rate Processing The death rate
Processing 1 83.19±2.07c Processing 5 84.43±1.64c
Processing 2 87.61±1.19b Processing 6 86.49±0.59bc
Processing 3 89.67±3.81ab Processing 7 88.73±2.27b
Processing 4 93.17±4.28a Processing 8 95.26±5.82a
CK1 0.93±0.37d CK2 1.20±0.40d
The processing 5-8 of the laboratory test peach smaller green leaf hopper death rate is all remarkably higher than CK2.Handle the 6-7 small greenery of peach The cicada death rate, which is significantly higher than, handles 5 and CK2, without significant difference between processing 6-7;Processing 6 is substantially better than processing 5.It is described above, The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four of laboratory test are 2.0: 1.0:2.5:5.5, press 40-60kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0, which are watered dilution, sprays, the reality after 24h The killing action that room test effect is tested to peach smaller green leaf hopper is notable, with being sprayed with 5% effective cypermethrin of chemical pesticide, 2000 times of liquid Mist, the test effect observed after 24h substantially close to.Wherein, compatibility liquid stoste presses 50kg.667m-2The laboratory test effect of dosage Fruit is substantially better than compatibility liquid stoste by 40kg.667m-2The test effect of dosage, but press 60kg.667m with compatibility liquid stoste-2Agent The test effect of amount is without significant difference.Consider from economic benefit combination insecticidal effect, compatibility liquid stoste presses 50kg.667m-2Agent Amount is to kill the preferable dosage of peach smaller green leaf hopper.
Experiment is it is also shown that the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the component compatibility dosage of human urine four above The insecticidal effect of experiment is the death rate of peach smaller green leaf hopper(Table 2-3)It is significantly higher than the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii Fresh cauline leaf, the death rate of the component single dose of human urine four experiment(Table 1).It is indicated above that the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii Fresh cauline leaf, the component compatibility of human urine four have preferable synergistic function.
4. auxiliary agent is tested
Auxiliary agent experiment is mainly the synergistic function that qualitative and quantitative experiment auxiliary agent kills smaller green leaf hopper to whole ingredients.
4.1 materials and methods
4.1.1 test material
Experiment endangers more universal smaller green leaf hopper to the production of organic peach.Experiment is that the fresh stem apex of Coriaria sinica, horse are liquor-saturated with ingredients Four the fresh cauline leaf of wooden fresh leaf, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
4.1.2 test method
The preparation of test material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should be selected From the free of contamination area of the environmental conditions such as soil, water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii is naturally raw Length forms, and does not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.First by the fresh stem of Coriaria sinica during preparation Point, pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry. Thereafter, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and 25~50L is big, and Plastic Drum adds 20~40d of lid immersion(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is this organic peach after 20~40d Vegetalitas peach smaller green leaf hopper insecticide, plastic bag sealing packaging, to preserve the botanical pesticide of 3 years as experiment raw insecticide Liquid.Desinsection dosage, with filtrate 50kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection before at 11 points in the fine day morning or under 15 points of noon sprays later, ensures after spraying and is not rained in 24h, during which also without artificial sprinkling irrigation.Contrast is high with chemical pesticide 5% Imitate 2000 times of liquid of cypermethrin to spray, viewing test effect after 24h, ensure after spraying and do not rained in 24h, during which also without people Work is sprayed.Test effect is mainly counted and statistical analysis with the percentage of its smaller green leaf hopper death rate.Spraying time Carried out before smaller green leaf hopper is broken out.
Test in orchard:Experiment sets 3 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random alignment.Each processing numbering is processing 1-3 and CK1.
Laboratory test:Experiment sets 3 processing, 1 control, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (Referring to net has the basin of smaller green leaf hopper and peach fresh-cut branches and leaves), respectively handle random alignment.Each processing numbering is processing 4-6 and CK2.
Each disposition is as follows:
Processing 1 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Seen after 24h Examine test effect.Processing 2 --- the fresh stem apex of Coriaria sinica, pieris fresh leaf, the weight mass ratio of the component of human urine three are 2.0:1.0: 5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test effect after 24h.Place Reason 3 --- press 50kg.667m with chemical pesticide 5% effective cypermethrin, 2000 times of liquid-2Dosage spray, viewing test after 24h Effect.Control(CK1)--- water spray 50kg.667m-2Dosage, viewing test effect after 24h.Processing 4 --- the fresh stem of Coriaria sinica Point, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5.With this compatibility liquid Stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test effect after 24h.Processing 5 --- Coriaria sinica is fresh Stem apex, pieris fresh leaf, the weight mass ratio of the component of human urine three are 2.0:1.0: 5.5.Pressed with this compatibility liquid stoste 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Viewing test effect after 24h.Processing 6 --- with chemical pesticide 5% 2000 times of liquid of effective cypermethrin press 50kg.667m-2Dosage spray, viewing test effect after 24h.Control(CK2)--- spray Water 50kg.667m-2Dosage, viewing test effect after 24h.
4.2 statistical analysis
SAS is used to test data(6.12)Carry out statistical analysis.Result of the test shows(Table 4), the processing of orchard experiment The 1-3 peach smaller green leaf hopper death rate is significantly higher than CK1, and the peach smaller green leaf hopper death rate for handling 2 is substantially less than processing 1,3, but aobvious Work is higher than CK1.Described above, the fresh stem apex of compatibility Coriaria sinica, pieris fresh leaf, the weight mass ratio of the component of human urine three of no auxiliary agent are 2.0:1.0:5.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0, which are watered dilution, sprays, the orchard after 24h The effect of experiment is substantially less than the fresh stem apex of compatibility Coriaria sinica, pieris fresh leaf, the Thunder God that have auxiliary agent to the death rate of peach smaller green leaf hopper The fresh cauline leaf of rattan, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5, press 50kg.667m with this compatibility liquid stoste-2 Dosage 1.0:2.0, which are watered dilution, sprays, the death rate that orchard is tested after 24h;Also significantly lower than use 5% efficient chlorine of chemical pesticide 2000 times of liquid 50kg.667m of Cyano chrysanthemate-2Dosage spray 24h after orchard test the death rate.
The auxiliary agent of table 4. is tested --- the death rate of peach smaller green leaf hopper(Unit:%)
Processing The death rate Processing The death rate
Processing 1 87.61±2.71b Processing 4 86.49±0.74b
Processing 2 58.30±4.83c Processing 5 67.25±5.68c
Processing 3 93.17±2.09a Processing 6 95.26±1.19a
CK1 0.93±0.27d CK2 1.20±0.40d
The processing 4-6 of the laboratory test peach smaller green leaf hopper death rate is significantly higher than CK2, handle 5 peach smaller green leaf hopper it is dead Die rate and be substantially less than processing 4,6, but be significantly higher than CK2.It is described above, the fresh stem apex of compatibility Coriaria sinica of no auxiliary agent, pieris fresh leaf, The weight mass ratio of the component of human urine three is 2.0:1.0:5.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 It is watered dilution to spray, the effect of the laboratory test after 24h is substantially less than the compatibility for having auxiliary agent to the death rate of peach smaller green leaf hopper The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5:5.5, use This compatibility liquid stoste presses 50kg.667m-2Dosage 1.0:2.0, which are watered dilution, sprays, the death rate of laboratory test after 24h; Substantially less than use 5% effective cypermethrin of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage spray 24h after laboratory test The death rate.
Experiment shows above(Table 4), the difference on effect of adding assistant and not adding assistant is notable, and the significant effect of adding assistant is better than The not effect of adding assistant, not its significant effect of adding assistant reduction.It is notable to illustrate that the fresh cauline leaf of auxiliary agent tripterygium wilfordii has in this compatibility Collaboration and synergistic effect.
Experiment also shows(Table 1-4), the effect of ingredients is 1-4 times or so of each single dose effect.So effect after compatibility It is not the addition of simple each single dose effect.Compatibility mechanism is tested to from single dose to test to auxiliary agent again(Table 1-4), the results showed that, The fresh stem apex of compatibility Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5 compatibility liquid stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered the ingredients of dilution spraying, combine and kill from economic benefit It is preferably to select that worm effect, which considers,.Experiment shows, with the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the component of human urine four Compatibility agent spray and do not detect the compositions such as its main component coriarine, mountain laurel toxin, wilfordine in rear 30d commodity peach.
Brewed dose of sheet is stomach poison and action of contace poison, its toxic component coriarine, bay toxin, wilfordine etc. with small green Together the metabolism of its polypide is upset in vivo during leafhopper feeding into it to cause and be poisoned to death, or directly from polypide body surface both sides, stomata is invaded Enter to cause in smaller green leaf hopper body internal metabolic disorder and cause and be poisoned to death, or from joint cell of the polypide without the non-keratinization of wax Gap intrusion causes joint to benumb internal metabolic disorder and finally result in death.
Coriaria sinica(Coriaria nepalensisWall.)Belong to Sapindales, Coriariaceae shrub, its leaf is to life, and papery is extremely Thin keratin, ellipse, raceme are born on biennial branch, petal meat, keel-shaped.Male inflorescence elder generation leaf opens, and spends more It is intensive;Sepal is avette, and edge is translucent, top tool tasselled shape serration;Filigree extends when spending;In the presence of sterile gynoecium.Female inflorescence with Leaf goes out together, aubergine.Berry shape achene, atropurpureus, the month at florescence 3-4, the fruiting period 5-6 months are become by red when ripe.Be distributed in Yunnan, The ground such as Guizhou, Sichuan, Hubei, Hunan, Shaanxi, Gansu.Fruit can carry alcohol.Seed oil expression can paint and ink.Cauline leaf can carry Tannin extract.Complete stool contains coriarine, tutin etc., and its toxic component is coriamyrtin, tutin etc..
Pieris(Piereis polta)Belong to evergreen shrubs or dungarunga, bark sepia, sprig is carried out, hairless;Hibernaculum Obovate, 3-8 pieces of perula, is arranged in imbricate.Leaf is intensive in Zhi Ding;Petiole grows 3~8mm, and the outside of belly has zanjon;Blade leathery, Ellipticity lanceolar, or lanceolar, long 3~8cm, wide 1~2cm, tip is short tapering, and the narrow wedge shape of base portion, edge more than 2/3 has Thin knuckle-tooth, dilute to be bordering on full edge, surface bottle green is glossy, back side light green, and master pulse is raised on two sides, and lateral vein is fallen under the surface, Back side unobvious, scun are netted.Raceme or panicle basidixed or axillary, fasciating in Zhi Ding, long 8~14cm, rachis There is pubescence;Sepal triangular shape is avette, is about 3.5mm;Corolla white, urceolus, top 5 is shallow to be split, sliver subcircular;Stamen 10, flower Silk has long pubescence;Ovary 1, subsphaeroidal, style is elongated, column cap head.Capsule is bordering on oblate spheroid, 3~5mm of diameter, loculicidal, Calyx harbors with style, 4~May of florescence, 7~September of fruiting period.Pieris is distributed widely in the ground such as Zhejiang, Yunnan.Pieris contains two The compound such as the compounds such as terpene, triterpene and flavones, its branches and leaves toxin containing mountain laurel, the mould glycosides of pieris and black laurel poison glycosides, its root Portion's oxykojic acid containing 3-, the methylol -4H- pyrans -4- ketone of 3- hydroxyls -2, phloretin, 3,3 ', 4 ', 7- tetrahydroxy -5- methoxyl groups are yellow The compounds such as ketone, catechol, cupreol.
Tripterygium wilfordii(Tripterygium wilfordii Hook. f.), Celastraceae, tripterygium plant, liana shrub, Main product is in China, Korea, Japan, and China is distributed mainly on various regions and southwest on the south the Yangtze river basin, and main product is in Fujian, Zhejiang The ground such as river, Hubei, Hunan, Anhui, Henan.It is born in in the shade how wet hillside, mountain valley, small stream side bushes.Happiness more cool place Hillside, most preferably grown with the deep sand soil of slant acidity, soil layer or yellow soil.Bitter, pungent, cool in nature, big poison.Thunder God Calamus liana Shrub, high 1~3m, sprig brownish red, there are 4~6 thin ribs, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, Rectangular oval or avette, long 4~7.5cm, wide 3~4cm, tip is suddenly sharp or short tapering, and base portion wealthy wedge shape or circle, edge has Serration, 4~7 pairs of lateral vein, up to leaf margin after it is slightly upper curved;Petiole grows 5~8mm, close by rust hair.Circular cone cyme is narrower, Long 5~7cm, wide 3-4cm, generally there is 3~5 branches.The long round shape of samara, long 1~1.5cm, 1~1.2cm of diameter, seed buttress shaft Shape, up to 10mm.Tripterygium wilfordii is mainly containing wilfordine, celastrus orbiculatus benzyl acid amides, Triptolide etc..
Embodiment
Implementation below and embodiment are intended to further illustrate the present invention, rather than limitation of the present invention.
Embodiment 1:
The preparation of material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should select from soil, The free of contamination area of the environmental conditions such as water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii grow naturally and Into not using the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.During preparation first by the fresh stem apex of Coriaria sinica, Pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry.You Afterwards, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and 25~50L is big, and Plastic Drum adds 20~40d of lid immersion(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is this organic peach after 20~40d Vegetalitas peach smaller green leaf hopper insecticide, plastic bag sealing packaging, can be preserved 2~3 years.Usage time is selected in the small greenery of organic peach Sprayed before cicada outburst, can also carry out early prevention.In use, with filtrate 40kg.667m-2By 1.0:2.0 are watered dilution spraying.Choosing Selected before at 11 points in the fine day morning or sprayed after at 15 points in afternoon, ensure after spraying and do not rained in 24h, during which also without artificial Sprinkling irrigation, if having been rained in 24h or having carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is as far as possible fine, with plant not It is degree to be formed compared with large water drop, and safe defence strictly will carry out human body defence and operation by chemical pesticide operational procedure.30d after spray Interior sylvan life can not carry out the mixed battalion of breeding.
The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0: 2.5:5.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic peach orchard after 24h Insecticidal effect be:The peach smaller green leaf hopper death rate 83.19%, with being pressed with chemical pesticide 5% effective cypermethrin, 2000 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 9.98%, substantially close to the effective cypermethrin of chemical pesticide 5% Insecticidal effect.
Embodiment 2:
The preparation of material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should select from soil, The free of contamination area of the environmental conditions such as water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii grow naturally and Into not using the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.During preparation first by the fresh stem apex of Coriaria sinica, Pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry.You Afterwards, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and 25~50L is big, and Plastic Drum adds 20~40d of lid immersion(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is this organic peach after 20~40d Vegetalitas peach smaller green leaf hopper insecticide, plastic bag sealing packaging, can be preserved 2~3 years.Usage time is selected in the small greenery of organic peach Sprayed before cicada outburst, can also carry out early prevention.In use, with filtrate 50kg.667m-2By 1.0:2.0 are watered dilution spraying.Choosing Selected before at 11 points in the fine day morning or sprayed after at 15 points in afternoon, ensure after spraying and do not rained in 24h, during which also without artificial Sprinkling irrigation, if having been rained in 24h or having carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is as far as possible fine, with plant not It is degree to be formed compared with large water drop, and safe defence strictly will carry out human body defence and operation by chemical pesticide operational procedure.30d after spray Interior sylvan life can not carry out the mixed battalion of breeding.
The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0: 2.5:5.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic peach orchard after 24h Insecticidal effect be:The peach smaller green leaf hopper death rate 87.61%, with being pressed with chemical pesticide 5% effective cypermethrin, 2000 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 5.56%, substantially close to the effective cypermethrin of chemical pesticide 5% Insecticidal effect.
Embodiment 3:
The preparation of material:The fresh stem apex of material Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine all should select from soil, The free of contamination area of the environmental conditions such as water, air, the fresh cauline leaf system of the fresh stem apex of Coriaria sinica, pieris fresh leaf, tripterygium wilfordii grow naturally and Into not using the mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality of organic peach will be influenceed.During preparation first by the fresh stem apex of Coriaria sinica, Pieris fresh leaf and soil, water on the fresh cauline leaf of tripterygium wilfordii are shaked off, and do not need to wash, and the segment of 3-5cm length is cut into after water is dry.You Afterwards, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0:2.5: 5.5, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine mass ratio by weight mix, and 25~50L is big, and Plastic Drum adds 20~40d of lid immersion(It not sealed during immersion), with 3~4 layers of filtered through gauze, its filtrate it is this organic peach after 20~40d Vegetalitas peach smaller green leaf hopper insecticide, plastic bag sealing packaging, can be preserved 2~3 years.Usage time is selected in the small greenery of organic peach Sprayed before cicada outburst, can also carry out early prevention.In use, with filtrate 60kg.667m-2By 1.0:2.0 it is watered dilution spraying.Choosing Selected before at 11 points in the fine day morning or sprayed after at 15 points in afternoon, ensure after spraying and do not rained in 24h, during which also without artificial Sprinkling irrigation, if having been rained in 24h or having carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is as far as possible fine, with plant not It is degree to be formed compared with large water drop, and safe defence strictly will carry out human body defence and operation by chemical pesticide operational procedure.30d after spray Interior sylvan life can not carry out the mixed battalion of breeding.
The fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the weight mass ratio of the component of human urine four are 2.0:1.0: 2.5:5.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic peach orchard after 24h Insecticidal effect be:The peach smaller green leaf hopper death rate 89.67%, with being pressed with chemical pesticide 5% effective cypermethrin, 2000 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 3.50%, substantially close to the effective cypermethrin of chemical pesticide 5% Insecticidal effect.

Claims (3)

  1. A kind of 1. organic peach vegetalitas peach smaller green leaf hopper insecticide, it is characterised in that organic peach vegetalitas peach smaller green leaf hopper Insecticide is by the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, human urine compatibility is brewed forms, wherein the fresh stem apex of Coriaria sinica, horse Liquor-saturated wooden fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the compatibility mass ratio of human urine are 1.5~2.5:0.5~1.5:2.0~3.0:4.0~ 7.0, the fresh stem apex of Coriaria sinica, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii press the weight Mass Calculation of fresh material, and human urine presses 1~30d weight Measure Mass Calculation.
  2. A kind of 2. organic peach vegetalitas peach smaller green leaf hopper insecticide according to claim 1, it is characterised in that the fresh stem of Coriaria sinica Point, pieris fresh leaf, the fresh cauline leaf of tripterygium wilfordii, the compatibility mass ratio of human urine are 2.0:1.0:2.5:5.5.
  3. 3. a kind of organic peach vegetalitas peach smaller green leaf hopper insecticide as claimed in claim 1, it is characterised in that in use, choosing Select and sprayed before peach smaller green leaf hopper population number does not have great outburst, by 40~60kg.667m-2Dosage 1.0:2.0 be watered it is dilute Spraying is released, droplet is as far as possible fine, is limited not formed on plant compared with large water drop, selects before at 11 points in the fine day morning or afternoon Sprayed after 15 points, to be ensured after spraying in 24h and not rain or do not sprayed manually, if having rained or having carried out in 24h Artificial sprinkling irrigation, need to be sprayed again.
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