CN106035412B - If a kind of organic loquat vegetalitas first mite insecticide and its application method - Google Patents

If a kind of organic loquat vegetalitas first mite insecticide and its application method Download PDF

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Publication number
CN106035412B
CN106035412B CN201610428389.9A CN201610428389A CN106035412B CN 106035412 B CN106035412 B CN 106035412B CN 201610428389 A CN201610428389 A CN 201610428389A CN 106035412 B CN106035412 B CN 106035412B
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fresh
mite
green pepper
human urine
tripterygium wilfordii
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CN106035412A (en
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郭兆武
邵亚楠
杨洁
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Changsha University of Science and Technology
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Changsha University of Science and Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/38Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

If the invention discloses a kind of organic loquat vegetalitas first mite insecticide and its application methods, the botanical pesticide is by fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb compatibility human urine of tripterygium wilfordii is brewed forms, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.0~2.0:0.5~1.5:1.5~2.5:3.0~6.0, it is preferably in a proportion of 1.5:1.0:2.0:4.5, four components mass ratio by weight calculates, and wherein the fresh herb of tripterygium wilfordii is auxiliary agent, has synergistic function between ingredients.If this brewed dose notable to the killing effect of organic loquat first mite.40~60kg.667m is pressed in this agent‑2Dosage 1.0:2.0 are watered dilution spraying.This brewed dose of stable dosage forms, raw material sources enrich, easy to use in production, easy to operate, at low cost, without any chemical contamination, comply fully with the requirement of organic Loquat growth.

Description

If a kind of organic loquat vegetalitas first mite insecticide and its application method
Technical field
The invention belongs to agriculture practical new technology research and development and application, if the present invention relates to a kind of organic loquat vegetalitas first mites The research and development of insecticide and production application method.
Background technology
LoquatEriobotrya japonica(Thunb.) Lindl ] it is the rose family, Eriobotrya, originate in China The southeast is gained the name due to leaf-shaped is like Chinese lute musical instrument, and flower can be used as medicine.Loquat is evergreen dungarunga, sets high 3-5m, and highest can Up to 10m or so;Blade is big and grows, thick and have fine hair, and in oblong, shape such as Chinese lute, keratin, lanceolar falls lanceolar, the ovum that falls Shape or oval oblong, long 12-30cm, wide 3-9cm, the anxious point or tapering of apex, base portion is wedge-shaped or gradually narrow into petiole, top edge Have a thin sawtooth, base portion full edge, above it is bright, it is wrinkle more, below dense taupe brown villus, 11-21 pairs of lateral vein;Petiole it is short or there are few Handle, long 6-10mm have taupe brown villus;Stipule bores shape, long 1-1.5cm, the anxious point of apex, hairiness.Sprig is sturdy, yellowish-brown, close Get rusty color or taupe brown villus.Panicle basidixed, long 10-19cm, tool are spent more;Total bennet and the dense rust villus of bennet, flower Obstruct long 2-8mm;Bract bores shape, long 2-5mm, dense rust villus;Flower diameter 12-20mm;Calyx tube tazza shape, long 4-5mm, sepal There are rust villus in triangular egg, long 2-3mm, the anxious point of apex, calyx tube and sepal outside;Petal white, oblong or avette, long 5- 9mm, wide 4-6mm, base portion tool pawl, there is rust villus;Stamen 20, considerably shorter than petal, the extension of filigree base portion;Style 5, from life, column Head's shape, hairless, there is rust pubescence on ovary top, and there are 2 ovules in Room 5 per room.Fruit is spherical or oblong, diameter 2-5cm are yellow Color or orange colour have rust pubescence, come off soon outside;Seed 1-5, spherical or oblate spheroid, diameter 1-1.5cm, brown is bright, Kind mulberry paper matter.The month at florescence 10-12, the fruiting period 5-6 months.Also bunchy is hung on the tree ripe loquat fruit, each long 3-5cm of fruit, Into round, oval or long shape " Chinese lute shape ".Loquat surface is by villous, and dark green when underdone, stronger, aromatic odor is denseer. Crust is generally faint yellow after maturation, also there is that color is deeper, close to orange-red.Pulp is soft and succulence, can be divided mainly into white And orange two kinds.Different from most of fruit tree, loquat is in autumn or early winter blooms, and fruit is ripe in spring to early summer, than other Fruit is early, therefore is " solely standby seasonal pathogens,exopathogens person in fruit tree " by title.Fruit soft and succulency, sweet and sour palatability is full of nutrition, leaf, flower, Fruit, benevolence can control cough.The tough and tensile exquisiteness of timber is engraving, makes the high-quality timber of top-grade furniture.Loquat is mainly distributed on sweet Respectful, Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei, Hunan, Sichuan, Yunnan, Guizhou, Guangxi, Guangdong, Fujian, Taiwan Etc. ground.The wide row cultivation in various regions, there are wild species in Sichuan, Hubei.Also there are cultivation in Japan, India, Vietnam, Burma, Thailand, Indonesia Training.
Loquat is a kind of raw economic plants of drought of high-yield and high-efficiency, and there are extensive cultivation in various regions, with the planting site industrial structure Adjustment, cultivated area have the gesture of continuous expansion.In recent years, loquat pest and disease damage was on the rise, it has also become development loquat One big obstacle of fruit industry production.If loquat insect pest mainly have loquat first mite, wood louse, aphid, yellow caterpillar, oriental fruit months, boat moth, Longicorn etc., in recent years, if the harm of loquat first mite is more universal, it has also become one of its Major Pests.If loquat first mite is mainly caused harm newly The tip and flower spike are the normal hair evil mites on loquat.It is heavy from generation to generation if loquat first mite is ominous in the etesian generation number in loquat producing region It is folded, it is larger in loquat summer shoot pumping phase generation quantity, loquat bud differentiation can be seriously affected.After perianth is done harm to, cause to wilt simultaneously Soot-like is formed, a large amount of flower spikes is caused to come off.High temperature, arid often occur heavier.
If mainly have to the control method of loquat first acarid evil at present:1. cultural control.Strengthen fertilizer and water management, never mistake Dry, overly moist enhances tree vigo(u)r, improves resistant to diseases and insects, removes fallen leaves in time, and with reference to fertilising, appropriate plowed soils reduces disease pest source Radix.It reduces to the greatest extent and makees between pear tree and loquat, mixes up or interplanting.Strengthen orchard management, overstocked branch, light leg are cut off after fruit picking Branch, prune off, it is short cut, be important to cut off sick branch, forcing down the growth of prosperous branch makes it slowly grow, and the pumping of summer shoot to be promoted to send out, is Next year high yield lays the foundation;Control nitrogenous fertilizer increases phosphorus, potash fertilizer, accomplishes Wang Shu not nitrogen fertilizer applications;Do irrigation and drainage system well, accomplish rainy season Energy draining, can pour water when non-irrigated, and treelet garden is not poured water generally in the 7-8 months, timely loose tree disk.2. biological control.When single tetranychid amount It when below 2, is prevented using Predatory Mites, while plants grass and stay grass, attract natural enemy, utilize the natural pest controlling ability of natural enemy Control the big generation of pest.3. chemical prevention.It grasps the key link period, applying pesticides are prevented, when mean univalent functions mite amount reaches 3-5 During head, if controlling 1000-1500 times of liquid etc. pair first mite using 34% 2000-2500 times of liquid of mite killing star of medicament or mite generation and preventing.
Above control method is all effective, some are even with remarkable result, such as chemical prevention.But these prevention sides Method also has the shortcomings that its is apparent, such as:Cultural control is time-consuming, and in the case where current rural area blueness strong labour power is few, problem is more prominent Go out;Biological control is also time-consuming, of high cost, and technical difficulty is big, and control effect is affected by environment larger;Chemical prevention is although effect Fruit is good, but pollutes residual greatly, does not meet organic production requirement.And if this organic loquat vegetalitas first mite insecticide overcome with These upper shortcomings, if this organic loquat vegetalitas first mite insecticide raw material is taken from free of contamination plant and crop, natural decomposition Soon, the residence time is short, easy to use, easy to operate, at low cost, and insecticidal effect is notable, without any chemical contamination, has complied fully with The requirement of machine Loquat growth.
Invention content
If the purpose of the present invention is intended to provide a kind of organic loquat vegetalitas first mite insecticide, this insecticide is easy to use, Easy to operate, at low cost, insecticidal effect is notable.If another object of the present invention is intended to provide above-mentioned organic loquat vegetalitas first mite The preferable application method of insecticide.The purpose of the present invention is what is be accomplished by the following way:
The insecticide of the present invention is by fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine is brewed forms, The middle fresh herb of tripterygium wilfordii is mainly used as auxiliary agent, plays a part of to promote its insecticidal effect;Human urine is used as brewed solvent.Inventor studies It was found that good synergistic function can be played by adding in the fresh herb of auxiliary agent tripterygium wilfordii.
If need it is emphasized that the fresh wormwood overground part of this organic loquat vegetalitas first mite insecticide, gold green pepper fresh fruit, The fresh herb of tripterygium wilfordii, human urine should all be selected from the free of contamination areas of environmental conditions such as soil, water, air, fresh wormwood overground part, Huang The mountain area of chemical fertilizer, chemical pesticide was not used in golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii.Otherwise, the quality that organic loquat will be influenced.This If organic loquat vegetalitas first mite method for producing insecticide is:Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, Human urine is in addition to above-mentioned described should all selecting comes from the free of contamination area of the environmental conditions such as soil, water, air, and soil, water body are with having Machine fertilizer etc. also wants heavy metal free to pollute.First by fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii matter by weight during preparation Amount is impregnated than being put into a certain amount of human urine.
Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine four components weight mass ratio for 1.0~ 2.0:0.5~1.5:1.5~2.5:3.0~6.0.More than the fresh wormwood overground part of four components, gold green pepper fresh fruit, tripterygium wilfordii it is fresh complete Careless, human urine most preferred weight mass ratio is 1.5:1.0:2.0:4.5.Fresh wormwood overground part, gold green pepper fresh fruit, tripterygium wilfordii are fresh Herb, human urine are calculated by fresh weight mass ratio, and human urine is calculated by the weight mass ratio of 1~30d.Fresh wormwood overground part, gold green pepper are fresh The fresh herb of fruit, tripterygium wilfordii, human urine four components quality by weight than mixing, 25~50L is big, and 20~40d is impregnated in plastic barrel capping (It not sealed during immersion), with 3~4 layers of filtered through gauze after 20~40d, if filtrate is this organic loquat vegetalitas first mite Insecticide, plastic bag sealing packaging, can preserve 2-3.If if kill first mite using this organic loquat vegetalitas first mite insecticide When, it need to ensure that all sites such as leaf, branch bar have been sprayed onto position.Desinsection dosage generally presses 1.0 with filtrate:2.0 are watered dilution spray Mist, with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position, if pair first mite can carry out Preferably kill.The production of this insecticide is upper easy to use, and easy to operate, at low cost, insecticidal effect is notable, and the shelf-life is longer, uses Plastic bag sealing is packed, and can be factory produced, and forms product.The present invention select fresh wormwood overground part, gold green pepper fresh fruit and The fresh herb of auxiliary agent tripterygium wilfordii, brewed medium human urine employ the compatibility of Suitable weight mass ratio, are configured to commercial vegetative insecticidal Agent, with significant insecticidal effect.It is the comparative test of its insecticidal effect below.
If organic loquat vegetalitas first mite insecticide of the present invention has no the report of similar product at present, the present invention is invention The result of the test that people is obtained by experimental study for many years with production practices, and be verified in the production of long period, Effect is preferable.And the formula of the present invention is also higher with technology content, dosage form is relatively stablized, and raw material sources enrich, easy to operate, such as Commodity are developed into, it is cheap to producing for organic loquat.Easy to use in production, insecticidal effect is notable, uses Cost is relatively low, is a kind of botanical pesticide of the good organic loquat of production.If organic loquat vegetalitas first mite insecticide is matched It can be factory produced, be killed if developing and using and will not only increase a kind of novel organic loquat vegetalitas first mite on the market Worm agent will also provide a new solution route to produce the problem of organic loquat deinsectization is difficult at present.
The present invention preparation method be:It first will be on fresh wormwood overground part, gold green pepper fresh fruit and the fresh herb of tripterygium wilfordii during preparation Soil, water shake off, do not need to wash, water smashs into gold green pepper fresh fruit to pieces irregular graininess or bulk after doing(Size is without tight Lattice requirement), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, fresh wormwood overground part, gold green pepper The fresh herb of fresh fruit, tripterygium wilfordii, four component of human urine weight mass ratio be 1.0~2.0:0.5~1.5:1.5~2.5:3.0~ 6.0, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrels of 25~50L 20~40d is impregnated in capping(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this is organic after 20~40d If loquat vegetalitas first mite insecticide, plastic bag sealing packaging can preserve 2-3.
The present invention application method be:If during using this organic loquat vegetalitas first mite insecticide desinsection, leaf, branch need to be ensured The all sites such as bar have been sprayed onto position.Desinsection dosage generally presses 1.0 with filtrate:2.0 are watered dilution spraying, with the leaf of worm strain, branch Bar, which is sprayed onto, to be advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position, if a pair first mite can preferably be killed.Generally Selection is before at 11 points in the fine day morning or sprays and is advisable after at 15 points in afternoon, to be ensured after spraying and interior for 24 hours not rain or do not carry out Artificial sprinkling irrigation, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is fine as possible, in plant On do not formed and be limited compared with large water drop, safe defence strictly will carry out human body defence and operation by chemical pesticide operating instruction.Spray Hayashishita cannot carry out breeding and mix battalion in 20d afterwards.If testing inspection shows using this organic loquat vegetalitas first mite insecticide, by This organic loquat of commodity produced is detected, and the main functional component of botanical pesticide does not detect.
It is that inventor achieves the object of the present invention below, constantly selects and select having for the suitable present invention by experiment If the raw material of machine loquat vegetalitas first mite insecticidal formulation and its relevant comparative test for matching done insecticidal effect.
Single group separating tests
The single effect of organic loquat first mite killing effect is tried if single group separating tests are mainly each component of qualitative test It tests.
Materials and methods
1.1.1 test material
If experiment is selected endangers more universal first mite to organic Loquat growth.Experiment is with single dose:Fresh wormwood overground part, Huang Four golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine components.Wherein human urine is brewed medium.
Test method
The preparation of test material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected From the free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii do not make The mountain area of used chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.First by fresh wormwood overground part, gold during preparation Green pepper fresh fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces after doing irregular Graininess or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.You Afterwards, fresh wormwood overground part and the weight mass ratio of human urine are 1.5:4.5, it is capped with the big plastic barrels of 25~50L of human urine of 1~30d Impregnate 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging preserves 3 Year.Gold green pepper fresh fruit and the weight mass ratio of human urine are 1.0:4.5, with the big plastic barrel capping leachings of 25~50L of human urine of 1~30d Steep 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging preserves 3 years. The fresh herb of tripterygium wilfordii and the weight mass ratio of human urine are 2.0:4.5, it is impregnated with the big plastic barrel cappings of 25~50L of human urine of 1~30d 30d(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging preserves 3 years.People Urine, with the human urine of 1~30d, the big plastic barrel capping storage 30d of 25~50L(It not sealed during storage).With 3 layers of gauze after 30d Filtering, obtains its filtrate.Plastic bag sealing packaging preserves 3 years.Desinsection dosage presses 1.0 with filtrate:2.0 are watered dilution spraying, with The leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position.Selection is at 11 points in the fine day morning In the past or at 15 points in afternoon after spray, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Comparison chemistry The spraying of 34% mite killing star of pesticide, 2000 times of liquid, observes test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which also not into Pedestrian's work is sprayed.If test effect is mainly counted and statistical analysis with the percentage of its first mite death rate.Spray date is selected It is carried out before first mite is broken out if selecting.
It tests in orchard:The control of processing 1 of experiment setting 5, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-5 and CK1.
Laboratory test:The control of processing 1 of experiment setting 5, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (If referring to net has the basin of first mite and loquat fresh-cut branches and leaves), respectively handle random alignment.Each processing number is processing 6-10 and CK2.Respectively Disposition is as follows:
Processing 1 --- fresh wormwood overground part and the weight mass ratio of human urine are 1.5:4.5, with the human urine 25 of 1~30d~ 50L is big, and 30d is impregnated in plastic barrel capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag Pack preservation 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observation experiment afterwards for 24 hours Effect.Processing 2 --- gold green pepper fresh fruit and the weight mass ratio of human urine are 1.0:4.5, with the big modelings of 25~50L of human urine of 1~30d 30d is impregnated in charging basket capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packet Dress preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Place Reason 3 --- the fresh herb of tripterygium wilfordii and the weight mass ratio of human urine are 2.0:4.5, with the big plastic barrels of 25~50L of human urine of 1~30d 30d is impregnated in capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging is protected It deposits 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 4 --- with the human urine of 1~30d, the big plastic barrel capping storage 30d of 25~50L(It not sealed during storage).With 3 layers of yarn after 30d Cloth filters, and obtains its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this urine-2Dosage 1.0:2.0 it converts Water dilution spraying.Observe test effect afterwards for 24 hours.Processing 5 --- with 34% mite killing star of chemical pesticide, 2000 times of liquid 50kg.667m-2 Dosage spray, observe test effect afterwards for 24 hours.Control(CK1)--- spray tap water 50kg.667m-2Dosage, observe afterwards for 24 hours Test effect.Processing 6 --- fresh wormwood overground part and the weight mass ratio of human urine are 1.5:4.5, with the human urine 25 of 1~30d~ 50L is big, and 30d is impregnated in plastic barrel capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag Pack preservation 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observation experiment afterwards for 24 hours Effect.Processing 7 --- gold green pepper fresh fruit and the weight mass ratio of human urine are 1.0:4.5, with the big modelings of 25~50L of human urine of 1~30d 30d is impregnated in charging basket capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packet Dress preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Place Reason 8 --- the fresh herb of tripterygium wilfordii and the weight mass ratio of human urine are 2.0:4.5, with the big plastic barrels of 25~50L of human urine of 1~30d 30d is impregnated in capping(It not sealed during immersion), with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging is protected It deposits 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 9 --- with the human urine of 1~30d, the big plastic barrel capping storage 30d of 25~50L(It not sealed during storage).With 3 layers of yarn after 30d Cloth filters, and obtains its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this urine-2Dosage 1.0:2.0 it converts Water dilution spraying.Observe test effect afterwards for 24 hours.Processing 10 --- with 34% mite killing star of chemical pesticide, 2000 times of liquid 50kg.667m-2 Dosage spray, observe test effect afterwards for 24 hours.Control(CK2)--- spray tap water 50kg.667m-2Dosage, observe afterwards for 24 hours Test effect.
Statistical analysis
SAS is used to test data(6.12)It is for statistical analysis.Result of the test shows(Table 1), the processing of orchard experiment If 1-3, the 5 first mite death rate are significantly higher than 4 and CK1 of processing, if handling the 4 first mite death rate with CK1 without significant difference, processing If the 5 first mite death rate is significantly higher than other processing.It is described above, fresh wormwood overground part, gold green pepper fresh fruit, the thunder of orchard experiment If the first mite death rate of the public fresh herb of rattan is all significantly higher than human urine and CK1, but substantially less than 2000 times of 34% mite killing star of chemical pesticide The processing 5 of liquid, if the first mite death rate of human urine processing and CK1 are without significant difference.
If 1. single group separating tests of table --- the death rate of first mite(Unit:%)
Processing The death rate Processing The death rate
Processing 1 20.40±3.41bc Processing 6 23.64±1.03b
Processing 2 19.57±0.86c Processing 7 21.80±2.16c
Processing 3 22.49±2.94b Processing 8 24.73±0.97b
Processing 4 1.23±0.83d Processing 9 2.61±0.51d
Processing 5 96.37±7.84a Processing 10 98.03±4.69a
CK1 0.92±0.39d CK2 1.97±0.40d
Note:Lowercase is horizontal for 0.05, and the different letters of same row represent significant difference, similarly hereinafter.
If the processing 6-8 of laboratory test, the 10 first mite death rate are significantly higher than 9 and CK2 of processing, if handling 9 first mite The death rate and CK2 are without significant difference, if the first mite death rate for handling 10 is significantly higher than other processing.It is described above, laboratory examination If the first mite death rate of the fresh wormwood overground part tested, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii is all significantly higher than human urine and CK2, but The substantially less than processing 10 of 34% 2000 times of liquid of mite killing star of chemical pesticide, if the first mite death rate of human urine processing and CK2 are without significance difference It is different.
Fresh wormwood overground part, gold green pepper fresh fruit, Thunder God are shown with laboratory test by the experiment of more than one pack system orchard If the first mite death rate of the fresh herb of rattan is all significantly higher than human urine processing and CK1, CK2, but substantially less than 34% mite killing star of chemical pesticide The processing of 2000 times of liquid.The death rate of CK1, the CK2 of human urine processing with spraying tap water are without significant difference.
Compatibility mechanism
Each ingredients is tested to killing the organic more universal first of loquat of harm if compatibility mechanism is mainly qualitative and quantitative The composite effect experiment of mite.
Materials and methods
2.1.1 test material
If experiment is selected endangers more universal first mite to organic Loquat growth.Experiment ingredients is fresh wormwood overground part, Huang Four golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine components.Wherein the fresh herb of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected From the free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii do not make The mountain area of used chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.First by fresh wormwood overground part, gold during preparation Green pepper fresh fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces after doing irregular Graininess or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.You Afterwards, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.0~2.0:0.5 ~1.5:1.5~2.5:3.0~6.0, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine mass ratio by weight Mixing, 25~50L is big, and 20~40d is impregnated in plastic barrel capping(It not sealed during immersion), with 3~4 layers of gauze mistake after 20~40d Filter, if filtrate is this organic loquat vegetalitas first mite insecticide, plastic bag sealing packaging is killed with the vegetalitas for preserving 3 years Worm agent is experiment insecticide stoste.Desinsection dosage presses 1.0 with filtrate:2.0 are watered dilution spraying, equal with the leaf of worm strain, branch bar Being sprayed onto is advisable, and need to ensure that all sites such as leaf, branch bar have been sprayed onto position.Selection before at 11 points in the fine day morning or at 15 points in afternoon with After spray, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Comparison 34% mite killing star 2000 of chemical pesticide The spraying of times liquid, observes test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Experiment effect If fruit is mainly counted and statistical analysis with the percentage of its first mite death rate.Advance if spraying time is broken out in first mite Row.
It tests in orchard:The control of processing 1 of experiment setting 4, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-4 and CK1.
Laboratory test:The control of processing 1 of experiment setting 4, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (If referring to net has the basin of first mite and loquat fresh-cut branches and leaves), respectively handle random alignment.Each processing number is processing 5-8 and CK2.Everywhere Reason situation is as follows:
Processing 1 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio It is 1.0:0.5:1.5:3.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 2 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight matter Amount is than being 1.5:1.0:2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying. Observe test effect afterwards for 24 hours.Processing 3 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine four component Weight mass ratio is 2.0:1.5:2.5:6.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution Spraying.Observe test effect afterwards for 24 hours.Processing 4 --- with 34% mite killing star of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage Spraying, observes test effect afterwards for 24 hours.Control(CK1)--- spray tap water 50kg.667m-2Dosage, for 24 hours afterwards observation experiment effect Fruit.Processing 5 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.0: 0.5:1.5:3.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Observation examination afterwards for 24 hours Test effect.Processing 6 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 7 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight quality Than being 2.0:1.5:2.5:6.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h After observe test effect.Processing 8 --- with 34% mite killing star of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage spray, for 24 hours After observe test effect.Control(CK2)--- spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.
Statistical analysis
SAS is used to test data(6.12)It is for statistical analysis.Result of the test shows(Table 2), orchard is tested everywhere If the first mite death rate of reason is significantly higher than CK1, if handling the 3 first mite death rate with processing 4 without significant difference, processing 2 and processing 3 Without significant difference.It is described above, the fresh wormwood overground part of orchard experiment, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine Weight mass ratio be 1.0~2.0:0.5~1.5:1.5~2.5:3.0~6.0, press 1.0 with this compatibility liquid stoste:2.0 it is watered Dilution spraying, if the killing effect of the effect pair first mite of orchard experiment after for 24 hours is notable, and with 34% mite killing star of chemical pesticide The spraying of 2000 times of liquid, for 24 hours after test effect substantially close to.Wherein, fresh wormwood overground part, gold green pepper fresh fruit, tripterygium wilfordii are fresh complete Careless, four component of human urine weight mass ratio is 1.5:1.0:2.0:4.5 orchard test effect be significantly better than fresh wormwood overground part, Gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.0:0.5:1.5:3.0 test effect.From Economic benefit combination insecticidal effect consider, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight It is 1.5 to measure mass ratio:1.0:2.0:If 4.5 compatibility is the preferable ingredients for killing loquat first mite.
If 2. compatibility mechanism of table --- the death rate of first mite(Unit:%)
Processing The death rate Processing The death rate
Processing 1 88.91±2.43c Processing 5 90.48±4.19b
Processing 2 95.37±1.82b Processing 6 97.30±0.97a
Processing 3 96.15±3.74ab Processing 7 96.17±3.62ab
Processing 4 98.02±2.19a Processing 8 98.46±2.80a
CK1 1.53±0.41d CK2 2.16±1.37c
If the first mite death rate respectively handled of laboratory test is significantly higher than CK2, if the first mite death rate of processing 6,8 is notable Higher than processing 5, the death rate of 6-7 is handled without significant difference.It is described above, the fresh wormwood overground part of laboratory test, gold green pepper The fresh herb of fresh fruit, tripterygium wilfordii, four component of human urine weight mass ratio be 1.0~2.0:0.5~1.5:1.5~2.5:3.0~ 6.0, press 1.0 with this compatibility liquid stoste:2.0 are watered dilution spraying, if the killing of the effect pair first mite of the laboratory test after for 24 hours Effect is notable, with being sprayed with 34% mite killing star of chemical pesticide, 2000 times of liquid, for 24 hours after test effect substantially close to.Wherein, fresh Chinese mugwort Meadow top, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5 reality Test room test effect be significantly better than fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight quality Than being 1.0:0.5:1.5:3.0 test effect, but with fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine four The weight mass ratio of component is 2.0:1.5:2.5:6.0 effect is without significant difference, and with 34% mite killing star of chemical pesticide The spraying of 2000 times of liquid, for 24 hours after test effect without significant difference.Consider from economic benefit combination insecticidal effect, fresh wormwood is on the ground Portion, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5 compatibility is to kill If the preferable ingredients of first of going out mite.
Above experiment is it is also shown that fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, the examination of four component compatibility of human urine If the first mite death rate tested(Table 2)It is significantly higher than fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine If the first mite death rate of single dose experiment(Table 1).It is indicated above that fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, people Four component compatibilities are urinated with preferable synergistic function.
Dosetest
Dosetest is mainly that qualitative and quantitative tests each dosage to endanger organic loquat more universal to killing If the effect test of first mite.
Test material
If experiment is selected endangers more universal first mite to organic Loquat growth.Experiment ingredients is fresh wormwood overground part, Huang Four golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine components.Wherein the fresh herb of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected From the free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii do not make The mountain area of used chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.First by fresh wormwood overground part, gold during preparation Green pepper fresh fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces after doing irregular Graininess or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.You Afterwards, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0: 4.5, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrels of 25~50L 20~40d is impregnated in capping(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this is organic after 20~40d If loquat vegetalitas first mite insecticide, plastic bag sealing packaging, to preserve the botanical pesticide of 3 years as experiment raw insecticide Liquid.Desinsection dosage presses 1.0 with filtrate:2.0 are watered dilution spraying, and with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure leaf, branch The all sites such as bar have been sprayed onto position.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, is ensured for 24 hours after spraying It does not rain inside, during which also without manually spraying.Comparison is sprayed with 34% mite killing star of chemical pesticide, 2000 times of liquid, for 24 hours observation examination afterwards Test effect, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.If test effect is mainly dead with its first mite The percentage of rate is died to count and statistical analysis.Spraying time carries out before if first mite is broken out.
It tests in orchard:The control of processing 1 of experiment setting 4, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-4 and CK1.
Laboratory test:The control of processing 1 of experiment setting 4, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (If referring to net has the basin of first mite and loquat fresh-cut branches and leaves), respectively handle random alignment.Each processing number is processing 5-8 and CK2.Everywhere Reason situation is as follows:
Processing 1 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio It is 1.5:1.0:2.0:4.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 2 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight matter Amount is than being 1.5:1.0:2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying. Observe test effect afterwards for 24 hours.Processing 3 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine four component Weight mass ratio is 1.5:1.0:2.0:4.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution Spraying.Observe test effect afterwards for 24 hours.Processing 4 --- press 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2Agent Amount spraying, observes test effect afterwards for 24 hours.Control(CK1)--- spray tap water 50kg.667m-2Dosage, for 24 hours afterwards observation experiment Effect.Processing 5 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 6 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight quality Than being 1.5:1.0:2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h After observe test effect.Processing 7 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight Mass ratio is 1.5:1.0:2.0:4.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying. Observe test effect afterwards for 24 hours.Processing 8 --- press 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2Dosage spray Mist observes test effect afterwards for 24 hours.Control(CK2)--- spray tap water 50kg.667m-2 Dosage, for 24 hours afterwards observation experiment effect Fruit.
Statistical analysis
SAS is used to test data(6.12)It is for statistical analysis.Result of the test shows(Table 3), the processing of orchard experiment If the first mite death rate of 1-4 is all remarkably higher than CK1.If the first mite death rate of processing 2-4 is significantly higher than processing 1, handle between 3-4 Without significant difference;Processing 2 is significantly higher than processing 1.It is described above, fresh wormwood overground part, gold green pepper fresh fruit, the Thunder God of orchard experiment The fresh herb of rattan, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5, press 50 kg.667m with this compatibility liquid stoste-2 With 60kg.667m-2Dosage 1.0:2.0 are watered dilution spraying, if the killing effect of the orchard test effect pair first mite after for 24 hours Significantly, without significant difference between the two.60kg.667m-2Dosage 1.0:2.0 are watered dilution spraying with using 34% mite killing of chemical pesticide 2000 times of liquid sprayings of star, the test effect observed afterwards for 24 hours is without significant difference.Consider from economic benefit combination insecticidal effect, compatibility Liquid stoste presses 50kg.667m-2If dosage be kill first mite preferable dosage.
If 3. dosetest of table --- the death rate of first mite(Unit:%)
Processing The death rate Processing The death rate
Processing 1 91.58±2.49c Processing 5 93.17±2.41b
Processing 2 95.37±1.43b Processing 6 97.30±0.84ab
Processing 3 96.04±0.82ab Processing 7 98.05±3.40a
Processing 4 98.02±2.09a Processing 8 98.46±2.19a
CK1 1.53±0.37d CK2 2.16±1.28c
If the first mite death rate of the processing 5-8 of laboratory test is all remarkably higher than CK2.If handle the first mite death rate of 7-8 It is significantly higher than processing 5, handles between 6-7 without significant difference, handles between 5-6 without significant difference.It is described above, laboratory test Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0: 4.5, press 40-60kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after laboratory test If the killing effect of effect pair first mite is notable, 50-60kg.667m-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after reality If the killing effect of room test effect pair first mite is tested with being sprayed with 34% mite killing star of chemical pesticide, 2000 times of liquid, the examination observed afterwards for 24 hours Effect is tested without significant difference.Wherein, compatibility liquid stoste presses 50kg.667m-2The laboratory test of dosage is with obvious effects to be better than compatibility Liquid stoste presses 40kg.667m-2The test effect of dosage, but press 60kg.667m with compatibility liquid stoste-2The test effect base of dosage This is identical.Consider from economic benefit combination insecticidal effect, compatibility liquid stoste presses 50kg.667m-2If dosage be kill first mite Preferable dosage.
Above experiment is it is also shown that fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, the examination of four component compatibility of human urine The death rate of the insecticidal effect tested even first mite(Table 2-3)It is fresh complete to be significantly higher than fresh wormwood overground part, gold green pepper fresh fruit, tripterygium wilfordii Grass, the death rate of four component single dose of human urine experiment(Table 1).It is indicated above that fresh wormwood overground part, gold green pepper fresh fruit, tripterygium wilfordii are fresh Herb, four component compatibility of human urine have preferable synergistic function.
Auxiliary agent is tested
If auxiliary agent experiment is mainly the synergistic function that qualitative and quantitative experiment auxiliary agent kills entire ingredients first mite.
Materials and methods
4.1.1 test material
If experiment is selected endangers more universal first mite to organic Loquat growth.Experiment ingredients is fresh wormwood overground part, Huang Four golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine components.Wherein the fresh herb of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected From the free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii do not make The mountain area of used chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.First by fresh wormwood overground part, gold during preparation Green pepper fresh fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces after doing irregular Graininess or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.You Afterwards, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0: 4.5, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrels of 25~50L 20~40d is impregnated in capping(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this is organic after 20~40d If loquat vegetalitas first mite insecticide, plastic bag sealing packaging, to preserve the botanical pesticide of 3 years as experiment raw insecticide Liquid.Desinsection dosage, with filtrate 50kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection before at 11 points in the fine day morning or under Spray after 15 points of noon, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Comparison chemical pesticide 34% kills The spraying of 2000 times of liquid of mite star, observes test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which also without artificial mark spraying It fills.If test effect is mainly counted and statistical analysis with the percentage of its first mite death rate.If spraying time is in first It is carried out before mite outburst.
It tests in orchard:The control of processing 1 of experiment setting 3, each handles 3 repetitions(Including CK), it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-3 and CK1.
Laboratory test:The control of processing 1 of experiment setting 3, each handles 3 repetitions(Including CK), it is each to repeat 1 basin (If referring to net has the basin of first mite and loquat fresh-cut branches and leaves), respectively handle random alignment.Each processing number is processing 4-6 and CK2.Everywhere Reason situation is as follows:
Processing 1 --- fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio It is 1.5:1.0:2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 2 --- fresh wormwood overground part, gold green pepper fresh fruit, three component of human urine weight mass ratio be 1.5: 1.0: 4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Observation experiment is imitated afterwards for 24 hours Fruit.Processing 3 --- press 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2Dosage spray, for 24 hours afterwards observation experiment Effect.Control(CK1)--- spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.Processing 4 --- fresh wormwood Overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5.Matched with this 5 liquid stostes press 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 5 --- it is fresh Wormwood overground part, gold green pepper fresh fruit, three component of human urine weight mass ratio be 1.5:1.0: 4.5.It is pressed with this compatibility liquid stoste 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 6 --- with chemical pesticide 34% 2000 times of liquid of mite killing star press 50kg.667m-2Dosage spray, observe test effect afterwards for 24 hours.Control(CK2)--- spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.
Statistical analysis
SAS is used to test data(6.12)It is for statistical analysis.Result of the test shows(Table 4), the processing of orchard experiment If the first mite death rate of 1-3 is significantly higher than CK1, if the first mite death rate for handling 2 is substantially less than processing 1,3, but be significantly higher than CK1.It is described above, the fresh wormwood overground part of compatibility of no auxiliary agent, gold green pepper fresh fruit, three component of human urine weight mass ratio be 1.5: 1.0:4.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after orchard experiment If the death rate of effect pair first mite be substantially less than and have the fresh wormwood overground part of compatibility, gold green pepper fresh fruit, tripterygium wilfordii of auxiliary agent fresh complete Careless, four component of human urine weight mass ratio is 1.5:1.0:2.0:4.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying, for 24 hours the death rate of rear orchard experiment;Also significantly lower than use 2000 times of 34% mite killing star of chemical pesticide Liquid 50kg.667m-2Dosage spray for 24 hours after orchard experiment the death rate.
If 4. auxiliary agent of table is tested --- the death rate of first mite(Unit:%)
Processing The death rate Processing The death rate
Processing 1 95.37±1.37b Processing 4 97.30±0.93a
Processing 2 53.19±4.09c Processing 5 59.17±3.70b
Processing 3 98.02±2.40a Processing 6 98.46±1.97a
CK1 1.53±0.41d CK2 2.16±1.17c
If the first mite death rate of the processing 4-6 of laboratory test is significantly higher than CK2, if handle 5 the first mite death rate it is notable Less than processing 4,6, but it is significantly higher than CK2.It is described above, the fresh wormwood overground part of compatibility of no auxiliary agent, gold green pepper fresh fruit, human urine three The weight mass ratio of component is 1.5:1.0:4.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 be watered it is dilute Spraying is released, if the death rate of the effect pair first mite of the laboratory test after for 24 hours is substantially less than the fresh wormwood ground of compatibility for having auxiliary agent Portion, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5, with this compatibility liquid Stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying, for 24 hours the death rate of rear laboratory test;Also significantly lower than With 34% mite killing star of chemical pesticide, 2000 times of liquid 50kg.667m-2Dosage spray for 24 hours after laboratory test the death rate.
Above experiments have shown that(Table 4), the difference on effect of adding assistant and not adding assistant is notable, and the significant effect of adding assistant is better than The not effect of adding assistant, not its significant effect of adding assistant reduction.It is notable to illustrate that the fresh herb of auxiliary agent tripterygium wilfordii has in this compatibility Collaboration and synergistic effect.
Experiment is it is also shown that the effect of ingredients(Table 3-4)It is each single dose effect(Table 1)1-4 times or so.So after compatibility Effect be not simple each single dose effect addition.Compatibility mechanism is tested to from single dose to test to auxiliary agent again(Table 1-4), as a result Show the fresh wormwood overground part of compatibility, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5: 1.0:2.0:4.5 compatibility liquid stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered the ingredients of dilution spraying, are imitated from economy It is preferably to select that benefit, which combines insecticidal effect consideration,.Experiments have shown that with fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, The compatibility agent of four component of human urine, which sprays, does not detect its main component wormwood element, capsaicine, wilfordine in the commodity loquat of rear 30d Wait ingredients.
This brewed dose is stomach poison and action of contace poison, if toxic component wormwood element, capsaicine, wilfordine etc. are with first mite If during feeding together into its upset in vivo its polypide metabolism cause be poisoned to death or directly from polypide body surface stomata invade first mite Lead to internal metabolic disorder in vivo and cause and be poisoned to death or draw from joint cell gap intrusion of the polypide without the non-keratinization of wax Joint is played to benumb internal metabolic disorder and finally result in death.
Wormwood(Artemisia argyi H. Lév. & Vaniot)Be composite family sagebruss, perennial herb or slightly into Suffruticose, plant have enhanced aroma.Stem list is raw or a small number of, high 80-150cm, and brown or grey yellowish-brown have apparent vertical rib, base Portion's slightly lignifying, top herbaceous stem, and have a small number of short branches, the long 3-5cm of branch;Stem, branch are by grey spider's thread shape pubescence.Main root is bright Aobvious, slightly thick long, diameter reaches 1.5cm, and lateral root is more;Often there are accumbency underground root-like stock and nutrition branch.Leaf ground paper matter, above by canescence Pubescence, and have white gland point and small concave point, the back side is close by the close villus of canescence spider's thread shape;Basal leaf has long handle, and the florescence withers; The lower portion of the stem leaf subcircular or width egg shape, pinniform drastic crack have 2-3 pieces of sliver, sliver ellipse or the oblong of falling ovate per side, often Sliver has 2-3 pieces small carnassial tooth, it is dry after back side master, lateral vein be mostly dark brown or rust, the long 0.5-0.8 cm of petiole;Middle leaf ovum Shape, triangular shape be avette or nearly diamond shape, long 5-8 cm, wide 4-7 cm, and one(To two)Pinniform drastic crack is returned to partly splitting, per side sliver 2-3 Piece, sliver is avette, ovum shape lanceolar or lanceolar, no longer long 2.5-5 cm, wide 1.5-2 cm, division or every side have 1-2 pieces to lack Tooth, leaf base wide wedge shape is gradually narrow into short handle, and vein is apparent, overleaf raised, rust when doing, the long 0.2-0.5 cm of petiole, base portion Usually without pseudostipule or minimum pseudostipule;Upper leaf partly splits with bract leaf pinniform, it is shallow split 3 drastic cracks or 3 it is shallow split or regardless of It splits, and is ellipse, long ellipticity lanceolar, lanceolar or wire lanceolar.Capitulum ellipse, diameter 2.5-3.0mm, Without stalk or closely without stalk, often several pieces to more than 10 pieces are lined up small-sized spike or compound spike on branch, and led on stem Normal recomposition is narrow, steeple shape panicle, and rear capitulum is spent to have a down dip;3-4 layers of phyllary, imbricate arrangement, outer layer are total Bract is small, herbaceous stem, avette or narrow avette, and the back side is close by canescence spider's thread shape wool, and edge film quality, middle level phyllary is compared with outer layer Long, long avette, the back side is by spider's thread shape wool, and internal layer phyllary matter is thin, and the back side is closely hairless;Receptacle of inflorescence is small;Female flower 6-10, corolla Narrow tubulose, eaves portion have 2 carnassial tooths, and purple, style is elongated, stretch out outside corolla and very grow, apex 2 is pitched;Hermaphrodite flower 8-12, corolla tubulose Or there are gland point in goblet shape, outside, eaves portion purple, anther is narrow linear, and apex adjunct point, Yangtze River Delta shape, base portion has unobvious Small cusp, style and corolla are near isometric or slightly longer than corolla, apex 2 are pitched, and are bent outwardly after spending, fork section shape, and ciliation. Achene long avette or oblong.The flowering fruit bearing stage 9-10 months.It is distributed in Asia and European Region.Aerial part contains a series of sequiterpene Analog derivative, such as peaceful, the blue sesame oil Azulene of wormwood element, wormwood, arborescin lactone.
Capsicum(Capsicum annuum L.)It is Solanaceae, Capsicum 1 year or limited herbaceos perennial.It is domestic peppery Green pepper principal item has gold green pepper, qi xing paprika, extra large day green pepper etc..The nearly hairless or micro- raw pubescence of caulis capsici, branch slightly zigzag warpage.Leaf Alternate, branch top section are not extended into twin or fasciation shape, and square round shape is avette, avette or ovum shape lanceolar, full edge, and top is short gradually Sharp or anxious point, the narrow wedge shape of base portion;Flower Dan Sheng, bows vertical;Calyx cup-shaped, not notable 5 tooth;Corolla white, sliver are avette;Anther ash is purple Color.Carpopodium is more sturdy, bows vertical;The long finger-like of fruit, top is tapering and normal bending, green during prematurity, it is ripe after into red, orange Or aubergine, taste are peppery.The flat kidney shape of seed, it is faint yellow.Alkaloid, pigment, grease type are mainly contained in pepper fruit, wherein, it is peppery Green pepper alkaloid is the piquancy component of capsicum, is mainly included:Capsaicine (Capsaicin), dihydrocapsaicin (Dihydrocapsaicin), nordihydrocapsaicin (Nordihydrocapsaicin), homocapsaicin (Homocapsaicin), Homodihydrocapsaicin(Homodihydrocapsaicin)And trace element, vegetable protein etc.;Contained pigment composition in capsicum Predominantly capsicum red pigment(Also referred to as capsanthin), capsorubin etc.;Contain Solanidine in pepper seed (solatubine)Deng, also some containing kryptoxanthin (cryptoxenthin), carrotene (carotene), vitamin C, lemon Acid, tartaric acid, malic acid etc..
Tripterygium wilfordii(Tripterygium wilfordii Hook. f.), Celastraceae, tripterygium plant, liana shrub, Main product is in China, Korea, Japan, and China is distributed mainly on various regions and southwest on the south the Yangtze river basin, and main product is in Fujian, Zhejiang The ground such as river, Hubei, Hunan, Anhui, Henan.It is born in in the shade how wet hillside, mountain valley, small stream side bushes.Happiness more cool place Hillside is most preferably grown with the deep sand soil of slant acidity, soil layer or yellow soil.Bitter, pungent, cool in nature, big poison.Thunder God Calamus liana Shrub, high 1~3m, sprig brownish red have 4~6 thin ribs, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, Rectangular ellipse or avette, long 4~7.5cm, wide 3~4cm, the anxious point or short tapering of apex, base portion wealthy wedge shape or circle, edge has Serration, 4~7 pairs of lateral vein, up to leaf margin after it is slightly upper curved;Petiole grows 5~8mm, close by rust hair.Circular cone cyme is narrower, Long 5~7cm, wide 3-4cm usually have 3~5 branches.The long round shape of samara, long 1~1.5cm, 1~1.2cm of diameter, seed buttress shaft Shape, up to 10mm.Tripterygium wilfordii is mainly containing wilfordine, celastrus orbiculatus benzyl amide, Triptolide etc..
Specific embodiment
Implementation below and embodiment are intended to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
The preparation of material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected and be come from The free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii were not used The mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.It is first that fresh wormwood overground part, gold green pepper is fresh during preparation Fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces irregular particle after doing Shape or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrel capping leachings of 25~50L Steep 20~40d(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this organic loquat is planted after 20~40d If physical property first mite insecticide, plastic bag sealing packaging can preserve 2~3 years.
If usage time selection sprays before organic loquat first mite is broken out, early prevention can be also carried out.In use, use filtrate 40kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0: 2.0:4.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is:If the first mite death rate 91.58%, with pressing 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2 The insecticidal effect of dosage spray only differ 6.44%, imitated substantially close to the desinsection of chemical pesticide 34% mite killing star, 2000 times of liquid Fruit.
Embodiment 2:
The preparation of material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected and be come from The free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii were not used The mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.It is first that fresh wormwood overground part, gold green pepper is fresh during preparation Fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces irregular particle after doing Shape or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrel capping leachings of 25~50L Steep 20~40d(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this organic loquat is planted after 20~40d If physical property first mite insecticide, plastic bag sealing packaging can preserve 2~3 years.
If usage time selection sprays before organic loquat first mite is broken out, early prevention can be also carried out.In use, use filtrate 50kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0: 2.0:4.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is:If the first mite death rate 95.37%, with pressing 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2 The insecticidal effect of dosage spray only differ 2.65%, imitated substantially close to the desinsection of chemical pesticide 34% mite killing star, 2000 times of liquid Fruit.
Embodiment 3:
The preparation of material:The fresh wormwood overground part of material, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine should all be selected and be come from The free of contamination area of the environmental conditions such as soil, water, air, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii were not used The mountain area of chemical fertilizer, chemical pesticide.Otherwise, the quality that organic loquat will be influenced.It is first that fresh wormwood overground part, gold green pepper is fresh during preparation Fruit and soil, water on the fresh herb of tripterygium wilfordii are shaked off, and do not need to wash, and water smashs into gold green pepper fresh fruit to pieces irregular particle after doing Shape or bulk(Size is without strict demand), fresh wormwood overground part and the fresh herb of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0:2.0:4.5, it is fresh Wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine quality by weight are than mixing, the big plastic barrel capping leachings of 25~50L Steep 20~40d(It not sealed during immersion), with 3~4 layers of filtered through gauze, filtrate it is that this organic loquat is planted after 20~40d If physical property first mite insecticide, plastic bag sealing packaging can preserve 2~3 years.
If usage time selection sprays before organic loquat first mite is broken out, early prevention can be also carried out.In use, use filtrate 60kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, four component of human urine weight mass ratio be 1.5:1.0: 2.0:4.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is:If the first mite death rate 96.04%, with pressing 50kg.667m with 34% mite killing star of chemical pesticide, 2000 times of liquid-2 The insecticidal effect of dosage spray only differ 1.98%, imitated substantially close to the desinsection of chemical pesticide 34% mite killing star, 2000 times of liquid Fruit.

Claims (3)

1. if a kind of organic loquat vegetalitas first mite insecticide, which is characterized in that if organic loquat vegetalitas first mite desinsection Agent is by fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine compatibility is brewed forms, wherein fresh wormwood overground part, Huang Golden green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine compatibility weight ratio be 1.0~2.0:0.5~1.5:1.5~2.5:3.0~ 6.0, fresh wormwood overground part, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii are counted weight by fresh material, and human urine is according to the human urine for storing 1~30d Weight calculation.
2. if a kind of organic loquat vegetalitas first mite insecticide according to claim 1, which is characterized in that fresh wormwood is on the ground Portion, gold green pepper fresh fruit, the fresh herb of tripterygium wilfordii, human urine compatibility weight ratio be 1.5:1.0:2.0:4.5.
3. if a kind of application method of organic loquat vegetalitas first mite insecticide as described in claim 1, which is characterized in that If first mite population number does not have to spray before great outburst, by 40~60kg667m-2Dosage 1.0:2.0 are watered dilution spraying, Droplet is fine as possible, is limited with not formed on plant compared with large water drop, need to ensure that leaf, branch bar portion position have been sprayed onto position, in fine day It sprays before at 11 points in the morning or after at 15 points in afternoon, to ensure interior for 24 hours not rainy after spraying or is not sprayed manually, if It is interior for 24 hours to have rained or carried out artificial sprinkling irrigation, it need to be sprayed again.
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