CN105901032B - A kind of organic loquat vegetalitas wood louse insecticide and its application method - Google Patents

A kind of organic loquat vegetalitas wood louse insecticide and its application method Download PDF

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CN105901032B
CN105901032B CN201610447445.3A CN201610447445A CN105901032B CN 105901032 B CN105901032 B CN 105901032B CN 201610447445 A CN201610447445 A CN 201610447445A CN 105901032 B CN105901032 B CN 105901032B
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fresh
leaf
wood louse
loquat
human urine
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CN105901032A (en
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郭兆武
邵亚楠
杨洁
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Changsha University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
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  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract

The invention discloses a kind of organic loquat vegetalitas wood louse insecticide and its application methods, the botanical pesticide is by masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf compatibility human urine of tripterygium wilfordii is brewed forms, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 2.5~3.5:1.0~2.0:3.5~4.5:7.0~10.0, it is preferably in a proportion of 3.0:1.5:4.0:8.5, four components mass ratio by weight calculates, and wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, has synergistic function between ingredients.This brewed dose notable to the killing effect of organic loquat wood louse.40~60kg.667m is pressed in this agent‑2Dosage 1.0:2.0 are watered dilution spraying.This brewed dose of stable dosage forms, raw material sources enrich, easy to use in production, easy to operate, at low cost, without any chemical contamination, comply fully with the requirement of organic Loquat growth.

Description

A kind of organic loquat vegetalitas wood louse insecticide and its application method
Technical field
The invention belongs to agriculture practical new technology research and development and applications, and the present invention relates to a kind of organic loquat vegetalitas wood louses to kill The research and development of worm agent and production application method.
Background technology
LoquatEriobotrya japonica(Thunb.) Lindl ］ it is the rose family, Eriobotrya, originate in China The southeast is gained the name due to leaf-shaped is like Chinese lute musical instrument, and flower can be used as medicine.Loquat is evergreen dungarunga, sets high 3-5m, and highest can Up to 10m or so；Blade is big and grows, thick and have fine hair, and in oblong, shape such as Chinese lute, keratin, lanceolar falls lanceolar, the ovum that falls Shape or oval oblong, long 12-30cm, wide 3-9cm, the anxious point or tapering of apex, base portion is wedge-shaped or gradually narrow into petiole, top edge Have a thin sawtooth, base portion full edge, above it is bright, it is wrinkle more, below dense taupe brown villus, 11-21 pairs of lateral vein；Petiole it is short or there are few Handle, long 6-10mm have taupe brown villus；Stipule bores shape, long 1-1.5cm, the anxious point of apex, hairiness.Sprig is sturdy, yellowish-brown, close Get rusty color or taupe brown villus.Panicle basidixed, long 10-19cm, tool are spent more；Total bennet and the dense rust villus of bennet, flower Obstruct long 2-8mm；Bract bores shape, long 2-5mm, dense rust villus；Flower diameter 12-20mm；Calyx tube tazza shape, long 4-5mm, sepal There are rust villus in triangular egg, long 2-3mm, the anxious point of apex, calyx tube and sepal outside；Petal white, oblong or avette, long 5- 9mm, wide 4-6mm, base portion tool pawl, there is rust villus；Stamen 20, considerably shorter than petal, the extension of filigree base portion；Style 5, from life, column Head's shape, hairless, there is rust pubescence on ovary top, and there are 2 ovules in Room 5 per room.Fruit is spherical or oblong, diameter 2-5cm are yellow Color or orange colour have rust pubescence, come off soon outside；Seed 1-5, spherical or oblate spheroid, diameter 1-1.5cm, brown is bright, Kind mulberry paper matter.The month at florescence 10-12, the fruiting period 5-6 months.Also bunchy is hung on the tree ripe loquat fruit, each long 3-5cm of fruit, Into round, oval or long shape " Chinese lute shape ".Loquat surface is by villous, and dark green when underdone, stronger, aromatic odor is denseer. Crust is generally faint yellow after maturation, also there is that color is deeper, close to orange-red.Pulp is soft and succulence, can be divided mainly into white And orange two kinds.Different from most of fruit tree, loquat is in autumn or early winter blooms, and fruit is ripe in spring to early summer, than other Fruit is early, therefore is " solely standby seasonal pathogens,exopathogens person in fruit tree " by title.Fruit soft and succulency, sweet and sour palatability is full of nutrition, leaf, flower, Fruit, benevolence can control cough.The tough and tensile exquisiteness of timber is engraving, makes the high-quality timber of top-grade furniture.Loquat is mainly distributed on sweet Respectful, Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei, Hunan, Sichuan, Yunnan, Guizhou, Guangxi, Guangdong, Fujian, Taiwan Etc. ground.The wide row cultivation in various regions, there are wild species in Sichuan, Hubei.Also there are cultivation in Japan, India, Vietnam, Burma, Thailand, Indonesia Training.
Loquat is a kind of raw economic plants of drought of high-yield and high-efficiency, and there are extensive cultivation in various regions, with the planting site industrial structure Adjustment, cultivated area have the gesture of continuous expansion.In recent years, loquat pest and disease damage was on the rise, it has also become development loquat One big obstacle of fruit industry production.If loquat insect pest mainly have loquat wood louse, first mite, aphid, yellow caterpillar, oriental fruit months, boat moth, Longicorn etc., in recent years, the harm of loquat wood louse are more universal, it has also become one of its Major Pests.
Loquat wood louse belongs to Homoptera Psyllidae, and concentrating on young sprout, petiole, blade with adult and nymph sucks juice.Quilt The distortion of evil blade vein, generates withered spot, and gradual blackening at blade face shrinkage, induces sooty mould and fallen leaves.Wood louse is in recent years in loquat It is upper to occur generally, mostly generation occurs within 1 year, occur in arid season heavier.Its position of causing harm is concentrated mainly on petiole base after fruit picking Crack in, secreting mucus, anti-rain drop erosion power is strong.Worm sources largely accumulate, the warm winter, high temperature and drought, are conducive to the generation of wood louse, Particularly there is pear tree to mix up or the garden of interplanting, occur it is even more serious, aggrieved serious orchard have worm newly slightly rate up to 80% with On.
Mainly have to the control method of loquat wood louse insect pest at present：1. cultural control.Strengthen fertilizer and water management, never overdrying, Overly moist enhances tree vigo(u)r, improves resistant to diseases and insects, removes fallen leaves in time, and with reference to fertilising, appropriate plowed soils reduces disease pest source base Number.It reduces to the greatest extent and makees between pear tree and loquat, mixes up or interplanting.Strengthen orchard management, overstocked branch, light leg are cut off after fruit picking Branch, prune off, it is short cut, be important to cut off sick branch, forcing down the growth of prosperous branch makes it slowly grow, and the pumping of summer shoot to be promoted to send out, is Next year high yield lays the foundation；Control nitrogenous fertilizer increases phosphorus, potash fertilizer, accomplishes Wang Shu not nitrogen fertilizer applications；Do irrigation and drainage system well, accomplish rainy season Energy draining, can pour water when non-irrigated, and treelet garden is not poured water generally in the 7-8 months, timely loose tree disk.2nd, physical control.It is installed in field Trapping lamp using adult pests such as light trap wood louse, yellow caterpillars, utilizes yellow card's trap aphid.3. biological control.Kind grass stays Grass attracts natural enemy, utilizes the big generation of the natural pest controlling capability control pest of natural enemy.4. chemical prevention.Taking out the periods such as tip phase is The key of prevention, grasps the key link period, and applying pesticides are prevented, and in the medication of low instar larvae phase, 20% wood louse can be selected in medicament The safe 1500 times of liquid of 8000 times of liquid or 10% An Lvbao of 800-1200 times of liquid of emulsion for cleaning, 25% Acker are prevented.
Above control method is all effective, some are even with remarkable result, such as chemical prevention.But these prevention sides Method also has the shortcomings that its is apparent, such as：Cultural control is time-consuming, and in the case where current rural area blueness strong labour power is few, problem is more prominent Go out；Physical control can only control population quantity to a certain extent, environmental condition suitable for when its insect pest be difficult to avoid completely；Biology Cost accounting is high, and technical difficulty is big, and control effect is affected by environment larger；Chemical prevention is although work well, pollution residual Greatly, organic production requirement is not met.And this organic loquat vegetalitas wood louse insecticide overcomes the above shortcoming, this organic Pi Rake vegetalitas wood louse insecticide raw material is taken from free of contamination plant and crop, and natural decomposition is fast, and the residence time is short, user Just, easy to operate, at low cost, insecticidal effect is notable, without any chemical contamination, complies fully with the requirement of organic Loquat growth.
Invention content
The purpose of the present invention is intended to provide a kind of organic loquat vegetalitas wood louse insecticide, this insecticide is easy to use, behaviour Make simply, at low cost, insecticidal effect is notable.Another object of the present invention is intended to provide above-mentioned organic loquat vegetalitas wood louse desinsection The preferable application method of agent.The purpose of the present invention is what is be accomplished by the following way：
The insecticide of the present invention is by masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine is brewed forms, wherein The fresh cauline leaf of tripterygium wilfordii is mainly used as auxiliary agent, plays a part of to promote its insecticidal effect；Human urine is used as brewed solvent.Inventor studies hair Existing, good synergistic function can be played by adding in the fresh cauline leaf of auxiliary agent tripterygium wilfordii.It needs it is emphasized that this organic loquat plant Property wood louse insecticide masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine should all select from soil, water, air etc. Environmental condition is pollution-free, and the area of chemical fertilizer, chemical pesticide was not used in growth environment, and industrial pollution and heavy metal are waited without opening a mine Pollution.Otherwise, the quality that organic loquat will be influenced.Originally organic loquat vegetalitas wood louse method for producing insecticide is：During preparation First masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii mass ratio by weight are put into a certain amount of human urine and impregnated. Masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine four components weight mass ratio be 2.5~3.5:1.0~ 2.0:3.5~4.5:7.0~10.0.More than four components most preferred weight mass ratio be 3.0:1.5:4.0:8.5.Masson pine Fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine are calculated by fresh weight mass ratio, and human urine is based on the weight mass ratio of 1~30d It calculates.Four components quality by weight is than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20 With 3~4 layers of filtered through gauze after~40d, filtrate is this organic loquat vegetalitas wood louse insecticide, plastic bag sealing packaging, 2-3 can be preserved.When killing wood louse using this organic loquat vegetalitas wood louse insecticide, it need to ensure that leaf, branch bar portion position have sprayed In place.Desinsection dosage generally presses 1.0 with filtrate:2.0 are watered dilution spraying, and with the leaf of worm strain, branch bar is sprayed onto is advisable, and needs to protect Barrier leaf, branch bar portion position have been sprayed onto position, and wood louse can preferably be killed.The production of this insecticide is upper easy to use, operation letter Single, at low cost, insecticidal effect is notable, and the shelf-life is longer, is packed with plastic bag sealing, can be factory produced, and forms product.
The present invention selects masson pine fresh leaf, qi xing paprika fresh fruit and the fresh cauline leaf of auxiliary agent tripterygium wilfordii, brewed medium human urine to employ The compatibility of Suitable weight mass ratio is configured to commercial botanical pesticide, with significant insecticidal effect.It is its desinsection below The comparative test of effect.
Organic loquat vegetalitas wood louse insecticide of the present invention has no the report of similar product at present, and the present invention is inventor It by the result of the test that experimental study for many years and production practices are obtained, and has been verified, has imitated in the production of long period Fruit is preferable.And the formula of the present invention is also higher with technology content, dosage form is relatively stablized, and raw material sources enrich, easy to operate, such as open Commodity are sent out into, it is cheap to producing for organic loquat.Easy to use in production, insecticidal effect is notable, using into This is relatively low, is a kind of botanical pesticide of the good organic loquat of production.Organic loquat vegetalitas wood louse insecticidal formulation can It being factory produced, utilization will not only increase a kind of novel organic loquat vegetalitas wood louse insecticide on the market, A new solution route will also be provided to produce the problem of organic loquat deinsectization is difficult at present.
The present invention preparation method be：It first will be on masson pine fresh leaf, qi xing paprika fresh fruit and the fresh cauline leaf of tripterygium wilfordii during preparation Soil, water and other foreign matters etc. are shaked off, and do not need to wash, if band dew etc. during harvesting, to carry out the system of material again after water is done It is standby, smash into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, masson pine fresh leaf and tripterygium wilfordii Fresh cauline leaf is cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine four component Weight mass ratio is 2.5~3.5:1.0~2.0:3.5~4.5:7.0~10.0, masson pine fresh leaf, qi xing paprika fresh fruit, tripterygium wilfordii Fresh cauline leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, With 3~4 layers of filtered through gauze after 20~40d, filtrate is this organic loquat vegetalitas wood louse insecticide, plastic bag sealing packet Dress, can preserve 2-3.
The present invention application method be：When being prevented using this organic loquat vegetalitas wood louse insecticide, need to ensure leaf, Branch bar portion position has been sprayed onto position.Desinsection dosage generally presses 1.0 with filtrate:2.0 are watered dilution spraying, equal with the leaf of worm strain, branch bar Being sprayed onto is advisable, and need to ensure that leaf, branch bar portion position have been sprayed onto position, preferably killing effect can be carried out to wood louse.It is typically chosen in fine Spraying before at 11 points in its morning or after at 15 points in afternoon is advisable, and to ensure interior for 24 hours not rainy after spraying or not sprayed manually, If interior for 24 hours rained or carried out artificial sprinkling irrigation, need to be sprayed again.Droplet is fine as possible, not formed on plant It is limited compared with large water drop, safe defence strictly will carry out human body defence and operation by chemical pesticide operating instruction.Woods in 20d after spray Under cannot carry out breeding and mix battalion.Testing inspection shows, using this organic loquat vegetalitas wood louse insecticide, thus to produce The organic loquat of commodity is detected it, and the main functional component of botanical pesticide does not detect.It is inventor below It achieves the object of the present invention, constantly selects and select the organic loquat vegetalitas wood louse insecticide for being suitble to the present invention by experiment The raw material of formula and its relevant comparative test for matching done insecticidal effect.
Single group separating tests
Single group separating tests are mainly that qualitative and quantitative tests single effect of each component to organic loquat wood louse killing effect Fruit is tested.
Materials and methods
1.1.1 test material
Experiment, which is selected, endangers organic Loquat growth more universal wood louse.Experiment is with single dose：Masson pine fresh leaf, qi xing paprika Four the fresh cauline leaf of fresh fruit, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine, which should all be selected, to be come Pollution-free from environmental conditions such as soil, water, air, growth environment was not used the area of chemical fertilizer, chemical pesticide, without the works such as open a mine Industry pollutes and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit during preparation It is shaked off with soil, water and other foreign matters on the fresh cauline leaf of tripterygium wilfordii etc., does not need to wash, if band dew etc. during harvesting, will treat water The preparation of material is carried out after dry again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, Masson pine fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, the weight mass ratio of masson pine fresh leaf and human urine is 3.0:8.5, impregnate 30d with the big plastic barrel cappings of 25~50L of human urine of 1~30d（It not sealed during immersion）, with 3 layers after 30d Filtered through gauze obtains its filtrate.Plastic bag sealing packaging preserves 3 years.Qi xing paprika fresh fruit and the weight mass ratio of human urine are 1.5: 8.5, impregnate 30d with the big plastic barrel cappings of 25~50L of human urine of 1~30d（It not sealed during immersion）, with 3 layers of gauze after 30d Filtering, obtains its filtrate.Plastic bag sealing packaging preserves 3 years.The fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 4.0: 8.5, impregnate 30d with the big plastic barrel cappings of 25~50L of human urine of 1~30d（It not sealed during immersion）, with 3 layers of gauze after 30d Filtering, obtains its filtrate.Plastic bag sealing packaging preserves 3 years.Human urine, with the human urine of 1~30d, the big plastic barrel cappings of 25~50L Store 30d（It not sealed during storage）.With 3 layers of filtered through gauze after 30d, its filtrate is obtained.Plastic bag sealing packaging preserves 3 Year.Desinsection dosage presses 1.0 with filtrate:2.0 are watered dilution spraying, and with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure leaf, branch Bar portion position has been sprayed onto position.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, ensured after spraying it is interior for 24 hours not under Rain, during which also without manually spraying.Comparison 20% wood louse emulsion for cleaning of chemical pesticide（Its commodity missible oil contains Amitraz, pungent Sulphur phosphorus and lambda-cyhalothrin）The spraying of 1200 times of liquid, observes test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which Without manually spraying.Test effect is mainly counted and statistical analysis with the percentage of its wood louse death rate.Spray date Selection carries out before wood louse is broken out.
It tests in orchard：The control of processing 1 of experiment setting 5, each handles 3 repetitions（Including CK）, it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-5 and CK1.
Laboratory test：The control of processing 1 of experiment setting 5, each handles 3 repetitions（Including CK）, it is each to repeat 1 basin （Referring to net has the basin of wood louse and loquat fresh-cut branches and leaves）, respectively handle random alignment.Each processing number is processing 6-10 and CK2.
Each disposition is as follows：
Processing 1 --- the weight mass ratio of masson pine fresh leaf and human urine is 3.0:8.5, with 25~50L of human urine of 1~30d 30d is impregnated in big plastic barrel capping（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag is close Package dress preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observation experiment is imitated afterwards for 24 hours Fruit.Processing 2 --- qi xing paprika fresh fruit and the weight mass ratio of human urine are 1.5:8.5, with the big plastics of 25~50L of human urine of 1~30d 30d is impregnated in bucket capping（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing is packed It preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 3 --- the fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 4.0:8.5, added with the big plastic barrels of 25~50L of human urine of 1~30d Lid impregnates 30d（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging preserves 3 Year.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 4 --- With the human urine of 1~30d, the big plastic barrel capping storage 30d of 25~50L（It not sealed during storage）.With 3 layers of gauze mistake after 30d Filter, obtains its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this urine-2Dosage 1.0:2.0 be watered it is dilute Release spraying.Observe test effect afterwards for 24 hours.Processing 5 --- with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid 50kg.667m-2 Dosage spray, observe test effect afterwards for 24 hours.Control（CK1）--- spray tap water 50kg.667m-2Dosage, observe afterwards for 24 hours Test effect.Processing 6 --- the weight mass ratio of masson pine fresh leaf and human urine is 3.0:8.5, with 25~50L of human urine of 1~30d 30d is impregnated in big plastic barrel capping（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Polybag is close Package dress preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observation experiment is imitated afterwards for 24 hours Fruit.Processing 7 --- qi xing paprika fresh fruit and the weight mass ratio of human urine are 1.5:8.5, with the big plastics of 25~50L of human urine of 1~30d 30d is impregnated in bucket capping（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing is packed It preserves 3 years.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 8 --- the fresh cauline leaf of tripterygium wilfordii and the weight mass ratio of human urine are 4.0:8.5, added with the big plastic barrels of 25~50L of human urine of 1~30d Lid impregnates 30d（It not sealed during immersion）, with 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing packaging preserves 3 Year.50kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 9 --- With the human urine of 1~30d, the big plastic barrel capping storage 30d of 25~50L（It not sealed during storage）.With 3 layers of gauze mistake after 30d Filter, obtains its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this urine-2Dosage 1.0:2.0 be watered it is dilute Release spraying.Observe test effect afterwards for 24 hours.Processing 10 --- with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid 50kg.667m-2 Dosage spray, observe test effect afterwards for 24 hours.Control（CK2）--- spray tap water 50kg.667m-2Dosage, observe afterwards for 24 hours Test effect.
Statistical analysis
SAS is used to test data（6.12）It is for statistical analysis.Result of the test shows（Table 1）, the processing of orchard experiment 1-3, the 5 wood louse death rate are significantly higher than 4 and CK1 of processing, handle the 4 wood louse death rate with CK1 without significant difference, handle 5 The wood louse death rate is significantly higher than other processing.Described above, the masson pine fresh leaf of orchard experiment, qi xing paprika fresh fruit, tripterygium wilfordii are fresh The wood louse death rate of cauline leaf is all significantly higher than human urine and CK1, but substantially less than 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid Processing 5, human urine processing is with the wood louse death rate of CK1 without significant difference.
1. single group separating tests of table --- the death rate of wood louse（Unit：%）
Processing The death rate Processing The death rate
Processing 1 16.97±3.69b Processing 6 21.57±0.81c
Processing 2 15.27±2.28bc Processing 7 23.91±1.69bc
Processing 3 13.91±4.09c Processing 8 2530±2.17b
Processing 4 0.81±0.26d Processing 9 3.42±1.19d
Processing 5 96.04±6.83a Processing 10 98.27±3.28a
CK1 1.24±0.41d CK2 2.64±0.81d
Note：Lowercase is horizontal for 0.05, and the different letters of same row represent significant difference, similarly hereinafter.
The processing 6-8 of laboratory test, the 10 wood louse death rate are significantly higher than 9 and CK2 of processing, handle 9 wood louse death For rate with CK2 without significant difference, the wood louse death rate for handling 10 is significantly higher than other processing.It is described above, the horse of laboratory test Tail pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii the wood louse death rate be all significantly higher than human urine and CK2, but be substantially less than and change It learns to farm the processing 10 of 20% 1200 times of liquid of wood louse emulsion for cleaning of medicine, the wood louse death rate of human urine processing and CK2 are without significant difference.
Masson pine fresh leaf, qi xing paprika fresh fruit, tripterygium wilfordii are shown with laboratory test by the experiment of more than one pack system orchard The wood louse death rate of fresh cauline leaf is all significantly higher than human urine processing and CK1, CK2, but substantially less than 20% wood louse of chemical pesticide is only newborn The processing of oily 1200 times of liquid.The death rate of CK1, the CK2 of human urine processing with spraying tap water are without significant difference.
Compatibility mechanism
Compatibility mechanism is mainly that qualitative and quantitative tests each ingredients wood louse more universal to killing the organic loquat of harm Composite effect experiment.
Materials and methods
2.1.1 test material
Experiment, which is selected, endangers organic Loquat growth more universal wood louse.Experiment is masson pine fresh leaf, qi xing paprika with ingredients Four the fresh cauline leaf of fresh fruit, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine, which should all be selected, to be come Pollution-free from environmental conditions such as soil, water, air, growth environment was not used the area of chemical fertilizer, chemical pesticide, without the works such as open a mine Industry pollutes and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit during preparation It is shaked off with soil, water and other foreign matters on the fresh cauline leaf of tripterygium wilfordii etc., does not need to wash, if band dew etc. during harvesting, will treat water The preparation of material is carried out after dry again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, Masson pine fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, tripterygium wilfordii are fresh Cauline leaf, four component of human urine weight mass ratio be 2.5~3.5:1.0~2.0:3.5~4.5:7.0~10.0, masson pine fresh leaf, The fresh cauline leaf of qi xing paprika fresh fruit, tripterygium wilfordii, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping （It not sealed during immersion）, with 3~4 layers of filtered through gauze, filtrate it is that this organic loquat vegetalitas wood louse kills after 20~40d Worm agent, plastic bag sealing packaging, to preserve the botanical pesticide of 3 years as experiment insecticide stoste.Desinsection dosage, uses filtrate By 1.0:2.0 are watered dilution spraying, and with the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure that leaf, branch bar portion position have been sprayed onto position. Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, ensured after spraying it is interior for 24 hours do not rain, during which also without people Work is sprayed.Comparison 20% wood louse emulsion for cleaning of chemical pesticide（Its commodity missible oil contains Amitraz, phoxim and lambda-cyhalothrin） The spraying of 1200 times of liquid, observes test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Examination Effect is tested mainly to be counted and statistical analysis with the percentage of its wood louse death rate.Spraying time is broken out in wood louse to advance Row.
It tests in orchard：The control of processing 1 of experiment setting 4, each handles 3 repetitions（Including CK）, it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-4 and CK1.
Laboratory test：The control of processing 1 of experiment setting 4, each handles 3 repetitions（Including CK）, it is each to repeat 1 basin （Referring to net has the basin of wood louse and loquat fresh-cut branches and leaves）, respectively handle random alignment.Each processing number is processing 5-8 and CK2.
Each disposition is as follows：
Processing 1 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 2.5:1.0:3.5:7.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 2 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio It is 3.0:1.5:4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 3 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight quality Than being 3.5:2.0:4.5:10.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h After observe test effect.Processing 4 --- with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid 50kg.667m-2Dosage spray Mist observes test effect afterwards for 24 hours.Control（CK1）--- spray tap water 50kg.667m-2Dosage, for 24 hours afterwards observation experiment effect Fruit.Processing 5 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 2.5: 1.0:3.5:7.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Observation examination afterwards for 24 hours Test effect.Processing 6 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 it is watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 7 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio It is 3.5:2.0:4.5:10.0.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 8 --- with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid 50kg.667m-2Dosage spray, Observe test effect afterwards for 24 hours.Control（CK2）--- spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.
Statistical analysis
SAS is used to test data（6.12）It is for statistical analysis.Result of the test shows（Table 2）, orchard is tested everywhere The wood louse death rate of reason is significantly higher than CK1, handles the 3 wood louse death rate with processing 4 without significant difference, processing 2 is with processing 3 without aobvious Write difference, processing 2 and 1 significant difference of processing.Described above, the masson pine fresh leaf of orchard experiment, qi xing paprika fresh fruit, tripterygium wilfordii are fresh Cauline leaf, four component of human urine weight mass ratio be 2.5~3.5:1.0~2.0:3.5~4.5:7.0~10.0, with this compatibility liquid Stoste presses 1.0:2.0 are watered dilution spraying, for 24 hours after orchard experiment effect it is notable to the killing effect of wood louse, and with chemistry The spraying of 20% wood louse emulsion for cleaning of pesticide, 1200 times of liquid, for 24 hours after test effect substantially close to.Wherein, masson pine fresh leaf, qi xing paprika The fresh cauline leaf of fresh fruit, tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5 orchard test effect is significantly excellent In masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 2.5:1.0:3.5:7.0 Test effect.Consider from economic benefit combination insecticidal effect, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine The weight mass ratio of four components is 3.0:1.5:4.0:8.5 compatibility is the preferable ingredients for killing loquat wood louse.
2. compatibility mechanism of table --- the death rate of wood louse（Unit：%）
Processing The death rate Processing The death rate
Processing 1 85.27±4.16c Processing 5 88.04±2.76c
Processing 2 94.86±2.70b Processing 6 96.17±1.34b
Processing 3 95.27±1.81ab Processing 7 97.53±4.05ab
Processing 4 97.16±2.07a Processing 8 99.51±2.42a
CK1 0.72±0.31d CK2 3.49±0.93d
The wood louse death rate respectively handled of laboratory test is significantly higher than CK2, and the wood louse death rate for handling 6-8 is significantly higher than Processing 5 handles the death rate between 7-8 without significant difference, handles the death rate between 6-7 without significant difference.It is described above, laboratory The masson pine fresh leaf of experiment, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 2.5~3.5:1.0 ~2.0:3.5~4.5:7.0~10.0, press 1.0 with this compatibility liquid stoste:2.0 be watered dilution spraying, for 24 hours after laboratory examination The effect tested is notable to the killing effect of wood louse, with being sprayed with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid, for 24 hours after examination Test effect substantially close to.Wherein, the weight mass ratio of masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine It is 3.0:1.5:4.0:8.5 laboratory test significant effect better than masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, The weight mass ratio of four component of human urine is 2.5:1.0:3.5:7.0 test effect, but with masson pine fresh leaf, qi xing paprika fresh fruit, The fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.5:2.0:4.5:10.0 effect without significant difference, this The weight mass ratio of four components is 3.5:2.0:4.5:10.0 effect again with 20% wood louse emulsion for cleaning of chemical pesticide The spraying of 1200 times of liquid, for 24 hours after test effect without significant difference.Consider from economic benefit combination insecticidal effect, masson pine fresh leaf, The fresh cauline leaf of qi xing paprika fresh fruit, tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5 compatibility is to kill wood The preferable ingredients of lice.
Above experiment is it is also shown that masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, the experiment of four component compatibility of human urine The wood louse death rate（Table 2）It is significantly higher than masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, the examination of four component single dose of human urine The wood louse death rate tested（Table 1）.It is indicated above that four groups of masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine distribution 5 have preferable synergistic function.
Dosetest
Dosetest is mainly that qualitative and quantitative tests each dosage to endanger organic loquat more universal to killing The effect test of wood louse.
Test material
Experiment, which is selected, endangers organic Loquat growth more universal wood louse.Experiment is masson pine fresh leaf, qi xing paprika with ingredients Four the fresh cauline leaf of fresh fruit, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine, which should all be selected, to be come Pollution-free from environmental conditions such as soil, water, air, growth environment was not used the area of chemical fertilizer, chemical pesticide, without the works such as open a mine Industry pollutes and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit during preparation It is shaked off with soil, water and other foreign matters on the fresh cauline leaf of tripterygium wilfordii etc., does not need to wash, if band dew etc. during harvesting, will treat water The preparation of material is carried out after dry again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, Masson pine fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, tripterygium wilfordii are fresh Cauline leaf, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20~ With 3~4 layers of filtered through gauze after 40d, filtrate is this organic loquat vegetalitas wood louse insecticide, plastic bag sealing packaging, with The botanical pesticide for preserving 3 years is experiment insecticide stoste.Desinsection dosage presses 1.0 with filtrate:2.0 are watered dilution spraying, With the leaf of worm strain, branch bar is sprayed onto is advisable, and need to ensure that leaf, branch bar portion position have been sprayed onto position.Selection is before at 11 points in the fine day morning Or at 15 points in afternoon after spray, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Comparison chemical pesticide 20% 1200 times of wood louse emulsion for cleaning liquid spray, observe test effect afterwards for 24 hours, ensured after spraying it is interior for 24 hours do not rain, during which also not into Pedestrian's work is sprayed.Test effect is mainly counted and statistical analysis with the percentage of its wood louse death rate.Spraying time It is carried out before wood louse is broken out.
It tests in orchard：The control of processing 1 of experiment setting 4, each handles 3 repetitions（Including CK）, it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-4 and CK1.
Laboratory test：The control of processing 1 of experiment setting 4, each handles 3 repetitions（Including CK）, it is each to repeat 1 basin （Referring to net has the basin of wood louse and loquat fresh-cut branches and leaves）, respectively handle random alignment.Each processing number is processing 5-8 and CK2.
Each disposition is as follows：
Processing 1 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 2 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio It is 3.0:1.5:4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 3 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight quality Than being 3.0:1.5:4.0:8.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.24h After observe test effect.Processing 4 --- press 50kg.667m with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid-2Dosage spray Mist observes test effect afterwards for 24 hours.Control（CK1）--- spray tap water 50kg.667m-2Dosage, for 24 hours afterwards observation experiment effect Fruit.Processing 5 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0: 1.5:4.0:8.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Observation examination afterwards for 24 hours Test effect.Processing 6 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 7 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio It is 3.0:1.5:4.0:8.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.After for 24 hours Observe test effect.Processing 8 --- press 50kg.667m with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid-2Dosage spray Mist observes test effect afterwards for 24 hours.Control（CK2）--- spray tap water 50kg.667m-2 Dosage, for 24 hours afterwards observation experiment effect Fruit.
Statistical analysis
SAS is used to test data（6.12）It is for statistical analysis.Result of the test shows（Table 3）, the processing of orchard experiment The wood louse death rate of 1-4 is all remarkably higher than CK1.The wood louse death rate of processing 2-4 is significantly higher than processing 1, and nothing is aobvious between handling 3-4 Write difference, handle 2-3 between without significant difference；Processing 2 is significantly higher than processing 1.Described above, the masson pine of orchard experiment is fresh Leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, with this compatibility liquid Stoste presses 50kg.667m-2With 60kg.667m-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after orchard test effect pair The killing effect of wood louse is notable, without significant difference between the two.60kg.667m-2Dosage 1.0:2.0 are watered dilution spraying and use 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid sprayings, the test effect observed afterwards for 24 hours is without significant difference.From economic benefit knot It closes insecticidal effect to consider, compatibility liquid stoste presses 50kg.667m-2Dosage be kill wood louse preferable dosage.
3. dosetest of table --- the death rate of wood louse（Unit：%）
Processing The death rate Processing The death rate
Processing 1 89.17±3.471c Processing 5 94.61±2.80b
Processing 2 94.86±2.87b Processing 6 96.17±1.69b
Processing 3 95.82±0.83ab Processing 7 97.31±4.17ab
Processing 4 97.16±2.49a Processing 8 99.51±1.94a
CK1 0.72±0.27d CK2 3.49±1.10c
The wood louse death rate of the processing 5-8 of laboratory test is all remarkably higher than CK2.The wood louse death rate for handling 7-8 is notable It higher than processing 5, handles between 7-8 without significant difference, handles between 5-7 without significant difference.It is described above, the horse of laboratory test Tail pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, use this Compatibility liquid stoste presses 40-60kg.667m-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after laboratory test effect to wood The killing effect of lice is notable, 60kg.667m-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after laboratory test effect pair The killing effect of wood louse with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid with being sprayed, and the test effect observed afterwards for 24 hours is without aobvious Write difference.Wherein, compatibility liquid stoste presses 50kg.667m-2The laboratory test of dosage is with obvious effects to press better than compatibility liquid stoste 40kg.667m-2The test effect of dosage, but press 60kg.667m with compatibility liquid stoste-2The test effect of dosage is without significant difference. Consider from economic benefit combination insecticidal effect, compatibility liquid stoste presses 50kg.667m-2Dosage be kill wood louse preferable dosage.
Above experiment is it is also shown that masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, the experiment of four component compatibility of human urine Insecticidal effect, that is, wood louse the death rate（Table 2-3）It is significantly higher than masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, people Urinate the death rate of four component single doses experiment（Table 1）.It is indicated above that masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, people Four component compatibilities are urinated with preferable synergistic function.
Auxiliary agent is tested
Auxiliary agent experiment is mainly the synergistic function that qualitative and quantitative experiment auxiliary agent kills entire ingredients wood louse.
Materials and methods
4.1.1 test material
Experiment, which is selected, endangers organic Loquat growth more universal wood louse.Experiment is masson pine fresh leaf, qi xing paprika with ingredients Four the fresh cauline leaf of fresh fruit, tripterygium wilfordii, human urine components.Wherein the fresh cauline leaf of tripterygium wilfordii is auxiliary agent, and human urine is brewed medium.
Test method
The preparation of test material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine, which should all be selected, to be come Pollution-free from environmental conditions such as soil, water, air, growth environment was not used the area of chemical fertilizer, chemical pesticide, without the works such as open a mine Industry pollutes and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit during preparation It is shaked off with soil, water and other foreign matters on the fresh cauline leaf of tripterygium wilfordii etc., does not need to wash, if band dew etc. during harvesting, will treat water The preparation of material is carried out after dry again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, Masson pine fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, tripterygium wilfordii are fresh Cauline leaf, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20~ With 3~4 layers of filtered through gauze after 40d, filtrate is this organic loquat vegetalitas wood louse insecticide, plastic bag sealing packaging, with The botanical pesticide for preserving 3 years is experiment insecticide stoste.Desinsection dosage, with filtrate 50kg.667m-2By 1.0:2.0 it converts Water dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, ensured after spraying it is interior for 24 hours do not rain, the phase Between also without manually spraying.Comparison is sprayed with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid, and effect is tested in observation afterwards for 24 hours Fruit, ensured after spraying it is interior for 24 hours do not rain, during which also without manually spraying.Test effect is mainly with its wood louse death rate Percentage counts and statistical analysis.Spraying time carries out before wood louse is broken out.
It tests in orchard：The control of processing 1 of experiment setting 3, each handles 3 repetitions（Including CK）, it is each to repeat 1 plant, Each processing random distributed points.Each processing number is processing 1-3 and CK1.
Laboratory test：The control of processing 1 of experiment setting 3, each handles 3 repetitions（Including CK）, it is each to repeat 1 basin （Referring to net has the basin of wood louse and loquat fresh-cut branches and leaves）, respectively handle random alignment.Each processing number is processing 4-6 and CK2.
Each disposition is as follows：
Processing 1 --- masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.It sees afterwards for 24 hours Examine test effect.Processing 2 --- masson pine fresh leaf, qi xing paprika fresh fruit, three component of human urine weight mass ratio be 3.0:1.5: 8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Place Reason 3 --- press 50kg.667m with 20% wood louse emulsion for cleaning of chemical pesticide, 1200 times of liquid-2Dosage spray, for 24 hours afterwards observation experiment Effect.Control（CK1）--- spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.Processing 4 --- masson pine Fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5.With this compatibility Liquid stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 5 --- horse hair Loose fresh leaf, qi xing paprika fresh fruit, three component of human urine weight mass ratio be 3.0:1.5: 8.5.It is pressed with this compatibility liquid stoste 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying.Observe test effect afterwards for 24 hours.Processing 6 --- use chemical pesticide 20% 1200 times of wood louse emulsion for cleaning liquid presses 50kg.667m-2Dosage spray, observe test effect afterwards for 24 hours.Control（CK2）—— Spray tap water 50kg.667m-2Dosage, observe test effect afterwards for 24 hours.
Statistical analysis
SAS is used to test data（6.12）It is for statistical analysis.Result of the test shows（Table 4）, the processing of orchard experiment The wood louse death rate of 1-3 is significantly higher than CK1, and the wood louse death rate for handling 2 is substantially less than processing 1,3, but be significantly higher than CK1.With Upper explanation, the compatibility masson pine fresh leaf of no auxiliary agent, qi xing paprika fresh fruit, three component of human urine weight mass ratio be 3.0:1.5: 8.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 be watered dilution spraying, for 24 hours after orchard experiment effect Fruit is substantially less than the compatibility masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, the human urine four that have auxiliary agent to the death rate of wood louse The weight mass ratio of component is 3.0:1.5:4.0:8.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 it converts The death rate of water dilution spraying, for 24 hours rear orchard experiment；Also significantly lower than use chemical pesticide 20% wood louse emulsion for cleaning, 1200 times of liquid 50kg.667m-2Dosage spray for 24 hours after orchard experiment the death rate.
4. auxiliary agent of table is tested --- the death rate of wood louse（Unit：%）
Processing The death rate Processing The death rate
Processing 1 94.86±2.37b Processing 4 96.17±2.04b
Processing 2 47.39±6.43c Processing 5 55.82±1.90c
Processing 3 97.16±2.18a Processing 6 99.51±2.13a
CK1 0.72±0.21d CK2 3.49±1.29d
The wood louse death rate of the processing 4-6 of laboratory test is significantly higher than CK2, handles the 5 wood louse death rate and is substantially less than Processing 4,6, but it is significantly higher than CK2.It is described above, the compatibility masson pine fresh leaf of no auxiliary agent, qi xing paprika fresh fruit, three component of human urine Weight mass ratio is 3.0:1.5:8.5, press 50kg.667m with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying, The effect of laboratory test after for 24 hours, which is substantially less than the death rate of wood louse, has the compatibility masson pine fresh leaf of auxiliary agent, qi xing paprika fresh The fresh cauline leaf of fruit, tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, it is pressed with this compatibility liquid stoste 50kg.667m-2Dosage 1.0:2.0 are watered dilution spraying, for 24 hours the death rate of rear laboratory test；Also significantly lower than with chemistry 20% wood louse emulsion for cleaning of pesticide, 1200 times of liquid 50kg.667m-2Dosage spray for 24 hours after laboratory test the death rate.
Above experiments have shown that（Table 4）, the difference on effect of adding assistant and not adding assistant is notable, and the significant effect of adding assistant is better than The not effect of adding assistant, not its significant effect of adding assistant reduction.It is notable to illustrate that the fresh cauline leaf of auxiliary agent tripterygium wilfordii has in this compatibility Collaboration and synergistic effect.
Experiment is it is also shown that the effect of ingredients（Table 3-4）It is each single dose effect（Table 1）1-4 times or so.So after compatibility Effect be not simple each single dose effect addition.Compatibility mechanism is tested to from single dose to test to auxiliary agent again（Table 1-4）, as a result Show compatibility masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5: 4.0:8.5 compatibility liquid stoste presses 50kg.667m-2Dosage 1.0:2.0 are watered the ingredients of dilution spraying, from economic benefit knot It is preferably to select to close insecticidal effect consideration.Experiments have shown that with masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine four The compatibility agent of component, which sprays, does not detect its main component larch turpentine, cyanidenon, capsaicine, thunder in the commodity loquat of rear 30d The ingredients such as public rattan alkali.
This brewed dose be stomach poison and action of contace poison, toxic component larch turpentine, cyanidenon, capsaicine, tripterygium wilfordii Alkali upsets the metabolism of its polypide into it together when with wood louse feeding and causes and be poisoned to death or directly from polypide body surface stomata in vivo Lead to internal metabolic disorder in intrusion wood louse body and cause and be poisoned to death or between joint cell of the polypide without the non-keratinization of wax Gap intrusion causes joint to benumb internal metabolic disorder and finally result in death.
Masson pine（Pinus massoniana Lamb.）It is Pinaceae pine genus plant, arbor is high up to 45m, diameter of a cross-section of a tree trunk 1.3 meters above the ground 1.5m； Bark bronzing, lower part taupe are cleaved into irregular squamous block piece；Branch is open and flat or oblique exhibition, the wide turriform of tree crown or umbrella shape, branch Annual growth one is taken turns, but then usually grow two-wheeled in South Guangdong, khaki, no white powder, and rare white powder is hairless；Hibernaculum ovum Shape is cylindrical or cylindrical, brown, and top point, perula edge is Filamentous, apex point or into tapering long tip, micro- contrary flexure.Needle 2 Needle is a branch of, and dilute 3 needle is a branch of, and long 12-20cm is fine and soft, and there is spiracle line on micro- distortion, two sides, and there is serration at edge；Cross section hypodermic layer Cell monotype, first layer continuous arrangement, the second layer are formed by respective cells intermittent arrangement, resin canal about 4-8, overleaf side The raw or outside of belly also has 2 side lifes；It is brown at the beginning of leaf sheath, after crossfade into grey black, harbor.Male cone reddish tan, it is cylindrical, Curved to hang down, long 1-1.5cm, consor is in shoot lower part bud armpit, spike, long 6-15cm；The life of female cone list or 2-4 consor are near in shoot Top, lilac red, annual conelet spheroidal or oval, diameter about 2cm, brown or puce, the squama navel of top cone-scale Tool upright short thorn upwards, the squama navel of lower part cone-scale are put down blunt stingless.Cone oval or coniform oval, long 4-7 cm, diameter 2.5-4 cm have short stalk, sagging, and green before ripe, Chestnut when ripe comes off successively；The nearly square round shape obovate of middle part fruit scale or Nearly rectangle is about 3 cm；Scutum diamond shape, micro- protuberance or flat, transverse ridge is slightly bright aobvious, and squama navel nick is stingless, the person that is born in dry environment Often with extremely short thorn；The long oval of seed, long 4-6mm, the long 2-2.7 cm of fructus forsythiae；5-8 pieces of cotyledon；Long 1.2-2.4 cm；It is nascent Leaf bar shaped, long 2.5-3.6 cm, leaf margin tool sparsely grow setiform sawtooth.The month at florescence 4-5, the cone second year 10-12 months are ripe.Horse hair Loose distributed pole is wide, and north is from Henan and Southern Shandong Province, south to Guangdong and Guangxi Provinces, Hunan, Taiwan, and east is from coastal, west to central Sichuan and Guizhou, It is dispersed throughout Central China south China various regions.Generally in 600~700m of THE LOWER YANGTZE VALLEY height above sea level hereinafter, middle reaches about more than 1200m, upstream is about Below 1500m is distributed, and is the main timber-used seeds of SOUTHERN CHINA, and economic value is high.Pinus massoniana master is containing larch turpentine, sweet-scented osmanthus The secondary metabolites such as careless element, Quercetin.
Capsicum（Capsicum annuum L.）It is Solanaceae, Capsicum 1 year or limited herbaceos perennial.It is domestic peppery Green pepper principal item has qi xing paprika, gold green pepper, extra large day green pepper etc..The nearly hairless or micro- raw pubescence of caulis capsici, branch slightly zigzag warpage.Leaf Alternate, branch top section are not extended into twin or fasciation shape, and square round shape is avette, avette or ovum shape lanceolar, full edge, and top is short gradually Sharp or anxious point, the narrow wedge shape of base portion；Flower Dan Sheng, bows vertical；Calyx cup-shaped, not notable 5 tooth；Corolla white, sliver are avette；Anther ash is purple Color.Carpopodium is more sturdy, bows vertical；The long finger-like of fruit, top is tapering and normal bending, green during prematurity, it is ripe after into red, orange Or aubergine, taste are peppery.The flat kidney shape of seed, it is faint yellow.Alkaloid, pigment, grease type are mainly contained in pepper fruit, wherein, it is peppery Green pepper alkaloid is the piquancy component of capsicum, is mainly included：Capsaicine (Capsaicin), dihydrocapsaicin (Dihydrocapsaicin), nordihydrocapsaicin (Nordihydrocapsaicin), homocapsaicin (Homocapsaicin), Homodihydrocapsaicin（Homodihydrocapsaicin）And trace element, vegetable protein etc.；Contained pigment composition in capsicum Predominantly capsicum red pigment（Also referred to as capsanthin）, capsorubin etc.；Contain Solanidine in pepper seed （solatubine）Deng, also some containing kryptoxanthin (cryptoxenthin), carrotene (carotene), vitamin C, lemon Acid, tartaric acid, malic acid etc..
Tripterygium wilfordii（Tripterygium wilfordii Hook. f.）, Celastraceae, tripterygium plant, liana shrub, Main product is in China, Korea, Japan, and China is distributed mainly on various regions and southwest on the south the Yangtze river basin, and main product is in Fujian, Zhejiang The ground such as river, Hubei, Hunan, Anhui, Henan.It is born in in the shade how wet hillside, mountain valley, small stream side bushes.Happiness more cool place Hillside is most preferably grown with the deep sand soil of slant acidity, soil layer or yellow soil.Bitter, pungent, cool in nature, big poison.Thunder God Calamus liana Shrub, high 1~3m, sprig brownish red have 4~6 thin ribs, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, Rectangular ellipse or avette, long 4~7.5cm, wide 3~4cm, the anxious point or short tapering of apex, base portion wealthy wedge shape or circle, edge has Serration, 4~7 pairs of lateral vein, up to leaf margin after it is slightly upper curved；Petiole grows 5~8mm, close by rust hair.Circular cone cyme is narrower, Long 5~7cm, wide 3-4cm usually have 3~5 branches.The long round shape of samara, long 1~1.5cm, 1~1.2cm of diameter, seed buttress shaft Shape, up to 10mm.Tripterygium wilfordii is mainly containing wilfordine, celastrus orbiculatus benzyl amide, Triptolide etc..
Specific embodiment
Implementation below and embodiment are intended to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1：
The preparation of material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine should all select from soil, The environmental conditions such as water, air are pollution-free, and growth environment was not used the area of chemical fertilizer, chemical pesticide, and industry dirt is waited without opening a mine Dye and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit and thunder during preparation Soil, water and other foreign matters on the public fresh cauline leaf of rattan etc. are shaked off, and do not need to wash, be after water is done if band dew etc. during harvesting The preparation of material is carried out again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, horse hair Loose fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20~ With 3~4 layers of filtered through gauze after 40d, filtrate is this organic loquat vegetalitas wood louse insecticide, and plastic bag sealing packaging can It preserves 2~3 years.
Usage time selection sprays before organic loquat wood louse is broken out, and can also carry out early prevention.In use, use filtrate 40kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5: 4.0:8.5.40kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is：The wood louse death rate 89.17%, with being pressed with chemical pesticide 20% wood louse emulsion for cleaning, 1200 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 7.99%, substantially close to 20% wood louse emulsion for cleaning of chemical pesticide The insecticidal effect of 1200 times of liquid.
Embodiment 2：
The preparation of material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine should all select from soil, The environmental conditions such as water, air are pollution-free, and growth environment was not used the area of chemical fertilizer, chemical pesticide, and industry dirt is waited without opening a mine Dye and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit and thunder during preparation Soil, water and other foreign matters on the public fresh cauline leaf of rattan etc. are shaked off, and do not need to wash, be after water is done if band dew etc. during harvesting The preparation of material is carried out again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, horse hair Loose fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20~ With 3~4 layers of filtered through gauze after 40d, filtrate is this organic loquat vegetalitas wood louse insecticide, and plastic bag sealing packaging can It preserves 2~3 years.
Usage time selection sprays before organic loquat wood louse is broken out, and can also carry out early prevention.In use, use filtrate 50kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5: 4.0:8.5.50kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is：The wood louse death rate 94.86%, with being pressed with chemical pesticide 20% wood louse emulsion for cleaning, 1200 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 2.30%, substantially close to 20% wood louse emulsion for cleaning of chemical pesticide The insecticidal effect of 1200 times of liquid.
Embodiment 3：
The preparation of material：Material masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine should all select from soil, The environmental conditions such as water, air are pollution-free, and growth environment was not used the area of chemical fertilizer, chemical pesticide, and industry dirt is waited without opening a mine Dye and heavy metal pollution.Otherwise, the quality that organic loquat will be influenced.First by masson pine fresh leaf, qi xing paprika fresh fruit and thunder during preparation Soil, water and other foreign matters on the public fresh cauline leaf of rattan etc. are shaked off, and do not need to wash, be after water is done if band dew etc. during harvesting The preparation of material is carried out again, smashs into qi xing paprika fresh fruit to pieces irregular graininess or bulk（Size is without strict demand）, horse hair Loose fresh leaf and the fresh cauline leaf of tripterygium wilfordii are cut into the segment of 3-5cm long.Thereafter, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, four component of human urine weight mass ratio be 3.0:1.5:4.0:8.5, masson pine fresh leaf, qi xing paprika fresh fruit, the fresh stem of tripterygium wilfordii Leaf, human urine quality by weight are than mixing, and 25~50L is big, and 20~40d is impregnated in plastic barrel capping（It not sealed during immersion）, 20~ With 3~4 layers of filtered through gauze after 40d, filtrate is this organic loquat vegetalitas wood louse insecticide, and plastic bag sealing packaging can It preserves 2~3 years.
Usage time selection sprays before organic loquat wood louse is broken out, and can also carry out early prevention.In use, use filtrate 60kg.667m-2By 1.0:2.0 are watered dilution spraying.Selection sprays before at 11 points in the fine day morning or after at 15 points in afternoon, sprays After ensure it is interior for 24 hours do not rain, during which also without manually spraying, if interior for 24 hours rained or carried out artificial sprinkling irrigation, need to carry out Again it sprays.Droplet is fine as possible, and do not formed on plant compared with large water drop as degree, safe defence will be grasped strictly by chemical pesticide Make regulation and carry out human body defence and operation.Hayashishita cannot carry out breeding and mix battalion in 20d after spray.
Masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, four component of human urine weight mass ratio be 3.0:1.5: 4.0:8.5.60kg.667m is pressed with this compatibility liquid stoste-2Dosage 1.0:2.0 are watered dilution spraying.Organic Loquat-Shaped Cake after for 24 hours The insecticidal effect in garden is：The wood louse death rate 95.82%, with being pressed with chemical pesticide 20% wood louse emulsion for cleaning, 1200 times of liquid 50kg.667m-2The insecticidal effect of dosage spray only differ 1.34%, substantially close to 20% wood louse emulsion for cleaning of chemical pesticide The insecticidal effect of 1200 times of liquid.

Claims (3)

1. a kind of organic loquat vegetalitas wood louse insecticide, which is characterized in that organic loquat vegetalitas wood louse insecticide by Masson pine fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii, human urine compatibility is brewed forms, and wherein masson pine fresh leaf, qi xing paprika are fresh The fresh cauline leaf of fruit, tripterygium wilfordii, human urine compatibility mass ratio be 2.5~3.5:1.0~2.0:3.5~4.5:7.0~10.0, horse hair Loose fresh leaf, qi xing paprika fresh fruit, the fresh cauline leaf of tripterygium wilfordii press the weight Mass Calculation of fresh material, and human urine is based on the weight quality of 1~30d It calculates.
2. a kind of organic loquat vegetalitas wood louse insecticide according to claim 1, which is characterized in that masson pine fresh leaf, The fresh cauline leaf of qi xing paprika fresh fruit, tripterygium wilfordii, human urine compatibility mass ratio be 3.0:1.5:4.0:8.5.
3. a kind of application method of organic loquat vegetalitas wood louse insecticide as described in claim 1, which is characterized in that in Pi Rake wood louse population number does not have to spray before great outburst, by 40~60kg 667m-2Dosage 1.0:2.0 are watered dilution spraying, Droplet is fine as possible, is limited with not formed on plant compared with large water drop, need to ensure that leaf, branch bar portion position have been sprayed onto position, in fine day It sprays before at 11 points in the morning or after at 15 points in afternoon, to ensure interior for 24 hours not rainy after spraying or is not sprayed manually, if It is interior for 24 hours to have rained or carried out artificial sprinkling irrigation, it need to be sprayed again.
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