CN105660718A

CN105660718A - Botanical pesticide for organic lotus roots and application method of botanical pesticide

Info

Publication number: CN105660718A
Application number: CN201610033081.4A
Authority: CN
Inventors: 郭兆武; 邵亚楠; 杨洁
Original assignee: Changsha University of Science and Technology
Current assignee: Changsha University of Science and Technology
Priority date: 2016-01-19
Filing date: 2016-01-19
Publication date: 2016-06-15
Anticipated expiration: 2036-01-19
Also published as: CN105660718B

Abstract

The invention discloses a botanical pesticide for organic lotus roots and an application method of the botanical pesticide.The botanical pesticide prepared by fresh coriaria sinica maxim root, fresh tripterygium glycosides root core, fresh buddleja lindleyana root and human urine, wherein the weight ratio of the fresh coriaria sinica maxim root to the fresh tripterygium glycosides root core to the fresh buddleja lindleyana root to the human urine is 2.5-3.5:1.0-2.0:1.5-2.5:3.5-4.5, the weight ratio is preferably 3.0:1.5:2.0:4.0, the fresh buddleja lindleyana root is auxiliary, and the fresh coriaria sinica maxim root, fresh tripterygium glycosides root core, fresh buddleja lindleyana root and human urine are combined to achieve synergic effects.The botanical pesticide has the advantages that the botanical pesticide is evident in killing effect on three main pests of the organic lotus roots; 25-35kg of the botanical pesticide is used for every 667m<2> of water field through diluted spraying according to the ratio of 1.0:2.0; the botanical pesticide is stable in dosage form, rich in raw material source, convenient to use during production, simple to operate, low in cost, free of chemical pollution, and capable of completely conforming to the production requirements of the organic lotus roots.

Description

A kind of organic lotus rhizome vegetable insecticide and using method thereof

Technical field

The invention belongs to the practical new technology research and development of agricultural and application; The present invention relates to the research and development of a kind of organic lotus rhizome vegetable insecticide and production application method.

Background technology

Lotus (NelumbonuciferaG.) belonging to Dicotyledoneae, the perennial aquatic herbaceous plant of Nymphaeceae, also known as lotus, lotus property happiness warm and moist weather, originating in south, Asia, at existing more than the 3000 year cultivation history of China, all there is plantation on the ground such as Jiangsu, Zhejiang, Hubei, Hunan, Shandong, Henan, Hebei, Guangdong. Lotus is divided into lotus root lotus, seed lotus and Hua Lian. Lotus rhizome is carefully thin at first to be called close as referred to, has joint on close, and joint regeneration is close, and joint is raw fibrous root downwards, and subterraneous stem respectively saves and adventive root all occurs, and absorbs nutrition from the soil layer of table soil 20-30cm; Upwards leaf development and bennet, Xia Qiu grows latter stage, lotus whip tip is several sections of bury after enlarge into lotus root, the lotus rhizome of meaning, new strain can be germinated for the next spring. Summer-flowering, is referred to as lotus, and pattern is light red or white, large-scale, single-lobe or polyphyll. Spending rear holder to expand, form the seedpod of the lotus, interior raw most nut, is referred to as lotus seeds. The leaf of lotus rhizome is called lotus leaf, from the primary leaf that kind of lotus root is extracted out, floats on the water surface, is referred to as floating leaf. Leaf below stretches out the water surface, is called vertical leaf, and lotus whip, with starting to enlarge into leaf on the joint of lotus root, is referred to as a rear leaf. Riding over forward the less softness of new lotus root a slice and the less leaf of seta again, be called termination leaf, this is last leaf of lotus rhizome, and form is unusual. Having radial vein 19-23 bar on blade face, there is a grey community at leaf center, is called leaf navel or lotus nose, excretes for ventilation and moisture in body, and leaf epidermal cell has fine protrusions, and moisture can be stoped to stop at leaf surface. Lotus leaf is the organ manufacturing nutrition, plays a part again exchanging gas, therefore that growing of lotus root is extremely important; protect lotus leaf, it is the key capturing lotus rhizome high yield, as in peak of growing season; lotus leaf meets serious disease and pest or wind is too much bad greatly, will cause a large amount of underproduction of lotus root.

The subterraneous stem of lotus is lotus root, and can eat food also can be medicinal, often does aquatic class vegetables, and shape hypertrophy has joint, inside has tube orifice, is divided into safflower lotus root, spends lotus root in vain, fried dough twist lotus root. Safflower lotus root is tall and thin, and crust is isabelline, coarse, and moisture is few, not tender and crisp; Spending lotus root hypertrophy in vain, appearance delicacy is smooth, in silvery white, and the tender and crisp succulence of meat, sweet taste is strong; Fried dough twist lotus root starch is red, and appearance is coarse, many containing starch. Lotus root is micro-sweet and crisp, can eat raw and also can cook, and is one of conventional meal dish. Lotus root is also the plant with pharmaceutical use, and its root leaf, stamen or pistil fruit all can nourish and be used as medicine. Making powder with lotus root, can help digestion antidiarrheal, appetizing heat-clearing, and nourishing is nourished one's nature, prevention internal hemorrhage, is women and children virgin old woman, the good fluid food of valetudinarian and nourishes good treasure. Lotus root is containing abundant Vc and mineral substance, and its drug effect is of value to heart, can enhance metabolism, prevent pachylosis.Lotus is the important rural economy crop of growing area, and lotus rhizome, lotus seed are its important agricultural-food. Lotus is the aquatic cash crop of a kind of high-yield and high-efficiency, and various places have cultivates widely, and along with planting site industry restructuring, its cultivated area has the gesture of continuous expansion. In recent years, lotus rhizome disease and pest is on the rise, and has become the big obstacle that development lotus rhizome is produced.

The primary pest of lotus has aphid, big psychid, thorn moth, food root leaf beetle etc., main harm floats leaf, tender leaf, vertical leaf, leaf stalk, petal, bud etc., cause yellow leaf, poor growth, vertical leaf can not be unfolded, affecting normal plants, bud can not normally open or normal Pollination Fertilization, seriously affects lotus root lotus, seed lotus and the yield and quality spending lotus.

At present the prevention and controls of lotus rhizome insect pest is mainly contained: 1. cultural control, strictly according to the facts road wheel do, soil disinfection, select precocity, high yield, disease-resistant kind, apply fertilizers scientifically, rational close planting etc., or extract and have worm to float leaf, concentrated burn or buried etc. 2. biological control mainly utilizes the natural enemies such as ladybug, aphid parasite, wasp fly to prevent and treat. 3. physical control mainly contains lamp light trapping adult etc. 4. chemical prevention mainly uses chemical pesticide, as sprayed Provado, Decamethrin, Kui sulphur phosphorus, Aphox, Rogor, Trichlorphon, Rogor etc.

Above prevention and controls is all effective, and some even has unusual effect, such as chemical prevention. But these prevention and controls also have its obvious shortcoming, as: cultural control is time-consuming takes a lot of work, and when the blue or green strong labour power in current rural area is few, problem is more outstanding; Physical control is not only time-consuming takes a lot of work, and can only be a kind of assisting in preventing and treating measure; The single-minded selectivity of biological control is strong, can not prevent and treat comprehensively, as utilized the natural enemies such as ladybug, aphid parasite, wasp fly to prevent and treat, only that lotus rhizome aphid is effective; Although chemical prevention effect is good, but it is big to pollute residual, does not meet organic production requirement. And this organic lotus rhizome vegetable insecticide overcomes these shortcomings above, this organic lotus rhizome vegetable insecticide raw material is taken from the plant of free of contamination self-sow, its natural decomposition block, residence time is short, it may also be useful to convenient, simple to operate, cost is low, insecticidal effect is remarkable, without any chemical pollution, meets the requirement producing organic lotus rhizome completely.

Summary of the invention

The object of the present invention aims to provide a kind of organic lotus rhizome vegetable insecticide, and this sterilant is easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable. Another object of the present invention aims to provide the preferably using method of above-mentioned organic lotus rhizome vegetable insecticide. It is an object of the invention to realize in the following manner:

The vegetable insecticide of the present invention be fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate brewed become, wherein fresh of building plum grass is mainly used as auxiliary agent, plays its insecticidal effect of promotion; People's urine is used as brewed solvent. Contriver studies discovery, adds fresh of auxiliary agent building plum grass and can play good synergistic function.

Need it is emphasized that, fresh of the Coriaria sinica of this organic lotus rhizome vegetable insecticide, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine (xylem of root), building plum grass fresh root system self-sow and become, do not use the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact.

The preparation method of this organic lotus rhizome vegetable insecticide is: fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine said all should be selected from, except the free of contamination areas of envrionment conditions such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute except above-mentioned.During preparation first by fresh for Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh by weight mass ratio put into a certain amount of people urine soak.

The weight mass ratio of the Four composition of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5. The weight mass ratio most preferably of fresh of above Four composition Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine is 3.0:1.5:2.0:4.0. Fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine calculate by fresh heavy mass ratio, and people's urine calculates by the weight mass ratio of 1～30d. Fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, the Four composition of people's urine by weight mass ratio mix even, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, plastic bag sealing, can preserve 2-3. When using this organic lotus rhizome vegetable insecticide to kill lotus rhizome insect, the thin water layer that field need to stay water 2～5cm dark. Sterilant amount, generally with filtrate 25～35kg.667m^-2By 1.0:2.0 dilution spraying, lotus rhizome insect can be killed preferably. This sterilant is produced upper easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable, and the quality guaranteed period is longer, packs with plastic bag sealing, can carry out factorial praluction, forms product.

The present invention selects fresh of Coriaria sinica, the fresh radical center of trypterygine and fresh of auxiliary agent building plum grass, and brewed medium people urinates, and have employed the compatibility of suitable quality, is mixed with commercial vegetable insecticide, and it has significant insecticidal effect. This organic lotus rhizome vegetable insecticide is easy to use, simple to operate, and cost is low, and insecticidal effect is remarkable, and this is that other physical measure institutes cannot be than. The following is the comparison test of its insecticidal effect.

Organic lotus rhizome vegetable insecticide of the present invention has no the report of like product at present, and the present invention is the test-results that contriver draws through experimental study for many years and production practice, and is verified in the production of long period, and effect is better. And the formula of the present invention also to have technology content higher, formulation is more stable, abundant raw material source, easy and simple to handle, and as being developed to commodity, concerning producing organic lotus rhizome, price is cheap. In production easy to use, insecticidal effect is remarkable, it may also be useful to cost is low, be a kind of well produce organic lotus rhizome vegetable insecticide. Organic lotus rhizome plant insecticide agent prescription can carry out factorial praluction, and it develops not only will commercially increase a kind of novel organic lotus rhizome vegetable insecticide, also provide a new solution route by for producing the problem of organic lotus rhizome deinsectization poor effect at present.

The compound method of the present invention is: during preparation first by fresh for Coriaria sinica, the fresh radical center of trypterygine and Lou Mei grass fresh on earth, water shake off, do not need washing. Thereafter, the mass weight ratio that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine press mass weight than mixed even, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, and plastic bag sealing, can preserve 2-3.

The using method of the present invention is: when using this organic lotus rhizome vegetable insecticide to kill worm, the thin water layer that field need to stay water 2～5cm dark.Sterilant amount, generally with filtrate 25～35kg.667m^-2By 1.0:2.0 dilution spraying, lotus rhizome insect can be killed preferably. Before being typically chosen at 10 in fine day morning or sprayed at dusk, to be ensured after spraying and not rain in 15～20h or manually spray, if having rained in 15～20h or having carried out artificial sprinkling irrigation, need to again spray. The larva before 3 ages or nymph is selected to carry out spray medicine, the big psychid of lotus rhizome to be sprayed medicine when egg hatching, and droplet is as far as possible fine, not repeat spraying, being limited not form the globule on plant, safe defence strictly to be carried out human body defence and field operation by chemical pesticide working specification. After spray medicine, in 30d, field can not carry out the mixed battalion of breeding. Testing inspection shows, it may also be useful to this organic lotus rhizome vegetable insecticide, the organic lotus rhizome of the commodity thus produced detects, all non-detection of the main functional component of its vegetable insecticide.

The following is the object that invention artificially realizes the present invention, the comparison test of the insecticidal effect that the raw material constantly selecting and selecting organic lotus rhizome plant insecticide agent prescription of applicable the present invention by testing and relevant proportioning thereof are done.

Single component is tested

Single component tests each component of mainly qualitative test to the single effect test killing 3 kinds of organic lotus rhizome insects.

Materials and methods

1.1.1 test materials

Test is selected and organic lotus rhizome is produced serious 3 kind primary pest of causing harm: lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome thorn moth. Test single dose is: fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate four components.

Test method

The preparation of test materials: fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh root system self-sow and become, do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact. During preparation first by fresh for Coriaria sinica, building plum grass fresh and fresh of Coriaria sinica on earth, water shake off, do not need washing. Thereafter, mass weight that fresh of Coriaria sinica and people urinate than being 3.0::4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. Mass weight that the fresh radical center of trypterygine and people urinate than being 1.5:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. The mass weight of fresh of building plum grass and people's urine than being 2.0:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. People urinate, urinate with the people of 1～30d, the big plastic tank of 25～50L add lid deposit do not need between 30d(staging life seal). By 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. During field test, the thin water layer that field stays water 2～5cm dark, each community insect protected net is separated. Sterilant amount, with filtrate 25～35kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning, ensure in 15h after spraying and do not rain, also manually do not spray. Contrast chemical pesticide Rogor, by the 2000 times of liquid sprayings of 40% Rogor, every 667m²The lotus rhizome field dosage of diluent 70kg.Viewing test effect after 24h, ensures in 15h after spraying and does not rain, also manually do not spray. Test effect is mainly with its 3 kinds of primary pests: the per-cent of lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome thorn moth mortality ratio counts and statistical study. Carry out when the larva of spraying time before 3 ages or nymph.

Field test: test arranges 5 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 10m²Field area, respectively processes random alignment. Each process is numbered process 1-5 and CK1.

Pot experiment: test arranges 5 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 1 basin, respectively processes random alignment. Each process is numbered process 6-10 and CK2.

Each disposition is as follows:

Process mass weight that fresh of 1 Coriaria sinica and people urinate than being 3.0::4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process mass weight that the 2 fresh radical centers of trypterygine and people urinate than being 1.5:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. The mass weight processing fresh of 3rd floors plums grass and people's urine than being 2.0:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process 4 is urinated with the people of 1～30d, the big plastic tank of 25～50L add lid deposit do not need between 30d(staging life to seal). By 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed by this urine^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 5, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK1) water spray, viewing test effect after 24h. Process mass weight that fresh of 6 Coriaria sinicas and people urinate than being 3.0::4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process mass weight that the 7 fresh radical centers of trypterygine and people urinate than being 1.5:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. The mass weight processing fresh of 8th floors plums grass and people's urine than being 2.0:4.0, with the people of 1～30d urinate the big plastic tank of 25～50L add lid soak do not need between 30d(soak period to seal), by 3 layers of filtered through gauze after 30d, obtain its filtrate.Plastic bag sealing preserves 3 years. 25kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process 9 is urinated with the people of 1～30d, the big plastic tank of 25～50L add lid deposit do not need between 30d(staging life to seal). By 3 layers of filtered through gauze after 30d, obtain its filtrate. Plastic bag sealing preserves 3 years. 25kg.667m is pressed by this urine^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 10, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK2) water spray, viewing test effect after 24h.

Statistical study

Testing data is adopted SAS(6.12) carry out statistical study. Test-results shows (table 1), process 1-3,5,6-8,10 lotus rhizome aphid mortality ratio be significantly higher than other process with CK1, CK2, process 5, process 10 aphid mortality ratio be significantly higher than other process with CK1, CK2. More than illustrating, the aphid mortality ratio of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass is all significantly higher than people's urine and CK1, CK2, but significantly lower than process 5, the process 10 of chemical pesticide Rogor; People's urine process of field and pot experiment and the aphid mortality ratio of CK are without significant difference.

Table 1. single component is testedThe mortality ratio (unit: %) of three kinds of main lotus rhizome insects

Process	Lotus rhizome aphid	The big psychid of lotus rhizome	Lotus rhizome thorn moth
				Process 1	38.64±2.61cd	45.17±1.95c	55.26±3.24c
Process 2	41.29±0.86bc	37.22±4.60d	49.03±3.07d
				Process 3	36.85±1.15d	42.04±2.81cd	34.17±0.99f
Process 4	0.94±0.27f	1.07±0.40f	1.40±0.16g
				Process 5	95.38±5.31a	96.13±1.39a	94.83±3.92a
CK1	1.37±0.34ef	0.71±0.27f	1.61±0.27g
				Process 6	45.62±4.27b	47.35±1.18bc	61.82±4.50b
Process 7	39.74±0.82cd	51.62±6.34b	54.28±2.94c
				Process 8	40.83±3.07c	29.71±2.27e	43.19±1.43e
Process 9	2.61±0.30e	1.38±0.31f	0.83±0.31g
				Process 10	98.53±2.19a	95.27±4.08a	96.31±6.83a
CK2	1.60±0.41ef	0.94±0.25f	1.17±0.19g

Note: lowercase is 0.05 level, the different letter representation significant difference of same row, lower same.

Process 1-3,5,6-8,10 the big psychid mortality ratio of lotus rhizome be significantly higher than other process with CK1, CK2, the big psychid mortality ratio of process 5, process 10 is significantly higher than other process and CK1, CK2, and the big psychid mortality ratio of process 4,9 and CK1, CK2 are without significant difference (table 1). More than illustrating, the big psychid mortality ratio of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass is all significantly higher than people's urine and CK1, CK2, but significantly lower than the process 5,10 of chemical pesticide Rogor; Potted plant people's urine with field test processes the big psychid mortality ratio with CK without significant difference.

Process 1-3,5,6-8,10 lotus rhizome thorn moth mortality ratio be significantly higher than other process with CK1, CK2, the thorn moth mortality ratio of process 5, process 10 is significantly higher than other process and CK1, CK2, and the thorn moth mortality ratio of process 4, process 9 and CK1, CK2 are without significant difference (table 1). More than illustrating, the thorn moth mortality ratio of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass is all significantly higher than people's urine and CK1, CK2, but significantly lower than process 5, the process 10 of chemical pesticide Rogor; Potted plant people's urine with field test processes the thorn moth mortality ratio with CK without significant difference.

All being shown by above single component field experiment and pot experiment, the mortality ratio of three kinds of main lotus rhizome insects of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass is all significantly higher than the process of people's urine and CK1, CK2, but significantly processes lower than chemical pesticide Rogor. The mortality ratio of CK1, CK2 of the process of people's urine and water spray is without significant difference.

Compatibility mechanism

Compatibility mechanism mainly qualitative and quantitative measure each ingredients to kill 3 kinds of organic lotus rhizome primary pests composite effect test.

Materials and methods

2.1.1 test materials

Test is selected and organic lotus rhizome is produced serious 3 kind primary pest of causing harm: lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome thorn moth.Test ingredients be fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh, people urinate four components.

Test method

The preparation of test materials: fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh root system self-sow and become, do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact. During preparation first by fresh for Coriaria sinica, building plum grass fresh and fresh of Coriaria sinica on earth, water shake off, do not need washing. Thereafter, the mass weight ratio that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine are pressed mass weight and are mixed even frequently, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, plastic bag sealing, to preserve the vegetable insecticide of 3 years as test sterilant stoste. During field test, the thin water layer that field stays water 2～5cm dark, each community insect protected net is separated. Sterilant amount, with filtrate 25～35kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning, ensure in 15h after spraying and do not rain, also manually do not spray. Contrast chemical pesticide Rogor, by the 2000 times of liquid sprayings of 40% Rogor, every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h, ensures in 15h after spraying and does not rain, also manually do not spray. Test effect is mainly with its 3 kinds of primary pests: the per-cent of lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome aphid mortality ratio counts and statistical study. Carry out when the larva of spraying time before 3 ages or nymph.

Field test: test arranges 4 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 10m²Field area, respectively processes random alignment. Each process is numbered process 1-4 and CK1.

Pot experiment: test arranges 4 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 1 basin, respectively processes random alignment. Each process is numbered process 5-8 and CK2.

Each disposition is as follows:

Process fresh of 1 Coriaria sinica, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 2.5:1.0:1.5:3.5. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 2 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 3 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.5:2.0:2.5:4.5. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 4, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK1) water spray, viewing test effect after 24h. Process fresh of 5 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 2.5:1.0:1.5:3.5.25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 6 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 7 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.5:2.0:2.5:4.5. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 8, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK2) water spray, viewing test effect after 24h.

Statistical study

Testing data is adopted SAS(6.12) carry out statistical study. Test-results shows (table 2), the lotus rhizome aphid mortality ratio of each process is significantly higher than CK1, CK2, the aphid mortality ratio of process 2-4 is significantly higher than process 1, the aphid mortality ratio of process 6,8 is significantly higher than process 5, the aphid mortality ratio of process 2-3 and process 4 are without significant difference, and the aphid mortality ratio of process 6-7 and process 8 are also without significant difference. More than illustrating, the mass weight ratio that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment are remarkable to the killing action of aphid, and use chemical pesticide Rogor, spray by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect after 24h is substantially identical. Wherein, the mass weight that the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is significantly better than fresh of Coriaria sinica than field and the pot experiment effect for 3.0:1.5:2.0:4.0, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than the test effect for 2.5:1.0:1.5:3.5, but to urinate the mass weight of Four composition substantially more identical than the effect for 3.5:2.0:2.5:4.5 with fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people. Considering from economic benefit in conjunction with insecticidal effect, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is the better ingredients killing aphid than the compatibility for 3.0:1.5:2.0:4.0.

Table 2. compatibility mechanismThe mortality ratio (unit: %) of three kinds of main lotus rhizome insects

Process	Lotus rhizome aphid	The big psychid of lotus rhizome	Lotus rhizome thorn moth
				Process 1	86.64±2.64c	96.62±4.23ab	94.27±0.84b
Process 2	93.08±4.08b	98.30±2.94a	96.61±4.25ab
				Process 3	96.24±1.83ab	94.52±3.16b	97.82±3.49ab
Process 4	99.60±1.61a	97.43±2.49a	98.57±2.61a
				CK1	1.43±0.39d	2.61±0.84c	0.91±0.34c
Process 5	92.64±1.50b	93.48±2.27b	93.04±2.93b
				Process 6	98.27±2.19a	97.03±0.94a	98.26±0.88a
Process 7	97.34±2.71ab	98.46±1.60a	97.15±4.81ab
				Process 8	98.05±4.05a	96.17±3.59ab	99.43±3.47a
CK2	2.19±0.88d	3.16±0.73c	1.27±0.28c

The big psychid mortality ratio of lotus rhizome of each process is significantly higher than CK1, CK2, process 2,4, the big psychid mortality ratio of 6-7 be significantly higher than process 3,5, process 1-2,4, the big psychid mortality ratio of 6-8 is without significant difference. More than illustrating, the mass weight ratio that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment are remarkable to the killing action of big psychid, and use chemical pesticide Rogor, spray by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect after 24h is substantially identical.Wherein, the mass weight that the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is significantly better than fresh of Coriaria sinica than the pot experiment effect for 3.0:1.5:2.0:4.0, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than the test effect for 2.5:1.0:1.5:3.5, but to urinate the mass weight of Four composition substantially more identical than the effect for 3.5:2.0:2.5:4.5 with fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people. Considering from economic benefit in conjunction with insecticidal effect, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is the better ingredients killing big psychid than the compatibility for 3.0:1.5:2.0:4.0.

The lotus rhizome thorn moth mortality ratio of each process is significantly higher than CK1, CK2, and the thorn moth mortality ratio of process 4,6,8 is significantly higher than process 1,5, and the thorn moth mortality ratio of process 2-4,6-8 is without significant difference. More than illustrating, the mass weight ratio that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5, presses 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment are remarkable to the killing action of thorn moth, and use chemical pesticide Rogor, spray by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect after 24h is substantially identical. Wherein, the mass weight that the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is significantly better than fresh of Coriaria sinica than the pot experiment effect for 3.0:1.5:2.0:4.0, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition than the test effect for 2.5:1.0:1.5:3.5, but the mass weight urinating Four composition with fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people than the field of the compatibility for 3.5:2.0:2.5:4.5 and pot experiment effect without significant difference. Considering from economic benefit in conjunction with insecticidal effect, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is the better ingredients killing thorn moth than the compatibility for 3.0:1.5:2.0:4.0.

More than testing and also show, the mortality ratio (table 2) of three kinds of main lotus rhizome insects that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition compatibility mechanism is significantly higher than fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate the mortality ratio (table 1) that Four composition single dose tests three kinds of main lotus rhizome insects. Thus showing, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition compatibility and have good synergistic function.

Dosetest

Dosetest mainly qualitative and quantitative measure each using dosage to the effect test killing 3 kinds of organic lotus rhizome primary pests.

Materials and methods

3.1.1 test materials

Test is selected and organic lotus rhizome is produced serious 3 kind primary pest of causing harm: lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome thorn moth. Test ingredients be fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh, people urinate four components.

Test method

The preparation of test materials: fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh root system self-sow and become, do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact. During preparation first by fresh for Coriaria sinica, building plum grass fresh and fresh of Coriaria sinica on earth, water shake off, do not need washing. Thereafter, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine are pressed mass weight and are mixed even frequently, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, plastic bag sealing, to preserve the vegetable insecticide of 3 years as test sterilant stoste.During field test, the thin water layer that field stays water 2～5cm dark, each community insect protected net is separated. Sterilant amount, with filtrate 25～35kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning, ensure in 15h after spraying and do not rain, also manually do not spray. Contrast chemical pesticide Rogor, by the 2000 times of liquid sprayings of 40% Rogor, every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h, ensures in 15h after spraying and does not rain, also manually do not spray. Test effect is mainly with its 3 kinds of primary pests: the per-cent of lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome aphid mortality ratio counts and statistical study. Carry out when the larva of spraying time before 3 ages or nymph.

Each disposition is as follows:

Process fresh of 1 Coriaria sinica, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 2 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 3 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 35kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 4, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK1) water spray, viewing test effect after 24h. Process fresh of 5 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 6 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 7 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 35kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 8, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK2) water spray, viewing test effect after 24h.

Statistical study

Testing data is adopted SAS(6.12) carry out statistical study. Test-results shows (table 3), and the lotus rhizome aphid mortality ratio of process 1-4,5-8 is all significantly higher than CK1, CK2.The aphid mortality ratio of process 3-4,5-8 is significantly higher than process 1 and CK1, CK2, but processes between 2-4,5-8 without significant difference; Process 2,6 is obviously better than process 1. More than illustrating, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition, than being 3.0:1.5:2.0:4.0, presses 25-35kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and pot experiment effect are remarkable to the killing action of aphid, and use chemical pesticide Rogor, spray by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect observed after 24h is without significant difference. Wherein, compatibility liquid stoste presses 30kg.667m^-2The field test effect of dosage is obviously better than compatibility liquid stoste by 25kg.667m^-2The test effect of dosage, but press 35kg.667m with compatibility liquid stoste^-2The field of dosage and pot experiment effect are without significant difference. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stoste presses 30kg.667m^-2Dosage be the better dosage killing aphid.

Table 3. dosetestThe mortality ratio (unit: %) of three kinds of main lotus rhizome insects

Process	Lotus rhizome aphid	The big psychid of lotus rhizome	Lotus rhizome thorn moth
				Process 1	93.08±3.51b	98.30±0.94a	96.61±2.43b
Process 2	96.42±2.47ab	99.07±3.28a	98.43±1.92ab
				Process 3	98.05±4.16a	99.80±1.92a	99.25±4.08a
Process 4	99.60±1.84a	97.43±4.63ab	98.57±2.64ab
				CK1	1.43±0.31c	2.61±0.41c	0.91±0.10c
Process 5	98.27±2.79a	97.03±2.39ab	98.26±3.67ab
				Process 6	98.24±3.42a	99.26±4.11a	99.40±4.53a
Process 7	99.16±1.67a	99.60±3.40a	99.71±2.28a
				Process 8	98.05±0.91a	96.17±1.16b	99.43±1.90a
CK2	2.19±1.13c	3.16±0.71c	1.27±0.24c

The big psychid mortality ratio of lotus rhizome of process 1-4,5-8 is all significantly higher than CK1, CK2. Without significant difference between process 1-4, process 6-7 is significantly higher than process 8(table 3). More than illustrating, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition, than being 3.0:1.5:2.0:4.0, presses 25-35kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment are remarkable to the killing action of the big psychid of lotus rhizome, spray with by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect observed after 24h is without significant difference. Wherein, compatibility liquid stoste presses 30kg.667m^-2The pot experiment successful of dosage is better than compatibility liquid stoste by 25kg.667m^-2The pot experiment effect of dosage, but press 35kg.667m with compatibility liquid stoste^-2The field of dosage and pot test effect are without significant difference. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stoste presses 30kg.667m^-2Dosage be the better dosage killing the big psychid of lotus rhizome.

The lotus rhizome thorn moth mortality ratio of process 1-4,5-8 is all significantly higher than CK1, CK2. Without significant difference between process 2-4, without significant difference (table 3) between process 5-8; Process 2,6 is obviously better than process 1,5. More than illustrating, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition, than being 3.0:1.5:2.0:4.0, presses 25-35kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, the killing action that lotus rhizome is stung moth by field after 24h and the effect of pot experiment is remarkable, sprays with by 40% Rogor, 2000 times of liquid, every 667m²The lotus rhizome field dosage of diluent 70kg, the test effect observed after 24h is without significant difference. Wherein, compatibility liquid stoste presses 30kg.667m^-2The field of dosage and pot experiment effect are significantly better than compatibility liquid stoste by 25kg.667m^-2The test effect of dosage, but press 35kg.667m with compatibility liquid stoste^-2The field of dosage and pot test effect are without significant difference. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stoste presses 30kg.667m^-2Dosage be kill lotus rhizome thorn moth better dosage.

More than test shows, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition, than being 3.0:1.5:2.0:4.0, presses 30kg.667m by this compatibility liquid stoste^-2The effect that dosage 1.0:2.0 dilutes the field of spraying and pot experiment kills 3 kinds of lotus rhizome primary pests be significantly better than 25kg.667m^-2The effect of dosage spraying, but and 35kg.667m^-2The effect of dosage spraying is substantially identical. Consider from economic benefit in conjunction with insecticidal effect, 30kg.667m^-2Dosage spraying is better dosage.

More than test and also show, the mortality ratio (table 1) that the mortality ratio (table 2-3) of insecticidal effect that is 3 kind lotus rhizome primary pest that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition compatibility dosetest is significantly higher than fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate the test of Four composition single dose. Thus showing, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition compatibility and have good synergistic function.

Auxiliary agent is tested

Auxiliary agent is tested mainly qualitative and quantitative and is measured the synergistic function that whole ingredients is killed 3 kinds of organic lotus rhizome primary pests by auxiliary agent.

Materials and methods

4.1.1 test materials

Test is selected and organic lotus rhizome is produced serious 3 kind primary pest of causing harm: lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome thorn moth. Test ingredients be fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh, people urinate four components. Wherein fresh of building plum grass is auxiliary agent.

Test method

The preparation of test materials: fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh root system self-sow and become, do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact. During preparation first by fresh for Coriaria sinica, building plum grass fresh and fresh of Coriaria sinica on earth, water shake off, do not need washing. Thereafter, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine are pressed mass weight and are mixed even frequently, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, plastic bag sealing, to preserve the vegetable insecticide of 3 years as test sterilant stoste. During field test, the thin water layer that field stays water 2～5cm dark, each community insect protected net is separated. Sterilant amount, uses filtrate 30kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning, ensure in 15h after spraying and do not rain, also manually do not spray. Contrast chemical pesticide Rogor, by the 2000 times of liquid sprayings of 40% Rogor, every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h, ensures in 15h after spraying and does not rain, also manually do not spray. Test effect is mainly with its 3 kinds of primary pests: the per-cent of lotus rhizome aphid, the big psychid of lotus rhizome and lotus rhizome aphid mortality ratio counts and statistical study. Carry out when the larva of spraying time before 3 ages or nymph.

Field test: test arranges 3 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 10m²Field area, respectively processes random alignment.Each process is numbered process 1-3 and CK1.

Pot experiment: test arranges 3 process, 1 comparison, and each processes 3 repetitions (comprising CK), and each repeats 1 basin, respectively processes random alignment. Each process is numbered process 4-6 and CK2.

Each disposition is as follows:

Process fresh of 1 Coriaria sinica, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 2 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, people urinate three components is than being 3.0:1.5:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 3, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK1) water spray, viewing test effect after 24h. Process fresh of 4 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Process fresh of 5 Coriaria sinicas, mass weight that the fresh radical center of trypterygine, people urinate three components is than being 3.0:1.5:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. Viewing test effect after 24h. Chemical pesticide Rogor is used in process 6, by the 2000 times of liquid sprayings of 40% Rogor, and every 667m²The lotus rhizome field dosage of diluent 70kg. Viewing test effect after 24h. Comparison (CK2) water spray, viewing test effect after 24h.

Statistical study

Testing data is adopted SAS(6.12) carry out statistical study. Test-results shows (table 4), and the lotus rhizome aphid mortality ratio of process 2,5 is significantly lower than process 1,3,4,6, but is significantly higher than CK1, CK2. More than illustrating, the mass weight urinating three components without fresh of the compatibility Coriaria sinica of auxiliary agent, the fresh radical center of trypterygine, people, than being 3.0:1.5:4.0, presses 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, the effect of the field after 24h and pot experiment to aphid kill mortality ratio significantly lower than the mass weight having fresh of the compatibility Coriaria sinica of auxiliary agent, the fresh radical center of trypterygine, fresh of building plum grass, people to urinate Four composition than being 3.0:1.5:2.0:4.0, press 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the mortality ratio of pot experiment; Also significantly spray every 667m by 40% Rogor, 2000 times of liquid lower than with chemical pesticide²Lotus rhizome the field field after the dosage 24h of diluent 70kg and pot experiment mortality ratio.

Table 4. auxiliary agent is testedThe mortality ratio (unit: %) of three kinds of main lotus rhizome insects

Process	Lotus rhizome aphid	The big psychid of lotus rhizome	Lotus rhizome thorn moth
				Process 1	96.42±2.49b	99.07±4.71a	98.43±3.91a
Process 2	84.61±0.81c	68.25±2.44d	80.42±1.81b
				Process 3	99.60±4.26a	97.43±1.93ab	98.57±4.67a
CK1	1.83±0.31d	2.61±1.18e	0.97±0.22c
				Process 4	97.24±1.82ab	99.26±5.27a	99.40±5.49a
Process 5	82.09±5.17c	71.49±3.60c	82.60±2.97b
				Process 6	98.05±2.46a	96.17±1.19b	99.43±4.10a
CK2	2.19±1.20d	3.16±1.72e	1.27±0.31c

The big psychid mortality ratio of lotus rhizome of process 2,5 is significantly lower than process 1,3,4,6, but is significantly higher than CK1, CK2(table 4). More than illustrating, the mass weight urinating three components without fresh of the compatibility Coriaria sinica of auxiliary agent, the fresh radical center of trypterygine, people, than being 3.0:1.5:4.0, presses 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment to the mortality ratio of big psychid significantly lower than the compatibility having auxiliary agent: fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate the mass weight of Four composition than being 3.0:1.5:2.0:4.0, press 30kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, big psychid is killed mortality ratio by field after 24h and the effect of pot experiment;Also significantly spray every 667m by 40% Rogor, 2000 times of liquid lower than with chemical pesticide²Lotus rhizome the field field after the dosage 24h of diluent 70kg and pot experiment mortality ratio.

The lotus rhizome thorn moth mortality ratio of process 2,5 is significantly lower than process 1,3,4,6, but is significantly higher than CK1, CK2(table 4). More than illustrate, the compatibility without auxiliary agent: fresh of Coriaria sinica, the fresh radical center of trypterygine, people urinate the mass weight of three components than being 3.0:1.5:4.0, press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, thorn moth is killed mortality ratio significantly lower than the compatibility having auxiliary agent by field after 24h and the effect of pot experiment: fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate the mass weight of Four composition than being 3.0:1.5:2.0:4.0, press 25kg.667m by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying, field after 24h and the effect of pot experiment kill mortality ratio to what sting moth; Also significantly spray every 667m by 40% Rogor, 2000 times of liquid lower than with chemical pesticide²Lotus rhizome the field field after the dosage 24h of diluent 70kg and pot experiment mortality ratio.

More than test shows (table 4), and adding assistant is remarkable with the difference on effect of not adding assistant, and the effect of adding assistant is significantly better than the effect of not adding assistant, and its effect of adding assistant does not significantly reduce. Illustrate that fresh of auxiliary agent building plum grass has significant collaborative and synergism in this compatibility.

Test also shows (table 1-4), and the effect of ingredients is the 1.5-2 doubly left and right of each single dose effect. So, the effect after compatibility is not the addition of simple each single dose effect. Being tested to compatibility mechanism from single dose and test (table 1-4) to auxiliary agent again, result shows, the mass weight that fresh of compatibility Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition than the compatibility liquid stoste for 3.0:1.5:2.0:4.0 by 30kg.667m^-2Dosage 1.0:2.0 dilute spraying ingredients, from economic benefit in conjunction with insecticidal effect consider be better selection. Test shows, the compatibility agent urinating Four composition with fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people sprays the main component Coriaria sinica alkali of fresh of non-detection Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass in the commodity lotus rhizome of rear 30d, robinin, wilforine etc.

Coriaria sinica (CoriarianepalensisWall.) belonging to Seed of Chinese Soapberry order, Coriaria sinica section shrub, its leaf is to life, and papery is to Bao Gezhi, and oval, raceme is born on biennial branch, petal meat, keel-shaped. The first leaf of male inflorescence is open, spends more intensive; Sepal is avette, and edge is translucent, top tool tasselled shape serration; Extend during filigree flower; There is sterile gynoecium. Female inflorescence and leaf go out together, red-purple. Berry shape achene, becomes atropurpureus by redness, the month at florescence 3-4 time ripe, fruit month phase 5-6. It is distributed in the ground such as Yunnan, Guizhou, Sichuan, Hubei, Hunan, Shaanxi, Gansu. Fruit can carry alcohol. Seed oil expression can be painted and ink. Stem leaf can carry tannin extract. Complete stool is containing Coriaria sinica alkali, and hydroxyl Coriaria sinica toxin etc., its toxic ingredient is Coriamyrtin, tells butyrolactone etc.

Trypterygine (TripterygiumwilfordiiHook.f.), winged euonymus section, tripterygium plant, liana shrub, main product is in China, Korea, Japan, and China is mainly distributed in various places and southwest on the south the Yangtze valley, main product in Fujian, Zhejiang, Hubei, Hunan, Anhui, the ground such as Henan. In being born in the shade many wet hillside, mountain valley, small stream limit thicket. The hillside that happiness is comparatively shady and cool, the sandy soil deep with slant acidity, soil layer or the most suitable growth of yellow soil. Bitter, pungent, cool in nature, poison greatly. Thunder God Calamus liana shrub, high 1-3 rice, sprig red-brown, has the thin rib of 4-6 root, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, rectangular ellipse or avette, long 4-7.5 centimetre, wide 3-4 centimetre, the anxious point of tip or short gradually point, the wealthy wedge shape in base portion or circle, there is serrulation at edge, 4-7 pair, side arteries and veins, slightly goes up curved after reaching leaf margin; The long 5-8 millimeter of petiole, close by rust hair. Circular cone cyme is narrower, long 5-7 centimetre, wide 3-4 centimetre, usually has 3-5 branch. The oval shape of samara, long 1-1.5 centimetre, diameter 1-1.2 centimetre, seed buttress shaft shape, reaches 10 millimeters. Trypterygine bitter, pungent, cool in nature, poison greatly, has clearing heat and detoxicating, dispelling wind and removing obstruction in the collateral, relaxes the muscles and stimulate the blood circulation, swelling and pain relieving, kills the effects such as worm hemostasis, return liver, kidney channel, and effect is dispelled rheumatism, removed obstruction in channels to relieve pain, swelling and pain relieving, solution poisoning worm. The traditional Chinese medical science is used for the treatment of leprosy reaction, rheumatoid arthritis, pulmonary tuberculosis and other chronic lung diseases etc. Trypterygine has the effects such as antitumor, anti-inflammation, but it is very toxic, so trypterygine is restricted in application medically always. Tripterygium root mainly contains wilforine, Stem of Oriental Bittersweet benzyl acid amides, Triptolide etc.

Lou Meicao (BuddlejalindleyanaFortune) Loganiaceae shrub, stem skin brown; Sprig tool four rib, slightly narrow wing on rib; Children branch, blade below, petiole, inflorescence, bract and bractlet all close by starlike down and glandular hairs. Leaf is to life, and the leaf on rudiment branch is alternate or closely verticillate, blade film matter, and avette, oval to oval shape lanceolar, base portion often has place to deposit calyx; Seed is filbert, little, aptery. The month at florescence 4-10, really April in August phase to next year. In originating in roadside, mountain region, the river bank thickets such as Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan or border. Complete stool is containing multiple flavonoids such as Herba Linariae Vulgaris glucoside, robinins.

Embodiment

Hereinafter enforcement mode and embodiment are intended to illustrate further the present invention, instead of limitation of the present invention.

Embodiment 1:

The preparation of material: fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh root system self-sow and become, do not adopt the mountain area of chemical fertilizer, chemical pesticide. Otherwise, by the quality of the organic lotus rhizome of impact. During preparation first by fresh for Coriaria sinica, building plum grass fresh and fresh of Coriaria sinica on earth, water shake off, do not need washing. Thereafter, the mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine press mass weight than mixed even, the big plastic tank of 25～50L adds does not need sealing between lid immersion 20～40d(soak period), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic lotus rhizome vegetable insecticide, and plastic bag sealing, can preserve 2～3 years.

Select duration of service to carry out spray medicine when the larva before organic lotus rhizome 3 kinds primary pest 3 age or nymph, it is possible to carry out early prevention. During use, the thin water layer that field stays water 2～5cm dark, uses filtrate 25kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning or at dusk, do not rain in guarantee 15～20h after spraying, also manually do not spray, if having rained in 15～20h or having carried out artificial sprinkling irrigation, need to again spray. Carrying out when the larva of spraying time before 3 ages or nymph, droplet is as far as possible fine, not repeat spraying, is limited not form the globule on plant, and safe defence strictly to be carried out human body defence and field operation by chemical pesticide working specification.After spray medicine, in 30d, field can not carry out the mixed battalion of breeding.

The mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 25kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. After 24h, the insecticidal effect in organic lotus rhizome field is: lotus rhizome aphid mortality ratio 93.08%, the big psychid mortality ratio 98.30% of lotus rhizome, lotus rhizome thorn moth mortality ratio 96.61%, only 6.52% ,-0.87%, 1.96% is differed respectively, substantially close to the insecticidal effect of chemical pesticide Rogor with the insecticidal effect of chemical pesticide Rogor.

Embodiment 2:

Select duration of service to carry out spray medicine when the larva before organic lotus rhizome 3 kinds primary pest 3 age or nymph, it is possible to carry out early prevention. During use, the thin water layer that field stays water 2～5cm dark, uses filtrate 30kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning or at dusk, do not rain in guarantee 15～20h after spraying, also manually do not spray, if having rained in 15～20h or having carried out artificial sprinkling irrigation, need to again spray. Carrying out when the larva of spraying time before 3 ages or nymph, droplet is as far as possible fine, not repeat spraying, is limited not form the globule on plant, and safe defence strictly to be carried out human body defence and field operation by chemical pesticide working specification. After spray medicine, in 30d, field can not carry out the mixed battalion of breeding.

The mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 30kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. After 24h, the insecticidal effect in organic lotus rhizome field is: lotus rhizome aphid mortality ratio 96.42%, the big psychid mortality ratio 99.07% of lotus rhizome, lotus rhizome thorn moth mortality ratio 98.43%, only 3.18% ,-1.64%, 0.14% is differed respectively with the insecticidal effect of chemical pesticide Rogor, substantially identical with the insecticidal effect of chemical pesticide Rogor.

Embodiment 3:

Select duration of service to carry out spray medicine when the larva before organic lotus rhizome 3 kinds primary pest 3 age or nymph, it is possible to carry out early prevention. During use, the thin water layer that field stays water 2～5cm dark, uses filtrate 35kg.667m^-2By 1.0:2.0 dilution spraying. Select to spray before at 10 in fine day morning or at dusk, do not rain in guarantee 15～20h after spraying, also manually do not spray, if having rained in 15～20h or having carried out artificial sprinkling irrigation, need to again spray. Carrying out when the larva of spraying time before 3 ages or nymph, droplet is as far as possible fine, not repeat spraying, is limited not form the globule on plant, and safe defence strictly to be carried out human body defence and field operation by chemical pesticide working specification. After spray medicine, in 30d, field can not carry out the mixed battalion of breeding.

The mass weight that fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people urinate Four composition is than being 3.0:1.5:2.0:4.0. 35kg.667m is pressed by this compatibility liquid stoste^-2Dosage 1.0:2.0 dilute spraying. After 24h, the insecticidal effect in organic lotus rhizome field is: lotus rhizome aphid mortality ratio 98.05%, the big psychid mortality ratio 99.80% of lotus rhizome, lotus rhizome thorn moth mortality ratio 99.25%, only 1.55% ,-2.37% ,-0.68% is differed respectively with the insecticidal effect of chemical pesticide Rogor, substantially identical with the insecticidal effect of chemical pesticide Rogor.

Claims

1. an organic lotus rhizome vegetable insecticide and using method thereof, it is characterised in that, described organic lotus rhizome vegetable insecticide is urinated brewed and become by fresh of Coriaria sinica, the fresh radical center of trypterygine, building plum grass fresh compatibility people.

2. the organic lotus rhizome vegetable insecticide of one according to claim 1, it is characterized in that, the compatibility weight mass ratio of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of auxiliary agent building plum grass and brewed medium people urine is 2.5～3.5:1.0～2.0:1.5～2.5:3.5～4.5.

3. a kind of organic lotus rhizome vegetable insecticide according to claim 1 and 2, it is characterised in that, the preferred weight mass ratio of fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass and people's urine is 3.0:1.5:2.0:4.0.

4. organic lotus rhizome vegetable insecticide according to claims 1 to 3, it is characterized in that, fresh of Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass are all by the weight Mass Calculation of fresh material, and people's urine is by the weight Mass Calculation of 1～30d, wherein fresh of Coriaria sinica is auxiliary agent, and people's urine is brewed medium.

5. according to the organic lotus rhizome vegetable insecticide described in claim 2 to 4, it is characterized in that, fresh of material Coriaria sinica, the fresh radical center of trypterygine, fresh of building plum grass, people's urine all should be selected from free of contamination areas of envrionment conditions such as soil, water, air, and soil, water body and fertilizer etc. also want heavy metal free to pollute.

6. according to the organic lotus rhizome vegetable insecticide described in claim 2 to 4, it is characterized in that, during use, the thin water layer that field need to stay water 2～5cm dark, before being typically chosen at 10 in fine day morning or sprayed at dusk, to be ensured after spraying and not rain in 15～20h or manually spray, if having rained in 15～20h or having carried out artificial sprinkling irrigation, need to again spray.

7. using method according to claim 6, is characterized in that, selects the larva before 3 ages or nymph to carry out spray medicine, by every 667m²The dosage of brewed dose of paddy field area 25～35kg presses 1.0:2.0 dilution spraying, droplet is as far as possible fine, not repeat spraying, is limited not form the globule on plant, and the lotus rhizome aphid of organic lotus rhizome Tanaka, the big psychid of lotus rhizome and lotus rhizome thorn moth three kinds of primary pests can be carried out effective killing action.