CN104782674A - Aphid removal agent for organic vegetables and using method of aphid removal agent - Google Patents

Aphid removal agent for organic vegetables and using method of aphid removal agent Download PDF

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CN104782674A
CN104782674A CN201510208417.1A CN201510208417A CN104782674A CN 104782674 A CN104782674 A CN 104782674A CN 201510208417 A CN201510208417 A CN 201510208417A CN 104782674 A CN104782674 A CN 104782674A
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aphid
tea
oil
cake
urine
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CN104782674B (en
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杨洁
李红飞
郭兆武
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Changsha University of Science and Technology
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Changsha University of Science and Technology
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Abstract

The invention discloses an aphid removal agent for organic vegetables and a using method of the aphid removal agent. The removal agent is processed by thunder god vines and roots, stems of red-knees herb, human urine and tea-oil tree cakes which are combined, wherein the aphid removal agent is a combined mixture with the mass ratio of thunder god vines and roots, stems of red-knees herb, human urine and tea-oil tree cakes being (3.0-4.0): (1.0-2.0): (6.0-8.0): (0.5-1.0), preferably 3.5: 1.5: 7.5: 0.75; the thunder god vines and roots and stems of red-knees herb are calculated based on fresh weight, the tea-oil cakes are calculated based on weight of dry cakes which are not mildewed and absorbs moisture for 1-30 days, and the human urine is calculated based on the weight of pure urine which is not doped with excrements and the like and stored for 1-30 days; the human urine is a processing medium, the oil-tea cakes are auxiliary agents, and the combined processing liquid is a raw liquid. The combined agent is remarkable in preventing and treating effect on aphids of organic vegetables and has a synergistic effect. When the aphid removal agent is used for preventing and treating insect pests, the aphid removal agent is sprayed with 10 times of a liquid based on 5-15kg of the raw liquid per 667m<2>, so that the aphids can be quickly killed to inhibit spread of the aphids. The aphid removal agent further can be used for supplementing nutrients such as nitrogen so as to accelerate recovery of pest strains. The aphid removal agent in production is convenient to use, simple to operate, low in cost and remarkable in preventing and treating effect.

Description

A kind of organic vegetable control of aphids agent and using method thereof
Technical field
The invention belongs to agriculture practical new technology research and development and application; The present invention relates to a kind of research and development and production application method of organic vegetable control of aphids agent.
Background technology
Vegetable aphid is commonly called as aphid, aphid etc., belongs to homoptera pest.Vegetable aphid is one of primary pest of crops, and kind is many, and generation is many, and breeding is fast, and harm is heavy.Vegetable aphid mainly contains black peach aphid (also claiming cigarette aphid), radish aphid, melon aphid (also claiming cotten aphid), cabbage aphid, bean aphid (also claiming Aphis medicaginis, aphis craccivora) etc., if sting to become aphid aphid and inhale host's juice, cause plant strain growth bad, when being injured serious, can leaf curl be made, branches and leaves jaundice distortion, bud is ruined, florescence shortens, and flower appearance loses lustre, growth retardation, time serious, complete stool withered death, causes total crop failure; Meanwhile, various vegetables virus disease can be propagated again, bring out vegetable disease, endanger larger.Vegetable aphid is mainly intensive at vegetable seedling, tender leaf, stem and subaerial blade back with adult, nymph, and thorn inhales juice.All have generation throughout the year, generally temperature at about 29 DEG C time breeding the fastest.At present, physical control, chemical control, biological control etc. are mainly contained to the prevention and controls of vegetable aphid.Crocus lath is utilized to coat the physical control of tack coat trapping aphid, the biological control advantage utilizing the anti-aphid of natural enemy is pollution-free, but its control efficiency is not thorough, just a kind of suppression strictly speaking, to the vegetables of the types such as some melon dish, leaf vegetabless, to its any damage, its attractive in appearance and quality all can be had a strong impact on, concerning these vegetables, any damage can not be had.So physical control and biological control have its limitation, namely have larger defect, and cost is higher, this is also that it is difficult to one of reason extensively promoted.At present, the two supplementary means of just preventing and treating.Chemical control resembles and uses more general synthetic organic pesticide Acetamiprid, Imidacloprid etc. at present, though control is relatively more thorough, its toxicity is large, and residual harm is serious, is to forbid to use during organic vegetable is produced.This organic vegetable control of aphids agent overcomes above defect, both pollution-free, has again good control efficiency, and cost is also very low, is a kind of well organic vegetable control of aphids agent.Insect pest uses before occurring, and can prevent to occur; Use after generation, comparatively fast can kill aphid, stop insect pest to spread, plant restore normal growth soon.This organic vegetable control of aphids agent is the achievement through the application of R & D and manufacture for many years, and it both can be prevented, can control again, and easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and this is other about medicament or measure institute cannot be than.
Summary of the invention
Object of the present invention aims to provide a kind of organic vegetable control of aphids agent, and easy to use, simple to operate, cost is low, and control efficiency is remarkable.Another object of the present invention aims to provide the preferably using method of above-mentioned organic vegetable control of aphids agent.The object of the invention is to realize in the following manner:
Control agent of the present invention is tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people urinate composition, wherein Oil-tea-cake is mainly used as auxiliary agent, play a part to promote its control efficiency, can add according to production application, people urinates main as brewed medium, also have effect of foliar application, accelerate the recovery that the normal growth after deinsectization is grown.Inventor studies discovery, adds auxiliary agent Oil-tea-cake and can play good synergistic effect.Need it is emphasized that, the tripterygium root of this organic vegetable control of aphids agent, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, thunder godvine, polygonum flaccidum, tea oil tree system self-sow form, and do not use the mountain area of chemical fertilizer, chemical pesticide.Otherwise, will the quality of organic vegetable be affected.
The preparation method of this organic vegetable control of aphids agent is: material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, soil, water body and fertilizer etc. also want heavy metal free to pollute, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that do not adulterate water, ight soil etc. deposit 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that 10cm is long, with hammer, Oil-tea-cake be pounded the fritter of 5 × 5cm size, be soaked in proportion in urine, after mixing, add a cover immersion 15-30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by do not adulterate water, ight soil etc.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15-30d.Add a cover and soak after 15-30d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, can preserve 1-6 month.This organic vegetable control of aphids agent is used before insect pest occurs, and prevention is by every 667m 2the 10 times of liquid sprayings of 5 ~ 10kg stoste; Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 10 ~ 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.This control agent is produced upper easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and the shelf-life is longer, packs with plastic bag sealing, can carry out factorial praluction, forms product.
The most preferred mass ratio of above Four composition tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is 3.5:1.5:7.5:0.75, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by do not adulterate water, ight soil etc.Add a cover after mixing and soak 15-30d.Add a cover and soak after 15-30d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, can preserve 1-6 month.This organic vegetable control of aphids agent is used before insect pest occurs, and prevention is by every 667m 2the 10 times of liquid sprayings of 5 ~ 10kg stoste; Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 10 ~ 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate the recovery of plant injury, growth promoting effects.This control agent is produced upper easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and the shelf-life is longer, packs with plastic bag sealing stoste (i.e. mother liquor), can carry out factorial praluction, forms product.
The present invention selects control agent tripterygium root, polygonum flaccidum stem, people's urine and auxiliary agent Oil-tea-cake, and have employed the compatibility of suitable concentration, be mixed with commercial control agent, it both can be prevented, can control again, and it has significant preventive and therapeutic effect to organic vegetable aphid.Using the control of aphids of this organic vegetable is its comparatively one of effective measures.This control agent is easy to use, simple to operate, and cost is low, and control efficiency is remarkable, and this is other about medicament or measure institute cannot be than.This organic vegetable control of aphids agent prescription is tripterygium root, polygonum flaccidum stem, people's urine and auxiliary agent Oil-tea-cake, and brewed by the ratio mixing of above-mentioned mass ratio, mother liquor plastic bag sealing is packed, and shady and cool dry lucifuge place preserves, 6 months shelf-lifves.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.It is below the comparative test of its regulating effect.Organic vegetable control of aphids agent of the present invention has no the report of like product at present, and the present invention is the result of the test that inventor draws through experimental study for many years and production practices, and is verified in the production of long period, and effect is better.And formula of the present invention also possesses skills, content is higher, and formulation is more stable, abundant raw material source, easy and simple to handle, as being developed to commodity, and low price.Easy to use aborning, control efficiency is remarkable, and use cost is low, is a kind of good organic vegetable control of aphids agent.Organic vegetable control of aphids agent prescription can carry out factorial praluction, and its exploitation not only commercially will increase a kind of novel organic vegetable control of aphids agent, also provide an approach solved by the problem for current organic vegetable control of aphids poor effect.
Using method of the present invention is: this organic vegetable control of aphids agent is used before insect pest occurs, and prevention is by every 667m 25 ~ 10kg stoste, 10 times of liquid are sprayed; Use, by every 667m after insect pest occurs 210 ~ 15kg stoste, 10 times of liquid are sprayed, and comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.
Brewing method of the present invention is: first removed by the earth on tripterygium root, polygonum flaccidum stem during preparation, does not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that 10cm is long, with hammer, Oil-tea-cake be pounded the fritter of 5 × 5cm size, be soaked in proportion in urine, after mixing, add a cover immersion 15-30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.The compatibility mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15-30d.Add a cover and soak after 15-30d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, can preserve 1-6 month.Use in rear 24h and can not sprinkle water or apply fertilizer, conventional fertilizer water management after 24h, rained if used within rear 24h, then needed after rain again to process.Test shows, after using 15d, detects vegetables, and the main functional component of its medicament does not detect, and period does not descend moderate rain or heavy rain; After using, period following moderate rain or heavy rain more than 1 time, after using 7d, its main functional component does not detect.
Be below invent artificially to realize object of the present invention, constantly select and select the comparative test of the control efficiency that the raw material of applicable organic vegetable control of aphids agent prescription of the present invention and relevant proportioning thereof are done by test.
one pack system is tested
One pack system tests the single effect test that each component of mainly qualitative determination is prevented and treated organic vegetable aphid.
materials and methods
1.1.1 test material
Test vegetable species select the most easily break out aphid Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables.Test ingredients is: tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake.
test method
The preparation of test material: material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size.The ratio that tripterygium root, polygonum flaccidum stem, Oil-tea-cake describe in next section is respectively soaked in urine, adds a cover and soak 15-30d after mixing.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.
Tripterygium root component is by following mass ratio preparation mother liquor: tripterygium root: people urine=3.5:7.5(wherein people's urine is brewed medium, and its quality is included into its mother liquor gross mass).Polygonum flaccidum stem component prepares mother liquor by following mass ratio, polygonum flaccidum stem: people urine=1.5:7.5(wherein people's urine is brewed medium, and its quality is included into its mother liquor gross mass).Oil-tea-cake component prepares mother liquor by following mass ratio, Oil-tea-cake: people urine=0.75:7.5(wherein people's urine is brewed medium, and its quality is included into its mother liquor gross mass).People urinates component and adopts the people's urine having deposited 30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 30d by do not adulterate water, ight soil etc.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be female stoste of each component single dose, preserve 1-6 month.When breaking out aphid, lid being opened, taking out each single dose stoste (people urinates component and takes out the people's urine having deposited 30d) respectively, by every 667m 25,10,10 times of liquid (referring to amount of water) sprayings of 15kg stoste 3 concentration gradients.
Test is carried out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When test effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time process.Each single dose amount of application: its mother liquor amount of application of tripterygium root component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out spray application.Its mother liquor amount of application of polygonum flaccidum stem component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out spray application.Its mother liquor amount of application of Oil-tea-cake component is by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out spray application.People urinates component by every 667m 25kg, 10kg, 15kg tri-kinds of concentration gradients carry out spray application.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.Test arranges 48 process, 4 contrasts, each process 3 repetition, each repetition 0.10m 2, random distribution.Using Percent mortality as treatment effect.
Process 1: the aphid of Chinese cabbage---tripterygium root is by every 667m 25kg uses; Process 2: the aphid of Chinese cabbage---tripterygium root is by every 667m 210kg uses; Process 3: the aphid of Chinese cabbage---tripterygium root is by every 667m 215kg uses; Process 4: the aphid of Chinese cabbage---polygonum flaccidum stem is by every 667m 25kg uses; Process 5: the aphid of Chinese cabbage---polygonum flaccidum stem is by every 667m 210kg uses; Process 6: the aphid of Chinese cabbage---polygonum flaccidum stem is by every 667m 215kg uses; Process 7: the aphid of Chinese cabbage---Oil-tea-cake is by every 667m 25kg uses; Process 8: the aphid of Chinese cabbage---Oil-tea-cake is by every 667m 210kg uses; Process 9: the aphid of Chinese cabbage---Oil-tea-cake is by every 667m 215kg uses; Process 10: the aphid of Chinese cabbage---people's urine is by every 667m 25kg uses; Process 11: the aphid of Chinese cabbage---people's urine is by every 667m 210kg uses; Process 12: the aphid of Chinese cabbage---people's urine is by every 667m 215kg uses; CK1: the contrast of Chinese cabbage, not spray pharmacy, water spray.Process 13: the aphid of cucumber---tripterygium root is by every 667m 25kg uses; Process 14: the aphid of cucumber---tripterygium root is by every 667m 210kg uses; Process 15: the aphid of cucumber---tripterygium root is by every 667m 215kg uses; Process 16: the aphid of cucumber---polygonum flaccidum stem is by every 667m 25kg uses; Process 17: the aphid of cucumber---polygonum flaccidum stem is by every 667m 210kg uses; Process 18: the aphid of cucumber---polygonum flaccidum stem is by every 667m 215kg uses; Process 19: the aphid of cucumber---Oil-tea-cake is by every 667m 25kg uses; Process 20: the aphid of cucumber---Oil-tea-cake is by every 667m 210kg uses; Process 21: the aphid of cucumber---Oil-tea-cake is by every 667m 215kg uses; Process 22: the aphid of cucumber---people's urine is by every 667m 25kg uses; Process 23: the aphid of cucumber---people's urine is by every 667m 210kg uses; Process 24: the aphid of cucumber---people's urine is by every 667m 215kg uses; CK2: the contrast of cucumber, not spray pharmacy, water spray.Process 25: the aphid of radish---tripterygium root is by every 667m 25kg uses; Process 26: the aphid of radish---tripterygium root is by every 667m 210kg uses; Process 27: the aphid of radish---tripterygium root is by every 667m 215kg uses; Process 28: the aphid of radish---polygonum flaccidum stem is by every 667m 25kg uses; Process 29: the aphid of radish---polygonum flaccidum stem is by every 667m 210kg uses; Process 30: the aphid of radish---polygonum flaccidum stem is by every 667m 215kg uses; Process 31: the aphid of radish---Oil-tea-cake is by every 667m 25kg uses; Process 32: the aphid of radish---Oil-tea-cake is by every 667m 210kg uses; Process 33: the aphid of radish---Oil-tea-cake is by every 667m 215kg uses; Process 34: the aphid of radish---people's urine is by every 667m 25kg uses; Process 35: the aphid of radish---people's urine is by every 667m 210kg uses; Process 36: the aphid of radish---people's urine is by every 667m 215kg uses; CK3: the contrast of radish, not spray pharmacy, water spray.Process 37: the aphid of fresh kidney beans---tripterygium root is by every 667m 25kg uses; Process 38: the aphid of fresh kidney beans---tripterygium root is by every 667m 210kg uses; Process 39: the aphid of fresh kidney beans---tripterygium root is by every 667m 215kg uses; Process 40: the aphid of fresh kidney beans---polygonum flaccidum stem is by every 667m 25kg uses; Process 41: the aphid of fresh kidney beans---polygonum flaccidum stem is by every 667m 210kg uses; Process 42: the aphid of fresh kidney beans---polygonum flaccidum stem is by every 667m 215kg uses; Process 43: the aphid of fresh kidney beans---Oil-tea-cake is by every 667m 25kg uses; Process 44: the aphid of fresh kidney beans---Oil-tea-cake is by every 667m 210kg uses; Process 45: the aphid of fresh kidney beans---Oil-tea-cake is by every 667m 215kg uses; Process 46: the aphid of fresh kidney beans---people's urine is by every 667m 25kg uses; Process 47: the aphid of fresh kidney beans---people's urine is by every 667m 210kg uses; Process 48: the aphid of fresh kidney beans---people's urine is by every 667m 215kg uses; CK4: the contrast of fresh kidney beans, not spray pharmacy, water spray.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.After process 24h, the effect of each process is in Table 1-2.Result of the test shows (table 1), and the aphid lethality of process 3 is significantly higher than process 1-2, illustrates that the Be very effective of aphid on higher concentration tripterygium root control Chinese cabbage is better than the tripterygium root of low concentration.Process 1-3 is significantly higher than again other process and CK1, and namely on tripterygium root control Chinese cabbage, the Be very effective of aphid is better than the control efficiency of polygonum flaccidum stem, Oil-tea-cake, people's urine.
On variable concentrations polygonum flaccidum stem control Chinese cabbage, the effect of aphid is: the aphid lethality of process 6 is significantly higher than process 4-5, illustrates that the Be very effective of aphid on higher concentration polygonum flaccidum stem control Chinese cabbage is better than the polygonum flaccidum stem of low concentration.Process 4-6 is significantly higher than again process 7-12 and CK1, and namely on polygonum flaccidum stem control Chinese cabbage, the Be very effective of aphid is better than the control efficiency of Oil-tea-cake, people's urine, but the control efficiency of too late tripterygium root.On variable concentrations Oil-tea-cake control Chinese cabbage, the effect of aphid is: the process 9 of higher concentration and the process 7-8 aphid lethality of low concentration are without significant difference, but process 8-9 is significantly higher than process 10-12 and CK1, namely on Oil-tea-cake control Chinese cabbage, the Be very effective of aphid is better than the control efficiency of people's urine, but the control efficiency of too late tripterygium root and polygonum flaccidum stem.Variable concentrations people urinates the effect of aphid on control Chinese cabbage: 10-12 and CK1 is without significant difference in process, namely people to urinate the effect of preventing and treating aphid on Chinese cabbage not remarkable.
aphid lethality (unit: %) during table 1. one pack system test process 24h on Chinese cabbage, cucumber
Process 24h Process 24h
Process 1 31.68±3.64b Process 13 31.85±3.25b
Process 2 33.64±1.91b Process 14 34.08±2.25b
Process 3 41.09±2.16a Process 15 42.53±2.04a
Process 4 13.01±0.53d Process 16 13.72±3.25c
Process 5 14.19±3.49d Process 17 14.81±1.97c
Process 6 18.34±1.02c Process 18 15.17±2.61c
Process 7 4.89±2.26ef Process 19 4.21±0.90de
Process 8 5.15±1.68e Process 20 5.49±1.28de
Process 9 6.02±0.78e Process 21 7.15±0.69d
Process 10 3.08±1.38f Process 22 3.17±1.61e
Process 11 4.26±1.10ef Process 23 3.54±0.91e
Process 12 4.51±0.57ef Process 24 4.98±1.28de
CK1 3.03±0.33f CK2 2.15±0.38e
Note: lowercase is 0.05 level, the different letter representation significant difference of same row, lower same.
On variable concentrations tripterygium root control cucumber, the effect of aphid is: the aphid lethality of process 15 is significantly higher than process 13-14, illustrates that the Be very effective of aphid on higher concentration tripterygium root control cucumber is better than the tripterygium root of low concentration.Process 13-15 is significantly higher than again other process and CK2, and namely on tripterygium root control cucumber, the Be very effective of aphid is better than the control efficiency of polygonum flaccidum stem, Oil-tea-cake, people's urine.
On variable concentrations polygonum flaccidum stem control cucumber, the effect of aphid is: the aphid lethality of process 16-18 is without significant difference, but process 16-18 is significantly higher than process 19-24 and CK2, namely on polygonum flaccidum stem control Chinese cabbage, the Be very effective of aphid is better than the control efficiency of Oil-tea-cake, people's urine, but the control efficiency of too late tripterygium root.On variable concentrations Oil-tea-cake control cucumber, the effect of aphid is: the aphid lethality of process 19-21 is without significant difference, but process 21 is significantly higher than process 22-23 and CK2, on the Oil-tea-cake control Chinese cabbage of i.e. high concentration, the Be very effective of aphid is better than the control efficiency of people's urine, but not as good as the control efficiency of tripterygium root and polygonum flaccidum stem.Variable concentrations people urinates the effect of aphid on control cucumber: 22-24 and CK2 is without significant difference in process, namely people to urinate the effect of preventing and treating aphid on cucumber not remarkable.
Result of the test shows (table 2), and the aphid lethality of process 27 is significantly higher than process 25-26, illustrates that the Be very effective of aphid on higher concentration tripterygium root control radish is better than the tripterygium root of low concentration.Process 25-27 is significantly higher than again other process and CK3, and namely on tripterygium root control radish, the Be very effective of aphid is better than the control efficiency of polygonum flaccidum stem, Oil-tea-cake, people's urine.
On variable concentrations polygonum flaccidum stem control radish, the effect of aphid is: the process 30 of higher concentration and the process 28-30 aphid lethality of low concentration are without significant difference.But the aphid lethality of process 28-30 is significantly higher than process 31-36 and CK3, namely on polygonum flaccidum stem control radish, the Be very effective of aphid is better than the control efficiency of Oil-tea-cake, people's urine, but the control efficiency of too late tripterygium root.On variable concentrations Oil-tea-cake control radish, the effect of aphid is: the process 33 of higher concentration and the process 31-32 aphid lethality of low concentration are without significant difference, but process 31-33 is significantly higher than CK3, namely Oil-tea-cake prevents and treats the Be very effective of aphid on radish, but the control efficiency of too late tripterygium root and polygonum flaccidum stem.Variable concentrations people urinates the effect of aphid on control radish: 34-36 and CK3 is without significant difference in process, namely people to urinate the effect of preventing and treating aphid on radish not remarkable.
aphid lethality (unit: %) during table 2. one pack system test process 24h on radish, fresh kidney beans
Process 24h Process 24h
Process 25 31.93±2.18b Process 37 24.27±0.80c
Process 26 32.58±0.81b Process 38 30.15±1.41b
Process 27 10.80±5.33a Process 39 51.87±1.67a
Process 28 23.72±2.15c Process 40 20.43±4.47c
Process 29 23.84±2.96c Process 41 21.39±2.15c
Process 30 25.27±1.41c Process 42 24.80±0.62c
Process 31 5.61±0.91de Process 43 4.07±1.27e
Process 32 8.10±1.78d Process 44 7.64±0.78de
Process 33 7.85±2.29d Process 45 9.81±1.90d
Process 34 3.17±0.71e Process 46 2.90±1.02e
Process 35 3.61±1.01e Process 47 3.17±1.27e
Process 36 4.5±1.28de Process 48 3.64±0.58e
CK3 1.10±0.57e CK4 3.25±0.39e
On variable concentrations tripterygium root control fresh kidney beans, the effect of aphid is: the aphid lethality of process 39 is significantly higher than process 37-38, illustrates that the Be very effective of aphid on higher concentration tripterygium root control fresh kidney beans is better than the tripterygium root of low concentration.Process 37-39 is significantly higher than again other process and CK4, and namely on tripterygium root control fresh kidney beans, the Be very effective of aphid is better than the control efficiency of polygonum flaccidum stem, Oil-tea-cake, people's urine.
On variable concentrations polygonum flaccidum stem control fresh kidney beans, the effect of aphid is: the aphid lethality of process 40-42 is without significant difference, but process 40-42 is significantly higher than process 43-48 and CK4, namely on polygonum flaccidum stem control fresh kidney beans, the Be very effective of aphid is better than the control efficiency of Oil-tea-cake, people's urine, but the control efficiency of too late tripterygium root.On variable concentrations Oil-tea-cake control fresh kidney beans, the effect of aphid is: the aphid lethality of process 45 is significantly higher than process 43, illustrate that the Be very effective of aphid on higher concentration Oil-tea-cake control fresh kidney beans is better than the Oil-tea-cake of low concentration, but the control efficiency of too late tripterygium root and polygonum flaccidum stem.Variable concentrations people urinates the effect of aphid on control fresh kidney beans: 46-48 and CK4 is without significant difference in process, namely people to urinate the effect of preventing and treating aphid on fresh kidney beans not remarkable.
Shown by above one pack system test, its Be very effective of aphid on higher concentration tripterygium root control Chinese cabbage, cucumber, radish, fresh kidney beans.Along with the reduction of concentration, control efficiency significantly declines.Secondly its control efficiency is followed successively by polygonum flaccidum stem, Oil-tea-cake.Independent employment urine, its control efficiency is not remarkable.Along with the reduction of concentration, control efficiency significantly declines.One pack system test also shows, each component is used alone, and its control efficiency is all lower than 50%.
compatibility mechanism
Compatibility mechanism mainly qualitative and quantitative measures the effect test that each ingredients prevents and treats organic vegetable aphid.
materials and methods
2.1.1 test material
Test vegetable species select the most easily break out aphid Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables.Test ingredients is: tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake.
test method
The preparation of test material: material tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size, is soaked in proportion in urine, add a cover after mixing and soak 15-30d.Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, people's urine deposits the pure urine Weight computation (wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent) of 30d by the ight soil that do not adulterate etc.Add a cover after mixing and soak 15d.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.This organic vegetable control of aphids agent is used before insect pest occurs, and prevention is by every 667m 2the 10 times of liquid sprayings of 5 ~ 10kg stoste; Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 10 ~ 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.
The most preferred mass ratio of above Four composition tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is 3.5:1.5:7.5:0.75, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 1-30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15-30d.Add a cover and soak after 15-30d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.This organic vegetable control of aphids agent is used before insect pest occurs, and prevention is by every 667m 2the 10 times of liquid sprayings of 5 ~ 10kg stoste; Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 10 ~ 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate the recovery of plant injury, growth promoting effects.When breaking out aphid, lid being opened, taking out stoste, by every 667m 25,10,10 times of liquid (referring to amount of water) sprayings of 15kg stoste 3 concentration gradients.
Test is carried out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When test effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.Test arranges 36 process, 4 contrasts, each process 3 repetition, each repetition 0.10m 2, random distribution.Using Percent mortality as treatment effect, simultaneously in conjunction with field observation.The proportioning of each ingredients and amount of application are shown in the following description to each process.
Process 1: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 25kg uses; Process 2: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 210kg uses; Process 3: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 215kg uses; Process 4: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 25kg uses; Process 5: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg uses; Process 6: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 215kg uses; Process 7: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 25g uses; Process 8: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 210g uses; Process 9: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 215g uses; CK1: the contrast of Chinese cabbage, not spray pharmacy, water spray.Process 10: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 25kg uses; Process 11: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 210kg uses; Process 12: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 215kg uses; Process 13: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 25kg uses; Process 14: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg uses; Process 15: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 215kg uses; Process 16: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 25g uses; Process 17: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 210g uses; Process 18: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 215g uses; CK2: the contrast of cucumber, not spray pharmacy, water spray.Process 19: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 25kg uses; Process 20: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 210kg uses; Process 21: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 215kg uses; Process 22: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 25kg uses; Process 23: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg uses; Process 24: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 215kg uses; Process 25: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 25g uses; Process 26: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 210g uses; Process 27: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 215g uses; CK3: the contrast of radish, not spray pharmacy, water spray.Process 28: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 25kg uses; Process 29: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 210kg uses; Process 30: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0:1.0:6.0:0.5 is by every 667m 215kg uses; Process 31: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 25kg uses; Process 32: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg uses; Process 33: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 215kg uses; Process 34: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 25g uses; Process 35: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 210g uses; Process 36: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 4.0:2.0:8.0:1.0 is by every 667m 215g uses; CK4: the contrast of fresh kidney beans, not spray pharmacy, water spray.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.From table 3-4, the Be very effective that process 1-9 prevents and treats aphid on Chinese cabbage is better than CK1, the effect of process 4-6 is significantly better than again processing 1-3, with process 7-9 on the whole without significant difference, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than tripterygium root, polygonum flaccidum stem, people urine, Oil-tea-cake mass ratio be that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake be the compatibility of 3.5:1.5:7.5:0.75 with tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake be its control efficiency of compatibility of 4.0:2.0:8.0:1.0 without significant difference, namely tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake is that the compatibility of 4.0:2.0:8.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is the compatibility of 3.5:1.5:7.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
aphid lethality (unit: %) during table 3. ingredients test process 24h on Chinese cabbage, cucumber
Process 24h Process 24h
Process 1 69.25±5.07c Process 10 65.28±3.27d
Process 2 70.61±3.09c Process 11 76.35±3.62cd
Process 3 78.32±0.71bc Process 12 80.14±1.49cd
Process 4 82.36±2.70b Process 13 84.60±2.18c
Process 5 90.13±1.82ab Process 14 92.16±3.30ab
Process 6 95.30±4.16a Process 15 96.34±4.21a
Process 7 84.20±1.80b Process 16 90.27±0.59ab
Process 8 93.07±2.58ab Process 17 96.43±3.10a
Process 9 98.16±3.55a Process 18 98.10±1.91a
CK1 3.03±1.27d CK2 2.15±0.60e
The Be very effective that process 10-18 prevents and treats aphid on cucumber is better than CK2, the effect of process 13-15 is significantly better than again processing 10-12, with process 16-18 on the whole without significant difference, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than tripterygium root, polygonum flaccidum stem, people urine, Oil-tea-cake mass ratio be that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake be the compatibility of 3.5:1.5:7.5:0.75 with tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake be its control efficiency of compatibility of 4.0:2.0:8.0:1.0 without significant difference, namely tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake is that the compatibility of 4.0:2.0:8.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is the compatibility of 3.5:1.5:7.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
The Be very effective that process 19-27 prevents and treats aphid on radish is better than CK3, the effect of process 22-24 is significantly better than again processing 19-21, with process 25-27 on the whole without significant difference, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than tripterygium root, polygonum flaccidum stem, people urine, Oil-tea-cake mass ratio be that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake be the compatibility of 3.5:1.5:7.5:0.75 with tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake be its control efficiency of compatibility of 4.0:2.0:8.0:1.0 without significant difference, namely tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake is that the compatibility of 4.0:2.0:8.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is the compatibility of 3.5:1.5:7.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
aphid lethality (unit: %) during table 4. ingredients test process 24h on radish, fresh kidney beans
Process 24h Process 24h
Process 19 60.22±2.64e Process 28 60.16±0.91c
Process 20 73.15±0.81d Process 29 78.59±3.04bc
Process 21 81.53±2.19c Process 30 83.47±1.22b
Process 22 89.80±1.02b Process 31 85.09±2.60b
Process 23 92.41±0.53ab Process 32 94.20±3.59a
Process 24 96.38±4.27a Process 33 96.76±0.58a
Process 25 88.09±1.93b Process 34 92.33±4.06ab
Process 26 96.84±0.61a Process 35 96.18±0.83a
Process 27 98.46±2.49a Process 36 98.70±1.31a
CK3 1.10±0.53f CK4 3.25±1.24d
The Be very effective that process 28-36 prevents and treats aphid on fresh kidney beans is better than CK4, the effect of process 31-33 is significantly better than again processing 28-30, with process 34-36 on the whole without significant difference, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency that 5-15kg uses significantly be better than tripterygium root, polygonum flaccidum stem, people urine, Oil-tea-cake mass ratio be that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency that 5-15kg uses, namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the compatibility of 3.0:1.0:6.0:0.5 is by every 667m 2it is the lower limit of its compatibility concentration that 5kg uses.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake be the compatibility of 3.5:1.5:7.5:0.75 with tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake be its control efficiency of compatibility of 4.0:2.0:8.0:1.0 without significant difference, namely tripterygium root, polygonum flaccidum stem, people urinate, the mass ratio of Oil-tea-cake is that the compatibility of 4.0:2.0:8.0:1.0 is by every 667m 2it is the upper limit of its compatibility concentration that 15kg uses.Consider from cost and control efficiency 2 aspects, the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is the compatibility of 3.5:1.5:7.5:0.75, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, be good optimization formula ratio and amount of application.
From the above test to Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetable aphids, the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0 are by every 667m 2it is the upper limit and the lower limit of its control efficiency that 5-15kg uses.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 25kg uses, and it is cost-saving, and raw material, usage amount are all moderate, and control efficiency is good again, is good optimization formula ratio and amount of application.From table 1-2 and the comparative analysis showing 3-4, same by every 667m 2the control efficiency of 5-15kg amount of application, the control efficiency of its table 3-4 is significantly better than the control efficiency showing 1-2, and namely the control efficiency of ingredients is significantly better than the control efficiency of single dose; And the control efficiency of ingredients is not equal to the summation of the control efficiency of each single dose of composition ingredients, the control efficiency of ingredients is greater than the summation of the control efficiency of each single dose of composition ingredients, namely the compatibility of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake has synergy, and the control efficiency namely after its compatibility has synergistic effect.
auxiliary agent is tested
Auxiliary agent is tested mainly qualitative and quantitative and is measured auxiliary agent to the impact of the control efficiency of whole ingredients.
materials and methods
3.1.1 test material
Test vegetable species is Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables.Test ingredients is tripterygium root, polygonum flaccidum stem, people's urine, and auxiliary agent is Oil-tea-cake.
test method
Test is carried out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When test effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Each treatment agent is sprayed with sprayer by 10 times of liquid (referring to amount of water).
Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.Test arranges 12 process, 4 contrasts, each process 3 repetition, each repetition 0.10m 2, random distribution.Using Percent mortality as treatment effect, simultaneously in conjunction with field observation.The preparation of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake and brewing method the same (same with 2.1.2), the proportioning of each ingredients and amount of application are shown in the following description to each process.
Process 1: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg sprays; Process 2: the mass ratio of the aphid of Chinese cabbage---tripterygium root, polygonum flaccidum stem, people's urine is that 3.5:1.5:7.5 is by every 667m 210kg sprays; Process 3: the aphid of Chinese cabbage---the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 210kg sprays; CK1: the contrast of Chinese cabbage, not spray pharmacy, water spray.Process 4: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg sprays; Process 5: the mass ratio of the aphid of cucumber---tripterygium root, polygonum flaccidum stem, people's urine is that 3.5:1.5:7.5 is by every 667m 210kg sprays; Process 6: the aphid of cucumber---the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 210kg sprays; CK2: the contrast of cucumber, not spray pharmacy, water spray.Process 7: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg sprays; Process 8: the mass ratio of the aphid of radish---tripterygium root, polygonum flaccidum stem, people's urine is that 3.5:1.5:7.5 is by every 667m 210kg sprays; Process 9: the aphid of radish---the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 210kg sprays; CK3: the contrast of radish, not spray pharmacy, water spray.Process 10: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that 3.5:1.5:7.5:0.75 is by every 667m 210kg sprays; Process 11: the mass ratio of the aphid of fresh kidney beans---tripterygium root, polygonum flaccidum stem, people's urine is that 3.5:1.5:7.5 is by every 667m 210kg sprays; Process 12: the aphid of fresh kidney beans---the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 210kg sprays; CK4: the contrast of fresh kidney beans, not spray pharmacy, water spray.
statistical analysis
SAS(6.12 is adopted to test data) carry out statistical analysis.As known from Table 5, on process 1 control Chinese cabbage, the Be very effective of aphid is better than CK1 and process 2-3, and the effect of process 2 is significantly better than process 3 again, and namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the ingredients of 3.5:1.5:7.5:0.75 compatibility is by every 667m 2the control efficiency that 10kg sprays is best, secondly for the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine is the control efficiency of the ingredients of 3.5:1.5:7.5 compatibility, again for the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 2the control efficiency that 10kg sprays.Test shows, only has the control efficiency of auxiliary agent Oil-tea-cake far away from the control efficiency of ingredients process 1-2; Without the control efficiency of the process 2 of the ingredients of auxiliary agent again far away from the control efficiency of process 1 having auxiliary agent ingredients.Illustrate thus, auxiliary agent Oil-tea-cake is prevented and treated aphid on Chinese cabbage and is had significant synergies in this ingredients.
aphid lethality (unit: %) during table 5. auxiliary agent test process 24h on Chinese cabbage, cucumber
Process 24h Process 24h
Process 1 90.13±3.51a Process 4 92.16±1.15a
Process 2 72.84±8.24b Process 5 67.90±3.56b
Process 3 4.26±1.65c Process 6 3.54±0.49c
CK1 3.03±0.49c CK2 2.15±0.38c
On process 4 control cucumber, the Be very effective of aphid is better than CK2 and process 5-6, and the effect of process 5 is significantly better than process 6 again, and namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the ingredients of 3.5:1.5:7.5:0.75 compatibility is by every 667m 2the control efficiency that 10kg sprays is best, secondly for the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine is the control efficiency of the ingredients of 3.5:1.5:7.5 compatibility, again for the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 2the control efficiency that 10kg sprays.Test shows, only has the control efficiency of auxiliary agent Oil-tea-cake far away from the control efficiency of ingredients process 4-5; Without the control efficiency of the process 5 of the ingredients of auxiliary agent again far away from the control efficiency of process 4 having auxiliary agent ingredients.Illustrate thus, auxiliary agent Oil-tea-cake is prevented and treated aphid on cucumber and is had significant synergies in this ingredients.
As known from Table 6, on process 7 control radish, the Be very effective of aphid is better than CK3 and process 8-9, and the effect of process 8 is significantly better than process 9 again, and namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the ingredients of 3.5:1.5:7.5:0.75 compatibility is by every 667m 2the control efficiency that 10kg sprays is best, secondly for the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine is the control efficiency of the ingredients of 3.5:1.5:7.5 compatibility, again for the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 2the control efficiency that 10kg sprays.Test shows, only has the control efficiency of auxiliary agent Oil-tea-cake far away from the control efficiency of ingredients process 7-8; Without the control efficiency of the process 8 of the ingredients of auxiliary agent again far away from the control efficiency of process 7 having auxiliary agent ingredients.Illustrate thus, auxiliary agent Oil-tea-cake is prevented and treated aphid on radish and is had significant synergies in this ingredients.
aphid lethality (unit: %) during table 6. auxiliary agent test process 24h on radish, fresh kidney beans
Process 24h Process 24h
Process 7 92.41±1.91a Process 10 94.20±4.83a
Process 8 75.80±3.80b Process 11 80.14±0.91b
Process 9 3.61±1.07c Process 12 3.17±0.68c
CK3 1.10±0.61c CK4 3.25±1.35c
On process 10 control fresh kidney beans, the Be very effective of aphid is better than CK4 and process 11-12, and the effect of process 11 is significantly better than process 12 again, and namely the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is that the ingredients of 3.5:1.5:7.5:0.75 compatibility is by every 667m 2the control efficiency that 10kg sprays is best, secondly for the mass ratio of tripterygium root, polygonum flaccidum stem, people's urine is the control efficiency of the ingredients of 3.5:1.5:7.5 compatibility, again for the mass ratio of people's urine, Oil-tea-cake is that 7.5:0.75 is by every 667m 2the control efficiency that 10kg sprays.Test shows, only has the control efficiency of auxiliary agent Oil-tea-cake far away from the control efficiency of ingredients process 10-11; Without the control efficiency of the process 11 of the ingredients of auxiliary agent again far away from the control efficiency of process 10 having auxiliary agent ingredients.Illustrate thus, auxiliary agent Oil-tea-cake is prevented and treated aphid on fresh kidney beans and is had significant synergies in this ingredients.
More than test shows (table 5-6), and the difference on effect of adding assistant and not adding assistant is remarkable, and the Be very effective of adding assistant is better than the effect of not adding assistant, and its Be very effective of adding assistant does not reduce.People urinates main as brewed medium, the recovery after simultaneously utilizing its fertilizer efficiency effectively can promote insect pest, is equivalent to carry out a foliar application after the process of spray medicine simultaneously.Test also shows (table 1-6), organic vegetable control of aphids tripterygium root, polygonum flaccidum stem, and compatibility whether effect is all significantly higher than CK; Effect and the CK of used additives do not have significant difference; The mass ratio of auxiliary agent Oil-tea-cake, tripterygium root, polygonum flaccidum stem, people's urine is that the ingredients of 0.75:3.5:1.5:7.5 compatibility is by every 667m 2the control efficiency that 10kg sprays is best, and its compatibility is best compatibility (table 1-6).Test shows, after using 15d, its main functional component wilfordine, general flavone, Tea Saponin etc. do not detect, and period does not descend moderate rain or heavy rain; After using, period following moderate rain or heavy rain more than 1 time, after using 10d, its main functional component wilfordine, general flavone, Tea Saponin etc. do not detect.Thunder godvine ( tripterygium wilfordiihook. f.), Celastraceae, tripterygium plant.Another name is herba fibraureae recisae, yellow cured rattan etc.Liana shrub, main product is in China, Korea, Japan, and China is mainly distributed in various places and southwest on the south the Yangtze river basin, main product in Fujian, Zhejiang, Anhui, the ground such as Henan.In being born in the shade many wet hillside, mountain valley, small stream limit shrub.The hillside that happiness is comparatively shady and cool, the sandy soil deep with slant acidity, soil layer or the most suitable growth of yellow soil.Bitter, pungent, cool in nature, poison greatly.Thunder God Calamus liana shrub, high 1-3 rice, sprig brownish red, has the thin rib of 4-6 root, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ellipse of falling ovum, rectangular ellipse or avette, long 4-7.5 centimetre, wide 3-4 centimetre, the anxious point of tip or short gradually point, the wealthy wedge shape of base portion or circle, there is serration at edge, lateral vein 4-7 couple, slightly goes up curved after reaching leaf margin; The long 5-8 millimeter of petiole, close by rust hair.Circular cone cyme is narrower, long 5-7 centimetre, and wide 3-4 centimetre has 3-5 branch usually.The oval shape of samara, long 1-1.5 centimetre, diameter 1-1.2 centimetre, seed buttress shaft shape, reaches 10 millimeters.Thunder godvine bitter, pungent, cool in nature, poison greatly, has clearing heat and detoxicating, dispelling wind and removing obstruction in the meridians, relaxes the muscles and stimulate the blood circulation, swelling and pain relieving, the effect such as desinsection hemostasis, returns liver, kidney channel, effect is dispelled rheumatism, removes obstruction in channels to relieve pain, swelling and pain relieving, removing toxic substances desinsection.The traditional Chinese medical science is used for the treatment of lepra reaction, rheumatoid arthritis, pulmonary tuberculosis and other chronic lung diseases etc.Thunder godvine has the effects such as antitumor, anti-inflammatory, but it is very toxic, so thunder godvine is restricted in application medically always.Tripterygium root mainly contains wilfordine (wilfordine), celastrus orbiculatus benzyl acid amides (celagbenzine), Triptolide (wilforlide) etc.Polygonum flaccidum ( polygonum flaccidummeissn.) be polygonaceae Polygonum, another name: class burnt grass, flaccid knotweed herb etc., annual herb plant, high 60 ~ 90cm, complete stool scatters gland point and young pilose antler.Stem is upright, or throw oneself on the ground in bottom, usual aubergine, and joint expands, and leaf alternate, has short handle.The wide lanceolar of blade, tip is gradually sharp, base portion wedge shape, two sides by coarse wool, above bottle green, have splay blackspot, ocrea film quality, edge growth seta.Spike is born in Zhi Ding, and bennet is elongated, long 6 ~ 12cm, sagging, flower thinning; Perianth 5 drastic crack, white, scatter green gland point, top takes on a red color; Stamen 7 ~ 8; Ovary 1 Room, style 3 pieces.Achene 3 rib, outsourcing persitent perianth.Summer at florescence.At the florescence in summer, aciculiform blade is, has splayed blackspot, spends as spike sequence is born in Zhi Ding.Whole nation most area all has distribution, is born in nearly waterside, dark and damp place.Polygonum flaccidum has expelling wind and removing dampness, blood stasis removing analgesic, removing toxicity for detumescence, effect of killing parasites to relieve itshing, and the traditional Chinese medical science is used for the treatment of dysentery, gastroenteritis, diarrhoea, rheumatic arthritis, treating swelling and pain by traumatic injury, functional uterine bleeding; Venomous snake bite is controlled in external application, skin eczema etc.Polygonum flaccidum herb is containing flavones monomer rutin (Rutin), Hyperoside (Hyperin), quercetin (Quercetin), Isorhamnetin (Isorhamnetin) and kaempferol (Kaempferol) and volatile oil n-decane, a small amount of nopinene, citrene and n-undecane etc., poisonous.Oil tea ( camellia oleiferaabel.) Theaceae, Camellia is belonged to, another name: tea subtree, tea oil tree etc.Evergreen dungarunga.Because of its seed can extract oil (tea oil) edible, therefore named.Shrub or middle arbor; Spray has coarse wool.Leaf keratin, oval, Long Circle or obovate, tip is sharp and have blunt nosed, sometimes gradually sharp or blunt, base portion wedge shape, long 5-7 centimetre, wide 2-4 centimetre, sometimes longer, above bottle green, shinny, middle arteries and veins has coarse wool or pubescence, below light green color, become mildewed without hair or middle arteries and veins, lateral vein can be shown in above, and below not clearly, there is serration at edge, sometimes have a cognate, the long 4-8 millimeter of petiole, has coarse wool.Tea oil tree is one of large woody oleiferous plants in the world four, and the high mountain of its growth in southern china subtropical zone and hilly country are the senior oil plants of the distinctive a kind of pure natural of China.China extensively cultivates from the Yangtze river basin to various places, south China.Mainly concentrate on Zhejiang, Jiangxi, Henan, Hunan, Guang Xiwu province.Tea oil look clear taste is fragrant, and nutritious, storage tolerance is high-quality edible oil; Also can be used as lubricating oil, antirust oil for industry.Tea cake is agricultural chemicals, is again fertilizer, can improve farmland water-holding capacity and control rice pest.Containing Tea Saponin, tea seed polysaccharide, tea seed albumen etc. in leached tea oil slag, they are all the raw materials of chemical industry, light industry, food, feed industry product etc.Urine: urine about 95% is water, all the other are solid portion.In solids, inorganic substances have the inorganic salts such as sodium, potassium, chlorine, calcium, phosphorus, magnesium and sulphate, phosphate, ammonium salt; Organic substance mainly contains urea, uric acid, hippuric acid, creatine and creatinine, amino acid and ammonia, urokinase, epidermal growth factor, somatotropin, erythropoietin, gonadotropin, callicrein and antitumor peptide matters etc.So tripterygium root, polygonum flaccidum stem, Oil-tea-cake are immersed in urine, inorganic salts, the organic principle of urine can effectively be dissolved in inorganic, the organic principle of tripterygium root, polygonum flaccidum stem, Oil-tea-cake in urine, reach naturally extracted object.
Test shows, the main functional component wilfordine etc. of tripterygium root has good stomach poison function, the functional components such as the general flavone+volatile oil n-decane of polygonum flaccidum stem not only have good action of contace poison, can also promote that the functional components such as wilfordine are in the speed of vegetable aphid toxicity in vivo effect, the two has well collaborative, synergistic effect.The main functional component of tripterygium root, polygonum flaccidum stem have vegetable aphid good to tag, interior suction, the toxic action such as stomach toxicity.The functional components such as the Tea Saponin of Oil-tea-cake not only can effectively dissolve the functional component such as wilfordine, general flavone, significantly improve the toxic action to the tagging of vegetable aphid, interior suction, stomach toxicity etc., Tea Saponins etc. can also form soap-film, the pore of blocking aphid, enhancing the toxic action of tripterygium root, polygonum flaccidum stem, is a kind of well auxiliary agent.
Embodiment
Following embodiment and embodiment are intended to further illustrate the present invention, instead of limitation of the present invention.
The preparation of control agent stoste: tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Then, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size, is soaked in proportion in urine, add a cover after mixing and soak 15d.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0, (wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent).Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15d.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 5 ~ 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.
The most preferred mass ratio of above Four composition tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is 3.5:1.5:7.5:0.75, when breaking out aphid, lid being opened, taking out stoste, by every 667m 210 times of liquid (the referring to amount of water) sprayings of 5 ~ 15kg stoste.Embodiment is implemented in proving ground, Yiyang, selects the plot often sending out aphid insect pest former years to carry out.Prevent and treat when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.
embodiment 1:
The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is that 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency of 5kg control agent stoste 10 times of liquid sprayings:
The preparation of control agent stoste: tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size, is soaked in proportion in urine, add a cover after mixing and soak 15d.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.0:1.0:6.0:0.5, (wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent).Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15d.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 5kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.Carry out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.During process 24h, the percentage of aphid lethality is:
Chinese cabbage is 69.25%, CK is 3.03%, and aphid lethality is higher than CK 22.85 times; Cucumber is 65.28%, CK is 2.15%, and aphid lethality is higher than CK 30.36 times; Radish is 60.22%, CK is 1.10%, and aphid lethality is higher than CK 54.75 times; Fresh kidney beans are 60.16%, CK is 2.15%, and aphid lethality is higher than CK 27.98 times.
Result of implementation shows, organic vegetable control of aphids agent tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake compatibility mass ratio are that 3.0:1.0:6.0:0.5 is by every 667m 2the control efficiency of 5kg control agent stoste 10 times of liquid sprayings is remarkable.
embodiment 2:
The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is that 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency of 10kg control agent stoste 10 times of liquid sprayings:
The preparation of control agent stoste: tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size, is soaked in proportion in urine, add a cover after mixing and soak 15d.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 3.5:1.5:7.5:0.75, (wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent).Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15d.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 10kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.Carry out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.During process 24h, the percentage of aphid lethality is:
Chinese cabbage is 90.13%, CK is 3.03%, and aphid lethality is higher than CK 29.75 times; Cucumber is 92.16%, CK is 2.15%, and aphid lethality is higher than CK 42.87 times; Radish is 92.41%, CK is 1.10%, and aphid lethality is higher than CK 84.01 times; Fresh kidney beans are 94.20%, CK is 2.15%, and aphid lethality is higher than CK 43.81 times.
Result of implementation shows, organic vegetable control of aphids agent tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake compatibility mass ratio are that 3.5:1.5:7.5:0.75 is by every 667m 2the control efficiency of 10kg control agent stoste 10 times of liquid sprayings is remarkable.
embodiment 3:
The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is that 4.0:2.0:8.0:1.0 is by every 667m 2the control efficiency of 15kg control agent stoste 10 times of liquid sprayings:
The preparation of control agent stoste: tripterygium root, polygonum flaccidum stem, Oil-tea-cake, people's urine all should be selected from free of contamination areas of environmental condition such as soil, water, air, tripterygium root, polygonum flaccidum stem, Oil-tea-cake all use fresh and alive material, and people urinates the pure urine that the ight soil etc. that do not adulterate deposits 1-30d.Oil-tea-cake is the dry cake of the moisture absorption of not going mouldy of 1-30d.First the earth on tripterygium root, polygonum flaccidum stem is removed during preparation, do not need washing.Thereafter, with hammer, tripterygium root, polygonum flaccidum stem are smash damage and open and remove, be cut into the segment that about 10cm grows.With hammer, Oil-tea-cake is pounded the fritter of about 5 × 5cm size, is soaked in proportion in urine, add a cover after mixing and soak 15d.The mass ratio of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake Four composition is 4.0:2.0:8.0:1.0, (wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent).Tripterygium root, polygonum flaccidum stem are basis by fresh, and Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, and people's urine deposits the pure urine Weight computation of 30d by the ight soil etc. that do not adulterate.Add a cover after mixing and soak 15d.Add a cover and soak after 15d, 4 layers of filtered through gauze 2 ~ 3 times, collects filtrate, puts into container, seal immediately, be this organic vegetable control of aphids agent stoste, preserve 1-6 month.Use, by every 667m after insect pest occurs 2the 10 times of liquid sprayings of 15kg stoste, comparatively fast can kill aphid, stop insect pest to spread, can also supplement the nutrition such as nitrogen, accelerate to recover.Carry out in proving ground, Yiyang.The plot often sending out aphid insect pest former years is selected to carry out.Control efficiency test is carried out when Chinese cabbage, cucumber, radish and fresh kidney beans aphid are broken out; Contrast is located at same plot.When effect is mainly with the outburst of same vegetable aphid, after spraying medicament, count and statistical analysis with the percentage of aphid lethality, contrast (CK) water spray.Chinese cabbage, cucumber, radish and fresh kidney beans four kinds of vegetables when aphid is broken out, select in the morning or at dusk time carry out the process of spray medicine.Do not sprinkle water in spray application 24h or apply fertilizer, carry out during 24h observing and count.Conventional fertilizer water management after 24h.During process 24h, the percentage of aphid lethality is:
Chinese cabbage is 98.16%, CK is 3.03%, and aphid lethality is higher than CK 32.40 times; Cucumber is 98.10%, CK is 2.15%, and aphid lethality is higher than CK 45.63 times; Radish is 98.46%, CK is 1.10%, and aphid lethality is higher than CK 89.51 times; Fresh kidney beans are 98.70%, CK is 2.15%, and aphid lethality is higher than CK 45.91 times.
Result of implementation shows, organic vegetable control of aphids control agent tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake compatibility mass ratio are that 4.0:2.0:8.0:1.0 is by every 667m 2the control efficiency of 15kg control agent stoste 10 times of liquid sprayings is remarkable.

Claims (7)

1. an organic vegetable control of aphids agent, is characterized in that, described organic vegetable control of aphids agent by tripterygium root, polygonum flaccidum stem, people urinates and auxiliary agent Oil-tea-cake compatibility is brewed forms.
2. a kind of organic vegetable control of aphids agent according to claim 1, is characterized in that, tripterygium root, polygonum flaccidum stem, people's urine and the compatibility mass ratio of auxiliary agent Oil-tea-cake are 3.0 ~ 4.0:1.0 ~ 2.0:6.0 ~ 8.0:0.5 ~ 1.0.
3. a kind of organic vegetable control of aphids agent according to claim 1 and 2, is characterized in that, the preferred mass of tripterygium root, polygonum flaccidum stem, people's urine, Oil-tea-cake is than being 3.5:1.5:7.5:0.75.
4. the organic vegetable control of aphids agent according to claims 1 to 3, it is characterized in that, tripterygium root, polygonum flaccidum stem are basis by fresh, Oil-tea-cake is by the dry cake Weight computation of the moisture absorption of not going mouldy of 1-30d, people's urine deposits the pure urine Weight computation of 1 ~ 30d by the ight soil etc. that do not adulterate, wherein people's urine is brewed medium, and Oil-tea-cake is auxiliary agent.
5. according to the organic vegetable control of aphids agent described in claim 2 to 4, it is characterized in that, use, by every 667m after insect pest occurs 25 ~ 15kg stoste, 10 times of liquid are sprayed.
6. using method according to claim 5, is characterized in that, uses, by every 667m after vegetable aphid occurs 2the 10 times of liquid sprayings of 10kg stoste are more excellent and the amount of application of economy.
7. according to the using method described in claim 5 to 6, it is characterized in that, when vegetable aphid breaks out, spray when selecting in the morning or at dusk, use in rear 24h and do not sprinkle water or apply fertilizer, conventional fertilizer water management after 24h, rain if used within rear 24h, then needed after rain again to process.
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CN105660718B (en) * 2016-01-19 2018-04-06 长沙理工大学 A kind of organic lotus rhizome vegetable insecticide and its application method
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CN105613603B (en) * 2016-02-26 2018-04-06 长沙理工大学 A kind of organic peach vegetalitas black peach aphid insecticide and its application method
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