CN105613603A - Organic peach vegetalitas green peach aphid insecticide and using method thereof - Google Patents

Organic peach vegetalitas green peach aphid insecticide and using method thereof Download PDF

Info

Publication number
CN105613603A
CN105613603A CN201610104495.1A CN201610104495A CN105613603A CN 105613603 A CN105613603 A CN 105613603A CN 201610104495 A CN201610104495 A CN 201610104495A CN 105613603 A CN105613603 A CN 105613603A
Authority
CN
China
Prior art keywords
fresh
branch
leaf
pieris
fructus persicae
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610104495.1A
Other languages
Chinese (zh)
Other versions
CN105613603B (en
Inventor
杨洁
张永强
郭兆武
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Changsha University of Science and Technology
Original Assignee
Changsha University of Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Changsha University of Science and Technology filed Critical Changsha University of Science and Technology
Priority to CN201610104495.1A priority Critical patent/CN105613603B/en
Publication of CN105613603A publication Critical patent/CN105613603A/en
Application granted granted Critical
Publication of CN105613603B publication Critical patent/CN105613603B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/16Ericaceae [Heath or Blueberry family], e.g. rhododendron, arbutus, pieris, cranberry or bilberry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an organic peach vegetalitas green peach aphid insecticide and a using method thereof.The vegetalitas insecticide is prepared from fresh oriental arborvitae leaves, fresh pieris japonica branches and fresh thunder god vine herb which are soaked with human urine, wherein the ratio of the mass of weight of the fresh oriental arborvitae leaves to the fresh pieris japonica branches to the fresh thunder god vine herb to the human urine is 1.0-2.0:2.5-3.5:2.0-4.0:5.5-9.5, and is 1.5:3.0:3.0:7.5 preferentially, the four components are calculated according to the ratio of the mass of weight, and the fresh thunder god vine herb is an auxiliary, and the components have the synergistic interaction effect.The organic peach vegetalitas green peach aphid insecticide has the remarkable effect on killing three aphides of organic peaches and is added with water on the basis that the ratio is 1.0:2.0 according to the dosage of 30-50 kg.667 m<-2> to be diluted for spraying.The organic peach vegetalitas green peach aphid insecticide is stable in dosage form, raw material sources are abundant, production use is convenient, operation is easy, the cost is low, no any chemical pollution is caused, and the requirement for the organic peach production is completely met.

Description

A kind of organic Fructus Persicae vegetalitas black peach aphid insecticide and using method thereof
Technical field
The invention belongs to the practical new technique research and development of agricultural and application, the present invention relates to the research and development of a kind of organic Fructus Persicae vegetalitas black peach aphid insecticide and production application method.
Background technology
Fructus Persicae (AmygdaluspersicaL.) there are mutation and the kinds such as Prunus persicanucipersica Schneider, peento, flowering peach, Prunus persica f. densa, ornamental peaches, belong to defoliation small arbor, high 3-8m; Tree crown is broad and open and flat; Bark chocolate, coarse in flakey time old; Sprig is elongated, without hair, glossy, and green, area without shade is transformed into redness, has a large amount of little hole skin; Hibernaculum cone, top is blunt, outer by pubescence, and normal 2-3 clusters, and centre is leaf bud, and both sides are bud. The oval lanceolar of blade, elliptic-lanceolate or the shape of falling ovum lanceolar, long 7-15cm, wide 2-3.5cm, tip is tapering, base portion width wedge shape, above without hair, minority pubescence is had below or without hair, limb tool serration or rough sawn tooth, increment tool body of gland or Non-gland body between arteries and veins axil; Petiole is sturdy, long 1-2cm, often 1 to several pieces bodies of gland, sometimes Non-gland body of tool. Flower Dan Sheng, open prior to leaf, diameter 2.5-3.5cm; Bennet is extremely short or several without stalk; Calyx tube is bell, by pubescence, rare several without hair, green and have punctation; Sepal is avette, and to Long Circle, nose-circle is blunt, outer by pubescence; The oval shape of petal is oval to wide obovate, pink, is seldom white; Stamen is about 20-30, flower pesticide blush; Style is several isometric with stamen or slightly shorter; Ovary is by pubescence. Fruit shapes and size all have variation, ellipse avette, wide or oblateness, diameter 5-7cm, and length is several equal with width, color and luster change is extremely orange-yellow by pale green white, mask blush on the sunny side of being everlasting, and outside is close by pubescence, rare without hair, substantially, carpopodium is short and gos deep into fruit low-lying area for abdomen seam; Pulp white, light green white, yellow, orange-yellow or red, succulence is savory, sweet or sour-sweet; Core is big, freestone or clingstone, oval or subcircular, and both sides are flat, and top is tapering, and surface has longitudinal and transverse rill and hole; Kernel bitter in the mouth, rare taste is sweet. The month at florescence 3-4, the fruit maturation phase is different because of kind, is generally the 8-9 month. Fructus Persicae originates in China, and Chinese each provinces and regions are extensively cultivated, and also all plant all over the world. Main Economic cultivation area in North China, East China each province, there are Haidian District Beijing, Pinggu county, Jixian Tianjin in the area comparatively concentrated, Mengyin, Shandong, Feicheng, Yidu, Qingdao, Shangshui, Henan, Kaifeng, Funing, Hebei province, Zunhua, Shen Xian, Linzhang, Baoji, Xi'an, Tianshui city, Chengdu, Sichuan, Dalian, Fenghua, Zhejiang, Shanghai Nanhui, the ground such as jiangsu wuxi, Xuzhou.
Fructus Persicae is the raw economy of drought and the ornamental plant of a kind of high-yield and high-efficiency, and various places have cultivates widely, and along with planting site industry restructuring, its cultivated area has the gesture of continuous expansion. In recent years, Fructus Persicae pest and disease damage was on the rise, it has also become the big obstacle that development Fructus Persicae produces. The Major Pests of Fructus Persicae has black peach aphid, hyalopterus arundinis, Fructus Persicae tumor aphid, tetranychus vinenensis zacher, peach leafminer noth, Fructus Persicae ball heavily fortified point coccid etc.
In recent years, peach tree harm is increased the weight of by black peach aphid day by day, has had a strong impact on growth promoter and the yield and quality of peach tree, has become a primary pest of peach tree. There is generation more than 20 in general 1 year in black peach aphid, survives the winter with ovum, it is also possible to aptery viviparous female, survive the winter Herba Spinaciae or with brassicaceous vegetable in clamp on peach tree. Surviving the winter on peach tree with ovum, early spring in next year, Fructus Persicae bud was sprouted to florescence, and ovum starts hatching, is clustered on tender shoots and sucks juice. Late March, to early and middle ten days in April, breeds harm in virginopara mode. Adult and larva cluster blade back, killed blade is vertical volume from leaf margin to blade back, organizes thickness of growing fat, chlorisis, and drains mucus pollution branch and leaf, it is suppressed that young sprout grows, and causes fallen leaves.
At present the prevention and controls of Fructus Persicae insect pest is mainly had: 1. cultural control. Winter removes dry branches and fallen leaves, strikes off thick bark from old tress, wipes out killed branch top, and concentration is burnt, and reduces insect hibernacle. 2. Biological control. Protect Fructus Persicae tumor aphid natural enemy, such as Chrysopa, ladybug etc., few spray or do not spray broad spectrum insecticide medicament as far as possible. 3. chemical prevention. Early spring sprouts at Fructus Persicae bud, and the hibernating egg hatching Sheng phase is the critical period of preventing and treating black peach aphid, now sprays once " dry branch " with pyrethrin pesticide or nitre subunit 3000 times of liquid of heterocyclic insecticide 10% imidacloprid or 2500-3000 times of liquid of 3% Acetamiprid. Harm phase, available 10% imidacloprid 3000-4000 times, or 48% these 2000 times of liquid sprayings happy. It is dry that hydropenia peach garden, mountain region can adopt Bayer 71628 to be coated with, and is first scraped off gently (not hindering bark) by trunk uneducated person's skin, then by folding for hydroscopic sanitary paper 4-6 layer, makes length and width be respectively equal to girth and trunk diameter, then dips in 2-4 times of liquid of 50% Bayer 71628 full for the toilet paper folded. Wrap up on trunk with plastic sheeting. Because drug concentration is high, in operating process should band rubber gloves, wash one's hands with soap after having worked, with anti-poisoning.
Above prevention and controls is all effective, and some even has remarkable result, such as chemical prevention. But these prevention and controls also have its obvious shortcoming, as: cultural control is time-consuming takes a lot of work, and when current rural area green grass or young crops strong labour power is few, problem is more prominent; The single-minded selectivity of Biological control is strong, and preventing and treating is not thorough, can only control the population quantity of insect to a certain extent; Although chemical prevention is respond well, but it is big to pollute residual, does not meet organic production requirement. And this organic Fructus Persicae vegetalitas black peach aphid insecticide overcomes the above shortcoming, this organic Fructus Persicae vegetalitas black peach aphid insecticide raw material is taken from free of contamination natural growing plants, its natural decomposition is fast, residence time is short, easy to use, simple to operate, cost is low, insecticidal effect is notable, without any chemical contamination, complies fully with the requirement producing organic Fructus Persicae.
Summary of the invention
The purpose of the present invention aims to provide a kind of organic Fructus Persicae vegetalitas black peach aphid insecticide, and this insecticide is easy to use, simple to operate, and cost is low, and insecticidal effect is notable. Another object of the present invention aims to provide the preferably using method of above-mentioned organic Fructus Persicae vegetalitas black peach aphid insecticide. It is an object of the invention to be accomplished by:
The insecticide of the present invention is the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis is brewed forms, and wherein the fresh herb of Radix Tripterygii Wilfordii is mainly used as auxiliary agent, acts the effect promoting its insecticidal effect; Urina Hominis is used as brewed solvent. Inventor studies discovery, adds the fresh herb of auxiliary agent Radix Tripterygii Wilfordii and can play good synergistic function. Need it is emphasized that, this fresh leaf of Cacumen Platycladi of organic Fructus Persicae vegetalitas black peach aphid insecticide, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and do not use the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected.
This organic Fructus Persicae vegetalitas black peach aphid method for producing insecticide is: the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis described all should be selected except the free of contamination areas of environmental condition such as soil, water, air except above-mentioned, and soil, water body and fertilizer etc. also want heavy metal free to pollute. First fresh for Cacumen Platycladi leaf, the fresh branch of pieris, Radix Tripterygii Wilfordii fresh herb mass ratio by weight are put in a certain amount of Urina Hominis and soaked during preparation. The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis the weight mass ratio of four components be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5. The above four fresh leaves of component Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis most preferred weight mass ratio be 1.5:3.0:3.0:7.5. The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis are pressed fresh weight mass ratio and are calculated, and Urina Hominis calculates by the weight mass ratio of 1��30d. The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four components mass ratio by weight mixing, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, plastic bag sealing is packed, and can preserve 2-3. When using this organic Fructus Persicae vegetalitas black peach aphid insecticide to kill 3 kinds of aphids of Fructus Persicae, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position. Parasite killing dosage, is generally watered dilution spraying with filtrate by 1.0:2.0, and blade sprays its back side, is all sprayed onto is advisable with worm strain leaf back, branch bar, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position, 3 kinds of aphids of Fructus Persicae can be killed preferably. This insecticide produces upper easy to use, and simple to operate, cost is low, and insecticidal effect is notable, and the shelf-life is longer, packs with plastic bag sealing, can carry out factorial praluction, forms product. The present invention selects the fresh leaf of Cacumen Platycladi, the fresh branch of pieris and the fresh herb of auxiliary agent Radix Tripterygii Wilfordii, brewed medium Urina Hominis, have employed the compatibility of suitable quality, is configured to commercial botanical pesticide, and it has significant insecticidal effect. This organic Fructus Persicae vegetalitas black peach aphid insecticide is easy to use, and simple to operate, cost is low, and insecticidal effect is notable, this be other physical measure cannoted than. The following is the comparative test of its insecticidal effect.
Organic Fructus Persicae vegetalitas black peach aphid insecticide of the present invention has no the report of like product at present, and the present invention is the result of the test that inventor draws through experimental study for many years and production practices, and is verified in the production of long period, and effect is preferably. And the formula of the present invention also to have technology content higher, dosage form is more stable, abundant raw material source, easy and simple to handle, as developed into commodity, for producing organic Fructus Persicae, low price. Easy to use aborning, insecticidal effect is notable, and use cost is relatively low, is a kind of good botanical pesticide producing organic Fructus Persicae. Organic Fructus Persicae vegetalitas black peach aphid insecticidal formulation can carry out factorial praluction, and it develops not only will commercially increase a kind of novel organic Fructus Persicae vegetalitas black peach aphid insecticide, also by problem offer one the new solution route difficult for producing organic Fructus Persicae deinsectization at present.
The compound method of the present invention is: first the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, does not need washing, is cut into the segment of 3-5cm length after water is dry. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, and plastic bag sealing is packed, and can preserve 2-3.
The using method of the present invention is: when using this organic Fructus Persicae vegetalitas black peach aphid insecticide parasite killing, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position. Parasite killing dosage, is generally watered dilution spraying with filtrate by 1.0:2.0, and blade sprays its back side, is all sprayed onto is advisable with worm strain leaf back, branch bar, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position, 3 kinds of aphids of Fructus Persicae can be killed preferably. It is typically chosen in before at 11 in fine day morning or sprays after at 15 in afternoon and be advisable, to ensure after spraying and not rain in 24h or manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray. Droplet is as far as possible fine, is limited being formed without relatively large water drop on plant, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation. After spray medicine, in 30d, sylvan life can not carry out the mixed battalion of breeding. Testing inspection shows, uses this organic Fructus Persicae vegetalitas black peach aphid insecticide, and the organic Fructus Persicae of commodity thus produced detects, and the main functional component of its botanical pesticide does not all detect. The following is invention and artificially realize the purpose of the present invention, the comparative test of the insecticidal effect constantly done by the raw material and relevant proportioning thereof of testing the organic Fructus Persicae vegetalitas black peach aphid insecticidal formulation selecting and selecting the applicable present invention.
One pack system is tested
The one pack system test mainly each component of the qualitative test single effect test to organic 3 kinds of aphid killing actions of Fructus Persicae.
Materials and methods
1.1.1 test material
Test is selected and organic Fructus Persicae is produced 3 kinds of aphids that harm is more serious: black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid. Test single dose is: the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, four components of Urina Hominis. Wherein Urina Hominis is brewed medium.
Test method
The preparation of test material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the weight mass ratio of the fresh leaf of Cacumen Platycladi and Urina Hominis is 1.5:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. The weight mass ratio of the fresh branch of pieris and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. The weight mass ratio of the fresh herb of Radix Tripterygii Wilfordii and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. Urina Hominis, with the Urina Hominis of 1��30d, the big Plastic Drum of 25��50L is added a cover to deposit and is not sealed during 30d(deposits). By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, and blade sprays its back side, is all sprayed onto is advisable with worm strain leaf back, branch bar, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position. Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period does not also manually spray. Contrast 3000 times of liquid sprayings of chemical pesticide 10% imidacloprid, viewing test effect after 24h, ensure in 24h after spraying and do not rain, period does not also manually spray. Test effect is mainly with its 3 kinds of aphids: the percentage ratio of black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid mortality rate counts and statistical analysis. Spraying time carries out before aphid is broken out.
Orchard is tested: test arranges 5 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random alignment. Each process is numbered process 1-5 and CK1.
The laboratory test of taeniasis branch and leaf: test arranges 5 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 basin (referring to the basin equipped with taeniasis branch and leaf), respectively processes random alignment. Each process is numbered process 6-10 and CK2. Each disposition is as follows:
The weight mass ratio processing the 1 fresh leaf of Cacumen Platycladi and Urina Hominis is 1.5::7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. The weight mass ratio processing the 2 fresh branches of pieris and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. The weight mass ratio processing the 3 fresh herbs of Radix Tripterygii Wilfordii and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 4 Urina Hominis of 1��30d, the big Plastic Drum of 25��50L is added a cover to deposit and is not sealed during 30d(deposits). By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed by this urine-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 5 and use 3000 times of liquid 40kg.667m of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK1) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h. The weight mass ratio processing the 6 fresh leaves of Cacumen Platycladi and Urina Hominis is 1.5::7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. The weight mass ratio processing the 7 fresh branches of pieris and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. The weight mass ratio processing the 8 fresh herbs of Radix Tripterygii Wilfordii and Urina Hominis is 3.0:7.5, adds a cover immersion with the big Plastic Drum of Urina Hominis 25��50L of 1��30d and soaks not sealing during 30d(soaks), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed with this filtrate-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 9 Urina Hominis of 1��30d, the big Plastic Drum of 25��50L is added a cover to deposit and is not sealed during 30d(deposits). By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate. Plastic bag sealing packaging preserves 3 years. 40kg.667m is pressed by this urine-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 10 and use 3000 times of liquid 40kg.667m of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK2) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h.
Statistical analysis
Test data is adopted SAS(6.12) carry out statistical analysis. Result of the test shows (table 1), the black peach aphid mortality rate processing 1-5 is significantly higher than CK1, process 6-8,10 black peach aphid mortality rate be significantly higher than process 9 and CK2, process 5, process 10 black peach aphid mortality rate be significantly higher than other process with CK1, CK2, process 1-3,6-8 black peach aphid mortality rate be also significantly greater than other process with CK1, CK2. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii black peach aphid mortality rate be all significantly higher than Urina Hominis and CK1, CK2, but be substantially less than the process 5 of chemical pesticide 10% imidacloprid, process 10; The Urina Hominis of orchard test processes and is significantly higher than CK1, and the Urina Hominis of the laboratory test of taeniasis branch and leaf processes the black peach aphid mortality rate with CK2 without significant difference.
Table 1. one pack system is testedThe mortality rate (unit: %) of three kinds of aphids
Process Black peach aphid Hyalopterus arundinis Fructus Persicae tumor aphid
Process 1 24.87��3.90e 30.21��0.89d 28.69��0.67d
Process 2 41.21��1.93cd 43.25��3.34b 34.30��6.10c
Process 3 40.63��0.73cd 42.57��4.69b 25.59��1.92e
Process 4 3.97��0.57g 2.30��0.92f 1.46��0.52g
Process 5 93.46��5.29b 95.87��7.96a 90.30��2.97a
CK1 1.65��0.59h 0.66��0.14g 0.93��0.13g
Process 6 20.58��1.91f 27.60��2.56e 22.74��3.40f
Process 7 43.47��3.45c 41.21��2.80bc 39.15��4.37b
Process 8 39.38��2.09d 36.05��1.49c 26.97��0.76de
Process 9 2.80��0.11gh 0.73��0.31g 1.46��0.19g
Process 10 97.88��4.13a 97.02��2.28a 93.07��4.58a
CK2 0.75��0.17h 1.02��0.31fg 1.15��0.23g
Note: lower case is 0.05 level, with string difference letter representation significant difference, lower same.
Process 1-3,5,6-8,10 hyalopterus arundinis mortality rate be significantly higher than other process with CK1, CK2, process 5, process the hyalopterus arundinis mortality rate of 10 and be significantly higher than other and process and CK1, CK2, process hyalopterus arundinis mortality rate and CK1, CK2 of 9 without significant difference (table 1). Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii hyalopterus arundinis mortality rate be all significantly higher than Urina Hominis and CK1, CK2, but be substantially less than the process 5 of chemical pesticide 10% imidacloprid, process 10; The Urina Hominis of the laboratory test of taeniasis branch and leaf processes the hyalopterus arundinis mortality rate with CK1, CK2 without significant difference.
Process 1-3,5,6-8,10 Fructus Persicae tumor aphid mortality rate be significantly higher than other process with CK1, CK2, process 5, process the Fructus Persicae tumor aphid mortality rate of 10 and be significantly higher than other and process and CK1, CK2, process 4, process Fructus Persicae tumor aphid mortality rate and CK1, CK2 of 9 without significant difference (table 1). Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii Fructus Persicae tumor aphid mortality rate be all significantly higher than Urina Hominis and CK1, CK2, but be substantially less than the process 5 of chemical pesticide 10% imidacloprid, process 10; The Urina Hominis that the laboratory test of taeniasis branch and leaf and orchard are tested processes the Fructus Persicae tumor aphid mortality rate with CK1, CK2 without significant difference.
All shown by the laboratory test of the test of above one pack system orchard with taeniasis branch and leaf, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii the mortality rate of three kinds of black peach aphid worms be all significantly higher than Urina Hominis and process and CK1, CK2, but be substantially less than chemical pesticide 10% imidacloprid and process. Urina Hominis processes the mortality rate of CK1, the CK2 with water spray without significant difference.
Compatibility mechanism
Compatibility mechanism is mainly qualitative and quantitative and tests each ingredients to killing the composite effect test endangering 3 kinds of more serious aphids of organic Fructus Persicae.
Materials and methods
2.1.1 test material
Test is selected and organic Fructus Persicae is produced 3 kinds of aphids that harm is more serious: black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid. Test ingredients is the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, four components of Urina Hominis. Wherein the fresh herb of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.
Test method
The preparation of test material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution. Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, and blade sprays its back side, is all sprayed onto is advisable with worm strain leaf back, branch bar, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position. Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period does not also manually spray. Contrast 3000 times of liquid sprayings of chemical pesticide 10% imidacloprid, viewing test effect after 24h, ensure in 24h after spraying and do not rain, period does not also manually spray. Test effect is mainly with its 3 kinds of aphids: the percentage ratio of black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid mortality rate counts and statistical analysis. Spraying time carries out before aphid is broken out.
Orchard is tested: test arranges 4 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random alignment. Each process is numbered process 1-4 and CK1.
The laboratory test of taeniasis branch and leaf: test arranges 4 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 basin (referring to the basin equipped with taeniasis branch and leaf), respectively processes random alignment. Each process is numbered process 5-8 and CK2. Each disposition is as follows:
Process the 1 fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0:2.5:2.0:5.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 2 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 3 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 2.0:3.5:4.0:9.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 4 and use 3000 times of liquid 40kg.667m of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK1) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h. Process the 5 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0:2.5:2.0:5.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 6 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 7 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 2.0:3.5:4.0:9.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 8 and use 3000 times of liquid 40kg.667m of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK2) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h.
Statistical analysis
Test data is adopted SAS(6.12) carry out statistical analysis. Result of the test shows (table 2), each black peach aphid mortality rate processed is significantly higher than CK1, CK2, process 4, the black peach aphid mortality rate of 7-8 is significantly higher than process 1-2,5 and CK1, CK2, the black peach aphid mortality rate processing 2-3 is significantly higher than process 1, process the mortality rate of 2-3 without significant difference, process the mortality rate of 6-8 without significant difference. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5, it is watered dilution spraying by 1.0:2.0 by this compatibility liquid stock solution, orchard after 24h is notable to the killing action of black peach aphid with the effect of the laboratory test of taeniasis branch and leaf, spraying with 3000 times of liquid of chemical pesticide 10% imidacloprid, the test effect after 24h is essentially identical. Wherein, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the orchard test effect of the taeniasis branch and leaf that weight mass ratio is 1.5:3.0:3.0:7.5 be significantly better than the test effect that weight mass ratio is 1.0:2.5:2.0:5.5 of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component, but with the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the effect that weight mass ratio is 2.0:3.5:4.0:9.5 essentially identical. From economic benefit in conjunction with insecticidal effect consider, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the compatibility that weight mass ratio is 1.5:3.0:3.0:7.5 be the preferably ingredients killing black peach aphid.
Table 2. compatibility mechanismThe mortality rate (unit: %) of three kinds of aphids
Process Black peach aphid Hyalopterus arundinis Fructus Persicae tumor aphid
Process 1 84.82��2.53c 91.70��1.67c 83.75��3.05d
Process 2 92.60��1.56b 95.59��3.96b 88.60��2.17bc
Process 3 94.93��3.28ab 97.26��0.60ab 89.57��0.99ab
Process 4 96.04��0.38a 98.51��2.83a 90.73��4.74a
CK1 1.42��0.81d 0.79��0.32d 1.26��0.43e
Process 5 93.60��3.15b 91.52��1.82c 86.40��2.64c
Process 6 95.03��1.46ab 97.64��4.71ab 89.36��1.30ab
Process 7 96.82��4.60a 98.23��2.02a 88.95��0.97b
Process 8 97.46��2.18a 99.29��3.18a 91.18��3.06a
CK2 0.96��0.24d 0.89��0.13d 0.93��0.24e
Each hyalopterus arundinis mortality rate processed is significantly higher than CK1, CK2, process 8, the hyalopterus arundinis mortality rate of 7-8 is significantly higher than process 1-2,5 and CK1, CK2, the hyalopterus arundinis mortality rate processing 2-3 is significantly higher than process 1, process the hyalopterus arundinis mortality rate between 2-3 without significant difference, the hyalopterus arundinis mortality rate processing 6-7 is significantly higher than process 5, processes the hyalopterus arundinis mortality rate between 6-7 without significant difference. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5, it is watered dilution spraying by 1.0:2.0 by this compatibility liquid stock solution, orchard test after 24h is notable to the killing action of hyalopterus arundinis with the effect of the laboratory test of taeniasis branch and leaf, spraying with 3000 times of liquid of chemical pesticide 10% imidacloprid, the test effect after 24h is essentially identical. Wherein, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the orchard that weight mass ratio is 1.5:3.0:3.0:7.5 test and the laboratory test effect of taeniasis branch and leaf be significantly better than the test effect that weight mass ratio is 1.0:2.5:2.0:5.5 of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component, but with the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the effect that weight mass ratio is 2.0:3.5:4.0:9.5 essentially identical. From economic benefit in conjunction with insecticidal effect consider, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the compatibility that weight mass ratio is 1.5:3.0:3.0:7.5 be the preferably ingredients killing hyalopterus arundinis.
Each Fructus Persicae tumor aphid mortality rate processed is significantly higher than CK1, CK2, the Fructus Persicae tumor aphid mortality rate processing 4,8 is significantly higher than process 1-2,5,7 and CK1, CK2, the Fructus Persicae tumor aphid mortality rate processing 2-3 is significantly higher than process 1, process the Fructus Persicae tumor aphid mortality rate between 2-3 without significant difference, the Fructus Persicae tumor aphid mortality rate processing 6-7 is significantly higher than process 5, processes the Fructus Persicae tumor aphid mortality rate between 6-7 without significant difference. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5, it is watered dilution spraying by 1.0:2.0 by this compatibility liquid stock solution, orchard test after 24h is notable to the killing action of Fructus Persicae tumor aphid with the effect of the laboratory test of taeniasis branch and leaf, spraying with 3000 times of liquid of chemical pesticide 10% imidacloprid, the test effect after 24h is essentially identical. Wherein, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the orchard that weight mass ratio is 1.5:3.0:3.0:7.5 test and the laboratory test effect of taeniasis branch and leaf be significantly better than the test effect that weight mass ratio is 1.0:2.5:2.0:5.5 of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component, but with the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the effect that weight mass ratio is 2.0:3.5:4.0:9.5 essentially identical. From economic benefit in conjunction with insecticidal effect consider, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component the compatibility that weight mass ratio is 1.5:3.0:3.0:7.5 be the preferably ingredients killing Fructus Persicae tumor aphid.
More than test is it is also shown that the mortality rate (table 2) of three kinds of black peach aphid worms of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility test is significantly higher than the mortality rate (table 1) of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component single dose test three kinds of aphids of Fructus Persicae. It is indicated above that the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility have good synergistic function.
Dosetest
Dosetest is mainly qualitative and quantitative and tests each using dosage to killing the effect test endangering 3 kinds of more serious aphids of organic Fructus Persicae.
Test material
Test is selected and organic Fructus Persicae is produced 3 kinds of aphids that harm is serious: black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid. Test ingredients is the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, four components of Urina Hominis. Wherein the fresh herb of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.
Test method
The preparation of test material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution. Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, and blade sprays its back side, is all sprayed onto is advisable with worm strain leaf back, branch bar, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position. Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period does not also manually spray. Contrast 3000 times of liquid sprayings of chemical pesticide 10% imidacloprid, viewing test effect after 24h, ensure in 24h after spraying and do not rain, period does not also manually spray. Test effect is mainly with its 3 kinds of aphids: the percentage ratio of black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid mortality rate counts and statistical analysis. Spraying time carries out before aphid is broken out.
Orchard is tested: test arranges 4 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random alignment. Each process is numbered process 1-4 and CK1.
The laboratory test of taeniasis branch and leaf: test arranges 4 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 basin (referring to the basin equipped with taeniasis branch and leaf), respectively processes random alignment. Each process is numbered process 5-8 and CK2. Each disposition is as follows:
Process the 1 fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 30kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 2 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 3 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 50kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 4 and press 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK1) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h. Process the 5 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 30kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 6 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 7 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 50kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 8 and press 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK2) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h.
Statistical analysis
Test data is adopted SAS(6.12) carry out statistical analysis. Result of the test shows (table 3), and the black peach aphid mortality rate processing 1-4,5-8 is all remarkably higher than CK1, CK2. The black peach aphid mortality rate processing 2-4,6-8 is significantly higher than process 1,5 and CK1, CK2, processes between 2-3,6-8 without significant difference; Process 2 and be significantly higher than process 1; Process 6 and be significantly higher than process 5. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 30-50kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, orchard test after 24h is notable to the killing action of black peach aphid with the laboratory test effect of taeniasis branch and leaf, laboratory test is sprayed with 3000 times of liquid of chemical pesticide 10% imidacloprid, and the test effect observed after 24h is essentially identical. Wherein, compatibility liquid stock solution presses 40kg.667m-2The orchard test effect of dosage is significantly better than compatibility liquid stock solution by 30kg.667m-2The test effect of dosage, but press 50kg.667m with compatibility liquid stock solution-2The laboratory test effect of the orchard of dosage and taeniasis branch and leaf is without significant difference. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stock solution presses 40kg.667m-2Dosage be the preferably dosage killing black peach aphid.
Table 3. dosetestThe mortality rate (unit: %) of three kinds of aphids
Process Black peach aphid Hyalopterus arundinis Fructus Persicae tumor aphid
Process 1 85.61��1.42d 89.05��1.20c 81.37��0.68c
Process 2 92.60��0.39bc 95.59��6.17bc 88.60��2.27ab
Process 3 94.51��3.61b 96.29��4.52b 89.86��1.09a
Process 4 96.04��2.18a 98.51��3.14a 90.73��1.42a
CK1 1.42��0.53e 0.79��0.31e 1.26��0.41d
Process 5 90.34��2.94c 81.40��6.14d 83.76��3.97b
Process 6 95.03��1.82ab 97.64��1.21ab 89.36��0.20a
Process 7 97.61��0.79a 98.70��2.11a 90.92��3.89a
Process 8 97.46��1.16a 99.29��3.07a 91.18��0.38a
CK2 0.96��0.93e 0.89��0.24e 0.93��0.15d
The hyalopterus arundinis mortality rate processing 1-4,5-8 is all remarkably higher than CK1, CK2. Process between 2-3 without significant difference, process between 6-8 without significant difference (table 3); Process 2,6 and be substantially better than process 1,5. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 30-50kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the orchard test after 24h and the effect of the laboratory test of taeniasis branch and leaf are notable to the killing action of hyalopterus arundinis, and with by the 3000 times of liquid sprayings of 10% imidacloprid, the test effect observed after 24h is essentially identical. Wherein, compatibility liquid stock solution presses 40kg.667m-2The laboratory test effect of the taeniasis branch and leaf of dosage is significantly better than compatibility liquid stock solution by 30kg.667m-2The laboratory test effect of the taeniasis branch and leaf of dosage, but press 50kg.667m with compatibility liquid stock solution-2The orchard test of dosage is essentially identical with the laboratory test effect of taeniasis branch and leaf. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stock solution presses 40kg.667m-2Dosage be the preferably dosage killing hyalopterus arundinis.
The Fructus Persicae tumor aphid mortality rate processing 1-4,5-8 is all remarkably higher than CK1, CK2. Process without significant difference between 2-4, process between 6-8 without significant difference (table 3); Process 2,6 and be significantly higher than process 1,5. Described above, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 30-50kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the orchard test after 24h and the effect of the laboratory test of taeniasis branch and leaf are notable to the killing action of Fructus Persicae tumor aphid, and with by the 3000 times of liquid sprayings of 10% imidacloprid, the test effect observed after 24h is essentially identical. Wherein, compatibility liquid stock solution presses 40kg.667m-2The orchard test of dosage is significantly better than compatibility liquid stock solution by 30kg.667m with the laboratory test effect of taeniasis branch and leaf-2The test effect of dosage, but press 50kg.667m with compatibility liquid stock solution-2The laboratory test effect of the orchard of dosage and taeniasis branch and leaf is without significant difference. Considering from economic benefit in conjunction with insecticidal effect, compatibility liquid stock solution presses 40kg.667m-2Dosage be the preferably dosage killing Fructus Persicae tumor aphid.
More than test show, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered the effect that the orchard test of dilution spraying and the laboratory test of taeniasis branch and leaf kill 3 kinds of aphids of Fructus Persicae and be significantly better than 30kg.667m-2The effect of dosage spray, but and 50kg.667m-2The effect of dosage spray is essentially identical. Consider from economic benefit in conjunction with insecticidal effect, 40kg.667m-2Dosage spray is preferably dosage.
More than test is it is also shown that the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, the insecticidal effect of Urina Hominis four component compatibility dosetest and the mortality rate (table 2-3) of 3 kinds of aphids of Fructus Persicae are significantly higher than the mortality rate (table 1) of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, the test of Urina Hominis four component single dose. It is indicated above that the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility have good synergistic function.
Auxiliary agent is tested
Whole ingredients is killed the synergistic function endangering 3 kinds of more serious aphids of organic Fructus Persicae by auxiliary agent test mainly qualitative and quantitative test auxiliary agent.
Materials and methods
4.1.1 test material
Test is selected and organic Fructus Persicae is produced 3 kinds of aphids that harm is more serious: black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid. Test ingredients is the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, four components of Urina Hominis. Wherein the fresh herb of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.
Test method
The preparation of test material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution. Parasite killing dosage, uses filtrate 40kg.667m-2It is watered dilution spraying by 1.0:2.0. Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period does not also manually spray. Contrast 3000 times of liquid sprayings of chemical pesticide 10% imidacloprid, viewing test effect after 24h, ensure in 24h after spraying and do not rain, period does not also manually spray. Test effect is mainly with its 3 kinds of aphids: the percentage ratio of black peach aphid, hyalopterus arundinis and Fructus Persicae tumor aphid mortality rate counts and statistical analysis. Spraying time carries out before aphid is broken out.
Orchard is tested: test arranges 3 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random alignment. Each process is numbered process 1-3 and CK1.
The laboratory test of taeniasis branch and leaf: test arranges 3 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 basin (referring to the basin equipped with taeniasis branch and leaf), respectively processes random alignment. Each process is numbered process 4-6 and CK2. Each disposition is as follows:
Process the 1 fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 2 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, Urina Hominis three component weight mass ratio be 1.5:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 3 and press 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK1) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h. Process the 4 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process the 5 fresh leaves of Cacumen Platycladi, the fresh branch of pieris, Urina Hominis three component weight mass ratio be 1.5:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. Viewing test effect after 24h. Process 6 and press 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2Dosage spray, viewing test effect after 24h. Comparison (CK2) is sprayed water 40kg.667m-2Consumption, viewing test effect after 24h.
Statistical analysis
Test data is adopted SAS(6.12) carry out statistical analysis. Result of the test shows (table 4), and the black peach aphid mortality rate processing 1-6 is significantly higher than CK1, CK2, and the black peach aphid mortality rate processing 2,5 is substantially less than process 1,3,4,6, but is significantly higher than CK1, CK2. Described above, without the fresh leaf of compatibility Cacumen Platycladi of auxiliary agent, the fresh branch of pieris, Urina Hominis three component weight mass ratio be 1.5:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the effect of the orchard test after 24h and the laboratory test of taeniasis branch and leaf the mortality rate of black peach aphid is substantially less than the fresh leaf of compatibility Cacumen Platycladi of auxiliary agent, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the effect of the orchard test after 24h and the laboratory test of the taeniasis branch and leaf mortality rate to black peach aphid; It is also significantly lower than with chemical pesticide by 3000 times of liquid every 667m of spraying of 10% imidacloprid2The peach garden mortality rate of the orchard test after the dosage 24h of diluent 40kg with the laboratory test of taeniasis branch and leaf.
Table 4. auxiliary agent is testedThe mortality rate (unit: %) of three kinds of aphids
Process Black peach aphid Hyalopterus arundinis Fructus Persicae tumor aphid
Process 1 92.60��1.46b 95.59��2.49b 88.60��3.92b
Process 2 63.55��4.13c 56.92��1.16d 53.41��0.16c
Process 3 96.04��0.49a 98.51��3.15a 90.73��1.58a
CK1 1.42��0.42e 0.79��0.25e 1.26��0.24e
Process 4 95.03��3.64ab 97.64��3.06ab 89.36��3.10ab
Process 5 51.09��1.08d 68.24��7.04c 50.16��0.35d
Process 6 97.46��7.33a 99.29��0.79a 91.18��4.73a
CK2 0.96��0.32e 0.89��0.33e 0.93��0.37e
The hyalopterus arundinis mortality rate processing 1-6 is significantly higher than CK1, CK2, and the hyalopterus arundinis mortality rate processing 2,5 is substantially less than process 1,3,4,6, but is significantly higher than CK1, CK2(table 4). Described above, without the fresh leaf of compatibility Cacumen Platycladi of auxiliary agent, the fresh branch of pieris, Urina Hominis three component weight mass ratio be 1.5:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the killing action of hyalopterus arundinis is substantially less than the compatibility of auxiliary agent by the effect of the orchard test after 24h and the laboratory test of taeniasis branch and leaf: the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, hyalopterus arundinis is killed mortality rate by the effect of the orchard test after 24h and the laboratory test of taeniasis branch and leaf; It is also significantly lower than with chemical pesticide by 3000 times of liquid every 667m of spraying of 10% imidacloprid2The peach garden laboratory test mortality rate in the orchard after the dosage 24h of diluent 40kg Yu taeniasis branch and leaf.
The Fructus Persicae tumor aphid mortality rate processing 1-6 is significantly higher than CK1, CK2, and the Fructus Persicae tumor aphid mortality rate processing 2,5 is substantially less than process 1,3,4,6, but is significantly higher than CK1, CK2(table 4). Described above, without the compatibility of auxiliary agent: the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, Urina Hominis three component weight mass ratio be 1.5:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, the mortality rate of killing of Fructus Persicae tumor aphid is substantially less than the compatibility of auxiliary agent by the effect of the orchard test after 24h and the laboratory test of taeniasis branch and leaf: the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, press 40kg.667m by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying, Fructus Persicae tumor aphid is killed mortality rate by the effect of the laboratory test of the orchard after 24h and taeniasis branch and leaf; It is also significantly lower than with chemical pesticide by 3000 times of liquid every 667m of spraying of 10% imidacloprid2The peach garden laboratory test mortality rate in the orchard after the dosage 24h of diluent 40kg Yu taeniasis branch and leaf.
More than test shows (table 4), and adding assistant is notable with the difference on effect of not adding assistant, and the effect of adding assistant is significantly better than the effect of not adding assistant, and its effect of adding assistant does not significantly reduce. Illustrate that the fresh herb of auxiliary agent Radix Tripterygii Wilfordii has significant collaborative and potentiation in this compatibility.
Test also shows (table 1-4), and the effect of ingredients is about 1-4 times of each single dose effect. So, the effect after compatibility is not the addition of simple each single dose effect. It is tested to compatibility mechanism from single dose and tests (table 1-4) to auxiliary agent again, it is shown that the compatibility liquid stock solution that weight mass ratio is 1.5:3.0:3.0:7.5 of the fresh leaf of compatibility Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component is by 40kg.667m-2Dosage 1.0:2.0 be watered dilution spraying ingredients, from economic benefit in conjunction with insecticidal effect consider be preferably select. Test shows, with the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility agent spray in the commodity Fructus Persicae of rear 30d and do not detect the compositions such as its main component mountain laurel toxin, wilfordine.
Hold concurrently action of contace poison for stomach poison for this brewed dose, its toxic component Laurel toxin, wilfordine etc. together enter it with the aphid of Fructus Persicae when taking food internal cause that internal metabolism is chaotic and is poisoned to death, or directly directly invaded to cause in aphid body by body surface be poisoned to death with the stronger chemosmotic terpene alcohol that has in ingredients, or directly invade from polypide body surface both sides pore and be transported to each position of polypide through body-internal-circulation in aphid body and finally result in and be poisoned to death.
Cacumen PlatycladiPlatycladusorientalis(L.) Franco �� belong to aiphyllium, bark ficelle or Dark grey, lobe growth silver peels off; Branch is close, and sprig is flat, lines up a plane; Squama shape leaf is alternately to life, and, to flat, there is gland point in front 1, and side 1, to keel-shaped, is placed on the leaf of front; Hermaphroditism, spends and is singly born in brachyplast last year top; Male cone yellow, is made up of decussate microsporophyll, and each microsporophyll has 3 pollen bags, and cone-scale and bract scale heal completely; Female cone has 4 pairs of cone-scales; Cone has fruit scale 4 right, meat before ripe, wooden after maturation, cracking, and thicker, there is the tip of 1 contrary flexure on nearly top, back, and middle part fruit scale is respectively arranged with 1��2 seed; Cone is ripe then, fruit scale lignifying, cracking, and seed does not have wing or has rib ridge; 3��April of florescence, really 10��November of phase. Cacumen Platycladi originates in China, and except Qinghai, Xinjiang, all there is distribution in the whole nation; Cacumen Platycladi is drought-enduring, is often afforestation in sun slope seeds, gas delicate fragrance, bitter and puckery flavor, micro-pungent, containing volatile oil, is mainly fenchone, Camphora, borneol acetate, terpene alcohol in oil; And containing juniperic acid Quercetin, poplar luteoxanthin, kaempferol, hinokiflavone, waxiness etc. Volatile oil contained by it is mainly composed of ��-absinthol, thujene, fenchone etc.; Other is still containing flavones ingredient, such as aromadendrin, Quercetin, myricetin, hinokiflavone etc.
Pieris (Piereispolta) belonging to evergreen shrubs or dungarunga, bark sepia, sprig is carried out, without hair; Hibernaculum obovate, perula 3-8 piece, arrange in imbricate. Leaf is intensive in Zhi Ding; Petiole length 3��8mm, the outside of belly has zanjon; Blade keratin, ellipticity lanceolar, or fall lanceolar, long 3��8cm, wide 1��2cm, tip is short tapering, the narrow wedge shape of base portion, edge more than 2/3 has a thin knuckle-tooth, rare is bordering on full edge, surface bottle green, glossy, back side light green, master pulse is protruding on two sides, lateral vein falls under the surface, and the back side is inconspicuous, and scun is netted. Raceme or panicle top are given birth to or axil is raw, and cluster Yu Zhiding, long 8��14cm, and rachis has pubescence; Sepal triangular shape is avette, is about 3.5mm; Corolla white, urceolus, top 5 is shallow to be split, sliver subcircular; Stamen 10, filigree has long pubescence; Ovary 1, subsphaeroidal, style is elongated, stigma head. Capsule is bordering on oblate spheroid, diameter 3��5mm, loculicidal, and calyx and style harbor, 4��May of florescence, fruit phase 7��JIUYUE. Pieris is distributed widely in the ground such as Zhejiang, Yunnan. Pieris is containing compounds such as diterpene, triterpene and flavone, its branch and leaf are containing compounds such as mountain laurel toxin, the mould glycosides of pieris and black laurel poison glycosides, its root containing 3-oxykojic acid, 3-hydroxyl-2 methylol-4H-pyrans-4-ketone, phloretin, 3,3 ', 4 ', the compound such as 7-tetrahydroxy-5-methoxy flavone, catechol, cupreol.
Radix Tripterygii Wilfordii (TripterygiumwilfordiiHook.f.), Celastraceae, tripterygium plant, liana shrub, main product is in China, Korea, Japan, and China is distributed mainly on various places and southwest on the south the Yangtze river basin, main product in Fujian, Zhejiang, Hubei, Hunan, Anhui, the ground such as Henan. In being born in the shade many wet hillside, mountain valley, small stream limit bushes. The hillside that happiness is comparatively shady and cool, the sand soil deep with slant acidity, soil layer or the most suitable growth of yellow soil. Bitter in the mouth, pungent, cool in nature, poison greatly. Thunder God Calamus liana shrub, high 1��3m, sprig brownish red, there are 4��6 thin ribs, by Mi Mao and fine and closely woven hole skin. Elliptic leaf shape, the ovum ellipse or avette oval, rectangular that falls, long 4��7.5cm, wide 3��4cm, tip is anxious sharp or short tapering, the wealthy wedge shape of base portion or circle, and there are serration, lateral vein 4��7 pairs in edge, slightly goes up curved after reaching leaf margin; Petiole length 5��8mm, close by rust hair. Circular cone cyme is narrower, long 5��7cm, wide 3-4cm, generally has 3��5 branches. The oval shape of Myriopteron extensum (Wight) K. Schum, long 1��1.5cm, diameter 1��1.2cm, seed buttress shaft shape, up to 10mm. Radix Tripterygii Wilfordii is mainly containing wilfordine, Celastrus orbiculatus Thunb. benzyl amide, Triptolide etc.
Detailed description of the invention
Implementation below and embodiment are further intended to illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
The preparation of material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, and plastic bag sealing is packed, and can preserve 2��3 years. Use selection of time spray medicine before the 3 kinds of aphid outbursts of organic Fructus Persicae, it is possible to carry out early prevention. During use, use filtrate 30kg.667m-2It is watered dilution spraying by 1.0:2.0. Selecting before at 11 in fine day morning or after at 15 in afternoon or spray at dusk, not raining after spraying in guarantee 24h, period does not also manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray. Droplet is as far as possible fine, and to be formed without on plant relatively, large water drop is for degree, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation. After spray medicine, in 30d, sylvan life can not carry out the mixed battalion of breeding.
The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 30kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. After 24h, the insecticidal effect in organic peach fruit garden is: black peach aphid mortality rate 85.61%, hyalopterus arundinis mortality rate 89.05%, Fructus Persicae tumor aphid mortality rate 81.37%, presses 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2The insecticidal effect of dosage spray only differ 10.43%, 9.46%, 9.36% respectively, substantially close to the insecticidal effect of chemical pesticide 10% imidacloprid.
Embodiment 2:
The preparation of material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, and plastic bag sealing is packed, and can preserve 2��3 years. Use selection of time spray medicine before the 3 kinds of aphid outbursts of organic Fructus Persicae, it is possible to carry out early prevention. During use, use filtrate 40kg.667m-2It is watered dilution spraying by 1.0:2.0. Selecting before at 11 in fine day morning or after at 15 in afternoon or spray at dusk, not raining after spraying in guarantee 24h, period does not also manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray. Droplet is as far as possible fine, and to be formed without on plant relatively, large water drop is for degree, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation. After spray medicine, in 30d, sylvan life can not carry out the mixed battalion of breeding.
The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 40kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. After 24h, the insecticidal effect in organic peach fruit garden is: black peach aphid mortality rate 92.60%, hyalopterus arundinis mortality rate 95.59%, Fructus Persicae tumor aphid mortality rate 88.60%, presses 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2The insecticidal effect of dosage spray only differ 3.44%, 2.92%, 2.13% respectively, essentially identical with the insecticidal effect of chemical pesticide 10% imidacloprid.
Embodiment 3:
The preparation of material: the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb system of Radix Tripterygii Wilfordii naturally grow and form, and are provided without the mountain area of chemical fertilizer, chemical pesticide. Otherwise, the quality of organic Fructus Persicae will be affected. First the earth on fresh for Cacumen Platycladi leaf, the fresh branch of pieris and the fresh herb of Radix Tripterygii Wilfordii, water are shaked off during preparation, do not need washing, after water is dry, be cut into the segment of 3-5cm length. Thereafter, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, the big Plastic Drum of 25��50L is added a cover during immersion 20��40d(soaks and is not sealed), by 3��4 layers of filtered through gauze after 20��40d, its filtrate is this organic Fructus Persicae vegetalitas black peach aphid insecticide, and plastic bag sealing is packed, and can preserve 2��3 years. Use selection of time spray medicine before the 3 kinds of aphid outbursts of organic Fructus Persicae, it is possible to carry out early prevention. During use, use filtrate 50kg.667m-2It is watered dilution spraying by 1.0:2.0. Selecting before at 11 in fine day morning or after at 15 in afternoon or spray at dusk, not raining after spraying in guarantee 24h, period does not also manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray. Droplet is as far as possible fine, and to be formed without on plant relatively, large water drop is for degree, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation. After spray medicine, in 30d, sylvan life can not carry out the mixed battalion of breeding.
The fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis four component weight mass ratio be 1.5:3.0:3.0:7.5. 50kg.667m is pressed by this compatibility liquid stock solution-2Dosage 1.0:2.0 be watered dilution spraying. After 24h, the insecticidal effect in organic peach fruit garden is: black peach aphid mortality rate 94.51%, hyalopterus arundinis mortality rate 96.29%, Fructus Persicae tumor aphid mortality rate 89.86%, presses 40kg.667m with 3000 times of liquid of chemical pesticide 10% imidacloprid-2The insecticidal effect of dosage spray only differ 1.53%, 2.22%, 0.87% respectively, substantially close to the insecticidal effect of chemical pesticide 10% imidacloprid.

Claims (8)

1. an organic Fructus Persicae vegetalitas black peach aphid insecticide and using method thereof, it is characterised in that described organic Fructus Persicae vegetalitas black peach aphid insecticide by the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, Radix Tripterygii Wilfordii fresh herb compatibility Urina Hominis is brewed forms.
2. one according to claim 1 organic Fructus Persicae vegetalitas black peach aphid insecticide, it is characterized in that, the compatibility weight mass ratio of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of auxiliary agent Radix Tripterygii Wilfordii and brewed medium Urina Hominis is 1.0��2.0:2.5��3.5:2.0��4.0:5.5��9.5.
3. the one organic Fructus Persicae vegetalitas black peach aphid insecticide according to claim 1 and 2, it is characterised in that the preferred weight mass ratio of the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii and Urina Hominis is 1.5:3.0:3.0:7.5.
4. the organic Fructus Persicae vegetalitas black peach aphid insecticide according to claims 1 to 3, it is characterized in that, the fresh leaf of Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii are all by the weight Mass Calculation of fresh material, Urina Hominis is by the weight Mass Calculation of 1��30d, wherein the fresh herb of Radix Tripterygii Wilfordii is auxiliary agent, Urina Hominis is brewed medium, soaks 20��40d, and brewed period can not seal.
5. the organic Fructus Persicae vegetalitas black peach aphid insecticide according to claim 2 to 4, it is characterized in that, the fresh leaf of material Cacumen Platycladi, the fresh branch of pieris, the fresh herb of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from free of contamination areas of environmental condition such as soil, water, air, and soil, water body and fertilizer etc. also want heavy metal free to pollute.
6. the organic Fructus Persicae vegetalitas black peach aphid insecticide according to claim 2 to 4, it is characterized in that, during use, need to ensure that leaf back, branch bar etc. have worm position to be all sprayed onto position, it is typically chosen in before at 11 in fine day morning or sprays after at 15 in afternoon and be advisable, to ensure after spraying and not rain in 24h or manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.
7. using method according to claim 6, is characterized in that, selects to spray medicine before aphid population number does not have great outburst, by 30��50kg.667m-2Dosage 1.0:2.0 be watered dilution spraying, droplet is as far as possible fine, is limited being formed without relatively large water drop on plant, the black peach aphid of organic Fructus Persicae, hyalopterus arundinis and three kinds of aphids of Fructus Persicae tumor aphid can be carried out effective killing action.
8. the using method according to claim 6-7, it is characterized in that, all can use at Fructus Persicae bud sprouting period and aphid damage phase, it is also possible in prevention, after spray medicine, in 30d, sylvan life can not carry out the mixed battalion of breeding, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.
CN201610104495.1A 2016-02-26 2016-02-26 A kind of organic peach vegetalitas black peach aphid insecticide and its application method Expired - Fee Related CN105613603B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610104495.1A CN105613603B (en) 2016-02-26 2016-02-26 A kind of organic peach vegetalitas black peach aphid insecticide and its application method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610104495.1A CN105613603B (en) 2016-02-26 2016-02-26 A kind of organic peach vegetalitas black peach aphid insecticide and its application method

Publications (2)

Publication Number Publication Date
CN105613603A true CN105613603A (en) 2016-06-01
CN105613603B CN105613603B (en) 2018-04-06

Family

ID=56029304

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610104495.1A Expired - Fee Related CN105613603B (en) 2016-02-26 2016-02-26 A kind of organic peach vegetalitas black peach aphid insecticide and its application method

Country Status (1)

Country Link
CN (1) CN105613603B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901031A (en) * 2016-06-07 2016-08-31 长沙理工大学 Botanical plum aphid insecticide for organic plums and use method thereof
CN105901032A (en) * 2016-06-21 2016-08-31 长沙理工大学 Organic loquat botanical phylloxera insecticide and application method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104782674A (en) * 2015-04-29 2015-07-22 长沙理工大学 Aphid removal agent for organic vegetables and using method of aphid removal agent

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104782674A (en) * 2015-04-29 2015-07-22 长沙理工大学 Aphid removal agent for organic vegetables and using method of aphid removal agent

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
姜传义编著,: "《中国杀虫植物志》", 31 December 1999 *
段志坤,: ""沼液及草木灰、人尿在果树上的妙用"", 《山西果树》 *
汤兆成主编,: "《松阳树木彩色图鉴》", 31 October 2013 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901031A (en) * 2016-06-07 2016-08-31 长沙理工大学 Botanical plum aphid insecticide for organic plums and use method thereof
CN105901031B (en) * 2016-06-07 2018-07-10 长沙理工大学 A kind of organic Lee's vegetalitas plum aphid insecticide and its application method
CN105901032A (en) * 2016-06-21 2016-08-31 长沙理工大学 Organic loquat botanical phylloxera insecticide and application method thereof
CN105901032B (en) * 2016-06-21 2018-07-10 长沙理工大学 A kind of organic loquat vegetalitas wood louse insecticide and its application method

Also Published As

Publication number Publication date
CN105613603B (en) 2018-04-06

Similar Documents

Publication Publication Date Title
Hodge et al. The ethnobotany of the island Caribs of Dominica
Raghavan et al. Arecanut: India’s popular masticatory—history, chemistry and utilization
Balls Early uses of California plants
Dharani et al. Common antimalarial trees and shrubs of East Africa
Ur-Rahman et al. Reference guide on high value medicinal and aromatic plants–sustainable management and cultivation practices
Morton The balsam pear—an edible, medicinal and toxic plant
Van Beek et al. Ginkgo biloba L.
Schmelzer et al. Medicinal plants 2
Jansen Dyes and tannins
Del Tredici The evolution, ecology, and cultivation of Ginkgo biloba
CN1256873A (en) Application of macleaya cordata in pesticide preparation
US20170280643A1 (en) Method of Improving the Growth and Production Output of Plants of the Family Solanaceae
Lemmens et al. Timbers 2
CN105613603B (en) A kind of organic peach vegetalitas black peach aphid insecticide and its application method
Pennington Tarahumar fish stupefaction plants
CN105660717B (en) A kind of organic water chestnut vegetable insecticide and its application method
Ky-Dembele et al. Clonal propagation of Pterocarpus santalinoides L'Hér. ex DC.: the effect of substrate, cutting type, genotype and auxin
Nellis Seashore plants of south Florida and the Caribbean: a guide to knowing and growing drought-and salt-tolerant plants
CN105010422A (en) Botanical rice planthopper repellent
CN105638752B (en) A kind of organic peach vegetalitas peach smaller green leaf hopper insecticide and its application method
Bhansali Biology and multiplication of Prosopis species grown in the Thar Desert
CN105660718B (en) A kind of organic lotus rhizome vegetable insecticide and its application method
Board Handbook On Spices (Reprint Edition-2010)
Essien et al. Industrial Crops Production
Williams Drug and condiment plants

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180406

Termination date: 20190226

CF01 Termination of patent right due to non-payment of annual fee