CN105901032A

CN105901032A - Organic loquat botanical phylloxera insecticide and application method thereof

Info

Publication number: CN105901032A
Application number: CN201610447445.3A
Authority: CN
Inventors: 郭兆武; 邵亚楠; 杨洁
Original assignee: Changsha University of Science and Technology
Current assignee: Changsha University of Science and Technology
Priority date: 2016-06-21
Filing date: 2016-06-21
Publication date: 2016-08-31
Anticipated expiration: 2036-06-21
Also published as: CN105901032B

Abstract

The invention discloses organic loquat botanical phylloxera insecticide and an application method thereof. The botanical insecticide is prepared by means of compatibility of fresh leaves of pinus massoniana, fresh fruits of seven-star pepper Szechuan, fresh stems and leaves of tripterygium wilfordii and human urine, wherein the weight-to-mass ratio of the fresh leaves of pinus massoniana to the fresh fruits of seven-star pepper Szechuan to the fresh stems and leaves of tripterygium wilfordii to the human urine is (2.5-3.5):(1.0-2.0):(3.5-4.5):(7.0-10.0), preferably the ratio is 3.0:1.5:4.0:8.5, the four components are calculated according to the weight-to-mass ratio, the fresh stems and leaves of tripterygium wilfordii serve as an auxiliary, and coordinating agents have a synergistic effect. The preparation has a remarkable killing effect on organic loquat phylloxera. The preparation is diluted with water to be sprayed according to the dosage of 1.0:2.0 per 40-60 kg.667 m<-2>. The preparation is stable in type, rich in raw material source, convenient to use during production, easy to operate, low in cost and free of chemical contamination, and completely meets the production requirement for organic loquats.

Description

A kind of organic Folium Eriobotryae vegetalitas wood louse insecticide and using method thereof

Technical field

The invention belongs to agricultural practical new technique research and development and application, the present invention relates to a kind of research and development and the production application method of organic Folium Eriobotryae vegetalitas wood louse insecticide.

Background technology

Folium EriobotryaeEriobotrya japonica(Thunb.) Lindl ］ it is Rosaceae, Eriobotrya, originate in southeast China, because leaf-shaped is gained the name like Chinese lute musical instrument, its flower can be used as medicine.Folium Eriobotryae is evergreen dungarunga, and height of tree 3-5m reaches as high as about 10m；Blade is big and grow, thickness and have fine hair, in oblong, shape such as Chinese lute, keratin, lanceolar, lanceolar, obovate or oval Long Circle, long 12-30cm, wide 3-9cm, tip is anxious sharp or tapering, base portion wedge shape or the narrowest one-tenth petiole, top edge has thin sawtooth, the full edge of base portion, light above, many wrinkles, below close raw taupe brown fine hair, lateral vein 11-21 pair；Petiole is short or a few stockless, long 6-10mm, has taupe brown fine hair；Stipule bores shape, long 1-1.5cm, the anxious point of tip, hairiness.Sprig is sturdy, yellowish-brown, close color or the taupe brown fine hair of getting rusty.Panicle top is raw, and long 10-19cm, tool is spent more；Total bennet and the close color fine hair that gets rusty of bennet, the long 2-8mm of bennet；Bract bores shape, long 2-5mm, the close color fine hair that gets rusty；Flower diameter 12-20mm；Calyx tube tazza shape, long 4-5mm, sepal triangular egg, long 2-3mm, the anxious point of tip, there is rust fine hair outside calyx tube and sepal；Petal white, Long Circle or avette, long 5-9mm, wide 4-6mm, base portion tool pawl, there is rust fine hair；Stamen 20, considerably shorter than petal, filigree base portion extends；Style 5, from life, stigma head, without hair, there are rust pubescence, Room 5 in ovary top, and there are 2 ovules every room.Fruit is spherical or Long Circle, diameter 2-5cm, yellow or orange colour, has outward rust pubescence, comes off soon；Seed 1-5, spherical or oblate spheroid, diameter 1-1.5cm, brown, light, seed coat papery.The month at florescence 10-12, really the phase 5-6 month.Ripe Folium Eriobotryae fruit also bunchy hang on the tree, the long 3-5cm of each fruit, circular, oval or long shape " Chinese lute shape ".Folium Eriobotryae surface is by villous, and dark green time underdone, stronger, aromatic odor is denseer.After maturation, crust is the most faint yellow, also has color relatively deep, close to orange-red.Sarcocarp is soft and succulence, can be divided mainly into white and orange two kinds.Different from major part fruit tree, Folium Eriobotryae is in autumn or early winter blooms, and fruit is ripe in spring to early summer, more Zao than other fruit, is therefore " solely for seasonal pathogens,exopathogens person in fruit tree " by title.Fruit soft and succulency, sweet and sour palatability, nutritious, leaf, flower, really, kernel can control cough.The tough and tensile exquisiteness of timber, is engraving, the high-quality timber making top-grade furniture.Folium Eriobotryae is mainly distributed on the ground such as Gansu, Shaanxi, Henan, Jiangsu, Anhui, Zhejiang, Jiangxi, Hubei, Hunan, Sichuan, Yunnan, Guizhou, Guangxi, Guangdong, Fujian, Taiwan.Various places wide row cultivation, there are wild species in Sichuan, Hubei.Also there are cultivation in Japan, India, Vietnam, Burma, Thailand, Indonesia.

Folium Eriobotryae is the raw economic plants of drought of a kind of high-yield and high-efficiency, and various places have cultivates widely, and along with planting site industry restructuring, its cultivated area has the gesture of continuous expansion.In recent years, Folium Eriobotryae pest and disease damage was on the rise, it has also become the big obstacle that development Fractus Eriobotryae industry produces.If Folium Eriobotryae insect pest mainly has Folium Eriobotryae wood louse first demodicid mite, aphid, yellow caterpillar, oriental fruit months, boat moth, Anoplophorae seu Aprionae etc., in recent years, the harm of Folium Eriobotryae wood louse is more universal, it has also become one of its Major Pests.

Folium Eriobotryae wood louse belongs to Homoptera Psyllidae, concentrates on young sprout with adult and nymph, petiole, blade suck juice.The distortion of killed blade vein, blade face shrinkage, produce withered speckle, and gradually blackening, induction sooty mould and fallen leaves.Wood louse occurs universal in recent years on Folium Eriobotryae, occurred in 1 year many generations, occurs heavier at arid season.After fruit picking, its position of causing harm is concentrated mainly in the crack of petiole base, secreting mucus, and anti-rain drop erosion power is strong.Worm source accumulates in a large number, the warm winter, the generation of high temperature and drought, beneficially wood louse, particularly has pear tree to mix up or the garden of interplanting, occurs even more serious, and there is the new slightly rate of worm in the serious orchard that is injured up to more than 80%.

At present the prevention and controls of Folium Eriobotryae wood louse insect pest is mainly had: 1. cultural control.Strengthen rich water quality management, never overdrying, overly moist, strengthen tree vigo(u)r, improve resistant to diseases and insects, remove fallen leaves in time, in conjunction with fertilising, suitable plowed soils, reduce disease pest source radix.Reduce pear tree and the catch cropping of loquat as far as possible, mix up or interplanting.Strengthening orchard management, cut off overstocked branch, light lower limb branch, prune off, short cut, be important to cut off sick branch after fruit picking, the growth forcing down prosperous branch makes it slowly grow, and to promote that taking out of summer shoot is sent out, lays the foundation for next year high yield；Control nitrogenous fertilizer, enrich phosphorus, potash fertilizer, accomplish Wang Shu not nitrogen fertilizer application；Doing irrigation and drainage system well, accomplish energy draining in rainy season, can pour water during drought, do not poured water in the 7-8 month in treelet garden, the most loose tree dish.2, physical control.Trapping lamp is installed in field, utilizes the adult pest such as light trap wood louse, yellow caterpillar, utilize yellow card's trap aphid.3. Biological control.Plant grass and stay grass, attract natural enemy, utilize the big generation of the natural pest controlling capability control insect of natural enemy.4. chemical prevention.Taking out the key that the periods such as tip phase are preventing and treatings, grasp the key link period, and applying pesticides is prevented and treated, and in the medication of low instar larvae phase, medicament can be selected for 20% 800-1200 times of liquid of wood louse emulsion for cleaning, the safe 8000 times of liquid of 25% Acker or 10% are pacified green precious 1500 times of liquid and prevented and treated.

Above prevention and controls is all effective, and some even has remarkable result, such as chemical prevention.But these prevention and controls also have its obvious shortcoming, such as: cultural control is time-consuming takes a lot of work, in the case of current rural area green grass or young crops strong labour power is few, problem is the most prominent；Physical control can only control population quantity to a certain extent, and when environmental condition is suitable, its insect pest is difficult to avoid completely；Biological control cost is high, and technical difficulty is big, and prevention effect is affected by environment bigger；Although chemical prevention is respond well, but it is big to pollute residual, does not meets organic production requirement.And this organic Folium Eriobotryae vegetalitas wood louse insecticide overcomes the above shortcoming, this organic Folium Eriobotryae vegetalitas wood louse insecticide raw material is taken from free of contamination plant and crop, natural decomposition is fast, residence time is short, easy to use, simple to operate, low cost, insecticidal effect is notable, without any chemical contamination, complies fully with the requirement of organic Loquat growth.

Summary of the invention

The purpose of the present invention aims to provide a kind of organic Folium Eriobotryae vegetalitas wood louse insecticide, and this insecticide is easy to use, simple to operate, low cost, and insecticidal effect is notable.Another object of the present invention aims to provide the preferably using method of above-mentioned organic Folium Eriobotryae vegetalitas wood louse insecticide.It is an object of the invention to be accomplished by:

The insecticide of the present invention by the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis is brewed forms, wherein the fresh stem and leaf of Radix Tripterygii Wilfordii is mainly used as auxiliary agent, acts the effect promoting its insecticidal effect；Urina Hominis is used as brewed solvent.Inventor studies discovery, adds the fresh stem and leaf of auxiliary agent Radix Tripterygii Wilfordii and can play good synergistic function.Need it is emphasized that, this fresh leaf of Pinus massoniana Lamb of organic Folium Eriobotryae vegetalitas wood louse insecticide, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.This organic Folium Eriobotryae vegetalitas wood louse method for producing insecticide is: first fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit, Radix Tripterygii Wilfordii fresh stem and leaf mass ratio by weight is put in a certain amount of Urina Hominis and is soaked during preparation.The fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of four components of Urina Hominis are 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0.The most preferred weight mass ratio of above four components is 3.0:1.5:4.0:8.5.The fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis are pressed fresh weight mass ratio and are calculated, and Urina Hominis is calculated by the weight mass ratio of 1～30d.Four components mass ratio by weight mixes, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, plastic bag sealing is packed, and can preserve 2-3.When using this organic Folium Eriobotryae vegetalitas wood louse insecticide to kill wood louse, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most.Parasite killing dosage, is typically watered dilution spraying with filtrate by 1.0:2.0, is all sprayed onto is advisable with leaf, the branch bar of worm strain, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most, can preferably kill wood louse.This insecticide produces upper easy to use, simple to operate, low cost, and insecticidal effect is notable, and the shelf-life is longer, packs with plastic bag sealing, can carry out factorial praluction, forms product.

The present invention selects the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit and the fresh stem and leaf of auxiliary agent Radix Tripterygii Wilfordii, brewed medium Urina Hominis, have employed the compatibility of Suitable weight mass ratio, is configured to commercial botanical pesticide, and it has significant insecticidal effect.The following is the comparative test of its insecticidal effect.

Organic Folium Eriobotryae vegetalitas wood louse insecticide of the present invention has no that the report of like product, the present invention are the result of the tests that inventor is drawn through experimental study for many years and production practices at present, and is verified in the production of long period, and effect is preferable.And the formula of the present invention also to have technology content higher, dosage form is more stable, abundant raw material source, easy and simple to handle, as developed into commodity, for producing organic Folium Eriobotryae, low price.The most easy to use, insecticidal effect is notable, and use cost is relatively low, is a kind of good botanical pesticide producing organic Folium Eriobotryae.Organic Folium Eriobotryae vegetalitas wood louse insecticidal formulation can carry out factorial praluction, it develops not only will commercially increase a kind of novel organic Folium Eriobotryae vegetalitas wood louse insecticide, also provide a new solution route by the problem difficult for the organic Folium Eriobotryae deinsectization of production at present.

The compound method of the present invention is: first shaked off by earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, plastic bag sealing is packed, and can preserve 2-3.

The using method of the present invention is: when using this organic Folium Eriobotryae vegetalitas wood louse insecticide to prevent and treat, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most.Parasite killing dosage, is typically watered dilution spraying with filtrate by 1.0:2.0, is all sprayed onto is advisable with leaf, the branch bar of worm strain, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most, wood louse can be carried out preferably killing action.Spray before being typically chosen at 11 in fine day morning or after at 15 in afternoon and be advisable, do not rain in 24h to be ensured after spraying or manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.Droplet is the finest, is limited being formed without relatively large water drop on plant, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.After spray medicine, in 20d, sylvan life can not carry out the mixed battalion of breeding.Testing inspection shows, uses this organic Folium Eriobotryae vegetalitas wood louse insecticide, and it is detected by the organic Folium Eriobotryae of commodity thus produced, and the main functional component of its botanical pesticide does not all detect.The following is inventor and realize the purpose of the present invention, constantly selected and select the comparative test of the insecticidal effect that the raw material of organic Folium Eriobotryae vegetalitas wood louse insecticidal formulation of the applicable present invention and relevant proportioning thereof done by test.

One pack system is tested

One pack system test mainly qualitative and quantitative test each component single effect test to organic Folium Eriobotryae wood louse killing action.

Materials and methods

1.1.1 Test material

The wood louse more universal to the harm of organic Loquat growth is selected in test.Test single dose is: the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, four components of Urina Hominis.Wherein the fresh stem and leaf of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.

Test method

The preparation of test material: the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.First earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii are shaked off during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb is 3.0:8.5 with the weight mass ratio of Urina Hominis, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.Qi xing paprika fresh fruit is 1.5 with the weight mass ratio of Urina Hominis: 8.5, the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and is not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.The fresh stem and leaf of Radix Tripterygii Wilfordii is 4.0:8.5 with the weight mass ratio of Urina Hominis, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.Urina Hominis, with the Urina Hominis of 1～30d, 25～50L big Plastic Drums are added a cover to deposit and are not sealed during 30d(deposits).By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, is all sprayed onto is advisable with leaf, the branch bar of worm strain, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays.1200 times of liquid sprayings of contrast chemical pesticide 20% wood louse emulsion for cleaning (its commodity cream contains Amitraz, phoxim and lambda-cyhalothrin), viewing test effect after 24h, not rain in ensureing 24h after spraying, period the most manually sprays.Test effect mainly counts and statistical analysis with the percentage ratio of its wood louse mortality rate.Spraying time is carried out before wood louse is broken out.

Orchard is tested: test arranges 5 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random distributed points.Numbered process 1-5 and CK1 of each process.

Laboratory test: test arranges 5 and processes 1 comparison, and each process 3 repetition (including CK), each repetition 1 basin (refers to that net has the basin of wood louse and Folium Eriobotryae fresh-cut branch and leaf), respectively processes random alignment.Numbered process 6-10 and CK2 of each process.

Each disposition is as follows:

The weight mass ratio processing the 1 fresh leaf of Pinus massoniana Lamb and Urina Hominis is 3.0:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.The weight mass ratio processing 2 qi xing paprika fresh fruits and Urina Hominis is 1.5:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2 Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.The weight mass ratio processing the 3 fresh stem and leaf of Radix Tripterygii Wilfordii and Urina Hominis is 4.0:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Processing the Urina Hominis that 4 with 1～30d, 25～50L big Plastic Drums are added a cover to deposit and are not sealed during 30d(deposits).By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed by this urine^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 5 and use 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK1) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.The weight mass ratio processing the 6 fresh leaves of Pinus massoniana Lamb and Urina Hominis is 3.0:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2 Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.The weight mass ratio processing 7 qi xing paprika fresh fruits and Urina Hominis is 1.5:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.The weight mass ratio processing the 8 fresh stem and leaf of Radix Tripterygii Wilfordii and Urina Hominis is 4.0:8.5, and the big Plastic Drum of Urina Hominis 25～50L with 1～30d is added a cover during immersion 30d(soaks and do not sealed), by 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed with this filtrate^-2 Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Processing the Urina Hominis that 9 with 1～30d, 25～50L big Plastic Drums are added a cover to deposit and are not sealed during 30d(deposits).By 3 layers of filtered through gauze after 30d, it is thus achieved that its filtrate.Plastic bag sealing packaging preserves 3 years.50kg.667m is pressed by this urine^-2 Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 10 and use 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2 Dosage spray, viewing test effect after 24h.Comparison (CK2) spray tap water 50kg.667m^-2Consumption, viewing test effect after 24h.

Statistical analysis

Test data is used SAS(6.12) carry out statistical analysis.Result of the test shows (table 1), and the process 1-3 of orchard test, the wood louse mortality rate of 5 are significantly higher than process 4 and CK1, and the wood louse mortality rate of process 4 is significantly higher than other with CK1 processes without significant difference, the wood louse mortality rate of process 5.Described above, the fresh leaf of Pinus massoniana Lamb of orchard test, qi xing paprika fresh fruit, the wood louse mortality rate of the fresh stem and leaf of Radix Tripterygii Wilfordii are all significantly higher than Urina Hominis and CK1, but the substantially less than process 5 of 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning, Urina Hominis processes the wood louse mortality rate with CK1 without significant difference.

Table 1. One pack system is testedThe mortality rate (unit: %) of wood louse

Process	Mortality rate	Process	Mortality rate
				Process 1	16.97±3.69b	Process 6	21.57±0.81c
Process 2	15.27±2.28bc	Process 7	23.91±1.69bc
				Process 3	13.91±4.09c	Process 8	2530±2.17b
Process 4	0.81±0.26d	Process 9	3.42±1.19d
				Process 5	96.04±6.83a	Process 10	98.27±3.28a
CK1	1.24±0.41d	CK2	2.64±0.81d

Note: lower case is 0.05 level, with string difference letter representation significant difference, lower same.

The process 6-8 of laboratory test, the wood louse mortality rate of 10 are significantly higher than process 9 and CK2, and the wood louse mortality rate processing 9 is significantly higher than other process with CK2 without significant difference, the wood louse mortality rate processing 10.Described above, the fresh leaf of Pinus massoniana Lamb of laboratory test, qi xing paprika fresh fruit, the wood louse mortality rate of the fresh stem and leaf of Radix Tripterygii Wilfordii are all significantly higher than Urina Hominis and CK2, but the substantially less than process 10 of 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning, Urina Hominis processes the wood louse mortality rate with CK2 without significant difference.

All shown with laboratory test by the test of above one pack system orchard, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the wood louse mortality rate of the fresh stem and leaf of Radix Tripterygii Wilfordii are all significantly higher than Urina Hominis and process and CK1, CK2, but the process of substantially less than 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning.Urina Hominis processes the mortality rate of CK1, the CK2 with spray tap water without significant difference.

Compatibility mechanism

Compatibility mechanism mainly qualitative and quantitative tests each ingredients to killing the composite effect test endangering the more universal wood louse of organic Folium Eriobotryae.

Materials and methods

2.1.1 Test material

The wood louse more universal to the harm of organic Loquat growth is selected in test.Test ingredients is the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, four components of Urina Hominis.Wherein the fresh stem and leaf of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.

Test method

The preparation of test material: the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.First earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii are shaked off during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution.Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, is all sprayed onto is advisable with leaf, the branch bar of worm strain, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays.1200 times of liquid sprayings of contrast chemical pesticide 20% wood louse emulsion for cleaning (its commodity cream contains Amitraz, phoxim and lambda-cyhalothrin), viewing test effect after 24h, not rain in ensureing 24h after spraying, period the most manually sprays.Test effect mainly counts and statistical analysis with the percentage ratio of its wood louse mortality rate.Spraying time is carried out before wood louse is broken out.

Orchard is tested: test arranges 4 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random distributed points.Numbered process 1-4 and CK1 of each process.

Laboratory test: test arranges 4 and processes 1 comparison, and each process 3 repetition (including CK), each repetition 1 basin (refers to that net has the basin of wood louse and Folium Eriobotryae fresh-cut branch and leaf), respectively processes random alignment.Numbered process 5-8 and CK2 of each process.

Each disposition is as follows:

Process the 1 fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5:1.0:3.5:7.0.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 2 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 3 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.5:2.0:4.5:10.0.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 4 and use 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK1) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.Process the 5 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5:1.0:3.5:7.0.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 6 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 7 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.5:2.0:4.5:10.0.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 8 and use 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK2) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.

Statistical analysis

Test data is used SAS(6.12) carry out statistical analysis.Result of the test shows (table 2), and each wood louse mortality rate processed that orchard is tested is significantly higher than CK1, and the wood louse mortality rate of process 3 and process 4, without significant difference, process 2 with process 3 without significant difference, process 2 and process 1 significant difference.Described above, the fresh leaf of Pinus massoniana Lamb of orchard test, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0, it is watered dilution spraying by 1.0:2.0 by this compatibility liquid stock solution, the effect of the orchard test after 24h is notable to the killing action of wood louse, with with the spraying of 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning, the test effect after 24h substantially close to.Wherein, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the orchard test effect that weight mass ratio is 3.0:1.5:4.0:8.5 of Urina Hominis four component are significantly better than the test effect that weight mass ratio is 2.5:1.0:3.5:7.0 of the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component.Combining insecticidal effect from economic benefit to consider, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the compatibility that weight mass ratio is 3.0:1.5:4.0:8.5 of Urina Hominis four component are the preferable ingredients killing Folium Eriobotryae wood louse.

Table 2. Compatibility mechanismThe mortality rate (unit: %) of wood louse

Process	Mortality rate	Process	Mortality rate
				Process 1	85.27±4.16c	Process 5	88.04±2.76c
Process 2	94.86±2.70b	Process 6	96.17±1.34b
				Process 3	95.27±1.81ab	Process 7	97.53±4.05ab
Process 4	97.16±2.07a	Process 8	99.51±2.42a
				CK1	0.72±0.31d	CK2	3.49±0.93d

Each wood louse mortality rate processed of laboratory test is significantly higher than CK2, and the wood louse mortality rate processing 6-8 is significantly higher than process 5, and the mortality rate between process 7-8, without significant difference, processes the mortality rate between 6-7 without significant difference.Described above, the fresh leaf of Pinus massoniana Lamb of laboratory test, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0, it is watered dilution spraying by 1.0:2.0 by this compatibility liquid stock solution, the effect of the laboratory test after 24h is notable to the killing action of wood louse, with with the spraying of 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning, the test effect after 24h substantially close to.Wherein, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the laboratory test effect that weight mass ratio is 3.0:1.5:4.0:8.5 of Urina Hominis four component is significantly better than the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the test effect that weight mass ratio is 2.5:1.0:3.5:7.0 of Urina Hominis four component, but leaf fresh with Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the effect that weight mass ratio is 3.5:2.0:4.5:10.0 of Urina Hominis four component is without significant difference, the effect that weight mass ratio is 3.5:2.0:4.5:10.0 of this four component is sprayed with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning again, test effect after 24h is without significant difference.Combining insecticidal effect from economic benefit to consider, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the compatibility that weight mass ratio is 3.0:1.5:4.0:8.5 of Urina Hominis four component are the preferable ingredients killing wood louse.

More than test is it is also shown that the wood louse mortality rate (table 2) of the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility test is significantly higher than the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the wood louse mortality rate (table 1) of Urina Hominis four component single dose test.It is indicated above that the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility have preferable synergistic function.

Dosetest

Dosetest mainly qualitative and quantitative tests each using dosage to killing the effect test endangering the more universal wood louse of organic Folium Eriobotryae.

Test material

Test method

The preparation of test material: the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.First earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii are shaked off during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution.Parasite killing dosage, is watered dilution spraying with filtrate by 1.0:2.0, is all sprayed onto is advisable with leaf, the branch bar of worm strain, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays.Contrast 1200 times of liquid sprayings of chemical pesticide 20% wood louse emulsion for cleaning, viewing test effect after 24h, not rain in ensureing 24h after spraying, period the most manually sprays.Test effect mainly counts and statistical analysis with the percentage ratio of its wood louse mortality rate.Spraying time is carried out before wood louse is broken out.

Each disposition is as follows:

Process the 1 fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.40kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 2 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 3 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.60kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 4 and press 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK1) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.Process the 5 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.40kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 6 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 7 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.60kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 8 and press 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK2) spray tap water 50kg.667m^-2Consumption, viewing test effect after 24h.

Statistical analysis

Test data is used SAS(6.12) carry out statistical analysis.Result of the test shows (table 3), and the wood louse mortality rate of the process 1-4 of orchard test is all remarkably higher than CK1.The wood louse mortality rate processing 2-4 is significantly higher than process 1, processes without significant difference between 3-4, processes between 2-3 without significant difference；Process 2 and be significantly higher than process 1.Described above, the fresh leaf of Pinus massoniana Lamb of orchard test, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5, press 50kg.667m by this compatibility liquid stock solution^-2With 60kg.667m^-2 Dosage 1.0:2.0 be watered dilution spraying, the orchard test effect after 24h is notable to the killing action of wood louse, without significant difference between the two.60kg.667m^-2Dosage 1.0:2.0 be watered dilution spraying with the spraying of 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning, the test effect observed after 24h is without significant difference.Combining insecticidal effect from economic benefit to consider, compatibility liquid stock solution presses 50kg.667m^-2 Dosage be the preferable dosage killing wood louse.

Table 3. DosetestThe mortality rate (unit: %) of wood louse

Process	Mortality rate	Process	Mortality rate
				Process 1	89.17±3.471c	Process 5	94.61±2.80b
Process 2	94.86±2.87b	Process 6	96.17±1.69b
				Process 3	95.82±0.83ab	Process 7	97.31±4.17ab
Process 4	97.16±2.49a	Process 8	99.51±1.94a
				CK1	0.72±0.27d	CK2	3.49±1.10c

The wood louse mortality rate of the process 5-8 of laboratory test is all remarkably higher than CK2.The wood louse mortality rate processing 7-8 is significantly higher than process 5, processes without significant difference between 7-8, processes between 5-7 without significant difference.Described above, the fresh leaf of Pinus massoniana Lamb of laboratory test, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5, press 40-60kg.667m by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying, the laboratory test effect after 24h is notable to the killing action of wood louse, 60kg.667m^-2 Dosage 1.0:2.0 be watered dilution spraying, the laboratory test effect after 24h is to the killing action of wood louse with the 1200 times of liquid sprayings of chemical pesticide 20% wood louse emulsion for cleaning, and the test effect observed after 24h is without significant difference.Wherein, compatibility liquid stock solution presses 50kg.667m^-2The laboratory test effect of dosage is substantially better than compatibility liquid stock solution by 40kg.667m^-2The test effect of dosage, but press 60kg.667m with compatibility liquid stock solution^-2The test effect of dosage is without significant difference.Combining insecticidal effect from economic benefit to consider, compatibility liquid stock solution presses 50kg.667m^-2Dosage be the preferable dosage killing wood louse.

More than test is it is also shown that the insecticidal effect i.e. mortality rate of wood louse (table 2-3) of the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility test is significantly higher than the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the mortality rate (table 1) of Urina Hominis four component single dose test.It is indicated above that the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component compatibility have preferable synergistic function.

Auxiliary agent is tested

Whole ingredients is killed the synergistic function of wood louse by auxiliary agent test mainly qualitative and quantitative test auxiliary agent.

Materials and methods

4.1.1 Test material

Test method

The preparation of test material: the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.First earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii are shaked off during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, plastic bag sealing pack, with preserve 3 years botanical pesticide for test insecticide stock solution.Parasite killing dosage, uses filtrate 50kg.667m^-2It is watered dilution spraying by 1.0:2.0.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays.Contrast 1200 times of liquid sprayings of chemical pesticide 20% wood louse emulsion for cleaning, viewing test effect after 24h, not rain in ensureing 24h after spraying, period the most manually sprays.Test effect mainly counts and statistical analysis with the percentage ratio of its wood louse mortality rate.Spraying time is carried out before wood louse is broken out.

Orchard is tested: test arranges 3 and processes 1 comparison, each process 3 repetition (including CK), each repetition 1 strain, respectively processes random distributed points.Numbered process 1-3 and CK1 of each process.

Laboratory test: test arranges 3 and processes 1 comparison, and each process 3 repetition (including CK), each repetition 1 basin (refers to that net has the basin of wood louse and Folium Eriobotryae fresh-cut branch and leaf), respectively processes random alignment.Numbered process 4-6 and CK2 of each process.

Each disposition is as follows:

Process the 1 fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 2 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the weight mass ratio of Urina Hominis three component are 3.0:1.5:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 3 and press 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK1) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.Process the 4 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process the 5 fresh leaves of Pinus massoniana Lamb, qi xing paprika fresh fruit, the weight mass ratio of Urina Hominis three component are 3.0:1.5:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.Viewing test effect after 24h.Process 6 and press 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray, viewing test effect after 24h.Comparison (CK2) spray tap water 50kg.667m^-2 Consumption, viewing test effect after 24h.

Statistical analysis

Test data is used SAS(6.12) carry out statistical analysis.Result of the test shows (table 4), and the wood louse mortality rate of the process 1-3 of orchard test is significantly higher than CK1, and the wood louse mortality rate processing 2 is substantially less than process 1,3, but is significantly higher than CK1.Described above, be 3.0:1.5 without the fresh leaf of compatibility Pinus massoniana Lamb of auxiliary agent, qi xing paprika fresh fruit, the weight mass ratio of Urina Hominis three component: 8.5, press 50kg.667m by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying, the mortality rate of wood louse is substantially less than had the fresh leaf of compatibility Pinus massoniana Lamb of auxiliary agent, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component to be 3.0:1.5:4.0:8.5 by the effect of the orchard test after 24h, presses 50kg.667m by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying, after 24h orchard test mortality rate；It is also significantly lower than with 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray 24h after orchard test mortality rate.

Table 4. Auxiliary agent is testedThe mortality rate (unit: %) of wood louse

Process	Mortality rate	Process	Mortality rate
				Process 1	94.86±2.37b	Process 4	96.17±2.04b
Process 2	47.39±6.43c	Process 5	55.82±1.90c
				Process 3	97.16±2.18a	Process 6	99.51±2.13a
CK1	0.72±0.21d	CK2	3.49±1.29d

The wood louse mortality rate of the process 4-6 of laboratory test is significantly higher than CK2, and the wood louse mortality rate processing 5 is substantially less than process 4,6, but is significantly higher than CK2.Described above, it is 3.0:1.5:8.5 without the fresh leaf of compatibility Pinus massoniana Lamb of auxiliary agent, qi xing paprika fresh fruit, the weight mass ratio of Urina Hominis three component, presses 50kg.667m by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying, the mortality rate of wood louse is substantially less than had the fresh leaf of compatibility Pinus massoniana Lamb of auxiliary agent, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component to be 3.0:1.5:4.0:8.5 by the effect of the laboratory test after 24h, presses 50kg.667m by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying, the mortality rate of laboratory test after 24h；It is also significantly lower than with 1200 times of liquid 50kg.667m of chemical pesticide 20% wood louse emulsion for cleaning^-2Dosage spray 24h after the mortality rate of laboratory test.

More than test shows (table 4), and adding assistant is notable with the difference on effect of not adding assistant, and the effect of adding assistant is significantly better than the effect of not adding assistant, and its effect of adding assistant does not significantly reduces.Illustrate that the fresh stem and leaf of auxiliary agent Radix Tripterygii Wilfordii has the most collaborative and potentiation in this compatibility.

Test is it is also shown that the effect (table 3-4) of ingredients is about 1-4 times of each single dose effect (table 1).So, the effect after compatibility is not the addition of simple each single dose effect.From single dose be tested to compatibility mechanism again to auxiliary agent test (table 1-4), result shows, the fresh leaf of compatibility Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis four component the compatibility liquid stock solution that weight mass ratio is 3.0:1.5:4.0:8.5 by 50kg.667m^-2Dosage 1.0:2.0 be watered the ingredients of dilution spraying, combine insecticidal effect from economic benefit and consider it is preferably to select.Test shows, sprays with the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the compatibility agent of Urina Hominis four component in the commodity Folium Eriobotryae of rear 30d and does not detects the compositions such as its main component larch turpentine, luteolin, capsaicin, wilfordine.

Hold concurrently action of contace poison for stomach poison for this brewed dose, its toxic component larch turpentine, luteolin, capsaicin, wilfordine etc. together enter its polypide metabolism of its internal upset with wood louse when taking food causes and is poisoned to death, or directly cause internal metabolism chaotic in polypide body surface pore intrusion wood louse body and cause and be poisoned to death, or cause joint paralysis internal metabolism chaotic from polypide without the intrusion of waxiness the most cornified joint cell gap and finally result in death.

Pinus massoniana Lamb (Pinus massoniana Lamb.) it is Pinaceae pine genus plant, arbor, high up to 45m, diameter of a cross-section of a tree trunk 1.3 meters above the ground 1.5m；Bark bronzing, bottom taupe, it is cleaved into irregular squamous block sheet；Branch is open and flat or tiltedly opens up, tree crown width turriform or umbrella shape, and branch grows every year takes turns, but the most generally grows two-wheeled at South Guangdong, khaki, and without white lead, rare white lead, without hair；Hibernaculum ovum shape is cylindrical or cylindrical, brown, top point, and perula edge is thread, tip point or become tapering long tip, micro-contrary flexure.Needle 2 pin is a branch of, and dilute 3 pins are a branch of, and long 12-20cm is fine and soft, micro-distortion, and two sides has spiracle line, edge to have serration；Cross section hypodermic layer cell monotype, ground floor continuous arrangement, the second layer is formed by respective cells intermittent arrangement, resin canal about 4-8, and limit is raw overleaf, or the outside of belly also has 2 limits raw；Sheath is just the most brown, after crossfade into grey black, harbor.Male cone reddish tan, cylindrical, curved vertical, long 1-1.5cm, consor is in shoot bottom bud axil, spike, long 6-15cm；Female cone is single raw or 2-4 consor is in the nearly top of shoot, lilac red, annual conelet spheroidal or oval, footpath about 2cm, brown or puce, and the squama umbilicus of top cone-scale has the most upright short thorn, and the squama umbilicus of bottom cone-scale is put down blunt stingless.Cone oval or coniform oval, long 4-7 Cm, footpath 2.5-4 cm, have short stalk, sagging, ripe front green, and Chestnut time ripe comes off successively；Middle part fruit scale nearly square round shape obovate, or nearly rectangle, be about 3 cm；Scutum rhombus, micro-protuberance or flat, transverse ridge is slightly bright aobvious, and squama umbilicus nick, stingless, person often has extremely short thorn to be born in dry environment；The long oval of seed, long 4-6mm, Fructus Forsythiae long 2-2.7 cm；Cotyledon 5-8 piece；Long 1.2-2.4 cm；Acrospire bar shaped, long 2.5-3.6 Cm, leaf margin tool dredges raw setiform sawtooth.The month at florescence 4-5, the cone Second Year 10-12 month is ripe.Pinus massoniana Lamb distributed pole is wide, north from Henan and Southern Shandong Province, south to Guangdong and Guangxi Provinces, Hunan, Taiwan, east, from coastal, west to central Sichuan and Guizhou, is dispersed throughout various places, south China, Central China.General at THE LOWER YANGTZE VALLEY height above sea level 600～below 700m, middle reaches about more than 1200m, upstream about below 1500m all have distribution, are SOUTHERN CHINA main material seeds, economic worth height.Pinus massoniana master is containing secondary metabolitess such as larch turpentine, luteolin, Quercetins.

Fructus Capsici (Capsicum annuum L.) it is Solanaceae, Capsicum 1 year or limited herbaceos perennial.Domestic Fructus Capsici principal item has qi xing paprika、Gold green pepper, sky, sea green pepper etc..Caulis Capsici is near without hair or micro-raw pubescence, branch warpage slightly in a zigzag.Leaf alternate, branch top joint does not extend and forms twin or bunch raw shape, and square round shape is avette, avette or ovum shape lanceolar, Quan Yuan, the short tapering or anxious point in top, the narrow wedge shape of base portion；Flower Dan Sheng, bows vertical；Calyx cup-shaped, the most notable 5 teeth；Corolla white, sliver is avette；Flower pesticide gray purple.Carpopodium is more sturdy, bows vertical；The long finger-like of fruit, top is tapering and often bends, green during immaturity, becomes red, orange or aubergine after maturation, and taste is peppery.Seed flat kidney shape, faint yellow.Pepper fruit mainly contains alkaloid, pigment, oils, wherein, Fructus Capsici alkaloid is the piquancy component of Fructus Capsici, mainly includes: capsaicin (Capsaicin), dihydrocapsaicin (Dihydrocapsaicin), Nordihydrocapsaicin (Nordihydrocapsaicin), homocapsaicin (Homocapsaicin), Homodihydrocapsaicin (Homodihydrocapsaicin) and trace element, vegetable protein etc.；In Fructus Capsici, contained pigment composition is mainly capsicum red pigment (also referred to as capsanthin), capsorubin etc.；Containing Solanidine (solatubine) etc. in pepper seed, also have containing cryptoxanthine (cryptoxenthin), carotene (carotene), vitamin C, citric acid, tartaric acid, malic acid etc..

Radix Tripterygii Wilfordii (Tripterygium wilfordii Hook. f.), Celastraceae, tripterygium plant, liana shrub, main product is in China, Korea, Japan, and China is distributed mainly on various places and southwest on the south the Yangtze river basin, main product in Fujian, Zhejiang, Hubei, Hunan, Anhui, the ground such as Henan.In being born in the shade how wet hillside, mountain valley, small stream limit bushes.The hillside that happiness is more shady and cool, the sand soil deep with slant acidity, soil layer or yellow soil the most preferably grow.Bitter in the mouth, pungent, cool in nature, poison greatly.Thunder God Calamus liana shrub, high 1～3m, sprig brownish red, there are 4～6 thin ribs, by Mi Mao and fine and closely woven hole skin.Elliptic leaf shape, the ovum ellipse or avette oval, rectangular that falls, long 4～7.5cm, wide 3～4cm, tip is anxious sharp or short tapering, and the wealthy wedge shape of base portion or circle, there is serration at edge, and lateral vein 4～7 is right, slightly goes up curved after reaching leaf margin；Petiole length 5～8mm, close by rust hair.Circular cone cyme is narrower, long 5～7cm, wide 3-4cm, generally has 3～5 branches.The long round shape of Myriopteron extensum (Wight) K. Schum, long 1～1.5cm, diameter 1～1.2cm, seed buttress shaft shape, up to 10mm.Radix Tripterygii Wilfordii is mainly containing wilfordine, Celastrus orbiculatus Thunb. benzyl amide, Triptolide etc..

Detailed description of the invention

Implementation below and embodiment are intended to further illustrate the present invention rather than limitation of the present invention.

Embodiment 1 :

The preparation of material: the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.Otherwise, the quality of organic Folium Eriobotryae will be affected.First earth, water and other foreign bodies etc. on fresh for Pinus massoniana Lamb leaf, qi xing paprika fresh fruit and the fresh stem and leaf of Radix Tripterygii Wilfordii are shaked off during preparation, do not need washing, if band dew etc. when gathering, the preparation of material is carried out again after water to be treated is dry, qi xing paprika fresh fruit is smashed to pieces into irregular graininess or bulk (size is without being strict with), and the fresh leaf of Pinus massoniana Lamb and the fresh stem and leaf of Radix Tripterygii Wilfordii are cut into the segment of 3-5cm length.Thereafter, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis mass ratio by weight mix, 25～50L big Plastic Drums do not seal during adding a cover immersion 20～40d(immersion), by 3～4 layers of filtered through gauze after 20～40d, its filtrate is this organic Folium Eriobotryae vegetalitas wood louse insecticide, and plastic bag sealing is packed, and can preserve 2～3 years.

Selection of time is used to spray medicine before organic Folium Eriobotryae wood louse is broken out, it is possible to carry out early prevention.During use, use filtrate 40kg.667m^-2It is watered dilution spraying by 1.0:2.0.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.Droplet is the finest, be formed without on plant relatively large water drop for degree, safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.After spray medicine, in 20d, sylvan life can not carry out the mixed battalion of breeding.

The fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.40kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.After 24h, the insecticidal effect in organic Folium Eriobotryae orchard is: wood louse mortality rate 89.17%, presses 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2The insecticidal effect of dosage spray only differ 7.99%, substantially close to the insecticidal effect of chemical pesticide 20% 1200 times of liquid of wood louse emulsion for cleaning.

Embodiment 2 :

Selection of time is used to spray medicine before organic Folium Eriobotryae wood louse is broken out, it is possible to carry out early prevention.During use, use filtrate 50kg.667m^-2It is watered dilution spraying by 1.0:2.0.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.Droplet is the finest, be formed without on plant relatively large water drop for degree, safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.After spray medicine, in 20d, sylvan life can not carry out the mixed battalion of breeding.

The fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.50kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.After 24h, the insecticidal effect in organic Folium Eriobotryae orchard is: wood louse mortality rate 94.86%, presses 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2The insecticidal effect of dosage spray only differ 2.30%, substantially close to the insecticidal effect of chemical pesticide 20% 1200 times of liquid of wood louse emulsion for cleaning.

Embodiment 3 :

Selection of time is used to spray medicine before organic Folium Eriobotryae wood louse is broken out, it is possible to carry out early prevention.During use, use filtrate 60kg.667m^-2It is watered dilution spraying by 1.0:2.0.Selecting to spray before at 11 in fine day morning or after at 15 in afternoon, do not rain after spraying in guarantee 24h, period the most manually sprays, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.Droplet is the finest, be formed without on plant relatively large water drop for degree, safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.After spray medicine, in 20d, sylvan life can not carry out the mixed battalion of breeding.

The fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, the weight mass ratio of Urina Hominis four component are 3.0:1.5:4.0:8.5.60kg.667m is pressed by this compatibility liquid stock solution^-2Dosage 1.0:2.0 be watered dilution spraying.After 24h, the insecticidal effect in organic Folium Eriobotryae orchard is: wood louse mortality rate 95.82%, presses 50kg.667m with 1200 times of liquid of chemical pesticide 20% wood louse emulsion for cleaning^-2The insecticidal effect of dosage spray only differ 1.34%, substantially close to the insecticidal effect of chemical pesticide 20% 1200 times of liquid of wood louse emulsion for cleaning.

Claims

1. an organic Folium Eriobotryae vegetalitas wood louse insecticide and using method thereof, it is characterized in that, described organic Folium Eriobotryae vegetalitas wood louse insecticide by the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, Radix Tripterygii Wilfordii fresh stem and leaf compatibility Urina Hominis is brewed forms, wherein the fresh stem and leaf of Radix Tripterygii Wilfordii is auxiliary agent, and Urina Hominis is brewed medium.

One the most according to claim 1 organic Folium Eriobotryae vegetalitas wood louse insecticide, it is characterized in that, the compatibility weight mass ratio of the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of auxiliary agent Radix Tripterygii Wilfordii and brewed medium Urina Hominis is 2.5～3.5:1.0～2.0:3.5～4.5:7.0～10.0.

3. according to the one organic Folium Eriobotryae vegetalitas wood louse insecticide described in claim 1 and 2, it is characterised in that the preferred weight mass ratio of the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii and Urina Hominis is 3.0:1.5:4.0:8.5.

4. according to the organic Folium Eriobotryae vegetalitas wood louse insecticide described in claim 2 to 3, it is characterized in that, the fresh leaf of Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii are all by the weight Mass Calculation of fresh material, and Urina Hominis presses the weight Mass Calculation of 1～30d, soaking 20～40d, brewed period is not required to seal.

5. according to the organic Folium Eriobotryae vegetalitas wood louse insecticide described in claim 2 to 4, it is characterized in that, the fresh leaf of material Pinus massoniana Lamb, qi xing paprika fresh fruit, the fresh stem and leaf of Radix Tripterygii Wilfordii, Urina Hominis all should be selected from environmental conditions such as soil, water, air pollution-free, its growing environment did not used the area of chemical fertilizer, chemical pesticide, without industrial pollution and the heavy metal pollution such as opening a mine.

6. according to the organic Folium Eriobotryae vegetalitas wood louse insecticide described in claim 2 to 4, it is characterized in that, during use, need to ensure that all sites such as leaf, branch bar has been sprayed onto position the most, spray before being typically chosen at 11 in fine day morning or after at 15 in afternoon and be advisable, do not rain in 24h to be ensured after spraying or manually spray, if having rained in 24h or having carried out artificial sprinkling irrigation, need to again spray.

Using method the most according to claim 6, is characterized in that, selects to spray medicine before wood louse population number does not has great outburst, by 40～60kg.667m^-2Dosage 1.0:2.0 be watered dilution spraying, droplet is the finest, is limited being formed without relatively large water drop on plant, the wood louse of organic Folium Eriobotryae can be carried out effective killing action.

8. according to the using method described in claim 6-7, it is characterized in that, all can use it can also be used to prevent in Folium Eriobotryae young sprout emergence period or wood louse harm phase, after spray medicine, in 20d, sylvan life can not carry out the mixed battalion of breeding, and safe defence strictly to carry out human body defence and operation by chemical pesticide rule of operation.