CN104642398B - The purposes of southern biennial wormwood extract preventing and treating Alternaria panax - Google Patents
The purposes of southern biennial wormwood extract preventing and treating Alternaria panax Download PDFInfo
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- CN104642398B CN104642398B CN201510035054.6A CN201510035054A CN104642398B CN 104642398 B CN104642398 B CN 104642398B CN 201510035054 A CN201510035054 A CN 201510035054A CN 104642398 B CN104642398 B CN 104642398B
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Abstract
The invention discloses the purposes of southern biennial wormwood extract preventing and treating Alternaria panax, belong to plant pest management technical field, the preparation method of the southern biennial wormwood extract is as follows：(1) the clean rear naturally dry of disease-free southern argy wormwood stem, the fresh body of leaf is chosen, fresh body grinding and sieving, then stem, leaf powder is mixed and is placed in drying receptacle；(2) toward addition ethanol progress first time filtering in southern argy wormwood powder obtained by step (1), filtrate is obtained, gained filter residue is added into ethanol filter for the second time, after repetition is filtered 2 times, merges 3 filtrates；(3) it is then to obtain the southern biennial wormwood extract of the present invention with sterilized water constant volume using ultraviolet sterilization by filtrate obtained by step (2) through being concentrated under reduced pressure.The southern biennial wormwood extract of the present invention has good fungistatic effect to notoginseng root rot pathogen.
Description
Technical field
The present invention relates to techniques of pest control field, more particularly to the use of southern biennial wormwood extract preventing and treating Alternaria panax
On the way.
Background technology
Southern argy wormwood (Artemisia verlotorum Lamotte), belongs to composite family artemisia, nickname Artemisia santonica, Artemisia stelleriana.Due to
Sagebruss is similar on profile, smell, also may be collectively referred to herein as " wormwood " or " argy wormwood ", big portion of China, and Europe, Asia and Africa etc. is all
It is distributed.This plant is adopted to be perennial, plant has fragrance.Main root is slightly obvious, and lateral root is more.Stem list is raw or a small number of, high 50-100
Centimetre, branch is long 5-8 centimetres, is affixed to stem obliquely.It is seen everywhere in the wild, important ground is occupied in traditional Chinese culture
Position.It can be used as medicine, there is anti-inflammatory, anastalsis.
Pseudo-ginseng (Panax notoginseng F.H.chen) also known as pseudo-ginseng, invaluable etc., have it is significant it is promoting blood circulation and removing blood stasis,
Detumescence ding-tong effect, it is a kind of Chinese tradition rare medicinal herbs.Using pseudo-ginseng as Chinese patent drug made of primary raw material such as " Yunnan Baiyao "
" Pien Tze Huang " etc. is wide-spread.In recent years, it is growing to the demand of pseudo-ginseng raw material.But the disease in cultivation is tight
Panax notoginseng Growth is have impact on again, and the abuse of chemical pesticide also have impact on the quality of pseudo-ginseng.Alternaria panax is pseudo-ginseng Major Diseases
One of, Alternaria panax can infect pseudo-ginseng plant and underground root system, aggrieved heavier with stem, leaf, floral axis.It is brown after root infection
Web rot shape, the four seasons can cause the generation of root-rot.Pseudo-ginseng garden temperature of shed is high, high humidity, and improper fertilization is likely to make disease
Sprawling aggravates.The general long-term incidence of disease is up to more than 60% between 5%-20% when serious.Its symptom is mostly by pseudo-ginseng blackspot
Caused by germ (Alternaria panax Whetzel) harm.Alternaria panax is to cause pseudo-ginseng product quality decline in recent years
The main reason for.
The southern biennial wormwood extract of the present invention has good to Alternaria panax opportunistic pathogen (Alternaria panax Whetzel)
Good fungistatic effect, this bacteriostasis have positive application value, have weight to the biological prevention of Alternaria panax
Want meaning.
The content of the invention
Present invention aims at provide a kind of purposes of southern biennial wormwood extract preventing and treating Alternaria panax.It is provided by the present invention
Technical scheme is：
The invention provides the purposes of southern biennial wormwood extract preventing and treating Alternaria panax.
In addition, present invention also offers the preparation method of southern biennial wormwood extract, it is comprised the following steps that：
(1) disease-free southern argy wormwood stem is chosen, the fresh body of leaf cleans rear naturally dry, fresh body grinding and sieving, then by stem, leaf
Powder mixing is placed in drying receptacle；
(2) first time filtering is carried out toward addition ethanol in southern argy wormwood powder obtained by step (1), obtains filtrate, gained is filtered
Slag adds ethanol and carries out second of filtering, repeats after filtering 2 times, merges 3 filtrates；
(3) by filtrate obtained by step (2) through being concentrated under reduced pressure, using ultraviolet sterilization, then obtained with sterilized water constant volume
The southern biennial wormwood extract of the present invention.
In technical solution of the present invention, sieving described in step (1) uses the aperture of sieve as 50 mesh.
In technical solution of the present invention, step filters for the first time described in (2), is that 8 times of amount second are added in southern argy wormwood powder
Alcohol soaks 4d at room temperature, then with four layers of filtered through gauze, obtains filtrate.
Second of filtering described in step (2), it is that will filter gained filter residue first time with 6 times in technical solution of the present invention
Amount ethanol soaks 4d at room temperature, then with four layers of filtered through gauze, obtains filtrate.
In technical solution of the present invention, the concentration of step (3) Central-South biennial wormwood extract is 2.0gmL-1。
Compared with prior art, the device have the advantages that being：
The southern biennial wormwood extract of the present invention can substantially suppress Alternaria panax opportunistic pathogen, and its inhibitory action is with crude drug concentration
Reduction, incubation time extension and decline.
Embodiment
With reference to specific embodiment, make further details of elaboration to the present invention, but embodiments of the present invention are not
It is confined to the scope of embodiment expression.
Embodiment 1：The preparation of southern biennial wormwood extract
Choose disease-free southern argy wormwood stem, the fresh body of leaf cleans rear naturally dry.Fresh body crushed the sieve that aperture is 50 mesh, so
Its stem, leaf powder are mixed and be placed in drying receptacle afterwards, 8 times of amount ethanol is added and soaks 4d at room temperature, then with four layers of gauze mistake
Filter, obtains filtrate.Filter residue repeats previous step dip operation with 6 times of amount ethanol, after being repeated 2 times, merges filtrate three times.Filtrate subtracts
Pressure, concentration, after ultraviolet sterilization, it is 2.0gmL to be then settled to crude drug concentration with sterilized water-1。
Embodiment 2：The bacteriostasis of southern biennial wormwood extract
2.1 materials and reagent
Southern argy wormwood ethanol extract, sterilized water, PDA culture medium.For trying strain：Alternaria panax opportunistic pathogen (Alternaria
Panax Whetzel) provided by agricultural college of Guangxi University plant pathology laboratory.
2.2 method
The fungistatic effect of southern biennial wormwood extract is determined using mycelial growth rate method.It is 2.0gmL to measure crude drug concentration-1
Southern argy wormwood leaching liquor 6.75ml, 3.375ml, 1.6875ml, be settled to 10ml with sterilized water respectively, be made various concentrations south
Argy wormwood test solution, the southern argy wormwood test liquid 1ml of various concentrations is taken respectively, add the PDA culture medium 14ml melted in 90mm
Drug containing flat board is made in culture dish, it is finally 0.0900gmL for examination concentration to make it-1、0.0450g·mL-1And 0.0225g
mL-1, to add equivalent sterilized water as control.After culture medium solidifying, beaten and taken with card punch in the pathogen species cultivated in advance
Diameter 6mm mycelia blocks, the center of culture dish is transferred to, one piece is inoculated with per culture dish, 28 DEG C of culture 96h, each for examination concentration weight
Again three times.In the 48th, 72h, 96h using crossing method measurement colony diameter, the average result repeated three times is taken, obtains difference
Concentration extract is to the inhibition for thing bacterium.Following formula calculate the inhibiting rate to pathogen：
Bacteriostasis rate (%)=[(the pure increment of the control bacterium colony-pure increment of processing bacterium colony)/pure increment of control bacterium colony] ×
100%；Pure increment=bacterium colony average diameter-bacteria cake diameter
Inhibitory action of the southern biennial wormwood extract of the various concentrations of table 1 to Alternaria panax pathogen
As shown in Table 1, it is 0.0900gmL in southern biennial wormwood extract crude drug concentration-1Inhibiting rate is up to when cultivating 48h
It can also can exceed that 50% reaches 64.39% after 75.93%, 96h, it is seen that southern biennial wormwood extract can substantially suppress Alternaria panax original
The growth of bacterium (Alternaria panax Whetzel), its inhibitory action are prolonged with the reduction of crude drug concentration, incubation time
Grow and reduce.
The description of the foregoing specific illustrative embodiment to the present invention is to illustrate and the purpose of illustration.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (5)
1. the purposes of southern biennial wormwood extract preventing and treating Alternaria panax, it is characterised in that the preparation method of the southern biennial wormwood extract,
Comprise the following steps that：
(1) disease-free southern argy wormwood stem is chosen, the fresh body of leaf cleans rear naturally dry, fresh body grinding and sieving, then by stem, leaf powder
Mixing is placed in drying receptacle；
(2) first time filtering is carried out toward addition ethanol in southern argy wormwood powder obtained by step (1), obtains filtrate, by gained filter residue again
Addition ethanol carries out second and filtered, and repeats after filtering 2 times, merges 3 filtrates；
(3) by filtrate obtained by step (2) through being concentrated under reduced pressure, using ultraviolet sterilization, then this hair is obtained with sterilized water constant volume
Bright southern biennial wormwood extract.
2. purposes as claimed in claim 1, it is characterised in that：Sieving described in step (1) uses the aperture of sieve as 50 mesh.
3. purposes as claimed in claim 1, it is characterised in that：Step filters for the first time described in (2), is in southern argy wormwood powder
It is middle to add 8 times of amount ethanol and soak 4d at room temperature, then with four layers of filtered through gauze, obtain filtrate.
4. purposes as claimed in claim 1, it is characterised in that：Second of filtering described in step (2), it is to filter first time
Gained filter residue soaks 4d at room temperature with 6 times of amount ethanol, then with four layers of filtered through gauze, obtains filtrate.
5. purposes as claimed in claim 1, it is characterised in that：The concentration of step (3) Central-South biennial wormwood extract is 2.0gmL-1。
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| CN201510035054.6A CN104642398B (en) | 2015-01-23 | 2015-01-23 | The purposes of southern biennial wormwood extract preventing and treating Alternaria panax |
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| CN201510035054.6A CN104642398B (en) | 2015-01-23 | 2015-01-23 | The purposes of southern biennial wormwood extract preventing and treating Alternaria panax |
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| CN104642398B true CN104642398B (en) | 2017-11-17 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105409654B (en) * | 2015-11-04 | 2018-10-23 | 云南中医学院 | A method of covering substrate inhibition notoginseng root rot |
| CN105359897B (en) * | 2015-11-27 | 2018-08-14 | 安徽科技学院 | A method of utilizing Chinese mugwort cigarette fumigation control stem of noble dendrobium Seedling Diseases |
| CN105494334B (en) * | 2015-12-08 | 2018-03-13 | 北京农学院 | A kind of environmentally friendly bactericide for preventing and treating fruit tree bacterial disease and preparation method thereof |
| CN106342920A (en) * | 2016-08-25 | 2017-01-25 | 文山苗乡三七科技有限公司 | Method for preventing and controlling disease damage and pest damage of pseudo-ginseng by virtue of no- residue farm chemicals |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126615A (en) * | 2014-07-30 | 2014-11-05 | 广西大学 | Application of passiflora edulis extractive to control panax notoginseng black spot |
-
2015
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126615A (en) * | 2014-07-30 | 2014-11-05 | 广西大学 | Application of passiflora edulis extractive to control panax notoginseng black spot |
Non-Patent Citations (4)
| Title |
|---|
| "5种中药提取物对人参黑斑病菌的抑制作用";孙立晨 等，;《农药》;20080723;第47卷(第6期);全文 * |
| "艾蒿提取液对9种植物病原真菌抑制作用研究";桑娟萍 等，;《林业实用技术》;20120929;第2012卷(第5期);第1页正文部分1.4.1节 * |
| "艾蒿提取物对13种植物病原菌物的抑制作用";江茂生 等，;《福建农林大学学报（自然科学版）》;20071126;第36卷(第4期);第1页正文部分1.1.1节 * |
| "野艾蒿抑菌成分提取工艺的研究";陈海燕 等，;《食品工业科技》;20150113;第35卷(第23期);全文 * |
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