CN104126615A - Application of passiflora edulis extractive to control panax notoginseng black spot - Google Patents
Application of passiflora edulis extractive to control panax notoginseng black spot Download PDFInfo
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- CN104126615A CN104126615A CN201410369959.2A CN201410369959A CN104126615A CN 104126615 A CN104126615 A CN 104126615A CN 201410369959 A CN201410369959 A CN 201410369959A CN 104126615 A CN104126615 A CN 104126615A
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Abstract
The invention discloses application of passiflora edulis extractive to control panax notoginseng black spot, and belongs to the technical field of plant pest control. A preparation method for the passiflora edulis extractive comprises: (1) selecting disease-free pest-free passiflora edulis fresh body, cleaning, airing, cutting the fresh stem into small segments and shearing fresh leaves into fragments; and (2) adding water or absolute ethanol with the usage amount being 5-10 times into passiflora edulis fresh small segments and fresh fragments obtained in the step (1), extracting for 0.5-2 h at room temperature, performing ultrasonic processing for 20-40 min, then filtering respectively by silk and filter paper to obtain a filtrate, repeating the above extraction step to process the filter residue for two times by using water or absolute ethanol with the usage amount respectively being 4-8 times and 2-6 times, so as to respectively obtain filtrates, merging the three-time filtrates, performing reduced-pressure concentration and drying, using ultraviolet for sterilization, and then using sterile water to obtain a constant volume and further to obtain the passiflora edulis extractive solution. The passiflora edulis extractive has good bacterial-inhibiting effect on alternaria panax whetzel.
Description
Technical field
The present invention relates to techniques of pest control field, relate in particular to the purposes of Passion flower P.E control pseudo-ginseng black spot.
Background technology
Passionflower (formal name used at school: Passiflora edulis), originates in the torrid zone and the subtropical zone in America.Originate in Brazil, between 1610, import Europe into, Spain missionary found that the pole of figure of its flower portion was like Christ's the cross instrument of torture at that time, 3 divisions on column cap, the utmost point is like 3 nails, petal erythema, stung hemorrhage image just like Jesus's head by rose, 5 flower pesticide, just like injured scar, Spaniard with Passioflos name it, literal translation for " flower of suffering a calamity or disaster ".Also warm meaning of Passion, therefore be also often called as enthusiasm fruit.The flower of passionflower has 5 calyx and 5 petals, as the letter disk on clock, so meter fruit while being called again.The fruit juice of passionflower is often used as spices, is added in other fruit juice.
Pseudo-ginseng (Panax notoginseng F.H.chen) has another name called pseudo-ginseng, invaluable etc.Being the famous and precious special product medicinal material of China, is also one of Yunnan Baiyao primary raw material having won fame both at home and abroad, and it can be enriched blood, go, and addiction is raw new, hemostasis maggot, can lead to and can mend, and effect is good.Sanchi flower, leaf also can be for tea-drinking use, have the refrigerant defecating feces excretion that drives away summer heat, quenches one's thirst, and hypertension Light-headedness is had to good curative effect.Pseudo-ginseng treatment coronary heart diseases and angina pectoris etc. has remarkable result, and physician very payes attention to both at home and abroad.Pseudo-ginseng black spot is pseudo-ginseng one of Major Diseases in recent years, and the producing region masses claim the diseases such as " turning round neck ", " turning round dish ", " turning round lower wall ", " dried flower disease ", " black bar pest ".Its symptom is mostly by due to pseudo-ginseng alternaria (Alternaria panax Whetzel) harm.Each county of mountain of papers Zhuang autonomous prefecture of Yunnan Province in pseudo-ginseng producing region, there is distribution in the counties such as Jingxi, Guangxi Zhuang Autonomous Region, and harm is serious, throughout the year general incidence of disease 20-35%, the serious pseudo-ginseng garden incidence of disease can reach more than 90%.So pseudo-ginseng black spot is to cause the main cause that pseudo-ginseng yield and quality and seed significantly decline in recent years.Passion flower P.E of the present invention has good fungistatic effect to pseudo-ginseng Alternaria alternata caused occurrence (Alternaria panax Whetzel), this bacteriostasis has positive using value, significant for the biological prevention of pseudo-ginseng disease.
Summary of the invention
The object of the invention is to provide a kind of purposes of Passion flower P.E control pseudo-ginseng black spot.
The technical scheme that the present invention takes is:
The purposes of Passion flower P.E control pseudo-ginseng black spot.
The preparation method's of Passion flower P.E of the present invention concrete steps are as follows:
(1) choose anosis, without the fresh body of passionflower of insect pest, clean, dry, then fresh stem is cut into segment, fresh leaf is cut into fragment;
(2) toward the water or the absolute ethyl alcohol that add 5-10 doubly to measure in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 0.5-2h, then ultrasonic 20-40min, filter respectively once with silk and filter paper again, obtain filtrate, filter residue is doubly measured with 4-8 water or the absolute ethyl alcohol doubly measured with 2-6 again and is repeated said extracted step respectively once, obtain respectively filtrate, merge the filtrate of three times, reduced pressure concentration, dry, use ultraviolet sterilization, then obtain Passion flower P.E solution of the present invention with sterile water constant volume.
In step (1), the long 4-5cm of fresh stem segment, the long 2-3cm of fresh leaf fragment, wide 1cm.
In step (2), preferably toward the water or the absolute ethyl alcohol that add 8 times of amounts in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 1h, then ultrasonic 30min.
In step (2), ultrasonic power is 1000W.
In step (2), preferably filter residue repeats said extracted step respectively once with water or the absolute ethyl alcohol of 6 times of amounts and 4 times of amounts again, obtains respectively filtrate.
In step (2), be settled to the Passion flower P.E solution that concentration is 5g/ml with sterile water.
Good effect of the present invention is as follows:
Passion flower P.E of the present invention has good fungistatic effect to pseudo-ginseng Alternaria alternata caused occurrence, experimental result shows: the ethanol extract of passionflower leaf can obviously suppress the growth of pseudo-ginseng alternaric bacteria, in the time that concentration is 1g/ml, inhibiting rate is 40%, and inhibitory action is along with concentration reduces and declines; The water extraction liquid of stem, ethanol extract all can obviously suppress the growth of pseudo-ginseng alternaric bacteria, and in the time that concentration is 1g/ml, inhibiting rate is 30%, and inhibitory action is along with concentration reduces and declines.
Embodiment
The following examples are to describe in further detail of the present invention.
Embodiment 1
1, Passion flower P.E preparation:
Get passionflower anosis, clean, dry without the fresh body of insect pest.Fresh stem is cut into the long segment of 4-5cm, and fresh leaf is cut into long 2-3cm, and wide 1cm fragment is used respectively under 8 times of water gaging room temperatures ultrasonic 0.5h (ultrasonic power is 1000W) after lixiviate 1h, then filters respectively once with silk and filter paper, obtains filtrate; Filter residue repeats above extraction step respectively once with 6 times of amounts and 4 times of water gagings respectively again, obtains respectively filtrate, merges three times filtrate; Reduced pressure concentration, dry, use after ultraviolet sterilization, being settled to concentration with sterile water is 5g/ml solution.
Get passionflower anosis, clean, dry without the fresh body of insect pest.Fresh stem is cut into the long segment of 4-5cm, and fresh leaf is cut into long 2-3cm, and wide 1cm fragment with ultrasonic 0.5h (ultrasonic power is 1000W) after lixiviate 1h under 8 times of amount absolute ethyl alcohol room temperatures, then filters respectively once with silk and filter paper respectively, obtains filtrate; Filter residue repeats above extraction step respectively once with 6 times of amounts and 4 times of amount absolute ethyl alcohols respectively again, obtains respectively filtrate, merges three times filtrate; Reduced pressure concentration, dry, use after ultraviolet sterilization, being settled to concentration with sterile water is 5g/ml solution.
2, the bacteriostasis of passionflower stem, leaf extract
2.1 materials and reagent
The water of passionflower stem, leaf and ethanol extract, PDA medium, analysis water are sterilizing ultra-pure water.For trying bacterial classification: pseudo-ginseng Alternaria alternata caused occurrence chain lattice spore (Alternaria panax Whetzel) is provided by agricultural college of Guangxi University plant pathology laboratory.
Ginseng chain lattice spore (Alternaria panax Whetzel) adopts PDA medium, adopts pathogen growth rate method to measure the inhibition of variable concentrations extract to pathogen.Measure 20ml, 10ml, 5ml passionflower water and ethanol extract for sample, add respectively in the PDA medium of 80ml, 90ml, 95ml sterilizing, make the PDA medium (being respectively 1g/ml, 0.5g/ml and 0.25g/ml for sample solution concentration) of variable concentrations.Pour the PDA medium preparing into culture dish (each culture dish 15ml), after medium is cooling, in the pathogen species of cultivating in advance, beat and get mycelia piece with the card punch (high-temperature sterilization) of diameter 0.5cm, with transfer needle, mycelia piece is transferred to the central authorities of culture dish, one of every culture dish inoculation, be placed at 37 DEG C of biochemical cultivation cases and cultivate, during to the 5th day, take out with right-angled intersection method measurement colony diameter, result is got the mean value that repeats experiment for three times, obtains variable concentrations extract to the fungistatic effect for examination bacterium.
With Williamson propose Sensitivity Index RI as measurement index, RI={ in the time of T >=C, 1-C/T; In the time of T<C, T/C-1 ﹜ C is control value, and T is processing costs.When RI>0, represent facilitation; It when RI<0, is inhibitory action; When RI=0, represent not effect.RI absolute value represents the size of bacteriostasis intensity.
The inhibitory action of table 1. Passion flower P.E to pseudo-ginseng Alternaria alternata caused occurrence
From table 1: the ethanol extract of passionflower leaf can obviously suppress the growth of pseudo-ginseng alternaric bacteria, and in the time that concentration is 1g/ml, inhibiting rate is 40%, inhibitory action is along with concentration reduces and declines; The water extraction liquid of stem, ethanol extract all can obviously suppress the growth of pseudo-ginseng alternaric bacteria, and in the time that concentration is 1g/ml, inhibiting rate is 30%, and inhibitory action is along with concentration reduces and declines.
Although illustrated and described embodiments of the invention, for the ordinary skill in the art, be appreciated that without departing from the principles and spirit of the present invention and can carry out multiple variation, amendment, replacement and modification to these embodiment, scope of the present invention is limited by claims and equivalent thereof.
Claims (7)
1. the purposes of Passion flower P.E control pseudo-ginseng black spot.
2. the preparation method of Passion flower P.E as claimed in claim 1, is characterized in that: the concrete steps of described method are as follows:
(1) choose anosis, without the fresh body of passionflower of insect pest, clean, dry, then fresh stem is cut into segment, fresh leaf is cut into fragment;
(2) toward the water or the absolute ethyl alcohol that add 5-10 doubly to measure in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 0.5-2h, then ultrasonic 20-40min, filter respectively once with silk and filter paper again, obtain filtrate, filter residue is doubly measured with 4-8 water or the absolute ethyl alcohol doubly measured with 2-6 again and is repeated said extracted step respectively once, obtain respectively filtrate, merge the filtrate of three times, reduced pressure concentration, dry, use ultraviolet sterilization, then obtain Passion flower P.E solution of the present invention with sterile water constant volume.
3. preparation method as claimed in claim 2, is characterized in that: in step (1), and the long 4-5cm of fresh stem segment, the long 2-3cm of fresh leaf fragment, wide 1cm.
4. preparation method as claimed in claim 2, is characterized in that: in step (2), toward the water or the absolute ethyl alcohol that add 8 times of amounts in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 1h, then ultrasonic 30min.
5. preparation method as claimed in claim 2, is characterized in that: in step (2), ultrasonic power is 1000W.
6. preparation method as claimed in claim 2, is characterized in that: in step (2), filter residue repeats said extracted step respectively once with water or the absolute ethyl alcohol of 6 times of amounts and 4 times of amounts again, obtains respectively filtrate.
7. preparation method as claimed in claim 2, is characterized in that: in step (2), be settled to the Passion flower P.E solution that concentration is 5g/ml with sterile water.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642398A (en) * | 2015-01-23 | 2015-05-27 | 广西大学 | Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng black spot |
| CN104814056A (en) * | 2015-04-17 | 2015-08-05 | 常州汇森电子有限公司 | Apricot alternaria rot preventing method |
| CN105462849A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot |
| CN105462853A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-59-2 in preventing and controlling panax notoginseng anthracnose |
| CN105462847A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot |
| CN105462851A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-59-2 in preventing and controlling panax notoginseng root rot |
| CN105462852A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng anthracnose |
| CN106342920A (en) * | 2016-08-25 | 2017-01-25 | 文山苗乡三七科技有限公司 | Method for preventing and controlling disease damage and pest damage of pseudo-ginseng by virtue of no- residue farm chemicals |
| CN107951909A (en) * | 2017-12-02 | 2018-04-24 | 桂林理工大学 | A kind of preparation method with flavones in functional passion fruit leaf |
| CN108559632A (en) * | 2018-03-08 | 2018-09-21 | 广西壮族自治区亚热带作物研究所 | A kind of extracting method of passionflower fragrance component |
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2014
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104642398A (en) * | 2015-01-23 | 2015-05-27 | 广西大学 | Application of artemisia verlotorum extract to prevention and control of panax pseudoginseng black spot |
| CN104642398B (en) * | 2015-01-23 | 2017-11-17 | 广西大学 | The purposes of southern biennial wormwood extract preventing and treating Alternaria panax |
| CN104814056A (en) * | 2015-04-17 | 2015-08-05 | 常州汇森电子有限公司 | Apricot alternaria rot preventing method |
| CN104814056B (en) * | 2015-04-17 | 2018-01-19 | 常州汇森电子有限公司 | A kind of method for preventing and treating apricot black spot |
| CN105462847A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng root rot |
| CN105462849B (en) * | 2015-12-23 | 2019-02-05 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-46 in prevention and treatment Alternaria panax |
| CN105462852A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng anthracnose |
| CN105462851B (en) * | 2015-12-23 | 2019-04-02 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-59-2 in prevention and treatment notoginseng root rot |
| CN105462853A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-59-2 in preventing and controlling panax notoginseng anthracnose |
| CN105462849A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-46 in preventing and controlling panax notoginseng black spot |
| CN105462853B (en) * | 2015-12-23 | 2019-04-02 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-59-2 in prevention and treatment Radix Notoginseng anthracnose |
| CN105462851A (en) * | 2015-12-23 | 2016-04-06 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-59-2 in preventing and controlling panax notoginseng root rot |
| CN105462847B (en) * | 2015-12-23 | 2019-01-01 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-46 in prevention and treatment notoginseng root rot |
| CN105462852B (en) * | 2015-12-23 | 2019-01-01 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-46 in prevention and treatment Radix Notoginseng anthracnose |
| CN106342920A (en) * | 2016-08-25 | 2017-01-25 | 文山苗乡三七科技有限公司 | Method for preventing and controlling disease damage and pest damage of pseudo-ginseng by virtue of no- residue farm chemicals |
| CN107951909A (en) * | 2017-12-02 | 2018-04-24 | 桂林理工大学 | A kind of preparation method with flavones in functional passion fruit leaf |
| CN108559632A (en) * | 2018-03-08 | 2018-09-21 | 广西壮族自治区亚热带作物研究所 | A kind of extracting method of passionflower fragrance component |
| CN108559632B (en) * | 2018-03-08 | 2021-12-17 | 广西壮族自治区亚热带作物研究所 | Extraction method of passion flower aroma components |
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