CN104170843A - Application of passion flower extract for prevention and treatment on pseudo-ginseng anthracnose - Google Patents
Application of passion flower extract for prevention and treatment on pseudo-ginseng anthracnose Download PDFInfo
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- CN104170843A CN104170843A CN201410369965.8A CN201410369965A CN104170843A CN 104170843 A CN104170843 A CN 104170843A CN 201410369965 A CN201410369965 A CN 201410369965A CN 104170843 A CN104170843 A CN 104170843A
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Abstract
The invention discloses application of passion flower extract for prevention and treatment on pseudo-ginseng anthracnose, belonging to the technical field of prevention and treatment on plant diseases and insect pests. A preparation method for the passion flower extract comprises the following steps: (1) cleaning and airing selected fresh passion flower bodies without diseases and insect pests, and then cutting fresh stems into small segments, and cutting fresh leaves into chips; and (2) adding 5-10 times amount of water or absolute ethyl alcohol in the fresh stem small segments and the fresh leaf chips of passion flower in the step (1), extracting for 0.5-2 hours at room temperature, then carrying out ultrasonic treatment for 20-40 minutes, then filtering by silk cloth and filter paper respectively to obtain filtrate, repeating the extraction step on the filter residues by 4-8 times amount and 2-6 times amount of water or absolute ethyl alcohol again respectively to obtain filtrates respectively, combining the three times of filtrates, carrying out reduced pressure concentration, drying, sterilizing by an ultraviolet ray, and then making up to the volume by sterile water to obtain the passion flower extract solution disclosed by the invention. The passion flower extract disclosed by the invention is good in antibacterial effect on pseudo-ginseng pathogenic bacteria.
Description
Technical field
The present invention relates to techniques of pest control field, relate in particular to the purposes of Passion flower P.E control pseudo-ginseng anthracnose.
Background technology
Passionflower (formal name used at school: Passiflora edulis), originates in the torrid zone and the subtropical zone in America.Originate in Brazil, between 1610, import Europe into, Spain missionary found that the pole of figure of its flower portion was like Christ's the cross instrument of torture at that time, 3 divisions on column cap, the utmost point is like 3 nails, petal erythema, stung hemorrhage image just like Jesus's head by rose, 5 flower pesticide, just like injured scar, Spaniard with Passioflos name it, literal translation for " flower of suffering a calamity or disaster ".Also warm meaning of Passion, therefore be also often called as enthusiasm fruit.The flower of passionflower has 5 calyx and 5 petals, as the letter disk on clock, so meter fruit while being called again.The fruit juice of passionflower is often used as spices, is added in other fruit juice.
Pseudo-ginseng (Panax notoginsengF.H.chen) has another name called pseudo-ginseng, invaluable etc.Being the famous and precious special product medicinal material of China, is also one of Yunnan Baiyao primary raw material having won fame both at home and abroad, and it can be enriched blood, go, and addiction is raw new, hemostasis maggot, can lead to and can mend, and effect is good.Sanchi flower, leaf also can be for tea-drinking use, have the refrigerant defecating feces excretion that drives away summer heat, quenches one's thirst, and hypertension Light-headedness is had to good curative effect.Pseudo-ginseng treatment coronary heart diseases and angina pectoris etc. has remarkable result.Pseudo-ginseng anthracnose (Colletotriehum panacicofa) is a kind of disease the most generally occurring in Panax notoginseng Growth.The overground part of this disease main harm pseudo-ginseng, leaf portion scab yellowish-brown, has obvious brown edge, on later stage scab raw pore, easily perforation; Petiole and stem mostly are the sagging yellowish-brown prismatic spot of central authorities; The moth patch of rounded nick on fruit, when humidity is larger morbidity serious, one of diseased plant rate the lighter percentage, severe one absolutely.Pseudo-ginseng anthracnose is to cause the one of the main reasons of pseudo-ginseng yield and quality and production declining in recent years.Passion flower P.E of the present invention has good fungistatic effect to pseudo-ginseng anthrax pathogen (Colletotrichum panacicola Vyedaet Takimoto), this bacteriostasis has positive using value, significant for the biological prevention of pseudo-ginseng disease.
Summary of the invention
The object of the invention is to provide a kind of purposes of Passion flower P.E control pseudo-ginseng anthracnose.
The technical scheme that the present invention takes is:
The purposes of Passion flower P.E control pseudo-ginseng anthracnose.
The preparation method's of Passion flower P.E of the present invention concrete steps are as follows:
(1) choose anosis, without the fresh body of passionflower of insect pest, clean, dry, then fresh stem is cut into segment, fresh leaf is cut into fragment;
(2) toward the water or the absolute ethyl alcohol that add 5-10 doubly to measure in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 0.5-2h, then ultrasonic 20-40min, filter respectively once with silk and filter paper again, obtain filtrate, filter residue is doubly measured with 4-8 water or the absolute ethyl alcohol doubly measured with 2-6 again and is repeated said extracted step respectively once, obtain respectively filtrate, merge the filtrate of three times, reduced pressure concentration, dry, use ultraviolet sterilization, then obtain Passion flower P.E solution of the present invention with sterile water constant volume.
In step (1), the long 4-5cm of fresh stem segment, the long 2-3cm of fresh leaf fragment, wide 1cm.
In step (2), preferably toward the water or the absolute ethyl alcohol that add 8 times of amounts in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 1h, then ultrasonic 30min.
In step (2), ultrasonic power is 1000W.
In step (2), preferably filter residue repeats said extracted step respectively once with water or the absolute ethyl alcohol of 6 times of amounts and 4 times of amounts again, obtains respectively filtrate.
In step (2), be settled to the Passion flower P.E solution that concentration is 5g/ml with sterile water.
Good effect of the present invention is as follows:
Passion flower P.E of the present invention has good fungistatic effect to pseudo-ginseng Anthracnose Pathogen bacterium, result shows: the water of passionflower leaf, ethanol extract can obviously suppress pseudo-ginseng anthrax cause of disease bacteria growing, in the time that concentration is 1g/ml, inhibiting rate is respectively 59% and 52%, and along with concentration decline inhibitory action declines not obvious, also show obvious inhibitory action when concentration reaches 0.25g/ml, inhibiting rate is respectively 57% and 46%; Water extraction liquid energy, the ethanol extract of stem all can obviously suppress pseudo-ginseng anthrax cause of disease bacteria growing, in the time that concentration is 1g/ml, inhibiting rate is 55% and 51% respectively, inhibitory action is along with concentration reduces and declines, and in the time that concentration reaches 0.25g/ml, inhibiting rate is respectively 32% and 33%.
Embodiment
The following examples are to describe in further detail of the present invention.
Embodiment 1
1, Passion flower P.E preparation:
Get passionflower anosis, clean, dry without the fresh body of insect pest.Fresh stem is cut into the long segment of 4-5cm, and fresh leaf is cut into long 2-3cm, and wide 1cm fragment is used respectively under 8 times of water gaging room temperatures ultrasonic 0.5h (ultrasonic power is 1000W) after lixiviate 1h, then filters respectively once with silk and filter paper, obtains filtrate; Filter residue repeats above extraction step respectively once with 6 times of amounts and 4 times of water gagings respectively again, obtains respectively filtrate, merges three times filtrate; Reduced pressure concentration, dry, use after ultraviolet sterilization, being settled to concentration with sterile water is 5g/ml solution.
Get passionflower anosis, clean, dry without the fresh body of insect pest.Fresh stem is cut into the long segment of 4-5cm, and fresh leaf is cut into long 2-3cm, and wide 1cm fragment with ultrasonic 0.5h (ultrasonic power is 1000W) after lixiviate 1h under 8 times of amount absolute ethyl alcohol room temperatures, then filters respectively once with silk and filter paper respectively, obtains filtrate; Filter residue repeats above extraction step respectively once with 6 times of amounts and 4 times of amount absolute ethyl alcohols respectively again, obtains respectively filtrate, merges three times filtrate; Reduced pressure concentration, dry, use after ultraviolet sterilization, being settled to concentration with sterile water is 5g/ml solution.
2, the bacteriostasis of passionflower stem, leaf extract
2.1 materials and reagent
The water of passionflower stem, leaf and ethanol extract, PDA medium, analysis water are sterilizing ultra-pure water.For trying bacterial classification: pseudo-ginseng anthrax pathogen (Colletotrichum panacicola Vyedaet Takimoto) is provided by agricultural college of Guangxi University plant pathology laboratory.
Pseudo-ginseng anthrax pathogen (Colletotrichum panacicola Vyedaet Takimoto) adopts PDA medium, adopts pathogen growth rate method to measure the inhibition of variable concentrations extract to pathogen.Measure 20ml, 10ml, 5ml passionflower water and ethanol extract for sample, add respectively in the PDA medium of 80ml, 90ml, 95ml sterilizing, make the PDA medium (being respectively 1g/ml, 0.5g/ml and 0.25g/ml for sample solution concentration) of variable concentrations.Pour the PDA medium preparing into culture dish (each culture dish 15ml), after medium is cooling, in the pathogen species of cultivating in advance, beat and get mycelia piece with the card punch (high-temperature sterilization) of diameter 0.5cm, with transfer needle, mycelia piece is transferred to the central authorities of culture dish, one of every culture dish inoculation, be placed at 37 DEG C of biochemical cultivation cases and cultivate, during to the 5th day, take out with right-angled intersection method measurement colony diameter, result is got the mean value that repeats experiment for three times, obtains variable concentrations extract to the fungistatic effect for examination bacterium.
With Williamson propose Sensitivity Index RI as measurement index, RI={ in the time of T >=C, 1-C/T; In the time of T<C, T/C-1 ﹜ C is control value, and T is processing costs.When RI>0, represent facilitation; It when RI<0, is inhibitory action; When RI=0, represent not effect.RI absolute value represents the size of bacteriostasis intensity.
The inhibitory action of table 1. Passion flower P.E to pseudo-ginseng anthrax pathogen
As can be seen from Table 1: the water of passionflower leaf, ethanol extract can obviously suppress pseudo-ginseng anthrax cause of disease bacteria growing, in the time that concentration is 1g/ml, inhibiting rate is respectively 59% and 52%, and along with concentration decline inhibitory action declines not obvious, also show obvious inhibitory action when concentration reaches 0.25g/ml, inhibiting rate is respectively 57% and 46%; Water extraction liquid energy, the ethanol extract of stem all can obviously suppress pseudo-ginseng anthrax cause of disease bacteria growing, in the time that concentration is 1g/ml, inhibiting rate is 55% and 51% respectively, inhibitory action is along with concentration reduces and declines, and in the time that concentration reaches 0.25g/ml, inhibiting rate is respectively 32% and 33%.
Although illustrated and described embodiments of the invention, for the ordinary skill in the art, be appreciated that without departing from the principles and spirit of the present invention and can carry out multiple variation, amendment, replacement and modification to these embodiment, scope of the present invention is limited by claims and equivalent thereof.
Claims (7)
1. the purposes of Passion flower P.E control pseudo-ginseng anthracnose.
2. the preparation method of Passion flower P.E as claimed in claim 1, is characterized in that: the concrete steps of described method are as follows:
(1) choose anosis, without the fresh body of passionflower of insect pest, clean, dry, then fresh stem is cut into segment, fresh leaf is cut into fragment;
(2) toward the water or the absolute ethyl alcohol that add 5-10 doubly to measure in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 0.5-2h, then ultrasonic 20-40min, filter respectively once with silk and filter paper again, obtain filtrate, filter residue is doubly measured with 4-8 water or the absolute ethyl alcohol doubly measured with 2-6 again and is repeated said extracted step respectively once, obtain respectively filtrate, merge the filtrate of three times, reduced pressure concentration, dry, use ultraviolet sterilization, then obtain Passion flower P.E solution of the present invention with sterile water constant volume.
3. preparation method as claimed in claim 2, is characterized in that: in step (1), and the long 4-5cm of fresh stem segment, the long 2-3cm of fresh leaf fragment, wide 1cm.
4. preparation method as claimed in claim 2, is characterized in that: in step (2), toward the water or the absolute ethyl alcohol that add 8 times of amounts in the fresh stem segment of passionflower of step (1) and fresh leaf fragment, at room temperature lixiviate 1h, then ultrasonic 30min.
5. preparation method as claimed in claim 2, is characterized in that: in step (2), ultrasonic power is 1000W.
6. preparation method as claimed in claim 2, is characterized in that: in step (2), filter residue repeats said extracted step respectively once with water or the absolute ethyl alcohol of 6 times of amounts and 4 times of amounts again, obtains respectively filtrate.
7. preparation method as claimed in claim 2, is characterized in that: in step (2), be settled to the Passion flower P.E solution that concentration is 5g/ml with sterile water.
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| CN201410369965.8A CN104170843A (en) | 2014-07-30 | 2014-07-30 | Application of passion flower extract for prevention and treatment on pseudo-ginseng anthracnose |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483021A (en) * | 2015-12-23 | 2016-04-13 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng anthrax |
| CN108976309A (en) * | 2018-06-29 | 2018-12-11 | 桂林理工大学 | Polysaccharide extracting process and optimization of orthogonal test in passionflower leaf |
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| JPH08193032A (en) * | 1994-07-14 | 1996-07-30 | Emil Flachsmann Ag | Preparation of concentrate of active ingredient of plant that has few agricultural chemical content |
| CN103609621A (en) * | 2013-10-25 | 2014-03-05 | 青岛华仁技术孵化器有限公司 | Traditional Chinese medicine insecticidal composition |
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2014
- 2014-07-30 CN CN201410369965.8A patent/CN104170843A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH08193032A (en) * | 1994-07-14 | 1996-07-30 | Emil Flachsmann Ag | Preparation of concentrate of active ingredient of plant that has few agricultural chemical content |
| CN103609621A (en) * | 2013-10-25 | 2014-03-05 | 青岛华仁技术孵化器有限公司 | Traditional Chinese medicine insecticidal composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483021A (en) * | 2015-12-23 | 2016-04-13 | 广西大学 | Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng anthrax |
| CN105483021B (en) * | 2015-12-23 | 2019-04-05 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-34-1 in prevention and treatment Radix Notoginseng anthracnose |
| CN108976309A (en) * | 2018-06-29 | 2018-12-11 | 桂林理工大学 | Polysaccharide extracting process and optimization of orthogonal test in passionflower leaf |
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