CN104642400B - The purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose - Google Patents
The purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose Download PDFInfo
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- CN104642400B CN104642400B CN201510035946.6A CN201510035946A CN104642400B CN 104642400 B CN104642400 B CN 104642400B CN 201510035946 A CN201510035946 A CN 201510035946A CN 104642400 B CN104642400 B CN 104642400B
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Abstract
The invention discloses the purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose, belong to plant pest management technical field, the preparation method of the southern biennial wormwood extract is as follows：(1) the clean rear naturally dry of disease-free southern argy wormwood stem, the fresh body of leaf is chosen, fresh body grinding and sieving, then stem, leaf powder is mixed and is placed in drying receptacle；(2) toward addition ethanol progress first time filtering in southern argy wormwood powder obtained by step (1), filtrate is obtained, gained filter residue is added into ethanol filter for the second time, after repetition is filtered 2 times, merges 3 filtrates；(3) it is then to obtain the southern biennial wormwood extract of the present invention with sterilized water constant volume using ultraviolet sterilization by filtrate obtained by step (2) through being concentrated under reduced pressure.The southern biennial wormwood extract of the present invention has good fungistatic effect to pseudo-ginseng Anthracnose Pathogen bacterium.
Description
Technical field
The present invention relates to techniques of pest control field, more particularly to the use of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose
On the way.
Background technology
Southern argy wormwood (Artemisia verlotorum Lamotte), belongs to composite family artemisia, nickname Artemisia santonica, Artemisia stelleriana.Due to
Sagebruss is similar on profile, smell, also may be collectively referred to herein as " wormwood " or " argy wormwood ", big portion of China, and Europe, Asia and Africa etc. is all
It is distributed.This plant is adopted to be perennial, plant has fragrance.Main root is slightly obvious, and lateral root is more.Stem list is raw or a small number of, high 50-
100cm, the long 5-8cm of branch, is affixed to stem obliquely.It is seen everywhere, is occupied an important position in traditional Chinese culture in the wild.
It can be used as medicine, there is anti-inflammatory, anastalsis.
Pseudo-ginseng (Panax notoginseng F.H.chen) also known as pseudo-ginseng, invaluable etc., have it is significant it is promoting blood circulation and removing blood stasis,
Detumescence ding-tong effect, it is a kind of Chinese tradition rare medicinal herbs.Using pseudo-ginseng as Chinese patent drug made of primary raw material such as " Yunnan Baiyao "
" Pien Tze Huang " etc. is wide-spread.In recent years, it is growing to the demand of pseudo-ginseng raw material.But the disease in cultivation is tight
Panax notoginseng Growth is have impact on again, and the abuse of chemical pesticide also have impact on the quality of pseudo-ginseng.Pseudo-ginseng anthracnose is that Panax notoginseng Growth is common
One of disease, seedling stage, Adult plant can fall ill.Blade early stage shows as blade life yellowish-brown spot, and later stage disease portion easily perforates
Rupture.Yellowish-brown depression spot can be formed after petiole and stem infection.Become brown after fruit infection to rot.
The southern biennial wormwood extract of the present invention is to pseudo-ginseng anthrax pathogen (Colletotrichum panacicola
Vyedaet Takimoto) there is good fungistatic effect, this bacteriostasis has positive application value, to pseudo-ginseng anthrax
The biological control of disease is significant.
The content of the invention
Present invention aims at provide a kind of purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose.It is provided by the present invention
Technical scheme is：
The invention provides the purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose.
In addition, present invention also offers the preparation method of southern biennial wormwood extract, it is comprised the following steps that：
(1) disease-free southern argy wormwood stem is chosen, the fresh body of leaf cleans rear naturally dry, fresh body grinding and sieving, then by stem, leaf
Powder mixing is placed in drying receptacle；
(2) first time filtering is carried out toward addition ethanol in southern argy wormwood powder obtained by step (1), obtains filtrate, gained is filtered
Slag adds ethanol and carries out second of filtering, repeats after filtering 2 times, merges 3 filtrates；
(3) by filtrate obtained by step (2) through being concentrated under reduced pressure, using ultraviolet sterilization, then obtained with sterilized water constant volume
The southern biennial wormwood extract of the present invention.
In technical solution of the present invention, sieving described in step (1) uses the aperture of sieve as 50 mesh.
In technical solution of the present invention, step filters for the first time described in (2), is that 8 times of amount second are added in southern argy wormwood powder
Alcohol soaks 4d at room temperature, then with four layers of filtered through gauze, obtains filtrate.
Second of filtering described in step (2), it is that will filter gained filter residue first time with 6 times in technical solution of the present invention
Amount ethanol soaks 4d at room temperature, then with four layers of filtered through gauze, obtains filtrate.
In technical solution of the present invention, the concentration of step (3) Central-South biennial wormwood extract is 2.0gmL-1。
Compared with prior art, the device have the advantages that being：
The southern biennial wormwood extract of the present invention can substantially suppress pseudo-ginseng anthrax pathogen, and its inhibitory action is with crude drug concentration
Reduction, incubation time extension and reduce.
Embodiment
With reference to specific embodiment, make further details of elaboration to the present invention, but embodiments of the present invention are not
It is confined to the scope of embodiment expression.
Embodiment 1：The preparation of southern biennial wormwood extract
Choose disease-free southern argy wormwood stem, the fresh body of leaf cleans rear naturally dry.Fresh body crushed the sieve that aperture is 50 mesh, so
Its stem, leaf powder are mixed and be placed in drying receptacle afterwards, 8 times of amount ethanol is added and soaks 4d at room temperature, then with four layers of gauze mistake
Filter, obtains filtrate.Filter residue repeats previous step dip operation with 6 times of amount ethanol, after being repeated 2 times, merges filtrate three times.Filtrate subtracts
Pressure, concentration, after ultraviolet sterilization, it is 2.0gmL to be then settled to crude drug concentration with sterilized water-1。
Embodiment 2：The bacteriostasis of southern biennial wormwood extract
2.1 materials and reagent
Southern argy wormwood ethanol extract, sterilized water, PDA culture medium.For trying strain：Pseudo-ginseng anthrax pathogen
(Colletotrichum panacicola Vyedaet Takimoto) is carried by agricultural college of Guangxi University plant pathology laboratory
For.
2.2 method
The fungistatic effect of southern biennial wormwood extract is determined using mycelial growth rate method.It is 2.0gmL to measure crude drug concentration-1
Southern argy wormwood leaching liquor 6.75ml, 3.375ml, 1.6875ml, be settled to 10ml with sterilized water respectively, be made various concentrations south
Argy wormwood test solution, takes the southern argy wormwood test liquid 1ml of various concentrations respectively, add with the PDA culture medium 14ml melted in
Drug containing flat board is made in 90mm culture dishes, it is finally 0.0900gmL for examination concentration to make it-1、0.0450g·mL-1With
0.0225g·mL-1, to add equivalent sterilized water as control.After culture medium solidifying, use and beat in the pathogen species cultivated in advance
Hole device, which is beaten, takes diameter 6mm mycelia blocks, is transferred to the center of culture dish, one piece, 28 DEG C of culture 96h is inoculated with per culture dish, each confession
Try concentration in triplicate.Colony diameter is measured using crossing method in the 48th, 72h, 96h, takes the average result repeated three times,
Various concentrations extract is obtained to the inhibition for thing bacterium.Following formula calculate the inhibiting rate to pathogen：
Bacteriostasis rate (%)=[(the pure increment of the control bacterium colony-pure increment of processing bacterium colony)/pure increment of control bacterium colony] ×
100%；Pure increment=bacterium colony average diameter-bacteria cake diameter
Inhibitory action of the southern biennial wormwood extract of the various concentrations of table 1 to pseudo-ginseng Anthracnose Pathogen bacterium
As shown in Table 1, it is 0.0900gmL in southern biennial wormwood extract crude drug concentration-1Inhibiting rate is up to when cultivating 48h
It can also reach 74.83% after 89.66%, 96h, it is seen then that southern biennial wormwood extract can substantially suppress the life of pseudo-ginseng anthrax pathogen
It is long；Its inhibitory action reduces with the reduction of crude drug concentration.
The description of the foregoing specific illustrative embodiment to the present invention is to illustrate and the purpose of illustration.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (5)
1. the purposes of southern biennial wormwood extract preventing and treating pseudo-ginseng anthracnose, it is characterised in that the preparation method of the southern biennial wormwood extract,
Comprise the following steps that：
(1) disease-free southern argy wormwood stem is chosen, the fresh body of leaf cleans rear naturally dry, fresh body grinding and sieving, then by stem, leaf powder
Mixing is placed in drying receptacle；
(2) first time filtering is carried out toward addition ethanol in southern argy wormwood powder obtained by step (1), obtains filtrate, by gained filter residue again
Addition ethanol carries out second and filtered, and repeats after filtering 2 times, merges 3 filtrates；
(3) by filtrate obtained by step (2) through being concentrated under reduced pressure, using ultraviolet sterilization, then this hair is obtained with sterilized water constant volume
Bright southern biennial wormwood extract.
2. purposes as claimed in claim 1, it is characterised in that：Sieving described in step (1) uses the aperture of sieve as 50 mesh.
3. purposes as claimed in claim 1, it is characterised in that：Step filters for the first time described in (2), is in southern argy wormwood powder
It is middle to add 8 times of amount ethanol and soak 4d at room temperature, then with four layers of filtered through gauze, obtain filtrate.
4. purposes as claimed in claim 1, it is characterised in that：Second of filtering described in step (2), it is to filter first time
Gained filter residue soaks 4d at room temperature with 6 times of amount ethanol, then with four layers of filtered through gauze, obtains filtrate.
5. purposes as claimed in claim 1, it is characterised in that：The concentration of step (3) Central-South biennial wormwood extract is 2.0gmL-1。
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| CN105494334B (en) * | 2015-12-08 | 2018-03-13 | 北京农学院 | A kind of environmentally friendly bactericide for preventing and treating fruit tree bacterial disease and preparation method thereof |
| CN105483021B (en) * | 2015-12-23 | 2019-04-05 | 广西大学 | Application of the sophora tonkinensis Gapnep endogenetic fungus TRXY-34-1 in prevention and treatment Radix Notoginseng anthracnose |
| CN108850145B (en) * | 2018-06-11 | 2021-08-20 | 南阳道地金木瓜生物科技股份有限公司 | Papaya storage and preservation method |
Citations (3)
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|---|---|---|---|---|
| CN103329945A (en) * | 2013-05-31 | 2013-10-02 | 句容市杨修林果园 | Natural bactericide for controlling peach tree anthracnose and preparation method |
| CN103404541A (en) * | 2013-06-28 | 2013-11-27 | 句容市白兔镇沁香果品专业合作社 | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide |
| CN103597982A (en) * | 2013-10-29 | 2014-02-26 | 青岛东颐锦禾农业科技有限公司 | Use method of compound drug for treating anthracnose |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103329945A (en) * | 2013-05-31 | 2013-10-02 | 句容市杨修林果园 | Natural bactericide for controlling peach tree anthracnose and preparation method |
| CN103404541A (en) * | 2013-06-28 | 2013-11-27 | 句容市白兔镇沁香果品专业合作社 | Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide |
| CN103597982A (en) * | 2013-10-29 | 2014-02-26 | 青岛东颐锦禾农业科技有限公司 | Use method of compound drug for treating anthracnose |
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| 艾蒿提取物对13种植物病原菌物的抑制作用;江茂生等;《福建农林大学学报(自然科学版)》;20070731;第36卷(第4期);第352-356页；第1.1.1和1.1.3节，表1以及表2 * |
| 蒿属植物的农药活性及其有效成分;周利娟等;《江西农业大学学报》;20120830;第34卷(第4期);第699-705页；第2节 * |
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