CN106566777B - Trichoderma pseudokiningii T5-1 bacterial strain and its application in promotion Panax notoginseng Growth and root rot prevention and control - Google Patents
Trichoderma pseudokiningii T5-1 bacterial strain and its application in promotion Panax notoginseng Growth and root rot prevention and control Download PDFInfo
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Abstract
The invention belongs to plant disease Control Technology fields, and in particular to trichoderma pseudokiningii T5-1 bacterial strain and its application in promotion Panax notoginseng Growth and root rot prevention and control.The strain classification is named as trichoderma pseudokiningii (Trichoderma koningiopsis), is CGMCC NO.12779 in the deposit number of China Microbial Culture Preservation Commission's common micro-organisms center.Trichoderma pseudokiningii T5-1 bacterial strain mycelia is flourishing, and aerial hyphae is fluffy dense, and non-pigment generates.Trichoderma pseudokiningii T5-1 bacterial strain can promote Panax notoginseng Growth, simultaneously, bacterial strain can inhibit pine root fungus growth, the heavy responsibilities of government are born in pine root fungus, the volatile organic compounds of bacterial strain inhibits the production spore of pine root fungus in confined conditions, for the exploitation of bio-bacterial manure and its using establishing good basis.
Description
Technical field
The invention belongs to plant disease Control Technology fields, and in particular to trichoderma pseudokiningii T5-1 bacterial strain and its promote three
Application in seven growths and root rot prevention and control.
Background technique
The prevention and control of plant disease, the most quickly and effectively means are using chemical bactericide.But it is long-term a large amount of using chemical
Fungicide will cause the generation of environmental pollution, ecosystem destruction, pathogenic bacteria resistance to drugs and drug resistance, and in addition pesticide residue is asked
Topic also seriously restricts crop safety and produces and threaten human health.Current chemical pesticide is still occupied an leading position, but because biocontrol microorganisms have
Have the advantages that using it is safe, do not develop drug resistance, as a kind of green, environmental-friendly preventions, especially to soil
Biocontrol microorganisms are added in earth and carry out prevention and control soil-borne disease, are had been to be concerned by more and more people, and be increasingly becoming the following ecological agriculture to hold
The important directions of supervention exhibition.Meanwhile microbial-bacterial fertilizer use can also alleviate because of a large amount of uses of chemical fertilizer caused by soil
The problems such as hardened, soil physico-chemical property changes.The use premise of microbial-bacterial fertilizer is to obtain to have the function of efficient bio-active
Beneficial microbe.Biocontrol microorganisms by with pathogen competitive space, generate antibacterial substance, hyperparasite pathogen, induction plant
The direct or indirect prevention and control plant disease of system resistant and promotion plant growth etc..With the development of molecular biology, raw
Object technology is to the maturation of microorganism renovation technique, and biocontrol microorganisms are in agricultural production as a kind of substitution pesticide or at least can conduct
The status for reducing a kind of means of supplementing out economy of chemical pesticide usage amount is more and more secured, while showing huge economic benefit, society
Meeting benefit and ecological benefits also comply with the environmentally protective target with sustainable development of the mankind.
Radix Notoginseng and ginseng, American Ginseng etc. are equal to be belonged to, and the good reputations such as " southern part of the country god grass ", " invaluable " are enjoyed, and is that China is peculiar
Rare traditional Chinese medicine has hemostasis, activating microcirculation and removing stasis medicinal, reducing blood lipid, antithrombotic and other effects, especially has special effect to traumatic injury etc..Radix Notoginseng
Demand to ecological environment is more harsh, ecological amplitude is relatively narrow, growth cycle is longer, with the increase of Planting Years, disease in soil
Opportunistic pathogen constantly accumulates, and disease incidence is also continuously increased, and is caused damages throughout the year up to 10%-40%, serious to reach 70% or more,
The yield and quality for seriously affecting Radix Notoginseng becomes and restricts the major obstacle that Radix Notoginseng industry develops in a healthy way.Radix Notoginseng is mainly planted at present
It is saved in Yunnan Province of China, cultivated area is more than 300,000 mu, and worldwide occupation rate of market is up to 85% or more.With people
Promotion of the raising and society of health perception to cardiovascular diseases drug demand, the market demand of Radix Notoginseng are continuously increased.Thus
Bring single area plantation, abuse of chemical pesticide etc. on a large scale, so that Radix Notoginseng Pesticide Residue is more prominent.Therefore,
Using biological preventions prevention and control notoginseng root rot, have to the sustainable development of the quality and healthful Chinese crud drug that promote Radix Notoginseng product
There is important realistic meaning.
Notoginseng root rot fungal pathogens reported at present mainly include Fusarium (Fusarium spp.), column spore Pseudomonas
(Cylindrocarpon spp.), Phytophthora (Phytophthora spp.), Phoma (Phoma spp.), Rhizoctonia
(Rhizoctonia spp.) and quasi- Pestalotia (Pestalotiopsis spp.) etc. 6 categories.
Summary of the invention
The purpose of the present invention is to propose to trichoderma pseudokiningii T5-1 bacterial strain and its promoting Panax notoginseng Growth and root rot prevention and control
In application.Specific technical solution is as follows:
Trichoderma pseudokiningii T5-1 bacterial strain, classification naming are trichoderma pseudokiningii (Trichoderma koningiopsis),
The deposit number of China Microbial Culture Preservation Commission's common micro-organisms center is CGMCC NO.12779.
The trichoderma pseudokiningii T5-1 bacterial strain cultivates 3d on glucose potato agar culture medium PDA, can cover with diameter
For the culture dish of 9cm, mycelia is flourishing, and aerial hyphae is fluffy dense, and non-pigment generates；The PDA culture medium composition are as follows: potato
200g/L, glucose 20g/L, agar 20g/L.
The trichoderma pseudokiningii T5-1 bacterial strain is promoting the application in Panax notoginseng Growth and root rot prevention and control.
The trichoderma pseudokiningii T5-1 bacterial strain is promoting the application in Panax notoginseng Growth, by using trichoderma pseudokiningii bacterium T5-1
Bacterial strain conidial suspension carries out root irrigation to Radix Notoginseng, promotes Panax notoginseng Growth.
The concentration of the trichoderma pseudokiningii bacterium T5-1 bacterial strain conidial suspension is 1 × 104~1 × 107A mitogenetic spore
Son/mL.
The preparation method of the trichoderma pseudokiningii bacterium T5-1 bacterial strain conidial suspension, comprising the following steps:
1) by trichoderma pseudokiningii T5-1 strain inoculated in cultivating in PDA culture medium, trichoderma pseudokiningii T5-1 bacterial strain bacterium is obtained
It falls；
2) in the culture dish of step 1) be added 20mL sterile water, scrape the conidium on surface, with 4 layers of sterile gauze into
Row filters to arrive trichoderma pseudokiningii T5-1 conidial suspension.
Condition of culture is 28 DEG C of dark culturing 6d in step 1).
Application of the trichoderma pseudokiningii T5-1 bacterial strain in notoginseng root rot prevention and control, the pathogen of the root rot are to destroy column
Spore bacterium (C.destructans), Phytophthora cactorum bacterium (P.cactorum), one kind of sickle-like bacteria (F.solani) or more.
The trichoderma pseudokiningii T5-1 bacterial strain filtrate inhibits pine root fungus growth.
The preparation of the trichoderma pseudokiningii T5-1 bacterial strain filtrate includes the following steps:
1) by trichoderma pseudokiningii T5-1 strain inoculated in cultivating in PDA culture medium, trichoderma pseudokiningii T5-1 bacterial strain bacterium is obtained
It falls；
2) the trichoderma pseudokiningii T5-1 colony edge obtained from step 1) beats 20 pieces of mycelia block for taking 5mm, is seeded to and contains
It in the 500mL triangular flask of 300mL PDB culture solution, after shaken cultivation 30d, is filtered using vacuum pump, obtains trichoderma pseudokiningii T5-
1 bacterial strain filtrate；The PDB culture medium composition are as follows: potato 200g/L, glucose 20g/L.
Condition of culture is 28 DEG C of dark culturing 2d in step 1)；Condition of culture is 25 DEG C, 180rpm/min in step 2).
The volatile organic compounds of the trichoderma pseudokiningii T5-1 bacterial strain inhibits the production of pine root fungus in confined conditions
Spore.
The trichoderma pseudokiningii T5-1 bacterial strain hyperparasite pine root fungus.
The invention has the benefit that the trichoderma pseudokiningii T5-1 bacterial strain mycelia of screening is flourishing, aerial hyphae is fluffy dense,
Non-pigment generates.Trichoderma pseudokiningii T5-1 bacterial strain can promote Panax notoginseng Growth, meanwhile, bacterial strain can inhibit pine root fungus and grow, again
Parasitize pine root fungus, the volatile organic compounds of bacterial strain inhibits the production spore of pine root fungus in confined conditions, reach promotion
Panax notoginseng Growth and the double effects for inhibiting root rot establish good basis for the exploitation and its utilization of bio-bacterial manure.
Biomaterial preservation explanation
Classification naming: trichoderma pseudokiningii (Trichoderma koningiopsis) strain number: T5-1
Preservation mechanism: China General Microbiological culture presevation administrative center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on 08 08th, 2016
Collection is registered on the books number: CGMCC No.12779
Detailed description of the invention
Fig. 1 is morphological feature of the trichoderma pseudokiningii T5-1 bacterial strain in PDA culture medium, and wherein a is colonial morphology, and b is
Conidiophore form, c are conidium form.
Fig. 2 is trichoderma pseudokiningii T5-1 bacterial strain filtrate to the inhibitory effect of Phytophthora cactorum bacterium D-10, and wherein a is unused T5-1 bacterium
The colonial morphology of the Phytophthora cactorum bacterium D-10 of strain filtrate processing, b are the inhibition after T5-1 bacterial strain filtrate is handled to Phytophthora cactorum bacterium D-10
Effect.
Fig. 3 is trichoderma pseudokiningii T5-1 bacterial strain filtrate to the inhibitory effect of Phytophthora cactorum bacterium D-10, and wherein CK is unused T5-1
The processing of bacterial strain filtrate, T5-1-Ster-D-10 is for T5-1 bacterial strain filtrate to Phytophthora cactorum bacterium D- after 121 DEG C of high-temperature process 20min
10 inhibiting rate.
Fig. 4 is the inhibitory effect that trichoderma pseudokiningii T5-1 bacterial strain volatile organic compounds produces spore to sickle-like bacteria F-2, wherein
F-2 is the Sporulation of Fusarium situation of unused T5-1 volatile materials processing, and T5-1-F-2 is with T5-1 TREATMENT OF VOCs
Sporulation of Fusarium situation afterwards, A and B indicate processing group and control group, and there are extremely significant differences in 1% level.
Fig. 5 is the effect of trichoderma pseudokiningii T5-1 hyperparasite notoginseng root rot bacterium, and wherein a is the weight in a manner of spore adherency
Parasitic Cylindrocarpon destructans, b are the hyperparasite Cylindrocarpon in a manner of mycelia winding
Destructans, c are the hyperparasite Phtophthora cactorum in a manner of formation " hook-shaped " structure.
Specific embodiment
The invention proposes trichoderma pseudokiningii T5-1 bacterial strain and its promoting the application in Panax notoginseng Growth and root rot prevention and control,
Below with reference to embodiment, the present invention will be further described.
Embodiment 1: the separation and identification of trichoderma pseudokiningii T5-1 bacterial strain
1, the isolation and purification of bacterial strain
From Yunnan Province, Wenshan Prefecture obtains 3 years raw sangqi ginseng root tissue samples, with tap water by the soil of Roots of Panax Notoginseng tissue surface
Etc. rinsing well；It cuts Radix Notoginseng root tissue disease and is good for intersection tissue block, size about 4mm2；Time by the tissue block cut 1%
It sterilizes 3min in sodium chlorate solution, after aseptic water washing 3 times, is sufficiently dried in sterilizing filter paper on piece；Tissue block is placed in and is added
On PDA plate added with streptomycin sulphate (100ppm), culture 1-3d, routine observation are inverted in 28 DEG C of constant incubators；To
After growing doubtful bacterium colony in culture dish, carry out picking immediately and be transferred on fresh PDA plate that preservation is numbered；Using bacterium
Silk end method is purified, and pure culture as shown in Figure 1 is obtained.The PDA plate composition are as follows: potato 200g/L, grape
Sugared 20g/L, agar 20g/L.
2, the identification of bacterial strain
The genomic DNA of bacterial strain after extraction separation and purification carries out PCR amplification using following 3 pairs of primers:
1. upstream primer ITS1 (5 '-TCCGTAGGTGAACCTGCGG-3 ') and downstream primer ITS2 (5 '-
GCTGCGTTCTTCATCGATGC-3 '), extension increasing sequence is as shown in SEQ ID No.1.
2. upstream primer ITS1 (5 '-TCCGTAGGTGAACCTGCGG-3 ') and downstream primer ITS4 (5 '-
CCTCCGCTTATTGATATGC-3 '), extension increasing sequence is as shown in SEQ ID No.2.
3. upstream primer tef1 (5 '-GTGAGCGTGGTATCACCATCG-3 ') and downstream primer tef1 (5 '-
GCCATCCTTGGAGACCAGC-3 '), extension increasing sequence is as shown in SEQ ID No.3.
By above-mentioned extension increasing sequence Trichoderma database TrichoBLAST (http://www.isth.info/tools/ blast/preblast.php) in be compared, classification naming be trichoderma pseudokiningii (Trichoderma koningiopsis),
Strain Designation is T5-1.
Embodiment 2: growth-promoting functions of the trichoderma pseudokiningii T5-1 bacterial strain conidial suspension to Radix Notoginseng
By trichoderma pseudokiningii T5-1 strain inoculated in 28 DEG C of dark culturing 6d in PDA culture medium, trichoderma pseudokiningii T5- is obtained
1 bacterial strain bacterium colony；20mL sterile water is added in culture dish, scrapes the conidium on surface, is filtered with 4 layers of sterile gauze,
Trichoderma pseudokiningii T5-1 conidial suspension is obtained, concentration is 1 × 107A conidium/mL.
The annual Radix Notoginseng plant in field and biennial Radix Notoginseng plant are provided by Yunnan Province Miao Xiang Radix Notoginseng Technology Park.Using with
The experimental design of machine district's groups, plot area/Radix Notoginseng strain number of setting are as follows: about 100 plants of annual Radix Notoginseng 0.5m × 0.5m/；2 years
About 30 plants of raw sangqi ginseng 1.5m × 0.5m/；The spore suspension concentration of the trichoderma pseudokiningii T5-1 bacterial strain of 3 gradient dilutions is set
(A dilutes 10 times；B dilutes 100 times；C dilutes 1000 times) regular root irrigation is carried out, 3 repetitions are arranged in each concentration, each
Cell pouring root amount is 500mL, carries out a root irrigation every 7d, carries out 4 pouring roots altogether, control group is substituted with tap water.
According to five point sampling, when annual Radix Notoginseng 20d after treatment, biennial Radix Notoginseng 45d, is sampled.1 year
The each cell of raw sangqi ginseng samples 10 plants (totally 30 plants of 3 repetitions)；The each cell of biennial Radix Notoginseng samples 5 plants of (3 repetitions totally 15
Strain).The measurement that middle period long, middle leaf width, plant height, overground part fresh weight, underground part fresh weight is carried out to the single plant of samples taken, to each
All samples in repeating carry out the measurement of overground part dry weight, underground part dry weight total weight.By the above measurement index, use
DPS software carries out significant difference analysis.As a result such as Tables 1 and 2, the results show that trichoderma pseudokiningii T5-1 bacterial strain has Radix Notoginseng
Growth-promoting functions.
Growth-promoting effect of the 1 trichoderma pseudokiningii T5-1 bacterial strain of table to annual Radix Notoginseng
Note: infusing * after data, illustrates variant conspicuousness (P < 0.05) compared with the control.
Growth-promoting effect of the 2 trichoderma pseudokiningii T5-1 bacterial strain of table to biennial Radix Notoginseng
Note: infusing * after data, illustrates variant conspicuousness (P < 0.05) compared with the control.
Embodiment 3: inhibiting effect of the trichoderma pseudokiningii T5-1 bacterial strain filtrate to Phytophthora cactorum.
By trichoderma pseudokiningii T5-1 strain inoculated in 28 DEG C of dark culturing 2d in PDA culture medium, trichoderma pseudokiningii T5- is obtained
1 bacterial strain bacterium colony；20 pieces of mycelia block for taking 5mm are beaten, are seeded in the 500mL triangular flask of the culture solution of PDB containing 300mL, 25 DEG C,
It under the conditions of 180rpm/min after shaken cultivation 30d, is filtered using vacuum pump, obtains trichoderma pseudokiningii T5-1 bacterial strain filtrate；Filtrate
In 121 DEG C of heat treatment 20min, and it is sufficiently mixed with PDA culture medium according to volume ratio 1:4, inverted plate, every plate about 20mL；With
The punch of 5mm takes the Phytophthora cactorum colony edge mycelia block of activation 4d, 9cm plate center is placed in, after cultivating 10d, using ten
Word interior extrapolation method measures the colony diameter of Phytophthora cactorum bacterium, calculates inhibiting rate.As a result such as Fig. 2 and Fig. 3, the filter of trichoderma pseudokiningii T5-1 bacterial strain
Liquid is obvious to the inhibitory effect of Phytophthora cactorum bacterium.
Embodiment 4: inhibiting effect of the trichoderma pseudokiningii T5-1 bacterial strain volatile organic compounds to Sporulation of Fusarium
Using the method for make-up culture.Trichoderma pseudokiningii T5-1 bacterial strain is activated respectively with sickle-like bacteria, 3 days to be grown
Afterwards, it is beaten respectively in the colony edge of trichoderma pseudokiningii T5-1 bacterial strain and sickle-like bacteria with 5mm punch and takes fresh mycelia block；By quasi- health
Family name's trichoderma T5-1 bacterial strain mycelia block is placed on the 9cm culture dish center containing 20mLPDA, and it is new that sickle-like bacteria mycelia block is placed on another
The fresh 9cm culture dish center containing 20mLPDA；By two mutual make-ups in ware bottom, 4 layers of package processing are carried out with sealed membrane, each
Handle 3 repetitions；The one side of the block of mycelia containing sickle-like bacteria upper, the one side of the bacterial strain mycelia block of T5-1 containing trichoderma pseudokiningii under, with
Two sides all connects the processing of sickle-like bacteria as blank control, carries out culture 7d in 28 DEG C of constant incubators；Beaten with 5mm punch take by
Trichoderma pseudokiningii T5-1 bacterial strain volatile organic compounds treated sickle-like bacteria mycelia block is simultaneously transferred to fresh PDA culture medium
After upper progress restoration ecosystem 5d, near vaccination, vaccination and 3 midpoint of colony edge line, colony edge positions, use
5mm punch, which is beaten, takes mycelia block, is placed in the PCR pipe of the aqua sterilisa containing 1mL, and the statistics of sporulation quantity is carried out after shaking up.As a result as schemed
4, trichoderma pseudokiningii T5-1 bacterial strain volatile organic compounds produces spore to sickle-like bacteria F-2 and generates inhibitory effect.
Embodiment 5: the effect of trichoderma pseudokiningii T5-1 hyperparasite notoginseng root rot bacterium
Using the method for opposite culture.By trichoderma pseudokiningii T5-1, destroy column spore bacterium (C.destructans) and Phytophthora cactorum
Bacterium (P.cactorum) is activated, and after 3 days to be grown, is beaten respectively with 5mm punch and is taken mycelia block；By trichoderma pseudokiningii T5-1
Mycelia block is placed on the 9cm culture dish side (away from edge about 2cm or so) of the PDA containing 20mL, destroys column spore bacterium and Phytophthora cactorum bacterium
Mycelia block is placed at the culture dish other side (away from edge about 2cm or so), and culture is inverted in 28 DEG C of constant incubators.It is cultivating respectively
When 4d and 8d, mycelia intersection is cut with sterilizing blade, size about 4mm × 4mm is scanned electron microscope observation.
As a result such as Fig. 5, trichoderma pseudokiningii T5-1 can the hyperparasite Cylindrocarpon destructans in such a way that spore adheres to；With
The mode hyperparasite Cylindrocarpon destructans of mycelia winding；The hyperparasite in a manner of formation " hook-shaped " structure
Phtophthora cactorum。
SEQUENCE LISTING
<110>China Agricultural University
<120>trichoderma pseudokiningii T5-1 bacterial strain and its application in promotion Panax notoginseng Growth and root rot prevention and control
<130> 2016
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<170> PatentIn version 3.3
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Claims (13)
1. trichoderma pseudokiningii T5-1 bacterial strain, which is characterized in that the strain classification is named as trichoderma pseudokiningii (Trichoderma
It koningiopsis), is CGMCC in the deposit number of China Microbial Culture Preservation Commission's common micro-organisms center
NO.12779。
2. trichoderma pseudokiningii T5-1 bacterial strain according to claim 1, which is characterized in that the bacterial strain is in glucose potato
3d is cultivated on agar medium PDA, the culture dish that diameter is 9cm can be covered with, mycelia is flourishing, and aerial hyphae is fluffy dense, colourless
Element generates；The PDA culture medium composition are as follows: potato 200g/L, glucose 20g/L, agar 20g/L.
3. trichoderma pseudokiningii T5-1 bacterial strain described in claim 1 is promoting the application in Panax notoginseng Growth and root rot prevention and control,
It is characterized in that, the pathogen of the root rot is to destroy column spore bacterium (Cylindrocarpon destructans), Phytophthora cactorum bacterium
(Phtophthora cactorum), one kind of sickle-like bacteria (Fusarium solani) or more.
4. application according to claim 3, which is characterized in that use trichoderma pseudokiningii bacterium T5-1 bacterial strain sectional growing spore suspension
Liquid carries out root irrigation to Radix Notoginseng, promotes Panax notoginseng Growth.
5. application according to claim 4, which is characterized in that the trichoderma pseudokiningii bacterium T5-1 bacterial strain sectional growing spore suspension
The concentration of liquid is 1 × 104~1 × 107A conidium/mL.
6. application according to claim 4, which is characterized in that the trichoderma pseudokiningii bacterium T5-1 bacterial strain sectional growing spore suspension
The preparation method of liquid, comprising the following steps:
1) by trichoderma pseudokiningii T5-1 strain inoculated in cultivating in PDA culture medium, trichoderma pseudokiningii T5-1 bacterial strain bacterium colony is obtained；
2) 20mL sterile water is added in the culture dish of step 1), scrapes the conidium on surface, was carried out with 4 layers of sterile gauze
It filters to get trichoderma pseudokiningii T5-1 conidial suspension is arrived.
7. application according to claim 6, which is characterized in that condition of culture is 28 DEG C of dark culturing 6d in step 1).
8. application according to claim 3, which is characterized in that trichoderma pseudokiningii T5-1 bacterial strain filtrate inhibits Phytophthora cactorum bacterium
(Phtophthora cactorum) growth.
9. application according to claim 8, which is characterized in that the preparation of the trichoderma pseudokiningii T5-1 bacterial strain filtrate includes
Following steps:
1) by trichoderma pseudokiningii T5-1 strain inoculated in cultivating in PDA culture medium, trichoderma pseudokiningii T5-1 bacterial strain bacterium colony is obtained；
2) the trichoderma pseudokiningii T5-1 colony edge obtained from step 1) is beaten 20 pieces of mycelia block for taking 5mm, is seeded to containing 300mL
It in the 500mL triangular flask of PDB culture solution, after shaken cultivation 30d, is filtered using vacuum pump, obtains trichoderma pseudokiningii T5-1 bacterial strain
Filtrate；The PDB culture medium composition are as follows: potato 200g/L, glucose 20g/L.
10. application according to claim 9, which is characterized in that condition of culture is 28 DEG C of dark culturing 2d in step 1)；Step
It is rapid 2) in condition of culture be 25 DEG C, 180rpm/min.
11. application according to claim 3, which is characterized in that the volatile organic compound of trichoderma pseudokiningii T5-1 bacterial strain
Object inhibits the production spore of sickle-like bacteria (Fusarium solani) in confined conditions.
12. application according to claim 3, which is characterized in that trichoderma pseudokiningii T5-1 bacterial strain hyperparasite destroys column spore bacterium
(Cylindrocarpon destructans) and Phytophthora cactorum bacterium (Phtophthora cactorum).
13. application according to claim 12, which is characterized in that trichoderma pseudokiningii T5-1 bacterial strain is in such a way that spore adheres to
Hyperparasite destroys column spore bacterium (Cylindrocarpon destructans), and hyperparasite destroys column spore bacterium in a manner of mycelia winding
(Cylindrocarpon destructans), the hyperparasite Phytophthora cactorum bacterium (Phtophthora in a manner of formation " hook-shaped " structure
cactorum)。
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| CN108277182B (en) * | 2018-03-14 | 2022-02-18 | 云南农业大学 | Streptomyces spectabilis XWSTSP130906J234 and application thereof in prevention and treatment of panax notoginseng epidemic disease |
| CN110423696A (en) * | 2019-08-22 | 2019-11-08 | 云南农业大学 | The trichoderma strain screening technique for preventing and treating notoginseng root rot |
| CN110564650B (en) * | 2019-09-29 | 2021-01-01 | 云南农业大学 | Pseudomonas mendii and application thereof |
| CN110699301B (en) * | 2019-11-15 | 2022-11-08 | 云南大学 | Bacterial strain and application thereof in preventing and treating root rot of panax notoginseng |
| CN112877222B (en) * | 2021-04-21 | 2022-07-01 | 吉林农业大学 | Strain for antagonizing sclerotinia rot of asarum and application thereof |
| CN113303328B (en) * | 2021-05-18 | 2022-03-25 | 云南农业大学 | Application of alpha-pinene in promoting growth and inducing resistance of panax notoginseng |
| CN114644986B (en) * | 2022-03-29 | 2023-05-12 | 云南农业大学 | Trichoderma atroviride and application thereof in prevention and treatment of pseudo-ginseng diseases |
| CN115851492B (en) * | 2022-08-24 | 2024-05-10 | 云南农业大学 | Synthetic flora for preventing and treating root rot of pseudo-ginseng and application thereof |
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