CN105695346B - The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm - Google Patents
The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm Download PDFInfo
- Publication number
- CN105695346B CN105695346B CN201610262466.8A CN201610262466A CN105695346B CN 105695346 B CN105695346 B CN 105695346B CN 201610262466 A CN201610262466 A CN 201610262466A CN 105695346 B CN105695346 B CN 105695346B
- Authority
- CN
- China
- Prior art keywords
- plant
- sclerotium
- sclerotium mould
- root
- mould
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the sclerotium moulds and its preparation method and application of one plant of intoxicating plant parasitical eelworm.The bacterial strain number of the sclerotium mould is D35, is CGMCC No.12168 in the number of registering on the books of China Committee for Culture Collection of Microorganisms's common micro-organisms center.Active constituent is the plant nematode inhibitor for extracting the obtained substance for being dissolved in ethyl acetate from the culture of sclerotium mould D35 with ethyl acetate, and the corrected mortality to Meloidogyne incognita is 100%.Sclerotium mould D35 is 99% to the average inhibition of Meloidogyne incognita egg hatching.Sclerotium mould D35 solid fermentation culture under conditions of no any auxiliary agent is dosed, at 45 days to Cucumber root-knot nematode disease preventive effect up to after 83.9%, 80 day to Cucumber root-knot nematode disease preventive effect still up to 77.7%.Through sclerotium mould D35 treated cucumber seedling well developed root system, root knot is few, and plant strain growth is normal, to cucumber safety.
Description
Technical field
The invention belongs to microbial pesticide technical fields, and in particular to the sclerotium mould of one plant of intoxicating plant parasitical eelworm and
Preparation method and application.
Background technique
Many countries and regions occur and cause harm plant nematode in the world, and type is varied.In the world
Interior, the generation of plant nematode is with harm as the development of agriculture and forestry and the variation of tillage and cultivation system are got worse.According to estimating
Meter, the whole world is every year because plant nematode gives loss caused by agricultural, production of forestry to be up to 1,57,000,000,000 dollars of (Abad P, et
al.2008.Genome sequence of the metazoan plant-parasitic nematode Meloidogyne
Incognita.Nature Biotechnology, 26:909-915), and it is actual loss considerably beyond estimation, furthermore, nematode
With other biological interactions and on plant generate it is direct or indirect influence also sharply increasing.
At present to the nematode of plant pest there are about more than 3000 kinds, mainly there are root-knot nematode, cyst nematode, pine line in China
Worm etc..It is more than 3000 kinds of plants that root-knot nematode, which can endanger 114 sections, is maximum a kind of nematode of agriculturally causing harm, and disease occurs
Afterwards, general underproduction 10%-15% or so, serious up to 75% or more, or even total crop failure (A.G.Whitehead, 1998.Plant
Nematode Control.ISBN0851991882CAB International).Control of nematode at present is still with chemical prevention
It is main, but nematode body surface is not have cyto-architectural cuticula, nervous system is again undeveloped, and polypide aeration, water penetration are poor,
It is blunt to chemical reaction, it is high to people and animals' toxicity so nematocide is mostly highly toxic pesticide, pollute environment, much by
Forbid for vegetables and melon and fruit.Though have at present it is some be suitable for field of vegetables use in, less toxic nematode killing agent, kind is seldom, gives nematode
Preventing and controlling add difficulty, therefore the fungal bio-nematicide for developing highly effective and safe is extremely urgent.
Summary of the invention
The technical problem to be solved by the present invention is to how prevent and treat plant nematode.
In order to solve the above technical problems, the present invention provides one plant of sclerotium moulds.
Sclerotium mould provided by the present invention, bacterial strain number is D35, in China Committee for Culture Collection of Microorganisms
The number of registering on the books of common micro-organisms center is CGMCC No.12168.
Above-mentioned sclerotium mould D35 can be in the form of conidium, mycelia or mycelial containing conidium and/or mycelia
In the presence of.
The culture of above-mentioned sclerotium mould D35 also belongs to protection scope of the present invention.
The culture of sclerotium mould D35 provided by the present invention is to cultivate sclerotium mould D35 in microbiological culture media
The obtained substance in culture vessel, the substance include the metabolin of the sclerotium mould and the sclerotium mould.
In the culture of above-mentioned sclerotium mould D35, the microbiological culture media can be solid medium or fluid nutrient medium.
In the culture of above-mentioned sclerotium mould D35, the solid medium can be the solid culture made of brown rice and water
Base.The brown rice is that paddy sloughs the caryopsis after outer protection cortex rice husk, and interior protection cortex (pericarp, kind skin, megarchidium layer) is intact
Rice Kernel.
In order to solve the above technical problems, the present invention provides plant nematode inhibitor.
Plant nematode inhibitor provided by the present invention, its active constituent are that sclerotium mould D35 and/or sclerotium are green
The metabolin of mould D35.
The plant nematode inhibitor is concretely extracted from the culture of sclerotium mould D35 with ethyl acetate
To the substance for being dissolved in ethyl acetate.
In above-mentioned plant nematode inhibitor, the plant nematode can be root-knot nematode.
In above-mentioned plant nematode inhibitor, the root-knot nematode can be Meloidogyne incognita.
It is prepared by the metabolin of above-mentioned sclerotium mould D35 and/or sclerotium mould D35 and/or the culture of sclerotium mould D35
In plant nematode inhibitor application or preparation inhibit plant nematode endanger in cucumber medicament application also belong to
Protection scope of the present invention.
The metabolin of above-mentioned sclerotium mould D35 and/or sclerotium mould D35 and/or the culture of sclerotium mould D35 are inhibiting
Application or inhibition plant nematode in plant nematode endanger the application in cucumber and also belong to protection scope of the present invention.
In above-mentioned application, the plant nematode can be root-knot nematode.
In above-mentioned application, the root-knot nematode can be Meloidogyne incognita.
Above, in the plant nematode inhibitor, in addition to the active constituent, also contain carrier.The carrier
It commonly and can be biologically inert carrier for pesticide field.The carrier can be solid carrier or liquid-carrier；Institute
Stating solid carrier can be mineral material, vegetable material or high-molecular compound；The mineral material can be clay, talcum, kaolinite
At least one of soil, montmorillonite, white carbon, zeolite, silica and diatomite；The vegetable material can be corn flour, bean powder and shallow lake
At least one of powder；The high-molecular compound can be polyvinyl alcohol and/or polyglycols；The liquid-carrier can be organic molten
Agent, vegetable oil, mineral oil or water；The organic solvent can be decane and/or dodecane.
In the plant nematode inhibitor, above-mentioned sclerotium mould D35 can with conidium, mycelia or contain mitogenetic spore
The mycelial form of son and/or mycelia exists.
The dosage form of the plant nematode inhibitor can be a variety of dosage forms, such as liquor, emulsion, suspending agent, pulvis, particle
Agent, wettable powder or water dispersible granules.
As needed, surfactant (such as polysorbas20, Tween 80 can also be added in the plant nematode inhibitor
Deng), adhesive, stabilizer (such as antioxidant), pH adjusting agent.
It is demonstrated experimentally that active constituent be extracted from the culture of sclerotium mould D35 with ethyl acetate obtain be dissolved in second
The plant nematode inhibitor of the substance of acetoacetic ester is that 1.5g/L is dead to the correction of Meloidogyne incognita in active component content
Dying rate is 100%.Sclerotium mould D35 is 99% to the average inhibition of Meloidogyne incognita egg hatching.Sclerotium mould D35 solid
Fermentation culture medium is under conditions of no any auxiliary agent is dosed, to Cucumber root-knot nematode disease preventive effect up to 83.9%, 80 day at 45 days
Afterwards to Cucumber root-knot nematode disease preventive effect still up to 77.7%.Through sclerotium mould D35 treated cucumber seedling well developed root system, root knot is few,
Plant strain growth is normal, to cucumber safety.The metabolin of sclerotium mould D35 and/or sclerotium mould D35 can be used for plant nematode
Prevention and treatment in.
Preservation explanation
Strain name: sclerotium mould (Penicillium aff.sclerotiorum)
Strain number: D35
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation mechanism abbreviation: CGMCC
Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on April 12nd, 2016
Collection is registered on the books number: CGMCC No.12168
Detailed description of the invention
Fig. 1 is sclerotium mould D35 colonial morphology on Martin's plate.
Fig. 2 is that sclerotium mould D35 produces spore situation on Martin's plate.
Fig. 3 is sclerotium mould D35 conidiophore form.
Fig. 4 is the cucumber root that plant nematode inhibitor handles 45 days.
Fig. 5 is the cucumber root that CK handles 45 days.
Specific embodiment
The present invention is further described in detail With reference to embodiment, and the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.Experimental method in following embodiments is unless otherwise specified
Conventional method.The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The separation and identification of embodiment 1, sclerotium mould D35
1.1 strain isolation
Using dilution plate method from Chinese Wuyi Mountain, fujian soil sample isolated strains.It weighs soil sample 10g and is put into 90ml
In sterile water, 1ml 10 is drawn with 1ml Sterile pipette-1The bacterium solution of concentration shakes up in a pipe 9ml sterile water as 10-2It is dense
The bacterium solution of degree, same method are successively diluted to 10-4.By above-mentioned 10-2、10-3With 10-4Soil bacterium solution is respectively coated PDA plate
On, it is inverted in 25 DEG C of incubators and cultivates 3-5 days after drying, the fungi being separated to is transferred on the inclined-plane PDA and is cultivated, wait grow
Afterwards, it is placed in refrigerator and saves.The bacterial strain that number is D35 is taken to carry out following identifications.
The identification of 1.2 bacterial strains
1.2.1 strain morphology is observed
By bacterial strain D35 with transfer needle choose to Martin's culture medium (culture medium prescription: glucose 1g, peptone 0.5g,
KH2PO4·3H2O 0.1g、MgSO4·7H2O 0.05g, 0.1% rose-bengal solution 0.33ml, 1.5~2g of agar, distilled water
100ml, natural pH, 2% deoxycholic aicd sodium solution 2ml (sterilizing in advance is added before use), Streptomycin Solution (10 000u/ml)
0.33ml (before use be added)) plate center, it cultivates in 25 DEG C of incubators, takes a picture after 72h, and in the mitogenetic spore of microscopically observation
Son stalk and conidial form.Bacterial strain D35 bacterium colony on Martin's culture medium is rounded as can see from Figure 1, just at edge
White hypha is generated, fades to orange-yellow, is bordering on dirty-green, sporulation quantity on the more face of conidium as can see from Figure 2
Greatly.It can be seen that D35 mycelia has a tabula under Fig. 3 microscope, conidiophore top generates several wheels symmetrically or non-symmetrically small
Stalk, shaped like broom, conidium is spherical.In view of the representative configurations feature such as conidiophore and bacterium colony, Preliminary Identification D35 is mould
Belong to fungi.
1.2.2 molecular biology identification
The genomic DNA of bacterial strain D35 is extracted, -20 DEG C save backup.Using the DNA of extraction as template, amplimer is fungi
RDNA-ITS universal primer ITS1 (5 '-TCC GTA GGT GAA CCT GCG G-3 ') and ITS2 (5 '-GCT GCG TTC
TTC ATC GAT GC-3').The reaction system of PCR amplification is 2.5 μ L, dNTPs 1.5 μ L, TaqDNA of 10 × PCR Buffer
0.7 μ L of polymerase, 1.5 μ L of primer, template DNA 1.5 μ L, ddH2O 17.3 μ L, 25 μ L of total volume.The condition of pcr amplification reaction
Are as follows: 94 DEG C of initial denaturation 2min, 94 DEG C of denaturation 45S, 37 DEG C of annealing 1min, 72 DEG C of extension 2min, 35 circulations, 72 DEG C extend
10min.PCR reaction product is detected through 1% agarose gel electrophoresis, send raw work bioengineering (Beijing) limited public affairs after detection is qualified
Department's sequencing.Gained sequence is compared with sequence in GenBank database, phylogenetic evolution tree is constructed by MEGA5.0.
The rDNA-ITS sequence (sequence 1 in sequence table) that bacterial strain D35 is obtained through sequencing, is analyzed by Blast, is chosen therewith
The higher bacterial strain of homology carries out Phylogenetic Analysis using software MEGA5.0, Neighbor-Joining method constructs bacterial strain
RDNA-ITS phylogenetic tree.The result shows that D35 and Penicillium aff.sclerotiorum gather in a branch, together
Source property is 99%, therefore, in conjunction with morphological feature before, determines that bacterial strain D35 is sclerotium mould (Penicillium
aff.sclerotiorum)。
Sclerotium mould (Penicillium aff.sclerotiorum) D35 is preserved in China on April 12nd, 2016
Microbiological Culture Collection administration committee common micro-organisms center, deposit number are CGMCC No.12168.
Embodiment 2, plant root-knot nematode inhibitor and its eelworm-killing activity
The culture of sclerotium mould 1. (Penicillium aff.sclerotiorum) D35
1.1 test tube species cultures
Sclerotium mould (Penicillium aff.sclerotiorum) D35 is accessed into PDA culture medium, is cultivated at 25 DEG C
7d obtains test tube species.
Wherein, potato dextrose agar (PDA) culture medium: peeling potatoes, 200g are cut into small pieces, and boiling is added to boil
30min, 4 layers of filtered through gauze add 20g glucose, agar 17g, distilled water constant volume to 1000mL, boil mixing, under the conditions of 121 DEG C
Sterilizing 20 minutes, obtains PDA culture medium.
1.2 sclerotium moulds (Penicillium aff.sclerotiorum) D35 liquid fermentation and culture
1.1 test tube species are inoculated into the 500mL triangular flask equipped with 125mL PD fluid nutrient medium, 25 DEG C~28 DEG C
Under, shaking speed is with 200rmin-1~220rmin-1, fermentation 72h, obtain sclerotium mould (Penicillium
Aff.sclerotiorum) D35 fermentation liquid.
Wherein, potato glucose (PD) fluid nutrient medium: peeling potatoes, 200g are cut into small pieces, and boiling is added to boil
30min, 4 layers of filtered through gauze add 20g glucose, and distilled water constant volume to 1000mL boils mixing, sterilizes 20 points under the conditions of 121 DEG C
Clock obtains PD fluid nutrient medium.
1.3 solid fermentation cultures
Sclerotium mould (Penicillium aff.sclerotiorum) D35 fermentation liquid of step 1.2 is inoculated into and is equipped with
In the 500mL triangular flask of solid fermentation culture medium, every bottle of inoculation 5mL fermentation liquid, 25 DEG C of culture 40d obtain sclerotium mould
(Penicillium aff.sclerotiorum) D35 solid fermentation culture.
Wherein, solid fermentation culture medium: brown rice 80g, water 125mL sterilize 20 minutes under the conditions of 121 DEG C, obtain solid hair
Ferment culture medium.Brown rice is that paddy sloughs the caryopsis after outer protection cortex rice husk, and interior protection cortex (pericarp, kind skin, megarchidium layer) is complete
Good Rice Kernel.
2. the preparation of plant nematode inhibitor
By 1.3 sclerotium mould (Penicillium aff.sclerotiorum) D35 solid fermentation culture, glass is used
Stick is blended, every bottle of addition 300mL ethyl acetate, is set dynamic extraction on 20 DEG C of 150rpm/min shaking tables and for 24 hours, is extracted 3 times, takes out
Ethyl acetate phase is filtered to obtain, removes acetic acid in ethyl acetate phase with Rotary Evaporators (temperature: 30 DEG C, revolution: 70 turns) rotary evaporation
Ethyl ester obtains acetic acid ethyl ester extract, which is the active constituent of plant nematode inhibitor.
Acetic acid ethyl ester extract (solute) is dissolved with methanol (solvent), is configured to the solution that concentration is 1.5g/L, as
Plant nematode inhibitor.
3. the eelworm-killing activity of plant nematode inhibitor
The acquisition of 3.1 Meloidogyne incognita ovum, egg capsule and second instar larvae
Meloidogyne incognita is saved with No. 15 indoor pot vaccination ways of the good powder of susceptible tomato variety.Be inoculated with 40d after, kind
Eggplant root system has a large amount of egg capsules to occur, and root system is gently rinsed with water, egg capsule is carefully removed, is placed in 0.5% liquor natrii hypochloritis
3min is sterilized, then with aseptic water washing 3 times, is placed in the culture dish for filling a small amount of sterile water, 4 DEG C save backup.
Separately part egg capsule is taken to be placed in the culture dish for filling a small amount of sterile water, 25 DEG C of culture 4d are primary new every collecting for 24 hours
The Meloidogyne incognita second instar larvae of hatching, room temperature preservation are spare.
Old complaint is cleaned, the segment of 0.5-1cm is cut into, is put into 500mL triangular flask, 1% liquor natrii hypochloritis is added
200mL acutely shakes 5min, is collected with 200 mesh and 500 mesh mesh screen repeated flushing, is collected into pure south by 500 mesh mesh screens
Square root-knot nematode egg, 4 DEG C save backup.
Inhibiting effect of the 3.2 plant nematode inhibitor to Meloidogyne incognita second instar larvae
Plant nematode inhibitor (concentration 1.5g/L), methanol are separately added into 40 sterilized hole tissue cultures
Plate, every 100 μ L of hole, plant nematode inhibitor set 4 holes.Wherein methanol is control.It is dried up in draught cupboard, then respectively to every
100 μ L nematode suspension (content of Meloidogyne incognita second instar larvae is 20.1 ± 2.1/100 μ L) is respectively added in hole, is put into
In 25 DEG C of incubators, nemic death rate is recorded afterwards for 24 hours, calculating corrected mortality is nematicidal drug effect.Every processing is repeated 4 times.
The experimental implementation carries out in an aseptic environment.
Influence of the 1. plant nematode inhibitor of table to 2 instar larvae of Meloidogyne incognita
Note: CK: methanol control
The result shows that plant nematode inhibitor killing root-knot nematode activity is very well, to Meloidogyne incognita second instar larvae
Corrected mortality be 100% (table 1).
The inhibiting effect that 3.3 plant nematode inhibitor hatch Meloidogyne incognita egg capsule
The more consistent egg capsule of the development of 3 surface sterilizations is put into the plastic culture dish that each sterile diameter is 6cm,
It is separately added into 3mL plant nematode inhibitor (concentration 1.5g/L) and methanol.Wherein methanol is control.Test is at 25 DEG C
It carries out, each processing plastic culture dish is obturaged with sealed membrane, to reduce pollution and liquid evaporation.Microscopy respectively handles egg capsule after 7d
Hatch situation.Calculate the relative inhibition of egg capsule hatching nematode number and egg capsule hatching.Every processing is repeated 4 times.The experimental implementation exists
It is carried out under gnotobasis.
The influence that 2. plant nematode inhibitor of table hatches Meloidogyne incognita egg capsule
| Processing | Egg capsule average percentage hatch nematode (item) | Relative inhibition (%) |
| Plant nematode inhibitor | 1 | 99 |
| CK | 99 | - |
Note: CK: methanol control
The result shows that plant nematode inhibitor (concentration 1.5g/L) is able to suppress the hatching of root-knot nematode egg, it is right
Meloidogyne incognita egg hatching average inhibition is 99% (table 2).
Show sclerotium mould by the inhibiting effect test result hatched above to Meloidogyne incognita vigor and egg capsule
(Penicillium aff.sclerotiorum) D35 is the fungi of one plant of great application value, especially to Root Knot line
The nematocidal effect of worm and the inhibiting effect hatched to its egg capsule, show its good application and development prospect.
3.4 greenhouse pot cultures prevent and treat cucumber Meloidogyne incognita (Meloidogyne incognita) effect
Material to be tested and method:
Cucumber: kind is Beijing 402
Nematode: Meloidogyne incognita Meloidogyne incognita
Cucumber seedling-raising in diameter 13cm, high 10cm nutritive cube in, nursery soil be through processed no nematode garden mould and sand
Soil is mixed in 1:4 ratio.By 1.3 sclerotium mould (Penicillium aff.sclerotiorum) D35 solid fermentation culture
Object is blended with glass bar, obtains plant nematode inhibitor.Equal cucumber seedlings are inoculated with root knot line after growing 4-5 piece true leaf
Cultured nematode: being configured to the nematode suspension of 100/mL by worm, 3 apertures is uniformly inserted around plant root, by line
Worm suspension instills in aperture, and every basin is inoculated with 1500 nematodes.The same day carries out chemicals treatment, and setting is inoculated with nematode but does not have to medicament
The cucumber seedling of processing reprocesses 10 plants of cucumber seedlings as blank control (CK), 3 repetitions of each processing every time.At medicament
Reason method are as follows: plant nematode inhibitor is uniformly spread fertilizer over the fields after mixing soil, and the amount of application of plant nematode inhibitor is 100kg/
hm2, every basin pours the water of equivalent after application, is subject to impermeable, is then placed in greenhouse and is cultivated.Normal field management,
Distinguish 45 days and 80 days investigation root knot numbers after administration.Control efficiency is determined according to root knot number.
The examination of 3. sclerotium mould of table (Penicillium aff.sclerotiorum) D35 solid fermentation culture greenhouse pot culture
Test result
Note: CK: blank control
The result shows that sclerotium mould (Penicillium aff.sclerotiorum) D35 solid fermentation culture is not having
Under conditions of thering is any auxiliary agent to dose, at 45 days to Cucumber root-knot nematode disease preventive effect up to after 83.9%, 80 day to cucumber root tie lines
Parasitosis preventive effect is still up to 77.7% (table 3).Through sclerotium mould (Penicillium aff.sclerotiorum) D35, that treated is yellow
Melon seedling well developed root system, root knot is few, and plant strain growth is normal, does not find apparent phytotoxicity (Fig. 4 and Fig. 5), to cucumber safety, has good
Good application value, the concentration have reached the concentration requirement that industrialization development utilizes.
Claims (6)
1. sclerotium mould (Penicillium sclerotiorum), bacterial strain number is D35, in Chinese microorganism strain preservation
The number of registering on the books of administration committee's common micro-organisms center is CGMCC No.12168.
2. sclerotium mould described in claim 1 (Penicillium sclerotiorum) culture, be by claim 1 institute
State sclerotium mould (Penicillium sclerotiorum) in the culture vessel cultivated in microbiological culture media
Substance, the substance include the sclerotium mould (Penicillium sclerotiorum) and the sclerotium mould
(Penicillium sclerotiorum) metabolin.
3. plant nematode inhibitor, its active constituent be sclerotium mould described in claim 1 (Penicillium sclerotiorum).
4. plant nematode inhibitor according to claim 3, it is characterised in that: the plant nematode inhibitor
It is to extract the obtained substance for being dissolved in ethyl acetate from culture as claimed in claim 2 with ethyl acetate.
5. plant nematode inhibitor according to claim 3 or 4, it is characterised in that: the plant nematode is
Root-knot nematode.
6. plant nematode inhibitor according to claim 5, it is characterised in that: the root-knot nematode is Root Knot
Nematode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610262466.8A CN105695346B (en) | 2016-04-25 | 2016-04-25 | The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610262466.8A CN105695346B (en) | 2016-04-25 | 2016-04-25 | The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105695346A CN105695346A (en) | 2016-06-22 |
| CN105695346B true CN105695346B (en) | 2019-02-01 |
Family
ID=56217579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610262466.8A Active CN105695346B (en) | 2016-04-25 | 2016-04-25 | The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105695346B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520576B (en) * | 2016-11-29 | 2019-08-13 | 安徽农业大学 | A kind of sclerotium mould and its application |
| CN108130278B (en) * | 2017-12-06 | 2019-03-12 | 华南农业大学 | Purslane sclerotium mould and its preparing the application in anti-Ralstonia solanacearum drug |
| CN109535177B (en) * | 2018-11-07 | 2020-05-12 | 北京市农林科学院 | Triketone compound and application thereof |
| CN114480245B (en) * | 2022-01-18 | 2023-09-05 | 华南农业大学 | Solid fermentation spore production method of penicillium sclerotium and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445785A (en) * | 2008-12-23 | 2009-06-03 | 北京市农林科学院 | Aspergillus niger fungus capable of poisoning plant parasitic nematodes, preparation method and application thereof |
| CN102851219A (en) * | 2012-09-25 | 2013-01-02 | 中国热带农业科学院环境与植物保护研究所 | Paecilomyces lilacinus and application thereof |
| CN103910708A (en) * | 2014-02-28 | 2014-07-09 | 中山大学 | Marine fungus-derived Azaphilones compound, and preparation method and application thereof |
| CN105349434A (en) * | 2015-12-01 | 2016-02-24 | 北京市农林科学院 | Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof |
-
2016
- 2016-04-25 CN CN201610262466.8A patent/CN105695346B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445785A (en) * | 2008-12-23 | 2009-06-03 | 北京市农林科学院 | Aspergillus niger fungus capable of poisoning plant parasitic nematodes, preparation method and application thereof |
| CN102851219A (en) * | 2012-09-25 | 2013-01-02 | 中国热带农业科学院环境与植物保护研究所 | Paecilomyces lilacinus and application thereof |
| CN103910708A (en) * | 2014-02-28 | 2014-07-09 | 中山大学 | Marine fungus-derived Azaphilones compound, and preparation method and application thereof |
| CN105349434A (en) * | 2015-12-01 | 2016-02-24 | 北京市农林科学院 | Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| Penicillium species endophytic in coffee plants and ochratoxin A production;Vega FE1等;《Mycologia》;20061231;第98卷(第1期);第31-42页 * |
| 淡紫拟青霉MCWA18菌株对南方根结线虫的寄生和防治作用;高学彪等;《中国生物防治》;19981231;第14卷(第4期);第163-166页 * |
| 淡紫紫孢菌YES和粉红螺旋聚孢霉67-1组合对黄瓜根结线虫病的防效评价;张家家等;《中国生物防治学报》;20141231;第30卷(第6期);第787-794页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105695346A (en) | 2016-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102776130B (en) | Metarhizium anisopliae and application thereof | |
| CN106497831B (en) | A kind of prevention and treatment Phytophthora nicotianae disease composite bacteria agent and its preparation method and application | |
| CN101575574A (en) | Trichoderma harzianum composite bacteria culture and application of trichoderma harzianum composite bacteria culture in aspect of plant protection | |
| CN106119155B (en) | For preventing and treating the bacterial strain WXX-2 and microbial inoculum of peanut root rot | |
| CN106591157B (en) | The preparation and application of the Tabin aspergillus and its metabolite of one plant of disease prevention growth-promoting | |
| CN105695346B (en) | The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm | |
| CN102363750A (en) | Insecticidal fungus and its application | |
| CN108034601A (en) | One bacillus amyloliquefaciens and its application | |
| CN105039167A (en) | Beauveria bassiana DSXJ-07 and application thereof | |
| CN102191184B (en) | Biocontrol endophytic fungi-Alternaria alternata | |
| CN105238709A (en) | Marine actinomycete strain with meloidogyne insecticidal activity and application of marine actinomycete strain | |
| CN105154339A (en) | Trichoderma viride strain and application thereof | |
| CN105132296A (en) | Hook-like trichoderma strain and application thereof | |
| CN103451111B (en) | Saline-alkali tolerant trichoderma harzianum and application thereof | |
| CN103451112B (en) | Saline-alkali tolerant trichoderma longibrachiatum and application thereof | |
| CN105385623A (en) | Actinomycete strain JSX-1 for preventing and curing phytophthora sojae and fungicide thereof | |
| CN105112304B (en) | A kind of porous smoke pipe bacterium Gao Shi 15 and its preparation for preventing and treating vegetables root disease | |
| CN104342388B (en) | Streptomycete bacterial strain and prevent and treat tomato wilt use in conjunction | |
| CN111334458B (en) | Biocontrol actinomycetes and application thereof in prevention and control of ginger stem basal rot or soybean epidemic disease | |
| CN105695376B (en) | One plant of simple bacillus for killing plant root-knot nematode and its application | |
| CN106148221A (en) | The stenotrophomonas antibacterial of one strain induction Semen arachidis hypogaeae root-knot nematode resistant | |
| CN104805022A (en) | Fusarium oxysporum as well as microbial inoculum and application thereof | |
| CN104531543A (en) | Tephrosia purpurea endophytic fungus TPL25 and application thereof in plant disease prevention and treatment | |
| CN104593266B (en) | A kind of Endophytic Fungi in Tomato intertexture branch top spore and its application in tomato root-knot eelworm diease occurrence is anti- | |
| CN104560740B (en) | Metarhizium anisopliae and its application of one plant of preventing and treating oil tea as larva |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |