CN107173108A - Strawberry test tube seedling rooting culture matrix and its application - Google Patents
Strawberry test tube seedling rooting culture matrix and its application Download PDFInfo
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Abstract
The present invention relates to technical field of plant culture, and in particular to a kind of strawberry test tube seedling rooting culture matrix and its application.Matrix includes：Mass ratio is 1:1 fertile soil and the mixture of the dregs of a decoction；Wherein, the dregs of a decoction are from the product using Chinese herbal medicine as raw material after extraction, and Chinese herbal medicine includes radix scutellariae, ginkgo leaf, glossy privet seed, cordate houttuynia, polygonum capitatum and sealwort, and based on ginkgo leaf.The Strawberry Seedlings number of blade that the rooting culture matrix provided using the present invention is obtained is more, and leaf color is green, and root system enriches, and packing is fabulous, can not only significantly improve the transplanting survival rate of strawberry test tube seedling, and the fruit yield of strawberry is significantly improved, and ill number is drastically reduced；In addition, the present invention uses broad-leaved fertile soil and the dregs of a decoction as raw material, and it is simple to operate, with low cost, it is significant to improving planting household life, strawberry production level can be promoted to reach a new high.
Description
Technical field
The present invention relates to technical field of plant culture, and in particular to a kind of strawberry test tube seedling rooting culture matrix and its should
With.
Background technology
Strawberry be called the foreign certain kind of berries, the certain kind of berries etc., the category rose family, Fragaria, herbaceos perennial.Strawberry fruit is delicious red tender, fruit
Meat succulence, containing special strong fruit aroma, and rich in vitamin C, vitamin A, carrotene etc., there is higher nutriture value
Value and medical value.Latest research is pointed out：Contain a kind of amine substance in strawberry, to treatment leukaemia and aplastic
Anaemia has certain effect.Therefore, strawberry is as a kind of fruit in season suitable for people of all ages, and market price is high, demand is big.
However, Strawberry Virus as influence strawberry production Major Diseases, generally result in the strawberry underproduction 30%~
50%.Contaminating the virosis of strawberry at present mainly has four kinds：Strawberry mottle virus, strawberry crinkle virus, strawberry be light and yellow edge
Poison and strawberry veinbanding virus.Investigated according to project team：The plantation of Guizhou strawberry, which is started from, takes charge of under late 1990s, Majiang, spends
Bridge, Huaxi, Guiyang has fiery village the custom of plantation strawberry over the years, there is good basis, 2000 Annual planting areas up to more than 20,000 mus,
Per per mu yield value up to 5000~10000 yuan.But in recent years due to virus infection, cause yield to glide year by year, serious contusion
The plantation enthusiasm of certain kind of berries agriculture, cultivated area is reduced year by year.Only lower department's flower band of bridge one, cultivated area reduces nearly 1000 mu；One
The preferable kind of a little qualities (such as the spring is fragrant) is also due to yielding poorly and being eliminated.
Based on this, the extensive pass of people is obtained using the effective way of tissue culture technique virus-free, raising yield of strawberry
Note.However, due to scientific research, production circulation and the serious disconnection in the field such as financial cost, shift to new management mechanisms not smooth, detoxic seedling is pushed away
Wide application is made slow progress.Therefore, study a kind of new, cost-effective and be easy to the method popularized particularly important.
The content of the invention
For defect of the prior art, the present invention is intended to provide a kind of strawberry test tube seedling rooting culture matrix and its should
With.The rooting culture matrix provided using the present invention, can not only significantly improve the transplanting survival rate of strawberry test tube seedling, Er Qiecao
The fruit yield of the certain kind of berries is significantly improved, and ill number is drastically reduced；In addition, the rooting culture matrix application of the present invention is convenient, cost is low
It is honest and clean, it is easy to popularize, significantly improve the enthusiasm for production at strawberry cultivating family.
Therefore, the present invention provides following technical scheme：
In a first aspect, the present invention provides a kind of test tube seedling rooting culture matrix, matrix includes：Mass ratio is 1:1 humic
The mixture of soil and the dregs of a decoction；Wherein, the dregs of a decoction are from the product using Chinese herbal medicine as raw material after extraction, and Chinese herbal medicine includes Huang
A kind of reed mentioned in ancient books, ginkgo leaf, glossy privet seed, cordate houttuynia, polygonum capitatum and sealwort.
The present invention further embodiment in, each raw material components in Chinese herbal medicine by weight, including：Radix scutellariae 3
~5 parts by weight, the parts by weight of ginkgo leaf 60~100, the parts by weight of glossy privet seed 6~15, the parts by weight of cordate houttuynia 1~3, the weight of polygonum capitatum 5~8
Measure part and the parts by weight of sealwort 10~15.
In the further embodiment of the present invention, each raw material components in Chinese herbal medicine also include：The parts by weight of bitter buckwheat 2~6,
The parts by weight of Gorgon fruit 1~2 and the parts by weight of grass-leaved sweetflag 3~5.
In the further embodiment of the present invention, the preparation method of the dregs of a decoction includes：S101：Each raw material components are cleaned,
Crushed after drying, afterwards by glossy privet seed and polygonum capitatum water retting, the pH value of product is 6.5~6.8, Zhi Houjia after regulation dipping
Water boiling and extraction；S102：Surplus stock component is well mixed, extracted using ethanol water；S103：Extracted decocting
Product after being extracted with ethanol water is well mixed, and is filtered afterwards and is collected filter residue, obtains the dregs of a decoction.
In the further embodiment of the present invention, in addition to：Filter residue in S103 is subjected to microwave drying, obtained afterwards
The dregs of a decoction；Wherein, the condition of microwave drying is：Vacuum is 0.07MPa~0.09MPa, and temperature change is：With 1~2 DEG C of cooling
Speed is cooled to 5 DEG C~10 DEG C, is incubated 10min~20min；65 DEG C~75 DEG C are warming up to 5~8 DEG C of heating rate afterwards,
It is incubated 80min~100min.
In the further embodiment of the present invention, in S101, add water to cook in extraction, the addition quality of water is glossy privet seed
With 5~10 times of polygonum capitatum gross mass, extraction time be 80min~150min；In S102, the quality percentage of ethanol water is dense
Spend for 15%~20%, Extracting temperature is 60 DEG C~70 DEG C, extraction time is 60min~200min.
In the further embodiment of the present invention, fertile soil selects broad-leaved fertile soil.Specifically, the broad-leaved in the present invention
Fertile soil, can be prepared using conventional method：The things such as fallen leaves, the weeds of broad-leaved or coniferous tree are collected, heap enters rectangle hole
It is interior, one layer of leaf is first put during composting；One layer of garden mould is put again, is so stacked repeatedly after several layers, then pours a small amount of sewage, finally on top
The things such as the garden mould of the cm thick of portion's lid last layer about 10；Late spring, the midsummer in the coming year respectively open once, stir and smash deposit to pieces, then
Heap is good as it is；Warmer climate area, can become thoroughly decomposed greatly to these deposits of season in late autumn.It can now dig out, further
Used after smashing sieving to pieces.It is of course also possible to directly be commercially available.
Second aspect, the test tube seedling rooting culture matrix that the present invention is provided answering in strawberry detoxification tissue culture quick-breeding method
With.
In the further embodiment of the present invention, application process comprises the following steps：S201：Choose crawling for strawberry
Crawl stem is heat-treated and disinfected as explant successively；S202：Explant after disinfecting peels off stem apex, so
Induction obtains adventitious bud afterwards；S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling；S204：Will examination
Pipe seedling plant division, which is inoculated in, lures root culture medium to carry out culture of rootage；S205：Test tube transplantation of seedlings to test tube seedling after culture of rootage is tamed and dociled
Change in transplanting medium, culture is managed afterwards, Strawberry Seedlings are obtained.
In the further embodiment of the present invention, in S205, the time of management culture is 40~60 days, and management culture
Specifically include：After transplanting 7~10 days, blade face is sprayed with nutrient solution, is sprayed weekly twice；Whole management incubation, every 10~
Spray within 15 days an insect prevention liquid.
The above-mentioned technical proposal of the present invention has advantages below compared with prior art：
(1) applicant has found by many experiments：The rooting culture matrix provided using the present invention, not only can significantly be carried
The transplanting survival rate of high strawberry test tube seedling, and the fruit yield of strawberry significantly improves, and ill number is drastically reduced；In addition, this hair
Bright rooting culture matrix application is convenient, and with low cost, it is easy to popularize, the production for significantly improving strawberry cultivating family is positive
Property.
(2) the Strawberry Seedlings number of blade that the rooting culture matrix culture provided using the present invention is obtained is more, and leaf color is green, and root system is rich
Richness, packing is fabulous, and strawberry resistance is significantly increased, and strawberry fruit yield can improve 30%~50%；And use broad-leaved humic
Soil and the dregs of a decoction are simple to operate, with low cost as raw material, significant to improving planting household life, strawberry can be promoted to give birth to
Production level reaches a new high.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
The embodiment to technical solution of the present invention is described in detail below.Following examples are only used for clearer
Explanation technical scheme, therefore be only used as example, and can not be limited the scope of the invention with this.
Experimental method in following embodiments, is conventional method unless otherwise specified.
Test material used, is to be commercially available from conventional reagent shop unless otherwise specified in following embodiments.
Quantitative test in following examples, is respectively provided with three repetition experiments, and data are the average value of three repetition experiments
Or mean+SD.
The present invention provides a kind of test tube seedling rooting culture matrix, and matrix includes：Mass ratio is 1:1 fertile soil and the dregs of a decoction
Mixture；Wherein, the dregs of a decoction are from product using Chinese herbal medicine as raw material after extraction, and Chinese herbal medicine include radix scutellariae, ginkgo leaf,
Glossy privet seed, cordate houttuynia, polygonum capitatum and sealwort.Wherein, each raw material components in Chinese herbal medicine by weight, including：Radix scutellariae 3~5
Parts by weight, the parts by weight of ginkgo leaf 60~100, the parts by weight of glossy privet seed 6~15, the parts by weight of cordate houttuynia 1~3, the weight of polygonum capitatum 5~8
Part and the parts by weight of sealwort 10~15.
Preferably, each raw material components in Chinese herbal medicine also include：The parts by weight of bitter buckwheat 2~6, the parts by weight of Gorgon fruit 1~2 and stone
The parts by weight of calamus 3~5.
Preferably, the preparation method of the dregs of a decoction includes：
S101：Crushed after each raw material components are cleaned, dried, afterwards by glossy privet seed and polygonum capitatum water retting, regulation leaching
The pH value of product is 6.5~6.8 after stain, and extraction is added water to cook afterwards.Wherein, add water to cook in extraction, the addition quality of water is
5~10 times of glossy privet seed and polygonum capitatum gross mass, extraction time is 80min~150min.
S102：Surplus stock component is well mixed, extracted using ethanol water.Wherein, ethanol water
Mass percentage concentration is 15%~20%, and Extracting temperature is 60 DEG C~70 DEG C, and extraction time is 60min~200min.
S103：It will decoct to extract and be well mixed with the product after ethanol water extraction, filter afterwards and collect filter residue, obtain
To the dregs of a decoction.Wherein, also include in S103：Filter residue is subjected to microwave drying, the dregs of a decoction are obtained afterwards；Wherein, the condition of microwave drying
For：Vacuum is 0.07MPa~0.09MPa, and temperature change is：5 DEG C~10 DEG C, insulation are cooled to 1~2 DEG C of rate of temperature fall
10min~20min；65 DEG C~75 DEG C are warming up to 5~8 DEG C of heating rate afterwards, 80min~100min is incubated.
In addition, the test tube seedling rooting culture matrix that the present invention is provided is applied in strawberry detoxification tissue culture quick-breeding method, tool
Body comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively.
S202：Explant after disinfecting peels off stem apex, and then induction obtains adventitious bud.
S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling.
S204：Test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage.
S205：By the test tube transplantation of seedlings after culture of rootage into test tube seedling rooting culture matrix, culture is managed afterwards,
Obtain Strawberry Seedlings.Wherein, the time of management culture is 40~60 days, and management culture is specifically included：After transplanting 7~10 days, use
Nutrient solution sprays blade face, sprays weekly twice；Whole management incubation, sprays an insect prevention liquid in every 10~15 days.
Illustrated with reference to embodiment：
Embodiment one
The present invention provides a kind of strawberry detoxification tissue culture quick-breeding method, comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively.
S202：Explant after disinfecting peels off stem apex, and then induction obtains adventitious bud.
S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling.
S204：Test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage.
S205：By the test tube transplantation of seedlings after culture of rootage into test tube seedling rooting culture matrix, culture is managed afterwards
50 days, and after transplanting 10 days, spray blade face with nutrient solution twice a week；Whole management incubation, is sprayed once for every 15 days
Insect prevention liquid, finally gives Strawberry Seedlings.
Wherein, it is 1 that matrix, which includes mass ratio,:1 broad-leaved fertile soil and the mixture of the dregs of a decoction；The dregs of a decoction are selected to be made with Chinese herbal medicine
The product for being raw material after extraction, and each raw material components in Chinese herbal medicine are by weight, including：The parts by weight of radix scutellariae 3, ginkgo
The parts by weight of leaf 100, the parts by weight of glossy privet seed 6, the parts by weight of cordate houttuynia 3, the parts by weight of polygonum capitatum 5 and the parts by weight of sealwort 15.The dregs of a decoction
Preparation method includes：S101：Crush, afterwards by glossy privet seed and polygonum capitatum water retting, adjust after each raw material components are cleaned, dried
The pH value of product is 6.5 after section dipping, extraction 150min is added water to cook afterwards, the addition quality of water is that glossy privet seed and polygonum capitatum are total
5 times of quality；S102：Surplus stock component is well mixed, use mass percentage concentration for 20% ethanol water in temperature
Spend to extract 60min under conditions of 70 DEG C；S103：It will decoct to extract and be well mixed with the product after ethanol water extraction, it
After filter and collect filter residue, in vacuum be 0.07MPa by filter residue, temperature change is：5 DEG C are cooled to 2 DEG C of rate of temperature fall,
It is incubated 20min；65 DEG C are warming up to 5 DEG C of heating rate afterwards, 100min is incubated, finally gives the dregs of a decoction.
Embodiment two
The present invention provides a kind of strawberry detoxification tissue culture quick-breeding method, comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively.
S202：Explant after disinfecting peels off stem apex, and then induction obtains adventitious bud.
S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling.
S204：Test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage.
S205：By the test tube transplantation of seedlings after culture of rootage into test tube seedling rooting culture matrix, culture is managed afterwards
40 days, and after transplanting 7 days, spray blade face with nutrient solution twice a week；Whole management incubation, sprays and once prevents for every 10 days
Worm liquid, finally gives Strawberry Seedlings.
Wherein, it is 1 that matrix, which includes mass ratio,:1 broad-leaved fertile soil and the mixture of the dregs of a decoction；The dregs of a decoction are selected to be made with Chinese herbal medicine
The product for being raw material after extraction, and each raw material components in Chinese herbal medicine are by weight, including：The parts by weight of radix scutellariae 5, ginkgo
The parts by weight of leaf 60, the parts by weight of glossy privet seed 15, the parts by weight of cordate houttuynia 1, the parts by weight of polygonum capitatum 8 and the parts by weight of sealwort 10.The dregs of a decoction
Preparation method includes：S101：Crush, afterwards by glossy privet seed and polygonum capitatum water retting, adjust after each raw material components are cleaned, dried
The pH value of product is 6.8 after section dipping, extraction 80min is added water to cook afterwards, the addition quality of water is that glossy privet seed and polygonum capitatum are total
10 times of quality；S102：Surplus stock component is well mixed, use mass percentage concentration for 15% ethanol water in temperature
Spend to extract 200min under conditions of 60 DEG C；S103：It will decoct to extract and be well mixed with the product after ethanol water extraction, it
After filter and collect filter residue, in vacuum be 0.09MPa by filter residue, temperature change is：10 are cooled to 1 DEG C of rate of temperature fall
DEG C, it is incubated 10min；75 DEG C are warming up to 8 DEG C of heating rate afterwards, 80min is incubated, finally gives the dregs of a decoction.
Embodiment three
The present embodiment changes relevant parameter on the basis of embodiment one and formed.
The present invention provides a kind of strawberry detoxification tissue culture quick-breeding method, comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively.
S202：Explant after disinfecting peels off stem apex, and then induction obtains adventitious bud.
S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling.
S204：Test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage.
S205：By the test tube transplantation of seedlings after culture of rootage into test tube seedling rooting culture matrix, culture is managed afterwards
50 days, and after transplanting 10 days, spray blade face with nutrient solution twice a week；Whole management incubation, is sprayed once for every 15 days
Insect prevention liquid, finally gives Strawberry Seedlings.
Wherein, it is 1 that matrix, which includes mass ratio,:1 broad-leaved fertile soil and the mixture of the dregs of a decoction；The dregs of a decoction are selected to be made with Chinese herbal medicine
The product for being raw material after extraction, and each raw material components in Chinese herbal medicine are by weight, including：The parts by weight of radix scutellariae 3, ginkgo
The parts by weight of leaf 100, the parts by weight of glossy privet seed 6, the parts by weight of cordate houttuynia 3, the parts by weight of polygonum capitatum 5, the parts by weight of sealwort 15, the weight of bitter buckwheat 6
Part, the parts by weight of Gorgon fruit 1 and the parts by weight of grass-leaved sweetflag 5.The preparation method of the dregs of a decoction includes：S101：Each raw material components are cleaned, dried
After crush, afterwards by glossy privet seed and polygonum capitatum water retting, regulation dipping after product pH value be 6.5, add water to cook carry afterwards
150min is taken, the addition quality of water is 5 times of glossy privet seed and polygonum capitatum gross mass；S102：Surplus stock component is well mixed,
It in temperature is to extract 60min under conditions of 70 DEG C for 20% ethanol water to use mass percentage concentration；S103：Decoction is carried
Take the product after being extracted with ethanol water to be well mixed, filter afterwards and collect filter residue, be in vacuum by filter residue
0.07MPa, temperature change is：5 DEG C are cooled to 2 DEG C of rate of temperature fall, 20min is incubated；Heated up afterwards with 5 DEG C of heating rate
To 65 DEG C, 100min is incubated, the dregs of a decoction are finally given.
Example IV
The present embodiment changes relevant parameter on the basis of embodiment two and formed.
The present invention provides a kind of strawberry detoxification tissue culture quick-breeding method, comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively.
S202：Explant after disinfecting peels off stem apex, and then induction obtains adventitious bud.
S203：Obtained adventitious bud will be induced to carry out many for shoot proliferation, obtain test tube seedling.
S204：Test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage.
S205：By the test tube transplantation of seedlings after culture of rootage into test tube seedling rooting culture matrix, culture is managed afterwards
40 days, and after transplanting 7 days, spray blade face with nutrient solution twice a week；Whole management incubation, sprays and once prevents for every 10 days
Worm liquid, finally gives Strawberry Seedlings.
Wherein, it is 1 that matrix, which includes mass ratio,:1 broad-leaved fertile soil and the mixture of the dregs of a decoction；The dregs of a decoction are selected to be made with Chinese herbal medicine
The product for being raw material after extraction, and each raw material components in Chinese herbal medicine are by weight, including：The parts by weight of radix scutellariae 5, ginkgo
The parts by weight of leaf 60, the parts by weight of glossy privet seed 15, the parts by weight of cordate houttuynia 1, the parts by weight of polygonum capitatum 8, the parts by weight of sealwort 10, the weight of bitter buckwheat 2
Part, the parts by weight of Gorgon fruit 2 and the parts by weight of grass-leaved sweetflag 3.The preparation method of the dregs of a decoction includes：S101：Each raw material components are cleaned, dried
After crush, afterwards by glossy privet seed and polygonum capitatum water retting, regulation dipping after product pH value be 6.8, add water to cook carry afterwards
80min is taken, the addition quality of water is 10 times of glossy privet seed and polygonum capitatum gross mass；S102：Surplus stock component is well mixed,
It in temperature is to extract 200min under conditions of 60 DEG C for 15% ethanol water to use mass percentage concentration；S103：It will decoct
Extract the product after extracting with ethanol water to be well mixed, filter afterwards and collect filter residue, be in vacuum by filter residue
0.09MPa, temperature change is：10 DEG C are cooled to 1 DEG C of rate of temperature fall, 10min is incubated；Afterwards with 8 DEG C of heating rate liter
Temperature is incubated 80min, finally gives the dregs of a decoction to 75 DEG C.
In addition, the advantage in order to further illustrate technical solution of the present invention, sets following comparative example.It should be noted that
In following comparative examples, in addition to the test tube seedling rooting culture matrix in S205 is different, the equal be the same as Example one of remaining parameter.
Comparative example one
Test tube seedling rooting culture matrix selects pine soil.
Comparative example two
Test tube seedling rooting culture matrix selects broad-leaved fertile soil.
Comparative example three
Test tube seedling rooting culture matrix selects garden mould.
Comparative example four
Test tube seedling rooting culture matrix selects the dregs of a decoction, a kind of dregs of a decoction of the component be the same as Examples of the dregs of a decoction.
In addition, the Strawberry Seedlings that various embodiments of the present invention and each comparative example are obtained carry out routine observation record, system evaluation
Its upgrowth situation.The Strawberry Seedlings finally given to each embodiment and comparative example carry out observed and recorded, and every kind of matrix transplants 200
Strain, in triplicate, routine observation record；Concrete outcome is as shown in Table 1 and Table 2.In table 2, for strawberry plant height and yield, to adopt
The Strawberry Seedlings growing way obtained with conventional medium makees benchmark, counts the strawberry growing way of each embodiment and comparative example.
Influence list one of the different transplanting mediums of table 1 to Strawberry Seedlings upgrowth situation
Influence list two of the different transplanting mediums of table 2 to Strawberry Seedlings upgrowth situation
| Plant height increase/% | Strawberry volume increase/% | Illness rate/% | |
| Embodiment one | 26.58 | 55.98 | 1.21 |
| Embodiment two | 25.35 | 55.26 | 1.30 |
| Embodiment three | 30.06 | 67.79 | 0.59 |
| Example IV | 31.58 | 66.96 | 0.62 |
It should be noted that situation about enumerating except embodiment one to example IV, the species of other raw material components and match somebody with somebody
It is more also possible than the condition and parameter in, preparation process etc..
The rooting culture matrix provided using the present invention, can not only significantly improve the transplanting survival rate of strawberry test tube seedling,
And the fruit yield of strawberry is significantly improved, ill number is drastically reduced；In addition, the rooting culture matrix application of the present invention is convenient,
It is with low cost, it is easy to popularize, significantly improve the enthusiasm for production at strawberry cultivating family.
In the description of the invention, it is to be understood that term " first ", " second " are only used for describing purpose, and can not
It is interpreted as indicating or implies relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, define " the
One ", one or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the invention,
" multiple " are meant that two or more, unless otherwise specifically defined.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or the spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the not be the same as Example or the feature of example and non-be the same as Example or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
1. a kind of test tube seedling rooting culture matrix, it is characterised in that the matrix includes：Mass ratio is 1:1 fertile soil and medicine
The mixture of slag；
Wherein, the dregs of a decoction are from the product using Chinese herbal medicine as raw material after extraction, and the Chinese herbal medicine includes radix scutellariae, silver
Apricot leaf, glossy privet seed, cordate houttuynia, polygonum capitatum and sealwort.
2. test tube seedling rooting culture matrix according to claim 1, it is characterised in that：
Each raw material components in the Chinese herbal medicine by weight, including：The parts by weight of radix scutellariae 3~5, the weight of ginkgo leaf 60~100
Part, the parts by weight of glossy privet seed 6~15, the parts by weight of cordate houttuynia 1~3, the parts by weight of polygonum capitatum 5~8 and the parts by weight of sealwort 10~15.
3. test tube seedling rooting culture matrix according to claim 2, it is characterised in that：
Each raw material components in the Chinese herbal medicine also include：The parts by weight of bitter buckwheat 2~6, the parts by weight of Gorgon fruit 1~2 and grass-leaved sweetflag 3~
5 parts by weight.
4. test tube seedling rooting culture matrix according to claim 1, it is characterised in that the preparation method bag of the dregs of a decoction
Include：
S101：Crushed after each raw material components are cleaned, dried, afterwards by glossy privet seed and polygonum capitatum water retting, adjust the leaching
The pH value of product is 6.5~6.8 after stain, and extraction is added water to cook afterwards；
S102：Surplus stock component is well mixed, extracted using ethanol water；
S103：The product decocted after extraction and ethanol water extraction is well mixed, filters afterwards and collects filter
Slag, obtains the dregs of a decoction.
5. test tube seedling rooting culture matrix according to claim 4, it is characterised in that also include：
Filter residue in the S103 is subjected to microwave drying, the dregs of a decoction are obtained afterwards；
Wherein, the condition of the microwave drying is：Vacuum is 0.07MPa~0.09MPa, and temperature change is：With 1~2 DEG C
Rate of temperature fall is cooled to 5 DEG C~10 DEG C, is incubated 10min~20min；Afterwards 65 DEG C~75 are warming up to 5~8 DEG C of heating rate
DEG C, it is incubated 80min~100min.
6. test tube seedling rooting culture matrix according to claim 4, it is characterised in that：
It is described to add water to cook in extraction in the S101, the addition quality of water for the glossy privet seed and polygonum capitatum gross mass 5~
10 times, extraction time is 80min~150min；
In the S102, the mass percentage concentration of the ethanol water is 15%~20%, and Extracting temperature is 60 DEG C~70 DEG C,
Extraction time is 60min~200min.
7. the test tube seedling rooting culture matrix according to any one of claim 1~6, it is characterised in that：
The fertile soil selects broad-leaved fertile soil.
8. application of any one of the claim 1~7 test tube seedling rooting culture matrix in strawberry detoxification tissue culture quick-breeding method.
9. application of the test tube seedling rooting culture matrix according to claim 8 in strawberry detoxification tissue culture quick-breeding method, its
It is characterised by, comprises the following steps：
S201：The stolon of strawberry is chosen as explant, is heat-treated and is disinfected successively；
S202：Stem apex is peeled off from the explant after described disinfect, then induction obtains adventitious bud；
S203：Induce obtained adventitious bud to carry out more for shoot proliferation by described, obtain test tube seedling；
S204：The test tube seedling plant division is inoculated in and lures root culture medium to carry out culture of rootage；
S205：By the test tube transplantation of seedlings after the culture of rootage into the test tube seedling rooting culture matrix, it is managed afterwards
Culture, obtains Strawberry Seedlings.
10. application of the test tube seedling rooting culture matrix according to claim 9 in strawberry detoxification tissue culture quick-breeding method, its
It is characterised by：
In the S205, the time of the management culture is 40~60 days, and the management culture is specifically included：Transplant 7~10
After it, blade face is sprayed with nutrient solution, is sprayed weekly twice；Whole management incubation, sprays an insect prevention liquid in every 10~15 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243695A (en) * | 2018-01-19 | 2018-07-06 | 贵州省生物研究所 | A kind of Chinese actinidia root maize ear rot pesticide control and its application method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102113462A (en) * | 2011-03-09 | 2011-07-06 | 国家林业局泡桐研究开发中心 | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof |
| CN102726179A (en) * | 2011-04-13 | 2012-10-17 | 林光德 | Cultivation method of red-color strawberries |
| CN106106094A (en) * | 2016-06-30 | 2016-11-16 | 浙江森禾种业股份有限公司 | Pohle happy pieris container seedling plantation substrate |
| CN106688415A (en) * | 2016-11-30 | 2017-05-24 | 杨立新 | Method for cultivating selenium-zinc-enriched forest product |
| CN106818490A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving subprostrate sophora tissue-cultured seedling flavone compound and polysaccharide |
-
2017
- 2017-06-16 CN CN201710456767.9A patent/CN107173108A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946702A (en) * | 2010-08-13 | 2011-01-19 | 北京金六环农业园 | Special medium for tissue culture of strawberry stem tip and method thereof for producing detoxification seedlings |
| CN102113462A (en) * | 2011-03-09 | 2011-07-06 | 国家林业局泡桐研究开发中心 | Functional type gingko champignon cultivated by using medicinal plant residues and production method thereof |
| CN102726179A (en) * | 2011-04-13 | 2012-10-17 | 林光德 | Cultivation method of red-color strawberries |
| CN106106094A (en) * | 2016-06-30 | 2016-11-16 | 浙江森禾种业股份有限公司 | Pohle happy pieris container seedling plantation substrate |
| CN106688415A (en) * | 2016-11-30 | 2017-05-24 | 杨立新 | Method for cultivating selenium-zinc-enriched forest product |
| CN106818490A (en) * | 2017-04-08 | 2017-06-13 | 广西科技大学鹿山学院 | A kind of culture medium for improving subprostrate sophora tissue-cultured seedling flavone compound and polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 石晓东、高润梅: "《植物组织培养》", 28 February 2009, 中国农业科学技术出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108243695A (en) * | 2018-01-19 | 2018-07-06 | 贵州省生物研究所 | A kind of Chinese actinidia root maize ear rot pesticide control and its application method |
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