CN103026968B - 一种铁皮石斛种苗工厂化生产方法 - Google Patents
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- CN103026968B CN103026968B CN201210590755.2A CN201210590755A CN103026968B CN 103026968 B CN103026968 B CN 103026968B CN 201210590755 A CN201210590755 A CN 201210590755A CN 103026968 B CN103026968 B CN 103026968B
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106962165A (zh) * | 2017-05-22 | 2017-07-21 | 东莞市睿绅生物技术有限公司 | 一种可移动的铁皮石斛培养基的制作方法及一体化育苗方法 |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103202234B (zh) * | 2013-04-28 | 2014-05-14 | 陕西师范大学 | 铁皮石斛的快速繁殖方法 |
CN103355167A (zh) * | 2013-07-17 | 2013-10-23 | 红河州巨丰生物科技有限公司 | 一种以塑料奶茶杯为组培容器的铁皮石斛组培方法 |
CN103583359A (zh) * | 2013-10-24 | 2014-02-19 | 湖南农业大学 | 铁皮石斛兰的组织培养方法 |
CN103931495A (zh) * | 2014-03-26 | 2014-07-23 | 铜仁市碧江区宝泓生态农庄有限公司 | 一种铁皮石斛组培繁育方法 |
CN104303765B (zh) * | 2014-10-10 | 2017-05-31 | 泉州惠安长圣生物科技有限公司 | 石斛的高产种植方法 |
CN104304008A (zh) * | 2014-10-10 | 2015-01-28 | 江苏神草生物科技有限公司 | 一种石斛不定芽诱导培养基 |
CN105145298A (zh) * | 2015-07-30 | 2015-12-16 | 黄旭胜 | 一种铁皮石斛盆栽方法 |
CN105284479B (zh) * | 2015-11-23 | 2018-05-15 | 铜仁市金农绿色农业科技有限公司 | 一种抗寒铁皮石斛组培苗的培育方法 |
CN106472306B (zh) * | 2016-09-30 | 2018-09-25 | 南京仙草堂生物科技有限公司 | 一种始兴石斛优质种苗无性克隆快速繁殖方法 |
CN107494218B (zh) * | 2017-07-18 | 2020-04-28 | 霍山县茧丝绸有限公司 | 一种石斛的高芽育苗方法 |
CN108684524A (zh) * | 2018-05-16 | 2018-10-23 | 芜湖市三山区绿色食品产业协会 | 铁皮石斛的组织培养方法 |
CN108812311A (zh) * | 2018-06-06 | 2018-11-16 | 周庆友 | 一种栝楼种苗快繁的方法 |
CN112088760A (zh) * | 2020-07-28 | 2020-12-18 | 灵山县山霖生物技术有限公司 | 一种铁皮石斛的育苗方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6168952B1 (en) * | 1998-08-04 | 2001-01-02 | Korea Kumho Petrochemical Co., Ltd. | Method for producing flowering orchids in vitro |
CN1961632A (zh) * | 2006-12-08 | 2007-05-16 | 蔡仲玉 | 一种铁皮石斛栽培方法 |
CN101258835A (zh) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | 铁皮石斛优质种苗快速繁殖方法 |
CN101822211A (zh) * | 2009-09-03 | 2010-09-08 | 昆明优利丰园科技开发有限公司 | 云南铁皮石斛的一种组织培育方法 |
CN102613086A (zh) * | 2012-03-31 | 2012-08-01 | 南京农业大学 | 一种铁皮石斛无激素组织培养的方法 |
CN102657083A (zh) * | 2012-05-04 | 2012-09-12 | 福建农林大学 | 一种铁皮石斛苗茶及其制备方法 |
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2012
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Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6168952B1 (en) * | 1998-08-04 | 2001-01-02 | Korea Kumho Petrochemical Co., Ltd. | Method for producing flowering orchids in vitro |
CN1961632A (zh) * | 2006-12-08 | 2007-05-16 | 蔡仲玉 | 一种铁皮石斛栽培方法 |
CN101258835A (zh) * | 2008-04-23 | 2008-09-10 | 昆明理工大学 | 铁皮石斛优质种苗快速繁殖方法 |
CN101822211A (zh) * | 2009-09-03 | 2010-09-08 | 昆明优利丰园科技开发有限公司 | 云南铁皮石斛的一种组织培育方法 |
CN102613086A (zh) * | 2012-03-31 | 2012-08-01 | 南京农业大学 | 一种铁皮石斛无激素组织培养的方法 |
CN102657083A (zh) * | 2012-05-04 | 2012-09-12 | 福建农林大学 | 一种铁皮石斛苗茶及其制备方法 |
Non-Patent Citations (1)
Title |
---|
铁皮石斛穴盘育苗基质的优化筛选;孔德栋等;《农业科技通讯》;20101231(第8期);第70页左栏第1.1、1.2节,右栏第1.3节 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106962165A (zh) * | 2017-05-22 | 2017-07-21 | 东莞市睿绅生物技术有限公司 | 一种可移动的铁皮石斛培养基的制作方法及一体化育苗方法 |
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