CN102993321B - 一种黄芪多糖的制备方法 - Google Patents
一种黄芪多糖的制备方法 Download PDFInfo
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- CN102993321B CN102993321B CN201210365416.4A CN201210365416A CN102993321B CN 102993321 B CN102993321 B CN 102993321B CN 201210365416 A CN201210365416 A CN 201210365416A CN 102993321 B CN102993321 B CN 102993321B
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- astragalus polysaccharides
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 33
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 33
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 33
- 241001061264 Astragalus Species 0.000 title claims abstract description 32
- 235000006533 astragalus Nutrition 0.000 title claims abstract description 32
- 210000004233 talus Anatomy 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000000706 filtrate Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000002386 leaching Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 12
- 238000005189 flocculation Methods 0.000 claims abstract description 9
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- 239000012535 impurity Substances 0.000 claims abstract description 7
- 239000002244 precipitate Substances 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 3
- 238000005406 washing Methods 0.000 claims abstract description 3
- 239000006228 supernatant Substances 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 11
- 239000009636 Huang Qi Substances 0.000 claims description 8
- 238000000967 suction filtration Methods 0.000 claims description 5
- 238000004821 distillation Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 10
- 238000010992 reflux Methods 0.000 abstract description 10
- 239000003814 drug Substances 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000000746 purification Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 26
- 239000000284 extract Substances 0.000 description 15
- 239000000463 material Substances 0.000 description 14
- 238000001556 precipitation Methods 0.000 description 12
- 239000000843 powder Substances 0.000 description 11
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 229960004756 ethanol Drugs 0.000 description 4
- 235000018553 tannin Nutrition 0.000 description 4
- 229920001864 tannin Polymers 0.000 description 4
- 239000001648 tannin Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000001471 micro-filtration Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000003252 repetitive effect Effects 0.000 description 3
- 238000005201 scrubbing Methods 0.000 description 3
- 239000001117 sulphuric acid Substances 0.000 description 3
- 108010010803 Gelatin Proteins 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 102000011759 adducin Human genes 0.000 description 2
- 108010076723 adducin Proteins 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
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- 238000005728 strengthening Methods 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- 208000031295 Animal disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- SRBFZHDQGSBBOR-QMKXCQHVSA-N alpha-L-arabinopyranose Chemical compound O[C@H]1CO[C@@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-QMKXCQHVSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002180 anti-stress Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000000496 cardiotonic agent Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000004223 radioprotective effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000005727 virus proliferation Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
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CN201210365416.4A CN102993321B (zh) | 2012-09-27 | 2012-09-27 | 一种黄芪多糖的制备方法 |
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Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103382228A (zh) * | 2013-07-31 | 2013-11-06 | 瑞普(天津)生物药业有限公司 | 一种高活性黄芪多糖的制备方法 |
CN104367987B (zh) * | 2014-12-08 | 2017-07-28 | 河南牧翔动物药业有限公司 | 兽用黄芪制剂及其制备方法 |
CN105995354A (zh) * | 2016-06-23 | 2016-10-12 | 甘肃赫博陇药科技有限责任公司 | 一种增强免疫抗疲劳黄芪多糖功能饮料及其制备工艺 |
CN105906738B (zh) * | 2016-06-29 | 2019-03-22 | 欣格瑞(山东)环境科技有限公司 | 一种鸡冠花中提取分离多糖的方法 |
CN109021131B (zh) * | 2018-07-13 | 2021-04-16 | 仲恺农业工程学院 | 一种用于植物多糖的分离纯化方法 |
CN110423286A (zh) * | 2019-08-30 | 2019-11-08 | 鲁南制药集团股份有限公司 | 一种中药药渣多糖的提取方法及其用途 |
CN112168861A (zh) * | 2020-09-25 | 2021-01-05 | 安徽康信制药股份有限公司 | 一种黄芪颗粒及其制备方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
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US4944946A (en) * | 1987-01-27 | 1990-07-31 | Yaguang Liu | Pharmaceutical composition for inhibiting viruses and increasing immune function (PII) |
IT1238685B (it) * | 1990-02-09 | 1993-09-01 | Indena Spa | Polisaccaridi ad azione immunomodulatrice da astragalus membranaceous e composizioni farmaceutiche che li contengono |
CN1157416C (zh) * | 2001-06-15 | 2004-07-14 | 中国科学院化工冶金研究所 | 絮凝提取海藻多糖的方法 |
CN1295252C (zh) * | 2004-12-08 | 2007-01-17 | 南开大学 | 从黄芪中提取多糖的方法 |
CN100480276C (zh) * | 2007-03-29 | 2009-04-22 | 山东大学 | 一种制备高纯黄芪多糖的方法 |
CN101942035B (zh) * | 2010-09-07 | 2012-03-28 | 天津强微特生物科技有限公司 | 一种根瘤菌胞外多糖的提取及精制方法 |
CN102443072A (zh) * | 2010-10-12 | 2012-05-09 | 沈阳药科大学 | 从黄芪中同时提取多糖和皂苷的方法 |
CN102146142B (zh) * | 2011-03-30 | 2013-07-17 | 泸州品创科技有限公司 | 一种黄芪多糖的制备方法 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of Astragalus Polysaccharide Effective date of registration: 20211224 Granted publication date: 20150603 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd. Registration number: Y2021980016170 |
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Granted publication date: 20150603 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021980016170 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A preparation method of astragalus polysaccharides Granted publication date: 20150603 Pledgee: China Construction Bank Corporation Zhengzhou Railway Sub Branch Pledgor: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd.|HENAN MUXIANG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2024980004821 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
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Address after: No. 9, Yugang Road, Airport Economic Comprehensive Experimental Zone, Zhengzhou City, Henan Province, 451162 Patentee after: Henan Muxiang Biotechnology Co.,Ltd. Country or region after: China Address before: 451162 airport road five, Zhengzhou, Henan Patentee before: HENAN SOAR VETERINARY PHARMACEUTICAL Co.,Ltd. Country or region before: China |