CN102715074A - Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds - Google Patents
Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds Download PDFInfo
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- CN102715074A CN102715074A CN2011103400159A CN201110340015A CN102715074A CN 102715074 A CN102715074 A CN 102715074A CN 2011103400159 A CN2011103400159 A CN 2011103400159A CN 201110340015 A CN201110340015 A CN 201110340015A CN 102715074 A CN102715074 A CN 102715074A
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Abstract
The invention provides a method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds. The method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds mainly comprises selecting an optimal Stevia rebaudiana Bertoni. planting region in China as a Stevia rebaudiana Bertoni. planting base, wherein the optimal Stevia rebaudiana Bertoni. planting region is located at the middle and lower reaches of Changjiang River and in Central and East China, utilizing two varieties of Zhongshan 2 and Zhongshan 3 having excellent quality, good agronomic characters, the basically same flowering phases and good compatibility as Stevia rebaudiana Bertoni. F1 generation seed parents, and carrying out propagation of seed production seedlings of which female parent and male parent amounts are the same by a cuttage asexual propagation method. The method for production of superior F1 generation seeds of Stevia rebaudiana Bertoni. sugar-yielding crops provides a fundamental and effective approach and a guarantee for solving the key problem of Stevia rebaudiana Bertoni. raw material quality improvement in the northern area, in other words for solving the key problem of superior Stevia rebaudiana Bertoni. F1 generation seed sources, provides the maximum possibility for promoting and accelerating the update of the superior Stevia rebaudiana Bertoni. varieties especially in the northern area, and is a technical method used for production of seeds of sugar-yielding commercial crops and having high application and popularization values.
Description
Technical field
The present invention relates to elite seed breeding and the production method of sugar [yielding stevia rebaudianum, belong to plant artificial propagation and cultivation method technical field.
Background technology
Stevia rebaudianum (Stevia rebaudiana Bertoni.) belongs to the composite family herbaceos perennial, self-sow and areas such as the Brazil that is distributed in South America, Paraguay, Argentina.Be known as Paraguay's beet in the original producton location, the Brazilian is the sugar grass.Stevia rebaudianum last century the mid-1970s begin introducing and planting, extensively cultivate in a plurality of countries and regions at present, and the characteristic that stevioside significantly is superior to sucrose with its high sugariness, low in calories etc. becomes the most promising at present a kind of natural health type sugar source gradually.
China is one of country that introduces a fine variety the earliest stevia rebaudianum, and cultivation practices for many years shows that stevia rebaudianum has adaptability widely, all can cultivate in different weather conditions areas such as China northeast, northwest, North China, East China, southwest.Since the own stevia rebaudianum large-scale production, China is maximum in the world stevia rebaudianum primary producing country and exported country all the time, surplus the stevia rebaudianum area under cultivation 40 of China in 2010 ten thousand mu, and south, northern stevia rebaudianum area under cultivation are approximately than respectively accounting for 50% approximately.Because stevia rebaudianum is the perennial plant of cold resistance difference, therefore stevia rebaudianum produces with the main stevia rebaudianum cuttage north of selecting, seedling south and then selects two kinds of different modess of reproduction of seed at present.Because the southern area winter climate is warm relatively; The perennial characteristic of stevia rebaudianum capable of using; Open country or the cold-proof installation open country living plant provenance of preserving stevia rebaudianum improved seeds cottage propagation a little; Generally can come propagation production property seedling through the mode of asexual cuttage in spring, two seasons of autumn, this modes of reproduction can keep the metastable good strains of seeds of kind preferably, postpones deterioration of variety; And northern area is owing to cold weather conditions in winter; The living plant provenance that must solve the good stevia rebaudianum kind cottage propagation of insulation preservation causes the greatly difficult problem of raising of production cost; Therefore generally should not adopt asexual cottage propagation mode, can only select for use seed sexual propagation mode to produce the stevia rebaudianum seedling.The seed of the usefulness of northern area cultivation at present, almost be through for years from originating from numerous accessory seed, and the quality of these accessory seeds degenerates year by year, it has a strong impact on the quality of stevia rebaudianum raw material.Therefore, the seed of the stevia rebaudianum raw material production of northern area use is necessary for high-quality stevia rebaudianum F
1For seed, and this ' good F of stevia rebaudianum
1Kind of technical method ' just solve northern area stevia rebaudianum cured leaf raw material production and use high-quality F
1Crucial seeding technique problem for seed source.
The good F of stevia rebaudianum
1The producing method for seed brief introduction is following:
Selection is possessed of good qualities, well economical character, florescence basically identical, 2 good asexual cottage propagations of stevia rebaudianum kind of compatibility are that plant is to produce good F
1For the parent of seed, through father, the maternal solid good F of stevia rebaudianum of alternate plantation spontaneous pollination that intersects
1For the seed producing method for seed.
Summary of the invention:
The good F of stevia rebaudianum
1Basic demand for the seeding technique method: have that the different target proterties is best in quality, economical character is good, florescence basically identical, 2 good kinds of compatibility; Two stevia rebaudianum kinds are bred clone plant seedling through the equivalent that needs that cuttage is produced by every mu 10,000 strain respectively, as producing good F
1The maternal seedling in generation; Every kind plant must carry out adjacent spaces field planting cultivation with the behavior base unit; Different flower between every adjacent lines stevia rebaudianum kind individual plant; Can accomplish pollination sexual hybridization process (because the genetic background between the individuality of each kind vegetative propagation plant is in full accord, the compatibility of selfing is almost nil in its kind) naturally through entomophila respectively; The F that is produced through this combo mode of planting
1Generally have fine quality that parents have and good economical character for the seed progeny, to guarantee to use this F
1Stevia rebaudianum cured leaf raw material for seed production has good quality.
The good F of stevia rebaudianum
1The specific requirement of producing method for seed is following:
1, production of hybrid seeds base is selected:
Though stevia rebaudianum all can be cultivated in China different weather conditions area as raw material production; But because stevia rebaudianum is a short-day plant; Generally late blooming can occur and the phenomenon of early flowering occur, so these two areas are all suitable selects as production of hybrid seeds base at low latitudes in the high latitude area cultivation.The best region of selecting in China stevia rebaudianum production of hybrid seeds base is mainly the provinces and regions, the middle and lower reach of Yangtze River in east and central China area.
2, the good F of stevia rebaudianum
1Producing method for seed parent's selection:
It is high R-A steviol glycoside content (R-A glycosides/total glycosides is more than 70%) that parent's quality trait requires one of parent, two be the total glycosides of high stevia rebaudianum (always glycosides/cured leaf content is more than 13%); Two parent's output are all more than 200kg/ mu; The florescence basically identical requires the interracial early flowering season to be no more than 10 days; Two parent's compatibilities are good, and the cross-fertile rate under the natural situation is not less than 30%; The cottage propagation rate is high, and year reproduction coefficient of production of hybrid seeds parental breed is more than 50; Two parents all have good disease resistance.The good F that selects for use above two clone group gas-mixing hybridizations to obtain
1For the stevia rebaudianum seed, the R-A glycosides of the cured leaf material quality of being produced/total glycosides is more than 55%, and total glycosides/cured leaf content is more than 13%.
3, whole ground bedding
Select the subsequent use cultivated land dry land of non-continuous cropping, impose an amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.The area in annual 1 season of plantation is put in order ground and is accomplished 10～November before going into the winter, and the area of planting for two seasons requires to accomplish in preceding 30 days in the field planting of kind plant then 3～April wholely.With the bedding of common farmland is wide 80cm and high 10cm, furrow spacing 40cm.
4, field planting and cultivation management
The good F of stevia rebaudianum
1The setting date of producing seedling for seed is in 4～May, field planting seedling plant height 10～15cm, and 3 pairs of tools are with blade.Plantation two row on the wide furrow of 80cm (father, maternal each 1 row), respectively apart from furrow edge 20cm, line space is 40cm, spacing in the rows is 20cm; Father in the growing area, maternally carry out the adjacent spaces plantation with behavior unit, every furrow father, female parent are planted 1 row, must guarantee with about two furrow neighbours intersect plantation for different cultivars in the ranks.Water permeablely after the field planting, multiple permeable 1～2 time of next day, undertaken by the living crop water of general drought, fertilizer and field management such as hoe up weeds behind the seedling-slowing stage.
5, stevia rebaudianum bloom, solid and seed results
Stevia rebaudianum about September～October, between two kind plant of the mutual plantation of production of hybrid seeds milpa, is accomplished this sexual hybridization process of pollination through entomophila respectively at the full-bloom stage in the middle and lower reach of Yangtze River area naturally, is 30～40 days certainly the seed maturity that is pollinated; Seed is since early October maturation successively, and the seed results select at 10 in the fine morning to carry out in the past at 17 in afternoon.The good F of stevia rebaudianum
130～40%, the germination rate of seed is 20～30% for the natural ripening rate of the production of hybrid seeds, 15～20 jin, a traditional unit of weight of seed per mu yields.
6, the cleaning of seed
The seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and impurity respectively keeping away cool solarization the under the direct light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
Embodiment:
Embodiment 1
The good F of stevia rebaudianum
1Producing method for seed: production of hybrid seeds base is chosen in the suburbs, Nanjing, Jiangsu Province of the middle and lower reach of Yangtze River; Select the good stevia rebaudianum kind of parent ' No. 2, middle mountain ' and ' No. 3, middle mountain ', ' No. 2, middle mountain ' is high stevia rebaudianum R-A glycosides type kind (R-A glycosides/total glycosides is 79%), and ' No. 3, middle mountain ' is the total glycosides type of high stevia rebaudianum kind (total glycosides/cured leaf content 13.9%).Adopt cuttage vegetative propagation mode crossbreeding to produce respectively to two kinds with each 1,000,000 strain of seedling, as 100 mu of good F of stevia rebaudianum
1Hybrid strain seedling for the seed production base.
1, whole ground bedding: select 100 mu of subsequent use cultivated land dry lands, impose an amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.With the direct bedding in common farmland is wide 80cm and high 10cm, furrow spacing 40cm.
2, field planting and field management: more than 3～5 pairs of blade plant heights of field planting seedling, 10～15cm, field planting on April 20～25, plantation 2 row on the wide furrow of every 80cm, respectively apart from furrow edge 20cm, line-spacing is 40cm, spacing in the rows is 20cm; ' No. 2, middle mountain ' and ' No. 3, middle mountain ' every kind of every furrow plant 1 the row, about two furrow close on capable alternate the planting of not repeating.Water permeablely after the field planting, multiple permeable 1～2 time of next day, slow seedling 5～7 days waters permeable 1 time according to weather rainwater situation 5～7 afterwards, imposes composite fertilizer between seedling 1 time in 40～50 days after the field planting, in time hoes up weeds, loosens the soil, field management such as the extermination of disease and insect pest.
3, results seed: ' No. 2, middle mountain ' and ' No. 3, middle mountain ' full-bloom stage is about 9～October, and seed is maturation successively after the early October generally.The seed results select sunny weather to carry out.The seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and impurity respectively keeping away cool solarization the under the direct light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
4, seed production and quality: the good F of stevia rebaudianum
135%, the germination rate of seed is 25% for the natural ripening rate of the production of hybrid seeds, 18 jin, a traditional unit of weight of seed per mu yields.The good F of stevia rebaudianum
1For the seed variety quality characteristic is that total glycosides content is 14-15%, and R-A glycosides/total glycosides is 56-57%.
Claims (7)
1. the good F of stevia rebaudianum
1Producing method for seed is characterized in that the good F of stevia rebaudianum
1It is that female parent ' No. 3, middle mountain ' is a male parent that production of hybrid seeds parent requires with ' No. 2, middle mountain ', or is that female parent ' No. 2, middle mountain ' is a male parent with ' No. 3, middle mountain '; Production of hybrid seeds milpa is selected the cultivated land dry land at nearly water source; Two stevia rebaudianum kinds are respectively through cottage propagation clone plant seedling, as producing good F
1The maternal seedling in generation; Setting date is 4～May, production of hybrid seeds parent must guarantee to hold same furrow father, female parent respectively do for oneself row and with about two furrow close on the alternate field planting of capable parent; About 9～October, seed begins the maturing stage in early October to stevia rebaudianum at the full-bloom stage in middle and lower reach of Yangtze River area; The seed results select sunny weather to carry out, and the seed of results is being kept away cold solarization cleaning back dress sealed bag preservation under the direct light.
2. the good F of stevia rebaudianum according to claim 1
1Producing method for seed is characterized in that the best region that China stevia rebaudianum production of hybrid seeds base is selected is mainly the regional provinces and regions, the middle and lower reach of Yangtze River of east and central China.
3. the good F of stevia rebaudianum according to claim 1
1Producing method for seed is characterized in that the good F of stevia rebaudianum
1For production of hybrid seeds parent be possessed of good qualities, good economical character, florescence basically identical, good ' No. 2, middle mountain ' and ' No. 3, the middle mountain ' kind of compatibility, adopt cuttage vegetative propagation mode that the seedling of two quantity such as variety breeding is used for production of hybrid seeds production respectively.
4. the good F of stevia rebaudianum according to claim 1
1Producing method for seed is characterized in that need accomplishing in preceding 30 days in field planting of production of hybrid seeds milpa wholely, imposes N, P, every mu of 50Kg of K composite fertilizer as base manure before the whole ground; Whole ground is with the wide 80cm of the direct bedding in common farmland; High 10cm, furrow spacing 40cm, furrow length is confirmed according to planting site length.
5. the good F of stevia rebaudianum according to claim 1
1Producing method for seed is characterized in that setting date is 4～May, field planting seedling plant height 10～15cm; And 3 pairs of tools are with blade; Two parent's field planting must be guaranteed this father, maternal in the wide same furrow of 80cm, respectively planted delegation, and respectively apart from furrow edge 20cm, line-spacing is 40cm; Spacing in the rows is 20cm, and guarantee and about two furrow close on this alternate planting of capable father, mother.
6. the good F of stevia rebaudianum according to claim 1
1Producing method for seed is characterized in that the stevia rebaudianum full-bloom stage in September～October, accomplishes pollination sexual hybridization process naturally through entomophila between two kind plant, and seed is since early October maturation successively, selects at 10 in fine day morning to carry out seed in the past at 17 in afternoon and gathers in the crops.
7. the good F of stevia rebaudianum according to claim 1
1Producing method for seed, the seed that it is characterized in that gathering in the crops is being kept away cool solarization the under the direct light, and the seed dress sealed bag of using thick, the dusting cover of 0.5 * 0.5cm and 0.15 * 0.15cm to remove after branches and leaves and the impurity cleaning is respectively preserved the good F of stevia rebaudianum
1For the seed variety quality characteristic is that total glycosides content is 14-15%, and R-A glycosides/total glycosides is 56-57%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885929A (en) * | 2015-06-18 | 2015-09-09 | 云南农业大学 | Hybrid seed production method for Erigeron breviscapus (Vant.) Hand.-Mazz. |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN105104169A (en) * | 2015-08-23 | 2015-12-02 | 林平 | Mating method for stevia rebaudiana hybrid seed production |
| CN112119910A (en) * | 2020-10-30 | 2020-12-25 | 海南瑞民农业科技有限公司 | Melon and fruit pollination method |
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| CN1327720A (en) * | 2001-07-11 | 2001-12-26 | 青岛创升生物科技有限公司 | Method for breeding hybridized seed of stevia rebaudinum |
| CN101213934A (en) * | 2008-01-08 | 2008-07-09 | 江苏省中国科学院植物研究所 | Single clone choiceness F1 stevia rebaudianum hybrid seeds production method |
| CN101213935A (en) * | 2008-01-08 | 2008-07-09 | 江苏省中国科学院植物研究所 | Double clone choiceness F1 stevia rebaudianum hybrid seeds production method |
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2011
- 2011-11-01 CN CN2011103400159A patent/CN102715074A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327720A (en) * | 2001-07-11 | 2001-12-26 | 青岛创升生物科技有限公司 | Method for breeding hybridized seed of stevia rebaudinum |
| CN101213934A (en) * | 2008-01-08 | 2008-07-09 | 江苏省中国科学院植物研究所 | Single clone choiceness F1 stevia rebaudianum hybrid seeds production method |
| CN101213935A (en) * | 2008-01-08 | 2008-07-09 | 江苏省中国科学院植物研究所 | Double clone choiceness F1 stevia rebaudianum hybrid seeds production method |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104885929A (en) * | 2015-06-18 | 2015-09-09 | 云南农业大学 | Hybrid seed production method for Erigeron breviscapus (Vant.) Hand.-Mazz. |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN105104169A (en) * | 2015-08-23 | 2015-12-02 | 林平 | Mating method for stevia rebaudiana hybrid seed production |
| CN112119910A (en) * | 2020-10-30 | 2020-12-25 | 海南瑞民农业科技有限公司 | Melon and fruit pollination method |
| CN112119910B (en) * | 2020-10-30 | 2021-11-02 | 海南瑞民农业科技有限公司 | Wax gourd pollination method |
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Application publication date: 20121010 |