CN105104169A - Mating method for stevia rebaudiana hybrid seed production - Google Patents

Abstract

The invention discloses a mating method for stevia rebaudiana hybrid seed production. The mating method includes the steps that the parent single plant of stevia rebaudiana is subjected to tissue culture and propagation, parent plant systems which are consistent in genotype and reproduction feature are formed, the two different parent plant systems are planted alternately in rows in the same field to conduct mutual natural hybridization, and stevia rebaudiana hybrid seeds are obtained. The mating method overcomes the defects that stevia rebaudiana seed seedling groups cannot conduct directional hybridization, group separation is large and characters are instable, and good stevia rebaudiana hybrid seed combinations which are relatively stable in character can be provided.

Description

A kind of matching method of stevia hybrid seeding

Technical field

the present invention relates to the seeding technique of stevia crossbreed, is a kind of matching method of stevia hybrid seeding in particular.

Background technology

the kind that current stevia uses is all the conventional variety adopting systematic breeding or filial generation isolation and selection to go out, and the technical merit of breed breeding is lower, seriously limits the development that stevia is produced.According to the genetic development of crops, adopt cross-breeding technology, utilize the hybrid vigour of a F1 generation can the advantage of comprehensive different stevia parent, increase substantially economic flow rate and the quality of stevia, bring new growth engines to seed industry simultaneously.

according to the method for general crops cross breeding seed, be by the seed sowing of Parent or seedling raising and transplanting in one piece of farmland, carry out mutual hybrid seeding, the hybrid seed producing F1 generation for the production of.If but the stevia seed seedling of Liang Ge colony carries out natural hybrization in field, not only have the phase mutual cross between two colonies, also have the hybridization between the inner plant of colony, the population characteristics of its offspring is by disorderly and unsystematic, predictable stable character cannot be formed, also cannot be applied to production.

Summary of the invention

the object of the invention is to produce Problems existing for above-mentioned stevia crossbreed, propose a kind of solution of new stevia hybrid seeding.

the present invention is achieved in that

a matching method for stevia hybrid seeding, comprises the steps:

(1) parent's strain tissue culture expanding propagation: in group training room, tissue culture expanding propagation is carried out to the individual plant of the parent of stevia, form the production of hybrid seeds parent strain that genotype is consistent with reproductive characteristic;

(2) hybrid seeding: respectively by 2 different stevia parent strains by row alternate field planting in same field, carry out mutual natural hybrization, obtain stevia crossbreed.

beneficial effect of the present invention:

this case proposes with parent's strain of the mode crossbreeding of individual plant group training, plant all in a colony is all from same plant, it is a vegetative colony, each plant in colony is attending to anything else of former maternal plant, genotype in colony between individuality is just the same, and reproductive characteristic can be considered an individual plant.Because stevia has the acarpous characteristic of selfing, so not only intragroup individual selfing can not be solid, between each individuality can not mating solid.Be planted in together by two different parent's strains, can not carry out mating solid between the flower of each colony inside, the plant between colony then can cross-fertile freely mutually, defines directed hybridization.The crossbreed of producing like this can keep the relatively stable of kind of property, the excellent proterties that different parent has can be combined by such hybridization, improves the output of F1 generation, quality and resistance, realizes high yield, stable yields and quality product matter.

Embodiment

a matching method for stevia hybrid seeding, comprises the steps:

(1) parent's strain tissue culture expanding propagation: in group training room, tissue culture expanding propagation is carried out to the individual plant of the parent of stevia, form the production of hybrid seeds parent strain that genotype is consistent with reproductive characteristic;

(2) hybrid seeding: respectively by 2 different stevia parent strains by row alternate field planting in same field, carry out mutual natural hybrization, obtain stevia crossbreed.

Claims (1)

1. a matching method for stevia hybrid seeding, is characterized in that, it comprises the steps:

(1) parent's strain tissue culture expanding propagation: in group training room, tissue culture expanding propagation is carried out to the individual plant of the parent of stevia, form the production of hybrid seeds parent strain that genotype is consistent with reproductive characteristic;

(2) hybrid seeding: respectively by 2 different stevia parent strains by row alternate field planting in same field, carry out mutual natural hybrization, obtain stevia crossbreed.