CN1653884A - Method for culturing sweet pepper cenospecies by genic male sterility dual purpose lines - Google Patents
Method for culturing sweet pepper cenospecies by genic male sterility dual purpose lines Download PDFInfo
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- CN1653884A CN1653884A CN 200510012378 CN200510012378A CN1653884A CN 1653884 A CN1653884 A CN 1653884A CN 200510012378 CN200510012378 CN 200510012378 CN 200510012378 A CN200510012378 A CN 200510012378A CN 1653884 A CN1653884 A CN 1653884A
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Abstract
The method of breeding sweet pepper hybrid with nuclear two-purpose male sterility line of sweet pepper features the breeding process, which includes the cross-breeding, selfing and sib breeding process to transfer the male sterile nuclear gene (msms) into selfing sweet pepper line with excellent agricultural character to obtain nuclear two-purpose male sterility line of sweet pepper of sterile plant rate of 50 %; the agricultural character selectivity of sib breeding for 2 or 3 generations to selectively breed the two-purpose male sterility line of sweet pepper with stable agricultural character, stable sterile plant rate of about 50 % and sterile degree of 100 %; culturing the hybrid seed with the sterile plants of the nuclear two-purpose male sterility line as female parent and the selfing line as male parent, removing the breeding plants before pollination and collecting seeds of the female parent. The present invention uses the nuclear male sterility line as both sterile line and maintainer line to simplify the breeding process.
Description
Technical field
What the present invention relates to is a kind of breeding method of variety of crops, specifically a kind of method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed.
Background technology
Pimento hybrid vigour is remarkable, and therefore cultivating crossbreed is an effective way that improves output, improvement quality, strengthens resistance.Present pimento method for culturing cenospecies: 1, utilize hybridization between selfed lines seed selection crossbreed.When adopting this method hybrid seeding, need flower bud phase artificial emasculation, because the pimento floral organ is little and tender and crisp, flower bud phase artificial emasculation operation easier is big, requires technical by force, and recruitment is many, cost is high, and purity is difficult to assurance.2, patent 02125723.X discloses a kind of method of utilizing the capsicum or the production of hybrid seeds of pimento cytoplasm male sterility line, though this method has overcome the shortcoming of above-mentioned inbred line breeding crossbreed to a certain extent, but because the male sterility of the cytoplasmic sterile line that it utilizes is controlled by cell nucleus and plasmon, utilize cytoplasmic sterile line to cultivate crossbreed, not only need select male sterile line, also need select maintenance line, select and recover system and reach three series mating again, could cultivate into crossbreed, the procedure of breeding is loaded down with trivial details, and the time is long; Because the pimento cytoplasmic sterile line lacks the recovery source, it is bigger that the recovery that selects system cultivates into the crossbreed difficulty, and " three are " self breeding save routine is also loaded down with trivial details.
Summary of the invention
To the objective of the invention is the defective that exists in the above-mentioned prior art in order overcoming, a kind of method of utilizing the pimento genic male sterility dual purpose to cultivate the pimento crossbreed to be provided.The genic male sterility dual purpose one that utilizes in this method be dual-purpose promptly be that male sterile line is again a maintenance line, and breed simultaneously, thereby simplify the procedure of breeding, can cultivate the significant crossbreed of hybrid vigour quickly.
The object of the present invention is achieved like this: this method of utilizing genic male sterility dual purpose to cultivate the pimento crossbreed, it is characterized in that adopting following steps to finish: A, the method for utilizing hybridization, selfing, sisters to hand over, male sterile nuclear gene (msms) transformation in the good pimento inbred line of economical character, is obtained sterile plant rate and is 50% pimento genic male sterility dual purpose;
B, hand over sterile dual-purpose objective trait to carry out seed selection, obtain that economical character is good, sterile plant rate is stabilized in 50% pimento genic male sterility dual purpose steps A by sisters;
C, with sterile strain in the step B system be female parent, can to educate strain be that male parent is carried out sisters and handed over this male sterile two-purpose line seed of breeding, according to the sterile strain of marker trait mark that can educate strain and sterile strain floral organ, pull out after the end of pollinating and can educate strain before the pollination;
D, being female parent with the sterile strain in the step C genic male sterility dual purpose, is that male parent is cultivated crossbreed with selfing, in time pulls out all before the pollination and can educate strain, and the maternal seed of going up of gathering can obtain the crossbreed of 100% purity.
The described method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed, pimento kernel male sterile gene source is in the pimento genic male sterility dual purpose AB of Hebei Academy of Agriculture and Forestry Science, Economic Crops Institute's seed selection
91
The described method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed, the propagation method of this male sterile two-purpose line seed is among steps A-C:
Seed treatment: earlier this male sterile two-purpose line seed is carried out disinfection: adopt 55 ℃ after water seed soaking 20-30 minute, with 1% copper sulphate seed soaking 5 minutes, clean with flushing with clean water then, again with 10% tertiary sodium phosphate seed soaking 15 minutes, clean with flushing with clean water, soaked seed at normal temperatures 12-14 hour, and pulled drying moisture out and put 26-28 ℃ of vernalization, the back sowing of sprouting;
Sowing field planting: in 12 months, adopt greenhouse by solar heat sowing the last ten-days period, broadcast the 80-90 gram for every mu, seedling age 75-90 days, when seedling age 8-9 sheet true leaf, plant are colonizated in the plastics big arch shed during up to 18-22cm, distance isolation and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field or the isolation of employing grenadine, field planting distance between rows and hills 50cm * 20cm, the individual plant cultivation, mu seedling 6600 strains; Use sufficient base manure, irrigate field planting water, watered one time water behind the slow seedling in 5-7 days, watered one time water in later per 7～10 days; The 1-2 layer the flowers are in blossom put during, differentiate the sterile strain of mark according to the marker trait that can educate strain and sterile strain floral organ; Powder is adopted in 1/3 the educated strain that meets this male sterile line feature of selecting and remain, and gives sterile strain pollination, pulls out all the other and can educate strain; Pollination is in time pulled out all and can be educated strain after finishing.
The seed of gathering: 50～60 days fruits in pollination back change red when ripe, kind of the fruit of gathering in sterile strain; Fruit left by plane, takes out seed, airing at once, and the moisture time shift of drying the surface of the seed is to the natural seasoning of shady and cool ventilation; Seed is placed on the storage down of low temperature, drying condition.
The described method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed, when crossbreed bred, above-mentioned genic male sterility dual purpose cultivated area ratio maternal and the inbred line male parent was 4: 1.
The described method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed, above-mentioned sterile strain with the marker trait that can educate the strain floral organ is: sterile strain flower is long stigma, pale reddish brown medicine, the flower pesticide slight of stature is shrivelled, cannot see pollen after not ftractureing or ftractureing; Can educate the strain flower and be the short column head, the chrysanthemum medicine, the full hypertrophy of flower pesticide is covered with pollen behind the cracking.
The described method of utilizing the pimento genic male sterile line to cultivate crossbreed, the method for cultivating crossbreed described in the step D is:
With genic male sterility dual purpose AB
91-S909-3-2Be female parent, pimento inbred line ZH4 is a male parent, and this plants father and mother by 1: 4 area ratio, adopts little high plot of land film mulching and planting, every ridge cultivation two row, wide row space 60cm, little line-spacing 40cm, maternal individual plant is planted, spacing in the rows 14cm, the male parent cave is apart from 30cm-33cm, the two strains in every cave, father and mother originally concentrate field planting respectively;
Seed farm and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field; In the maternal colony 50% sterile strain is arranged, 50% can educate strain, the 1-2 layer the flowers are in blossom put during, the marker trait according to educating strain and sterile strain floral organ pulls out only and can educate strain, stays sterile strain and pollinates; Gather the suitable bud that male parent can open in second day, take out flower pesticide and dry at the shady and cool ventilation place, the next morning sieve get pollen, put into the pollination pipe, select the flower that opened the same day in the sterile strain, pollinate; The pollination preference temperature, 20～25 ℃ of daytimes, 15～18 ℃ of nights, in the middle of one day, during with 6-11 and during 16-20 for well.
Advantage of the present invention is: 1, utilize pimento genic male sterility dual purpose hybrid seeding, province's phase artificial emasculation, allocarpy mark, pollination many links such as plant arrangement after finishing that disbud, simplified the hybrid seeding program, greatly reduce hybrid seeding difficulty and cost, save recruitment 2/3, mu reduces cost about 50%.Because its gynoecium is grown normal, ripening rate, the rate of setting seeds are higher, economize the phase artificial emasculation that disbuds again, can avoid the mechanical damage column cap, improve ripening rate again, the rate of setting seeds, 28.4 kilograms of average yield per mu kind amounts, the highest mu seed yield can reach 42.6 kilograms, avoid that loose powder forms pseudostationary because of castrating in clean or the flower bud in advance, can guarantee hybrid seed purity, the purity of hybrid that obtains in sterile strain can reach 100%.2, the male sterility of pimento genic male sterility dual purpose is controlled by a pair of recessive nuclear gene, heredity is simple, its seed selection and transfer method are easier, can be according to objective trait, and transformation in the short period of time becomes the serial genic male sterility dual purpose that economical character is good, coordinate force is high.This genic male sterility dual purpose promptly can be gone bail for, and to hold be that the breeding that is used for self is preserved, can pull out again and can educate strain and do male sterile line and hybridization between selfed lines, be used for breeding hybridized and seed production, one be dual-purpose promptly be that male sterile line is again a maintenance line, and breed simultaneously, lack a seed selection of overlapping maintenance line than cytoplasmic sterile line (three line method), simplified the procedure of breeding, shortened breeding time, and sterility inheritance stability, sterile degree 100% is not subject to environmental influence, and the recovery source is extensive, general kind and inbred line all are its recovery systems, the assembly degree of freedom is big, and easily selecting good recovery is to cultivate the significant crossbreed of hybrid vigour quickly.
Embodiment:
1, the transformation of pimento genic male sterility dual purpose sterile gene
With the genic male sterility dual purpose AB of Hebei Academy of Agriculture and Forestry Science, Economic Crops Institute
91Be sterile source, it is shaped as red side's bird-caging, with redness side bird-caging inbred line S909 is transformation target parent, method through hybridization, selfing, sisters' friendship, with kernel male sterile gene (msms) transformation in S909 pimento inbred line, hand over by 2 generation sisters simultaneously and carry out the selection of economical character, select that economical character is stable, sterile plant rate is stabilized in about 50%, the pimento male sterile two-purpose line AB of sterile degree 100%
91-S909-3-2
2, the breeding of this genic male sterility dual purpose seed
Earlier this genic male sterility dual purpose seed is carried out disinfection: adopt 55 ℃ after water seed soaking 20-30 minute, with 1% copper sulphate seed soaking 5 minutes, clean with flushing with clean water then, again with 10% tertiary sodium phosphate seed soaking 15 minutes, clean with flushing with clean water, soaked seed at normal temperatures 12-14 hour, and pulled drying moisture out and put 26-28 ℃ of vernalization, the back sowing of sprouting.
Sowing field planting: in 12 months, adopt greenhouse by solar heat sowing the last ten-days period, broadcast the 80-90 gram for every mu, seedling age 75-90 days, when seedling age 8-9 sheet true leaf, plant are colonizated in the plastics big arch shed during up to the 20cm left and right sides, distance isolation and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field or the isolation of employing grenadine, field planting distance between rows and hills 50cm * 20cm, the individual plant cultivation, mu seedling 6600 strains.Use sufficient base manure, irrigate field planting water, watered one time water behind the slow seedling in 5-7 days, watered one time water in later per 7～10 days.The 1-2 layer the flowers are in blossom put during, differentiate the sterile strain of mark according to the marker trait that can educate strain and sterile strain floral organ.Powder is adopted in 1/3 the educated strain that meets this male sterile line feature of selecting and remain, and gives sterile strain pollination, pulls out all the other and can educate strain.Pollination is in time pulled out all and can be educated strain after finishing.
The seed of gathering: 50～60 days fruits in pollination back change red when ripe, kind of the fruit of gathering in sterile strain.Fruit left by plane, takes out seed, airing at once, and the moisture time shift of drying the surface of the seed is to the natural seasoning of shady and cool ventilation; Seed is placed on the storage down of low temperature, drying condition.
4, utilize this genic male sterility dual purpose to cultivate the pimento hybrid seed
With AB
91-S909-3-2Be female parent, with 12 pimento selfings is the combination of male parent preparing hybrid, and this plants father and mother by 1: 4 area ratio, adopts little high plot of land film mulching and planting, every ridge cultivation two row, wide row space 60Cm, little line-spacing 40Cm, maternal individual plant is planted, spacing in the rows 14Cm, the male parent cave is apart from 30-33Cm, the two strains in every cave, and father and mother originally concentrate field planting respectively.Seed farm and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field.In the maternal colony 50% sterile strain is arranged, 50% can educate strain, the 1-2 layer the flowers are in blossom put during, the marker trait according to educating strain and sterile strain floral organ pulls out only and can educate strain, stays sterile strain and pollinates.Gather the suitable bud that male parent can open in second day, take out flower pesticide and dry at the shady and cool ventilation place, the next morning sieve get pollen, put into the pollination pipe, select the flower that opened the same day in the sterile strain, pollinate.The pollination preference temperature, 20～25 ℃ of daytimes, 15～18 ℃ of nights, in the middle of one day, during with 6-11 and during 16-20 for well.The hybrid seed Seed Collecting Methods is with the collecting method of male sterile two-purpose line seed.
Measure through continuous 2 years field evaluation, disease resistance evaluation, Analysis of Combining Ability, therefrom select specific combining ability height, economical character performance excellence and the good " AB of commodity
91-S909-3-2* ZH4 " combination, through kind comparison and regional test, cultivate into pimento male sterile series hybrid " grind No. 12 Ji ".
Claims (6)
1, a kind of method of utilizing the pimento genic male sterility dual purpose to cultivate crossbreed is characterized in that adopting following steps to finish:
A, the method for utilizing hybridization, selfing, sisters to hand in the good pimento inbred line of economical character, obtain sterile plant rate and are 50% pimento genic male sterility dual purpose with the transformation of male sterile nuclear gene;
B, hand over sterile dual-purpose objective trait to carry out seed selection, obtain that economical character is good, sterile plant rate is stabilized in 50% pimento genic male sterility dual purpose steps A by sisters;
C, with sterile strain in the step B system be female parent, can to educate strain be that male parent is carried out sisters and handed over this male sterile two-purpose line seed of breeding, according to the sterile strain of marker trait mark that can educate strain and sterile strain floral organ, pull out after the end of pollinating and can educate strain before the pollination;
D, being female parent with the sterile strain in the step C genic male sterility dual purpose, is that male parent is cultivated crossbreed with selfing, in time pulls out all before the pollination and can educate strain, and the maternal seed of going up of gathering can obtain the crossbreed of 100% purity.
2, the method for utilizing the pimento genic male sterility dual purpose to cultivate crossbreed according to claim 1 is characterized in that the pimento genic male sterility dual purpose AB of pimento kernel male sterile gene source in Hebei Academy of Agriculture and Forestry Science, Economic Crops Institute's seed selection
91
3, the method for utilizing the pimento genic male sterility dual purpose to cultivate crossbreed according to claim 1 is characterized in that the propagation method of this male sterile two-purpose line seed among steps A-C is:
Seed treatment: earlier this male sterile two-purpose line seed is carried out disinfection: adopt 55 ℃ after water seed soaking 20-30 minute, with 1% copper sulphate seed soaking 5 minutes, clean with flushing with clean water then, again with 10% tertiary sodium phosphate seed soaking 15 minutes, clean with flushing with clean water, soaked seed at normal temperatures 12-14 hour, and pulled drying moisture out and put 26-28 ℃ of vernalization, the back sowing of sprouting;
Sowing field planting: in 12 months, adopt greenhouse by solar heat sowing the last ten-days period, broadcast the 80-90 gram for every mu, seedling age 75-90 days, when seedling age 8-9 sheet true leaf, plant are colonizated in the plastics big arch shed during up to 18-22cm, distance isolation and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field or the isolation of employing grenadine, field planting distance between rows and hills 50cm * 20cm, the individual plant cultivation, mu seedling 6600 strains; Use sufficient base manure, irrigate field planting water, watered one time water behind the slow seedling in 5-7 days, watered one time water in later per 7～10 days; The 1-2 layer the flowers are in blossom put during, differentiate the sterile strain of mark according to the marker trait that can educate strain and sterile strain floral organ; Powder is adopted in 1/3 the educated strain that meets this male sterile line feature of selecting and remain, and gives sterile strain pollination, pulls out all the other and can educate strain; Pollination is in time pulled out all and can be educated strain after finishing.
The seed of gathering: 50～60 days fruits in pollination back change red when ripe, kind of the fruit of gathering in sterile strain; Fruit left by plane, takes out seed, airing at once, and the moisture time shift of drying the surface of the seed is to the natural seasoning of shady and cool ventilation; Seed is placed on the storage down of low temperature, drying condition.
4, the method for utilizing the pimento genic male sterility dual purpose to cultivate crossbreed according to claim 1 is characterized in that when crossbreed breeds, and above-mentioned genic male sterile line female parent and inbred line male parent cultivated area ratio are 4: 1.
5, the method for utilizing the pimento genic male sterility dual purpose to cultivate crossbreed according to claim 1, it is characterized in that above-mentioned sterile strain and the marker trait that can educate the strain floral organ are: sterile strain flower is long stigma, pale reddish brown medicine, the flower pesticide slight of stature is shrivelled, not cannot see pollen after not ftractureing or ftractureing; Can educate the strain flower and be the short column head, the chrysanthemum medicine, the full hypertrophy of flower pesticide is covered with pollen behind the cracking.
6, the method for utilizing the pimento genic male sterility dual purpose to cultivate crossbreed according to claim 1 is characterized in that the method for cultivating crossbreed described in the step D is:
With genic male sterile line AB
91-S909-3-2Be female parent, pimento inbred line ZH4 is a male parent, and this plants father and mother by 1: 4 area ratio, adopts little high plot of land film mulching and planting, every ridge cultivation two row, wide row space 60cm, little line-spacing 40cm, maternal individual plant is planted, spacing in the rows 14cm, the male parent cave is apart from 30cm-33cm, the two strains in every cave, father and mother originally concentrate field planting respectively;
Seed farm and other pimento product till the land and isolate at least more than the 500m, isolate more than the 1000m at least with other capsicum variety field; In the maternal colony 50% sterile strain is arranged, 50% can educate strain, the 1-2 layer the flowers are in blossom put during, the marker trait according to educating strain and sterile strain floral organ pulls out only and can educate strain, stays sterile strain and pollinates; Gather the suitable bud that male parent can open in second day, take out flower pesticide and dry at the shady and cool ventilation place, the next morning sieve get pollen, put into the pollination pipe, select the flower that opened the same day in the sterile strain, pollinate; The pollination preference temperature, 20～25 ℃ of daytimes, 15～18 ℃ of nights, in the middle of one day, during with 6-11 and during 16-20 for well.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100382672C (en) * | 2005-11-24 | 2008-04-23 | 四川大学 | Large piment secondary cultivating method |
| CN105766623A (en) * | 2016-04-07 | 2016-07-20 | 青岛金妈妈农业科技有限公司 | Production method for pepper hybrids |
| CN107787827A (en) * | 2016-08-31 | 2018-03-13 | 辽宁东亚农业发展有限公司 | A kind of spicy green pepper new variety selective breeding technology of precocious lower temperature resistance |
| CN107912294A (en) * | 2017-11-08 | 2018-04-17 | 河北省农林科学院经济作物研究所 | A kind of selection of fruit type yellow pimento |
| CN108739327A (en) * | 2018-06-19 | 2018-11-06 | 安徽省农业科学院园艺研究所 | A kind of chilli kernel male sterile dual purpose lines producing method for seed |
-
2005
- 2005-03-11 CN CN 200510012378 patent/CN1653884A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100382672C (en) * | 2005-11-24 | 2008-04-23 | 四川大学 | Large piment secondary cultivating method |
| CN105766623A (en) * | 2016-04-07 | 2016-07-20 | 青岛金妈妈农业科技有限公司 | Production method for pepper hybrids |
| CN105766623B (en) * | 2016-04-07 | 2018-05-11 | 青岛金妈妈农业科技有限公司 | A kind of producing method for seed of capsicum cenospecies |
| CN107787827A (en) * | 2016-08-31 | 2018-03-13 | 辽宁东亚农业发展有限公司 | A kind of spicy green pepper new variety selective breeding technology of precocious lower temperature resistance |
| CN107912294A (en) * | 2017-11-08 | 2018-04-17 | 河北省农林科学院经济作物研究所 | A kind of selection of fruit type yellow pimento |
| CN107912294B (en) * | 2017-11-08 | 2020-10-30 | 河北省农林科学院经济作物研究所 | Breeding method of fruit type yellow sweet peppers |
| CN108739327A (en) * | 2018-06-19 | 2018-11-06 | 安徽省农业科学院园艺研究所 | A kind of chilli kernel male sterile dual purpose lines producing method for seed |
| CN108739327B (en) * | 2018-06-19 | 2020-05-29 | 安徽省农业科学院园艺研究所 | Seed production method of capsicum annuum nuclear male sterile dual-purpose line |
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