CN101932229B - Plant capable of bearing seedless fruits and method of producing variety bearing seedless fruits - Google Patents
Plant capable of bearing seedless fruits and method of producing variety bearing seedless fruits Download PDFInfo
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- CN101932229B CN101932229B CN2009801039701A CN200980103970A CN101932229B CN 101932229 B CN101932229 B CN 101932229B CN 2009801039701 A CN2009801039701 A CN 2009801039701A CN 200980103970 A CN200980103970 A CN 200980103970A CN 101932229 B CN101932229 B CN 101932229B
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- parthenocarpy
- individuality
- male sterility
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- capsicum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/08—Fruits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
- A01H1/023—Male sterility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
- A01H6/822—Capsicum sp. [pepper]
Abstract
Provided are a plant which is capable of bearing seedless fruits in a stable state over several generations; a seedless fruit obtained from this plant; a method of producing a variety which can easily bear seedless fruits without fail; the variety produced by this production method; and a seedless fruit obtained by this variety thus produced. A plant capable of bearing seedless fruits characterized by being obtained by a mating method which comprises (a) a step wherein an individual of a male sterile line is mated with an individual of a parthenocarpic line and from individuals of the first filial generation thus obtained, an individual of the first filial generation which has the male sterility and parthenocarpy and is capable of bearing seedless fruits is selected; and (b) a step wherein the preceding individual of the first filial generation thus selected is mated with an individual of a fixed line, which is capable of sustaining the parthenocarpy and male sterility of the former individual, as a pollen parent to thereby give a progeny individual having the parthenocarpy and male sterility.
Description
Technical field
The no seed fruit that the present invention relates to produce the plant of no seed fruit, obtains from this plant, the no seed fruit of producing the preparation method of the kind of no seed fruit, the kind by this preparation method making and obtaining from the individuality of the kind of made.
The spy that the application requires on February 5th, 2008 to propose in Japan is willing to 2008-25424 number priority, and its content is incorporated in this specification.
Background technology
Aspect plants such as vegetables, fruit, pursue traditionally has better kind such as output performance, disease resistance, market preference degree always, and the wind of breed improvement is in vogue.Particularly, in recent years, also there is the tendency of the food materials that hobby can conveniently arrange in the consumer when hobby local flavor, outward appearance are good.For example, cooking or seed need be removed such troublesome operations from fruit when edible, this diminishes plant that green pepper, capsicum etc. contain unedible seed just as one of reason of the glamour of food materials.If the vegetables that do not have seed etc. can be provided from the beginning, then not only can satisfy the hobby of ordinary consumer, can also expect the efficient activity of a large amount of food and drink industries of using etc.Therefore, the general mood of attempting the kind of the no seed fruit of making production prevails.
Aspect the preparation method of no seed fruit, the whole bag of tricks is disclosed.For example, by making plant hormone act on common seed-bearing plant, the method for making no seed fruit.For watermelon, mainly use colchicine, and for grape, mainly use gibberellin.
On the other hand, aspect the preparation method of the kind of producing no seed fruit, the whole bag of tricks is disclosed.For example, the method such as the planting material of seed that (1) following production is used for no seed tomato, does not have the plant of the ability that produces the seed tomato or have the no seed tomato of generation or tomato plants is disclosed, described method comprises the steps: that a. provides first tomato plants that comprises pk, fs-complex (that is, a pk, fs-parent); B., second tomato plants that comprises pk, fs-complex (that is, the 2nd pk, fs-parent) is provided; C. in order to produce the planting material such as seed that comprises pk, fs-complex, and make the described first and second tomato plants mating; D. according to circumstances, the planting material that obtains is like this cultivated in the tomato plants with the ability that produces no seed tomato; E. according to circumstances, cultivate described tomato plants, until the no seed tomato of generation, and the so no seed tomato (reference example such as patent documentation 1) that obtains of results.In the method, at first make known no seed parent and non-no seed parent mating and the individual all self-pollinations in hybrid first filial generation.From the hybrid second filial individuality of such acquisition, select no seed or individuality that can not functional reproduction, count the self-pollination in generation repeatedly for wherein each it is settled out, thus the strain of making the no seed fruit of production.
Patent documentation 1: Japanese Unexamined Patent Application Publication 2003-501053 communique
Summary of the invention
Invent problem to be solved
Yet in the method for using plant hormone, the phase is applied to each individuality respectively with the plant hormone of appropriate amount in due course, and the problem of existence is to need excessive work.
On the other hand, in the method for above-mentioned (1), even making plant hormone act on each individual time and labor respectively in order to make the strain with the ability that produces no seed tomato can be solved, in order to carry out self-pollination, still need to carry out following numerous and diverse operation: the preferred manual closed pollen tube of opening parent's tomato plants, pollen is taken out from pollen tube, and it is pollinated in the gynoecium of parent's tomato plants.In addition, in order to make stable strain, be necessary to carry out repeatedly self-pollination, and continue number for cultivating, this still needs a large amount of time and labors.
The purpose of this invention is to provide: can go through number generation and stably produce the plant of no seed fruit, the no seed fruit that obtains from this plant, easy and make effectively the kind that can produce no seed fruit method, adopt the kind of this preparation method making and the no seed fruit that obtains from the kind of this making.
The method of dealing with problems
The inventor etc. further investigate for addressing the above problem, found that: the individuality by making the male sterility strain and the individuality of parthenocarpy strain carry out the hybrid first filial generation individuality that mating obtains having male sterility and parthenocarpy, and the individuality of strain that allows to keep this individual male sterility and parthenocarpy can access the offspring individual of having inherited these two kinds of phenotypes as pollen parent and this stud mating; And, backcross as pollen parent and this offspring individual by making with the individuality that this individuality has a stable strain of identical pollen parent, can make the kind of going through number generation and stably producing no seed fruit; Thereby finished the present invention.
That is, the invention provides the plant that can produce no seed fruit, it is to obtain by the mating method with following step (a)～(b); (a) from the hybrid first filial generation groups of individuals that the stud mating of the individuality by making the male sterility strain and parthenocarpy strain obtains, select the step that has male sterility and parthenocarpy and can produce the hybrid first filial generation individuality of no seed fruit; And (b) for the described hybrid first filial generation individuality of selecting, allow to keep the individuality of stable strain of this individual parthenocarpy and male sterility as pollen parent and this stud mating, obtain to have the step of offspring's individuality of parthenocarpy and male sterility.
In addition, the present invention also provides the plant that can produce no seed fruit, it is the individuality that obtains by the mating method that also repeats the following step (c) more than 1 time in described step (b) afterwards: (c) for described offspring's individuality of gained, backcross as pollen parent and this offspring's individuality with the individuality of the stable strain used as pollen parent in the step (b) again, obtain to have the step of offspring's individuality of parthenocarpy and male sterility.
In addition, the invention provides the plant that can produce no seed fruit, wherein, described stable strain is to cultivate and the strain of stabilisation by anther culture or ovule.
In addition, the invention provides the plant that can produce no seed fruit, wherein, this plant is green pepper (ピ one マ Application).
In addition, the invention provides the plant that can produce no seed fruit, wherein, described offspring's individuality with parthenocarpy and male sterility is the individuality that is selected from 3 body 74-2 (FERM BP-10935), 3 body 74-4 (FERM BP-10936) and their the offspring's individuality.
In addition, the invention provides the plant that can produce no seed fruit, wherein, described stable strain is body 74 (FERM BP-10934).
In addition, the invention provides no seed fruit, it is individuality solid the obtaining under without the situation of fertilization by making the aforesaid plant that can produce no seed fruit.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, this method comprises the individuality for the male sterility kind with parthenocarpy, and the individuality that allows to keep the stable strain of this individual parthenocarpy and male sterility is backcrossed as pollen parent and this individuality.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, described male sterility kind with parthenocarpy is the kind that obtains by the mating method with following step (a)～(b): (a) select the step that has male sterility and parthenocarpy and can produce the hybrid first filial generation individuality of no seed fruit from the hybrid first filial generation groups of individuals that the stud mating of the individuality by making the male sterility strain and parthenocarpy strain obtains; And (b) for the described hybrid first filial generation individuality of selecting, allow to keep the individuality of stable strain of this individual parthenocarpy and male sterility as pollen parent and this stud mating, obtain to have the step of offspring's individuality of parthenocarpy and male sterility.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, described male sterility kind with parthenocarpy is by having following step (a)～(c) and carrying out the kind that the mating method of the step (c) more than 1 time obtains repeatedly: (a) select the step that has male sterility and parthenocarpy and can produce the hybrid first filial generation individuality of no seed fruit from the hybrid first filial generation groups of individuals that the stud mating of the individuality by making the male sterility strain and parthenocarpy strain obtains; (b) for the described hybrid first filial generation individuality of selecting, allow to keep the individuality of stable strain of this individual parthenocarpy and male sterility as pollen parent and this stud mating, obtain to have the step of offspring's individuality of parthenocarpy and male sterility; And (c) for described offspring's individuality of gained, backcross as pollen parent and this offspring's individuality with the individuality of the stable strain used as pollen parent in the step (b) again, obtain to have the step of offspring's individuality of parthenocarpy and male sterility.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, described stable strain is to cultivate and the strain of stabilisation by anther culture or ovule.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, the described kind that can produce no seed fruit is green pepper.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, described male sterility kind with parthenocarpy is the kind that is selected from 3 body 74-2 (FERM BP-10935), 3 body 74-4 (FERM BP-10936) and their the offspring's kind.
In addition, the invention provides the preparation method of the kind that can produce no seed fruit, wherein, described stable strain is body 74 (FERM BP-10934).
In addition, the invention provides the kind of producing by aforementioned each the described preparation method that can produce the kind of no seed fruit.
In addition, the invention provides no seed fruit, thus its be make aforesaid kind individuality without fertilization solid obtaining.
The invention effect
The plant that can produce no seed fruit of the present invention has parthenocarpy, therefore, except not pollinating, only need cultivate according to a conventional method, namely can be conscientiously and stably produce the fruit that do not have seed (below be also referred to as " no seed fruit ").The fruit in the common stable strain used as pollen parent of backcrossing that the quality of the no seed fruit of producing, results performance etc. and being used for made plant of the present invention is close, thereby, can expect no seed fruit that plant production of the present invention goes out become be not only that market can be accepted, fully satisfied the fruit of needs especially.And, for plant of the present invention, only by backcrossing, can make the offspring's individuality that can produce no seed fruit easily.
In addition, can be the same with other stable strain stable and make easily and can produce the kind of no seed fruit by the preparation method that can produce the kind of no seed fruit of the present invention, and need not the individuality that expression purpose phenotype is selected on generation generation ground.That is, preparation method of the present invention is compared very efficient with conventional method, and also is preferred at economic aspect.
The embodiment of invention
In the present invention, " parthenocarpy " refers to: under the situation of pollination, fertilization, and the character of fruit growth hypertrophy.Usually, the fruit that obtains by parthenocarpy is the fruit that does not have seed.In some cases, according to the kind of plant, adjust planting environments such as temperature, sunshine time aptly, also can demonstrate parthenocarpy.That is, parthenocarpy has inheritance to determine, also has planting environment to determine, but the parthenocarpy among the present invention refers to what inheritance determined.For the plant of cultivating for edible purpose, the plant with parthenocarpy that preferred inheritance determines because its reliability and reappearance height, and can reduce the labour of management and cultivation environment.
In the present invention, " male sterility " refers to: that inheritance determines, cause owing to arrenotoky factors such as pollen and to be suppressed and can't to form the character of seed.Not having particular restriction for male sterility of the present invention, as long as finally be suppressed, can be the result who does not produce pollen itself fully, also can be the Insufficient result of pollen.In addition, can also be result owing to the factor beyond the pollen.Factor beyond this pollen for example has pollen tube obturation, stamen form not congruent.
The plant that can produce no seed fruit of the present invention obtains by following mating method, and described mating method has: (a) select the step that has male sterility and parthenocarpy and can produce the hybrid first filial generation individuality of no seed fruit from the hybrid first filial generation groups of individuals that the stud mating of the individuality by making the male sterility strain and parthenocarpy strain obtains; (b) for the hybrid first filial generation individuality of selecting in the step (a), allow to keep the individuality of stable strain of this individual parthenocarpy and male sterility as pollen parent mating with it, obtain having the step of offspring's individuality of parthenocarpy and male sterility.Below, describe set by step.
At first, as step (a), from the hybrid first filial generation groups of individuals that the stud mating of the individuality by making the male sterility strain and parthenocarpy strain obtains, select the hybrid first filial generation individuality that has male sterility and parthenocarpy, can produce no seed fruit.Particularly, from the hybrid first filial generation groups of individuals that obtains by the pollen to the individuality of the individuality of male sterility strain pollination parthenocarpy strain, select the individuality with male sterility and parthenocarpy.Individuality as the male sterility strain of using in the step (a), as long as have male sterility, it is not had particular determination, can use the individuality of known male sterility strain, can use the new individuality made from male sterility such as the known method that adopts anther culture yet.Similarly, the individuality for as the parthenocarpy strain does not have particular restriction, can use the individuality of known parthenocarpy improved line, can use the new individuality made from parthenocarpy such as the known method that adopts anther culture yet.
The hybrid first filial generation individuality of selecting like this has parthenocarpy, thereby normally can produce the individuality of no seed fruit.On the other hand, by with the stud mating of other kind, can obtain offspring individual.But different because of the situation of mating object, offspring individual might be able to not be inherited male sterility and the parthenocarpy that obtains with great difficulty.Thereby the individuality of the stable strain by allowing to keep this individual parthenocarpy and male sterility can be made the offspring's individuality with parthenocarpy and male sterility as pollen parent mating with it.Particularly, as step (b), the individuality of the stable strain that can keep parthenocarpy and male sterility by making the hybrid first filial generation individuality of selecting in the step (a) is as the hybrid first filial generation stud mating of selecting in pollen parent and the step (a), can obtain to have offspring's individuality of parthenocarpy and male sterility, i.e. the plant that can produce no seed fruit of the present invention (below be also referred to as " no seed fruit yielding ability plant ").
Here, there is not particular restriction for the individuality as pollen parent and the stable strain of the hybrid first filial generation stud mating selected, as long as it is the individuality of following stable strain, described stable strain is to bring into play the stable strain of function as pollen parent, and be to access the parthenocarpy that to keep this hybrid first filial generation individuality and the stable strain of male sterility, that is, can be by obtaining to have the stable strain of the hybrid second filial individuality of parthenocarpy and male sterility with this hybrid first filial generation stud mating.This can be the parthenocarpy strain as the stable strain (following also title " strain stablized in pollen parent ") that pollen parent carries out mating, can not be the parthenocarpy strain also, but be preferably the parthenocarpy strain.
By inference, any character in male sterility and the parthenocarpy all with a plurality of gene-correlations, but concrete phenotypic expression mechanism is still indeterminate.Therefore, male sterility and these two kinds of phenotypes of parthenocarpy that this hybrid first filial generation individuality that the stud mating of differentiation and which kind of strain can make the filial generation succession select under the condition of not carrying out the mating operation has are very difficult.Therefore, for any stable strain, by the pollen that the hybrid first filial generation individuality of selecting in the step (a) is pollinated and obtained from the individuality of various stable strains, come actual carry out mating and affirmation, thereby judge that its pollen parent that whether can be used as in the step (b) stablizes strain, this is the most practical at present, so preferred.
With individual the same as parent's hybrid first filial generation, offspring's individuality of Huo Deing also is the individuality that can produce no seed fruit like this, can stablize and obtain easily having offspring's individuality of parthenocarpy and male sterility by backcrossing.Particularly, as step (c), make to be used as pollen parent in the step (b) and to stablize the individuality of strain and backcross as pollen parent and gained offspring individuality, and repeat more than 1 time, can obtain to have offspring's individuality of parthenocarpy and male sterility.The number of repetition of backcrossing for step (c) does not have particular restriction, no seed fruit yielding ability plant of the present invention can be made to such an extent that go through number generation as other stable strain and stablizes.
In the making of no seed fruit yielding ability plant of the present invention, by using the individuality of stablizing strain as pollen parent, can guarantee mating result's reappearance, practical property.In addition, stablize strain as this pollen parent, the preferred use has homozygous stable strain.Still be parthenocarpy for male sterility no matter, all can think: performance phenotype easily when related gene is homozygote, and backcross easily.Like this have a stable strain that homozygous stable strain can be known pure lines, also can be the stable strain of making by traditional self-pollination, can also be to cultivate and the strain of stabilisation by anther culture or ovule.It is known preparation method that anther culture and ovule are cultivated, and can easy and promptly make the various stable strain that whole zygotes are homotype (homo).Stablize strain as the pollen parent that in preparation method of the present invention, uses, preferably cultivate and the strain of stabilisation by anther culture or ovule.
The preparation method that can produce the kind of no seed fruit of the present invention comprises that the individuality of the stable strain that can keep parthenocarpy and male sterility of the individuality that makes the male sterility kind with parthenocarpy backcrosses as pollen parent and the individuality with male sterility kind of parthenocarpy.In common backcrossing, be necessary from resulting offspring's groups of individuals, to select the individuality with purpose character, and in preparation method of the present invention, whole basically offspring's individualities all are the individualities with male sterility kind of parthenocarpy.Here, the male sterility kind with parthenocarpy in the preparation method of the present invention can be used for example no seed fruit yielding ability plant of the present invention.In addition, the stable strain of using as the pollen parent in the preparation method of the present invention that can keep parthenocarpy and male sterility can be used with the middle pollen parent that uses of described step (b) and stablize the identical strain of strain.
That is, no seed fruit yielding ability plant of the present invention and the kind that can produce no seed fruit that adopts preparation method of the present invention to make have parthenocarpy, by preventing pollination, and can be conscientiously and the no seed fruit of production expeditiously.In addition, pollinate by the pollen that uses the pollen parent that from make, uses to stablize the individuality collection of strain, can obtain to have offspring's individuality of male sterility and parthenocarpy easily.And, in the present invention, the method for pollinating there is not particular restriction, can adopt conventional method to carry out, but the pollen that preferred use is stablized the individuality collection of strain from pollen parent carry out artificial cross, because purpose pollen is pollinated.
Except having male sterility and parthenocarpy, no seed fruit yielding ability plant of the present invention and kind and congener other plant of adopting preparation method of the present invention to make do not have big difference, can under identical growth reproduction condition, adopt conventional method to cultivate.For controlled pollination, preferably in can carrying out the confined space of environmental management, plastic tunnel etc. cultivates.
With regard to the aspects such as the qualities such as taste of the no seed fruit of producing with regard to no seed fruit yielding ability plant of the present invention etc., results property, get over repeatedly backcrossing of number, just the fruit of strain stablized in the pollen parent of use in more approaching the backcrossing.Therefore, select to use pollen parent to stablize the kind of strain by suitable, can make the quality of the no seed fruit of producing, the property gathered in the crops etc. reach expectation.
Do not have particular restriction to no seed fruit yielding ability plant of the present invention and as the kind of the object of preparation method of the present invention, get final product so long as produce the plant of the fruit with seed, but the edible plant of preferably fruit.In addition, can be therophytes (1 year grass), also can be perennial plant (herba perennis).As this plant, for example green pepper, red pepper (パ プ リ カ), capsicum (ト ウ ガ ラ シ), eggplant (Na ス), tomato plants of Solanaceae such as (ト マ ト) etc. are arranged.As no seed fruit yielding ability plant of the present invention etc., be preferably green pepper, red pepper, capsicum, more preferably green pepper.
For example, aspect green pepper, at first, select by making known male sterility improved line green pepper " prosperous Jie of MS " and known parthenocarpy improved line green pepper " A3 " or " A7 " mating hybrid first filial generation individuality " SA3 " and " SA7 " that obtain, that can produce the no seed green pepper with male sterility and parthenocarpy.Then, by make to known parthenocarpy improved line green pepper " MIOGI (body お ぎ; the kind that can obtain from gardening research institute) " carry out anther culture and DH (the Double Haploid of " body (MI) 74 " etc., double haploid) strain (having homozygous stable strain) is backcrossed successively as pollen parent, can access the plant that " 3 body 74-2 ", " 3 body 74-4 " etc. produce no seed green pepper.And " 3 body 74-2 " carries out backcrossing for 2 times to " SA3 " with " body 74 " and obtains, and " 3 body 74-4 " carries out backcrossing for 4 times and obtain.In addition, use the pollen of gathering from " body 74 " to carry out mating by the individuality to " 3 body 74-2 ", " 3 body 74-4 ", can stably obtain to produce offspring's individuality of no seed green pepper.
Embodiment
By the following examples the present invention is carried out more specific description, but the present invention is not subjected to the restriction of following examples.And the cultivation of various green peppers is carried out according to conventional method.
＜have a selection of male sterility, parthenocarpy, the productive hybrid of no seed fruit first filial generation individuality 〉
At first, known male sterility green pepper " prosperous Jie of MS " is cultivated in the plastic tunnel that can prevent spontaneous pollination.
For each individual gynoecium of " the prosperous Jie of MS " that cultivates, award collection respectively and carry out mating from the pollen of known improved line " A3 ", obtained hybrid first filial generation individuality.From these hybrid first filial generation individualities, selected the hybrid first filial generation individuality " SA3 " that has male sterility and parthenocarpy and can produce no seed fruit.Use known improved line " A7 ", " A21 ", " A27 ", " A33 " to replace improved line " A3 " respectively, make it similarly and " prosperous Jie of MS " mating, selected the hybrid first filial generation individuality " SA7 ", " SA21 ", " SA27 ", " SA33 " that have male sterility and parthenocarpy and can produce no seed fruit respectively.
The making of＜no seed fruit yielding ability plant of the present invention 〉
At first, in order to use as stablizing strain with the pollen parent of the hybrid first filial generation stud mating of selecting, " MIOGI (body お ぎ) " carries out anther culture to known improved line, 6 DH strains have been made, i.e. " body 54 ", " body 64 ", " body 74 ", " body 144 ", " body 152 ", " body 159 ".Equally known improved line " soil assistant Ji R " is carried out anther culture, made 6 DH strains, i.e. " soil 33 ", " soil 55 ", " soil 57 ", " soil 71 ", " soil 119 ", " soil 120 ".And " MIOGI " and " soil assistant a Ji R " obtains seed and cultivates from Japanese gardening research institute and high county magistrate's economic combination meeting respectively.
Gather pollen from each the DH strain that obtains like this, carry out mating with " SA3 ", " SA7 ", " SA21 ", " SA27 ", " SA33 " these 5 hybrid first filial generation individualities respectively, obtained seed (offspring's individuality).These seeds are cultivated, make it solid without fertilization, the fertility of gained fruit is observed.
Consequently, 6 DH strains of " soil assistant Ji R " and the hybrid first filial generation individuality of 5 kinds offspring's individuality of mating gained have respectively all formed seed, all are not no seed fruit yielding ability plants.
On the other hand, to the hybrid first filial generation individuality of 6 DH strains of " MIOGI " and 5 kinds respectively mating and the fertility of offspring's individuality of obtaining investigate, the result is as shown in table 1.In the table, " zero " expression offspring individuality is sterility, and " * " expression offspring individuality is fertility, "-" expression free of data.
[table 1]
| Pollen parent | ||||||
| み54 | み64 | み74 | み144 | み152 | み159 | |
| SA3 | ○ | ○ | ○ | - | ○ | ○ |
| SA7 | ○ | ○ | ○ | - | ○ | ○ |
| SA21 | × | - | × | - | × | - |
| SA27 | × | - | × | - | × | - |
| SA33 | × | × | × | × | × | × |
As can be known: for " SA3 " and " SA7 ", by with " body 54 ", " body 64 ", " body 74 ", " body 152 ", " body 159 " these 5 strains as pollen parent, can access the offspring's individuality with parthenocarpy and male sterility, and these offspring's individualities are no seed fruit yielding ability plants.And, for these offspring's individualities, backcross as pollen parent with the individuality of the DH strain that is used separately as the pollen parent of making them, obtained seed (offspring's individuality).These seeds are cultivated, make it solid without fertilization, and the gained fruit is investigated, as can be known: identical with the parent, it is the no seed fruit yielding ability plant with parthenocarpy and male sterility.
Particularly, " SA3 " with " body 54 ", " SA3 " and " body 74 ", " SA3 " and " body 152 ", " SA7 " and " body 54 ", " SA7 " and " body 64 " and " SA7 " and " body 152 " mating and offspring's individuality of obtaining respectively, obtained the offspring's individuality until the 3rd generation by backcrossing, it all is the no seed fruit yielding ability plant with male sterility.In addition, the no seed green pepper that obtains from these no seed fruit yielding ability plants is aspect quality such as taste, local flavor and results property, all close with " MIOGI " green pepper as pollen parent, these no seed fruit yielding ability plants have good adaptability as edible cultivar.In the no seed fruit yielding ability plant of gained, the progeny plants called after " 3 body 74 " that will obtain by the mating of " SA3 " and " body 74 ".And, for " 3 body 74 ", by being that pollen parent is backcrossed repeatedly with " body 74 ", obtained progeny plants.These progeny plants all are no seed fruit yielding ability plant.
To stablize strain be the new plant that the inventor etc. makes for the no seed fruit yielding ability plant of Huo Deing and pollen parent thereof like this.Thereby the inventor etc. are preserved in the biological preservation of Independent Administrative Leged Industrial Technology Complex Inst's patent center with the seed of " 3 body 74-2 " in these progeny plants and " 3 body 74-4 " and the seed of stablizing " body 74 " of strain as their pollen parent as new plant.The preserving number aspect, " body 74 " is FERMBP-10934, and " 3 body 74-2 " is FERM BP-10935, and " 3 body 74-4 " is FERMBP-10936.And preservation day is for putting down on November 28th, 19.
Industrial applicibility
For no seed fruit yielding ability plant of the present invention and the kind that adopts preparation method of the present invention to make, simply by making it solid without fertilization, can obtain no seed fruit easy and effectively, thereby particularly the cultivation field at the edible fruit with seed can be used.
Claims (5)
1. can produce the preparation method of the capsicum veriety of no seed fruit, wherein, obtain having the male sterility capsicum veriety of parthenocarpy by the mating method that has following step (a)～(c) and carry out the step (c) more than 1 time repeatedly:
(a) step that selection has male sterility and parthenocarpy and can produce the hybrid first filial generation capsicum class individuality of no seed fruit from the hybrid first filial generation groups of individuals that obtains by the capsicum class that makes male sterility strain capsicum class stud mating individual and the parthenocarpy strain;
(b) for the described hybrid first filial generation capsicum class individuality of selecting, allow to keep the capsicum class individuality of stable strain of this individual parthenocarpy and male sterility as pollen parent and this stud mating, obtain to have the step of the offspring capsicum class individuality of parthenocarpy and male sterility; With
(c) for the described offspring capsicum class individuality of gained, to backcross with it as pollen parent with the capsicum class individuality of the stable strain used as pollen parent in the step (b) again, acquisition has the step of the offspring capsicum class individuality of parthenocarpy and male sterility;
Wherein step b) and c) in be the parthenocarpy strain as the stable strain of pollen parent.
2. the preparation method that can produce the capsicum veriety of no seed fruit according to claim 1, wherein, described stable strain is to cultivate and the strain of stabilisation by anther culture or ovule.
3. according to each the described preparation method that can produce the capsicum veriety of no seed fruit in the claim 1～2, wherein, the described capsicum veriety that can produce no seed fruit is green pepper.
4. the preparation method that can produce the capsicum veriety of no seed fruit according to claim 3, wherein, to be selected from preserving number be that 3 body 74-2, the preserving number of FERM BP-10935 is the 3 body 74-4 of FERM BP-10936 and the kind in their offspring's kind to described male sterility capsicum veriety with parthenocarpy.
5. the described preparation method that can produce the capsicum veriety of no seed fruit of claim 4, wherein, described stable strain is that preserving number is the body 74 of FERM BP-10934.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2008025424 | 2008-02-05 | ||
| JP025424/08 | 2008-02-05 | ||
| PCT/JP2009/051360 WO2009098983A1 (en) | 2008-02-05 | 2009-01-28 | Plant capable of bearing seedless fruits and method of producing variety bearing seedless fruits |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101932229A CN101932229A (en) | 2010-12-29 |
| CN101932229B true CN101932229B (en) | 2013-10-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009801039701A Active CN101932229B (en) | 2008-02-05 | 2009-01-28 | Plant capable of bearing seedless fruits and method of producing variety bearing seedless fruits |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8957286B2 (en) |
| EP (1) | EP2245922B1 (en) |
| JP (1) | JP5720096B2 (en) |
| KR (1) | KR101563225B1 (en) |
| CN (1) | CN101932229B (en) |
| AU (1) | AU2009211800B2 (en) |
| BR (1) | BRPI0907083A8 (en) |
| CA (1) | CA2714016A1 (en) |
| ES (1) | ES2580166T3 (en) |
| IL (1) | IL207377A (en) |
| MX (1) | MX2010008524A (en) |
| WO (1) | WO2009098983A1 (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5272071B2 (en) * | 2009-03-05 | 2013-08-28 | 横浜植木株式会社 | Nucleusless Capsicum Annuum and its production method |
| KR101241221B1 (en) | 2010-12-06 | 2013-03-13 | 주식회사 이지트로닉스 | Charging system for mild hybrid vehicle |
| NL2005908C2 (en) * | 2010-12-22 | 2012-06-28 | Rijk Zwaan Zaadteelt En Zaadhandel Bv | Fruit formation in the absence of fertilisation. |
| MX2014006167A (en) | 2011-11-22 | 2014-06-19 | Seminis Vegetables Seeds Inc | Seedless pepper plants. |
| CN102668977A (en) * | 2012-06-12 | 2012-09-19 | 重庆市农业科学院 | Method for cultivating seedless eggplant variety |
| USPP26127P3 (en) | 2012-12-06 | 2015-11-24 | Syngenta Participations Ag | Capsicum annuum plant named ‘SLP2B322’ |
| CN103444552B (en) * | 2013-09-28 | 2015-08-19 | 武汉市蔬菜科学研究所 | A kind of method of inducing eggplant flower pesticide regeneration haplobiont |
| JP6191996B2 (en) | 2014-01-17 | 2017-09-06 | 国立研究開発法人農業・食品産業技術総合研究機構 | Participatory outcome control genes and their use |
| JP6300215B1 (en) * | 2017-04-27 | 2018-03-28 | 節三 田中 | Methods for enhancing plant properties |
| CN113365494B (en) | 2018-12-13 | 2023-12-15 | 纽海姆有限公司 | Eggplant plant capable of generating fruit formation of seed abortion |
| CN114144059B (en) | 2019-07-22 | 2023-10-20 | 植物保护品种协会 | Composition for preventing seed formation in fruits |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008152134A1 (en) * | 2007-06-13 | 2008-12-18 | Zeraim Gedera Ltd. | Improved pepper plant |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1057401A1 (en) | 1999-06-04 | 2000-12-06 | Western Seed Espana S.A. | Seedless tomato and method for producing a seedless tomato, hybrid tomato plants capable of producing said seedless tomatoes and cultivation material therefore, and food products obtained from said seedless tomatoes |
-
2009
- 2009-01-28 KR KR1020107017061A patent/KR101563225B1/en active IP Right Grant
- 2009-01-28 BR BRPI0907083A patent/BRPI0907083A8/en not_active Application Discontinuation
- 2009-01-28 CA CA2714016A patent/CA2714016A1/en not_active Withdrawn
- 2009-01-28 CN CN2009801039701A patent/CN101932229B/en active Active
- 2009-01-28 JP JP2009552444A patent/JP5720096B2/en active Active
- 2009-01-28 WO PCT/JP2009/051360 patent/WO2009098983A1/en active Application Filing
- 2009-01-28 AU AU2009211800A patent/AU2009211800B2/en active Active
- 2009-01-28 US US12/865,962 patent/US8957286B2/en active Active
- 2009-01-28 MX MX2010008524A patent/MX2010008524A/en unknown
- 2009-01-28 EP EP09708741.5A patent/EP2245922B1/en active Active
- 2009-01-28 ES ES09708741.5T patent/ES2580166T3/en active Active
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2010
- 2010-08-03 IL IL207377A patent/IL207377A/en active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008152134A1 (en) * | 2007-06-13 | 2008-12-18 | Zeraim Gedera Ltd. | Improved pepper plant |
Non-Patent Citations (3)
| Title |
|---|
| Charles W B,et al.Seedlessness in capsicum-annuum-var-longum.《Journal of horticultural science》.1979,第54卷(第2期), * |
| Keiko Ishikawa,et al.High beta-carotene and capsaicinoid contents in seedless fruits of "shishitoh" pepper.《Hortscience》.2004,第39卷(第1期),153-155. * |
| Subodh Joshi,et al.Perspectives of bell pepper breeding.《Journal of new seeds》.2005,第6卷(第2-3期),51-73. * |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2009211800A1 (en) | 2009-08-13 |
| MX2010008524A (en) | 2010-10-04 |
| CA2714016A1 (en) | 2009-08-13 |
| US8957286B2 (en) | 2015-02-17 |
| ES2580166T3 (en) | 2016-08-19 |
| KR101563225B1 (en) | 2015-10-26 |
| EP2245922A1 (en) | 2010-11-03 |
| BRPI0907083A8 (en) | 2017-08-22 |
| AU2009211800B2 (en) | 2014-09-04 |
| WO2009098983A1 (en) | 2009-08-13 |
| CN101932229A (en) | 2010-12-29 |
| BRPI0907083A2 (en) | 2015-07-14 |
| US20100333228A1 (en) | 2010-12-30 |
| JP5720096B2 (en) | 2015-05-20 |
| EP2245922B1 (en) | 2016-04-27 |
| IL207377A0 (en) | 2010-12-30 |
| KR20100123826A (en) | 2010-11-25 |
| IL207377A (en) | 2014-11-30 |
| JPWO2009098983A1 (en) | 2011-05-26 |
| EP2245922A4 (en) | 2011-06-01 |
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