CN105248271B - A kind of method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment - Google Patents

Abstract

A kind of method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment, it is characterised in that：The Lubao 60 tian G5 in SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2, SUZHOUQING(sic) type tender flower stalk self-mating system G11 and Guangdong cabbage heart conventional variety is selected to comprise the following steps as parent material：Using G5 be maternal and Q2 is male parent, and hybridization obtains first-filial generation seed 1F1；Using G5 be recurrent parent and 1F1 as male parent, backcrossing obtain BC1F1；It is selfed by BC1F1 and obtains BC1F2；The compact plant selfing of plant type is selected to obtain BC1F3 in BC1F2；2F1 is obtained with BC1F3 hybridization using G11 to be maternal, BC1F3 selfings obtain BC1F4；Using 2F1 to be maternal 3F1 is obtained with BC1F4 hybridization；The compact plant selfing of plant type is selected to obtain 3F2 in 3F1；Compact plant type, upright blade are selected in 3F2, obtains 3F3 without the glossy dark green plant selfing of lateral bud and a kind of sedge color.The upright compact germ plasm resource of tender flower stalk of the present invention is not affected by environment in plantation, has gathered without lateral bud, the glossy dark green wax powder-free of a kind of sedge color and the best possible merchandise character genes such as petiole is shorter.

Description

A kind of method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment

Technical field

The present invention relates to vegetable breeding field, and in particular to a kind of upright compact germplasm money of tender flower stalk not affected by environment The method for creating in source.

Background technology

Tender flower stalk belongs to Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies tender flower stalk mutation in Plant Taxonomy, also known as Cabbage heart, dish point, dish luxuriant, edible organs are colored a kind of sedge and a kind of sedge leaf of attachment, are the Specialty vegetables for originating in China, are common people's eatings One of vegetable species.During cultivated since long, some are formd dedicated for producing the local varieties group of tender flower stalk product, such as The purple flowering stalk in Hubei, the cabbage heart in Guangdong, in addition with some other local varieties, such as knife of Shanghai one is neat, Yangzhou tender flower stalk.

The blade of usual plant is irradiated more multi-biomass accumulation by effective light also can be more, therefore, mould preferable strain Type is the high-quality premise of high crop yield, and for the larger tender flower stalk of planting density, upright compact plant is high yield and high quality Ideotype because interfered with each other between plant it is few, can make full use of luminous energy and divulge information, disease it is relative understand it is less.It is existing Some upright compact germ plasm resource quantity of tender flower stalk is few and character is easily affected by environment and changes, and there is presently no one The method that kind initiative is not easy upright compact germ plasm resource affected by environment.Although some tender flower stalk kinds declare that plant type is uprightly tight Gather, but find that the blade between the most of plant in tender flower stalk kind field applied at present is mutual in the middle and later periods according to our actual observations Block, improper ventilation is easily susceptible, and universal commodity rate is than relatively low.Upright Compactness is shown during only a few kind benign climate, But when environmental change especially in high temperature season, plant type just becomes loose, and commodity is remarkably decreased, tender flower stalk production is seriously constrained The further raising of amount and quality, the plant type of existing kind are necessary to be improved.Furthermore sprouting of lateral bud character is the weight of tender flower stalk One of commodity property is wanted, requires that lateral bud will not sprout or sprout as far as possible unobvious in tender flower stalk harvesting or first flower in production, And existing germ plasm resource includes cultivar sprouting of lateral bud character and easily influenceed by factors such as environment, sprouting of lateral bud trait expression is unstable It is fixed to be not easy tender flower stalk affected by environment without lateral bud germ plasm resource, it is necessary to formulate.

Tender flower stalk breeding at present is main or based on the traditional approach such as line breeding, conventional cross-breeding, by for many years Seed selection, the most resistance level of these conventional varieties is not high enough, and water and fertilizer utilization is low, and the space that yield and quality further improves is very It is small.Numerous researchs show that tender flower stalk there is obvious hybrid vigour, extremely can significantly increase output increased resistance, using hybrid Heterosis breeding mode is the inevitable choice of tender flower stalk breeding work from now on.In the practice process of breeding for heterosis, tender flower stalk is found Most of cross combination plant types become more to carry out, and sprouting of lateral bud is more, and petiole is substantially elongated, and commodity is remarkably decreased, and especially exists It can aggravate to show under poor environment, be difficult to apply in production.In order to solve this key technical problem, that currently mainly takes arranges It is that Parent petiole is short, plant type is upright compact for selection to apply, due to current tender flower stalk germplasm in strict conformity with this require still very Lack, generally effect is also not fully up to expectations, it is necessary to the upright compact germplasm money of a large amount of initiatives tender flower stalk not affected by environment Source, to adapt to the demand of tender flower stalk heterosis breeding.

Suzhou common people are known as the custom that SUZHOUQING(sic) type common white cauliflower a kind of sedge is eaten in early spring, be commonly called as dish point, due to Common Chinese cabbage needs low temperature long-day vernalization ability bolting, typically is starting to list by the end of February, the supply phase is short, only 1 month.For In other seasons also there is tender flower stalk listing, introduced a fine variety the tender flower stalk for originating in south China in recent years（That is Guangdong cabbage heart）, due to mouthfeel with The dish point of this real estate has obvious difference, and market acceptance is not high, development is more slow, and existing tender flower stalk kind is difficult to solve This technical problem, it is necessary to which seed selection is adapted to the kind of Suzhou consumption habit to solve above-mentioned technical problem, it is necessary to which initiative is adapted to Soviet Union The germ plasm resource of mouthfeel is consumed in state, to meet breeding demand.

In summary, in order to significantly improve flower stalk yield quality, it is necessary to which a large amount of tender flower stalk not affected by environment is straight without lateral bud Vertical compact germ plasm resource, there is presently no the open report of correlation technique, secondly disappears as breeding basis in order to expand tender flower stalk Expense region is also required to formulate the germ plasm resource of suitable local consumption demand, how they to condense together at present also without related side The open report of method, it is necessary to independently invent a kind of wound of tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud Method processed.

The content of the invention

The invention provides a kind of method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment.Using wide Eastern cabbage heart conventional variety Lubao 60 tian（Code name is G5）, SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2 and SUZHOUQING(sic) type dish A kind of sedge self-mating system G11 is as parent material, using traditional breeding method and a variety of progeny selection methods such as hybridization and backcrossing, by strain Type is compact, upright blade, first flower when lateral bud do not sprout and be adapted to the Suzhou consumption character such as mouthfeel to be collected at same germplasm materials In, tender flower stalk not affected by environment has been formulated without the upright compact germ plasm resource of lateral bud, successfully to select the new of high yield and high quality Kind has established Germplasm Bases.

To reach above-mentioned purpose, the technical solution adopted by the present invention is：A kind of tender flower stalk not affected by environment is uprightly compact The method for creating of type germ plasm resource, select Guangdong cabbage heart conventional variety Lubao 60 tian, the selfing of SUZHOUQING(sic) type common Chinese cabbage Be Q2 and SUZHOUQING(sic) type tender flower stalk self-mating system G11 as parent material, including at least the following steps：

The first step, using the Guangdong cabbage heart conventional variety Lubao 60 tian as maternal and described SUZHOUQING(sic) type common white Dish self-mating system Q2 is paternal hybrid, obtains first-filial generation seed 1F1；

Second step, it is male parent backcrossing by recurrent parent and the first-filial generation 1F1 of the Lubao 60 tian, is returned Hand over the first-filial generation seed BC1F1 of a generation；

3rd step, the second filial generation seed of first backcross generation is obtained with the first-filial generation BC1F1 selfings of the first backcross generation BC1F2；

4th step, the plant selfing for selecting plant type compact in the second filial generation BC1F2 of the first backcross generation are returned Hybridization three generations's seed BC1F3 of a generation；

5th step, obtained using the SUZHOUQING(sic) type tender flower stalk self-mating system G11 to be maternal with the hybridization three generations BC1F3 hybridization First-filial generation seed 2F1, the hybridization three generations BC1F3 selfings of the first backcross generation obtain the hybridization four generations seed of first backcross generation BC1F4；

6th step, obtained newly with the hybridization of the first backcross generation four generations BC1F4 hybridization using the first-filial generation 2F1 to be maternal First-filial generation seed 3F1；

7th step, the compact plant selfing of plant type is selected to obtain second filial generation seed 3F2 in the first-filial generation 3F1；

8th step, selected in the second filial generation 3F2 plant type is compact, upright blade, first flower when lateral bud do not sprout and It is tender flower stalk not affected by environment that the glossy dark green plant selfing of a kind of sedge color, which obtains hybridization three generations seed 3F3, hybridization three generations's seed 3F3, Upright compact germ plasm resource；

The morphological feature of the upright compact germ plasm resource of tender flower stalk is that plant type is compact, upright blade, and lateral bud is not during first flower Sprout, plant height is 36 ~ 38 centimetres, and strain width is 19 ~ 21 centimetres, and the glossy dark green wax powder-free of a kind of sedge color, main a height of 23 ~ 25 centimetres of a kind of sedge, main a kind of sedge is thick For 1.5 ~ 2.5 centimetres, tender flower stalk weighs 59 ~ 62 grams；Blade grows 24.5 ~ 26 centimetres, and blade is wide 14 ~ 16 centimetres, and petiole grows 5.5 ~ 7 lis Rice, leaf green is glossy subcircular, a kind of sedge leaf petiole and a kind of sedge angle are small, and blade face is open and flat small with a kind of sedge angle.

Relevant content in above-mentioned technical proposal is explained as follows：

1st, in such scheme, the morphological feature of the Guangdong cabbage heart conventional variety Lubao 60 tian is 26 ~ 28 lis of plant height Rice, 28 ~ 34 centimetres of strain, base leaf blade oval, bottle green, blade grow 17 ~ 19 centimetres, and blade is wide 7.5 ~ 9 centimetres, petiole length It is 5 ~ 6.5 centimetres, wide 0.9 ~ 1.5 centimetre；A kind of sedge leaf is narrow avette, and tender flower stalk consolidation bar is even, and dark green glossy, main a kind of sedge is high 22 ~ 26 centimetres, main 1.5 ~ 1.8 centimetres of a kind of sedge transverse diameter, tender flower stalk weighs 35 ~ 40 grams.The source of the Lubao 60 tian is that the Guangdong got is introduced a fine variety from Guangzhou Cabbage heart conventional variety, by the seed selection of Guangzhou vegetables Science Institute.

2nd, in such scheme, the morphological feature of the SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2 is 17 ~ 18 centimetres of plant height, 24 ~ 25 centimetres of strain, 278 ~ 283 grams of Weight per plant, the number of blade are 20 ~ 22, and property of girdling the waist is good, blade subcircular, petiole cochlear, leaf Piece bottle green, the green white of petiole；During bolting, a kind of sedge green has draped over one's shoulders wax powder, and tender flower stalk plant type compact half is upright, petiole and internode away from short, A kind of sedge leaf petiole and a kind of sedge angle are medium, and blade face is open and flat medium with a kind of sedge angle.The SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2's comes Source is the excellent common Chinese cabbage individual plant that my unit is selected from Suzhou farmers' vegetable plot, after multigeneration system seed selection, than original Colony's regularity greatly improves.

3rd, in such scheme, the morphological feature of the SUZHOUQING(sic) type tender flower stalk self-mating system G11 is compact for plant type, and plant height 32 ~ 34 centimetres, 23 ~ 25 centimetres of strain；There is wax powder on a kind of sedge green surface, and tender flower stalk weighs 49 ~ 51 grams, and thick 1.5 ~ 2.2 centimetres of a kind of sedge, a kind of sedge is high by 21 ~ 22 Centimetre；Leaf color is green, blade subcircular, and blade grows 15 ~ 16 centimetres, and blade is wide 9 ~ 11 centimetres, and petiole grows 5 ~ 6 centimetres, and petiole is wide by 1.5 ~ 2.0 centimetres, a kind of sedge leaf petiole and a kind of sedge angle are small, and blade face is open and flat big with a kind of sedge angle.The SUZHOUQING(sic) type tender flower stalk self-mating system G11's Source is the plant for the easy bolting that my unit is found from the common Chinese cabbage vegetable plot of Suzhou, by inbreeding of more generation optimum selecting, is obtained The stable common Chinese cabbage type tender flower stalk self-mating system of major traits, produced available for local winter tender flower stalk.

4th, in such scheme, hybridization three generations's seed 3F3 is the upright compact germplasm money of tender flower stalk not affected by environment Source, the now required plant type gathered is compact, upright blade, first flower when lateral bud do not sprout and be adapted to the characters such as Suzhou consumption mouthfeel Lower generation can be entailed by having stablized, but now gene is not homozygous, and other important Agronomics are also inconsistent, can root According to needing further preferentially to purify, the donor parents that can act also as merit gene are used in selection cross.

5th, in such scheme, germplasm refers to determine biological heredity character, and its hereditary information is transmitted into offspring's from parental generation Inhereditary material, it is the entity of objective reality, gene is referred to as on science of heredity.There is value in heredity, breeding and production All living things material can be referred to as germ plasm resource, be to cultivate the material base of new varieties from germ plasm resource for the angle of breeding Plinth, the form of expression can be：Local varieties, improve the breed, the kind of new seed selection, introduced variety, seed, mutant, wild species, Kindred plant, the various biotypes of artificial creation, vegetative propagation organ, individual cells, individual chromosome, individual gene, very To DNA fragmentation etc..

6th, in such scheme, backcrossing refers to two parents to hybridize again with one of parent being hybridized.For The parent being repeatedly returned is referred to as recurrent parent, and recurrent parent is the object for receiving improvement, is beneficial traits（Objective trait）Connect Receptor, also known as receptor parent, the parent applied when only hybridizing for the first time, it is the supplier of objective trait, therefore claims donor parent This.

7th, in such scheme, the tender flower stalk belongs to Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage on botany classification Subspecies tender flower stalk mutation, it is main to include the cabbage heart originating from South China（Guangdong cabbage heart）Regional purple flowering stalk, Chinese cabbage with Hunan Hubei Province etc. A kind of sedge and other areas are to harvest local varieties of the tender flower stalk as main purpose.Tender flower stalk can be subdivided into green dish according to the color on a kind of sedge surface A kind of sedge, purple flowering stalk and Chinese cabbage a kind of sedge, tender flower stalk of the present invention are green tender flower stalk.Tender flower stalk can be divided into main a kind of sedge and side according to a kind of sedge inserted part A kind of sedge, main a kind of sedge are formed for terminal bud germination, and side a kind of sedge is formed for sprouting of lateral bud, and tender flower stalk of the present invention is the type of edible main a kind of sedge.

8th, in such scheme, the common Chinese cabbage belongs to Cruciferae Brassica genus Vegetable Crops of Brassica not balling on botany classification Chinese cabbage subspecies common Chinese cabbage mutation, heretofore described common Chinese cabbage refer specifically to SUZHOUQING(sic) type.

Due to the utilization of above-mentioned technical proposal, design of the invention and have the beneficial effect that：It is straight without lateral bud for current tender flower stalk Vertical compact germ plasm resource is easily affected by environment, heterosis breeding needs upright compact germ plasm resource not affected by environment and Lack the technical problems such as the tender flower stalk germ plasm resource of suitable Suzhou consumption mouthfeel, this invention contemplates a kind of dish not affected by environment The upright compact germ plasm resource of a kind of sedge, required merit is gathered in same germplasm materials.Different parent materials have Respective advantage and disadvantage, wherein, it is contemplated that SUZHOUQING(sic) type common Chinese cabbage advantage is mainly compact with a tight waist, a kind of sedge leaf petiole of plant type and flower The angle of stem is smaller, petiole is short, leaf subcircular and mouthfeel are adapted to Suzhou District etc., and shortcoming mainly needs spring low temperature long-day Changing could bolting be bloomed, breeding time is long, often there is wax powder on a kind of sedge and blade face and pitch is shorter etc.；SUZHOUQING(sic) type tender flower stalk advantage and Suzhou Blue or green type common Chinese cabbage is similar, it is easier to which bolting, shortcoming are that a kind of sedge and blade face often have wax powder, and life is compared with the slow cabbage heart for originating from Guangdong It is long-term obvious partially long；Cabbage heart advantage originating from South China is bolting without low temperature long-day, the glossy dark green wax powder-free of a kind of sedge color, section length Moderate, shortcoming is that upright compact plant germplasm is few, lateral bud more or less substantially sprouts and these characters are often easily affected by environment.Cause This, we are by the use of SUZHOUQING(sic) type common Chinese cabbage, SUZHOUQING(sic) type tender flower stalk and Guangdong cabbage heart as parent, using hybridization and backcrossing Deng traditional breeding method and a variety of progeny selection methods, lateral bud is not sprouted and is adapted to Suzhou during compact, first flower that plant type is upright The characters such as consumption mouthfeel are collected in same germplasm materials, have formulated tender flower stalk not affected by environment without the upright compact of lateral bud Germ plasm resource.

By our multiple field plantings, high temperature season is made comparisons with favorable season, hence it is evident that the dish that the visible present invention formulates A kind of sedge germ plasm resource still keeps lateral bud not sprout being not apparent from elongating with upright compact plant, petiole and section length, and traditional dish The upright compact germ plasm resource of a kind of sedge seems that plant type is carried out at high temperature（Petiole substantially becomes big with scape angle）, petiole and internode are bright It is aobvious to elongate, illustrate that the tender flower stalk that the present invention formulates is not affected by environment without the upright compact germ plasm resource of lateral bud, while also gather Many merit genes such as the best possible merchandises such as the glossy dark green wax powder-free of a kind of sedge color and petiole are shorter and suitable Suzhou consumption mouthfeel.

The characteristic of the present invention is artificially to break geographical estrangement so that the common Chinese cabbage and tender flower stalk gene of different ecological area Merged into each other, formulated a kind of upright compact germ plasm resource of tender flower stalk not affected by environment, enrich tender flower stalk germplasm money Source storehouse, the new route of a tender flower stalk germplasm innovation is started.

Tender flower stalk belongs to Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies tender flower stalk mutation in Plant Taxonomy, specially To spend the vegetables of a kind of sedge and a kind of sedge leaf as edible tissue, the wherein cabbage heart type only province's whole year sown area of Guangdong one is no less than 200,000 for production Hectare, be the maximum vegetables of province sown area, need about 1500 tons of seed year, due to it is most of be conventional seed, the output value is about 30000000 yuan.Lateral bud when the upright compact germ plasm resource of tender flower stalk of the present invention has gathered compact plant type, upright blade, first flower simultaneously Do not sprout, the merit gene such as the shorter and suitable Suzhou consumption mouthfeel of the glossy dark green wax powder-free of a kind of sedge color, petiole, and these characters are not Variable rate technology is stable when being planted with season, and it is not affected by environment can to stablize heredity.If formulated using the present invention high-quality tight Gather type germ plasm resource, in conjunction with heterosis breeding technology, seed selection combination, then the market share may surpass hundred million yuan.With Common people from having enough to eat in the past to eating good things, eat rare and eat healthy etc. to vary the diet the stream of direction transformation and population Dynamic property enhancing, many local characteristic vegetables are cultivated with being no longer limited only to main product, are introduced a fine variety to domestic and international, wherein tender flower stalk development is outstanding It is fast, and consumption market constantly expands, both at home and abroad can be increasing for tender flower stalk high quality seed demand, therefore, of the invention Application prospect can also become better and better.

Brief description of the drawings

Accompanying drawing 1 is initiative flow chart of the tender flower stalk of the embodiment of the present invention without the upright compact germ plasm resource of lateral bud；

Accompanying drawing 2 is that the tender flower stalk of the embodiment of the present invention shines without the upright compact germ plasm resource field individual plant of lateral bud；

Accompanying drawing 3 be the embodiment of the present invention tender flower stalk without the upright compact germ plasm resource field of lateral bud integrally according to one；

Accompanying drawing 4 be the embodiment of the present invention tender flower stalk without the upright compact germ plasm resource field of lateral bud integrally according to two.

Embodiment

Needed to formulate the initiative target of new germ plasm resource according to current tender flower stalk breeding.

In the following description, involved parent material SUZHOUQING(sic) type common Chinese cabbage Q2 and SUZHOUQING(sic) type tender flower stalk G11 is the own breeding material of our unit, and parent material Lubao 60 tian is the cabbage heart conventional variety quoted from Guangzhou.

Agricultural Science Inst. of Taihu District of Jiangsu Prov.（Academy of Agricultural Sciences of Suzhou City）Proceeded by winter in 2007 not by Initiative work of the tender flower stalk without the upright compact germ plasm resource of lateral bud that environment influences, such germplasm has been filled up by years of researches The blank of method for creating is as follows referring to the flow chart shown in accompanying drawing 1, specific implementation step：

In December, 2007 is in the live SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2 of greenhouse, 3 square metres of plot area, final singling 60 Strain；2 months 2008 in the live Guangdong cabbage heart conventional variety Lubao 60 tian of greenhouse（Code name G5）, 3 square metres of plot area, determine 100 plants of seedling；Florescence in April, 2008 selects the short individual plant of 5 plants of robust growth petioles to remove stamen as female parent in G5, in Q2 Select the individual plant of 5 plants of robust growths to pollinate as male parent to hybridize, seed maturity mixing harvesting obtains first-filial generation seed 1F1.

In August, 2008 is in the live first-filial generation seed 1F1 and G5 of greenhouse, 3 square metres of plot area, 100 plants of final singling；October Florescence selects the short individual plant of 5 plants of robust growth petioles to remove stamen as maternal, 5 plants of leaves of selection in first-filial generation F1 in G5 The individual plant of the short robust growth of handle is pollinated as male parent to be hybridized, and seed maturity mixing harvesting obtains the first-filial generation kind of first backcross generation Sub- BC1F1.

The 2 months 2009 first-filial generation seed BC1F1 in the live first backcross generation of greenhouse, 3 square metres of plot area, final singling 100 plants, select that 10 plants of petioles are short, leaf a kind of sedge color is glossy dark green, blade subcircular is similar with growing way stalwartness breeding time wherein in the neat mouth phase Individual plant, remaining plant pulls out, and bagging selfing, picking time harvests respectively, and every plant takes about 100 seeds are well mixed to be returned The second filial generation seed BC1F2 of a generation.

In August, 2009 is in the second filial generation seed BC1F2 of the live first backcross generation of greenhouse, and 9 square metres of plot area, final singling is about 300 plants, find there is that more than 10 strain petioles are especially short, leaf a kind of sedge color is glossy dark green and the individual plant of blade subcircular, overall growing way are weak in the neat mouth phase In other plant, remaining individual plant is pulled out, above-mentioned plant is put into fly net, October, full-bloom stage was every 2 days individual plant difference indirect labors Pollination 2 times, seed maturity harvests respectively, and every plant takes the well mixed hybridization three generations's seed for obtaining first backcross generation of about 50 seeds BC1F3。

In December, 2009,2 months next years were in the live first backcross generation of greenhouse in greenhouse live SUZHOUQING(sic) type tender flower stalk self-mating system G11 Hybridization three generations seed BC1F3, plot area is 3 square metres, 100 plants of final singling, and 5 plants of plant types are selected out of G11 in the neat mouth phase Individual plant compact, that petiole handle angle is minimum and growing way is healthy and strong is stand-by, and remaining is pulled out, and 10 plants of plant types are selected from BC1F3 in the neat mouth phase Individual plant particularly compact, that petiole is especially short, leaf a kind of sedge color is glossy dark green, blade subcircular and growing way are healthy and strong is stand-by, and remaining is pulled out, April Florescence hybridizes by individual plant that is maternal and being chosen using BC1F3 of the individual plant that G11 chooses as male parent producing Crossbred, seed maturity difference Harvesting, each hybridization take about 100 seeds uniformly to mix Unified number and obtain first-filial generation seed 2F1.The same period will choose 10 Strain BC1F3 is covered before the early flowering season is entered with fly net, and October, full-bloom stage was pollinated 2 times every 2 days individual plant difference indirect labors, seed Maturity period harvests respectively, and every plant takes the well mixed hybridization four generations seed BC1F4 for obtaining first backcross generation of about 50 seeds.

In August, 2010 is in the live first-filial generation seed 2F1 of greenhouse and the hybridization four generations seed BC1F4 of first backcross generation, cell 3 square metres of area, 100 plants of final singling select that 10 plants of plant types are compact, petiole short handle angle is small and growing way is healthy and strong the neat mouth phase out of 2F1 Individual plant is stand-by, and remaining is pulled out, selected in the neat mouth phase from BC1F4 10 plants of plant types are particularly compact, petiole is especially short, leaf a kind of sedge color oil The healthy and strong individual plant of green, blade subcircular and growing way is stand-by, and remaining is pulled out, in the florescence in April using the individual plant that 2F1 chooses for female parent and with The individual plant that BC1F4 chooses hybridizes for male parent producing Crossbred, and seed maturity harvests respectively, and each hybridization takes about 50 seeds uniform Mix Unified number and obtain first-filial generation seed 3F1.

2 months 2011 in the live first-filial generation seed 3F1 of greenhouse, 9 square metres of plot area, 300 plants of final singling, the neat mouth phase from Middle selection plant type is compact, petiole is smaller compared with short handle angle, leaf nearly circle, the individual plant 20 that leaf a kind of sedge color is glossy dark green and growth is normal and healthy and strong Strain remaining pulls out, and the early flowering season puts fly net, and October, full-bloom stage was pollinated 2 times every 2 days individual plant difference indirect labors, seed maturity Harvest numbering respectively respectively afterwards and obtain second filial generation seed 3F2.

In August, 2011 broadcasts alone a cell, cell face in the live second filial generation seed 3F2 of greenhouse, each individual plant offspring 3 square metres of product, 100 plants of final singling, neat mouth phase find that wherein 1 cell plant shows as when particularly compact plant type, first flower lateral bud not Sprout, in petiole it is short, petiole handle angle is minimum, upright blade, leaf subcircular, leaf a kind of sedge color is glossy dark green, a kind of sedge is sturdy and growth is normal Healthy and strong plant, therefrom preferably 20 plants of individual plants reserved seed for planting in florescence set net, therefrom preferably 10 plants of preferable individual plants of knot, harvesting point respectively Hybridization three generations's seed 3F3 Bian Hao not obtained.

A cell, cell face are broadcast alone in the live hybridization three generations's seed 3F3 of greenhouse, each individual plant offspring within 2 months 2012 3 square metres of product, 100 plants of final singling, the neat each cell outward appearance difference unobvious of mouth phase observation, condition is totally strengthened further according to plant and is eaten Quality, emphasis select and remain 5 cells, and each cell is selected and remain 10 plants of individual plants the most excellent and is listed again, puts fly net, then root respectively According to the general performance of picking time knot etc., 2 cells, each 2 single-strain seed difference of cell focal selection are preferentially selected and remain Numbering obtains four generation seed 3F4 of hybridization respectively for harvesting.

In August, 2012 broadcasts alone a cell, plot area in greenhouse four generations seed 3F4 of live hybridization, 4 individual plant offsprings 3 square metres, 100 plants of final singling, the neat mouth phase observes cell difference unobvious, and each cell selects and remain 10 plants of individual plants the most excellent, its It is remaining to pull out, fly net is put respectively, each cell is selected and remain 2 plants of excellent individual plants of knots performance in the seed collection phase and harvested respectively, Each individual plant takes about 100, seed is well mixed to obtain five generation seed 3F5 of hybridization respectively.Commented in addition, carrying out field planting experiment Germplasm character to be measured and stability are estimated, respectively in July and sowing in October four generation seed 3F4 of hybridization and the upright compact of traditional tender flower stalk Germ plasm resource is transplanted, 10 square metres of cultivated area, seeding row spacing is 20 lis as control using 128 hole plate seedling growths, during 4 leaf of seedling age Rice × 25 centimetres, 200 plants are planted, when picking time is entered, field individual plant regularity is general, and observation trial test is carried out to these individual plants And front and rear contrast, the results showed that upright compact plant character is very stable, and lateral bud is not also sprouted, and mouthfeel is also fine, required Optimality Shape gathers in same germplasm materials, has tentatively formulated tender flower stalk not affected by environment without the upright compact germplasm money of lateral bud Source, now gene homozygosity is general, can further be selfed homozygosis as needed.

2 months 2013 in greenhouse five generation seed 3F5 of live hybridization, 3 square metres of plot area, 100 plants of final singling, neat mouth phase choosing 20 plants of individual plants the most excellent are stayed, remaining is pulled out, and puts fly net respectively, and each cell selects and remain 10 plants of knots in the seed collection phase Show excellent individual plant to harvest respectively, each individual plant takes about 50, seed is well mixed to obtain six generation seed 3F6 of hybridization respectively.

In August, 2013 is in greenhouse six generation seed 3F6 of live hybridization, 3 square metres of plot area, 100 plants of final singling, neat mouth phase choosing 20 plants of individual plants the most excellent are stayed, remaining is pulled out, and puts fly net respectively, and each cell selects and remain 10 plants of knots in the seed collection phase Show excellent individual plant to harvest respectively, each individual plant takes about 50, seed is well mixed to obtain seven generation seed 3F7 of hybridization respectively.Separately Outside, field planting test assessment germplasm character to be measured and stability are carried out, respectively in six generation seeds of July and sowing hybridization in October 3F6, transplanted using 128 hole plate seedling growths, during 4 leaf of seedling age, 10 square metres of cultivated area, seeding row spacing is 25 centimetres of 20 cm x, kind 200 plants are planted, when picking time is entered, field individual plant regularity is preferable, these individual plants is carried out to contrast before and after observation is tasted simultaneously, knot Fruit shows that upright compact plant character is very stable, and lateral bud is not also sprouted, and mouthfeel is also fine, and required merit gathers same In individual germplasm materials, it is successful on the whole and has formulated the upright compact germ plasm resource of tender flower stalk not affected by environment, now gene pure Degree is preferable, can further be selfed homozygosis as needed.

2 months 2014 in greenhouse seven generation seed 3F7 of live hybridization, 3 square metres of plot area, 100 plants of final singling, neat mouth phase choosing 20 plants of individual plants the most excellent are stayed, remaining is pulled out, and puts fly net respectively, and each cell selects and remain 10 plants of knots in the seed collection phase Show excellent individual plant to harvest respectively, each individual plant takes about 50, seed is well mixed to obtain eight generation seed 3F8 of hybridization respectively.This When seed purity it is very high, no upright compact germplasm of lateral bud has gathered more merits.

In August, 2014 is in greenhouse eight generation seed 3F8 of live hybridization, 3 square metres of plot area, 100 plants of final singling, neat mouth phase choosing 20 plants of individual plants the most excellent are stayed, remaining is pulled out, and puts fly net respectively, and each cell selects and remain 10 plants of knots in the seed collection phase Show excellent individual plant to harvest respectively, each individual plant takes about 50, seed is well mixed to obtain nine generation seed 3F9 of hybridization respectively.Separately Outside, field planting test assessment germplasm character to be measured and stability are carried out, respectively in eight generation seeds of July and sowing hybridization in October 3F8 and the upright compact germ plasm resource of traditional tender flower stalk（Control）, transplanted using 128 hole plate seedling growths, during 4 leaf of seedling age, cultivated area 10 square metres, seeding row spacing is 25 centimetres of 20 cm x, plants 200 plants, during into picking time, germplasm field individual plant regularity to be measured Very well, 20 plants of representative individual plants are selected to carry out examining seedling respectively.1 is shown in Table, plant height, strain width and maximum leaf leaf with reference to front and rear date of seeding Handle and the angle data variation of scape, it is seen that germplasm to be measured compares upright Compactness for conventional upright compact germ plasm resource Change degree of convergence very little, germplasm to be measured lateral bud in front and rear date of seeding tender flower stalk first flower are not sprouted, and compare the change of lateral bud germination rate Degree of convergence is up to 485.2%, it is seen that germplasm to be measured is not highly stable affected by environment, and petiole length changes degree of convergence also very little, explanation Exterior quality is very stable.Sampled more people taste suitable Suzhou consumption demand in good taste, and required merit gathers same In one germplasm materials, tested with reference to the field tests of above 2 years, successfully formulated tender flower stalk not affected by environment without side Bud uprightly gathers type germ plasm resource, and gene homozygosity is very high, sees accompanying drawing 2 ~ 4.

Table 1 2014 year summer and autumn germplasm Some Agronomic Traits summary sheet to be measured

The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art Scholar can understand present disclosure and implement according to this, and it is not intended to limit the scope of the present invention.It is all according to the present invention The equivalent change or modification that Spirit Essence is made, it should all be included within the scope of the present invention.

Claims (2)

1. A kind of 1. method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment, it is characterised in that：Select Guangdong Cabbage heart conventional variety Lubao 60 tian, SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2 and SUZHOUQING(sic) type tender flower stalk self-mating system G11 is as parent material, including at least the following steps：

   The first step, using the Guangdong cabbage heart conventional variety Lubao 60 tian as maternal and described SUZHOUQING(sic) type common Chinese cabbage from Friendship is that Q2 is paternal hybrid, obtains first-filial generation seed 1F1；

   Second step, it is male parent backcrossing by recurrent parent and first-filial generation 1F1 of the Lubao 60 tian, obtains first backcross generation First-filial generation seed BC1F1；

   3rd step, the second filial generation seed BC1F2 of first backcross generation is obtained with the first-filial generation BC1F1 selfings of first backcross generation；

   4th step, the compact plant selfing of plant type is selected to obtain the miscellaneous of first backcross generation in the second filial generation BC1F2 of first backcross generation Hand over three generations's seed BC1F3；

   5th step, first-filial generation is obtained with hybridization three generations BC1F3 hybridization using the SUZHOUQING(sic) type tender flower stalk self-mating system G11 to be maternal Seed 2F1, the hybridization three generations BC1F3 selfings of first backcross generation obtain the hybridization four generations seed BC1F4 of first backcross generation；

   6th step, new first-filial generation kind is obtained with the hybridization four generations BC1F4 of first backcross generation hybridization using first-filial generation 2F1 to be maternal Sub- 3F1；

   7th step, the compact plant selfing of plant type is selected to obtain second filial generation seed 3F2 in first-filial generation 3F1；

   8th step, selected in second filial generation 3F2 plant type is compact, upright blade, first flower when lateral bud do not sprout and a kind of sedge color is glossy dark green Plant selfing to obtain hybridization three generations seed 3F3, hybridization three generations's seed 3F3 be that tender flower stalk not affected by environment is uprightly compact Type germ plasm resource；

   The morphological feature of the upright compact germ plasm resource of tender flower stalk is that plant type is compact, upright blade, and lateral bud is not sprouted during first flower, Plant height is 36 ~ 38 centimetres, and strain width is 19 ~ 21 centimetres, the glossy dark green wax powder-free of a kind of sedge color, main a height of 23 ~ 25 centimetres of a kind of sedge, main a kind of sedge slightly for 1.5 ~ 2.5 centimetre, tender flower stalk weighs 59 ~ 62 grams；Blade grows 24.5 ~ 26 centimetres, and blade is wide 14 ~ 16 centimetres, and petiole grows 5.5 ~ 7 centimetres, blade Green glossy subcircular, a kind of sedge leaf petiole and a kind of sedge angle are small, and blade face is open and flat small with a kind of sedge angle；

   The morphological feature of the SUZHOUQING(sic) type common Chinese cabbage self-mating system Q2 is 17 ~ 18 centimetres of plant height, and 24 ~ 25 centimetres of strain is single 278 ~ 283 grams of plant weight, the number of blade are 20 ~ 22, and property of girdling the waist is good, blade subcircular, petiole cochlear, dark green leaf, and petiole is green White；During bolting, a kind of sedge green has draped over one's shoulders wax powder, and tender flower stalk plant type compact half is upright, and petiole and internode are away from short, a kind of sedge leaf petiole and a kind of sedge angle Medium, blade face is open and flat medium with a kind of sedge angle；

   The morphological feature of the SUZHOUQING(sic) type tender flower stalk self-mating system G11 is that plant type is compact, 32 ~ 34 centimetres of plant height, 23 ~ 25 lis of strain Rice；There is wax powder on a kind of sedge green surface, and tender flower stalk weighs 49 ~ 51 grams, and thick 1.5 ~ 2.2 centimetres of a kind of sedge, a kind of sedge is high 21 ~ 22 centimetres；Leaf color is green, and blade is near Circle, blade grow 15 ~ 16 centimetres, and blade is wide 9 ~ 11 centimetres, and petiole grows 5 ~ 6 centimetres, and petiole is wide 1.5 ~ 2.0 centimetres, a kind of sedge leaf petiole Small with a kind of sedge angle, blade face is open and flat big with a kind of sedge angle.
2. 2. the method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment according to claim 1, it is special Sign is：The morphological feature of the Guangdong cabbage heart conventional variety Lubao 60 tian is 26 ~ 28 centimetres of plant height, 28 ~ 34 lis of strain Rice, base leaf blade oval, bottle green, blade grow 17 ~ 19 centimetres, and blade is wide 7.5 ~ 9 centimetres, 5 ~ 6.5 centimetres of petiole length, wide 0.9 ~ 1.5 centimetre；A kind of sedge leaf is narrow avette, and tender flower stalk consolidation bar is even, and dark green glossy, main a kind of sedge is high 22 ~ 26 centimetres, and main a kind of sedge transverse diameter 1.5 ~ 1.8 centimetres, tender flower stalk weighs 35 ~ 40 grams.