CN107347629B - A kind of method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation - Google Patents

A kind of method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation Download PDF

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CN107347629B
CN107347629B CN201710619074.7A CN201710619074A CN107347629B CN 107347629 B CN107347629 B CN 107347629B CN 201710619074 A CN201710619074 A CN 201710619074A CN 107347629 B CN107347629 B CN 107347629B
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flower stalk
tender flower
youlv
sedge
germ plasm
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CN107347629A (en
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蒋明敏
何玲莉
佘旭东
朱建明
李军
刘凤军
张国芹
牟建梅
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Suzhou Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A kind of method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation, it is characterized in that selecting tender flower stalk kind " Youlv 701 cabbage heart " and turnip kind " white jade " as parent material, include the following steps: to take turnip kind " white jade " as maternal and tender flower stalk kind " Youlv 701 cabbage heart " to obtain first-filial generation seed F1 for paternal hybrid;It is female parent with first-filial generation F1, with tender flower stalk kind " Youlv 701 cabbage heart " for paternal hybrid, obtains first backcross generation seed BC1F1;Basal internode distance elongation obvious morning, and the healthy and strong plant BC1F5 of the compact upright blade of plant type are selected after 4 generations again from first backcross generation BC1F1;BC1F5 group is expanded into the tender flower stalk germ plasm resource that numerous acquisition is suitble to disposable harvest operation.Method for creating of the invention is easy to operate, and the yield that the tender flower stalk germ plasm resource of initiative disposably harvests is higher than the yield of itself conventional multi harvest, realizes the target of the disposable harvest operation not underproduction of tender flower stalk.

Description

A kind of method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation
Technical field
The present invention relates to vegetable breeding fields, and in particular to a kind of tender flower stalk germ plasm resource for being suitble to disposable harvest operation Method for creating, the tender flower stalk germ plasm resource can carry out disposable harvest operation mechanically or manually when just entering the early flowering season.
Background technique
Recently as the continuous improvement of labor cost, the cost to plant vegetables is higher and higher, is substituted using machinery artificial The overall process for completing vegetables production is one of important trend of scientific research and innovation.Tender flower stalk is a kind of whole machine of most possible realization One of the vegetable species of tool operation, but the character of current kind cannot entirely appropriate mechanical whole operation demand, main bottle Neck is in harvest link.Existing tender flower stalk kind is that breeding obtains under the conditions of artificial Multiple harvests, be according to field single plant at The different of ripe degree successively repeatedly harvest, and yield can be only achieved peak.And prior art machinery is only possible to disposably harvest, and shows And if be clear to it is disposable early receive, population yield is also not up to peak, if evening receives, necessarily has part to bloom and excessively loses quotient Moral character, population yield are also necessarily remarkably decreased, and are difficult to find an equalization point, therefore, if what existing kind disposably harvested Words, the labour cost that yield will necessarily be decreased obviously, and save at this stage are much not enough to loss caused by making up the underproduction, then pass through The remarkable benefit decline that Ji benefit can be harvested more manually, cannot achieve tender flower stalk entire mechanization operation from economic level.In addition, from Disposable harvesting approach is much higher than traditional Multiple harvests mode for improving tender flower stalk production efficiency and vegetable plot utilization efficiency angle, It is also to be of great significance for cost-saving synergistic particularly with the disposable harvest of realization for large-scale production.
In summary, it is necessary to solve existing tender flower stalk kind and disposably harvest asking of comparing that Multiple harvests yield is decreased obviously Topic, and yet there are no the relevant technologies to solve the problems, such as this.
Summary of the invention
The present invention provides a kind of technological approaches in order to solve the above problem, has invented and a kind of has been suitble to disposable harvest operation The method for creating of tender flower stalk germ plasm resource, obtained material not only can be used as the donor parents of new traits, can also be as kind Directly utilize.
In order to achieve the above objectives, the technical solution adopted by the present invention is that: a kind of tender flower stalk kind being suitble to disposable harvest operation The method for creating of matter resource selects tender flower stalk kind " Youlv 701 cabbage heart " and turnip kind " white jade " as parent material, at least wraps Include the following steps:
It is that male parent is miscellaneous that the first step, which is maternal and the tender flower stalk kind " Youlv 701 cabbage heart " with the turnip kind " white jade ", It hands over, obtains first-filial generation seed F1;
Second step is female parent with the first-filial generation F1, with the tender flower stalk kind " Youlv 701 cabbage heart " for paternal hybrid, Obtain first backcross generation seed BC1F1;
Third step selects basal internode after 4 generations since the first backcross generation seed BC1F1 using single plant method Distance elongation obviously earlier than the tender flower stalk kind " Youlv 701 cabbage heart " and the healthy and strong plant of the compact upright blade of plant type, obtains Five generation strain seed BC1F5;
The five generations strain seed BC1F5 group is expanded numerous, therefrom to select 1 growing way, uniformity best strain by the 4th step System, is as suitble to the tender flower stalk germ plasm resource (JT-1) of disposable harvest operation.
The tender flower stalk germ plasm resource (JT-1) belongs to ripe type in cabbage heart, and the flower stalk yield disposably harvested is compared itself and passed The flower stalk yield of system Multiple harvests significantly increases production 10.2~14.5%, also compares parent's " Youlv 701 cabbage heart " conventional multi harvest Flower stalk yield significantly increases production 7.5~11.6%, and the most of single a kind of sedges disposably harvested are without little Hua, and commodity is on the whole also Tradition is substantially better than with " neat mouth flower " as single a kind of sedge of standard Multiple harvests;The compact upright blade of tender flower stalk germ plasm resource plant type, The elongation of basal internode distance is obviously earlier than parent " Youlv 701 cabbage heart " equivalent type traditional category, plant height under same cultivation condition It is apparently higher than parent " Youlv 701 cabbage heart " equivalent type traditional category;Tender flower stalk germ plasm resource strain when conventional multi harvests 44.1 centimetres high, 27.5 centimetres of strain spoke, main a kind of sedge is 20.0 centimetres long, and thick 1.68 centimetres of a kind of sedge, 44.5 grams of single a kind of sedge quality;The tender flower stalk kind Matter resource is in disposable harvest 37.4~40.7 centimetres of plant height, and 20.3~24.8 centimetres of strain spoke, main a kind of sedge is 22.7~23.8 lis long Rice, thick 1.75~1.86 centimetres of a kind of sedge, 49.9~51.5 grams of single a kind of sedge quality.
Related content in above-mentioned technical proposal is explained as follows:
1, in above scheme, the tender flower stalk germ plasm resource belongs to Guangdong cabbage heart type, belongs to cruciate flower on botany classification Section Brassica genus Vegetable Crops of Brassica pakchoi subspecies tender flower stalk mutation indicates to describe readily available " JT-1 " in full.
2, in above scheme, the tender flower stalk kind " Youlv 701 cabbage heart " is Guangzhou agriculture research institute (existing entitled Guangzhou City's academy of agricultural science) breeding, in March, 2005 by Guangdong Province's crop varietal approval committee, authorization is numbered: Dish 2005002 is examined in Guangdong.Its feature is that growth potential is strong, and plant shape is shorter strong, 30.4 centimetres of plant height, 26.7 centimetres of strain;Ji Ye Slightly wicker leaf shape, a kind of sedge leaf wicker leaf shape, a kind of sedge leaf is few, and section is dredged, and tender flower stalk consolidation is well-balanced, is not easy hollow, storage tolerance, glossy dark green glossy, main A kind of sedge height 23~25 centimetres of (length), transverse diameter l.5~2 centimetre, 45~50 grams of single a kind of sedge quality, in ripe, sowing to just receipts 37~43 It, continues harvesting 7~l0 days, and bolting is neat, and commodity Comprehensive Traits are good, good quality, virus resistance disease, downy mildew, wide adaptability, Resistance is strong, high and stable yield, every per mu yield l000~l500kg, is suitable for outlet.The kind belongs to Guangdong cabbage heart type, is planting Belong to Cruciferae Brassica genus Vegetable Crops of Brassica pakchoi subspecies tender flower stalk mutation in object credit class.Seed is ground purchased from Guangzhou agricultural sciences Study carefully institute (Guangzhou Gan Nong development in agricultural science and technology Co., Ltd).
3, in above scheme, the turnip kind " white jade " be from Japan introduce Early-medium maturing variety, fleshy root milky, Subcircular, meat are close, evening occurs for cellulosic, blade is compact, strong green, it is glossy, incise few, be generally used for eating or doing sand raw It draws.Belong to Cruciferae Brassica genus Vegetable Crops of Brassica turnip subspecies on botany classification.Seed is purchased from the green east agricultural technology in Beijing Research institute.
4, in above scheme, it is suitble to the biography of tender flower stalk germ plasm resource (JT-1) and Youlv 701 cabbage heart of disposable harvest operation It unites the standards of Multiple harvests: when main colored a kind of sedge height is flushed with siphonal lobe, when overall bud is intended to out and does not open, often bursting forth with several small Flower is commonly called as " neat mouth flower ", is Harvesting Date, harvests several times;Too early, flower a kind of sedge is excessively tender for harvesting, influences yield;Harvest too late, dish A kind of sedge is excessively high, and fiber increases, and quality is then poor, yield decline.
There are two types of the standards disposably harvested for being suitble to the tender flower stalk germ plasm resource (JT-1) of disposable harvest operation: existing respectively JT-1, Youlv 701 cabbage heart group in first single plant reach " neat mouth flower " standard same day and disposably all harvest JT-1.Oil The standard of green 701 cabbage heart disposably harvested: first single plant reaches " neat mouth flower " the standard same day one in Youlv 701 cabbage heart group Secondary property all harvests.
5, in above scheme, germplasm, which refers to, determines biological heredity character, and its hereditary information is transmitted to offspring's from parental generation Inhereditary material is the entity of objective reality, and gene is known as on science of heredity.There is utility value in heredity, breeding and production All living things material can be referred to as germ plasm resource, be to cultivate the substance base of new varieties from germ plasm resource for the angle of breeding Plinth, the form of expression may is that local varieties, improve the breed, the kind of new breeding, introduced variety, seed, mutant, wild species, Kindred plant, the various biotypes of artificial creation, vegetative propagation organ, individual cells, individual chromosome, individual gene, very To DNA fragmentation etc..
Design concept of the invention: Multiple harvests mode is compared in order to solve traditional disposable harvesting approach of tender flower stalk kind and is produced The problem of amount significantly reduces, is readily apparent that and field single plant is allowed uniformly to reach harvesting standard, so that it may realize, in fact due to seed Purity can not accomplish consistent completely with factors such as field subenvironments, be difficult to accomplish, it is understood that spending in the part single plant beginning latter Secondary property harvesting yield necessarily declines, this is because evening receipts, which necessarily have plant part to bloom, excessively loses commodity.And only beginning It disposably harvests before spending and single a kind of sedge quality is made to reach peak, disposable harvest is possible to maintain an equal level with the yield of Multiple harvests. But the harvesting before beginning to spend of existing tender flower stalk kind does not reach maximum value due to single a kind of sedge quality, disposable crop also can it is obvious under Drop, therefore, to find a kind of beginning Hua Qiandan a kind of sedge quality can reach the kind of maximum value, and be difficult to screen in current kind It arrives.We are had found unintentionally and present inventive concept one when using turnip improvement tender flower stalk disease resistance trait in the offspring of backcrossing The material of cause has carried out purposive improvement then, has obtained above-mentioned germplasm " JT-1 ", and the yield disposably harvested before spending that begins is high In the yield of its own Multiple harvests, and it is significantly higher than the yield of traditional tender flower stalk kind " Youlv 701 cabbage heart ", and disposable The tender flower stalk commodity of harvest is not more suitable for welcoming colored consumption area.In addition, genetic analysis shows that new traits can stablize something lost It passes, illustrates to can use the donor of " JT-1 " as new traits gene, gather other merits, further increase yield and product Matter establishes the tender flower stalk Breeds that different type is suitble to disposable harvest operation, meets the diversity requirement of production.
Characteristic of the invention is artificially to break subspecies estrangement, so that tender flower stalk and turnip gene are merged into each other, wound A kind of method for creating of the tender flower stalk germ plasm resource of suitable mechanical or artificial disposable harvest operation, method for creating operation are made Simply, a kind of new way solved the problems, such as is provided for tender flower stalk dart transfer synergy production, while has started a tender flower stalk germplasm The new route of innovation enriches tender flower stalk germplasm resource bank.
Tender flower stalk belongs to Cruciferae Brassica genus Vegetable Crops of Brassica pakchoi subspecies tender flower stalk mutation in Plant Taxonomy, special to produce To spend a kind of sedge and a kind of sedge leaf as the vegetables of edible tissue, cabbage heart is wherein one of main Types, and only one province's whole year sown area of Guangdong is not It is one of China's main vegetables less than 250,000 hectares.It is suitble to machinery using the tender flower stalk germ plasm resource that method of the invention is formulated out Or artificial disposable harvest, can at least save 300~400 yuan of labour costs per acre, increase newly per acre scale and benefit at least 200~ It 300 yuan, can cost-saving synergistic 4~500,000,000 yuan if being calculated with 1,000,000 mu of application, it is seen that application prospect of the invention will be very wide It is wealthy, there is significant progress compared with traditional technology.
Detailed description of the invention
Attached drawing 1 is the field tender flower stalk germ plasm resource " JT-1 " photo of the suitable disposable harvest operation of the embodiment of the present invention.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and embodiments:
A kind of embodiment: method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation
In the following description, related parent material is that tender flower stalk kind " Youlv 701 cabbage heart " and turnip kind are " white It is beautiful ".
Academy of Agricultural Sciences, Suzhou City (also known as Agricultural Science Inst. of Taihu District of Jiangsu Prov.) started a kind of be suitble in 2013 The initiative of the tender flower stalk germ plasm resource of disposable harvest operation, specific implementation step are as follows:
At the beginning of 1 month 2013 in greenhouse be broadcast live turnip kind " white jade turnip ", therefrom select the single plant of 5 plants of robust growths as Female parent, greenhouse live streaming tender flower stalk kind " Youlv 701 cabbage heart ", therefrom selects the single plant of 5 plants of robust growths as father at the beginning of 2 months the same years This, florescence hybridization, mixing harvesting obtains first-filial generation seed F1 after seed is mature.
First-filial generation seed F1 and tender flower stalk kind " Youlv 701 cabbage heart " is broadcast live in greenhouse in July, 2013, and the florescence respectively selects 5 Strain robust growth single plant is female parent with first-filial generation F1, and tender flower stalk kind " Youlv 701 cabbage heart " is male parent backcrossing, and seed single plant is adopted Mixed in equal amounts obtains first backcross generation seed BC1F1 after receipts.
First backcross generation seed BC1F1 is broadcast live in greenhouse within 2 months 2014, more than 100 strain of final singling therefrom selects 10 plants of shapes and parent This " Youlv 701 cabbage heart " single plant that appearance is similar and growing way is healthy and strong is selfed in florescence bagging, mixed in equal amounts after the harvesting of seed single plant Obtain two generation seed BC1F2 of first backcross generation.
In July, 2014 therefrom selects 3 plants of bases in two generation seed BC1F2 of greenhouse live streaming first backcross generation, more than 300 strain of final singling The elongation of portion internode distance is obviously earlier than traditional tender flower stalk kind and upright blade is compact, single plant of the glossy dark green growing way stalwartness of a kind of sedge color is in the florescence Bagging selfing, seed single plant harvest the three generations's seed BC1F3 for obtaining first backcross generation respectively.
The three generations strain seed BC1F3 of 3 first backcross generations, more than 150 strain of final singling, each strain are broadcast live within 2 months 2015 in greenhouse It selects the elongation of 3 plants of basal internodes distance obviously earlier than traditional tender flower stalk kind in system and upright blade is compact, the glossy dark green growing way of a kind of sedge color is strong Strong single plant is selfed in florescence bagging, and seed single plant obtains four generation seed BC1F4 of 9 first backcross generations after harvesting respectively.
The four generation strain seed BC1F4 of 9 first backcross generations are broadcast live in July, 2015 respectively in greenhouse, distinguish more than 60 strain of final singling, 9 strain kind growing ways are neat, select that 5 plants of early flowering seasons are consistent, basal internode distance elongation is obviously earlier than traditional dish in each strain kind A kind of sedge kind and upright blade is compact, single plant of the glossy dark green growing way stalwartness of a kind of sedge color is selfed in florescence bagging, seed single plant harvests obtain respectively Obtain five generation seed BC1F5 of 9 first backcross generations.The same period is for newfound character, that is, basal internode distance elongation obviously earlier than biography The character for tender flower stalk kind of uniting carries out genetic development research.
Five generation seed BC1F5 of 9 first backcross generations, respectively more than 60 strain of final singling are broadcast live within 2 months 2016 in greenhouse, the florescence covers net The bee production of hybrid seeds is put, 9 strains are obtained.The strain for therefrom selecting 1 growing way, uniformity best is numbered " JT-1 " as research object.
Autumn in 2016 carries out field verification test:
2 kinds of test materials, i.e. strain kind " JT-1 " and tender flower stalk kind " Youlv 701 cabbage heart ".
2 kinds of harvesting approaches, i.e., disposable harvest and Multiple harvests, wherein the disposable harvest opportunity of " JT-1 " is divided to two again Kind, reach " neat mouth flower " the harvest standard same day in " Youlv 701 cabbage heart " and " JT-1 " first single plant respectively, in both harvests " JT-1 " is disposably harvested under opportunity.Multiple harvests are consistent with traditional water content in harvest.
5 test process are set altogether, and 30 square metres of plot area, 1000 plants of final singling, the random district's groups arrangement in field is repeated 3 times. Test result shows that " JT-1 " is significantly higher than itself Multiple harvests after planting 40.5 days and the 43.5 days yield disposably harvested Yield, the extremely significant yield less than itself Multiple harvests of the yield that " Youlv 701 cabbage heart " disposably harvests, and " JT-1 " is primary Property harvest yield be all remarkably higher than the yield of " Youlv 701 cabbage heart " Multiple harvests, see Table 1 for details and table 2 for main experimental data.Separately Outside, genetic analysis shows that new traits can stablize heredity, is controlled by cell nucleus gene, illustrates can use " JT-1 " as new The donor of character gene gathers other merits, further increases yield and quality, establishes different type and is suitble to disposable receive The tender flower stalk Breeds for obtaining operation, meet the diversity requirement of production.Conclusion: " JT-1 " is suitable as the dish of disposable harvest operation A kind of sedge germ plasm resource.
" JT-1 " is different from traditional tender flower stalk kind, and the yield disposably harvested can be higher than the original of the yield of Multiple harvests Because being: being shown in Table 1,40.5 days (when i.e. first single plant reaches " neat mouth flower " standard) after traditional category " Youlv 701 cabbage heart " is broadcast The cell average product disposably harvested is 37.33 kilograms, and the cell average product 45.25 far below conventional multi harvest is public Jin.This is because yield is mainly determined by single a kind of sedge quality and harvest item number, harvest item number is almost the same under this experimental condition, produces The difference of amount is mainly determined by single a kind of sedge quality, that is to say, that the high yield of single a kind of sedge quality is also just high.2 are shown in Table, Youlv 701 dish Single a kind of sedge quality that the heart disposably harvests cell is 40.8 grams, far below 47.3 grams of single a kind of sedge quality of conventional multi harvest cell, from A kind of sedge slightly and long two data of a kind of sedge it is also seen that two ways harvest single a kind of sedge quality difference (forefathers research shows that single a kind of sedge quality and A kind of sedge is thick and a kind of sedge length is positively correlated), a kind of sedge disposably harvested is slightly 1.55 centimetres and a length of 19.5 centimetres of a kind of sedge and is respectively less than Multiple harvests Thick 1.71 centimetres and 21.4 centimetres of a kind of sedge, it is seen that a kind of sedges of traditional category Youlv 701 cabbage heart many single plants when disposably harvesting slightly and A kind of sedge length fails to reach maximum value, then, single a kind of sedge quality is impossible to reach peak, and yield will be bright compared to Multiple harvests Aobvious decline.And the yield 48.63 and 50.52 kilogram that " JT-1 " is disposably harvested is significantly higher than the yield of conventional multi harvest 44.12 kilograms of (see Table 1)s, the single a kind of sedge quality 49.9 and 51.5 gram disposably harvested are apparently higher than single a kind of sedge quality of Multiple harvests 44.5 grams of (see Table 2)s, it is opposite with traditional category performance.As can be seen from Table 2, processing 1 i.e. when JT-1 after broadcasting 40.5 days once Property harvest when, plant height reaches 37.4 centimetres, a length of 22.7 centimetres of a kind of sedge of harvest, thick 1.75 centimetres of a kind of sedge, 49.9 grams of single a kind of sedge quality, place For reason 2 i.e. when JT-1 disposable harvest at 43.5 days after broadcasting, plant height reaches 40.7 centimetres, and a length of 23.8 centimetres of a kind of sedge of harvest, a kind of sedge Thick 1.86 centimetres, 51.5 grams of single a kind of sedge quality, processing 3 is i.e. when JT-1 Multiple harvests, and plant height reaches 44.1 centimetres, and a kind of sedge of harvest is long Be 20.0 centimetres, thick 1.68 centimetres of a kind of sedge, 44.5 grams of single a kind of sedge quality, from 3 of above-mentioned JT-1 processing as can be seen that single a kind of sedge quality, A kind of sedge is slightly and the rule that presents of the long size of a kind of sedge is that 2 > of processing handle 1 > and handle 3, and cell production is also presented identical rule, and plant height Rule is presented and handles 2 > processing 1 for 3 > of processing.This and JT-1 are as breeding time, to increase edible a kind of sedge length more next for fiber backward It is shorter, a kind of sedge slightly also just become smaller it is related, in this way handle 3 i.e. conventional multi harvesting approach list a kind of sedge quality be less than the primary of front instead Property harvesting approach, reflect to being also in this way, the yield that " JT-1 " is disposably harvested is higher than its own Multiple harvests on cell production Yield, to be apparently higher than the production disposably harvested different from universal law, that is, Multiple harvests yield of traditional tender flower stalk kind Amount.It there occur a phenomenon in which that being primarily due to " JT-1 " same period plant height is apparently higher than traditional tender flower stalk kind, reaching " neat mouth flower " " JT-1 " it is long can to harvest enough a kind of sedges in the past, guarantees that single a kind of sedge quality reaches peak before beginning to spend, and generates this phenomenon Reason is because " JT-1 " is under normal cultivation condition again, and the elongation of basal internode distance is obviously earlier than oil about since 5 leaf phases The tradition tender flower stalk kind such as green 701 cabbage heart, stem and leaf while fast-growth, bolting is normal, this character leads to " JT-1 " same period plant height It is apparently higher than Youlv 701 cabbage heart equivalent type tradition tender flower stalk kind, i.e., 40.5 days " JT-1 " plant heights reach 37.4 centimetres after broadcasting It is 7.2 centimetres higher than same period Youlv 701 cabbage heart plant height, and plant height when than Youlv 701 cabbage heart Multiple harvests is 3.1 centimetres high; 43.5 days plant heights reach 40.7 centimetres after " JT-1 " is broadcast, and plant height when than Youlv 701 cabbage heart Multiple harvests is 6.4 centimetres high, And plant height when " JT-1 " Multiple harvests reaches 44.1 centimetres, plant height when than Youlv 701 cabbage heart Multiple harvests is 9.8 lis high Rice.
From commodity, most of single a kind of sedge that " JT-1 " is disposably harvested will be significantly better than without little Hua, commodity The tender flower stalk commodity of Multiple harvests, more suitable for not welcoming colored consumption area.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.

Claims (3)

1. a kind of method for creating for the tender flower stalk germ plasm resource for being suitble to disposable harvest operation, it is characterised in that: selection tender flower stalk kind " Youlv 701 cabbage heart " and turnip kind " white jade " are used as parent material, include at least the following steps:
The first step is maternal and the tender flower stalk kind " Youlv 701 cabbage heart " for paternal hybrid with the turnip kind " white jade ", obtains Obtain first-filial generation seed F1;
Second step is female parent with the first-filial generation F1, with the tender flower stalk kind " Youlv 701 cabbage heart " for paternal hybrid, is obtained First backcross generation seed BC1F1;
Third step selects basal internode distance after 4 generations since the first backcross generation seed BC1F1 using single plant method Elongation obviously earlier than the tender flower stalk kind " Youlv 701 cabbage heart " and the healthy and strong plant of the compact upright blade of plant type, obtained for five generations Strain seed BC1F5;
The five generations strain seed BC1F5 group is expanded numerous, therefrom to select 1 growing way, uniformity best strain by the 4th step, As it is suitble to the tender flower stalk germ plasm resource of disposable harvest operation;
The tender flower stalk germ plasm resource belongs to ripe type in cabbage heart, and the flower stalk yield disposably harvested is harvested compared with itself conventional multi Flower stalk yield significantly increases production 10.2~14.5%, and also the flower stalk yield compared with parent's " Youlv 701 cabbage heart " conventional multi harvest significantly increases Produce 7.5~11.6%;The compact upright blade of plant type of the tender flower stalk germ plasm resource, basal internode distance is stretched under same cultivation condition For length obviously earlier than parent " Youlv 701 cabbage heart ", plant height is apparently higher than parent " Youlv 701 cabbage heart ";The tender flower stalk germ plasm resource exists 44.1 centimetres of plant height when conventional multi harvests, 27.5 centimetres of strain spoke, main a kind of sedge is 20.0 centimetres long, and thick 1.68 centimetres of a kind of sedge, single a kind of sedge quality 44.5 grams;The tender flower stalk germ plasm resource is in disposable harvest 37.4~40.7 centimetres of plant height, 20.3~24.8 centimetres of strain spoke, main A kind of sedge is 22.7~23.8 centimetres long, and thick 1.75~1.86 centimetres of a kind of sedge, 49.9~51.5 grams of single a kind of sedge quality.
2. the method for creating of the tender flower stalk germ plasm resource according to claim 1 for being suitble to disposable harvest operation, feature exist In: the tender flower stalk kind " Youlv 701 cabbage heart " is Guangzhou Institute of agricultural sciences breeding, passes through Guangdong Province in March, 2005 Crop varietal approval committee, authorization number: dish 2005002 is examined in Guangdong.
3. the method for creating of the tender flower stalk germ plasm resource according to claim 1 for being suitble to disposable harvest operation, feature exist In: the turnip kind " white jade " is the Early-medium maturing variety introduced from Japan, and fleshy root milky, subcircular, meat are close, fine Tie up matter and evening occur, blade is compact, strong green, it is glossy, incise it is few.
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CN101461308A (en) * 2009-01-03 2009-06-24 孙和平 Method for breeding dual-purpose rape used for oil and vegetable

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