CN109287473A - A kind of application of the diploid watermelon self-mating system of the selection and breeding of diploid watermelon self-mating system - Google Patents
A kind of application of the diploid watermelon self-mating system of the selection and breeding of diploid watermelon self-mating system Download PDFInfo
- Publication number
- CN109287473A CN109287473A CN201811406802.7A CN201811406802A CN109287473A CN 109287473 A CN109287473 A CN 109287473A CN 201811406802 A CN201811406802 A CN 201811406802A CN 109287473 A CN109287473 A CN 109287473A
- Authority
- CN
- China
- Prior art keywords
- watermelon
- disease
- fruit
- self
- mating system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of selection of diploid watermelon self-mating system and applications, are related to field of plant variety breeding technology.The selection of the diploid watermelon self-mating system, comprising: 1) screen disease-resistant single plant in greenhouse sick nursery and carry out continuous three generations artificial autocopulation pollination and stay fruit, obtain self-mating system Sugarlee-3；2) it is female parent with Sugarlee-3, carries out sexual hybridization as male parent easily to bear fruit variety of watermelon, obtain recombination system WF2201；3) recombination system WF2201 progress artificial autocopulation is stayed into fruit, obtaining F2 generation is isolated from the recombinant and is；4) in obtained F2 generation, is isolated from the recombinant the disease-resistant single plant in being and is continuously selfed and stay fruit, obtain F6 for disease-resistant high-quality from presentate melon system；5) in obtained F6 generation, is subjected to expansion breeding, obtains diploid watermelon self-mating system.The diploid watermelon self-mating system that the present invention cultivates is suitable as cultivating the parent of new water melon breed, can give its disease-resistant characteristic hereditary to its first-filial generation.
Description
Technical field
The invention belongs to field of plant variety breeding technology, and in particular to a kind of selection of diploid watermelon self-mating system and choosing
The application for the diploid watermelon self-mating system educated.
Background technique
Watermelon is one of big fruit in the world ten, even more Chinese Important Economic crop.Watermelon blight is to influence countries in the world
The important disease of watermelon production, is by Fusarium oxysporum f. sp. niveum (Fusarium oxyporum f.sp.niveum)
Soil-borne disease caused by root is infected, continuous crop aggravates the generation and propagation of the disease.Currently, withered for watermelon in the world
Disease, which there is no, effectively prevents medicament, although grafting cultivation can mitigate the generation of the disease, grafting cultivation not only reduces watermelon
Taste quality, while considerably increasing production cost, breeding anti-blight variety of watermelon is the generation for mitigating the disease, reduces
Production cost ensures the high-quality most economical effective approach of product safety.
Watermelon is the extremely strong industrial crops of commodity, and consumer's height focuses on quality, and (i.e. the color of pulp, soluble sugar contain
Amount and the mouthfeel delicacy degree of pulp etc.), and what the producer focused on is that disease resistance and yield (resist one or more diseases, are easy to sit
Fruit, fruit be big, resistance to storage and transportation), both solve the new water melon breed that contradictory most effective approach is exactly breeding High quality and diseases resistance high yield.It grinds
Study carefully discovery, there is negative correlation between the disease resistance and quality of watermelon, (pol is low, meat is thick, mouthfeel for disease-resistant variety mostly poor quality
Firmly).So the anti-blight variety of watermelon quality applied in domestic and international watermelon production is all poor, famous such as U.S.'s incubation resists
Wilt disease variety of watermelon: Charleston Gray, Jubilee, Crimson Swet, Calhoun Gray, Smokylee,
Summit, Dixilee, Sugarlee etc., performance pulp soluble sugar content is relatively low, pulp is more, meat is hard, cannot
Meet modern consumer to the high standard demand of quality of watermelon.And these kinds all show and late-maturing, difficult bear fruit, is not low temperature resistant weak
Light, is not suitable for facility cultivation, and China overwhelming majority area is all difficult to plant.
Last decade, China's facility cultivation watermelon area expand rapidly, and it is early good (south that facility cultivation watermelon, which produces main breed,
Side area) and capital it is glad No. two (northern areas), the outstanding advantages of the two kinds be high-quality (pulp soluble sugar content height,
That meat is crisp is delicate, taste quality is good), easily bear fruit under low temperature and poor light, fruit development period it is short (less than 30 days), significant deficiency is not
Disease-resistant (especially not anti-blight), easy dehiscent fruit, the flesh that easily falls.The disease-resistant watermelon variety ' western agriculture eight ' that China is independently bred as it is main
Advantage is that fruit is big, yield is high, resistance to storage and transportation, and major defect is inferior quality (pulp is more, mouthfeel is partially hard, weak flavor), and
Not tolerance to low temperature and weak light is not suitable for facility cultivation.
Currently, all kinds of variety of watermelon (disease-resistant variety, the not disease-resistant variety, middle large fruit product of China's watermelon commodity production
Kind and small fruit type kind) it is all Diploid hybrid, derive from the sexual hybridization of two diploid watermelon self-mating systems.Therefore,
Diploid watermelon self-mating system is to cultivate the important parental source of diploid watermelon hybrid new breed.But there is presently no one kind
Can be by the selection of watermelon disease resistance trait and good quality and high output character transformation simultaneously to a diploid watermelon self-mating system, and incite somebody to action
Its excellent character is complete or at least most of stabilization is hereditary to its commodity cenospecies.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of selection of diploid watermelon self-mating system and application, it is full
Demand of the sufficient variety of watermelon to quality, disease resistance and yield, while solving to the disease-resistant watermelon inbred lines plant of high-quality and kind
The demand of son；It is also used as High quality and diseases resistance Watermelon Parent, the business for cultivating different fruit type and rind appearance feature is high
Quality disease-resistant high yield diploid hybrid new water melon breed.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of selections of diploid watermelon self-mating system, comprising the following steps:
1) anti-blight Watermelon Parent nursery is colonized in watermelon blight greenhouse sick nursery, the disease-resistant single plant screened
Continuous three generations artificial autocopulation is carried out under greenhouse sick nursery cultivation condition again and stays fruit, obtains disease-resistant self-mating system Sugarlee-3；
2) it is female parent with the self-mating system Sugarlee-3, is carried out using high-quality variety of watermelon of easily bearing fruit as male parent sexual miscellaneous
It hands over, obtains watermelon composite-crossing recombination system WF2201；
3) watermelon composite-crossing recombination system WF2201 progress artificial autocopulation is stayed into fruit, obtaining F2 generation is isolated from the recombinant and is；
4) being isolated from the recombinant the F2 generation is plantation in watermelon blight greenhouse sick nursery, and screening obtains disease-resistant single plant and goes forward side by side
The continuous four generations artificial autocopulation of row stays fruit, obtains F6 generation single melon system；
5) the F6 generation single melon system plantation is screened into disease resistance and Agronomic in watermelon blight greenhouse sick nursery
Consistent list melon system is diploid watermelon self-mating system.
Preferably, step 1), step 4) and step 5) it is described it is disease-resistant include: that seedling stage is disease-resistant and Adult plant is disease-resistant.
Preferably, step 1) the anti-blight Watermelon Parent is ' Sugarlee '.
Preferably, step 2) the high-quality variety of watermelon of easily bearing fruit is ' early good '.
Preferably, the step 5) economical character includes: that fruit is high round, and dark green sharp mouth item is covered at the green bottom of pericarp, and pericarp is thick
< 1.2cm, center sugar > 12.5%, side sugar > 8.5%, meat is crisp, fiber is few.
The diploid watermelon self-mating system that is obtained the present invention also provides above-mentioned selection breeding and its in breeding watermelon quotient
Application in kind.
Preferably, using the diploid watermelon self-mating system as male parent.
Preferably, the male parent and maternal planting number ratio are 1:15.
Preferably, the male parent implantation time is earlier than 7~10d of female parent.
Preferably, all lateral bines of female parent are extractd, only a piece main stem of reservation, and by male flowers all in main stem and male flower
Flower bud is all extractd.
The present invention provides a kind of selections of diploid watermelon self-mating system, the choosing with conventional diploid watermelon self-mating system
It educates method to compare, meets the demand of the quality to variety of watermelon, disease resistance and yield, while solving to the disease-resistant west of high-quality
The demand of melon self-mating system Plants and Seeds；It is also used as High quality and diseases resistance Watermelon Parent, for cultivating outside different fruit type and pericarp
See the business high-quality disease-resistant high yield diploid hybrid new water melon breed of feature.
The present invention also provides application of the above-mentioned diploid watermelon self-mating system in breeding commodity variety of watermelon, with described two
Times body watermelon inbred lines are male parent, are female parent with other diploid watermelon self-mating systems, carry out sexual hybridization, that is, had
The first-filial generation new water melon breed of high-quality anti-blight hereditary capacity.First-filial generation new water melon breed obtained with it is similar general
Logical variety of watermelon compares, quality and flavor and anti-watermelon blight and high yield characteristics with high standard, and the resistance to storage and transportation of fruit, no
The easy dehiscent fruit of falling flesh, therefore the common approval by consumers and producers.
Detailed description of the invention
Fig. 1 is the flow chart of breeding diploid watermelon self-mating system of the present invention.
Specific embodiment
The present invention provides a kind of selections of diploid watermelon self-mating system, comprising the following steps:
1) anti-blight Parent Seedling is colonized in watermelon blight greenhouse sick nursery, the disease-resistant single plant screened carries out
Fruit is stayed in continuous three generations's selfing, obtains self-mating system ' Sugarlee-3 '；
2) it is female parent with the self-mating system ' Sugarlee-3 ', is carried out using high-quality variety of watermelon of easily bearing fruit as male parent sexual miscellaneous
It hands over, obtains watermelon composite-crossing recombination system WF2201；
3) the watermelon composite-crossing recombination system WF2201 artificial autocopulation is stayed into fruit, obtaining F2 generation is isolated from the recombinant and is；
4) being isolated from the recombinant the F2 generation is plantation in watermelon blight greenhouse sick nursery, screens disease-resistant single plant and is connected
Continuous four generations orientation self cross and selection stays fruit, obtains F6 generation single melon system；
5) the F6 generation single melon system plantation is screened into disease resistance and Agronomic in watermelon blight greenhouse sick nursery
Consistent list melon system is diploid watermelon self-mating system.
In the selection of diploid watermelon self-mating system of the present invention, as shown in Fig. 1, by anti-blight parent's nursery
It is colonized in watermelon blight greenhouse sick nursery, screens disease-resistant single plant and carry out continuous three generations's selfing and stay fruit, obtain self-mating system
‘Sugarlee-3'.Anti-blight parent of the present invention is preferably ' Sugarlee '.The present invention is to ' Sugarlee '
The sub or slave national germplasm of routine ' Sugarlee ' kind saved is bred preferably by those skilled in the art there is no particular determination in source
Resources bank obtains.The method for building up of watermelon blight greenhouse sick nursery of the present invention, preferably includes: selection high-land and flatly
Block builds area in 750~1500m2The facility of scale links greenhouse (3~5 beam-connected greenhouse), and installs 1 meter in greenhouse surrounding
Deep antiseep film.The highly pathogenic of Races of F. oxysporum f. sp. niveum 1 is inoculated in plastic shed soil using wheat inocalation method
Bacterial strain, and susceptible variety of watermelon ' early good ' is cultivated within continuous 2 years, withered germ of water-melon is pathogenic in reinforcing plastic shed soil, builds up
Pathogenic strong and stable watermelon blight greenhouse Disease garden identification base.Disease resistance of the present invention preferably includes: seedling stage is disease-resistant
It is disease-resistant with Adult plant.During fruit is stayed in continuous three generations selfing of the present invention, except screening seedling stage and Adult plant show it is disease-resistant
Outside single plant, it is also preferable to include the normal fruits of fructescence harvesting growth and development to carry out species test identification, specifically includes: center sugar
Greater than 11%, fruit section flesh color uniformly, without hollow white muscle.
After obtaining self-mating system ' Sugarlee-3 ', the present invention is female parent with ' Sugarlee-3 ', with high-quality watermelon of easily bearing fruit
Kind is that male parent carries out sexual hybridization, obtains watermelon composite-crossing recombination system WF2201.High-quality watermelon of easily bearing fruit of the present invention
Kind is preferably early good.The present invention is before carrying out the sexual hybridization, and preferably ' Sugarlee-3 ' plantation by described in is in described big
Canopy sick nursery, by other diploid watermelon kinds ' early good (8424) ', plantation is in conventional greenhouse, when blooming, with
Seedling stage and Adult plant show disease-resistant single plant as female parent in ' Sugarlee-3 ', are that male parent progress is sexual with ' early good (8424) '
Hybridization.There is no particular determinations, preferably conventional commercial in source of the present invention to other diploid watermelon kinds ' early good '
Product seed.
After obtaining watermelon composite-crossing recombination system WF2201, the composite-crossing recombination system WF2201 that the present invention will obtain
Artificial autocopulation stays fruit, and acquisition F2 generation, which is isolated from the recombinant, is.Composite-crossing recombination system artificial autocopulation of the present invention preferably exists when staying fruit
Hainan is bypassed the immediate leadership plus generation, is preferably included within the selfing that is manually isolated that florescence carries out strictly and stays fruit, the maturity period harvests WF2201's
It reserves seed for planting from presentate melon, i.e., acquisition F2 generation, which is isolated from the recombinant, is.
It obtains after F2 generation is isolated from the recombinant and is, it is plantation in watermelon blight that obtained F2 generation is isolated from the recombinant by the present invention
In greenhouse sick nursery, screens disease-resistant single plant and carry out continuous four generations artificial autocopulation pollination and stay fruit, obtain F6 generation single melon system.In the present invention
Using the greenhouse wilt disease Disease garden identification base built up, being isolated from the recombinant to the F2 generation of initiative is that progress seedling stage and Adult plant are dual anti-
The continuously-directional selfing system of characteristic of disease selects, in an embodiment of the present invention, by the F2 of the WF2201 of the numerous acquisition in south for heavy constituent
It plants in greenhouse sick nursery from the single melon seed seedling-raising for being, sows in spring under greenhouse sick nursery cultivation condition to entire segregative line group
(identification in seedling stage and the dual disease resistance of Adult plant carries out artificial autocopulation's pollination at the disease-resistant single plant survived again and stays melon, fruit at
The ripe phase, each of harvesting is carved up from presentate carry out fruit type, external appearance characteristic, soluble sugar, taste quality, fruit section, list
Fruit weight and peel thickness and resistance to fortune property etc. (cover dark green sharp mouth item, skin depth < 1.2cm, center sugar > in the green bottom of high circle fruit, pericarp
12.5%, side sugar > 8.5%, meat is crisp, and fiber is few, the resistance to storage and transportation of fruit) comprehensive identification, screen acquisition from presentate melon kind
Son is to be isolated from the recombinant in the F3 generation of WF2201 to be.Autumn in the same year, by screening from presentate melon seed whole culturing and transplanting seedlings in greenhouse
In sick nursery, seedling stage and the dual disease resistance of Adult plant are carried out to each single plant of segregative line in the case where the autumn delays greenhouse sick nursery cultivation condition
Identification, selection seedling stage in florescence and Adult plant show disease-resistant plant progress artificial autocopulation's pollination and stay melon, fructescence pair
Respective presentate carves up the synthesis for not carrying out soluble sugar, taste quality, fruit section, single fruit weight and peel thickness and resistance to fortune property etc.
Identification, final election go out 13 F4 generation and are isolated from the recombinant preferred single melon.It is in the second year spring, the seed of 13 F4 generation single melon system of final election is whole
Seeding and seedling raising, when two leaf of seedling wholeheartedly when all transplant in greenhouse sick nursery, continue seedling under spring sowing greenhouse sick nursery cultivation condition
The identification of phase and the dual disease resistance of Adult plant, the single plant dual anti-to seedling stage and Adult plant carry out artificial autocopulation's pollination again and stay melon, obtain
213 mature single melons, the comprehensive identification through quality trait are obtained, final election goes out 9 F5 generation single melon systems.It is in autumn in the same year, in 9 F5 generations, are single
The seed difference culturing and transplanting seedlings of melon system continue autumn sowing greenhouse sick nursery cultivation condition lower seedling stage and Adult plant are double in greenhouse sick nursery
The identification of weight disease resistance, the disease-resistant single plant survived continue artificial autocopulation's pollination and stay melon, obtain 112 from presentate melon, then pass through
The comprehensive identification of quality trait, final election go out 5 F6 generation single melon systems.
After obtaining F6 generation single melon system, the present invention plants obtained F6 generation single melon system again in watermelon blight greenhouse disease
In garden, screening disease resistance and group's Agronomic, unanimously single melon system selfing is reserved seed for planting to get new diploid watermelon selfing is arrived
System.
After final election goes out F6 generation single melon system, the present invention is by obtained F6 generation single melon system seed whole culturing and transplanting seedlings in greenhouse sick nursery
In, then seedling stage and the dual disease resistance of Adult plant are carried out to each single plant (about 230 plants of seedlings of each list melon system) of each single melon system
Reinforcing identification, and Other Main Agronomic Characters (including plant type, blade profile, Growth period traits of each single all plant of melon system of survey
And fruit appearance character) consistent sex expression, screen disease resistance and the consistent single melon system of Agronomic of all plant, i.e.,
Obtain new diploid watermelon self-mating system.For the present invention after obtaining F6 generation single melon system, it is also preferable to include harvest F6 generation single melon
The single melon of the maturation of system carries out quality trait, and (skin depth < 1.2cm, center sugar > 12.5%, side sugar > 8.5%, meat is crisp, fiber
Comprehensive identification less), screening obtain F7 generation single melon, receipts are mixed after seed collecting.Autumn in third year separates from F7 generation single melon seed of mixed receipts
1500, culturing and transplanting seedlings observe the main agriculture of each plant seedling stage and Adult plant in the conventional greenhouse for being equipped with insect prevention separation net
Skill trait expression, florescence carry out artificial autocopulation's pollination respectively again and stay melon, and fructescence investigates the fruit type and appearance of each single melon
Feature, shows typical consistency, does not find any variation, determines monosystem performance specificity, consistency and stability, mixes
Each single melon seed of harvesting is closed, is named as ' V13-9-3 '.
The present invention also provides the diploid watermelon self-mating systems obtained using above-mentioned selection breeding in breeding watermelon quotient
Application in product new varieties.
The method of breeding watermelon commodity new varieties of the present invention, comprising: in the pollination distance being manually isolated, according to 1:
15 ratio plants diploid watermelon self-mating system plant of the present invention and other diploid watermelon self-mating system plant respectively；One
In the region of a isolation, the plant of 7~10 days in advance plantation diploid watermelon self-mating systems of the present invention, and retain diploid of the present invention
All lateral bines on watermelon plant；In another region being manually isolated, other diploid watermelon self-mating systems are planted
Plant all extracts lateral bines all on other diploid watermelon plant, only retains a main stem, and by described other two
All male flowers and male flower flower bud are all extractd on times body plant；Second female flower is given birth on other diploid watermelon plant,
Noon before that day will be opened, the female flower on other diploid watermelon plant is tightly covered, meanwhile, by west of the invention
Second day male flower that will be opened also all tightly covers on melon plant；When the next morning 8-11, with diploid of the present invention
The male flower opened is covered on watermelon plant to pollinate to the female flower opened is covered on other described diploid watermelon plant；Harvest
The seed of artificial pollination label fruit, obtains the kind of diploid watermelon cenospecies on other diploid watermelon plant
Son, as watermelon commodity kind.
The selection of diploid watermelon self-mating system provided by the invention and application are carried out below with reference to embodiment detailed
Explanation, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
According to the breeding for carrying out diploid watermelon self-mating system V13-9-3 the step of attached drawing 1:
Step 1: establish watermelon blight greenhouse Disease garden identification base: 1998, in District of Shanghai, selection topography was high and dry flat
Area is built in 750-1500m in smooth plot2The facility of scale links greenhouse (3-5 beam-connected greenhouse), and installs 1 in greenhouse surrounding
The antiseep film of meter Shen.The height for being inoculated with Races of F. oxysporum f. sp. niveum 1 in plastic shed soil using wheat inocalation method is caused a disease
Property bacterial strain, and cultivate within continuous 2 years susceptible variety of watermelon ' early good ', strengthen the pathogenic of withered germ of water-melon in plastic shed soil, build
At pathogenic strong and stable watermelon blight greenhouse Disease garden identification base.
Step 2: formulating disease-resistant good quality and high output watermelon recombination system: 1. spring (mid or late Febuary) in 2001, will
' Sugarlee ' culturing and transplanting seedlings select seedling stage and Adult plant to show disease-resistant single plant progress artificial autocopulation and award in greenhouse sick nursery
Powder stays fruit, and the maturity period harvests the normal fruit of growth and development and carries out species test identification, obtains center sugar and is greater than 11%, fruit section flesh
Color uniformly, single melon self-mating system Sugarlee-1 without hollow white muscle.2. autumn (late July) in 2001, by the disease-resistant of acquisition
Single melon seed seedling-raising plantation of high-quality self-mating system Sugarlee-1 selects seedling stage and Adult plant to show disease-resistant in greenhouse sick nursery
Single plant carries out artificial autocopulation's pollination and stays fruit, and the maturity period harvests the normal fruit of growth and development and carries out species test identification, obtains center sugar
Uniform, single melon self-mating system Sugarlee-2 without hollow white muscle greater than 11%, fruit section flesh color.3. (2 months spring in 2002
The middle ten days and the last ten days), by single melon seed seedling-raising plantation of the disease-resistant high-quality self-mating system Sugarlee-2 of acquisition in greenhouse sick nursery, select seedling stage
Disease-resistant single plant progress artificial autocopulation's pollination is showed with Adult plant and stays fruit, and the maturity period harvests the normal fruit of growth and development and carries out
Species test identification, acquisition center sugar uniform, self-mating system Sugarlee-3 without hollow white muscle greater than 11%, fruit section flesh color.④
Autumn (late July) in 2002 plants single melon seed seedling-raising of the disease-resistant high-quality self-mating system Sugarlee-3 of acquisition in big
Canopy sick nursery, by ' seed seedling-raising of early good (8424) ' variety of watermelon is planted in conventional greenhouse.Florescence, with seedling in Sugarlee-3
Phase and Adult plant show disease-resistant single plant as female parent, are that male parent carries out sexual hybridization with ' early good (8424) ', obtain high-quality anti-
The watermelon composite-crossing recombination system WF2201 of sick high yield.5. in mid or late October, 2002, by the composite-crossing recombination system of acquisition
The seed arrangement of WF2201 to the base Sanya, Hainan Nan Fan bypass the immediate leadership plus for plantation, florescence carries out stringent be manually isolated certainly
Fruit is stayed in friendship, the maturity period harvest WF2201 from presentate melon seed, obtaining F2 generation is isolated from the recombinant and is.
Step 3: the selfing systems of orientation step by step of high-quality disease-resistant high yield diploid watermelon new lines selection: i.e. using building up
Greenhouse wilt disease Disease garden identification base, seedling stage is carried out to the High quality and diseases resistance high yield watermelon recombination system of initiative and Adult plant is dual anti-
The continuously-directional selfing system of characteristic of disease selects.
In spring in 2003 (2 months-June), it is that single melon seed seedling-raising is planted that the F2 generation of the numerous acquisition in the south WF2201, which is isolated from the recombinant,
In greenhouse sick nursery, sow in spring under greenhouse sick nursery cultivation condition to entire segregative line group (early period low temperature, later period high temperature) seedling
The identification of phase and the dual disease resistance of Adult plant carry out artificial autocopulation's pollination at the disease-resistant single plant survived again and stay melon, fruit maturation
Phase, by each of harvesting from presentate carve up not carry out fruit shape, Characteristics of fruit peel, pulp soluble sugar, taste quality, fruit section,
Single fruit weight and peel thickness and resistance to fortune property etc. (cover dark green sharp mouth item, skin depth < 1.2cm, center sugar in the green bottom of fruit subcircular, pericarp
> 12.5%, side sugar > 8.5%, meat is crisp, fiber is few, and fruit section is without the white muscle in cavity, single fruit weight > 5kg, and Pi Ren is not easy
Dehiscent fruit etc.) comprehensive identification, preferably being selected from the F3 generation that presentate melon seed is WF2201 is isolated from the recombinant and is.(July 26 autumn in the same year
Day), in preferred F3 generation, is delayed into greenhouse sick nursery cultivation condition in the autumn in greenhouse sick nursery from presentate melon seed whole culturing and transplanting seedlings
Under the identification in seedling stage and the dual disease resistance of Adult plant is carried out (early period high temperature, later period low temperature) to each single plant of segregative line group, bloom
Selection seedling stage phase and Adult plant show disease-resistant plant progress artificial autocopulation's pollination and stay melon, and fructescence is to respective presentate melon
Fruit shape, Characteristics of fruit peel, pulp soluble sugar, taste quality, fruit section, single fruit weight and peel thickness and resistance to fortune are carried out respectively
Deng comprehensive identification, it is single melon that final election 13 F4 generation, which is isolated from the recombinant,.
The seed whole seeding and seedling raising of 13 F4 generation single melon system of final election is obtained 3316 by the spring in 2004 (2 months-June)
Surviving seedling, when two leaf of seedling wholeheartedly when all transplant in greenhouse sick nursery, continue seedling under spring sowing greenhouse sick nursery cultivation condition
The identification of phase and the dual disease resistance of Adult plant, the single plant dual anti-to seedling stage and Adult plant carry out artificial autocopulation's pollination again and stay melon, obtain
213 mature single melons, the comprehensive identification through quality trait are obtained, final election goes out 9 F5 generation single melon systems.
Autumn in the same year (August-November), by the seed difference culturing and transplanting seedlings of 9 F5 generation single melon systems in greenhouse sick nursery, continue into
The identification in row autumn sowing greenhouse sick nursery cultivation condition lower seedling stage and the dual disease resistance of Adult plant, the disease-resistant single plant survived continue people
Work pollination self stays melon, obtains 112 from presentate melon, the comprehensive identification through quality trait, final election goes out 5 F6 generation single melon systems.
Spring in 2005 (2 months-June), by 5 of final election single melon systems (WR25-1, WR25-2, WR25-3, WR25-4, WR25-
5) culturing and transplanting seedlings carry out each single plant (about 230 plants of seedlings of each list melon system) of each single melon system in greenhouse sick nursery respectively
The reinforcing identification of seedling stage and the dual disease resistance of Adult plant, and each Dan Guaxi group Other Main Agronomic Characters of survey (including each plant
Strain plant type, blade profile, Growth period traits and fruit appearance character) consistent sex expression, final election list melon system WR25-4.Harvest single melon
It is that the single melon of maturation of WR25-4 carries out quality trait (meat is crisp by center sugar > 12.5%, side sugar > 8.5%, fiber is few, fruit
For real section without the white muscle in cavity, skin depth < 1.2cm, pericarp are tough) comprehensive identification, preferably 21 F7 generation single melons mix to receive after seed collecting and (obtain
Obtain 4055 seeds).
Autumn in 2005, separate 1500 from the F7 of mixed receipts generation single melon seed, culturing and transplanting seedlings are in being equipped with insect prevention isolation
In the conventional greenhouse of net, observe the sex expression consistent with the Other Main Agronomic Characters of Adult plant of each plant seedling stage, florescence again respectively into
Pedestrian's work pollination self stays melon, and fructescence investigates the fruit type and external appearance characteristic of each single melon, show typical consistency and
Stability does not find any variation, determines monosystem performance specificity, consistency and stability, and mixing harvests each single melon seed,
It names as ' V13-9-3 '.
The main advantage characteristic of diploid watermelon self-mating system ' V13-9-3 ' of the present invention:
Seed is big, and growth of seedling is healthy and strong: every 75~85 grams of 1000 weights of its seed, be conventional diploid watermelon seed
1.5~2.5 times；
The comprehensive resistance of plant is strong, and growing way is strong, especially under low-light weather conditions overcast and rainy more than southern early spring low temperature, easily
Bear fruit, single plant is beared fruit 1 or more；
Fruit is big, resistance to storage and transportation: single melon weighs 6~9 kilograms, is 1.2~1.5 times of fruit type watermelon in routine.Skin depth 1.1~
1.3cm, pericarp is tough, resistance to storage and transportation.
Pulp high-quality flavor: the dense pink of pulp, 12.5%~13.5% or more center sugar, side sugar 8.5%~
9.5%, delicacy that meat is crisp, fiber is few, and taste flavor is excellent；
Seedling stage and the dual anti-Watermelon Fusarium Wilt biological strain 1 of Adult plant.
Has special DNA fingerprinting (NW2NX1NY2NZ2TW2TX1TY1TZ2SI1SF1SG2OT2OS1OB1WX1RW1
RT2GW2GS1)。
Embodiment 2
The production method of diploid watermelon self-mating system V13-9-3 seed:
Watermelon plant of the invention is planted in conventional greenhouse or open country according to conventional growth of watermelon technology；
In the plant blossom phase, it will open on watermelon plant of the present invention on the day before second female flower mentions and tightly cover, second
It is open to female flower, then carries out stringent artificial autocopulation's pollination, and be marked；
Fructescence harvests the ripening fruits that label artificial autocopulation pollination is tied on plant of the present invention；
The seed for digging out the tied ripening fruits of plant of the present invention is placed in clean fermenter, at room temperature fermentation process 8
~10 hours, then rinse with clear water the mucus on surface well, it is placed under sunlight and dries 2 days, then be placed in 35~40 DEG C of baking ovens
Drying and processing drops to 7%~8% to moisture, is sealed up for safekeeping, and storage saves.
Embodiment 3
The breeding of Shen honey 968:
Spring in 2008 plants the diploid watermelon self-mating system ' W6-9-1 ' with desired objective trait of incubation in normal
It advises in greenhouse, by diploid watermelon self-mating system ' V13-9-3 ' of the present invention plantation in greenhouse sick nursery, the plant blossom phase, acquisition is big
Male flower pollen open on diploid watermelon self-mating system ' V13-9-3 ' plant of the present invention in canopy sick nursery, to being planted in conventional greenhouse
Diploid watermelon self-mating system ' W6-9-1 ' plant on mention the female flower that covers of the previous day and carry out artificial pollination, and mark, fruit
The real maturity period acquires single melon seed of label, that is, obtains the seed of diploid hybrid watermelon Combination nova ' WF29-9 '.
2010, it is control with ' early good ', spring sowing greenhouse has been carried out to diploid hybrid watermelon Combination nova ' WF29-9 ' and has been set
The continuous crop qualification test under the different cultivation modes such as cultivation and covering with ground sheeting outdoor cropping is applied, ' WF29-9 ' performance is resistance to heavy
Stubble, easily bear fruit, single fruit is great, yielding ability is good, and fruit quality is extremely excellent, storage tolerance, and comprehensive disease resistance and economical character are significantly excellent
In control ' early good ', tentatively reach breeding project target.2011-2012, with ' early good ' for check variety, continuous 2 years upper
Haiti area watermelon continuous cropping base has carried out multiple spot area test product to ' WF29-9 ' and has compared qualification test.2013-2014, continuous 2 years
Watermelon main cultivation base in District of Shanghai has carried out continuous crop pilot production and demonstration to ' WF29-9 ', and ' WF29-9 ' is big in each pilot
Tian Junwu wilt disease occurs, and shows resistance to continuous cropping, and the plant for compareing ' early good ' in each pilot withers rate up to 90% or more.Together
When, ' WF29-9 ' shows easy cultivation management, easily bears fruit, and fruit is high round, blooms to fruit maturation about 35 days, pulp is dense pink
Color, 12.5% or more center sugar, 8.5% or more side sugar, meat is crisp succulence, fiber are few, and taste quality is splendid.Its skin depth is about
1.03 centimetres, pericarp is tough, storage tolerance, mean fruit weight 5.34kg, commodity melon yield 3304.3kg/667m2, disease resistance and product
Matter and yield traits are significantly better than control ' early good ', name as ' Shen honey 968 '.
' Shen honey 968 ' has obtained National Botanical new varieties power protection authorization (CNA20150464.8), which is planting on an equal basis
Under the conditions of training, compared with similar other diploid hybrid variety of watermelon, have the characteristics that as shown in table 1:
The variety of watermelon and similar variety protection that table 1. is bred as using the present invention
In conclusion being cultivated the present invention provides a kind of selection of diploid watermelon self-mating system and application
Parent of the diploid watermelon self-mating system particularly suitable as cultivating diploid hybrid new water melon breed, can be used as male parent or mother
This, and other diploid watermelon hybridizations between selfed lines, and the characteristic hereditary of its high-quality and high resistance to wilt good can be given to its hybridization one
Generation.And hybridization hybrid watermelon new varieties obtained have the quality and flavor of high standard compared with similar common variety of watermelon
And anti-watermelon blight and high yield characteristics, the resistance to storage and transportation of fruit are not easy the dehiscent fruit of falling flesh, therefore by the common of consumers and producers
Approve.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of selection of diploid watermelon self-mating system, comprising the following steps:
1) by anti-blight Watermelon Parent Seeding planting in watermelon blight greenhouse sick nursery, the disease-resistant single plant screened again into
Fruit is stayed in the continuous three generations's selfing of row, obtains self-mating system Sugarlee-3；
2) it is female parent with the self-mating system Sugarlee-3, carries out sexual hybridization using high-quality variety of watermelon of easily bearing fruit as male parent, obtain
To watermelon composite-crossing recombination system WF2201；
3) watermelon composite-crossing recombination system WF2201 progress artificial autocopulation is stayed into fruit, obtaining F2 generation is isolated from the recombinant and is；
4) being isolated from the recombinant the F2 generation is plantation in watermelon blight greenhouse sick nursery, screens disease-resistant single plant and carries out continuous four
Fruit is stayed for artificial autocopulation, obtains F6 generation single melon system；
5) the F6 generation single melon system is planted respectively in watermelon blight greenhouse sick nursery, screens disease resistance and Agronomic
Consistent list melon system is diploid watermelon self-mating system.
2. selection according to claim 1, which is characterized in that step 1), step 4) and step 5) the disease resistance packet
Include: seedling stage is disease-resistant and Adult plant is disease-resistant.
3. selection according to claim 1, which is characterized in that step 1) the anti-blight Watermelon Parent is
‘Sugarlee’。
4. selection according to claim 1, which is characterized in that step 2) the high-quality variety of watermelon of easily bearing fruit is ' early
It is good '.
5. selection according to claim 1, which is characterized in that the step 5) economical character includes: skin depth <
1.2cm, center sugar > 12.5%, side sugar > 8.5%, meat is crisp and fiber is few.
6. the diploid watermelon self-mating system that the selection breeding of any one of Claims 1 to 5 obtains is disease-resistant high-quality in breeding
Application in watermelon range of goods.
7. applying according to claim 6, which is characterized in that using the diploid watermelon self-mating system as male parent.
8. applying according to claim 7, which is characterized in that the planting number ratio of the male parent and female parent is 1:15.
9. according to the application of claim 7 or 8, which is characterized in that the male parent implantation time is earlier than 7~10d of female parent.
10. according to the application of claim 7 or 8, which is characterized in that extract all lateral bines of the female parent, only retain one
Main stem, and male flowers all in main stem and male flower flower bud are all extractd.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811406802.7A CN109287473B (en) | 2018-11-23 | 2018-11-23 | Breeding method of diploid watermelon inbred line and application of bred diploid watermelon inbred line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811406802.7A CN109287473B (en) | 2018-11-23 | 2018-11-23 | Breeding method of diploid watermelon inbred line and application of bred diploid watermelon inbred line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109287473A true CN109287473A (en) | 2019-02-01 |
| CN109287473B CN109287473B (en) | 2020-05-22 |
Family
ID=65143709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811406802.7A Active CN109287473B (en) | 2018-11-23 | 2018-11-23 | Breeding method of diploid watermelon inbred line and application of bred diploid watermelon inbred line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109287473B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150056A (en) * | 2019-07-01 | 2019-08-23 | 上海市农业科学院 | A kind of seed growing method keeping anti-blight variety of watermelon resistance |
| CN116548301A (en) * | 2023-05-19 | 2023-08-08 | 上海市农业科学院 | Breeding method and application of new variety (line) of compound disease-resistant watermelon |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094834A (en) * | 2013-04-01 | 2014-10-15 | 上海市农业科学院 | Breeding method for small-fruit type citrullus lanatus variety |
| EP3524049A1 (en) * | 2014-06-27 | 2019-08-14 | Seminis Vegetable Seeds, Inc. | Methods and assays for male sterile watermelon |
-
2018
- 2018-11-23 CN CN201811406802.7A patent/CN109287473B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104094834A (en) * | 2013-04-01 | 2014-10-15 | 上海市农业科学院 | Breeding method for small-fruit type citrullus lanatus variety |
| EP3524049A1 (en) * | 2014-06-27 | 2019-08-14 | Seminis Vegetable Seeds, Inc. | Methods and assays for male sterile watermelon |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150056A (en) * | 2019-07-01 | 2019-08-23 | 上海市农业科学院 | A kind of seed growing method keeping anti-blight variety of watermelon resistance |
| CN116548301A (en) * | 2023-05-19 | 2023-08-08 | 上海市农业科学院 | Breeding method and application of new variety (line) of compound disease-resistant watermelon |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109287473B (en) | 2020-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dingkuhn et al. | Growth and yield potential of Oryza sativa and O. glaberrima upland rice cultivars and their interspecific progenies | |
| Louette et al. | In situ conservation of maize in Mexico: genetic diversity and maize seed management in a traditional community | |
| CN104839010B (en) | A kind of selection of paddy rice High quality and diseases resistance is high temperature resistant two-line sterile line | |
| CN103975849B (en) | A kind of precocity, the breeding of disease-resistant, the pale reddish brown Broccoli of loose type and breeding method | |
| KR100735510B1 (en) | Tetraploid watermelons producing small fruits | |
| Amanze et al. | Selection of yam seeds from open pollination for adoption in yam (Dioscorea rotundata Poir) production zones in Nigeria | |
| Singh et al. | Basmati rice of India | |
| CN101444184A (en) | More efficient hot pepper breeding method | |
| CN109287473A (en) | A kind of application of the diploid watermelon self-mating system of the selection and breeding of diploid watermelon self-mating system | |
| CN105210850B (en) | A kind of method for creating for viewing and admiring tender flower stalk germ plasm resource | |
| CN104604671A (en) | Breeding method for novel vegetable soybean variety with wide adaptability | |
| CN111374045A (en) | Tartary buckwheat crossbreeding method | |
| CN109329043A (en) | A kind of selection of small seed type pumpkin rootstock kind | |
| Teferi et al. | Adaptation trail of different improved hot pepper (Capsicum species) varieties under Gedeo Zone, Dilla, Ethiopia | |
| CN105075854A (en) | Breeding method for early-maturing and disease-resistant broccoli | |
| CN108770678A (en) | A kind of crossbreeding selection of sea rice and Restoring Line of Japonica Rice | |
| KR102061227B1 (en) | Jeju Cactaceae species new variety of Jekukbaegnyuncho | |
| CN112715303A (en) | Breeding method for resistance of cassava tetranychus cinnabarinus | |
| CN101433179A (en) | Method for producing diploid seedless water melons | |
| CN107896980A (en) | A kind of selection of the Chinese cabbage germ plasm resource of suitable for mechanization harvesting | |
| CN110367117A (en) | It is a kind of cook after pulp be not easy the variety of luffa selection of brown stain | |
| KR102342057B1 (en) | Cold Stress and Clubroot Disease Resistant Novel Chinese Cabbage Variety | |
| CN107347629B (en) | A kind of method for creating for the tender flower stalk germ plasm resource being suitble to disposable harvest operation | |
| CN106305404A (en) | Breeding method of disease-resistant loose type broccoli | |
| Tamilselvi et al. | Development of interspecific hybrid rootstocks using Cucurbita moschata Duch ex. Poir and Cucurbita maxima lines. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |