CN110367117A - It is a kind of cook after pulp be not easy the variety of luffa selection of brown stain - Google Patents
It is a kind of cook after pulp be not easy the variety of luffa selection of brown stain Download PDFInfo
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- CN110367117A CN110367117A CN201910797193.0A CN201910797193A CN110367117A CN 110367117 A CN110367117 A CN 110367117A CN 201910797193 A CN201910797193 A CN 201910797193A CN 110367117 A CN110367117 A CN 110367117A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses the variety of luffa selections that pulp after a kind of cook is not easy brown stain, belong to field of plant variety breeding technology.Existing sponge gourd Luffa aegyptiaca varieties of plant is mostly used for stir-frying and eating or cooking, but universal problems faced is the generation brown stain easy to oxidize during the cooking process of luffa-smooth loofah pulp, affects appearance and reduces nutritive value.The present invention is by distant hybridization, mostly for backcrossing and selfed breeding, the excellent genes that pulp is not easy brown stain after Guangdong sponge gourd Luffa acutangula is cooked are imported into sponge gourd Luffa aegyptiaca, cultivate the sponge gourd new varieties XMR329 that pulp after a kind of cook is not easy brown stain.The new varieties appearance is similar to luffa-smooth loofah, fruit uniformity end to end, be about 20 centimetres, it is about 6 centimetres wide, single fruit weighs about 400 grams, and the grayish green wounded in the battle point of pericarp, pulp is not easy brown stain after cooking, best in quality.
Description
Technical field: the invention belongs to field of plant variety breeding technology, and in particular to pulp is not easy brown stain after one kind is cooked
Variety of luffa selection.
Background technique: there are two types of Curcurbitaceae (Cucurbitaceae) Luffa (Luffa Mill) plant is total in China, point
It Wei not sponge gourd Luffa aegyptiaca and Guangdong sponge gourd Luffa acutangula.Guangdong sponge gourd Luffa acutangula is again
Name angular sponge gourd, eight rib melons, in Guangdong, Guangxi, Fujian and Hainan province, cultivation is more, remaining province is then seldom cultivated.Guangdong
It is the crisp sweet tea of sponge gourd meat, best in quality, it is suitble to stir-fry and eat, and pulp hardly happens brown stain after cooking, but yielding ability, adaptability, anti-
Inverse property is not so good as sponge gourd;And sponge gourd Luffa aegyptiaca, also known as without rib sponge gourd, water melon, have in China major part province
Cultivation, wide adaptability and yielding ability is good, commercial variety is also more, the continuous suitable cooking of the soft pine of the pulp of sponge gourd, but existing market
On most variety of luffa cook after the easy brown stain of pulp, affect appearance and quality.
Summary of the invention: the present invention easily occurs brown for most variety of luffa pulp under high-temperature cooking currently on the market
The problem of change, provide it is a kind of solve the problems, such as this breeding method, and selected suitable China's major part province cultivation silk
Melon kind.
Technical scheme is as follows:
With variety of luffa ' short bamboo filament melon ' make it is maternal, take Guangdong variety of luffa ' double excellent Guangdong sponge gourds ' as paternal hybrid after,
By being returned with 2 generations of ' double excellent Guangdong sponge gourds ' backcrossing, with 4 generations of ' short bamboo filament melon ', resulting materials be selfed the breeding of 6 generations again and
At.It is specifically as follows:
1. being made with variety of luffa ' short bamboo filament melon ' more excellent currently on the market maternal, ' double excellent with Guangdong variety of luffa
Guangdong sponge gourd ' it is that male parent carries out artificial pollination, the two flowering asynchronism, i.e. distant hybridization are solved the problems, such as with the method that pollen refrigerates
After obtain interspecific F_1 fertility seed;
2. kind of plant F1 plant and ' double excellent Guangdong sponge gourds ' make female parent with interspecific F_1 fertility plant, with ' double excellent Guangdong sponge gourds '
Make male parent, equally solves the problems, such as flowering asynchronism with the method that pollen refrigerates, be returned, obtain BC1F1 seed, next season kind
BC1F1 plant and ' double excellent Guangdong sponge gourds ' are planted, is female parent with BC1F1 plant, continues to make male parent with ' double excellent Guangdong sponge gourds ', work the
Secondary backcrossing, and obtain BC2F1 seed;
It is male parent progress with ' short bamboo filament melon ' 3. kind of plant BC2F1 plant and ' short bamboo filament melon ' are female parent with BC2F1 plant
Backcrossing, obtains BC1F1 ' seed;Lower season plantation BC1F1 ' plant and ' short bamboo filament melon ', are female parent with BC1F1 ' plant, with ' short bamboo
Sponge gourd ' it is that male parent is returned, BC2F1 ' seed, then plantation BC2F1 ' plant of lower season and ' short bamboo filament melon ' are obtained, with BC2F1 '
Plant be female parent, with ' short bamboo filament melon ' be male parent be returned, obtain BC3F1 ' seed, with same method again with ' short bamboo filament melon '
Backcrossing, similarly obtains BC4F1 ' seed.
4. kind planting BC4F1 ' plant, individual plant selfing pollination is carried out, after fruit of reserving seed for planting and single plant is carried out and reserves seed for planting respectively, preferentially enter
Next season plantation, evaluates emphatically the BPH resistant rice variety ability and taste of fruit under the pulp high-temperature cooking of each plant of fruit, needs
Bright is, it should be noted that early generation is selected.BC4F1 ' plant was continuously selfed for 5 generations according to breeding objective, per generation chooses 3
Single plant enters the next generation, and mixing is carried out after character is stablized and is reserved seed for planting to get the sponge gourd of brown stain is not easy to pulp after a kind of cook
Kind XMR329.
The beneficial effects of the present invention are: the pulp of Guangdong sponge gourd is resisted by distant hybridization, mostly for backcrossing and selfed breeding
Brown stain channel genes cultivate the variety of luffa XMR329 that pulp after a kind of cook is not easy brown stain into sponge gourd, which is protecting
Under the premise of demonstrate,proving better quality, while the good yielding ability of luffa-smooth loofah, resistance and prematureness are still kept, Comprehensive Traits are more
It is excellent, there is biggish market popularization value.
Specific embodiment: below with reference to embodiment, the present invention is further illustrated, and however, it is not limited to this.
Embodiment 1: it is a kind of cook after pulp be not easy brown stain variety of luffa XMR329 breeding.
Specifically comprise the following steps:
1. being made with variety of luffa ' short bamboo filament melon ' more excellent currently on the market maternal, ' double excellent with Guangdong variety of luffa
Guangdong sponge gourd ' it is that male parent carries out artificial pollination, wherein ' short bamboo filament melon ' is to introduce from Hainan in January, 2011, melon is 18-20 lis long
Rice, about 6.5 centimetres wide, single fruit weighs about 400 grams, and no rib, melon face wrinkle be green, few wax powder, ' double excellent Guangdong sponge gourds ' be 2 months 2012 from
Guangdong Jiangmen is introduced, and melon is 40-45 centimetres long, about 6 centimetres wide, and single fruit weighs about 450 grams, and melon rib green, melon dyeing defect are green, because ' short
Bamboo sponge gourd ' flowering time be 5:30-6:30, and the flowering time of ' double excellent Guangdong sponge gourds ' be 17:30-18:30, the two florescence
It does not meet, is difficult to hybridize in its natural state, therefore, in order to smoothly complete hybridization, need noon before that day to maternal ' short bamboo
Sponge gourd ' female flower carry out flower bud phase bagging, and the male flower of male parent ' double excellent Guangdong sponge gourds ' is collected, simultaneously the male flower gathered
It is placed on after being sealed with transparent valve bag in 4 DEG C of refrigerator and carries out cryo-conservation.The routine operation that male flower is collected is in male flower
Flower bud phase artificial bagging, be collected to Post flowering.In order to reduce labour cost, the present invention is not necessarily to prior bagging, but 16:
00 or so directlys adopt the male flower of the big flower bud phase of male parent (before i.e. male parent is bloomed), more efficient and without any bad compared to routine operation
It influences.Wait until next day 5:30-6:30, it is maternal ' after short bamboo filament melon ' female flower is open, to extract bagging, and male with the male parent of cryo-conservation
Flower is authorized on the column cap of female flower, due to being fewer, use 2-3 for the pollen amount maternal column cap relatively of male parent male flower
Male parent male flower authorizes the better effect on a female chapiter of female parent.Maternal female flower corolla is pinioned with cord after completion artificial pollination
Other pollen are isolated.After the completion of aforesaid operations, i.e., interspecific F_1 fertility seed is obtained after distant hybridization;
2. kind of 36 plants of F1 plant of plant and 6 plants ' double excellent Guangdong sponge gourds ' make female parent with interspecific F_1 fertility plant, with ' double excellent wide
Eastern sponge gourd ' make male parent, flowering asynchronism equally is solved the problems, such as with the method that pollen refrigerates, and is returned, obtains BC1F1 seed,
Next season plants 36 plants of BC1F1 plant and 6 plants ' double excellent Guangdong sponge gourds ', is female parent with BC1F1 plant, continues with ' double excellent Guangdong
Sponge gourd ' make male parent, make secondary backcrossing, and obtain BC2F1 seed;
3. kind of 36 plants of BC2F1 plant of plant and 6 plants ' short bamboo filament melon ' are female parent with BC2F1 plant, with ' short bamboo filament melon ' for father
This is returned, and BC1F1 ' seed is obtained;Lower season will plant 36 plants of BC1F1 ' plant and 6 plants ' short bamboo filament melon ', with BC1F1 ' plant
For female parent, it is that male parent is returned with ' short bamboo filament melon ', obtains BC2F1 ' seed, then lower season plants 36 plants of BC2F1 ' plant and 6
Strain ' short bamboo filament melon ' is female parent with BC2F1 ' plant, is that male parent is returned with ' short bamboo filament melon ', obtains BC3F1 ' seed, use
Same method is returned with ' short bamboo filament melon ' again, similarly obtains BC4F1 ' seed.
4. kind of 72 plants of BC4F1 ' plant of plant, carry out individual plant selfing pollination, every plant is reserved seed for planting fruit 3, due to the plant in the stage
Flowering time variation amplitude is still larger, it is therefore desirable to be resident for a long time and observe situation of blooming, after fruit of reserving seed for planting and carry out single plant point
Do not reserve seed for planting, preferentially enter and plant in next season, evaluate emphatically BPH resistant rice variety ability under the pulp high-temperature cooking of each plant of fruit and
Taste of fruit, it should be noted that it is noted that early generation is selected.BC4F1 ' plant is continuously selfed according to breeding objective
In 5 generations, per generation, choose 3 single plants and enter the next generation, and mixing is carried out after character is stablized and is reserved seed for planting to get pulp after one kind is cooked has been arrived
It is not easy the variety of luffa XMR329 of brown stain.
The feature of XMR329: ripe in the kind, growth potential is strong, and branchiness is strong, and appearance is similar to luffa-smooth loofah, carpocephalum
Tail uniformity, be about 20 centimetres, it is about 6 centimetres wide, single fruit weighs about 400 grams, and fruit rid is weak, the grayish green wounded in the battle point of pericarp, after cooking
Pulp is not easy brown stain, best in quality, and resistance is relatively strong, adaptability is wider.
The Main Cultivation Technology of XMR329: 1. seeding and seedling raisings.Needed before sowing seed soaking 10 hours, vernalization waits in germinating box
50% seed carries out hole tray sowing after showing money or valuables one carries unintentionally.2. field planting.The seeding row spacing suggestion of field planting is 60 centimetres * 100 centimetres.3. built.It presses
According to locality cultivation habit, A-frame or flat frame.4. Plant adjustment.1 meter of side shoot below of main stem is wiped out in time, disease
Leaf, yellow leaf will also extract in time and take field out of.5. timely collecting.It is harvested in time when fruit meets listing criteria, avoids shadow
Ring subsequent bear fruit.6. pest and disease damages such as integrated control cucumber beetle, aphid, hang spring worm, downy mildew, powdery mildew, virosis.
Embodiment 2: the quality preservation experiment of fruit.
Each kind harvests commodity phase fruit 5 at random, and requires the maturity of each fruit, fruit size consistent,
And fruit is sliced, every piece of pulp is about 0.5 centimetre wide, and the pulp piece of each kind is divided into four parts.It is put into appropriate clear water, cuts
Pulp piece enters in pot, and pulp piece wraps with gauze and marked kind name, according to different time gradient and boil observation each
The fruit browning situation of kind.Experimental result is as follows:
Kind | XMR329 | Short bamboo filament melon | Double excellent Guangdong sponge gourds |
Fresh fruit texture (description) when adopting | It is relatively soft | It is soft | It is hard crisp |
Clear water boils 1 minute fruit browning degree | Not brown stain | Not brown stain | Not brown stain |
Clear water boils 3 minutes fruit browning degree | Not brown stain | Slightly brown stain | Not brown stain |
Clear water boils 5 minutes fruit browning degree | Not brown stain | Brown stain | Not brown stain |
Clear water boils 7 minutes fruit browning degree | Slightly brown stain | Brown stain | Not brown stain |
Above is only a specific embodiment of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Made any modification, equivalent replacement etc., should all be included in the protection scope of the present invention within principle.
Claims (4)
1. it is a kind of cook after pulp be not easy the variety of luffa selection of brown stain, which is characterized in that with variety of luffa ' short bamboo filament
Melon ' make it is maternal, take Guangdong variety of luffa ' double excellent Guangdong sponge gourds ' as paternal hybrid after, by 2 generations with ' double excellent Guangdong sponge gourds '
Backcrossing, the 4 generations backcrossing with ' short bamboo filament melon ', resulting materials are selfed the breeding of 6 generations again and are formed.
Specifically comprise the following steps:
(1) made with variety of luffa ' short bamboo filament melon ' maternal, artificial for male parent progress with Guangdong variety of luffa ' double excellent Guangdong sponge gourds '
Pollination, obtains interspecific F_1 fertility seed after distant hybridization;
(2) F1 plant is planted, female parent is made with interspecific F_1 fertility plant, male parent is made with ' double excellent Guangdong sponge gourds ', is returned, is obtained
BC1F1 seed, next season plant BC1F1 plant, are female parent with BC1F1 plant, continue to make male parent with ' double excellent Guangdong sponge gourds ', make
Secondary backcrossing, and obtain BC2F1 seed;
(3) BC2F1 plant is planted, is female parent with BC2F1 plant, then be returned with ' short bamboo filament melon ' for male parent, obtains
BC1F1 ' seed;Lower season will plant BC1F1 ' plant, be female parent with BC1F1 ' plant, be that male parent is returned with ' short bamboo filament melon ',
BC2F1 ' seed is obtained, then lower season plants BC2F1 ' plant, be female parent with BC2F1 ' plant, is male parent progress with ' short bamboo filament melon '
Backcrossing obtains BC3F1 ' seed, and same method is returned with ' short bamboo filament melon ' again, similarly obtains BC4F1 ' seed.
(4) BC4F1 ' plant is planted, carries out individual plant selfing pollination, and carry out single plant and reserve seed for planting respectively, preferentially enters and plants in next season,
The BPH resistant rice variety ability and taste of fruit under the pulp high-temperature cooking of each plant of fruit are evaluated emphatically, according to breeding objective handle
BC4F1 ' plant was continuously selfed for 5 generations, and character carries out mixing to reserve seed for planting after stablizing being not easy brown stain to get to pulp after a kind of cook
Variety of luffa XMR329.
2. it is according to claim 1 it is a kind of cook after pulp be not easy the variety of luffa selection of brown stain, it is characterised in that
It is authorized on the female chapiter of female parent in step (1) with 2-3 male parent male flower.
3. it is according to claim 1 it is a kind of cook after pulp be not easy the variety of luffa selection of brown stain, it is characterised in that
Maternal plant plants 36 plants in step (2) and (3), and paternal plant plants 6 plants.
4. it is according to claim 1 it is a kind of cook after pulp be not easy the variety of luffa selection of brown stain, it is characterised in that
BC4F1 ' plant plants 72 plants in step (4), and chooses 3 single plants after being selfed and enter the next generation.
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CN115812590A (en) * | 2022-12-06 | 2023-03-21 | 成都农业科技职业学院 | Crossbreeding and breeding method of low-browning towel gourds |
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CN115812590A (en) * | 2022-12-06 | 2023-03-21 | 成都农业科技职业学院 | Crossbreeding and breeding method of low-browning towel gourds |
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