CN105248271A - Environmental-impact-free method for creating upright and compact type germplasm resources of Chinese flowering cabbage - Google Patents
Environmental-impact-free method for creating upright and compact type germplasm resources of Chinese flowering cabbage Download PDFInfo
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Abstract
An environmental-impact-free method for creating upright and compact type germplasm resources of Chinese flowering cabbage is characterized in that a Suzhou green type common Chinese cabbage inbred line Q2, a Suzhou green type Chinese flowering cabbage inbred line G11 as well as Lubao 60 tian Chinese flowering cabbage G5 from conventional varieties of Guangdong Chinese flowering cabbage are taken as parent materials, and the method comprises the following steps: G5 as a female parent and Q2 as a male parent are hybridized, and first-filial generation seeds 1F1 are obtained; G5 as a recurrent parent and 1F1 as the male parent are backcrossed, and BC1F1 is obtained; BC1F2 is obtained through selfing of BC1F1; plants with the compact plant type in BC1F2 are selected for selfing, and BC1F3 is obtained; G11 as the female parent is hybridized with BC1F3, 2F1 is obtained, and BC1F4 is obtained through selfing of BC1F3; 2F1 as the female parent is hybridized with BC1F4, and 3F1 is obtained; plants with the compact plant type in 3F1 are selected for selfing, and 3F2 is obtained; plants with the compact plant type, upright blades and glossy dark green stalk color and free of lateral buds in 3F2 are selected for selfing, and 3F3 is obtained. The upright and compact type germplasm resources of the Chinese flowering cabbage are free of environmental impact during planting and integrate excellent commodity character genes such as no lateral buds, glossy dark green stalk color, no wax powder, shorter petioles and the like.
Description
Technical field
The present invention relates to vegetable breeding field, be specifically related to the method for creating of the upright compact germ plasm resource of a kind of tender flower stalk not affected by environment.
Background technology
Tender flower stalk belongs to the mutation of Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies tender flower stalk in Plant Taxonomy, another name cabbage heart, dish point, dish luxuriant, edible organs is a kind of sedge leaf of flower a kind of sedge and attachment, and being the Specialty vegetable originating in China, is one of vegetable species of common people's eating.In cultivated since long process, define some and be specifically designed to the landrace group producing tender flower stalk product, as the purple flowering stalk in Hubei, the cabbage heart in Guangdong, also have some other landrace in addition, as neat in Shanghai one cutter, Yangzhou tender flower stalk etc.
The blade of usual plant is subject to effective light irradiation, and more multi-biomass accumulation also can be more, therefore, mould the prerequisite that desirable plant type is high crop yield high-quality, for the tender flower stalk that planting density is larger, upright compact plant is the ideotype of high yield and high quality, this is because interfere with each other few between plant, can luminous energy be made full use of and ventilate, disease relatively can be less.Existing tender flower stalk upright compact germ plasm resource quantity is few and proterties is very easily affected by environment and change, the method for the upright compact germ plasm resource also not having a kind of initiative not easily affected by environment at present.Although some tender flower stalk kind declares that plant type is uprightly compact, find that the blade between the current most of plant in tender flower stalk kind field of applying blocked mutually in the middle and later periods according to our actual observation, improper ventilation is easily susceptible, and general commodity rate is lower.Upright Compactness is shown during only a few kind benign climate, but when environmental change especially at high temperature season, plant type just becomes loose, and commodity significantly declines, seriously constrain the further raising of flower stalk yield and quality, the plant type of existing kind is necessary to be improved.Moreover, sprouting of lateral bud proterties is one of important goods proterties of tender flower stalk, production require when tender flower stalk is gathered or begin to spend, lateral bud will not sprouted as far as possible or sprout not obvious, and existing germ plasm resource comprises cultivar sprouting of lateral bud proterties and is subject to the impact of the factor such as environment, sprouting of lateral bud the Characters is unstable, and the tender flower stalk needing initiative not easily affected by environment is without lateral bud germ plasm resource.
Current tender flower stalk breeding is main or based on the traditional approach such as line breeding, conventional cross-breeding, through seed selection for many years, the most resistance level of these conventional varieties is not high enough, and water and fertilizer utilization is low, and the space that yield and quality improves further is very little.Numerous research shows that tender flower stalk also exists obvious hybrid vigour, and can improve resistance by extremely significant increase yield, employing heterosis breeding mode is the inevitable choice of tender flower stalk breeding work from now on.In the practice process of heterosis breeding, find that the most of hybrid combination plant type of tender flower stalk becomes and more carry out, sprouting of lateral bud is many, and petiole is obviously elongated, and commodity significantly declines, and especially can increase the weight of performance under poor environment, production is difficult to application.In order to solve this key technical problem, the current measure mainly taked is that selection Parent petiole is short, plant type is uprightly compact, what require in strict conformity with this due to current tender flower stalk kind matter still lacks very much, effect is also not fully up to expectations generally, be necessary the upright compact germ plasm resource of tender flower stalk that a large amount of initiative is not affected by environment, to adapt to the demand of tender flower stalk heterosis breeding.
Suzhou common people have and eat the custom of SUZHOUQING(sic) type common white cauliflower a kind of sedge in early spring, are commonly called as dish point, due to common Chinese cabbage needs the vernalization of low temperature long-day could bolting, generally starting listing by the end of February, the supply phase is short, is only 1 month.In order to also have tender flower stalk to go on the market in other seasons, introduce a fine variety the tender flower stalk originating in south China (i.e. Guangdong cabbage heart) in recent years, because the dish point of mouthfeel and this real estate has obvious difference, market acceptance is not high, development is comparatively slow, existing tender flower stalk kind is difficult to solve this technical problem, the seed selection kind that is applicable to Suzhou consumption habit must solve above-mentioned technical problem, be necessary the germ plasm resource of formulating applicable Suzhou consumption mouthfeel, to meet breeding demand.
In sum, in order to significantly improve flower stalk yield quality, need a large amount of tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud as breeding basis, also there is no the open report of correlation technique at present, secondly also need to formulate the germ plasm resource of applicable local consumption demand to expand tender flower stalk consumption area, how they are condensed together at present also without the open report of correlation technique, be necessary independently to invent the method for creating of a kind of tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud.
Summary of the invention
The invention provides the method for creating of the upright compact germ plasm resource of a kind of tender flower stalk not affected by environment.Utilize Guangdong cabbage heart conventional variety Lubao 60 tian (code name is G5), SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 and SUZHOUQING(sic) type tender flower stalk inbred line G11 as parent material, adopt traditional breeding method and multiple progeny selection methods such as hybridizing and backcross, when plant type is compact, upright blade, beginning flower, lateral bud is not sprouted and the proterties such as applicable Suzhou consumption mouthfeel is collected in same germplasm materials, formulate tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud, for the new varieties successfully selecting high yield and high quality have established Germplasm Bases.
For achieving the above object, the technical solution used in the present invention is: the method for creating of the upright compact germ plasm resource of a kind of tender flower stalk not affected by environment, select Guangdong cabbage heart conventional variety Lubao 60 tian, SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 and SUZHOUQING(sic) type tender flower stalk inbred line G11 as parent material, at least comprise the following steps:
The first step, with described Guangdong cabbage heart conventional variety Lubao 60 tian for female parent and described SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 are for paternal hybrid, obtains first-filial generation seed 1F1;
Second step, backcrosses for male parent for recurrent parent and described first-filial generation 1F1 with described Lubao 60 tian, obtains the first-filial generation seed BC1F1 of first backcross generation;
3rd step, obtains the second filial generation seed BC1F2 of first backcross generation with the first-filial generation BC1F1 selfing of described first backcross generation;
4th step, the plant selfing selecting plant type compact in the second filial generation BC1F2 of described first backcross generation obtains the hybridization three generations seed BC1F3 of first backcross generation;
5th step, with described SUZHOUQING(sic) type tender flower stalk inbred line G11 for maternal to hybridize with described hybridize three generations BC1F3 obtains first-filial generation seed 2F1, the hybridization four generation seed BC1F4 of the hybridization three generations BC1F3 selfing acquisition first backcross generation of described first backcross generation;
6th step, with described first-filial generation 2F1 for maternal to hybridize with the hybridization four generation BC1F4 of described first backcross generation obtains new first-filial generation seed 3F1;
7th step, the plant selfing selecting plant type compact in described first-filial generation 3F1 obtains second filial generation seed 3F2;
8th step, in described second filial generation 3F2, select that plant type is compact, upright blade, beginning flower time lateral bud not sprout and the plant selfing of a kind of sedge look glossy dark green obtains hybridization three generations seed 3F3, this hybridization three generations seed 3F3 is the upright compact germ plasm resource of tender flower stalk not affected by environment;
The morphological feature of described tender flower stalk upright compact germ plasm resource is that plant type is compact, upright blade, and when beginning to spend, lateral bud is not sprouted, and plant height is 36 ~ 38 centimetres, strain width is 19 ~ 21 centimetres, a kind of sedge look glossy dark green wax powder-free, and main a kind of sedge is high is 23 ~ 25 centimetres, main a kind of sedge is slightly 1.5 ~ 2.5 centimetres, and tender flower stalk weighs 59 ~ 62 grams; Blade is long 24.5 ~ 26 centimetres, and blade is wide 14 ~ 16 centimetres, long 5.5 ~ 7 centimetres of petiole, leaf green is glossy subcircular, a kind of sedge leaf petiole and a kind of sedge angle little, blade face is open and flat little with a kind of sedge angle.
Related content in technique scheme is explained as follows:
1, in such scheme, the morphological feature of described Guangdong cabbage heart conventional variety Lubao 60 tian is plant height 26 ~ 28 centimetres, strain 28 ~ 34 centimetres, base leaf blade oval, bottle green, long 17 ~ 19 centimetres of blade, blade is wide 7.5 ~ 9 centimetres, long 5 ~ 6.5 centimetres of petiole, wide 0.9 ~ 1.5 centimetre; A kind of sedge leaf is narrow avette, and tender flower stalk consolidation bar is even, and dark green glossy, main a kind of sedge is high 22 ~ 26 centimetres, and main a kind of sedge transverse diameter 1.5 ~ 1.8 centimetres, tender flower stalk weighs 35 ~ 40 grams.The Guangdong cabbage heart conventional variety got is introduced a fine variety in the source of described Lubao 60 tian, by the seed selection of Guangzhou vegetables Science Institute.
2, in such scheme, the morphological feature of described SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 is plant height 17 ~ 18 centimetres, strain 24 ~ 25 centimetres, Weight per plant 278 ~ 283 grams, the number of blade is 20 ~ 22, and property with a tight waist is good, blade subcircular, petiole cochlear, dark green leaf, the green white of petiole; During bolting, a kind of sedge green has draped over one's shoulders wax powder, and tender flower stalk plant type compact half is upright, petiole and interval short, a kind of sedge leaf petiole and a kind of sedge angle medium, blade face is open and flat medium with a kind of sedge angle.The source of described SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 is the excellent common Chinese cabbage individual plant that my unit selects from Suzhou farmers' vegetable plot, goes through the seed selection of multigeneration system, greatly improves than former colony regularity.
3, in such scheme, the morphological feature of described SUZHOUQING(sic) type tender flower stalk inbred line G11 is that plant type is compact, plant height 32 ~ 34 centimetres, strain 23 ~ 25 centimetres; There is wax powder on the green surface of a kind of sedge, and tender flower stalk weighs 49 ~ 51 grams, thick 1.5 ~ 2.2 centimetres of a kind of sedge, and a kind of sedge is high 21 ~ 22 centimetres; Leaf look green, blade subcircular, and blade is long 15 ~ 16 centimetres, and blade is wide 9 ~ 11 centimetres, long 5 ~ 6 centimetres of petiole, and petiole is wide 1.5 ~ 2.0 centimetres, a kind of sedge leaf petiole and a kind of sedge angle little, blade face is open and flat large with a kind of sedge angle.The source of described SUZHOUQING(sic) type tender flower stalk inbred line G11 is the plant of the easy bolting that my unit finds from common Chinese cabbage vegetable plot, Suzhou, by inbreeding of more generation optimum selecting, the common Chinese cabbage type tender flower stalk inbred line that the major traits obtained is stable, can be used for local tender flower stalk production in winter.
4, in such scheme, described hybridization three generations seed 3F3 is the upright compact germ plasm resource of tender flower stalk not affected by environment, the plant type gathered is now compact, upright blade, beginning flower time lateral bud not sprout and proterties such as applicable Suzhou consumption mouthfeel etc. is stable can entail lower generation, but now gene does not isozygoty, other important Agronomics are also inconsistent, preferentially can purify further as required, also can be used as the donor parents of merit gene in selection cross.
5, in such scheme, plant matter and refer to decision biological heredity proterties, and its hereditary information is passed to the genetic material of offspring from parental generation, be the entity of objective reality, genetics is called gene.Heredity, breeding and production there is all living things material of value all can be referred to as germ plasm resource, be cultivate the material base of new varieties from germ plasm resource the angle of breeding, the form of expression can be: landrace, improve the breed, the kind of new seed selection, introduced variety, seed, mutant, wild species, kindred plant, the various biotypes of artificial creation, vegetative propagation organ, individual cells, individual chromosome, individual gene, even DNA fragmentation etc.
6, in such scheme, backcrossing refers to two parents and hybridizes and hybridize with one of parent.Parent for repeatedly backcrossing is called recurrent parent, and recurrent parent is the object accepting improvement, is the recipient of beneficial traits (objective trait), also known as receptor parent, only have the parent of application during first time hybridization, it is the supplier of objective trait, therefore claims donor parents.
7, in such scheme, described tender flower stalk belongs to the mutation of Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies tender flower stalk on botany classification, mainly comprises the landrace that the purple flowering stalk in the areas such as the cabbage heart (Guangdong cabbage heart) that originates from South China and Hubei Province, Hunan, Chinese cabbage a kind of sedge and other areas are main purpose with tender flower stalk of gathering.Tender flower stalk can be subdivided into green tender flower stalk, purple flowering stalk and Chinese cabbage a kind of sedge according to the color on a kind of sedge surface, and tender flower stalk of the present invention is green tender flower stalk.Tender flower stalk can be divided into main a kind of sedge and side a kind of sedge according to a kind of sedge inserted part, and main a kind of sedge is that terminal bud germination is formed, and side a kind of sedge is that sprouting of lateral bud is formed, and tender flower stalk of the present invention is the type of edible main a kind of sedge.
8, in such scheme, described common Chinese cabbage belongs to the mutation of Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies common Chinese cabbage on botany classification, and common Chinese cabbage described in the present invention specifically refers to SUZHOUQING(sic) type.
Due to the utilization of technique scheme, design of the present invention and beneficial effect are: for current tender flower stalk without lateral bud upright compact germ plasm resource easily affected by environment, heterosis breeding need upright compact germ plasm resource not affected by environment and lack the technical problem such as tender flower stalk germ plasm resource of applicable Suzhou consumption mouthfeel, this invention contemplates the upright compact germ plasm resource of a kind of tender flower stalk not affected by environment, required merit is gathered in same germplasm materials.Different parent materials has respective pluses and minuses, wherein, mainly compact with a tight waist, a kind of sedge leaf petiole of plant type is with the angle of scape is less, petiole is short, leaf subcircular and mouthfeel are applicable to Suzhou District etc. to consider SUZHOUQING(sic) type common Chinese cabbage advantage, and shortcoming mainly needs that the vernalization of low temperature long-day could bolting be bloomed, breeding time is grown, often there is wax powder on a kind of sedge and blade face and pitch is shorter etc.; SUZHOUQING(sic) type tender flower stalk advantage is similar to SUZHOUQING(sic) type common Chinese cabbage, easier bolting, and shortcoming is that a kind of sedge and blade face often have wax powder, and to compare vegetative period obviously partially long with the slow cabbage heart originating from Guangdong; The cabbage heart advantage originating from South China be bolting without the need to the low temperature long-day, a kind of sedge look glossy dark green wax powder-free, joint is long moderate, and shortcoming is that upright compact plant kind matter is few, lateral bud more or less obviously sprouts and these proterties are normal easily affected by environment.Therefore, we utilize SUZHOUQING(sic) type common Chinese cabbage, SUZHOUQING(sic) type tender flower stalk and Guangdong cabbage heart as parent, adopt traditional breeding method and multiple progeny selection methods such as hybridizing and backcross, by plant type uprightly compact, begin flower time lateral bud not sprout and proterties such as applicable Suzhou consumption mouthfeel etc. is collected in same germplasm materials, formulate tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud.
Pass through our repeatedly field planting, high temperature season and favorable season are made comparisons, obviously the tender flower stalk germ plasm resource of visible the present invention's initiative still keeps lateral bud not sprout and upright compact plant, petiole and joint length are not all obviously elongated, and the upright compact germ plasm resource of traditional tender flower stalk at high temperature seems that plant type is carried out (petiole and scape angle obviously become large), petiole and internode obviously elongate, illustrate that the tender flower stalk that the present invention formulates is not affected by environment without lateral bud upright compact germ plasm resource, many merit genes such as best possible merchandise and applicable Suzhou consumption mouthfeel such as simultaneously also gathered a kind of sedge look glossy dark green wax powder-free and petiole is shorter.
Characteristic of the present invention is that people is for breaking geographical estrangement, the common Chinese cabbage of different ecological area and tender flower stalk gene is made to be able to mutual fusion, formulate the upright compact germ plasm resource of a kind of tender flower stalk not affected by environment, enrich tender flower stalk germplasm resource bank, start the new route of a tender flower stalk germplasm innovation.
Tender flower stalk belongs to the mutation of Cruciferae Brassica genus Vegetable Crops of Brassica Chinese cabbage subspecies tender flower stalk in Plant Taxonomy, special production is to spend the vegetables that a kind of sedge and a kind of sedge leaf are edible tissue, wherein cabbage heart type only the annual sowing area of Guangdong one province be no less than 200,000 hectares, the maximum vegetables of this province's sowing area, year needs about 1500 tons, seed, because great majority are conventional seeds, the output value about 3,000 ten thousand yuan.Tender flower stalk of the present invention upright compact germ plasm resource has gathered that plant type is compact simultaneously, upright blade, beginning flower time lateral bud do not sprout, the merit gene such as the shorter and applicable Suzhou consumption mouthfeel of a kind of sedge look glossy dark green wax powder-free, petiole, and these proterties when Various Seasonal is planted variable rate technology stablize, can genetic stability not affected by environment.If the high-quality compact germ plasm resource utilizing the present invention to formulate, then in conjunction with heterosis breeding technology, seed selection combination, then the market share may surpass hundred million yuan.Along with common people from had enough in the past meal to eat good things, eat rare and eat healthy etc. vary the diet direction change and population mobility strengthen, many local characteristic vegetables are cultivated with being no longer only confined to main product, introduced a fine variety to both at home and abroad, wherein tender flower stalk development is especially fast, consumption market constantly expands, both at home and abroad can be increasing for tender flower stalk high quality seed demand, therefore, application prospect of the present invention also can be become better and better.
Accompanying drawing explanation
Accompanying drawing 1 is the initiative flow chart of tender flower stalk without the upright compact germ plasm resource of lateral bud of the embodiment of the present invention;
Accompanying drawing 2 is that the tender flower stalk of the embodiment of the present invention shines without lateral bud upright compact germ plasm resource field individual plant;
Accompanying drawing 3 is that the tender flower stalk of the embodiment of the present invention shines one without lateral bud upright compact germ plasm resource field entirety;
Accompanying drawing 4 is that the tender flower stalk of the embodiment of the present invention shines two without lateral bud upright compact germ plasm resource field entirety.
Embodiment
The initiative target formulating novel germ plasm resource is needed according to current tender flower stalk breeding.
In the following description, involved parent material SUZHOUQING(sic) type common Chinese cabbage Q2 and SUZHOUQING(sic) type tender flower stalk G11 is the breeding material that our unit has by oneself, and parent material Lubao 60 tian draws the cabbage heart conventional variety from Guangzhou.
Agricultural Science Inst. of Taihu District of Jiangsu Prov. (Academy of Agricultural Sciences of Suzhou City) started to carry out the tender flower stalk not affected by environment initiative work without the upright compact germ plasm resource of lateral bud winter in 2007, the blank of this type of kind of matter method for creating has been filled up by years of researches, see the flow chart shown in accompanying drawing 1, concrete implementation step is as follows:
In December, 2007 in booth live SUZHOUQING(sic) type common Chinese cabbage inbred line Q2, plot area 3 square metres, final singling 60 strain; In February, 2008 in booth live Guangdong cabbage heart conventional variety Lubao 60 tian (code name G5), plot area 3 square metres, final singling 100 strain; The individual plant that florescence in April, 2008 selects 5 strain robust growth petioles short in G5 removes stamen as female parent, and in Q2, select the individual plant of 5 strain robust growth as male parent pollination hybridization, seed maturity mixing is gathered and obtained first-filial generation seed 1F1.
In August, 2008 in the live first-filial generation seed of booth 1F1 and G5, plot area 3 square metres, final singling 100 strain; The individual plant that florescence in October selects 5 strain robust growth petioles short in G5 removes stamen as female parent, in first-filial generation F1, select the individual plant of the short robust growth of 5 strain petiole as male parent pollination hybridization, seed maturity mixing is gathered and is obtained the first-filial generation seed BC1F1 of first backcross generation.
In February, 2009 is in the first-filial generation seed BC1F1 of the live first backcross generation of booth, plot area 3 square metres, final singling 100 strain, in the individual plant that the neat mouth phase selects that 10 strain petioles are short, leaf a kind of sedge look glossy dark green wherein, blade subcircular is similar with growing way healthy and strong breeding time, all the other plant are pulled out, bagging selfing, picking time gathers respectively, and every strain is got about 100 seeds and mixed the second filial generation seed BC1F2 obtaining first backcross generation.
In August, 2009 is in the second filial generation seed BC1F2 of the live first backcross generation of booth, plot area 9 square metres, final singling about 300 strain, find that there is in the neat mouth phase that more than 10 strain petioles are short especially, leaf a kind of sedge look glossy dark green and the rotund individual plant of blade, overall growing way is weaker than other plant, pull out all the other individual plants, above-mentioned plant is put fly net, October full-bloom stage every 2 days individual plants respectively indirect labor pollinate 2 times, seed maturity is gathered respectively, and every strain is got about 50 seeds and mixed the hybridization three generations seed BC1F3 obtaining first backcross generation.
In December, 2009 is in booth live SUZHOUQING(sic) type tender flower stalk inbred line G11, February next year is in the hybridization three generations seed BC1F3 of the live first backcross generation of booth, plot area is 3 square metres, final singling 100 strain, in G11, select 5 strain plant types compact in the neat mouth phase, petiole handle angle is minimum and individual plant that is growing way stalwartness is stand-by, all the other pull out, from BC1F3, select 10 strain plant types compact especially in the neat mouth phase, petiole is short especially, leaf a kind of sedge look glossy dark green, the individual plant of blade subcircular and growing way stalwartness is stand-by, all the other are pulled out, the individual plant chosen with G11 in the florescence in April is for individual plant that is maternal and that choose with BC1F3 is for the hybridization of male parent producing Crossbred, seed maturity is gathered respectively, each hybridization is got about 100 seed Homogeneous phase mixing Unified numbers and is obtained first-filial generation seed 2F1.The 10 strain BC1F3 chosen overlap with fly net by the same period before entering the early flowering season, October full-bloom stage every 2 days individual plants respectively indirect labor pollinate 2 times, seed maturity is gathered respectively, and every strain is got about 50 seeds and mixed the hybridization four generation seed BC1F4 obtaining first backcross generation.
In August, 2010 is in the hybridization four generation seed BC1F4 of booth live first-filial generation seed 2F1 and first backcross generation, plot area 3 square metres, final singling 100 strain, the neat mouth phase selects 10 strain plant types compact in 2F1, petiole short handle angle is little and individual plant that is growing way stalwartness is stand-by, all the other pull out, from BC1F4, select 10 strain plant types compact especially in the neat mouth phase, petiole is short especially, leaf a kind of sedge look glossy dark green, the individual plant of blade subcircular and growing way stalwartness is stand-by, all the other are pulled out, the individual plant chosen with 2F1 in the florescence in April is for individual plant that is maternal and that choose with BC1F4 is for the hybridization of male parent producing Crossbred, seed maturity is gathered respectively, each hybridization is got about 50 seed Homogeneous phase mixing Unified numbers and is obtained first-filial generation seed 3F1.
In February, 2011 is in booth live first-filial generation seed 3F1, plot area 9 square metres, final singling 300 strain, the neat mouth phase therefrom selects that plant type is compact, petiole is less compared with short handle angle, leaf nearly circle, leaf a kind of sedge look glossy dark green and normal and individual plant 20 strain of stalwartness of growth. and all the other are pulled out, early flowering season puts fly net, October full-bloom stage every 2 days individual plants respectively indirect labor pollinate 2 times, numbering respectively of gathering respectively after seed maturity obtains second filial generation seed 3F2.
In August, 2011 is in booth live second filial generation seed 3F2, each individual plant offspring Bo Yige community alone, plot area 3 square metres, final singling 100 strain, neat mouth phase find wherein 1 community plant show as plant type compact especially, begin flower time lateral bud is not sprouted, petiole is short-and-medium, petiole handle angle is minimum, upright blade, leaf subcircular, leaf a kind of sedge look glossy dark green, a kind of sedge is sturdy and grow normal healthy and strong plant, therefrom preferably 20 strain individual plants are reserved seed for planting in florescence cover net, the therefrom preferred good individual plant of 10 strain knot, numbering respectively of gathering respectively obtains hybridization three generations seed 3F3.
In February, 2012 is in booth live hybridization three generations seed 3F3, each individual plant offspring Bo Yige community alone, plot area 3 square metres, final singling 100 strain, it is not obvious that the neat mouth phase observes each community outward appearance difference, again according to plant totally strong condition and edible quality, emphasis is selected and remain 5 communities, each community the most excellent individual plant of 10 strains of selecting and remain again is listed, put fly net respectively, again according to the general performance of knot etc. picking time aspect, 2 communities of preferentially selecting and remain, each community focal selection 2 single-strain seeds numbering respectively of gathering respectively obtains hybridization four generation seed 3F4.
In August, 2012 is in booth live hybridization four generation seed 3F4; 4 individual plant offspring Bo Yige communities alone; plot area 3 square metres; final singling 100 strain, it is not obvious that neat mouth phase observes community difference, selects and remain the most excellent individual plant of 10 strains in each community; all the other are pulled out; put fly net respectively, each community shows excellent individual plant gather respectively in seed collection phase 2 strain knot of selecting and remain, and each individual plant is got about 100, seed respectively and mixed and obtain hybridization five generation seed 3F5.In addition, carry out field planting test assessment germplasm character to be measured and stability, hybridization four generation seed 3F4 and the upright compact germ plasm resource of traditional tender flower stalk is sowed in contrast respectively in July and October, utilize 128 point disk seedling growings, transplant during seedling age 4 leaf, cultivated area 10 square metres, seeding row spacing is 20 cm x 25 centimetres, plant 200 strains, in time entering picking time, field individual plant regularity is general, carry out observation to these individual plants to taste and front and back contrast, result shows that upright compact plant proterties is very stable, lateral bud is not also sprouted, mouthfeel is also fine, required merit all gathers in same germplasm materials, tentatively formulate tender flower stalk not affected by environment without the upright compact germ plasm resource of lateral bud, now gene homozygosity is general, can further selfing isozygoty as required.
In February, 2013 is in booth live hybridization five generation seed 3F5; plot area 3 square metres; final singling 100 strain; the neat mouth phase selects and remain the most excellent individual plant of 20 strains; all the other are pulled out; put fly net respectively, each community shows excellent individual plant gather respectively in seed collection phase 10 strain knot of selecting and remain, and each individual plant is got about 50, seed respectively and mixed and obtain hybridization six generation seed 3F6.
In August, 2013 is in booth live hybridization six generation seed 3F6; plot area 3 square metres; final singling 100 strain; the neat mouth phase selects and remain the most excellent individual plant of 20 strains; all the other are pulled out; put fly net respectively, each community shows excellent individual plant gather respectively in seed collection phase 10 strain knot of selecting and remain, and each individual plant is got about 50, seed respectively and mixed and obtain hybridization seven generation seed 3F7.In addition, carry out field planting test assessment germplasm character to be measured and stability, hybridization six generation seed 3F6 is sowed respectively in July and October, utilize 128 point disk seedling growings, transplant during seedling age 4 leaf, cultivated area 10 square metres, seeding row spacing is 20 cm x 25 centimetres, plant 200 strains, in time entering picking time, field individual plant regularity is better, carry out observation to these individual plants to taste and front and back contrast, result shows that upright compact plant proterties is very stable, lateral bud is not also sprouted, mouthfeel is also fine, required merit all gathers in same germplasm materials, be successful on the whole and formulated the upright compact germ plasm resource of tender flower stalk not affected by environment, now gene homozygosity is better, can further selfing isozygoty as required.
In February, 2014 is in booth live hybridization seven generation seed 3F7; plot area 3 square metres; final singling 100 strain; the neat mouth phase selects and remain the most excellent individual plant of 20 strains; all the other are pulled out; put fly net respectively, each community shows excellent individual plant gather respectively in seed collection phase 10 strain knot of selecting and remain, and each individual plant is got about 50, seed respectively and mixed and obtain hybridization eight generation seed 3F8.Now seed purity is very high, has gathered more merit without lateral bud upright compact kind matter.
In August, 2014 is in booth live hybridization eight generation seed 3F8; plot area 3 square metres; final singling 100 strain; the neat mouth phase selects and remain the most excellent individual plant of 20 strains; all the other are pulled out; put fly net respectively, each community shows excellent individual plant gather respectively in seed collection phase 10 strain knot of selecting and remain, and each individual plant is got about 50, seed respectively and mixed and obtain hybridization nine generation seed 3F9.In addition, carry out field planting test assessment germplasm character to be measured and stability, sow hybridization eight generation seed 3F8 and the upright compact germ plasm resource (contrast) of traditional tender flower stalk in July and October respectively, utilize 128 point disk seedling growings, transplant during seedling age 4 leaf, cultivated area 10 square metres, seeding row spacing is 20 cm x 25 centimetres, plants 200 strains, when entering picking time, to be measured kind of matter field individual plant regularity is fine, selects the representative individual plant of 20 strain to carry out examining seedling respectively.In table 1, in conjunction with the angle data variation of the plant height of front and back date of seeding, strain width and maximum leaf petiole and scape, visible to be measured kind of matter is compared very little, to be measured kind of the matter of conventional upright compact germ plasm resource upright Compactness change degree of convergence lateral bud when front and back date of seeding tender flower stalk begins flower and is not all sprouted, and contrast lateral bud germination rate change degree of convergence up to 485.2%, visible to be measured kind of matter is highly stable not affected by environment, and petiole long change degree of convergence is also very little, illustrates that exterior quality is very stable.Mouthfeel well applicable Suzhou consumption demand is tasted through sampling many people, required merit all gathers in same germplasm materials, in conjunction with above 2 years field test test, successfully to formulate tender flower stalk not affected by environment and uprightly gather type germ plasm resource without lateral bud, and gene homozygosity is very high, see accompanying drawing 2 ~ 4.
Table 12014 to be measured kind of matter Some Agronomic Traits summary sheet year summer and autumn
Above-described embodiment, only for technical conceive of the present invention and feature are described, its object is to person skilled in the art can be understood content of the present invention and implement according to this, can not limit the scope of the invention with this.All equivalences done according to Spirit Essence of the present invention change or modify, and all should be encompassed within protection scope of the present invention.
Claims (4)
1. the method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment, it is characterized in that: select Guangdong cabbage heart conventional variety Lubao 60 tian, SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 and SUZHOUQING(sic) type tender flower stalk inbred line G11 as parent material, at least comprise the following steps:
The first step, with described Guangdong cabbage heart conventional variety Lubao 60 tian for female parent and described SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 are for paternal hybrid, obtains first-filial generation seed 1F1;
Second step, backcrosses for male parent for recurrent parent and described first-filial generation 1F1 with described Lubao 60 tian, obtains the first-filial generation seed BC1F1 of first backcross generation;
3rd step, obtains the second filial generation seed BC1F2 of first backcross generation with the first-filial generation BC1F1 selfing of described first backcross generation;
4th step, the plant selfing selecting plant type compact in the second filial generation BC1F2 of described first backcross generation obtains the hybridization three generations seed BC1F3 of first backcross generation;
5th step, with described SUZHOUQING(sic) type tender flower stalk inbred line G11 for maternal to hybridize with described hybridize three generations BC1F3 obtains first-filial generation seed 2F1, the hybridization four generation seed BC1F4 of the hybridization three generations BC1F3 selfing acquisition first backcross generation of described first backcross generation;
6th step, with described first-filial generation 2F1 for maternal to hybridize with the hybridization four generation BC1F4 of described first backcross generation obtains new first-filial generation seed 3F1;
7th step, the plant selfing selecting plant type compact in described first-filial generation 3F1 obtains second filial generation seed 3F2;
8th step, in described second filial generation 3F2, select that plant type is compact, upright blade, beginning flower time lateral bud not sprout and the plant selfing of a kind of sedge look glossy dark green obtains hybridization three generations seed 3F3, this hybridization three generations seed 3F3 is the upright compact germ plasm resource of tender flower stalk not affected by environment;
The morphological feature of described tender flower stalk upright compact germ plasm resource is that plant type is compact, upright blade, and when beginning to spend, lateral bud is not sprouted, and plant height is 36 ~ 38 centimetres, strain width is 19 ~ 21 centimetres, a kind of sedge look glossy dark green wax powder-free, and main a kind of sedge is high is 23 ~ 25 centimetres, main a kind of sedge is slightly 1.5 ~ 2.5 centimetres, and tender flower stalk weighs 59 ~ 62 grams; Blade is long 24.5 ~ 26 centimetres, and blade is wide 14 ~ 16 centimetres, long 5.5 ~ 7 centimetres of petiole, leaf green is glossy subcircular, a kind of sedge leaf petiole and a kind of sedge angle little, blade face is open and flat little with a kind of sedge angle.
2. the method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment according to claim 1, it is characterized in that: the morphological feature of described Guangdong cabbage heart conventional variety Lubao 60 tian is plant height 26 ~ 28 centimetres, strain 28 ~ 34 centimetres, base leaf blade oval, bottle green, long 17 ~ 19 centimetres of blade, blade is wide 7.5 ~ 9 centimetres, long 5 ~ 6.5 centimetres of petiole, wide 0.9 ~ 1.5 centimetre; A kind of sedge leaf is narrow avette, and tender flower stalk consolidation bar is even, and dark green glossy, main a kind of sedge is high 22 ~ 26 centimetres, and main a kind of sedge transverse diameter 1.5 ~ 1.8 centimetres, tender flower stalk weighs 35 ~ 40 grams.
3. the method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment according to claim 1, it is characterized in that: the morphological feature of described SUZHOUQING(sic) type common Chinese cabbage inbred line Q2 is plant height 17 ~ 18 centimetres, strain 24 ~ 25 centimetres, Weight per plant 278 ~ 283 grams, the number of blade is 20 ~ 22, and property with a tight waist is good, blade subcircular, petiole cochlear, dark green leaf, the green white of petiole; During bolting, a kind of sedge green has draped over one's shoulders wax powder, and tender flower stalk plant type compact half is upright, petiole and interval short, a kind of sedge leaf petiole and a kind of sedge angle medium, blade face is open and flat medium with a kind of sedge angle.
4. the method for creating of the upright compact germ plasm resource of tender flower stalk not affected by environment according to claim 1, is characterized in that: the morphological feature of described SUZHOUQING(sic) type tender flower stalk inbred line G11 is that plant type is compact, plant height 32 ~ 34 centimetres, strain 23 ~ 25 centimetres; There is wax powder on the green surface of a kind of sedge, and tender flower stalk weighs 49 ~ 51 grams, thick 1.5 ~ 2.2 centimetres of a kind of sedge, and a kind of sedge is high 21 ~ 22 centimetres; Leaf look green, blade subcircular, and blade is long 15 ~ 16 centimetres, and blade is wide 9 ~ 11 centimetres, long 5 ~ 6 centimetres of petiole, and petiole is wide 1.5 ~ 2.0 centimetres, a kind of sedge leaf petiole and a kind of sedge angle little, blade face is open and flat large with a kind of sedge angle.
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| CN102986523A (en) * | 2012-11-30 | 2013-03-27 | 江苏太湖地区农业科学研究所 | Breeding method of novel germplasm resource of green tender flower stalk |
| CN104686317A (en) * | 2015-02-04 | 2015-06-10 | 湖南省蔬菜研究所 | Method for breeding multi-bolting cold-resistant flowering cabbage |
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| CN1647615A (en) * | 2005-02-05 | 2005-08-03 | 西北农林科技大学 | Method for bolt cytoplasma male sterility line breeding, reproduction andp reparing hybrid seed |
| CN102986523A (en) * | 2012-11-30 | 2013-03-27 | 江苏太湖地区农业科学研究所 | Breeding method of novel germplasm resource of green tender flower stalk |
| CN104686317A (en) * | 2015-02-04 | 2015-06-10 | 湖南省蔬菜研究所 | Method for breeding multi-bolting cold-resistant flowering cabbage |
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| 张华等: ""菜薹新品种绿宝60天菜心的选育"", 《中国蔬菜》 * |
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