CN109757361A - The method for creating for the pumpkin resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent - Google Patents
The method for creating for the pumpkin resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent Download PDFInfo
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- CN109757361A CN109757361A CN201910213419.8A CN201910213419A CN109757361A CN 109757361 A CN109757361 A CN 109757361A CN 201910213419 A CN201910213419 A CN 201910213419A CN 109757361 A CN109757361 A CN 109757361A
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Abstract
The method for creating of excellent, long cylindricality that the present invention provides a kind of qualities and the excellent pumpkin resource of Comprehensive Traits, the following steps are included: step 1) is chosen, the unlimited long-neck cylinder pumpkin single plant of Comprehensive Traits, quality is female parent and the oval pumpkin single plant that Comprehensive Traits are excellent and quality is excellent is paternal hybrid, obtains F1Generation；Step 2) is by F1Generation selfing obtains F2Generation, and in F2It is that long cylindrical single plant and male parent pumpkin single plant are returned that ovary is chosen in generation, and selection fruit type is long cylindricality, the pumpkin single plant material sowing that quality is excellent and Comprehensive Traits are excellent, obtains BC1F1Generation；Step 3) chooses BC1F1Ovary is the successively selfing in the strain of 3 generations of long cylindrical single plant in generation, 1 generation be in sisters hand over and the strain of 1 generation in selfing obtain excellent stable quality, long cylindricality and the excellent Pumpkin Inbred Lines of Comprehensive Traits.The present invention obtains excellent quality, long cylindricality and the excellent Pumpkin Germplasm resource of Comprehensive Traits for the first time, has filled up the market vacancy.
Description
Technical field
The present invention relates to plant genetics and breeding technical fields, particularly, are related to a kind of excellent quality, long cylindricality and Comprehensive Traits
The method for creating of excellent pumpkin resource.
Background technique
Musky gourd (C.moschata.Duchesne, 2n=40) is one of four cultivars of Cucurbita, and okra fruit is sweet in flavor
Agreeable to the taste, it is one of main melon dish of summer and autumn that old melon is capable of using as feed or coarse cereals, has extensive nutritive value, medical value
And economic value.
With the improvement of living standards, requirement of the people to pumpkin quality is higher and higher.This sweet type in musky gourd
The advantages that pumpkin is high, quality is excellent, disease resistance is strong with its yield is widely cultivated, at present about 400,000 hectares of sown area, Zhan Zhongguo
The 90% of pumpkin commodity cultivated area, and the maximum growing area in the whole nation is formed in Hunan Dongting Lake region, product mainly transports pearl to
The all provinces of triangle and the north, for ensureing that national pumpkin market supply has played important function.Due to this sweet pumpkin parent's first is that
Using the pumpkin resource of long-neck cylinder, fruit shape shows as that top is relatively thin, lower part is expanded.During transportation, this curcumbitate is not only
Wasting space further increases transportation cost, and finally passes on to consumers.And long cylindrical pumpkin compares conducive to putting, and saves
Space reduces transportation cost.In addition, existing market pumpkin mainly with fresh food based on, and be suitable for processing tailored version pumpkin it is less,
Long cylindricality is easy to process due to peeling conducive to machine.The thus excellent long cylindricality pumpkin resource of initiative quality will process class to cultivate
Type pumpkin new varieties establish material foundation.Therefore, the cylindrical pumpkin resource of good length is formulated, and utilizes the breeding of the type resource
Sweet this type pumpkin new varieties high-quality, curcumbitate is more well-balanced have weight for the quality, transport and the processing problems that solve in production
Want meaning.
Summary of the invention
The initiative side of excellent, long cylindricality that it is an object of that present invention to provide a kind of qualities and the excellent pumpkin resource of Comprehensive Traits
Method is mostly long-neck cylindrical shape to solve existing sweet this type pumpkin, and curcumbitate top is relatively thin, lower part is expanded, more wasteful space, increases
Add transportation cost, and is not easy to the technical issues of machine is peeled and processed.
To achieve the above object, excellent, long cylindricality that the present invention provides a kind of qualities and the excellent pumpkin resource of Comprehensive Traits
Method for creating, comprising the following steps:
Step 1), choose Comprehensive Traits, quality be unlimited, pumpkin single plant of long-neck cylinder be it is maternal excellent with Comprehensive Traits and
Excellent, the oval pumpkin single plant of quality is paternal hybrid, obtains F1Generation；
Step 2), by the F1Generation selfing obtains F2Generation；And in F2The long cylindrical single plant of ovary and the male parent are chosen in generation
The backcrossing of pumpkin single plant chooses the long cylindricality of fruit type, the pumpkin single plant material sowing that quality is excellent and Comprehensive Traits are excellent, obtains BC1F1
Generation；
Step 3) chooses BC1F1Ovary is the successively selfing in the strain of 3 generations of the cylindrical single plant of length in generation, and 1 generation was that interior sisters hand over
Excellent stable quality, long cylindricality and the excellent Pumpkin Inbred Lines of Comprehensive Traits are obtained with selfing in the strain of 1 generation.
Further, male parent described in step 1) and female parent are musky gourd.
Further, by F in step 2)1For 5 plants of plant plantations in crop field, built cultivation embroiders selfing acquisition F2Generation；It will
F280 plants of plantations of generation are in crop field, and choosing ovary is that long cylindrical single plant is embroidered and is returned with male parent, and choosing 6 plants of fruit type is long column
The pumpkin single plant material sowing that shape, quality are excellent and Comprehensive Traits are excellent obtains BC1F1Generation.
Further, by BC in step 3)1F1Each 80 plants of plantations of 6 strains of generation choose ovary in crop field as long cylindricality flower
Piece selfing is embroidered, choose that 5 plants of qualities are excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, obtains BC1F2；It will
BC1F2Each 80 plants of plantations of 5 strains in crop field, choosing ovary is that long cylindricality flower embroiders selfing, chooses that 4 plants of qualities are excellent, integrate
Character obtains BC close to male parent or material sowing identical with male parent1F3；By BC1F34 strains it is each 80 plants plantation in big
Field chooses ovary and is that long cylindricality single plant flower embroiders selfing, and 3 plants of qualities of selection are excellent, Comprehensive Traits are close to male parent or and father
This identical material sowing, obtains BC1F4；By BC1F4Each 80 plants of plantations of 3 strains in crop field, selections ovary is long cylindricality list
It is that interior sisters hand over that strain flower, which embroiders, chooses that 1 plant of quality is excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent,
Obtain BC1F5；By BC1F580 plants of 1 strain plantation in crop field, and choose ovary be long cylindricality single plant flower embroider selfing, choosing
Take that 1 plant of quality is excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, i.e., acquisition quality is excellent, long cylindricality and comprehensive
Close the excellent Pumpkin Germplasm material of character.
Further, the Comprehensive Traits include growth potential, disease resistance and yielding ability.
Further, the Comprehensive Traits are excellent refers to that growth potential is strong；Seedling stage indoors artificial inoculated identification powder mildew resistance
Index is less than 45；Per mu yield 3000kg or more.
Further, the excellent finger soluble solid content of the quality is 9.5% or more；Delicate mouthfeel, powder sweet tea.
Further, choosing the pumpkin that ovary is long cylindricality single plant is that ovary shape determines before not pollinated according to flower
's.
The invention has the following advantages:
1, the method for creating for the pumpkin resource that quality provided by the invention is excellent, long cylindricality and Comprehensive Traits are excellent obtains for the first time
The Pumpkin Germplasm resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent, has filled up the market vacancy.
2, compared with traditional pumpkin resource material selection, by by maternal quality, Comprehensive Traits in the method for the present invention
Unlimited long-neck cylindrical shape pumpkin with male parent quality is excellent, oval and Comprehensive Traits excellent material hybridizes, separate, be returned, separate,
It is selfed and is that interior sisters hand over purifying, the favorable genes of male parent quality and the excellent pumpkin of Comprehensive Traits are imported into maternal pumpkin material.
3, the method for creating for the pumpkin resource that a kind of quality provided by the invention is excellent, long cylindricality and Comprehensive Traits are excellent utilizes
Ovary shape carries out the pollination of selectivity before blooming, and shortens breeding process, alleviates workload, improves the selection of the character
Efficiency.
4, the method for creating for the pumpkin resource that a kind of quality provided by the invention is excellent, long cylindricality and Comprehensive Traits are excellent is in material
It is handed over when material purifying using sisters in 1 generation system, effectively prevents the hair of conventional self-mating system initiative inbreeding depression phenomenon after being selfed for 3 generations
It is raw.
5, excellent using high-quality, the long cylindrical pumpkin Comprehensive Traits of the method for the present invention breeding, show as delicate mouthfeel, powder
Sweet tea, growth potential, disease-resistant resistance are strong, and the long cylindricality of fruit type is readily transported, and have preferable commodity.The present invention is to meet market need
Want, formulate it is high-quality, be readily transported and pumpkin material that Comprehensive Traits are excellent provides a kind of simple, effective method for creating.
Other than objects, features and advantages described above, there are also other objects, features and advantages by the present invention.
Below with reference to figure, the present invention is described in further detail.
Detailed description of the invention
It constitutes part of this application attached drawing to be used to provide further understanding of the present invention, schematic implementation of the invention
Example and its explanation are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings:
Fig. 1 is the method for creating flow diagram of the preferred embodiment of the present invention；
Fig. 2 is that Comprehensive Traits are common, quality is general in the present invention, the cylinder-shaped pumpkin resource material 137 (female parent) of long-neck is shone
Piece；
Fig. 3 is pumpkin resource material 182 (male parent) photo that Comprehensive Traits are excellent, quality is excellent in the present invention, oval；
Fig. 4 is that (Comprehensive Traits are excellent, quality is excellent, long for the excellent pumpkin resource material formulated in the embodiment of the present invention
Cylindricality) 95-4-3-3-2-1 (product melon) appearance photo.
Specific embodiment
The embodiment of the present invention is described in detail below in conjunction with attached drawing, but the present invention can be limited according to claim
Fixed and covering multitude of different ways is implemented.
Hybridization: the combination of gynoecium and stamen between the plant of the Seed Development of two different pumpkins.
Selfing: the combination of the female and male gametophyte from same individual, i.e., the combination of the gynoecium and stamen of same plant.
Backcrossing: filial generation (including first filial generation or son mostly generation) hybridizes, this side with any one parent in two parents
Method is called backcrossing.
Sisters hand over: different in same strain (plant that the more seeds that the same pumpkin generates grow up to belongs to same strain)
The combination of gynoecium and stamen between plant.
Embodiment 1
Step 1), hybridization: choosing Inst. of Vegetables, Hunan Prov.'s breeding high, (Comprehensive Traits are common, quality for self-mating system 137
General long-neck is cylinder-shaped) it is female parent, with Inst. of Vegetables, Hunan Prov.'s breeding high, for self-mating system 182, (Comprehensive Traits are excellent, quality
It is excellent, oval) it is male parent, hybridization obtains F1Generation.Gained F1For pumpkin Comprehensive Traits substantially between male parent and female parent.F1Generation
Phenotype it is consistent.Specifically, the Comprehensive Traits include growth potential, disease resistance and yielding ability.Comprehensive Traits are excellent to refer to life
Growing way is strong；Seedling stage, indoors artificial inoculated identification powder mildew resistance index was less than 45；Per mu yield 3000kg or more.The quality is excellent main
Refer to soluble solid content height 9.5% or more；Delicate mouthfeel, powder sweet tea.
Step 2), selfing and backcrossing: by F1For 5 plants of plant plantations in crop field, built cultivation embroiders selfing acquisition F2Generation；It will
F280 plants of plantations of generation are in crop field, to F2Ovary form is observed when generation blooms as it can be seen that F2For plant, there are trait segregations, select ovary
It is embroidered and common pumpkin single plant (male parent 182) backcrossing of excellent carpopodium, selection synthesis close to long cylindricality or for long cylindrical single plant
Character obtains BC close to male parent or pumpkin single plant material sowing identical with male parent1F1Generation.
Step 3), selfing three generations, sister hands over and selfing: choosing BC1F1Each 80 plants of plantations of 6 strains of generation are chosen in crop field
Ovary is that long cylindrical single plant flower embroiders selfing, chooses that 5 plants of qualities are excellent, Comprehensive Traits are close to male parent or identical with male parent
Material sowing obtains BC1F2；By BC1F2Each 80 plants of plantations of 5 strains in crop field, choose ovary and be connected in long cylindrical single plant flower
Piece selfing is embroidered, choose that 4 plants of qualities are excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, obtains BC1F3；It will
BC1F3Each 80 plants of plantations of 4 strains in crop field, choosing ovary is that long cylindrical single plant flower embroiders selfing, chooses 3 plants of qualities
Excellent, Comprehensive Traits obtain BC close to male parent or material sowing identical with male parent1F4；By BC1F4Each 80 plants of kinds of 3 strains
Yu great Tian is planted, it is that interior sisters hand over that choose ovary, which be that long cylindrical single plant flower embroiders, chooses that 1 plant of quality is excellent, Comprehensive Traits are close
Male parent or material sowing identical with male parent obtain BC1F5；By BC1F580 plants of 1 strain plantations in crop field, and choose son
Room is that long cylindrical single plant flower embroiders selfing, chooses that 1 plant of quality is excellent, Comprehensive Traits are close to male parent or material identical with male parent
Expect sowing, that is, obtains high-quality, long cylindricality and the excellent material 95-4-3-3-2-1 (product melon) of Comprehensive Traits.
The quantity of plantation in step 2) and step 3) is all not limited only to the data, if but plantation strain number it is very few, deposit
It will affect the too small limitation for influencing efficiency of selection of genetic breeding and group in the death of individual plant, and when plantation amount is excessive, is obvious
It is greatest waste.
In addition, in step 3) of the present invention, for BC1F1Character settle out, first carry out the selfing of 3 generations.Although theoretically certainly
It is purer to hand over the more a variety of matter of number, but algebra not only wastes time too much, money, and there are germplasm degradation phenomena in pumpkin, it is excellent
Selected from explanation number 3-6 instead of between；The too low then seed properties of selfing algebra are impure, and character is inconsistent between strain and strain.And
In third step of the invention, to avoid genetic devolution, sisters in generation system need to be carried out and handed over.Specifically: successively in the strain of 3 generations certainly
It hands over, sisters hand in 1 generation system and the interior selfing of 1 generation strain is best mode.
Breeding by the method for the invention obtains that quality is excellent, long cylindrical material 95-4-3-3-2-1, and 2018 in Hainan kind
It plants, shows as late-maturing, 26 section of the first female flower section, single long 31.5cm of melon, the wide 11.2cm of fruit, pulp thickness 3.1cm, single melon weight
2.5kg, soluble solid content 10.5%.
For the present invention using the height of Inst. of Vegetables, Hunan Prov.'s breeding for self-mating system 182, which shows as fruit type ellipse
Shape, quality are excellent, and growth potential is strong, disease resistance is strong.The self-mating system is high-quality, long cylindricality and Comprehensive Traits good resource material in the present invention
Material foundation has been established in the initiative of material.
The present invention also using the shape selection pollination single plant of ovary before blooming, shortens breeding process, improves selection effect
Rate.Traditional pedigree seed selection needs inbreeding of more generation, and there are serious germplasm degradation phenomena, is difficult to select Comprehensive Traits excellent
Breeding material；The present invention is handed over using sisters in backcrossing and generation system, effectively prevents the generation of the phenomenon.The long cylindricality of breeding
Pumpkin material, it is the fruit type breeding of pumpkin difference that quality and Comprehensive Traits, which have reached the level of the excellent pumpkin material of character,
Material initiative provides a kind of simple effective method.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of method for creating for the pumpkin resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent, which is characterized in that including following
Step:
Step 1) chooses that Comprehensive Traits, quality be unlimited, pumpkin single plant of long-neck cylinder is maternal and quality excellent with Comprehensive Traits
Excellent, oval pumpkin single plant is paternal hybrid, obtains F1Generation；
Step 2), by the F1Generation selfing obtains F2Generation；And in F2The long cylindrical single plant of ovary and the male parent pumpkin are chosen in generation
Single plant backcrossing chooses the long cylindricality of fruit type, the pumpkin single plant material sowing that quality is excellent and Comprehensive Traits are excellent, obtains BC1F1Generation；
Step 3) chooses BC1F1Ovary is the cylindrical single plant of length successively through the interior selfing of 3 generations strain in generation, and 1 generation was that interior sisters hand over and 1 generation
Selfing obtains excellent stable quality, long cylindricality and the excellent Pumpkin Inbred Lines of Comprehensive Traits in strain.
2. the method for creating for the pumpkin resource that quality according to claim 1 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is that male parent described in step 1) and female parent are musky gourd.
3. the method for creating for the pumpkin resource that quality according to claim 1 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is, by F in step 2)1For 5 plants of plant plantations in crop field, built cultivation embroiders selfing acquisition F2Generation；By F280 plants of kinds of generation
Plant Yu great Tian, choosing ovary is that long cylindrical single plant is embroidered and is returned with male parent, choose 6 plants of fruit type be long cylindricality, quality it is excellent and
The excellent pumpkin single plant material sowing of Comprehensive Traits obtains BC1F1Generation.
4. the method for creating for the pumpkin resource that quality according to claim 1 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is, by BC in step 3)1F1Each 80 plants of plantations of 6 strains of generation are in crop field, and choosing ovary is that long cylindricality flower embroiders certainly
It hands over, chooses that 5 plants of qualities are excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, obtain BC1F2；By BC1F25
For each 80 plants of plantations of a strain in crop field, choosing ovary is that long cylindricality flower embroiders selfing, chooses that 4 plants of qualities are excellent, Comprehensive Traits connect
Nearly male parent or material sowing identical with male parent obtain BC1F3；By BC1F3Each 80 plants of plantations of 4 strains in crop field, choose
Ovary is that long cylindricality single plant flower embroiders selfing, and 3 plants of qualities of selection are excellent, Comprehensive Traits are close to male parent or identical with male parent
Material sowing obtains BC1F4；By BC1F4Each 80 plants of plantations of 3 strains in crop field, choosing ovary is that long cylindricality single plant flower is pricked
Flower be in sisters hand over, choose that 1 plant of quality is excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, obtain BC1F5；
By BC1F580 plants of 1 strain plantation in crop field, and choose ovary be long cylindricality single plant flower embroider selfing, choose 1 plant of quality
Excellent, Comprehensive Traits are close to male parent or material sowing identical with male parent, i.e., acquisition quality is excellent, long cylindricality and Comprehensive Traits are excellent
Pumpkin Germplasm material.
5. the method for creating for the pumpkin resource that quality according to claim 1 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is that the Comprehensive Traits include growth potential, disease resistance and yielding ability.
6. the method for creating for the pumpkin resource that quality according to claim 5 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is that the Comprehensive Traits are excellent to refer to that growth potential is strong；Seedling stage, indoors artificial inoculated identification powder mildew resistance index was less than 45；
Per mu yield 3000kg or more.
7. the method for creating for the pumpkin resource that quality according to claim 1 is excellent, long cylindricality and Comprehensive Traits are excellent, special
Sign is that the excellent finger soluble solid content height of quality is 9.5% or more；Delicate mouthfeel, powder sweet tea.
The initiative side for the pumpkin resource that 8. -7 any qualities are excellent according to claim 1, long cylindricality and Comprehensive Traits are excellent
Method, which is characterized in that choosing the pumpkin that ovary is long cylindricality single plant is that ovary shape determines before not pollinated according to flower.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910213419.8A CN109757361A (en) | 2019-03-20 | 2019-03-20 | The method for creating for the pumpkin resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910213419.8A CN109757361A (en) | 2019-03-20 | 2019-03-20 | The method for creating for the pumpkin resource that quality is excellent, long cylindricality and Comprehensive Traits are excellent |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114097607A (en) * | 2021-12-24 | 2022-03-01 | 苏州市农业科学院 | Cross breeding method of long cylindrical orange melons |
| CN114391473A (en) * | 2022-02-15 | 2022-04-26 | 湖南省蔬菜研究所 | Pumpkin inbred line creation method with white flower mark and excellent comprehensive characters |
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| CN103229714A (en) * | 2013-05-28 | 2013-08-07 | 万农高科集团有限公司 | Novel cultivating method of hybrid seeds of sweet pumpkin |
| CN106962175A (en) * | 2017-03-15 | 2017-07-21 | 湖南省蔬菜研究所 | The method for creating of carpopodium impact resistance and the excellent Pumpkin Germplasm resource of Comprehensive Traits |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103229714A (en) * | 2013-05-28 | 2013-08-07 | 万农高科集团有限公司 | Novel cultivating method of hybrid seeds of sweet pumpkin |
| CN106962175A (en) * | 2017-03-15 | 2017-07-21 | 湖南省蔬菜研究所 | The method for creating of carpopodium impact resistance and the excellent Pumpkin Germplasm resource of Comprehensive Traits |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114097607A (en) * | 2021-12-24 | 2022-03-01 | 苏州市农业科学院 | Cross breeding method of long cylindrical orange melons |
| CN114391473A (en) * | 2022-02-15 | 2022-04-26 | 湖南省蔬菜研究所 | Pumpkin inbred line creation method with white flower mark and excellent comprehensive characters |
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Application publication date: 20190517 |