CN101213934A - Single clone choiceness F1 stevia rebaudianum hybrid seeds production method - Google Patents
Single clone choiceness F1 stevia rebaudianum hybrid seeds production method Download PDFInfo
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- CN101213934A CN101213934A CNA2008100193899A CN200810019389A CN101213934A CN 101213934 A CN101213934 A CN 101213934A CN A2008100193899 A CNA2008100193899 A CN A2008100193899A CN 200810019389 A CN200810019389 A CN 200810019389A CN 101213934 A CN101213934 A CN 101213934A
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Abstract
The present invention relates to a Stevia rebaudianum sugar crop mono-clone excellent F1 generation seed-preparation technological method. The stevia rebaudianum excellent F1 generation seed-preparation technology mainly comprises that the selection of the parent strain for the seed preparation of stevia rebaudianum F1 generation: one clone is selected as the female parent and one F1 generation that is hybrid of two clones as the male parent; through the fixed plantation of the special parent strain, the male parent and the female parent are ensured to be planted in the same furrow and to be inter-planted with the male parent and the female parent at the two adjacent furrows; and the method for collecting the seed by swinging the branches and the one-off seed collection method are adopted respectively. The present invention provides a fundamental effective method and insurance for the northern region to improve the quality of the raw material of the stevia rebaudianum and to supply the source for the excellent F1 generation stevia rebaudianum seed, and provides possibility for accelerating and promoting the upgrading of the excellent species of the stevia rebaudianum in northern region, which is a sugar cash crop seed-preparation technology with high application and popularization value.
Description
Technical field
The present invention relates to elite seed breeding and the production method of sugar [yielding stevia rebaudianum, belong to plant artificial propagation and cultivation method technical field.
Background technology
Stevia rebaudianum Stevia rebaudiana Bertoni. belongs to the composite family herbaceos perennial, areas such as wild naturally Brazil, Paraguay in South America.Be known as Paraguay's beet in the original producton location, the Brazilian is the sugar grass.It is a kind of new sugar [yielding that after beet, sugarcane, develops into.
Stevia rebaudianum is introduced China the seventies in last century, and cultivation practices for many years shows that stevia rebaudianum has adaptability widely, all can cultivate in different weather conditions areas such as China northeast, northwest, North China, East China, southwest.But, therefore,, be the key of effectively guaranteeing at different good stevia rebaudianum kinds in weather conditions cultivation area of China and cured leaf material quality according to the modes of reproduction of the suitable stevia rebaudianum kind of different weather conditions selections because stevia rebaudianum is the short-day plant of cold resistance difference.China is still maximum in the world stevia rebaudianum primary producing country at present, and area under cultivation ratio southern, northern stevia rebaudianum is about 2.5: 4.Southern area selects for use the stevia rebaudianum improved seeds can guarantee the kind of stevia rebaudianum improved seeds and the quality of stevia rebaudianum cured leaf raw material by the mode of asexual cuttage and division propagation preferably.Northern area is because the cold suitable modess of reproduction such as asexual cuttage and plant division that adopt of winter climate, can only select the stevia rebaudianum cultivation modes of reproduction of sexual seminal propagation for use, and the seed of the usefulness of northern area cultivation at present, almost be through originate from numerous accessory seed certainly for years, because the moral character of these accessory seeds is degenerated year by year, naturally produced cured leaf material quality also descends year by year.Therefore, solving the quality key issue of northern area stevia rebaudianum cured leaf raw material, is must change the continuous subculture that uses from originating from seed that numerous mixed intergrowth produces as using high-quality F every year
1For seed, and solve high-quality F
1For the seeding technique problem that seed is produced, be only the key of the quality that solves northern area stevia rebaudianum cured leaf raw material.
The good F of single clone
1As follows for the brief introduction of stevia rebaudianum seeding technique method:
The stevia rebaudianum seed of northern China use at present, it mainly is the hybrid seed that originates from numerous mixed friendship certainly that the area, Shandong produces, because these seeds are for originating from numerous mixed friendship seed that mingles for many years continuously certainly, the average quality of seed is serious degradation and continuation reduction year by year, therefore, the feasible quality of the stevia rebaudianum cured leaf raw material of this seed production that uses seriously descends.In order to change this situation, must set up the good F of long-term, stable stevia rebaudianum
1For production of hybrid seeds base, and good stevia rebaudianum provenance and good F
1For the seeding technique method is to carry out stevia rebaudianum F
1The necessary condition of the production of hybrid seeds.
The invention solves good stevia rebaudianum provenance and good F
1For the seeding technique method, solved high-quality F
1Seeding technique for seed production.
Summary of the invention: the good F of single clone
1For the stevia rebaudianum seeding technique.
The good F of single clone
1As male parent, No. 3, middle mountain and No. 4 first familiar generations in middle mountain are as female parent for No. 3, mountain in selecting in the stevia rebaudianum seeding technique; No. 4, mountain is as male parent in the selection, and No. 3, middle mountain and No. 4 first familiar generations in middle mountain are as female parent; No. 1 clone in mountain is as male parent in the selection stevia rebaudianum improved seeds, and F is hybridized No. 4 in No. 3, middle mountain and middle mountain
1Generation is as maternal, and when production of hybrid seeds parent field planting, must guarantee in the same furrow male parent, female parent be respectively row and with about two furrow close on capable father, this alternate planting of mother.Stevia rebaudianum F
1All select the cultivated land dry land at nearly water source for production of hybrid seeds milpa.Father, this setting date of mother be in the first tenday period of a month in late May to June, stevia rebaudianum the full-bloom stage in area, the middle and lower reach of Yangtze River late September to early October, the maturing stage of seed in 10 months, the last ten-days period.Seed results are carried out several times earlier according to the seed maturity situation and are shaken branch and receive kind, carry out disposable results after treating plant the flowers are in blossom 85% or more.
The good F of single clone
1For the concrete grammar of stevia rebaudianum seeding technique with require as follows:
1, the good F of single clone
1Selection for stevia rebaudianum seeding technique parent:
The good F of single clone
1Select the hybrid strain combo for the stevia rebaudianum seeding technique according to the different material quality requirements, No. 3, mountain was as male parent during the present invention selected, and No. 3, middle mountain and No. 4 first familiar generations in middle mountain are as female parent; No. 4, mountain is as male parent in the selection, and No. 3, middle mountain and No. 4 first familiar generations in middle mountain are as female parent; No. 1 clone in mountain is as male parent in the selection stevia rebaudianum improved seeds, and F is hybridized No. 4 in No. 3, middle mountain and middle mountain
1In generation, is as maternal.
2, the whole ground and the requirement of planting
The good F of single clone
1Put in order the ground and the requirement of planting for the stevia rebaudianum seeding technique: selecting standby cultivated land dry land, is wide 120cm and high 20cm with the direct bedding in common farmland, furrow spacing 40cm.Plant triplex row on the wide furrow of 120cm, respectively apart from furrow edge 20cm, line-spacing is 40cm, and spacing in the rows is 20cm; The father, maternal be the alternate plantation of row, must guarantee these furrow or with about two furrow close on capable father, maternal alternate planting.
3, cultivation management requirement
Whole ground, the area in annual 1 season was finished before the December in every year, and whole ground, the area in annual two seasons was finished before April 30.Impose a small amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.
The good F of single clone
1For the setting date of stevia rebaudianum seeding technique plant in the late May or the first tenday period of a month in June, field planting seedling plant height 10cm-15cm, and 3 pairs to 5 pairs blades of tool, water permeable after the field planting, multiple permeable 1～2 time of next day, seedling-slowing stage 5 days watered 1 time in per afterwards 3～7 days, chased after composite fertilizer 1 time by every mu of 25kg before florescence, the field management of regularly hoeing up weeds.
4, seed results and cleaning
Stevia rebaudianum the full-bloom stage in area, the middle and lower reach of Yangtze River about late September to early October, the maturing stage of seed in 10 months, the last ten-days period.Owing to be subjected to plant blossom influence successively, the maturing stage of plant seed is not quite identical, in addition, behind the seed maturity under the sunny situation of dry weather, automatically be scattered easily, therefore, the branch receipts of shaking that the results of seed are carried out several times earlier according to the mature condition of seed are planted, and generally have approximately plant and carry out disposable results after the flowers are in blossom more than 85%.
Shake the concrete grammar that branch is received kind: mid-October the plant part seed maturity early stage, select sunny weather, will the sprig of part mature seed be arranged on the plant, be inclined to the container or the pocket jog of standby suitable size, to collect the seed that maturation is scattered.
The concrete grammar of disposable results: in late October, plant generally has after the flowers are in blossom more than 85% approximately, the cool a few days after ripening seed that shines in rearmounted cool threshing ground is gathered in the whole strain of plant be scattered naturally.Removing straw gets final product.
The cleaning of seed: the seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and fine impurities respectively keeping away cool solarization the under the high light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
Embodiment:
Embodiment 1
The good F of single clone
1For the stevia rebaudianum seeding technique: select in the stevia rebaudianum improved seeds No. 3 clones in mountain as female parent; No. 4 hybridization in No. 3, middle mountain and middle mountain F
1In generation, is as male parent.
1, whole ground bedding: select standby cultivated land dry land, impose a small amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.With the direct bedding in common farmland is wide 120cm and high 20cm, furrow spacing 40cm.
2, field planting and field management: 3 pairs of blades of field planting seedling, plant height 10cm, stevia rebaudianum F
1For production of hybrid seeds parent's setting date May 22, plantation 3 row on the wide furrow of every 120cm, respectively apart from furrow edge 20cm, line-spacing is 40cm, spacing in the rows is 20cm; Every ridge cultivation 3 row, male parent and maternal alternate plantation, and guarantee and about two furrow alternate the planting of closing on capable father, female parent.Water permeablely after the field planting, multiple permeable 2 times of next day, seedling-slowing stage 5 days watered 1 time after 5 days, and by every mu 25kg topdress 1 time between seedling an August 11 moon florescence before, field management such as in time hoe up weeds, loosen the soil.
3, results seed:
Production of hybrid seeds parent's full-bloom stage was September 20 to October 10, seed is since maturation successively on October 12, have only the branch receipts of shaking early stage 12-18 day in October of a small amount of maturation to plant at seed, to the sprig of part mature seed be arranged on the plant, be inclined to the container or the pocket jog of standby suitable size, to collect the seed that maturation is scattered.
October 20 plant have generally that the flowers are in blossom more than 85% and carry out disposable results seed later.The seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and fine impurities respectively keeping away cool solarization the under the high light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
Embodiment 2
The good F of single clone
1For the stevia rebaudianum seeding technique: select in the stevia rebaudianum improved seeds No. 4 clones in mountain as female parent; No. 4 hybridization in No. 3, middle mountain and middle mountain F
1In generation, is as male parent.
1, whole ground bedding: select standby cultivated land dry land, impose a small amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.With the direct bedding in common farmland is wide 120cm and high 20cm, furrow spacing 40cm.
2, field planting and field management: 4 pairs of blades of field planting seedling, plant height 12cm, stevia rebaudianum F
1For production of hybrid seeds parent's setting date May 24, plantation 3 row on the wide furrow of every 120cm, respectively apart from furrow edge 20cm, line-spacing is 40cm, spacing in the rows is 20cm; Every ridge cultivation 3 row, this alternate plantation of father and mother, and guarantee with about two furrow alternate the planting of closing on capable father, female parent.Water permeablely after the field planting, multiple permeable 2 times of next day, seedling-slowing stage 5 days watered 1 time after 6 days, and by every mu 26 kg topdress 1 time between seedling an August 16 moon florescence before, field management such as in time hoe up weeds, loosen the soil.
3, results seed: production of hybrid seeds parent's full-bloom stage is about September 25 to October 15, seed is since maturation successively on October 19, have only the early stage of a small amount of maturation at seed, shaking the branch receipts plants, to the sprig of part mature seed be arranged on the plant, be inclined to the container or the pocket jog of standby suitable size, to collect the seed that maturation is scattered.
October 23 plant have approximately generally that the flowers are in blossom more than 85% and carry out disposable results later and planted.The seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and fine impurities respectively keeping away cool solarization the under the high light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
Embodiment 3
The good F of single clone
1For the stevia rebaudianum seeding technique: select in the stevia rebaudianum improved seeds No. 1 clone in mountain as female parent, No. 4 hybridization in No. 3, middle mountain and middle mountain F
1In generation, is as male parent.
1, whole ground bedding: select standby cultivated land dry land, impose a small amount of fertilizer or N, P, every mu of 50kg of K composite fertilizer before the whole ground as base manure.With the direct bedding in common farmland is wide 120cm and high 20cm, furrow spacing 40cm.
2, field planting and field management: 5 pairs of blades of field planting seedling, plant height 15cm, stevia rebaudianum F
1For production of hybrid seeds parent's setting date in May 20, plantation 2 row on the wide furrow of every 120cm, respectively apart from furrow edge 20cm, line-spacing is 80cm, spacing in the rows is 15cm; It is capable that father and mother originally are respectively, and guarantee and about two furrow close on capable father, maternal alternate planting.Water permeablely after the field planting, multiple permeable 2 times of next day, seedling-slowing stage 5 days watered 1 time in 7 days, and by every mu 25kg topdress 1 time between seedling an August 15 moon florescence before, field management such as in time hoe up weeds, loosen the soil.
3, results seed: production of hybrid seeds parent's full-bloom stage is in September 25 to October 18, seed is since maturation successively on October 20, have only the early stage of a small amount of maturation at seed, shaking the branch receipts plants, to the sprig of part mature seed be arranged on the plant, be inclined to the container or the pocket jog of standby suitable size, to collect the seed that maturation is scattered.
October 25 plant have generally that the flowers are in blossom more than 85% and carry out disposable results seed later.The seed of results uses the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove branches and leaves and fine impurities respectively keeping away cool solarization the under the high light afterwards, and the preservation of dress sealed bag prevents to make moist mildew and rot.
Claims (7)
1. the good F of single clone
1For the stevia rebaudianum producing method for seed, it is characterized in that the good F of single clone
1As male parent, No. 4 clone first familiar generations of No. 3 clones in middle mountain and middle mountain are as female parent for No. 3 clones in mountain in selecting in the stevia rebaudianum seeding technique; No. 4 clones in mountain are as male parent in perhaps selecting, and No. 4 clone first familiar generations of No. 3 clones in middle mountain and middle mountain are as female parent, and No. 1 clone in mountain is as male parent in perhaps selecting, and No. 4 clones in No. 3 clones in middle mountain and middle mountain are hybridized F
1Generation is as maternal, and when production of hybrid seeds parent field planting, must guarantee in the same furrow male parent, female parent be respectively row and with about two furrow close on capable father, this alternate planting of mother, stevia rebaudianum F
1All select the cultivated land dry land at nearly water source for production of hybrid seeds milpa, father, this setting date of mother are in the first tenday period of a month in late May to June, stevia rebaudianum the full-bloom stage in area, the middle and lower reach of Yangtze River late September to early October, the maturing stage of seed generally in 10 months, the last ten-days period, seed results can be carried out several times earlier according to the seed maturity situation and shaken branch and receive kind, carry out disposable results after treating plant the flowers are in blossom 85% or more.
2. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: it is characterized in that 1 is possessed of good qualities and the clone of good economical character and 1 are possessed of good qualities by 2 and the F of the clone hybridization of good economical character
1On behalf of the parent.
3. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: the whole ground that it is characterized in that production of hybrid seeds milpa was finished in field planting at least in preceding 30 days, imposed a small amount of fertilizer and N, P, every mu of 50Kg of K composite fertilizer before the whole ground as base manure.With the direct bedding in common farmland, wide 120cm and high 20cm, furrow spacing 40cm plants triplex row on the wide furrow of 120cm, and respectively apart from furrow edge 20cm, line-spacing is 40cm, and spacing in the rows is 20cm.
4. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: it is characterized in that stevia rebaudianum F
1For production of hybrid seeds parent's setting date in the first tenday period of a month in late May to June, field planting seedling plant height at 10cm to 13cm, and 3 pairs to 5 pairs blades of tool.
5. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: it is characterized in that on the plant at early stage ripe seed mid-October, selecting sunny weather to shake the branch receipts earlier plants, to the sprig of part mature seed be arranged on the plant, be inclined to the container or the pocket jog of standby suitable size, to collect the seed that maturation is scattered.
6. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: it is characterized in that treating that plant generally has after the flowers are in blossom more than 85% approximately, disposable results seed gathers in the cool a few days after ripening seed that shines in rearmounted cool threshing ground with the whole strain of plant and is scattered naturally.
7. the good F of single clone according to claim 1
1For the stevia rebaudianum producing method for seed: it is characterized in that the seed of gathering in the crops keeping away cool solarization the under the high light, use the scalping of 0.5 * 0.5cm and 0.15 * 0.15cm and dusting cover to remove the cleaning that branches and leaves and fine impurities are carried out seed afterwards respectively, the dress sealed bag preserve prevent to make moist mildew and rot.
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| CNA2008100193899A CN101213934A (en) | 2008-01-08 | 2008-01-08 | Single clone choiceness F1 stevia rebaudianum hybrid seeds production method |
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| CNA2008100193899A CN101213934A (en) | 2008-01-08 | 2008-01-08 | Single clone choiceness F1 stevia rebaudianum hybrid seeds production method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715074A (en) * | 2011-11-01 | 2012-10-10 | 江苏省中国科学院植物研究所 | Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds |
| CN102823386A (en) * | 2012-09-20 | 2012-12-19 | 江苏省中国科学院植物研究所 | Efficient recovery method of stevia seeds |
| CN105104169A (en) * | 2015-08-23 | 2015-12-02 | 林平 | Mating method for stevia rebaudiana hybrid seed production |
-
2008
- 2008-01-08 CN CNA2008100193899A patent/CN101213934A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715074A (en) * | 2011-11-01 | 2012-10-10 | 江苏省中国科学院植物研究所 | Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds |
| CN102823386A (en) * | 2012-09-20 | 2012-12-19 | 江苏省中国科学院植物研究所 | Efficient recovery method of stevia seeds |
| CN105104169A (en) * | 2015-08-23 | 2015-12-02 | 林平 | Mating method for stevia rebaudiana hybrid seed production |
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Open date: 20080709 |