CN1327720A - Method for breeding hybridized seed of stevia rebaudinum - Google Patents
Method for breeding hybridized seed of stevia rebaudinum Download PDFInfo
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- CN1327720A CN1327720A CN 01115131 CN01115131A CN1327720A CN 1327720 A CN1327720 A CN 1327720A CN 01115131 CN01115131 CN 01115131 CN 01115131 A CN01115131 A CN 01115131A CN 1327720 A CN1327720 A CN 1327720A
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Abstract
A method for breeding hybridized seed of stevia rebaudinum includes hybridizing between the stevia rebaudinum cutting as female parent and sexual stevia rebaudinum as male parent and collecting the seeds from stevia rebaudinum cutting. Its advantages are low cost, high content of rebaudioside A and high output of stevioside.
Description
Technical field
A kind of breeding method of stevia hybrid seed, particularly a kind of method of cultivating the stevia hybrid seed of hybridizing with cuttage stevia kind and sexual stevia kind.
Background technology
Stevia is to produce the raw material of stevioside, stevioside be from the stevia leaf, extract by Stevioside (Stevioside), stevia rebaudianum disaccharide glucoside A (Rebaudioside A), the mixture that stevia rebaudianum disaccharide glucoside C (RebaudiosideC) and euonymin A (Dulcoside A) form, the main component of stevioside is stevia rebaudianum disaccharide glucoside A and Stevioside.The sugariness of stevioside is 200-400 a times of sucrose, and heat only is 1/300 of a sucrose, can be widely used in industries such as beverage, food, dairy products, can, flavouring, drinks, cosmetics, pharmacy, so the plantation stevia has vast market prospect.The main quality of stevioside is by Stevioside and stevia rebaudianum disaccharide glucoside A decision, wherein the content of stevia rebaudianum disaccharide glucoside A is high more, the taste of stevioside is high more, quality is good more, and as the quality of the stevia kind of the raw material of stevioside, directly influence output, quality and the production cost of stevioside, so the stevia kind plays a part to the production of stevioside and important.Multiple stevia kind is arranged in the existing market, wherein the overwhelming majority is sexual old kind, sexual old kind is by self breeding and propagation, because the old variety and quality of sexual stevia is constantly degenerated, stevia rebaudianum disaccharide glucoside A content is low in the stevioside, only account for 20% of stevioside gross weight, this kind is produced the stevioside poor quality; Also has a kind of cuttage stevia kind on the market, though cuttage kind stevia rebaudianum disaccharide glucoside A content is about 60% of stevioside gross weight, but because cuttage kind male sterile, himself can not pass through breeding and propagation, can only pass through cottage propagation, and the bothersome effort of cottage propagation, the cuttage and seedling culture cost reaches about 400 yuan for every mu, the seedling cost height, and because the cuttage perennial root of passing the winter is subjected to weather effect big, the survival rate instability, be difficult for to promote, and the output of above-mentioned two kinds of stevia cured leafs is 150～200 kilograms every mu that to account for the cured leaf gross weight be 10% to stevioside content in the cured leaf, 15～20 kilograms of stevioside per mu yields yield poorly.
Summary of the invention
The invention provides a kind of breeding method of hybridizing the stevia seed, this method is hybridized by selecting the stevia kind, obtains the stevia hybrid seed.After the stevia hybrid seed plantation that this method is cultivated, the stevia kind that is obtained, the stevioside output height that extracts in the cured leaf, stevia rebaudianum disaccharide glucoside A content is higher, quality better, this stevia kind is easy to promote.
The present invention further provides a kind of breeding method of hybridizing the stevia seed, utilize this method, during stevia hybridization, hybrid seed knot kind of rate height, the results hybrid seed is convenient, hybrid seed output height.
For obtaining the stevia hybrid seed, the present invention selects cuttage stevia kind and sexual stevia kind to hybridize.
Concrete technical scheme of the present invention is, sexual stevia kind is as male parent in the optional market, cuttage stevia kind is as female parent in the optional market, for guaranteeing between cuttage stevia blooming stage and overlapping between sexual stevia blooming stage, the cuttage stevia can be fertilized, obtain hybridizing the stevia seed, the seedling that partition cuttage stevia perennial root is obtained and sexual stevia seed interval 15 days mix plantation with interior, when treating its flowering, hybridize by the pollen transmission.Because the plant of sexual stevia kind and cuttage stevia kind can be distinguished by outward appearance, or at the planting location of plantation tense marker cuttage kind, treat knot kind after, collection cuttage stevia seed that kind is produced.
Because the male sterile of cuttage stevia kind, so after plantation hybridization knot is planted,, all be hybridization stevia seed from the seed that cuttage stevia kind is collected; The stevia Hybrid that plantation hybridization stevia seed is obtained, the cured leaf per mu yield is 250～300 kilograms, and stevioside content reaches 12% of cured leaf gross weight in the cured leaf, and the per mu yield stevioside is 30～36 kilograms, and output improves greatly; Hybridization stevia kind is produced in the stevioside, and stevia rebaudianum disaccharide glucoside A content accounts for 45% of stevioside gross weight, and the stevioside quality is significantly improved; Because hybridization stevia kind obtains by seed seedling-raising, cultivates cost for every mu and only is 60 yuan, with respect to cuttage stevia kind, has significantly reduced seedling cost; Because hybridization stevia kind is easy to promote by seed seedling-raising.
In order to make cuttage stevia kind identical with sexual stevia kind blooming stage, make the fertilization of cuttage stevia kind fully, when growing seedlings, partition cuttage stevia perennial root cuttage stevia seedling that grows 4～6 pairs of true leaves that obtains and the sexual stevia seedling that grows 4～6 pairs of true leaves are mixed plantation simultaneously; Cuttage stevia kind is grown seedlings by perennial root, and sexual stevia is by seed seedling-raising, and the seedling growing process of cuttage stevia kind and sexual stevia kind is a known technology; During hybridization, sexual stevia kind can be broadcasted sowing earlier and be grown seedlings, when treating that seedling grows 4～6 pairs of true leaves, transplant land for growing field crops hybridization simultaneously with the cuttage stevia seedling that grows 4～6 pairs of true leaves that partition cuttage stevia perennial root obtains, to save sexual stevia seed consumption, also can be directly with sexual stevia seed broadcasting in the land for growing field crops, when treating that seedling grows 4～6 pairs of true leaves, the long cuttage stevia seedling that 4～6 pairs of true leaves are arranged is moved into the land for growing field crops mixes plantation; For the male pollen that guarantees sexual stevia kind provides abundant, so that cuttage stevia kind fully is fertilized, improve cuttage stevia kind knot kind of rate, plantation amount when as far as possible reducing sexual inter breed crossing simultaneously, improve cuttage stevia kind and produce the output of hybridization stevia seed, the strain ratio of counting of sexual stevia kind and cuttage stevia inter breed crossing plantation is 1: 3～5; In order to make the hybridization stevia enough growing space growths be arranged fully, improve the output of hybridization stevia seed simultaneously, during hybridization, every mu of total strain number of plantation of sexual stevia and cuttage stevia is 8000～10000 strains; Convenient and easy when making hybridization stevia seed results, cuttage stevia kind is planted with the interlacing of sexual stevia kind or every row.
The ratio of strain number by selecting sexual stevia kind and the inter breed crossing of cuttage stevia plantation is 1: 3～5, every mu of total strain number of plantation of the stevia of control hybridization simultaneously is 8000～10000 strains, after knot is planted, the recovery rate of hybridization stevia seed is 9～10 kilograms of per mu yields, and during plantation stevia Hybrid, grow seedlings for every mu and only need stevia hybrid seed 150 grams, and only need seeding and seedling raising, cultivate cost and significantly reduce.
Embodiment
The invention will be further described below in conjunction with embodiment:
Embodiment 1:
Broadcast sowing sexual stevia seed in one mu of soil, when treating that sexual stevia seedling grows 4～6 pairs of true leaves, remove seedling, keeping sexual stevia strain number is 500 strains, cuttage stevia seedling 6500 strains that will grow 4～6 pairs of true leaves simultaneously through growing seedlings move into and mix plantation, sexual stevia and the cuttage stevia crossbreed plant ratio of counting are 1: 13, sexual stevia and several 7000 strains of the cuttage stevia hybridization total strain of plantation, mark cuttage stevia kind planting location, after knot is planted, collect cuttage stevia variety seeds, the hybrid seed per mu yield is about 6 kilograms.
Embodiment 2:
Earlier sexual stevia and cuttage stevia are grown seedlings, in one mu of soil, transplant cuttage stevia seedling 7500 strains that grow sexual stevia seedling 1500 strains of 4～6 pairs of true leaves and grow 4～6 pairs of true leaves simultaneously, sexual stevia and the cuttage stevia crossbreed plant ratio of counting are 1: 5, sexual stevia and several 9000 strains of the cuttage stevia hybridization total strain of plantation, after knot is planted, different according to the cuttage stevia with sexual stevia outward appearance, collect cuttage stevia variety seeds, the hybrid seed per mu yield is about 10 kilograms.
Embodiment 3:
Earlier sexual stevia and cuttage stevia are grown seedlings, in one mu of soil, transplant simultaneously and grow 4～67500 strains, and with sexual stevia seedling and cuttage stevia seedling interlacing plantation, sexual stevia and the cuttage stevia crossbreed plant ratio of counting are 1: 3, sexual stevia and several 10000 strains of the cuttage stevia hybridization total strain of plantation, knot is collected cuttage stevia kind seed that row is tied separately after planting, and the hybrid seed per mu yield is about 9 kilograms.
Among the present invention, after cuttage stevia kind and sexual stevia kind are grown seedlings, get final product normal growth under normal natural conditions, can carry out the pollen transmission under common natural conditions, knot obtains hybridizing the stevia seed after planting.
Claims (5)
1, a kind of breeding method of stevia hybrid seed is characterized in that, is male parent with the sexual kind of stevia, is female parent with stevia cuttage kind, mixes plantation and hybridizes, and collects stevia cuttage seed that kind is produced.
2, according to the breeding method of the described stevia hybrid seed of claim 1, it is characterized in that, after growing seedlings, partition cuttage stevia perennial root seedling that grows 4～6 pairs of true leaves that obtains and the sexual stevia seedling that grows 4～6 pairs of true leaves are mixed plantation simultaneously hybridize.
According to the breeding method of the described stevia hybrid seed of claim 1, it is characterized in that 3, the crossbreed plant ratio of counting of sexual stevia kind and cuttage stevia kind is 1: 3～5.
According to the breeding method of claim 1,2 or 3 described stevia hybrid seeds, it is characterized in that 4, it is 8000～10000 strains that total amount is counted in the strain of every mu of stevia hybridization plantation.
5, the breeding method of stevia hybrid seed according to claim 4 is characterized in that, cuttage stevia kind and the interlacing of sexual stevia kind or every row plantations hybridization.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115131 CN1129354C (en) | 2001-07-11 | 2001-07-11 | Method for breeding hybridized seed of stevia rebaudinum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115131 CN1129354C (en) | 2001-07-11 | 2001-07-11 | Method for breeding hybridized seed of stevia rebaudinum |
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| Publication Number | Publication Date |
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| CN1327720A true CN1327720A (en) | 2001-12-26 |
| CN1129354C CN1129354C (en) | 2003-12-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 01115131 Expired - Fee Related CN1129354C (en) | 2001-07-11 | 2001-07-11 | Method for breeding hybridized seed of stevia rebaudinum |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933454A (en) * | 2010-09-26 | 2011-01-05 | 凤阳县小岗村润农甜叶菊高科有限公司 | Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents |
| US7884265B2 (en) | 2005-03-04 | 2011-02-08 | Toyoshige Morita | High Rebaudioside-A plant |
| CN1985575B (en) * | 2006-09-27 | 2012-05-30 | 王其斌 | Method for systematically selecting and breeding asexual stevia parent for producing hybrid |
| CN102715074A (en) * | 2011-11-01 | 2012-10-10 | 江苏省中国科学院植物研究所 | Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds |
| CN103651150A (en) * | 2012-09-11 | 2014-03-26 | 尚春生 | Novel-variety vegetable chrysanthemum feast with high protein and no vermin |
| CN106538201A (en) * | 2016-10-27 | 2017-03-29 | 浠水县冬容蔬菜专业合作社 | A kind of implantation methods of Herba chenopodii Artemisia |
-
2001
- 2001-07-11 CN CN 01115131 patent/CN1129354C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7884265B2 (en) | 2005-03-04 | 2011-02-08 | Toyoshige Morita | High Rebaudioside-A plant |
| CN1985575B (en) * | 2006-09-27 | 2012-05-30 | 王其斌 | Method for systematically selecting and breeding asexual stevia parent for producing hybrid |
| CN101933454A (en) * | 2010-09-26 | 2011-01-05 | 凤阳县小岗村润农甜叶菊高科有限公司 | Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents |
| CN102715074A (en) * | 2011-11-01 | 2012-10-10 | 江苏省中国科学院植物研究所 | Method for production of superior Stevia rebaudiana Bertoni. F1 generation seeds |
| CN103651150A (en) * | 2012-09-11 | 2014-03-26 | 尚春生 | Novel-variety vegetable chrysanthemum feast with high protein and no vermin |
| CN106538201A (en) * | 2016-10-27 | 2017-03-29 | 浠水县冬容蔬菜专业合作社 | A kind of implantation methods of Herba chenopodii Artemisia |
| CN106538201B (en) * | 2016-10-27 | 2020-01-07 | 浠水县冬容蔬菜专业合作社 | Planting method of artemisia selengensis |
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| Publication number | Publication date |
|---|---|
| CN1129354C (en) | 2003-12-03 |
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Assignee: Qingdao Runde Biotechnology Co., Ltd. Assignor: Chuangsheng Biologic Science and Technology Co., Ltd., Qingdao Contract record no.: 2010370000456 Denomination of invention: Method for breeding hybridized seed of stevia rebaudinum Granted publication date: 20031203 License type: Exclusive License Open date: 20011226 Record date: 20100816 |