CN101933454A - Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents - Google Patents
Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents Download PDFInfo
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- CN101933454A CN101933454A CN 201010291886 CN201010291886A CN101933454A CN 101933454 A CN101933454 A CN 101933454A CN 201010291886 CN201010291886 CN 201010291886 CN 201010291886 A CN201010291886 A CN 201010291886A CN 101933454 A CN101933454 A CN 101933454A
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Abstract
The invention discloses a method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents, which comprises the following steps of: selecting varieties at an asexual stage, preparing seeds of generation F1 by cross breeding at a sexual stage, and preparing seeds of generation F2 by using a backcross method. The method of the invention has the advantages of high leaf yield, high total glycoside content, high Rebaudioside-A (RA) glycoside content, high resistance and stable properties.
Description
Technical field
The present invention relates to a kind of breeding method of stevia improved seeds, specifically is the method that a kind of systematic breeding stevia father and mother originally cultivate good stevia seed.
Background technology
The sweetener that uses in the world has kind more than 20 at present, can be divided into natural and synthetic two big classes by its source.Natural class has sucrose, fructose, honey etc., and they all contain high heat, causes blood sugar for human body to raise and carious tooth diabetes patient, cardiovascular patient, adiposis patient inedibility easily.The chemosynthesis class has Aspartame, sweetener, asccharin etc., and this series products is harmful unhelpful.Stevioside is as the pure natural sweetener, its sugariness is 200-400 a times of sucrose, heat only is 1/300 of a sucrose, pure taste, edible safety, is well received in industries such as food, beverage, medicine, cosmetics in many countries use, being described as " natural asccharin ", the world " trisaccharide source ", is the best substitute of sucrose and other synthetic sweeteners.Suitable especially diabetes, cardiovascular disease, adiposis, high blood pressure and children caries patient are edible.Stevioside is healthy human natural sugar source, and prospect is very wide light, as the raw material of producing stevioside---and the prospect of production of stevia rebaudian leaf also is very wide light.
The kind method for planting that China uses existing stevia planting base has two kinds: the one, and asexual process, the advantage of this method is the purity and the fine quality that can keep kind; Shortcoming be Qiu Fan survive the winter female seedling, keep survive the winter kind of root and spring during numerous cultivation seedling, time-consuming, take a lot of work, take money, the production cost height, though existing cultivar RA salidroside content is higher, leaf-making quantity is low, variet complexity is serious.The 2nd, the sexual propagation method, compare with asexual process save time, saving of labor, province's money, production cost is low.But the shortcoming of this propagation method is a kind easily degenerates.When sexual propagation began to introduce a fine variety, Flos Chrysanthemi total glycosides content was more than 10%, and now total salidroside content has only about 6%.So existing two kinds of propagation methods are all undesirable.The Chinese patent patent document that publication number is CN1327720A on December calendar year 2001 26 discloses a kind of breeding method of stevia rebaudianum hybrid seed, is male parent with sexual kind to the effect that, and asexual cuttage kind is that female parent is hybridized, and collects maternal seed.But this method is owing to sexual male parent quality instability, and asexual female parent so the hybrid seed that makes is undesirable, can not satisfy the requirement of plantation family and processing enterprise not through preferred, can not satisfy the requirement of industries such as food, beverage, medicine, cosmetic.The Chinese patent CN1985575A that open day was on June 27th, 2007 discloses a kind of method of this cultivations of systematic breeding stevia rebaudianum clone father and mother hybrid new breed, but cultivates seed only through a colony hybridization by this method, the mixing F that gathers
1For the gained crossbreed, but this method still exists the unsettled shortcoming of proterties.So, cultivate the good new varieties of stevia, be the technical problem that current stevia plantation and processing press for solution, also be in order to solve healthy sugared source, to satisfy the key technical problem that human health requires.
Summary of the invention
The objective of the invention is to overcome the deficiency of existing stevia kind, cultivate a kind of leaf-making quantity height, total salidroside content height, RA salidroside content height, resistance (promptly three high resist), stevia improved seeds that proterties is stable.
To achieve these goals, the present invention is according to the rationale of seed selection stevia improved seeds: 1, Ye Ju have can sexual propagation again can vegetative characteristic theory.2, with the fixing theory of merit of vegetative propagation.3, heterotic theory.
The present invention has adopted following technology path seed selection stevia improved seeds:
1, the asexual stage kind is selected
2, perfect stage hybrid seeding
3, strengthen hybridizing F with the method for backcrossing
1Objective trait in generation
Particular content of the present invention:
1, the asexual stage kind is selected
Stevia is self-sterile cross pollinated plant, thereby the genomic constitution that causes stevia colony is heterozygous all the time, the inheritance of sexual progeny is difficult for stable, and favorable variation and harmful variation are retained simultaneously, selects fine individual plant to bring challenge and possibility to us.We are from external, domestic each different growing area, at the different vegetative stage of stevia, feature, characteristic, vegetative period, florescence, resistance and adaptability etc. according to individual plant are observed and are monitored, and the fine individual plant of selecting leaf-making quantity height, leaf to contain glucoside amount height, resistance isolates management.
2, perfect stage hybrid seeding
1. from then and the fine individual plant of choosing over the years, during the apolegamy combination, choose from the pedigree of source far away as far as possible that riotous growth, leaf-making quantity height, resistance, florescence are close, knot rate height, general glycoside and two high fine individual plants of RA salidroside content carry out combined hybrid and test.
2. 1 year then by the combination plant division planting seed of gathering in the kind garden, carry out the field observation record, carry out leaf-making quantity and leaf assay, therefrom select fine combination, as father, female parent.
3. according to the theory that can keep merit with vegetative propagation, the father of the fine combination of selecting, female parent are carried out vegetative propagation, keep its merit, and form the single-strain clone of father, female parent.The fine combination father of apolegamy, maternal clone are pressed field planting in 1: 1, and carrying out two is colony hybridization, mixes the F that gathers
1For hybrid seed.
3, with mixing the F that gathers
1Do male parent for hybrid seed, former asexual female parent is done female parent and is backcrossed, and has cultivated new Hybrid.During hybrid seeding father and mother this by 1: 4 ratio field planting, the seed of gathering obtains to hybridize the target new varieties.
The present invention compares existing other kind and has the following advantages:
1, hybridization F
2The feature of stevia: limb is upright, resistant to lodging, riotous growth, blade are roomy.
2, hybridization F
2Stevia new varieties best in quality: cured leaf per mu yield height, average 400-450 jin/mu is than other kinds raisings 20%; Total salidroside content height can reach 12.76%, improves 3-5% than other kinds; RA salidroside content height can reach 8.10%, improves more than 50% than sexual cultivar; Resistance, more disease-resistant than other kinds, the worm ability is strong.
In a word, systematic breeding stevia rebaudianum clone father and mother originally cultivate the method for good new varieties: be according to stevia have can sexual propagation again can vegetative characteristic theory, with strong theory, the heterotic theory of the fixing good property of vegetative propagation, around three high one anti-breeding objectives, adhere to constantly going on, the stevia improved seeds are constantly weeded out the old and bring forth the new, quality improves constantly, but also can cultivate the stevia new varieties according to the new combination of market demand apolegamy.Utilize the stevia hybrid new breed to cultivate, cost is low, profitable, with inevitable all kinds that partly or entirely replace existing sexual cultivation and asexual cultivation, creates abundant economic benefit and social benefit for plantation family, processing enterprise, country.
Embodiment
Description below by process of the present invention illustrates the specific embodiment of the invention.
1, the asexual stage kind is selected
Stevia is self-sterile cross pollinated plant, thereby the genomic constitution that causes stevia colony is heterozygous all the time, the inheritance of sexual progeny is difficult for stable, and favorable variation and harmful variation are retained simultaneously, selects fine individual plant to bring challenge and possibility to us.We are from external, domestic each different growing area, at the different vegetative stage of stevia, feature, characteristic, vegetative period, florescence, resistance and adaptability etc. according to individual plant are observed and are monitored, the fine individual plant 4 (numbering is respectively 01,02,03,04) of selecting leaf-making quantity height, leaf to contain glucoside amount height, resistance isolates management, in order to ensure the reliability of selecting fine individual plant, all to carry out 3 times to each fine individual plant and detect the mean value that fetches data (as follows).
Fine individual plant and existing sexual and vegetative propagation kind leaf contain glucoside amount and page or leaf output comparison sheet:
2, perfect stage hybrid seeding
1. the planting seed of fine individual plant 01,02,03,04 plant division being gathered in 1 year carries out the field observation record, and carries out leaf-making quantity and leaf assay in the kind garden.
2. according to the theory that can keep merit with vegetative propagation, the male parent 01 of the fine combination in the fine individual plant 01,02,03,04, maternal 02 is carried out vegetative propagation, keep its merit, and form the single-strain clone of father, female parent.By field planting in 1: 1, carrying out two was colony hybridization the clone of the fine combination male parent 01 of apolegamy, female parent 02, mixed the F that gathers
1For hybrid seed.
Male parent 01, female parent 02, hybridization F1, common variety leaf assay table:
As can be seen from the above table, hybridization F
1Content, the leaf-making quantity of the general glycoside in generation and RA glucoside all are greatly improved than common variety.
3, with mixing the F that gathers
1Do male parent for hybrid seed, former asexual maternal 02 does female parent backcrosses, and cultivates the Hybrid that makes new advances.During hybrid seeding father and mother this by 1: 4 ratio field planting, the seed of gathering obtains to hybridize the target new varieties.
Hybridization F
2General glycoside and the content of RA glucoside than hybridization F
1In generation, be greatly improved again.
Below by concrete application example of the present invention, further specify advantage of the present invention.
1, the Anhui bio tech ltd used 180 jin in stevia seed of the present invention in 2008,1000 mu of Anhui Province's plantations, average 8700 strains of every mu of total strain number of plantation, receive 256 tons of stevia rebaudianum cured leafs altogether, produce 32.67 tons of steviosides, average 7.84 tons of stevia rebaudianum cured leafs are produced 1 ton of stevioside, and sexual kind cured leaf is produced 1 ton of stevioside for 16.6 tons, cost reduces, and output increases, and income increases.The raising of stevioside content simultaneously also makes the sugariness multiple of stevioside improve, and then has reduced sweetener and added cost.
2,50 mu in this seed was planted in the Jiangsu Province village in 2008, average 8700 strains of every mu of total strain number of plantation, 450 jin of average yield per mu cured leafs, 2420 yuan of per mu yield values, 2125 yuan of net profit.Peasant income is higher than local other crop far away.
3,80 mu in seed of the present invention was bred in Suzhou City Yong Zhen township village in 2008, average 8700 strains of every mu of total strain number of plantation, 3150 yuan of stevia rebaudianum seed and cured leaf per mu yield values, 2685 yuan/mu of net profit.Peasant income is higher than when other economic crops such as tibet milkwort root, cottons far away.
Because the present effect of the present invention is good, stevia rebaudianum plantation family and production of hybrid seeds family economic well-being of workers and staff height; Stevioside processing enterprise high efficiency; Use the food quality of stevioside processing good; Stevia rebaudianum crossbreed of the present invention can be throughout the country popularizing planting rapidly, create abundant economic benefit and social benefit for plantation family, processing enterprise, country.
Claims (4)
1. method that systematic breeding stevia father and mother originally cultivate good stevia seed, its step comprises in order: the asexual stage kind is selected, perfect stage hybridization system F
1For seed, with the method system of backcrossing F
2For seed.
2. the method for originally cultivating good stevia seed according to the described systematic breeding stevia of claim 1 father and mother, when it is characterized in that in the described perfect stage hybrid seeding step apolegamy combination, choose from the pedigree of source far away that riotous growth, leaf-making quantity height, resistance, florescence are close, high two fine individual plants of knot rate carry out combined hybrid.
3. the method for originally cultivating good stevia seed according to the described systematic breeding stevia of claim 1 father and mother is characterized in that
Described perfect stage hybridization system F
1In generation, planted the fine combination father that matches in the substep, maternal clone by field planting in 1: 1.
4. the method for originally cultivating good stevia seed according to the described systematic breeding stevia of claim 1 father and mother is characterized in that
The described usefulness method system F that backcrosses
2For the ratio field planting of father and mother's basis in the kind substep in 1: 4.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012088612A1 (en) * | 2010-12-31 | 2012-07-05 | Glg Life Tech Corporation | High rebaudioside-a plant and methods of producing the same and uses thereof |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN113229136A (en) * | 2021-05-25 | 2021-08-10 | 南京农业大学 | Stevia rebaudiana leaf quality breeding parent matching method |
| CN113557953A (en) * | 2021-06-17 | 2021-10-29 | 宿州天一甜叶菊高科有限公司 | Method for cultivating new variety of stevia rebaudiana |
| CN113677196A (en) * | 2019-04-11 | 2021-11-19 | 三得利控股株式会社 | Stevia plant with low flower bud forming ability |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327720A (en) * | 2001-07-11 | 2001-12-26 | 青岛创升生物科技有限公司 | Method for breeding hybridized seed of stevia rebaudinum |
| CN1547893A (en) * | 2003-05-18 | 2004-11-24 | 杨有钊 | Improved single plant breeding process for stevia |
| CN1985575A (en) * | 2006-09-27 | 2007-06-27 | 王其斌 | Systemic asexual stevia parent selection breeding process for producing hybrid |
-
2010
- 2010-09-26 CN CN 201010291886 patent/CN101933454A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327720A (en) * | 2001-07-11 | 2001-12-26 | 青岛创升生物科技有限公司 | Method for breeding hybridized seed of stevia rebaudinum |
| CN1547893A (en) * | 2003-05-18 | 2004-11-24 | 杨有钊 | Improved single plant breeding process for stevia |
| CN1985575A (en) * | 2006-09-27 | 2007-06-27 | 王其斌 | Systemic asexual stevia parent selection breeding process for producing hybrid |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012088612A1 (en) * | 2010-12-31 | 2012-07-05 | Glg Life Tech Corporation | High rebaudioside-a plant and methods of producing the same and uses thereof |
| CN105052503A (en) * | 2015-08-23 | 2015-11-18 | 林平 | Hybrid seed production method for stevia rebaudiana |
| CN113677196A (en) * | 2019-04-11 | 2021-11-19 | 三得利控股株式会社 | Stevia plant with low flower bud forming ability |
| CN113229136A (en) * | 2021-05-25 | 2021-08-10 | 南京农业大学 | Stevia rebaudiana leaf quality breeding parent matching method |
| CN113229136B (en) * | 2021-05-25 | 2022-02-11 | 南京农业大学 | Stevia rebaudiana leaf quality breeding parent matching method |
| CN113557953A (en) * | 2021-06-17 | 2021-10-29 | 宿州天一甜叶菊高科有限公司 | Method for cultivating new variety of stevia rebaudiana |
| CN114946650A (en) * | 2021-06-17 | 2022-08-30 | 宿州天一甜叶菊高科有限公司 | Method for cultivating new variety of stevia rebaudiana |
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Application publication date: 20110105 |