CN106962172B - A kind of breeding method of Cowpea Variety kind - Google Patents
A kind of breeding method of Cowpea Variety kind Download PDFInfo
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- CN106962172B CN106962172B CN201710078847.5A CN201710078847A CN106962172B CN 106962172 B CN106962172 B CN 106962172B CN 201710078847 A CN201710078847 A CN 201710078847A CN 106962172 B CN106962172 B CN 106962172B
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- low temperature
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention discloses a kind of breeding method of Cowpea Variety kind, the following steps are included: (1) screens the Cowpea Germplasm Resources and material of precocious, low temperature resistant high yield from the local varieties and germ plasm resource of cowpea, it is selfed and is purified according to breeding objective, preparing hybrid combination again carries out conventional breeding, is selfed separating-purifying；(2) to the kind or strain seeding and seedling raising in 6 generations of selfing, the identification of seedling stage tolerance to low temperature and weak light is carried out；(3) the 6th day investigation germinating energy of nursery, nursery investigation emergence in the 12nd day, height of seedling, stem thickness, cotyledon are long × wide, calculate tolerance to low temperature and weak light sex index according to result is investigated；(4) by statisticalling analyze to qualification result, the kind or strain of the higher tolerance to low temperature and weak light of the index filtered out obtain the Cowpea Variety kind of tolerance to low temperature and weak light into variety test.The positive correlation with height is reacted to environmental condition with strain using seedling, it is screened by early stage seedling, identification of species or the tolerance to low temperature and weak light of strain in seedling stage, eliminate the kind or strain of tolerance to low temperature and weak light difference, effectively reduce the kind of screening or the quantity of strain, the workload for substantially alleviating field trial, accelerates breeding process.
Description
Technical field
The present invention is a kind of breeding method of cowpea variety, belongs to field of plant variety breeding technology, dedicated for breeding precocity
Cowpea variety.
Background technique
Traditional Cowpea Variety breeding for quality method is cell and repetition to be arranged, with check variety ratio using field trial
Compared with characters such as tolerance to low temperature and weak light, flower spike section and early yields (yield that control starts harvesting 15 days), to screen early cooked food
Kind, process is complicated, can consume more man power and material.Currently, the correlation of domestic Cowpea Variety breeding for quality not yet is specially
Benefit.
Summary of the invention
The purpose of the present invention is to provide a kind of breeding methods of Cowpea Variety kind.The present invention is to cowpea conventional breeding side
Method is improved.Tolerance to low temperature and weak light screening is carried out to cowpea variety or strain first, using seedling and strain to environmental condition
The positive correlation with height is reacted, is screened by early stage seedling, in Seedling Stage identification of species or the tolerance to low temperature and weak light of strain,
The kind or strain for eliminating tolerance to low temperature and weak light difference, effectively reduce the kind of screening or the quantity of strain, substantially alleviate
The workload of field trial.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of breeding method of Cowpea Variety kind, comprising the following steps:
(1) Cowpea Germplasm Resources and material of precocious, low temperature resistant high yield are screened from the local varieties of cowpea and germ plasm resource
Material, according to breeding objective be selfed purification selection 1~2 generation, according to separate sources be selfed based material performance, preparing hybrid combine into
Row conventional breeding is selfed separating-purifying, is selfed for 6 generations；
(2) to the kind or strain in 6 generations of selfing, carry out the identification of seedling stage tolerance to low temperature and weak light: by multiple kinds to be identified or
Strain selects the seed for meeting kind or strain characteristic, rejects granule seed, lopsided seed and big grain seed, is educated using seedling medium sowing
Seedling；
(3) the 6th day investigation germinating energy of nursery, nursery investigation emergence in the 12nd day, height of seedling, stem thickness, cotyledon are long × wide, according to examining
It examines result and calculates tolerance to low temperature and weak light sex index, qualification result statistical analysis technique is as follows:
(4) by statisticalling analyze to qualification result, the tolerance to low temperature and weak light sex index being calculated arranges from high to low, eliminates
The lower namely not kind or strain of tolerance to low temperature and weak light of index, the kind of the higher tolerance to low temperature and weak light of the index filtered out or
Strain obtains the Cowpea Variety kind of tolerance to low temperature and weak light into variety test.
Using the process of seedling medium seeding and seedling raising in step (2) are as follows: seed is seeded into seedlings nursing plate, is placed on artificial
Temperature setting is 20~22 DEG C of daytime by climate box, 8~12 DEG C at night；5000lux on daytime, evening 0lux are set by illumination；
Day time is 8:00~19:00, and be 19:00~8:00 the time in the evening, simulates the nursery of low temperature and poor light environmental condition.
Beneficial effects of the present invention:
The present invention improves cowpea conventional breeding methods.Being reacted with strain environmental condition using seedling has height
Positive correlation, screened by early stage seedling, in seedling stage identification of species or the tolerance to low temperature and weak light of strain, eliminate tolerance to low temperature and weak light
Property difference kind or strain, effectively reduce the kind of screening or the quantity of strain, substantially alleviate the work of field trial
Amount, accelerates breeding process.
Specific embodiment
Embodiment 1
(1) cowpea local varieties and germ plasm resource are collected and arranged, the various germ plasm resources of more than 46 parts is shared and material (is shown in Table
1).On the basis of collection and arrangement, field planting observation is carried out.Therefrom emphasis screens the cowpea kind of precocious, low temperature resistant high yield
Matter resource and material are selfed purification 1~2 generation of selection according to breeding objective.It is selfed the performance of based material according to separate sources, prepares
Cross combination 12, conventional breeding is carried out, is selfed separating-purifying, was selfed for 6 generations.
1 Cowpea Germplasm Resources of table
(2) to 37 strain offsprings in 6 generations of selfing, the identification of seedling stage tolerance to low temperature and weak light is carried out.Strain to be determined is selected
The seed for meeting this strain characteristic is rejected small seed, lopsided seed and big grain seed and is sowed seed using seedling medium seeding and seedling raising
Into seedlings nursing plate, every strain sows 200, sows spacing 4cm square, is placed in growth cabinet.By artificial climate box temperature
Degree is set as 20~22 DEG C of daytime, and 8~12 DEG C at night；5000lux on daytime, evening 0lux are set by illumination；Day time is
8:00~19:00, are 19:00~8:00 the time in the evening, simulate the nursery of low temperature and poor light environmental condition.The other management of cowpea seedling are normal
It carries out.
(3) the 6th day investigation germinating energy of nursery, nursery investigation emergence in the 12nd day, height of seedling, stem thickness, cotyledon are long × wide, according to examining
It examines result and calculates tolerance to low temperature and weak light sex index.Qualification result statistical analysis technique is as follows:
Tolerance to low temperature and weak light sex index=germinating energy (index)+emergence (index)-height of seedling (index)+stem thickness (index)+cotyledon
Long × wide (index)
(4) by statisticalling analyze to qualification result, the tolerance to low temperature and weak light sex index being calculated arranges from high to low, eliminates
The lower namely not strain of tolerance to low temperature and weak light 29 of index, the strain of the higher tolerance to low temperature and weak light of the index filtered out 8,
Into variety test.Wherein 05-8-4-2-1-6-3 strain behaves oneself best, and tolerance to low temperature and weak light sex index is 3.4.
(5) 2008 years~2009 8 strains to the tolerance to low temperature and weak light filtered out carry out variety comparative test and pilot production.
An elite plant strain 05-8-4-2-1-6-3 is filtered out, early yield is close with " the peaceful vegetable spring is early ", and total output is more than " the peaceful vegetable spring
It is early ".General early yield is up to 800kg/667m2, total output is up to 2000kg/667m2More than, than " cowpea 28-2 " volume increase
30% or more, early yield accounts for about the 40% of total output.Formally name for the peaceful vegetable spring it is rich.Variety comparative test and mill trial results are just
It is good consistent with Seedling Stage low temperature and poor light screening conclusion height.
Rich feature of peaceful vegetable spring: plant overgrows, and growth potential is strong, born pods with main stem based on, main stem 2-3, which is saved, gives birth to the
1 flower spike, flower are light blue purple, and maturity is early, and single plant bears pods more, early yield height.Long 70cm of pod or so, 0.8~1.0cm of transverse diameter,
30~35g of single pod quality.Pod puts on flesh thickness, and matter is tender and crisp, and fiber is few, sweet；The green white of pod, seed bronzing.Later period is not easy early ageing.
Compared with anti-blight and sooty mould, have prematureness good, high yield, it is best in quality the features such as.It is suitable in the Yangtze river basin and all parts of the country
Early spring protecting field and spring outdoor cropping.
Claims (2)
1. a kind of breeding method of Cowpea Variety kind, it is characterised in that the following steps are included:
(1) Cowpea Germplasm Resources and material of precocious, low temperature resistant high yield are screened from the local varieties of cowpea and germ plasm resource,
It is selfed purification 1 ~ 2 generation of selection according to breeding objective, the performance of based material is selfed according to separate sources, preparing hybrid combination carries out normal
Breeding is advised, separating-purifying is selfed, was selfed for 6 generations；
(2) to the kind or strain in 6 generations of selfing, the identification of seedling stage tolerance to low temperature and weak light is carried out: by multiple kinds to be identified or strain
The seed for meeting kind or strain characteristic is selected, granule seed, lopsided seed and big grain seed are rejected, using seedling medium seeding and seedling raising；
(3) the 6th day investigation germinating energy of nursery, nursery investigation emergence in the 12nd day, height of seedling, stem thickness, cotyledon are long × wide, tie according to investigating
Fruit calculates tolerance to low temperature and weak light sex index, and qualification result statistical analysis technique is as follows:
Number of emerging/sowing number of Germination energy index=after planting the 6th day
It emerges number of emerging/sowing number of index=after planting the 12nd day
Height of seedling index=this kind average height of seedling/all tested varieties or strain average height of seedling
Stem thickness that stem thickness index=this kind is averaged stem thickness/all tested varieties or strain is averaged
The product of length and width that long × wide index=this kind of cotyledon is averaged product/all tested varieties of length and width or strain is averaged
Long × wide index of tolerance to low temperature and weak light sex index=Germination energy index+emergence index-height of seedling index+stem thickness index+cotyledon
(4) by statisticalling analyze to qualification result, the tolerance to low temperature and weak light sex index being calculated arranges from high to low, eliminates index
The lower namely not kind or strain of tolerance to low temperature and weak light, the kind or strain of the higher tolerance to low temperature and weak light of the index filtered out
System obtains the Cowpea Variety kind of tolerance to low temperature and weak light into variety test.
2. the breeding method of Cowpea Variety kind according to claim 1, it is characterised in that use nursery base in step (2)
The process of matter seeding and seedling raising are as follows: seed is seeded into seedlings nursing plate, growth cabinet is placed on, by temperature setting be daytime 20 ~
22 DEG C, at night 8 ~ 12 DEG C；5000lux on daytime is set by illumination, at night 0 lux；Day time is 8:00 ~ 19:00, at night
Time is 19:00 ~ 8:00, simulates the nursery of low temperature and poor light environmental condition.
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| CN201710078847.5A CN106962172B (en) | 2017-02-14 | 2017-02-14 | A kind of breeding method of Cowpea Variety kind |
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| CN106962172A CN106962172A (en) | 2017-07-21 |
| CN106962172B true CN106962172B (en) | 2019-05-03 |
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| CN112715348A (en) * | 2020-12-29 | 2021-04-30 | 山西省农业科学院小麦研究所 | Breeding method of high-resistance soybean |
| CN114766302B (en) * | 2022-03-16 | 2024-02-20 | 江西农业大学 | Screening method of direct seeding early rice material with strong rebound growth after low temperature stress |
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| CN103597968A (en) * | 2013-08-27 | 2014-02-26 | 南通市通州区农林科学研究所 | Greenhouse efficient cultivation mode for early spring tomatoes, summer cowpeas and autumn Chinese parsleys |
| CN104255445A (en) * | 2014-09-15 | 2015-01-07 | 上海市农业科学院 | Selective breeding method of new spring-sowing ecological type vegetable soybean variety |
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