CN114946650A - Method for cultivating new variety of stevia rebaudiana - Google Patents
Method for cultivating new variety of stevia rebaudiana Download PDFInfo
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- 244000228451 Stevia rebaudiana Species 0.000 title claims abstract description 21
- 235000006092 Stevia rebaudiana Nutrition 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000009395 breeding Methods 0.000 claims abstract description 76
- 241000196324 Embryophyta Species 0.000 claims abstract description 58
- 230000001488 breeding effect Effects 0.000 claims abstract description 46
- 238000009396 hybridization Methods 0.000 claims abstract description 21
- WGLYADVYCJUFAY-UHFFFAOYSA-N cloran Chemical compound C12CC3C(=O)OC(=O)C3CC2C2(Cl)C(Cl)=C(Cl)C1(Cl)C2(Cl)Cl WGLYADVYCJUFAY-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241000544066 Stevia Species 0.000 claims abstract description 18
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 claims abstract description 17
- 230000008901 benefit Effects 0.000 claims abstract description 13
- 235000009508 confectionery Nutrition 0.000 claims abstract description 9
- 238000012360 testing method Methods 0.000 claims description 51
- 238000012364 cultivation method Methods 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 4
- 238000002955 isolation Methods 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 239000010902 straw Substances 0.000 claims description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 4
- 240000007594 Oryza sativa Species 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 229930182478 glucoside Natural products 0.000 abstract description 4
- 150000008131 glucosides Chemical class 0.000 abstract description 4
- 238000010899 nucleation Methods 0.000 abstract description 3
- JRBPAEWTRLWTQC-UHFFFAOYSA-N dodecylamine Chemical compound CCCCCCCCCCCCN JRBPAEWTRLWTQC-UHFFFAOYSA-N 0.000 abstract 1
- 241000209094 Oryza Species 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 239000011049 pearl Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000723353 Chrysanthemum Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- UGNWTBMOAKPKBL-UHFFFAOYSA-N tetrachloro-1,4-benzoquinone Chemical compound ClC1=C(Cl)C(=O)C(Cl)=C(Cl)C1=O UGNWTBMOAKPKBL-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for cultivating a new stevia variety, which belongs to the technical field of methods for cultivating new stevia varieties and is used for breeding excellent parents; selecting a self-bred excellent strain original field P multiplied by the fragrant and sweet No. 1 combination for hybridization and breeding a Huinong No. 3 strain as a parent 1; selecting the bred excellent variety Huinong No. 3 and Rundd No. 1 to carry out combined hybridization to breed a Huinong No. 9 variety as a parent 2; selecting a cultured excellent strain: dry breeding, group crossing of wild P, father P1, father P2 and Wangxincg group, and breeding WPL strain as parent 3. In the invention, a plurality of parents are clustered to select excellent single plants, so the breeding efficiency is high; the bred offspring has stable characters, good consistency and excellent economic characters; the bred new variety is suitable for direct seeding cultivation of seeds; the new pearl amine 12 has the advantages that: the content of glucoside is high, the yield of stevia rebaudiana leaves is high, the new variety of the chloran 12 stevia rebaudiana is strong in resistance, stable in character and not easy to degrade, the cultivation management is easy, the cost is low, and the benefit is good.
Description
Technical Field
The invention relates to a cultivation method of a new stevia variety, and belongs to the technical field of cultivation methods of new stevia varieties.
Background
The traditional breeding method of new stevia rebaudiana variety is systematic breeding and crossbreeding, and has the following disadvantages: the system breeding method has long time and complex process, and the breeding process needs to occupy a large amount of the existing seed resources; selecting particularly good and stable varieties is difficult; the disadvantages of the crossbreeding method are: the separation phenomenon of the offspring is serious, and the selection of ideal offspring with stable characters is extremely difficult; meanwhile, the conventional stevia production has the defects of low yield of dry leaves of varieties, low glucoside content and low benefit of chrysanthemum farmers; the cultivation method is time-consuming, labor-consuming and high in production cost.
How to research a cultivation method of a new stevia rebaudiana variety is a problem to be solved urgently at present.
Disclosure of Invention
The invention mainly aims to solve the defects of the prior art and provides a method for cultivating a new stevia variety.
The purpose of the invention can be achieved by adopting the following technical scheme:
a cultivation method of a new stevia rebaudiana variety comprises the following steps:
step 1: breeding excellent parents; selecting a self-bred excellent strain original field P multiplied by the fragrant and sweet No. 1 combination for hybridization and breeding a Huinong No. 3 strain as a parent 1; selecting the bred excellent variety Huinong No. 3 and Rundd No. 1 to carry out combined hybridization to breed a Huinong No. 9 variety as a parent 2; selecting a cultured excellent strain: dry breeding, carrying out group crossing on the original P, the father P1, the father P2 and the Wangxincg group, and selectively breeding a WPL strain as a parent 3;
step 2: performing group crossing on excellent parents; planting the parent 1, the parent 2 and the parent 3 in a mixed manner, grouping and carrying out group crossing, and performing plant division and seed collection;
and step 3: and (3) carrying out seedling cultivation on the seeds harvested in the plant division manner, selecting an excellent single plant, carrying out field cultivation on the excellent single plant, observing the characters of the whole growth period, testing the yield and the content of dry leaves, and carrying out comprehensive analysis to select the best single plant, namely chloran 12.
Further, breeding the parent 1: from main stevia rebaudiana cultivation areas and different growth stages of stevia rebaudiana, the following 8 combinations are formed by selecting and determining excellent single plants according to different growth traits: b04-1 XB 05-4, B04-1 XB 05-6, B04-1 XB 05-plus, B04-1 XB 06-8, B04-1 XB 06-18, B05-6 XB 06-8, B06-8 XB 06-18, B05-6 XB 06-18.
Further, the field cultivation is managed under isolation conditions, and the seeds are harvested by grouping and grouping the parents and separating the plants.
Furthermore, when the seeds are subjected to seedling culture and cultivation tests, the characteristics, the flowering phase, the resistance and the comprehensive field performance of the seeds are observed and recorded; when the seeds are subjected to seedling culture and cultivation tests, the leaf yield and the leaf content are tested; the comprehensive analysis compares the selection with the advantages and the disadvantages, and the selection of the excellent selection primarily confirms that B04-1 XB 05-and-c and B05-6 XB 06-18 are excellent combinations.
Furthermore, B04-1 XB 05-c and B05-6 XB 06-18 are continuously subjected to seed production and cultivation tests, each index is stably represented by B05-6 XB 06-18, and finally the excellent combination is determined to be named as Huinong III and is named as parent 1.
Further, parent 2 breeding: taking 'Huinong No. 3' as a female parent and 'Rundd No. 1' of self-breeding as a male parent, carrying out hybridization, and harvesting seeds of the male parent and the female parent.
Furthermore, the harvested seeds are used for seedling raising, cultivation and test, and the comprehensive field performances such as characters, characteristics, resistance and the like are observed and recorded; testing the leaf yield and leaf content; through comprehensive analysis and comparison, a good single plant F08-6 is selected from seedlings grown from seeds harvested from the parent Rundd No. 1.
Further, taking Rundd No. 1 as a female parent and F08-6 as a male parent, hybridizing, harvesting seeds of the male and female parents, and carrying out seedling raising, cultivation and test on the harvested seeds; observing and recording the characters, characteristics, resistance and field comprehensive performance of the rice; the method comprises the steps of testing the leaf yield and the leaf content, comprehensively analyzing and comparing, selecting an excellent single plant F12-5 from seedlings grown from seeds harvested from a male parent F08-6, carrying out field cultivation test and field popularization planting on the F12-5, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing F12-5 for vigorous growth, stout straws and dark green leaves, and naming F12-5 as a Huinong 9, namely a parent 2, wherein various testing indexes are stable.
Further, breeding of the parent 3: with elite lines of self-breeding germplasm resources: sweet No. 1, Ming P: 19-4, LL: ra19-52, LL: ra19-17, LL: selfing with Ra 19-49;
with elite lines of self-breeding germplasm resources: the original field P: ra19-4 × Dry Breeding: HY;
LL: ra19-49 × Ming P: ra19-4 for combinatorial hybridization;
excellent lines with self-bred germplasm resources: performing group crossing on dry breeding, wild P, father P1, father P2 and WPL;
with elite lines of self-breeding germplasm resources: the original field P: ra19-4 × Dry Breeding: HY;
LL: ra19-49 × Ming P: ra19-4 for hybridization;
with elite lines of self-breeding germplasm resources: dry breeding, wild P, father P1, father P2, WPL group crossing;
the method comprises the steps of carrying out seed collection, seedling culture and cultivation on the plants, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing and comparing, and selecting a good single plant, namely a parent 3, named as chloran 9 from seedlings grown by seeds harvested from the mass-bred WPL.
Further, carrying out group crossing: carrying out group crossing on the group of the parent 1, the parent 2 and the parent 3, carrying out plant collection and seedling cultivation by dividing plants, observing and recording various characters of the whole growth period, and testing the yield and the content of dry leaves; through comprehensive analysis and comparison, excellent single plants are selected from seedlings raised by seeds harvested from parents 3, the excellent single plants are subjected to field cultivation experiments and field popularization planting, various characters in the whole growth period are tracked, observed and recorded, the yield and the content of dry leaves are tested, and the excellent single plants are named as chloran 12.
The invention has the beneficial technical effects that: according to the breeding method of the new stevia rebaudiana variety, the breeding method of the new stevia rebaudiana variety provided by the embodiment, parents and parents all adopt the excellent strains bred by the inventor to carry out selfing, hybridization and group crossing to breed the new stevia rebaudiana variety, and the breeding method has the advantages of high efficiency, stable character of bred offspring, excellent economic character, good consistency and suitability for direct seeding and cultivation of seeds.
Drawings
FIG. 1 is a drawing of a table comparing parent 1 with common varieties according to the present invention;
FIG. 2 is a drawing of a comparative table of parent 2 and parent 1 according to the present invention;
FIG. 3 is a drawing of a table comparing parent 3 to parent 2 according to the present invention;
FIG. 4 is a diagram of parent indices in accordance with the present invention;
FIG. 5 is a graph of the results of various indicators of chloranil 12 in accordance with the present invention;
FIG. 6 shows pearlamine 12 according to the invention in a table of common breeds.
Detailed Description
In order to make the technical solutions of the present invention more clear and definite for those skilled in the art, the present invention is further described in detail below with reference to the examples and the accompanying drawings, but the embodiments of the present invention are not limited thereto.
As shown in fig. 1 to fig. 6, the cultivation method of a new stevia species provided in this embodiment includes cultivating individual plant raw materials, as shown in the following table:
wherein the names of the above-mentioned lines are the material names in the following contents, and in the following biological materials, sweet No. 1 and Ming P: 19-4, LL: ra19-52, LL: ra19-17 and LL: ra19-49 is material of type sold in market, and all biological materials satisfy RA glycoside, TSG glycoside and RA/TSG ratio, and corresponding strain names can be compounded.
Also comprises the following steps:
step 1: breeding excellent parents; selecting a self-bred excellent strain original field P multiplied by the fragrant and sweet No. 1 combination to breed a Huinong No. 3 strain as a parent 1; selecting the bred excellent variety Huinong No. 3 and Rundd No. 1 to carry out combined hybridization to breed a Huinong No. 9 variety as a parent 2; selecting a cultured excellent strain: dry breeding, carrying out group crossing on the original P, the father P1, the father P2 and the Wangxincg group, and selectively breeding a WPL strain as a parent 3;
step 2: performing group crossing on excellent parents; planting the parent 1, the parent 2 and the parent 3 in a mixed manner, grouping and carrying out group crossing, and performing plant division and seed collection;
and step 3: and (3) carrying out seedling cultivation on the seeds harvested in the plant division manner, selecting an excellent single plant, carrying out field cultivation on the excellent single plant, observing the characters of the whole growth period, testing the yield and the content of dry leaves, and carrying out comprehensive analysis to select the best single plant, namely chloran 12.
And (3) parent 1 breeding: from the main stevia cultivation area and different growth stages of stevia in China, the following 8 combinations are formed by selecting and confirming excellent single plants according to different growth traits: b04-1 XB 05-4, B04-1 XB 05-6, B04-1 XB 05-and ③ B04-1 XB 06-8, B04-1 XB 06-18, B05-6 XB 06-8, B06-8 XB 06-18 and B05-6 XB 06-18.
And (4) managing the field cultivation under the isolation condition, and performing grouping and joint division on the parent and the female parent to harvest seeds.
When the seeds are subjected to seedling culture and cultivation tests, the characteristics, the flowering phase, the resistance and the comprehensive field performance of the seeds are observed and recorded; when the seeds are subjected to seedling culture and cultivation tests, the leaf yield and the leaf content are tested; the comprehensive analysis is carried out to compare and select the advantages and the disadvantages, and the selection of the advantages and the disadvantages preliminarily confirms that B04-1 XB 05-jia (a third generation of carbon) and B05-6 XB 06-18 are good combinations.
Continuously carrying out seed production and cultivation tests on B04-1 XB 05-c and B05-6 XB 06-18, wherein each index is stably represented by B05-6 XB 06-18, and finally determining that the combination is an excellent combination named as Huinongsan and is the parent 1.
And (3) parent 2 breeding: taking 'Huinong No. 3' as a female parent and 'Rundd No. 1' of self-breeding as a male parent, carrying out hybridization, and harvesting seeds of the male parent and the female parent.
The harvested seeds are used for seedling raising, cultivation and test, and the comprehensive field performances such as characters, characteristics, resistance and the like are observed and recorded; the leaf yield and leaf content were tested. Through comprehensive analysis and comparison, a good single plant F08-6 is selected from seedlings grown from seeds harvested from the parent Rundd No. 1.
Taking Rundd No. 1 as a female parent and F08-6 as a male parent, hybridizing, harvesting seeds of the male and female parents, and carrying out seedling raising, cultivation and test on the harvested seeds; observing and recording the characters, characteristics, resistance and field comprehensive performance of the rice; the method comprises the steps of testing the leaf yield and the leaf content, comprehensively analyzing and comparing, selecting an excellent single plant F12-5 from seedlings grown from seeds harvested from a male parent F08-6, carrying out field cultivation test and field popularization planting on the F12-5, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing F12-5 for vigorous growth, stout straws and dark green leaves, and naming F12-5 as a Huinong 9, namely a parent 2, wherein various testing indexes are stable.
And (3) breeding of the parent: with elite lines of self-breeding germplasm resources: sweet No. 1, Ming P: 19-4, LL: ra19-52, LL: ra19-17, LL: selfing with Ra 19-49;
with elite lines of self-breeding germplasm resources: (mother) wild P: ra19-4 × (father) dry breeding: HY;
(mother) LL: ra19-49 (father) Ming P: ra19-4 for combinatorial hybridization;
excellent lines with self-bred germplasm resources: performing group crossing on dry breeding, wild P, father P1, father P2 and WPL;
with elite lines of self-breeding germplasm resources: (mother) wild P: ra19-4 × (father) dry breeding: HY;
(mother) LL: ra19-49 (father) Ming P: ra19-4 for hybridization;
with elite lines of self-breeding germplasm resources: dry breeding, wild P, father P1, father P2, WPL group crossing;
the method comprises the steps of carrying out seed collection, seedling culture and cultivation on the plants, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing and comparing, and selecting a good single plant, namely a parent 3, named as chloran 9 from seedlings grown by seeds harvested from the mass-bred WPL.
Grouping and group crossing: carrying out group crossing on the group of the parent 1, the parent 2 and the parent 3, carrying out plant collection and seedling cultivation by dividing plants, observing and recording various characters of the whole growth period, and testing the yield and the content of dry leaves; through comprehensive analysis and comparison, excellent single plants are selected from seedlings raised by seeds harvested from parents 3, the excellent single plants are subjected to field cultivation experiments and field popularization planting, various characters in the whole growth period are tracked, observed and recorded, the yield and the content of dry leaves are tested, and the excellent single plants are named as chloran 12.
The breeding of the new pearl ran 12 stevia variety is superior from the varieties with the time interval of twenty years.
Firstly, breeding a parent 1:
1. from the main stevia cultivation area and different growth stages of stevia in China, the following 8 combinations are formed by selecting and confirming excellent single plants according to different growth traits: b04-1 XB 05-4, B04-1 XB 05-6, B04-1 XB 05-and ③ B04-1 XB 06-8, B04-1 XB 06-18, B05-6 XB 06-8, B06-8 XB 06-18 and B05-6 XB 06-18.
2. And (4) managing under the isolation condition, and performing grouping and grouping on the parent and the maternal plant to harvest seeds.
3. Carrying out seedling culture and cultivation tests on seeds of the hybrid rice, and observing and recording field comprehensive performances such as characteristics, flowering phase, resistance and the like; testing the leaf yield and leaf content; the comprehensive analysis is carried out to compare and select the advantages and the disadvantages, and the selection of the advantages and the disadvantages preliminarily confirms that B04-1 XB 05-jia (a third generation of carbon) and B05-6 XB 06-18 are good combinations. Continuously carrying out seed production and cultivation tests on B04-1 XB 05-c and B05-6 XB 06-18, wherein each index is stably represented by B05-6 XB 06-18, and finally determining that the combination is an excellent combination named as Huinongsan and is the parent 1.
As shown in fig. 1: parental 1 vs common varieties table:
as can be seen from table 1:
(1) the RA content is increased from 1.85 to 5.4, which is 192%.
(2) The TSG is improved from 7.66 to 10.03 by 52.68 percent
(3) The RA/TSG increased from 24.15 to 52.68 by 118%.
(4) The yield of dry leaves is improved from 211 KG/mu to 256 KG/mu, which is improved by 21.32 percent.
II, breeding the parent 2:
1. taking 'Huinong No. 3' as a female parent and 'Rundd No. 1' of self-breeding as a male parent, carrying out hybridization, and harvesting seeds of the male parent and the female parent.
2. And (5) carrying out seedling raising, cultivation and test on the harvested seeds. Observing and recording the comprehensive field performances such as characters, characteristics, resistance and the like; the leaf yield and leaf content were tested. Through comprehensive analysis and comparison, a good single plant F08-6 is selected from seedlings grown from seeds harvested from the parent Rundd No. 1.
3. Taking Rundd No. 1 as female parent and F08-6 as male parent, hybridizing, and collecting seeds of different parents. The harvested seeds are used for seedling raising, cultivation and test; observing and recording the comprehensive field performances such as characters, characteristics, resistance and the like; the leaf yield and leaf content were tested. And through comprehensive analysis and comparison, selecting an excellent single plant F12-5 from seedlings grown by seeds harvested from the male parent F08-6.
4. And F12-5 is subjected to field cultivation test and field popularization planting in the two-year period, various characters in the whole growth period are tracked, observed and recorded, and the yield and the content of dry leaves are tested. F12-5 was analyzed comprehensively for vigorous growth, strong straw, and green leaves. Each test index is relatively stable, and F12-5 is named as Huinong No. 9, namely parent 2.
As shown in fig. 2: table for comparison of parent 2 to parent 1:
as can be seen from table 2:
(1) the RA content is increased from 5.4 to 10.58, which is 95.92%.
(2) The TSG is improved from 10.3 to 15.12, which is improved by 46.79 percent
(3) The RA/TSG increased from 52.63 to 63.57, an increase of 10.89%.
(4) The yield of dry leaves is improved from 256 KG/mu to 300 KG/mu, which is improved by 17.18 percent.
Thirdly, breeding the parent 3:
1. with elite lines of self-breeding germplasm resources: sweet No. 1, Ming P: 19-4, LL: ra19-52, LL: ra19-17, LL: selfing with Ra 19-49;
2. with elite lines of self-breeding germplasm resources: (mother) wild P: ra19-4 × (father) dry breeding: HY;
(mother) LL: ra19-49 (father) Ming P: ra19-4 for combinatorial hybridization;
3. with elite lines of self-breeding germplasm resources: performing group crossing on dry breeding, wild P, father P1, father P2 and WPL;
the excellent strain of the self-breeding germplasm resources used in 2018: (mother) wild P: ra19-4 × (father) dry breeding: HY;
(mother) LL: ra19-49 (father) Ming P: ra19-4 for hybridization;
with elite lines of self-breeding germplasm resources: dry breeding, wild P, father P1, father P2, WPL group crossing;
and (4) carrying out seed collection, seedling raising and cultivation by dividing plants, tracking, observing and recording various characters in the whole growth period, and testing the yield and the content of dry leaves. Through comprehensive analysis and comparison, an excellent individual plant, named as chloranil 9, is selected from seedlings grown from seeds harvested from the mass-bred WPL, namely a parent 3.
As shown in fig. 3: table for comparison of parental 3 with parental 2:
as can be seen from table 3:
(1) the RA content is increased from 10.58 to 13.08, which is increased by 23.63 percent;
(2) the TSG is improved from 15.12 to 16.2 by 7.14 percent;
(3) RA/TSG is improved from 52.63 to 63.57, which is improved by 17.17%;
(4) the yield of dry leaves is improved from 256 KG/mu to 300 KG/mu, which is improved by 16.67%.
As shown in fig. 4: the index lists of parent 1, parent 2 and parent 3 are shown in the table.
Fourthly, group crossing:
group crossing is carried out on the group of the parent 1, the parent 2 and the parent 3, the plants are divided for seed collection and seedling cultivation, various characters of the whole growth period are observed and recorded, and the yield and the content of dry leaves are tested. Through comprehensive analysis and comparison, selecting excellent single plants from seedlings grown from seeds harvested from the parents 3, carrying out field cultivation tests, carrying out field popularization and planting, tracking, observing and recording various characters in the whole growth period, and testing the yield and the content of dry leaves. And (3) comprehensive analysis: chloran 12 is vigorous in growth, 2-3.5cm in leaf spacing, 7-10 branches and bluish green in leaf color, and the chloran 12 is named as chloran 12 after all indexes are tested stably.
As shown in fig. 5: the following results of the tests on the chloran 12 indexes are shown:
the chloran 12 is subjected to a field cultivation experiment for three consecutive years, combined with field popularization and planting, tracking, observing and recording all characters in the whole growth period, and testing the yield and the content of dry leaves. And (3) comprehensive analysis: chloran 12 is vigorous in growth, stable in character, 2-3.5cm in leaf spacing, 7-10 branches and green in leaf color. All the test indexes are ideal.
The seeds are directly sown by chloran 12 seeds for three years, and the growth period of the stevia rebaudiana is good. All indexes are greatly improved compared with common varieties.
As shown in fig. 6: chloran 12 is shown in comparison with the general breeds:
as can be seen from table 6:
(1) the RA content increased from 1.85 to 13.55, which is 632.4%.
(2) The TSG increased from 15.12 to 16.2 by 123.2%.
(3) RA/TSG increased from 52.63 to 63.57, an increase of 55.09%.
(4) The yield of dry leaves is improved from 211 KG/mu to 382 KG/mu, which is improved by 81 percent.
Chloran 12 seeds are directly sown for three years, and compared with cuttage seedlings, labor, time and cost are saved; the yield and the glucoside content of the dry leaves are greatly improved, and the economic benefit is improved; creates great economic and social benefits for chrysanthemum growers and stevia sugar processing enterprises and countries.
Example 1:
2.3 mu of stevia is planted, and 12 pearls are directly sown and cultivated; the average 385 kg/mu of dry leaves are harvested, and the content RA: 13.50 of; STV: 1.55: TSG: 17.2; RA/TSG: 78.4 percent. Because of the high glycoside content, the dry leaves are 11 yuan per kg. The normal variety has 6 yuan dry leaves per kilogram.
Example 2:
1 mu of stevia is planted, and chloran 12 is planted directly; the average harvested dry leaves is 358 kg/mu, and the content RA: 14.50 of the total weight of the powder; STV: 1.50: TSG: 17.6; RA/TSG: 82.4 percent. High glucoside content, and 12 yuan per kg of dry leaves. The normal variety has 6 yuan dry leaves per kilogram.
Example 3:
1.5 mu of stevia is planted, and 12 pearls are directly sown and cultivated; the average 385 kg/mu of dry leaves are harvested, and the content RA: 13.50 of; STV: 1.55: TSG: 17.2; RA/TSG: 78.4 percent. The economic benefit is increased by 2270 yuan per mu compared with the control.
In summary, in this embodiment, according to the cultivation method of a new stevia rebaudiana variety of this embodiment, in the cultivation method of a new stevia rebaudiana variety provided in this embodiment, parents and parents all adopt the excellent lines cultivated by the inventors to perform selfing, hybridization, group crossing, and breeding of a new stevia rebaudiana variety, the efficiency is high, the bred progeny has stable properties, excellent economic properties, good consistency, and is suitable for direct seeding cultivation of seeds.
The above are only further embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can substitute or change the technical solution of the present invention and its concept within the scope of the present invention.
Claims (10)
1. A cultivation method of a new stevia rebaudiana variety is characterized by comprising the following steps:
step 1: breeding excellent parents; selecting a self-bred excellent strain original field P multiplied by the fragrant and sweet No. 1 combination for hybridization and breeding a Huinong No. 3 strain as a parent 1; selecting the bred excellent variety Huinong No. 3 and Rundd No. 1 to carry out combined hybridization to breed a Huinong No. 9 variety as a parent 2; selecting a cultured excellent strain: dry breeding, carrying out group crossing on the original P, the father P1, the father P2 and the Wangxincg group, and selectively breeding a WPL strain as a parent 3;
step 2: performing group crossing on excellent parents; planting the parent 1, the parent 2 and the parent 3 in a mixed manner, grouping and carrying out group crossing, and performing plant division and seed collection;
and step 3: and (3) carrying out seedling cultivation on the seeds harvested in the plant division manner, selecting an excellent single plant, carrying out field cultivation on the excellent single plant, observing the characters of the whole growth period, testing the yield and the content of dry leaves, and carrying out comprehensive analysis to select the best single plant, namely chloran 12.
2. The breeding method of a new stevia rebaudiana variety as claimed in claim 1, wherein the parent 1 is selected: from the main stevia cultivation area and different growth stages of stevia in China, the following 8 combinations are formed by selecting and confirming excellent single plants according to different growth traits: b04-1 XB 05-4, B04-1 XB 05-6, B04-1 XB 05-and ③ B04-1 XB 06-8, B04-1 XB 06-18, B05-6 XB 06-8, B06-8 XB 06-18 and B05-6 XB 06-18.
3. The method of claim 2, wherein the field cultivation is conducted under isolation conditions, and the seeds are harvested from the parent plant of the grouped sympodial father and the parent plant.
4. The method of claim 3, wherein the characteristics, flowering time, resistance and field comprehensive performance of the seeds are observed and recorded during the seedling cultivation test; when the seeds are subjected to seedling culture and cultivation tests, the leaf yield and the leaf content are tested; the comprehensive analysis compares the selection with the advantages and the disadvantages, and the selection of the excellent selection primarily confirms that B04-1 XB 05-and-c and B05-6 XB 06-18 are excellent combinations.
5. The method of claim 4, wherein the test of seed production and cultivation is carried out continuously for B04-1 XB 05-Jia ③ B05-6 XB 06-18, each index is B05-6 XB 06-18, and finally it is determined that it is a good combination named as Huinongsan and is the parent 1.
6. The breeding method of a new stevia rebaudiana variety as claimed in claim 5, wherein the parent 2 is selected: taking 'Huinong No. 3' as a female parent and 'Rundd No. 1' of self-breeding as a male parent, carrying out hybridization, and harvesting seeds of the male parent and the female parent.
7. The method for cultivating a new species of stevia rebaudiana as claimed in claim 6, wherein the harvested seeds are used for raising seedlings, cultivating, testing, and observing and recording the comprehensive performance of the harvested seeds in the fields such as traits, characteristics, resistance, etc.; testing the leaf yield and leaf content; through comprehensive analysis and comparison, a good single plant F08-6 is selected from seedlings grown from seeds harvested from the parent Rundd No. 1.
8. The method of claim 6, wherein the method comprises the steps of hybridizing Rundd No. 1 as a female parent and F08-6 as a male parent, harvesting seeds from the female parent and using the harvested seeds for growing seedlings, cultivating and testing; observing and recording the characters, characteristics, resistance and field comprehensive performance of the rice; the method comprises the steps of testing the leaf yield and the leaf content, comprehensively analyzing and comparing, selecting an excellent single plant F12-5 from seedlings grown from seeds harvested from a male parent F08-6, carrying out field cultivation test and field popularization planting on the F12-5, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing F12-5 for vigorous growth, stout straws and dark green leaves, and naming F12-5 as a Huinong 9, namely a parent 2, wherein various testing indexes are stable.
9. The breeding method of a new stevia rebaudiana variety as claimed in claim 8, wherein the breeding of the parent 3 comprises: with elite lines of self-breeding germplasm resources: sweet No. 1, Ming P: 19-4, LL: ra19-52, LL: ra19-17, LL: selfing with Ra 19-49;
with elite lines of self-breeding germplasm resources: the original field P: ra19-4 × Dry Breeding: HY;
LL: ra19-49 × Ming P: ra19-4 for combinatorial hybridization;
with elite lines of self-breeding germplasm resources: performing group crossing on dry breeding, wild P, father P1, father P2 and WPL;
with elite lines of self-breeding germplasm resources: the original field P: ra19-4 × Dry Breeding: HY;
LL: ra19-49 × Ming P: ra19-4 for hybridization;
with elite lines of self-breeding germplasm resources: dry breeding, wild P, father P1, father P2, WPL group crossing;
the method comprises the steps of carrying out seed collection, seedling culture and cultivation on the plants, tracking, observing and recording various characters in the whole growth period, testing the yield and the content of dry leaves, comprehensively analyzing and comparing, and selecting a good single plant, namely a parent 3, named as chloran 9 from seedlings grown by seeds harvested from the mass-bred WPL.
10. The method of claim 1, wherein the group is a group of: carrying out group crossing on the group of the parent 1, the parent 2 and the parent 3, carrying out plant collection and seedling cultivation by dividing plants, observing and recording various characters of the whole growth period, and testing the yield and the content of dry leaves; through comprehensive analysis and comparison, excellent single plants are selected from seedlings raised by seeds harvested from parents 3, the excellent single plants are subjected to field cultivation experiments and field popularization planting, various characters in the whole growth period are tracked, observed and recorded, the yield and the content of dry leaves are tested, and the excellent single plants are named as chloran 12.
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| CN1985575A (en) * | 2006-09-27 | 2007-06-27 | 王其斌 | Systemic asexual stevia parent selection breeding process for producing hybrid |
| CN101933454A (en) * | 2010-09-26 | 2011-01-05 | 凤阳县小岗村润农甜叶菊高科有限公司 | Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents |
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| CN1985575A (en) * | 2006-09-27 | 2007-06-27 | 王其斌 | Systemic asexual stevia parent selection breeding process for producing hybrid |
| CN101933454A (en) * | 2010-09-26 | 2011-01-05 | 凤阳县小岗村润农甜叶菊高科有限公司 | Method for culturing excellet stevia rebaudiana seeds by systematically breeding stevia rebaudiana parents |
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