Summary of the invention
The objective of the invention is to deficiency to prior art; Tabernaemontanus bulrush incense wood bits culture medium of edible fungus and preparation method thereof is provided; Utilize edible mushrooms that tabernaemontanus bulrush incense wood provided by the present invention bits culture medium of edible fungus plants not only productive rate height, quality better, abundant nutrients; Fragrant and sweet especially fresh and tender, and have nourishing function and pharmaceutical use.Wherein, the tabernaemontanus bulrush incense wood bits that only are utilized in the fragrant tree of tabernaemontanus bulrush of Dongguan growth prepare culture medium of edible fungus, just can reach above-mentioned technical purpose.
To achieve these goals, the present invention adopts following technical scheme:
Tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 10%-60%
Rice bran 10%-35%
Corn cob 0%-60%
Semen Maydis powder 0%-20%
Cotton seed hulls 0%-20%
Lime carbonate 0.5%-1.5%
Unslaked lime 0.5%-1%.
Preferably, tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 20%-50%
Rice bran 15%-30%
Corn cob 0%-50%
Semen Maydis powder 0%-15%
Cotton seed hulls 0%-15%
Lime carbonate 0.5%-1%
Unslaked lime 0.5%-0.8%.
Preferably, tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 35%
Rice bran 20%
Corn cob 30%
Semen Maydis powder 12.5%
Lime carbonate 1.5%
Unslaked lime 1%.
Preferably, tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 50%
Rice bran 33.5%
Cotton seed hulls 15%
Lime carbonate 1%
Unslaked lime 0.5%.
Preferably, tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it is made up of following raw materials by weight percent:
Tabernaemontanus bulrush incense wood bits 25%
Rice bran 28%
Corn cob 45%
Lime carbonate 1.5%
Unslaked lime 0.5%.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus, it may further comprise the steps:
A, batching: according to the composition of raw materials amount of said tabernaemontanus bulrush incense wood bits culture medium of edible fungus, each component of weighing is also mixed, and obtains compound;
B, spice: said compound is placed stirrer, do earlier and stirred 15 minutes ~ 30 minutes, add the water stirring 15 minutes ~ 30 minutes of wet then, obtain culture material, the moisture content of gained culture material is controlled at 50% ~ 70%;
C, bottling: said culture material is sent to bottling machine by transfer limes, by the bottling machine culture material of packing into automatically in the culture bottle;
D, burrow: utilize piercing tool, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, and big hole is four duck eyes of uniform distribution on every side;
E, capping: steps d utilizes capsuling machine that culture bottle is carried out automatic sealing after finishing;
F, sterilization: step e carries out the HP steam moist heat sterilization to culture bottle after finishing at once, and sterilising temp is 121 ℃ ~ 123 ℃, and sterilization time is 30 minutes ~ 90 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
In the technique scheme, the moisture content of the culture material that obtains among the step b is controlled between 62% ~ 64%.
In the technique scheme, among the step b, the time of said dried stirring is 20 minutes, and the time of said wet stirring is 20 minutes.
In the technique scheme, among the step f, sterilising temp is 121 ℃.
The present invention compared with prior art, beneficial effect is:
The present invention is with the research object of needle mushroom as edible mushrooms.
1) tabernaemontanus bulrush incense wood bits culture medium of edible fungus provided by the invention is because the tabernaemontanus bulrush incense wood bits of setting with the tabernaemontanus bulrush perfume of the growth in the Dongguan replace traditional wood chip; And tabernaemontanus bulrush incense wood bits can provide abundant Mierocrystalline cellulose, semicellulose, xylogen and special flavour ingredient for the growth of edible mushrooms; Thereby the needle mushroom quality better that adopts tabernaemontanus bulrush incense wood bits culture medium of edible fungus to plant out; Rounded and the homogeneous of mushroom cap, the diameter of mushroom cap are about 5mm, and " parachute-opening " phenomenon can not appear in the mushroom cap; The mushroom body is as white as polished jade, and mushroom handle consolidation is not loose.
2) adopt tabernaemontanus bulrush incense wood bits culture medium of edible fungus provided by the invention; Make the productive rate of needle mushroom increase; Adopting effective radical of the needle mushroom that traditional sawdust medium plants out is 200 ~ 400; Effective radical of the needle mushroom that employing tabernaemontanus bulrush incense wood bits culture medium of edible fungus plants out is 400 ~ 600, and therefore, the productive rate of the needle mushroom that employing tabernaemontanus bulrush incense wood bits culture medium of edible fungus plants out increases nearly 1 times.
3) SR of the tabernaemontanus bulrush incense wood provided by the invention bits culture medium of edible fungus needle mushroom of planting out is big, and the average SR of the culture bottle of each 1200mL is 360g.
4) adopt tabernaemontanus bulrush incense wood bits culture medium of edible fungus provided by the invention, make that the biological transformation ratio of needle mushroom is high, biological transformation ratio surpasses 110%; And adopt the biological transformation ratio of the needle mushroom of traditional sawdust medium plantation to be generally 80%; Therefore, the biological transformation ratio of the needle mushroom of tabernaemontanus bulrush incense wood provided by the invention bits culture medium of edible fungus plantation is 1.4 times of biological transformation ratio of the needle mushroom of traditional sawdust medium plantation.
5) needle mushroom that adopts tabernaemontanus bulrush incense wood bits culture medium of edible fungus provided by the invention to plant, fragrant and sweet tender and crisp, abundant nutrients especially is rich in Methionin and l-arginine, is of value to children's brain cell development, has great health care function; And the needle mushroom of being planted contains proflavine, and that proflavine has is anticancer, reducing blood-fat, dietotherapy function and pharmaceutical use such as protect the liver.
6) preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus of the present invention is simple.
Embodiment
Below in conjunction with embodiment the present invention is further described.
embodiment 1.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 35kg, rice bran 20 kg, corn cob 30 kg, Semen Maydis powder 12.5 kg, lime carbonate 1.5kg, unslaked lime 1 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 20 minutes, add the water stirring 20 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 62% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 121 ℃, and sterilization time is 50 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
Wherein, the present invention burrows to the culture material in the culture bottle, mainly is to play the ventilation effect, to promote the growth of edible mushrooms, shortens the growth cycle of edible mushrooms.
Wherein, tabernaemontanus bulrush incense wood bits mainly play the effect of preserving moisture and ventilating in culture medium prescription, for the needle mushroom growth provides Mierocrystalline cellulose, semicellulose, xylogen and special flavour ingredient; Rice bran contains the needle mushroom necessary whole nutrient that grows, for needle mushroom provides crude protein and NFE, and the required C/N ratio of adjustment needle mushroom growth; Corn cob mainly provides carbon source, robust fibre and NFE, and has the effect of suction, water conservation and ventilation; Semen Maydis powder is that the growth of needle mushroom provides crude protein, NFE, robust fibre etc.; Lime carbonate and unslaked lime are mainly used in the pH value of regulating substratum, and calcium ion are provided for the growth of needle mushroom.
Wherein, the pressure in the HP steam moist heat sterilization mentioned of the present invention is 1Kg/cm
2~ 1.25Kg/cm
2
embodiment 2.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 50kg, rice bran 33.5 kg, cotton seed hulls 15 kg, lime carbonate 1kg, unslaked lime 0.5 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 15 minutes, add the water stirring 15 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 64% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 122 ℃, and sterilization time is 30 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 3.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 25kg, rice bran 28 kg, corn cob 45 kg, lime carbonate 1.5 kg, unslaked lime 0.5 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 25 minutes, add the water stirring 30 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 63% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 123 ℃, and sterilization time is 40 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 4.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 35kg, rice bran 18.5 kg, corn cob 30kg, Semen Maydis powder 15 kg, lime carbonate 1kg, unslaked lime 0.5 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 30 minutes, add the water stirring 25 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 62.5% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 121 ℃, and sterilization time is 90 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 5.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 50kg, rice bran 32.5 kg, cotton seed hulls 15.5 kg, lime carbonate 1kg, unslaked lime 1 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 20 minutes, add the water stirring 15 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 63.5% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 123 ℃, and sterilization time is 60 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 6.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 25kg, rice bran 24kg, corn cob 50 kg, lime carbonate 0.5kg, unslaked lime 0.5 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 20 minutes, add the water stirring 15 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 70% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 122 ℃, and sterilization time is 70 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 7.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 60kg, rice bran 10 kg, Semen Maydis powder 20 kg, cotton seed hulls 8 kg, lime carbonate 1.4kg, unslaked lime 0.6 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 20 minutes, add the water stirring 15 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 50% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 123 ℃, and sterilization time is 45 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 8.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 10kg, rice bran 28.6 kg, corn cob 60 kg, lime carbonate 0.8kg, unslaked lime 0.6 kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 15 minutes, add the water stirring 20 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 64% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 121 ℃, and sterilization time is 80 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
embodiment 9.
The preparation method of tabernaemontanus bulrush incense wood bits culture medium of edible fungus is following:
Take by weighing tabernaemontanus bulrush incense wood bits 40kg, rice bran 20.7 kg, cotton seed hulls 20 kg, Semen Maydis powder 18 kg, lime carbonate 0.8kg, unslaked lime 0.5kg, load weighted each component is mixed, obtain compound.Compound is placed stirrer, do earlier and stirred 20 minutes, add the water stirring 26 minutes of wet then, obtain culture material, wherein, the moisture content of culture material is 63% of a culture material total amount.Culture material is sent to bottling machine by transfer limes, and by the bottling machine culture material of packing into automatically in the culture bottle, wherein, the culture bottle of each 1200mL is packed into, and gross weight is 860g (it is heavy to contain bottle) behind the culture material.Utilize piercing tool then, the culture material in the culture bottle is burrowed to the material end from charge level, make a call to five holes altogether, wherein, the culture material center is a big hole, four duck eyes of uniform distribution around the big hole; Utilize capsuling machine that culture bottle is carried out automatic sealing then.After the bottling capping, utilize the autoclaving stove to carry out the HP steam moist heat sterilization to culture bottle at once, sterilising temp is 121.5 ℃, and sterilization time is 60 minutes; Sterilization treats that culture bottle is cooled to below 20 ℃ after finishing, and promptly obtains tabernaemontanus bulrush incense wood bits culture medium of edible fungus.
Should be noted that at last; Above embodiment is only in order to explain technical scheme of the present invention; But not to the restriction of protection domain of the present invention, although with reference to preferred embodiment the present invention has been done explanation at length, those of ordinary skill in the art is to be understood that; Can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the essence and the scope of technical scheme of the present invention.