CN102078492A - Preparation method and application of compound white paeony root preparation - Google Patents
Preparation method and application of compound white paeony root preparation Download PDFInfo
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- CN102078492A CN102078492A CN2011100087980A CN201110008798A CN102078492A CN 102078492 A CN102078492 A CN 102078492A CN 2011100087980 A CN2011100087980 A CN 2011100087980A CN 201110008798 A CN201110008798 A CN 201110008798A CN 102078492 A CN102078492 A CN 102078492A
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Abstract
The invention belongs to the technical field of medical product research and development, and relates to a preparation method and application of a compound white paeony root preparation. The preparation method comprises the following steps of: selecting and washing 6 to 9 g of high-quality white paeony root, 4 to 6 g of lucid ganoderma, 4 to 6 g of astragalus mongholicus, 4 to 6 g of ginseng, 4 to 6 g of honeysuckle, 4 to 6 g of andrographis, 4 to 6 g of baical skullcap root, 4 to 6 g of acorus calamus, 4 to 6 g of isatis root, 2 to 4 g of rhubarb, 2 to 4 g of phellodendron, 2 to 4 g of dandelion, 2 to 4 g of sophora flavescens, 2 to 4 g of garlic and 2 to 4 g of liquorice root; drying by using microwave; crushing into 50-mesh powder; adding water, boiling and extracting for 3 times; combining extracting solution; filtering to obtain a filtrate; concentrating the filtrate into an extractum; mixing the extractum and starch in a ratio of 1:4; granulating by using a 10-mesh sieve; and drying and screening by using a 14-mesh sieve to obtain the compound white paeony root preparation. The product has the effects of promoting the liver function and the protein metabolism of fancy carps. The product contributes to industrial production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of preparation method and its usage of compound recipe Radix Paeoniae Alba preparation.Product of the present invention has the Cyprinus carpio L. liver function of promotion and protein metabolism effect, and product of the present invention is beneficial to suitability for industrialized production.
Background technology
Advantage such as that Chinese herbal feed additive has is natural, economic, efficient, noresidue, no Drug resistance and side effect are little, and have the dual function of medical and trophism concurrently, the drawback of no antibiotics and chemicals, be widely used in recent years in the aquaculture of aquatic animal, in order to prevent disease, growth promotion with improve aquatic product quality.Contain the various active composition in the Chinese herbal medicine, the scalable immunity function of animal.Therefore mainly can to improve the animal body immunologic function relevant with it to it is generally acknowledged Chinese herbal medicine prevent disease and growth promoting function.The feed additive of Fish has shortcomings such as easy thawing, poor stability at present, and a part can not be eaten by fish, and utilization rate is low, and waste is serious.Glutamate pyruvate transaminase (GPT), glutamic oxaloacetic transaminase, GOT (GOT) are aminotransferases important in the body protein metabolic process, and the active height of the two has reflected the situation of protein metabolism and liver function in the liver.The transaminase mainly is distributed in the cell under the normal condition, has a large amount of transaminases and be released into blood when liver dysfunction in hepatocyte, makes transaminase activity rising in the serum, and transaminase activity reduces in the hepatocyte simultaneously.Compound recipe Radix Paeoniae Alba preparation can make that transaminase activity generally raises in the Cyprinus carpio L. liver, illustrates that compound recipe Radix Paeoniae Alba preparation does not have hepatotoxicity and the liver function and the protein metabolism of Cyprinus carpio L. had facilitation.
Summary of the invention
1, a kind of preparation method of compound recipe Radix Paeoniae Alba preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Radix Paeoniae Alba 6g-9g, Ganoderma 4g-6g, Radix Astragali 4g-6g, Radix Ginseng 4g-6g, Flos Lonicerae 4g-6g, Herba Andrographis 4g-6g, Radix Scutellariae 4g-6g, Rhizoma Acori Graminei 4g-6g, Radix Isatidis 4g-6g, Radix Et Rhizoma Rhei 2g-4g, Cortex Phellodendri 2g-4g, Herba Taraxaci 2g-4g, Radix Sophorae Flavescentis 2g-4g, Bulbus Allii 2g-4g, Radix Glycyrrhizae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Radix Paeoniae Alba preparation.
2, the purposes of a kind of compound recipe Radix Paeoniae Alba preparation as claimed in claim 1 is characterised in that it has the Cyprinus carpio L. fish liver function of promotion and protein metabolism effect.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
The preparation method and its usage that relates to a kind of compound recipe Radix Paeoniae Alba preparation of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): select high-quality Radix Paeoniae Alba 6g, Ganoderma 4g, Radix Astragali 4g, Radix Ginseng 4g, Flos Lonicerae 4g, Herba Andrographis 4g, Radix Scutellariae 4g, Rhizoma Acori Graminei 4g, Radix Isatidis 4g, Radix Et Rhizoma Rhei 2g, Cortex Phellodendri 2g, Herba Taraxaci 2g, Radix Sophorae Flavescentis 2g, Bulbus Allii 2g, Radix Glycyrrhizae 2g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6 times of water gagings first and soak 4.0h, heated and boiled is extracted 2.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Radix Paeoniae Alba preparation.
Embodiment 2:
Step (one): select high-quality Radix Paeoniae Alba 9g, Ganoderma 6g, Radix Astragali 6g, Radix Ginseng 6g, Flos Lonicerae 6g, Herba Andrographis 6g, Radix Scutellariae 6g, Rhizoma Acori Graminei 6g, Radix Isatidis 6g, Radix Et Rhizoma Rhei 4g, Cortex Phellodendri 4g, Herba Taraxaci 4g, Radix Sophorae Flavescentis 4g, Bulbus Allii 4g, Radix Glycyrrhizae 4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, get filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Radix Paeoniae Alba preparation.
Pharmacological evaluation
Compound recipe Radix Paeoniae Alba preparation promotes Cyprinus carpio L. liver function and protein metabolism effect experiment
1 materials and methods
1.1 material
1.1.1 compound recipe Radix Paeoniae Alba preparation
Compound recipe Radix Paeoniae Alba preparation consists of: Radix Paeoniae Alba 9g, Ganoderma 6g, Radix Astragali 6g, Radix Ginseng 6g, Flos Lonicerae 6g, Herba Andrographis 6g, Radix Scutellariae 6g, Rhizoma Acori Graminei 6g, Radix Isatidis 6g, Radix Et Rhizoma Rhei 4g, Cortex Phellodendri 4g, Herba Taraxaci 4g, Radix Sophorae Flavescentis 4g, Bulbus Allii 4g, Radix Glycyrrhizae 4g, compound recipe Radix Paeoniae Alba preparation composition is established three groups of Concentraton gradient, promptly on each flavor dose basis, reduce or increase by 25% crude drug amount, be prepared into basic, normal, high dosage group.Form three groups of Concentraton gradient by compound recipe Radix Paeoniae Alba preparation and select the high-quality Radix Paeoniae Alba, Ganoderma, the Radix Astragali, Radix Ginseng, Flos Lonicerae, Herba Andrographis, Radix Scutellariae, Rhizoma Acori Graminei, Radix Isatidis, Radix Et Rhizoma Rhei, Cortex Phellodendri, Herba Taraxaci, Radix Sophorae Flavescentis, Bulbus Allii, Radix Glycyrrhizae is also cleaned respectively, microwave drying, pulverizing is 50 order powder, every group of powder added water boil to be extracted 3 times, add 8 times of water gagings first and soak 6.0h, heated and boiled is extracted 3.0h, add for the second time 4 times of water gaging heated and boiled and extract 2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.5h, merge extractive liquid,, filter, filtrate, filtrate is condensed into extractum, in extractum: starch=1: 4 ratio mixing, granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Radix Paeoniae Alba preparation.
1.1.2 experiment feedstuff
By compound recipe Radix Paeoniae Alba preparation: the mixed uniform preparation of normal feedstuff=3: 5 is a compound recipe Radix Paeoniae Alba preparation experiment feedstuff, does blank with normal feedstuff.Experiment normal feedstuff prescription sees Table 1.
Table 1 experiment is filled a prescription with normal feedstuff
1.2 method
1.2.1 experiment is raised with Cyprinus carpio L.
Experiment uses Cyprinus carpio L. Cyprinus carpio L. available from Aquatic product market, Weihai, body weight (41.1 ± 5.0) g, and long (10.27 ± 0.48) cm of body, the male and female dual-purpose is supported in indoor glass aquarium before the experiment beginning temporarily, with commercial feed it is satiated with food and throws something and feeds.As source of recycled water, 24h continues aeration aerating, keeps dissolved oxygen in water amount 5.0mg/L with the tap water of abundant aeration dechlorination, and water temperature is controlled at (21 ± 1) ℃, raises and train to begin after 7 days to test.Experiment is divided into 2 groups with Cyprinus carpio L. at random by body weight, and experimental group is with compound recipe Radix Paeoniae Alba preparation experiment feedstuff feeding, the matched group normal feedstuff of throwing something and feeding, every group 20 tail, establish 3 parallel.Experiment periods every other day feeding volume is 2%～3% of a fish body constitution amount, and regularly throw something and feed during 7:00 and during 19:00 every day, and be 15 days experimental period.
1.2.2 measure the collection of sample
The experiment end began jejunitas 24h in preceding 1 day, got 5 tail fishes at random from each aquarium, dissected and got liver, and is stand-by.
1.2.3 the mensuration of liver biochemical indexes
Get liver organization, with the clean back of 4 ℃ normal saline flushings absorbent paper suck dry moisture, weigh, get the 0.5g tissue, add the normal saline homogenate of 9 times of volumes, the centrifugal 15min of 3000r/min gets liver tissue homogenate's supernatant of 10%, with normal saline dilution is 1% homogenate, measures related biochemical indicator.
(1) gpt activity (GPT): the reitman-frankel method that adopts GPT test kit (bio-engineering research institute, lot number 20101112 are built up in Nanjing) to provide is measured.
(2) glutamic oxaloacetic transaminase, GOT (GOT): the reitman-frankel method that adopts GOT test kit (bio-engineering research institute, lot number 20101111 are built up in Nanjing) to provide is measured.
2 results
Compound recipe Radix Paeoniae Alba preparation is to glutamate pyruvate transaminase (GPT), the active result that influences of glutamic oxaloacetic transaminase, GOT (GOT) in the Cyprinus carpio L. liver
See Table 2.
Table 2 compound recipe Radix Paeoniae Alba preparation is to the result that influences of glutamate pyruvate transaminase, glutamic oxaloacetic transaminase, GOT in the Cyprinus carpio L. liver
Annotate: compare * P＜0.05 with matched group
By table 2 as seen, compare with matched group, can the raise activity of glutamate pyruvate transaminase in the Cyprinus carpio L. liver, glutamic oxaloacetic transaminase, GOT of compound recipe Radix Paeoniae Alba preparation, the glutamic oxaloacetic transaminase, GOT effect is remarkable in the basic, normal, high dosage group of the compound recipe Radix Paeoniae Alba preparation rising Cyprinus carpio L. liver, the glutamate pyruvate transaminase effect is remarkable in the middle and high dosage group of the compound recipe Radix Paeoniae Alba preparation rising Cyprinus carpio L. liver, illustrates that compound recipe Radix Paeoniae Alba preparation does not have hepatotoxicity and the liver function and the protein metabolism of Cyprinus carpio L. had facilitation.
Claims (2)
1. the preparation method of a compound recipe Radix Paeoniae Alba preparation is characterized in that the preparation method of said preparation comprises following process:
Step (one): select high-quality Radix Paeoniae Alba 6g-9g, Ganoderma 4g-6g, Radix Astragali 4g-6g, Radix Ginseng 4g-6g, Flos Lonicerae 4g-6g, Herba Andrographis 4g-6g, Radix Scutellariae 4g-6g, Rhizoma Acori Graminei 4g-6g, Radix Isatidis 4g-6g, Radix Et Rhizoma Rhei 2g-4g, Cortex Phellodendri 2g-4g, Herba Taraxaci 2g-4g, Radix Sophorae Flavescentis 2g-4g, Bulbus Allii 2g-4g, Radix Glycyrrhizae 2g-4g and clean, microwave drying, pulverizing are 50 order powder;
Step (two): the powder in the step () is added water boil extract 3 times, add 6-8 times of water gaging first and soak 4.0h-6.0h, heated and boiled is extracted 2.0h-3.0h, add for the second time 4 times of water gaging heated and boiled and extract 1.5h-2.0h, add 3 times of water gaging heated and boiled for the third time and extract 1.0h-1.5h, merge extractive liquid, filters, and gets filtrate;
Step (three): the filtrate in the step (two) is condensed into extractum, in extractum: starch=1: 4 ratio mixing, to granulate with 10 mesh sieves, the oven dry back is crossed 14 mesh sieves and is promptly got compound recipe Radix Paeoniae Alba preparation.
2. the purposes of a kind of compound recipe Radix Paeoniae Alba preparation as claimed in claim 1 is characterised in that it has the Cyprinus carpio L. fish liver function of promotion and protein metabolism effect.
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| CN2011100087980A CN102078492B (en) | 2011-01-03 | 2011-01-03 | Preparation method and application of compound white paeony root preparation |
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| CN2011100087980A CN102078492B (en) | 2011-01-03 | 2011-01-03 | Preparation method and application of compound white paeony root preparation |
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| CN102078492B CN102078492B (en) | 2012-04-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103315160A (en) * | 2013-07-13 | 2013-09-25 | 肖玲 | Special feed additive for sturgeon |
| CN103340316A (en) * | 2013-07-13 | 2013-10-09 | 肖玲 | Special compound feed for sturgeon, and preparation method thereof |
| CN104770612A (en) * | 2015-04-21 | 2015-07-15 | 长沙市睿圣泽生态农业科技有限公司 | Chinese-herbal-medicine fish feed additive with effects of preventing diseases and promoting growth and processing method thereof |
| CN110478405A (en) * | 2019-09-06 | 2019-11-22 | 广东广盈生物技术有限公司 | Nourishing and protecting liver Chinese medicine composition and its preparation and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644077A (en) * | 2005-01-11 | 2005-07-27 | 冯绍宽 | Plant additive premixed forage for aquatic culture and bait therefrom |
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2011
- 2011-01-03 CN CN2011100087980A patent/CN102078492B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1644077A (en) * | 2005-01-11 | 2005-07-27 | 冯绍宽 | Plant additive premixed forage for aquatic culture and bait therefrom |
Non-Patent Citations (1)
| Title |
|---|
| 《北京水产》 20080430 禇衍伟，张太娥 中草药饲料添加剂在鱼类养殖中的应用（综述） 57-60 1-2 , 第113期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103315160A (en) * | 2013-07-13 | 2013-09-25 | 肖玲 | Special feed additive for sturgeon |
| CN103340316A (en) * | 2013-07-13 | 2013-10-09 | 肖玲 | Special compound feed for sturgeon, and preparation method thereof |
| CN103315160B (en) * | 2013-07-13 | 2014-04-09 | 肖玲 | Special feed additive for sturgeon |
| CN103340316B (en) * | 2013-07-13 | 2014-07-16 | 肖玲 | Special compound feed for sturgeon, and preparation method thereof |
| CN104770612A (en) * | 2015-04-21 | 2015-07-15 | 长沙市睿圣泽生态农业科技有限公司 | Chinese-herbal-medicine fish feed additive with effects of preventing diseases and promoting growth and processing method thereof |
| CN110478405A (en) * | 2019-09-06 | 2019-11-22 | 广东广盈生物技术有限公司 | Nourishing and protecting liver Chinese medicine composition and its preparation and application |
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| CN102078492B (en) | 2012-04-18 |
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