CN110478405A - Nourishing and protecting liver Chinese medicine composition and its preparation and application - Google Patents
Nourishing and protecting liver Chinese medicine composition and its preparation and application Download PDFInfo
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- CN110478405A CN110478405A CN201910840292.2A CN201910840292A CN110478405A CN 110478405 A CN110478405 A CN 110478405A CN 201910840292 A CN201910840292 A CN 201910840292A CN 110478405 A CN110478405 A CN 110478405A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/233—Bupleurum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/76—Salicaceae (Willow family), e.g. poplar
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Abstract
The present invention relates to a kind of nourishing and protecting liver Chinese medicine composition and its preparations and application.The effective component of the Chinese medicine composition is prepared by the raw material of following parts by weight: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 20 parts of -80 parts of poplar flowers, 20 parts of -60 parts of Chinese herbaceous peonies, 0 part of -60 parts of Radix Astragali, 0 part of -80 parts of dandelion, 0 part of -50 parts of radix scutellariae, 0 part of -60 parts of radix bupleuri, 0 part of -60 parts of rheum officinale.The nourishing and protecting liver composition of the composition of raw materials can protect aquiculture animal liver injury-free or mitigate damage, avoid and cause Other diseases because of hepar damnification, reduce the generation of aquaculture creature disease on the whole.Meanwhile using plant-derived composition of raw materials, the problem of environmental pollution and drug resistance is not present, toxic side effect is small, safely and effectively, convenient for the use of modern high density, intensive culture mode.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to nourishing and protecting liver Chinese medicine composition and its preparation and answer
With.
Background technique
" hepatopathy " (The liver disease) is that a kind of common and harm is biggish non-infectious in aquaculture in recent years
Property, hepar damnification, hepatosis disease caused by non-parasitic sexual factor, it have become sea, the main cultured fishes of fresh water,
One of serious diseases of aquiculture animals such as shrimps, crab class.After aquiculture animal suffers from liver diseases, less serious case's appetite subtracts
It moves back, slow growth, the severe one death rate can reach 50%-60%.When body occur hepatopathy after, it is easy to due to hypoimmunity after
Send out other diseases.Although simple liver class disease is without infectiousness, it is endangered and consequence is often considerably beyond communicable disease, and
And the extent of injury of communicable disease can be aggravated, increase the illness probability of cultivated animals.In particular with feeding people for water
The demand of cultured product higher and higher today is produced, raisers constantly pursue higher cultivation density, and intensive high density is supported
Grow, feed artifical compound feed, it is lack of standardization using drug, the deteriorating increasingly of water body environment, in feed nutritional ingredient unbalance and
There are the adverse effects factor such as noxious material is more and more in missing and feed, all may to aquiculture animal (such as fish,
Shrimp, crab etc.) liver damage, cause the generation of the aquatic livestocks hepatopathy such as current cultivation fish shrimp crab extremely universal, cause to support
It is also increasingly severe to grow aquatic livestock hepatic injury phenomenon.
Currently, the using effect of the nourishing and protecting liver drug of aquiculture animal is to be improved, it cannot very high prevention and treatment liver
Damage.Therefore, developing a kind of drug that nourishing and protecting liver effect is good for aquiculture animal seems particularly significant.
Summary of the invention
Based on this, the main object of the present invention is to provide a kind of nourishing and protecting liver Chinese medicine composition.The nourishing and protecting liver composition
Aquiculture animal liver can be protected injury-free or mitigate damage, avoid and cause Other diseases because of hepar damnification,
Reduce the generation of aquaculture creature disease on the whole.
The purpose of the present invention is what is be achieved through the following technical solutions:
Present invention generally provides a kind of nourishing and protecting liver Chinese medicine composition, the effective component of the Chinese medicine composition is by following
The raw material of parts by weight is prepared: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 20 parts of -80 parts of poplars
Tree flower, 20 parts of -60 parts of Chinese herbaceous peonies, 0 part of -60 parts of Radix Astragali, 0 part of -80 parts of dandelion, 0 part of -50 parts of radix scutellariae, 0 part of -60 parts of radix bupleuri, 0 part -
60 parts of rheum officinales.
In one of the embodiments, the effective component of the Chinese medicine composition be by following parts by weight raw material preparation and
At: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 45 parts of -75 parts of poplar flowers, 23 parts of -48 parts of Chinese herbaceous peonies
Medicine, 14 parts of -48 parts of Radix Astragalis, 45 parts of -78 parts of dandelions, 20 parts of -45 parts of radix scutellariaes, 37 parts of -55 parts of radix bupleuri and 36 parts of -58 parts of rheum officinales.
In one of the embodiments, the effective component of the Chinese medicine composition be by following parts by weight raw material preparation and
At: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 45 parts of -60 parts of poplar flowers, 35 parts of -45 parts of Chinese herbaceous peonies
Medicine, 30 parts of -48 parts of Radix Astragalis, 50 parts of -70 parts of dandelions, 25 parts of -36 parts of radix scutellariaes, 43 parts of -55 parts of radix bupleuri and 36 parts of -50 parts of rheum officinales.
It is a further object of the present invention to provide a kind of preparation method of above-mentioned nourishing and protecting liver Chinese medicine composition, the preparations
Method includes the following steps:
(1) raw material is taken, is added water to cook, filters, obtains that water mentions filtrate and water mentions filter residue；
(2) it takes the water to mention filter residue, adds alcohol solution dipping, diacolation obtains alcohol extracting filtrate；
(3) ethyl alcohol in the alcohol extracting filtrate is removed, products therefrom is mentioned into filtrate with the water and is mixed and to gained mixing
Liquid is concentrated.
In one of the embodiments, in step (1), the weight of the water is 8 times -18 times of the weight of the raw material,
The number of the decoction is 1 time -2 times, each 2h-4h.
In one of the embodiments, in step (2), the ethanol solution is the second that percent by volume is 50%-70%
Alcohol solution, the weight of the ethanol solution are 4 times -8 times of the weight that the water mentions filter residue, and the time of the immersion is 40h-
55h。
In one of the embodiments, in step (2), the flow velocity of the diacolation is 20ml/min-30ml/min.
In one of the embodiments, in step (3), the mode of the concentration uses rotary evaporation, and products therefrom is concentrated
Volume be the mixeding liquid volume 1/3-2/3.
A further object of the invention is to provide a kind of above-mentioned composition in the drug of preparation maintenance aquiculture animal liver
In application.
The drug is liquid medicine in one of the embodiments,.
The aquiculture animal is shrimp, fish or crab in one of the embodiments,.
Compared with prior art, the present invention have it is following the utility model has the advantages that
Poplar flower and Chinese herbaceous peony and honeysuckle, Fructus Forsythiae, Radix Glycyrrhizae etc. are constituted specific raw materials of traditional Chinese medicine composition by the present invention together
Formula.The nourishing and protecting liver composition of the composition of raw materials can protect aquiculture animal liver injury-free or mitigate damage,
It avoids and causes Other diseases because of hepar damnification, reduce the generation of aquaculture creature disease on the whole.Meanwhile using plant
The problem of material resource composition of raw materials, there is no environmental pollution and drug resistances, toxic side effect is small, safely and effectively, convenient for modern highly dense
The use of degree, intensive culture mode.
Detailed description of the invention
Fig. 1 is that Penaeus Vannmei feeds the forward and backward comparison diagram of embodiment drug；
Fig. 2 is that yellow bone fish feeds the forward and backward comparison diagram of embodiment drug.
Specific embodiment
It to facilitate the understanding of the present invention, below will be to invention is more fully described.But the present invention can be to be permitted
Mostly different form is realized, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes
It is more thorough and comprehensive to the understanding of the disclosure.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
The present embodiment provides a kind of nourishing and protecting liver Chinese medicine composition, the effective component of the Chinese medicine composition is by following weight
The raw material of part is prepared: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 20 parts of -80 parts of poplars
Flower, 20 parts of -60 parts of Chinese herbaceous peonies, 0 part of -60 parts of Radix Astragali, 0 part of -80 parts of dandelion, 0 part of -50 parts of radix scutellariae, 0 part of -60 parts of radix bupleuri, 0 part -60
Part rheum officinale.
In the nourishing and protecting liver Chinese medicine composition of the present embodiment, honeysuckle it is most important effect be it is clearing heat and detoxicating, have reducing liver heat
Effect.Be directed to the pathological analysis of honeysuckle, it have the function of it is antiviral, can mitigate mitigate liver pathologic wound
Evil.In the treatment of hepatopathy, honeysuckle is primarily adapted for use in excessive noxious heat or the damp and hot hepatopathy for stopping up Sheng and secondary infection person.Honeysuckle
In triterpenoid saponin again have good protective effect to the damage of liver, wherein fulvoushair honeysuckle flower saponin(e can reduce hepar damnification
Severity, therefore honeysuckle has good protective effect to liver in terms of pharmacology.Fructus Forsythiae not only can be clearing heat and detoxicating,
Liver can also be protected, it can repair impaired liver cell, and can form protective film in liver surface, and virus can be prevented to produce liver
Raw injury, in addition it can also accelerate hepatic glycogen generation, improve the function of detoxification of liver.Wang Wensheng etc. passes through studies have shown that Fructus Forsythiae
Decocting liquid can obviously inhibit serum glutamic pyruvic transminase activity to increase, while mitigate liver denaturation and necrosis, the pier fruit in Fructus Forsythiae
Acid, ursolic acid are the effective component of Fructus Forsythiae anti-liver injury.Active material in Radix Glycyrrhizae is to carbon tetrachloride and chemical carcinogens methyl
Hepatic injury caused by azobenzene and liver cancer have significant protective effect, and liver denaturation and necrosis can be made to mitigate, and hepatic glycogen restores, and subtracts
Light liver cell fatty degeneration necrosis, reduces serum transaminase vigor, increases the content of glycogen and RNA in liver cell, promote liver cell again
It is raw.In addition, part of it anti-inflammatory effect is realized by the effect of glucocorticoid possessed by Radix Glycyrrhizae.Pass through anti-inflammatory shield
Liver achievees the purpose that drop enzyme and improves liver function, hepatic lesion and abnormal liver function caused by clinical all reasons can be applied sweet
Grass.Poplar flower have the function of it is clearing heat and detoxicating and it is disease-resistant rush down poison, reduce liver load, prevention and control perihepatitis, the spy for improving immunity
Point.The more glycosides of Chinese herbaceous peony in Chinese herbaceous peony have antioxidation, mitigate damage of the cell factor to liver, block NO and iNOS to liver
Damage, the hepatic injury for blocking TXA2 to mediate.
In a preferred embodiment, the effective component of the Chinese medicine composition be by following parts by weight raw material preparation and
At: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 45 parts of -75 parts of poplar flowers, 23 parts of -48 parts of Chinese herbaceous peonies
Medicine, 14 parts of -48 parts of Radix Astragalis, 45 parts of -78 parts of dandelions, 20 parts of -45 parts of radix scutellariaes, 37 parts of -55 parts of radix bupleuri and 36 parts of -58 parts of rheum officinales.
In a preferred embodiment, the effective component of the Chinese medicine composition be by following parts by weight raw material preparation and
At: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 45 parts of -60 parts of poplar flowers, 35 parts of -45 parts of Chinese herbaceous peonies
Medicine, 30 parts of -48 parts of Radix Astragalis, 50 parts of -70 parts of dandelions, 25 parts of -36 parts of radix scutellariaes, 43 parts of -55 parts of radix bupleuri and 36 parts of -50 parts of rheum officinales.
The embodiment of the present invention also provides the preparation method of above-mentioned nourishing and protecting liver Chinese medicine composition, which includes such as
Lower step:
(1) raw material is taken, is added water to cook, filters, obtains that water mentions filtrate and water mentions filter residue；
(2) it takes the water to mention filter residue, adds alcohol solution dipping, diacolation obtains alcohol extracting filtrate；
(3) ethyl alcohol in the alcohol extracting filtrate is removed, products therefrom is mentioned into filtrate with the water and is mixed and to gained mixing
Liquid is concentrated.
In one embodiment, in step (1), the weight of the water is 8 times -12 times of the weight of the raw material, is decocted
Number be 2 times, each 2h-4h.
In one embodiment, in step (2), the ethanol solution is the ethanol water that percent by volume is 50%-70%
Solution, the weight of the ethanol solution are 4 times -8 times of the weight that the water mentions filter residue, and the time of immersion is 40h-55h.
In one embodiment, in step (2), the flow velocity of the diacolation is 20ml/min-30ml/min.It is understood that
It is that diacolation is completed using percolator.
In one embodiment, in step (3), the mode of the concentration uses rotary evaporation, and the body of products therefrom is concentrated
Product is the 1/3-2/3 of the mixeding liquid volume.
The present embodiment also provides application of the combinations of the above object in the drug of preparation maintenance aquiculture animal liver.This
The drug of embodiment maintenance aquiculture animal liver can be the drug for preventing and treating liver pancreas syndrome, be also possible to other
The protective agents for liver pancreas damage phenomenon.
In one embodiment, the drug is liquid medicine.It is understood that the composition of the present embodiment can be made
For at any pharmaceutical dosage form suitable for feeding.But for the ease of absorbing, when being used for aquiculture animal, it is prepared into liquid medicine
It more easily absorbs.
In one embodiment, which is shrimp or fish.Such as Penaeus Vannmei, yellow bone fish etc..
Embodiment 1
The present embodiment provides a kind of Chinese medicinal aquas and preparation method thereof for conserving aquiculture animal liver.The Chinese medicinal aqua
Medicinal raw material formula referring to table 1.The preparation method of the Chinese medicinal aqua includes the following steps:
(1) medicine material is weighed according to the composition of raw materials of table 1, is extracted with the water of 8 times of medicinal raw material weight, extract 2
Secondary, 2 hours every time, filtration obtained that water mentions filter residue and water mentions filtrate, will extract gained filtrate twice and merge.
(2) ethanol water that dose volume percentage is 60%, the ethanol water of filter residue is mentioned with 4 times of step (1) water
Soaking water mentions filter residue, is added in percolator, impregnates 48 hours, carries out diacolation later, and flow velocity 25ml/min is received until terminating
Collect percolate, and by filtrate recycling ethanol.
(3) alcohol extracting filtrate (filtrate after recycling ethyl alcohol) mixing that the water of step 1 is mentioned to filtrate and step 2, is steamed with rotation
Hair instrument is concentrated into 2/3rds of total volume.
Embodiment 2
The present embodiment provides a kind of Chinese medicinal aquas and preparation method thereof for conserving aquiculture animal liver.The Chinese medicinal aqua
Medicinal raw material formula referring to table 1.The preparation method of the Chinese medicinal aqua includes the following steps:
(1) medicine material is weighed according to the composition of raw materials of table 1, is extracted with the water of 10 times of medicinal raw material weight, extract 2
Secondary, 3 hours every time, filtration obtained that water mentions filter residue and water mentions filtrate.
(2) ethanol water that dose volume percentage is 50%, the ethanol water of filter residue is proposed with 6 times of weight step (1) water
Solution soaking water mentions filter residue, is added in percolator, impregnates 40 hours, carries out diacolation, flow velocity 20ml/min, Zhi Daojie later
Beam collects percolate, and by filtrate recycling ethanol.
(3) alcohol extracting filtrate (filtrate after recycling ethyl alcohol) mixing that the water of step 1 is mentioned to filtrate and step 2, is steamed with rotation
Hair instrument is concentrated into the half of total volume.
Embodiment 3
The present embodiment provides a kind of Chinese medicinal aquas and preparation method thereof for conserving aquiculture animal liver.The Chinese medicinal aqua
Medicinal raw material formula referring to table 1.The preparation method of the Chinese medicinal aqua includes the following steps:
(1) medicine material is weighed according to the composition of raw materials of table 1, is extracted with the water of 12 times of medicinal raw material weight, extract 2
Secondary, 4 hours every time, filtration obtained that water mentions filter residue and water mentions filtrate, will extract gained filtrate twice and merge.
(2) ethanol water that dose volume percentage is 70%, the ethanol water of filter residue is mentioned with 8 times of step (1) water
Soaking water mentions filter residue, is added in percolator, impregnates 55 hours, carries out diacolation later, and flow velocity 30ml/min is received until terminating
Collect percolate, and by filtrate recycling ethanol.
(3) alcohol extracting filtrate (filtrate after recycling ethyl alcohol) mixing that the water of step 1 is mentioned to filtrate and step 2, is steamed with rotation
Hair instrument is concentrated into the one third of total volume.
Embodiment 4
The present embodiment is the change case of embodiment 1, also provide it is a kind of conserve aquiculture animal liver Chinese medicinal aqua and
Preparation method.Relative to the composition of raw materials difference for preparing Chinese medicinal aqua is essentially consisted in place of the variation of embodiment 1, referring to table 1.
The preparation method is the same as that of Example 1.
Embodiment 5
The present embodiment is the change case of embodiment 1, also provide it is a kind of conserve aquiculture animal liver Chinese medicinal aqua and
Preparation method.Relative to the composition of raw materials difference for preparing Chinese medicinal aqua is essentially consisted in place of the variation of embodiment 1, referring to table 1.
The preparation method is the same as that of Example 1.
Embodiment 6
The present embodiment is the change case of embodiment 1, also provide it is a kind of conserve aquiculture animal liver Chinese medicinal aqua and
Preparation method.Relative to the composition of raw materials difference for preparing Chinese medicinal aqua is essentially consisted in place of the variation of embodiment 1, referring to table 1.
The preparation method is the same as that of Example 1.
Table 1
Comparative example 1
This comparative example is the comparative example of embodiment 1, relative to embodiment 1 difference mainly do not contain poplar flower and
Chinese herbaceous peony.
Comparative example 2
This comparative example is the comparative example of embodiment 1, and the difference relative to embodiment 1 mainly contains 5 parts of poplar flowers
With 10 portions of Chinese herbaceous peonies.
Measure of merit, to Penaeus Vannmei liver pancreas injury repair Effect study
1, South America prawn repairs background, method
(1) Guangdong Province Mazhang District farm
Cultivate situation: pond size is between 2 mu -3 mu, and 0.8 meter -1.0 meters of the depth of water, discovery on March 6th, 2019 South America is white
Prawn liver is fuzzy, and stomach is not full, and material feeding is slow, has 2 jin of seedlings to break off the base daily, chooses wherein two mouthfuls of ponds and is used as experiment pond.
Rearing conditions: dissolved oxygen in water is greater than 7mg/L, and pH value is 7.80 ± 0.5.
Example 1 group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, comparative example 1
2 groups of group, comparative example: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
Blank control group: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
Every component three parallel, each puts 50 tail prawns in parallel.
Method for breeding: it is surrounded in cultivating pool in the Penaeus Vannmei pond of raiser's raising with the nylon wire of 60 mesh
The mesh bag of 15m × 15m carries out the raising (each mesh bag corresponding in parallel) of test prawn in mesh bag, and Chinese medicinal aqua is uniform
Feeding is mixed to feed, when the feed that administration group is fed, every 1000 grams of feed addition Chinese medicinal aquas 1g.Blank control group feeds basic feeding
Material.It is respectively fed when every morning 9 and when afternoon 4 once, 5% feeding volume continuously feeds 5d.
(2) Hainan Province Wenchang City farm
Cultivate situation: pond size is between 2 mu -3 mu, and 0.8 meter -1.0 meters of the depth of water, discovery on June 6th, 2018 South America is white
Prawn liver shrimp body colour is partially yellow, and shrimp body colour unclarity, vigor is poor, and liver nigrescence is micro- swollen, and shrimp is just elongated tacky, chooses wherein two mouthfuls of ponds
The pool is as experiment pond.
Rearing conditions: dissolved oxygen in water is greater than 7mg/L, and pH value is 7.80 ± 0.50.
Example 1 group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, comparative example 1
2 groups of group, comparative example: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
Blank control group: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
Every component three parallel, each puts 50 tail prawns in parallel.
Method for breeding: it is surrounded in cultivating pool in the Penaeus Vannmei pond of raiser's raising with the nylon wire of 60 mesh
The mesh bag of 15m × 15m carries out the raising (each mesh bag corresponding in parallel) of test prawn in mesh bag, and Chinese medicinal aqua is uniform
Feeding is mixed to feed, when the feed that administration group is fed, every 1000 grams of feed addition Chinese medicinal aquas 1g.Blank control group feeds basic feeding
Material.It is respectively fed when every morning 9 and when afternoon 4 once, 5% feeding volume continuously feeds 5d.
(3) farm, Zhangzhou City, Fujian Province
Cultivate situation: pond size is between 2 mu -3 mu, and 0.8 meter -1.0 meters of the depth of water, the discovery South America on the 12nd of August in 2018
White shrimp liver shrimp body colour is partially yellow, and shrimp body colour unclarity, vigor is poor, liver atrophy, chooses wherein two mouthfuls of ponds and is used as experiment pond.
Rearing conditions: dissolved oxygen in water is greater than 7mg/L, and pH value is 7.80 ± 0.50.
Example 1 group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, comparative example 1
2 groups of group, comparative example: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
Blank control group: there is different degrees of exception in prawn specification 6.25-10g, the visible liver pancreas of appearance.
Every component three parallel, each puts 50 tail prawns in parallel.
Method for breeding: it is surrounded in cultivating pool in the Penaeus Vannmei pond of raiser's raising with the nylon wire of 60 mesh
The mesh bag of 15m × 15m carries out the raising (each mesh bag corresponding in parallel) of test prawn in mesh bag, and Chinese medicinal aqua is uniform
Feeding is mixed to feed, when the feed that administration group is fed, every 1000 grams of feed addition Chinese medicinal aquas 1g.Blank control group feeds basic feeding
Material.It is respectively fed when every morning 9 and when afternoon 4 once, 5% feeding volume continuously feeds 5d.
(4) farm, Guangxi province Qinzhou City
Cultivate situation: pond size is between 2 mu -3 mu, and 0.8 meter -1.0 meters of the depth of water, discovery on July 6th, 2018 South America is white
Prawn vigour of liver is poor, anorexia, and liver whitens micro- swollen, and shrimp is just elongated tacky, choose wherein two mouthfuls of ponds as experiment pond.
Rearing conditions: dissolved oxygen in water is greater than 7mg/L, and pH value is 7.80 ± 0.50.
Example 1 group, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, comparative example 1
2 groups of group, comparative example: there is different degrees of exception in prawn specification 6.25g-10g, the visible liver pancreas of appearance.
1 group of comparative example: prawn specification 6.25g-10g, there is different degrees of exception in the visible liver pancreas of appearance.
Blank control group: there is different degrees of exception in prawn specification 6.25-10g, the visible liver pancreas of appearance.
Every component three parallel, each puts 50 tail prawns in parallel.
Method for breeding: it is surrounded in cultivating pool in the Penaeus Vannmei pond of raiser's raising with the nylon wire of 60 mesh
The mesh bag of 15m × 15m carries out the raising (each mesh bag corresponding in parallel) of test prawn in mesh bag, and Chinese medicinal aqua is uniform
Feeding is mixed to feed, when the feed that administration group is fed, every 1000 grams of feed addition Chinese medicinal aquas 1g.Blank control group feeds basic feeding
Material.It is respectively fed when every morning 9 and when afternoon 4 once, 5% feeding volume continuously feeds 5d.
2, clinicing symptom observation
The South America prawn of above-mentioned 4 farms is to suffer from liver pancreas syndrome prawn, and the liver pancreas of the visible prawn of appearance has
(liver pancreas lighter or intensification are in green, black, yellow, red or color for different degrees of atrophy, enlargement, color exception
It is unequal) phenomena such as, find that hepatopancrease is frangible, nonelastic after dissection.Referring to left figure in Fig. 1.
After feeding embodiment Chinese medicinal aqua 5d, dissection discovery prawn liver uniform color arrives sepia, Shao Shuocheng in yellow
Existing rufous.Referring to Fig. 1 right figure.Feed the blank control group prawn liver rubicundity of basal feed, jaundice, greening, liver wither
Contracting, enlargement situation are serious.
3, clinical effectiveness determines
(1) criterion
Cure: aquiculture animal state is good, and ingestion ability is strong, and liver color is normally flexible, and it is different to find no liver
Often occur as.
Effective: aquiculture animal state is good, and ingestion ability is strong, and liver color is normally flexible, and accidental 1-2 tail has liver
Abnormal phenomenon.
Effective: aquiculture animal state is good, and ingestion ability is general, and liver color is normally flexible, with the presence of small part
There is liver anomalies phenomenon.
Invalid: symptom does not mitigate.
(2) determine result
1) Guangdong Province Mazhang District farm
2) Hainan Province Wenchang City farm
3) farm, Zhangzhou City, Fujian Province
4) farm, Guangxi province Qinzhou City
In addition, applicant faces 105 fish (yellow bone fish) liver pancreas syndromes from 2017 in Septembers, 2019
Bed observation treatment.Treatment method be using embodiment 1 Chinese medicinal aqua mix basal feed (30kg-40kg basal feed/
200mL), it feeds 2 times, continuously feeds 5 times daily.Effect determines referring to above-mentioned 3rd point " (1) criterion ".As a result: whole
On, clinical cure 86 (81.9%), effective 4 (3.8%) are effective 2 (1.9%), and it is invalid 13 (12.4%), it is efficient
It is 87.6%.Effect picture before and after use refers to Fig. 2.
To sum up, the present invention is by poplar flower, Chinese herbaceous peony and honeysuckle, Fructus Forsythiae, Radix Glycyrrhizae, Radix Astragali, dandelion, radix scutellariae, radix bupleuri, rheum officinale
Specific raw materials of traditional Chinese medicine composition formula is constituted together.The nourishing and protecting liver composition of the composition of raw materials can protect aquaculture dynamic
Object liver is injury-free or mitigates damage, avoids and causes Other diseases because of hepar damnification, reduces aquatic products on the whole and supports
Grow the generation of Animal diseases.Meanwhile using plant-derived composition of raw materials, the problem of environmental pollution and drug resistance is not present, poison is secondary
Act on it is small, safely and effectively, convenient for the use of modern high density, intensive culture mode.Meanwhile said preparation is for being damaged liver pancreas
With good repairing effect.It is in particular in: ensureing the cell activity of liver pancreas, it is ensured that juvenile prawn, which is smoothly spent, turns the liver phase；Increase
Strong liver pancreas improves body for the tolerance degree of adverse environment, reduces cultivated animals and answer for the capacity of decomposition of vivotoxin
Swash property damage and death；It ensures that cultivated animals store up blood ability, maintains the normality of body blood circulation；Ensure the normal occlusion of bile
At maintaining the integrality of the digestion and absorption function of enteron aisle, improve the immunocompetence of body.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of nourishing and protecting liver Chinese medicine composition, which is characterized in that the effective component of the Chinese medicine composition is by following weight
The raw material of part is prepared: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes, 20 parts of -80 parts of poplars
Flower, 20 parts of -60 parts of Chinese herbaceous peonies, 0 part of -60 parts of Radix Astragali, 0 part of -80 parts of dandelion, 0 part of -50 parts of radix scutellariae, 0 part of -60 parts of radix bupleuri and 0 part -
60 parts of rheum officinales.
2. nourishing and protecting liver Chinese medicine composition according to claim 1, which is characterized in that the Chinese medicine composition it is effective at
Dividing is prepared by the raw material of following parts by weight: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts of -80 parts of Radix Glycyrrhizaes,
45 parts of -75 parts of poplar flowers, 23 parts of -48 parts of Chinese herbaceous peonies, 14 parts of -48 parts of Radix Astragalis, 45 parts of -78 parts of dandelions, 20 parts of -45 parts of radix scutellariaes, 37
- 55 parts of radix bupleuri of part and 36 parts of -58 parts of rheum officinales.
3. nourishing and protecting liver Chinese medicine composition according to claim 1 or 2, which is characterized in that the Chinese medicine composition has
Imitating ingredient is prepared by the raw material of following parts by weight: 50 parts of -80 parts of honeysuckles, 60 parts of -100 parts of Fructus Forsythiaes, 50 parts -80 parts sweet
Grass, 45 parts of -60 parts of poplar flowers, 35 parts of -45 parts of Chinese herbaceous peonies, 30 parts of -48 parts of Radix Astragalis, 50 parts of -70 parts of dandelions, 25 parts of -36 parts of radix scutellariaes,
43 parts of -55 parts of radix bupleuri and 36 parts of -50 parts of rheum officinales.
4. the preparation method of the described in any item nourishing and protecting liver Chinese medicine compositions of claims 1 to 3, which is characterized in that the system
Preparation Method includes the following steps:
(1) raw material is taken, is added water to cook, filters, obtains that water mentions filtrate and water mentions filter residue；
(2) it takes the water to mention filter residue, adds alcohol solution dipping, diacolation obtains alcohol extracting filtrate；
(3) remove the ethyl alcohol in the alcohol extracting filtrate, by products therefrom and the water mention filtrate mix and to gained mixed liquor into
Row concentration.
5. the preparation method of nourishing and protecting liver Chinese medicine composition according to claim 4, which is characterized in that in step (1), institute
The weight for stating water is 8 times -18 times of weight of the raw material, and the number of the decoction is 1 time -2 times, each 2h-4h.
6. the preparation method of nourishing and protecting liver Chinese medicine composition according to claim 4, which is characterized in that in step (2), institute
Stating ethanol solution is the ethanol water that percent by volume is 50%-70%, and the weight of the ethanol solution is that the water proposes filter
4 times -8 times of the weight of slag, the time of the immersion are 40h-55h.
7. the preparation method of nourishing and protecting liver Chinese medicine composition according to claim 4, which is characterized in that in step (2), institute
The flow velocity for stating diacolation is 20ml/min-30ml/min.
8. according to the preparation method of the described in any item nourishing and protecting liver Chinese medicine compositions of claim 4 to 7, which is characterized in that step
Suddenly in (3), the mode of the concentration uses rotary evaporation, and the volume that products therefrom is concentrated is the 1/3-2/ of the mixeding liquid volume
3。
9. application of the described in any item compositions of claims 1 to 3 in the drug of preparation maintenance aquiculture animal liver.
10. application according to claim 9, which is characterized in that the drug is liquid medicine；Or/and the aquatic products is supported
Growing animal is shrimp, fish or crab.
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Application publication date: 20191122 |