CN103656505A - Fish saprolegnia resisting traditional Chinese medicine extractive water aqua and preparation method thereof - Google Patents
Fish saprolegnia resisting traditional Chinese medicine extractive water aqua and preparation method thereof Download PDFInfo
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Abstract
The invention discloses fish saprolegnia resisting traditional Chinese medicine extractive water aqua and a preparation method thereof. The traditional Chinese medicine extractive water aqua is prepared from traditional Chinese medicine extractives, a solvent, a cosolvent, a synergist, an emulsifying agent and water in proportions. The preparation method comprises the following steps: (1) respectively grinding dry traditional Chinese medicine materials by using an all-purpose grinding machine, putting traditional Chinese medicine powder in sealed plastic bags, and storing for later use; (2) putting the traditional Chinese medicine powder in an extraction kettle of a CO2 supercritical extraction instrument, collecting the traditional Chinese medicine extractives from a separation kettle I and a separation kettle II, and putting the traditional Chinese medicine extractives in a rotary concentration bottle; (3) putting the concentration bottle on a rotary evaporator, and collecting the traditional Chinese medicine extractives; and (4) mixing the traditional Chinese medicine extractives, adding the solvent according to the percentage content of the components of the water aqua so as to dissolve the traditional Chinese medicine extractives, respectively adding the cosolvent, the synergist and the emulsifying agent, mixing, and finally adding water so as to prepare the water aqua. The preparation method is practicable, and simple and convenient to operate. The fish saprolegnia resisting traditional Chinese medicine extractive water aqua is convenient to use, can resist fish saprolegnia, does not pollute environments after being used, and does not generate drug tolerance and drug residue in fish bodies.
Description
Technical field
The invention belongs to field of veterinary, more specifically relate to a kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation, also relate to a kind of preparation method of anti-Fish water mo(u)ld Chinese medicine extract water preparation, for preventing and treating saprolegniasis of aquatic animals simultaneously.
Background technology
Water mo(u)ld is a kind of filamentous fungi, mainly comprise Saprolegnia (
saprolegnia), Achyla (
achlya), silk capsule Pseudomonas (
aphanomyces) etc.Fish molds is widely distributed, is mainly present in freshwater, and to the subject range of temperature very wide (5 ~ 26 ℃), breeding thermophilic is 13 ~ 18 ℃.All can cause throughout the year each culture zone aquatic animal of the whole nation (as Cyprinidae and salmon fishes, perch, anguilla japonica and shrimp) to infect water mo(u)ld.Fish molds to host without strict selectivity, various aquatic animal, from ovum to each in age adult each Life Stages all can infect, in the winter time with easy infection more in early spring, and very easily there is infection the rapid spread of water mo(u)ld, cause a large amount of fishes dead, harm especially severe.
In order to suppress and kill the fish molds of water mo(u)ld control aquatic animal, use in a large number for a long time active drug is " malachite green oxalate " always.Although malachite green oxalate can better be killed water mo(u)ld, result of study shows, malachite green oxalate is residual for a long time in fish body, and can to mammal and the mankind, produce the effects such as teratogenesis, carcinogenic, mutagenesis by food chain; Formalin has pungent intense stimulus, and human body is also had to mutagenesis and the damaging effect such as carcinogenic.In addition, the life-time service of these chemical drugss also can cause the pollution of breeding environment.Therefore,, in view of the harm of traditional chemical medicine, some countries have forbidden using malachite green oxalate and formalin in edible fishes breeding process.In 2002 No. 192 bulletin of China Ministry of Agriculture, just listed in veterinary drug and other compound inventory > > of < < food animal forbidding.However, in actual aquaculture, because lacking, substitute the medicine that malachite green oxalate suppresses and kill water mo(u)ld, malachite green oxalate is still used in culture fishery in a large number, thereby cause fish quality problem, and then affect human health, be also unfavorable for that the health of culture fishery, sustainable development are fast.Therefore, develop antifungal drugs used in aquiculture efficient, low toxicity very necessary and urgent.
A kind of medicine and application thereof that prevents and treats Fish fish molds of Yang Xianle etc. (2013) application Chinese patent (CN103142592A), the medicine providing is selected from brominated industrial bactericide, pyrithione salt, iodo propinyl compound, the phenol analog derivative that contains compound structure (I), the compound that contains aldehyde radical, antifungus agent 10, any one or a few the combination of the two fen arsenic (OBPA) of 10 '-oxo, Radix Rehmanniae Preparata, Radix Sanguisorbae, Pericarpium Granati, Folium Isatidis, Herba Artemisiae Annuae; Grandson pari (2011) applies for a patent the method for (CN201110092891.4) a kind of methylene blue treatment fish body fish molds, a kind of method with methylene blue treatment fish body fish molds is provided, first the method is made a definite diagnosis Fish seed and is suffered from after fish molds, by methylene blue water dissolution, then press the full pond float of 2-4g/m3 water body final concentration, and can be used in conjunction 2 times depending on the state of an illness; Wang Jiqiao etc. (2005) Chinese patents (CN200510047346.8) disclose a kind of fish, shrimp, Eriocheir sinensis antifungal Chinese herbal and crude drugs preparations, it is characterized in that each weight percentages of components is: Galla Chinensis 40～70%, Cortex Phellodendri 10～30%, Cortex Moutan 10～30%, Herba Houttuyniae 10～30%, after decocting for the dipping of fish body, fish roe or stifling, it is bacteriostasis and sterilization, antiviral, parasite killing directly, do not produce drug resistance, and can regulate and improve immunity of organisms, strengthen the resistance against diseases of animal body.The medicine of the control fish molds described in previous patent, mainly contain compound recipe, the Chinese medicine preparation of chemicals, chemicals and Chinese medicine, wherein Chinese medicine preparation is the water decoction of the full medicine of Chinese medicine, but has no report about paper and the patent of the water preparation of the active Chinese drug component supercritical carbon dioxide extraction thing of water resistant mycete.Therefore, this patent is made water preparation by the activity extract with the water resistant mycete of supercritical carbon dioxide extraction from Chinese medicine, has considered on the one hand effectiveness and the safety of water resistant mycete, has considered on the other hand the convenience of using.
Summary of the invention
The present invention seeks to overcome prior art weak point, is to be to provide a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, and formula rationally, easy to use, easily grasp using dosage, selected Chinese herb price is relatively low, in liberal supply, easily obtain, can produce in batches; The effect of the anti-Fish water mo(u)ld of this water preparation is better, is 3-20 times of common Chinese water boiling and extraction thing.
Another object of the object of the invention is the preparation method that has been to provide a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, easy to implement the method, easy and simple to handle, this Chinese medicine extract preparation is easy to use, the anti-Fish water mo(u)ld of energy, after use, do not pollute the environment, can not produce drug resistance, can in fish body, not produce drug residue problem.
In order to realize above-mentioned object, the present invention adopts following technical measures:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, first adopts CO
2supercritical extraction instrument extracts the activity extract in preferred Chinese medicine, then by activity extract (one or more), through preferred dissolution with solvents, add emulsifying agent, cosolvent and synergist after dilute with water make the water preparation of Chinese medicine extract.
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material:
Raw materials quality mark %
Chinese medicine extract 1-25%
Solvent 5-20%
Cosolvent 2-15%
Synergist 2-10%
Emulsifying agent 5-20%
Water 10-85%
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material: (preferable range):
Raw materials quality mark %
Chinese medicine extract 5-25%
Solvent 8-20%
Cosolvent 2-12%
Synergist 2-8%
Emulsifying agent 10-20%
Water 15-73%
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material: (better scope):
Raw materials quality mark %
Chinese medicine extract 10-25%
Solvent 10-20%
Cosolvent 2-10%
Synergist 2-5%
Emulsifying agent 10-18%
Water 22-66%
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material: (optimum range):
Raw materials quality mark %
Chinese medicine extract 10-20%
Solvent 10-15%
Cosolvent 5-10%
Synergist 3-5%
Emulsifying agent 10-15%
Water 35-62%
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material: (optimum):
Raw materials quality mark %
Chinese medicine extract 20%
Solvent 20%
Cosolvent 5%
Synergist 5%
Emulsifying agent 15%
Water 35%
Described for extract the Chinese medicine of the Chinese medicine extract of anti-Fish water mo(u)ld be Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis wherein a kind of or two to 16 kinds mix arbitrarily.
The solvent of described dissolving Chinese medicine extract is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF.
Described cosolvent is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF.
Preferably, described synergist is a kind of or two to the four kinds of mixing arbitrarily in azone, lauryl alcohol sodium sulfate, sodium lignin sulfonate, alkylphenol polyoxyethylene third.
Described emulsifying agent is a kind of or two to the ten kinds of mixing arbitrarily in octyl phenol polyoxy ethylene 15 ethers (OP-15), polysorbas20, polysorbate60, Tween 80, span 20, span 40, sim alkylphenol polyoxyethylene 4 ethers (OP-4), sim alkylphenol polyoxyethylene 5 ethers (OP-5), laurilsulfate sodium, emulsifying agent 0201B.
Described anti-Fish water mo(u)ld Chinese medicine extract water preparation, the weight percentage of said preparation Chinese medicine extract is 1-25%, solvent 5-20%, cosolvent 2-15%, emulsifying agent 5-20%, synergist 2-10%, surplus is water.
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) by dry Chinese medicine, with omnipotent disintegrating machine, Chinese medicine material is pulverized respectively, Chinese medicine powder is placed and is chilled to room temperature (20-25 ℃), cross 30-50 mesh sieve, for extracting, if temporarily Chinese medicine powder need not be packed in sealing plastic bag, the refrigerator of putting into 4 ℃ saves backup.Described Chinese medicine is wherein a kind of or any two to the 16 kinds of mixing in Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis.
(2) Chinese medicine powder is put into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20-35 MPa, and extraction temperature is 30-50 ℃, separating still I pressure is 8-15 MPa, and the temperature of separating still I is 30-55 ℃, and the pressure of separating still II is 4-12 MPa, the temperature of separating still II is 25-40 ℃, CO
2flow velocity is 5-15 kg/h, entrainer is 75-95%(volume ratio) ethanol, the ratio of Chinese medicine powder and entrainer (quality and volume ratio) is 1:8-1:25, and extraction time is 2-5 h, from separating still I and separating still II, collects Chinese medicine extract in the concentrated bottle of rotation.
(3) will concentrate bottle and put on Rotary Evaporators, the bath temperature of Rotary Evaporators is made as 30-55 ℃, and vacuum is made as 0.6-1.0 MPa, and ethanol is volatilized, and collects Chinese medicine extract.
(4) a kind of Chinese medicine extract of or any two to 16 kinds of mixing is mixed, according to the composition percentage composition of this water preparation, first add preferred solvent (5-20%), limit edged shakes gently, Chinese medicine extract (1-25%) is dissolved, then add respectively preferred cosolvent (2-15%), synergist (2-10%) and emulsifying agent (5-20%), evenly mix, finally add water (10-85%) fully after stirring, can be made into water preparation.
Described solvent is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF;
Described cosolvent is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF;
Described synergist is a kind of or two to the four kinds of mixing arbitrarily in azone, lauryl alcohol sodium sulfate, sodium lignin sulfonate, alkylphenol polyoxyethylene third;
Described emulsifying agent is a kind of or two to the ten kinds of mixing arbitrarily in octyl phenol polyoxy ethylene 15 ethers (OP-15), polysorbas20, polysorbate60, Tween 80, span 20, span 40, sim alkylphenol polyoxyethylene 4 ethers (OP-4), sim alkylphenol polyoxyethylene 5 ethers (OP-5), laurilsulfate sodium, emulsifying agent 0201B.
The present invention compared with prior art, has the following advantages:
(1) the present invention extracts the active substance to the effective Chinese medicine of Fish water mo(u)ld to concentrate, and is more conducive to the killing action to water mo(u)ld;
(2) the present invention adopts CO
2supercritical extraction instrument, and use the ethanol of 75-95% second to extract the active substance in Chinese medicine as entrainer, use more separately ethanol to do the extract that extractant extracts more abundant, the extract that the method for boiling with independent employing decocting is extracted is compared more concentrated its effective content that improves, more be conducive to extraction volatile in Chinese medicine, unsettled active substance, and use CO
2the effect of the water preparation inhibition water mo(u)ld that supercritical Chinese medicine extract is made is better than the water boiling and extraction thing of Chinese medicine, in Table 1:
Table 1 Chinese medicine CO
2supercritical extract water preparation and Chinese medicine water boiling and extraction liquid 50% minimal inhibitory concentration (MIC
50) relatively
| Drug extract and preparation title | 24 h, MIC 50 (mg/L) |
| Galla Chinensis water boiling and extraction liquid | 300 |
| Rhizoma Et Radix Notopterygii water boiling and extraction liquid | 100 |
| Rhizoma Coptidis water boiling and extraction liquid | 250 |
| Rhizoma Polygoni Cuspidati water boiling and extraction liquid | 650 |
| Chinese medicine extract water preparation | 32(is with Chinese medicine abstract content meter) |
(3) anti-Fish Chinese medicine extract is made into the water preparation of extract, can be directly used in little water body fish body and fish roe are carried out disinfection, after also can being diluted, full pool spilling head is used, and this dosage form is also conducive to transportation and storage simultaneously.
The specific embodiment
Following examples are used for illustrating the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material:
Raw materials quality mark %
Chinese medicine extract 10%
Solvent 14%
Cosolvent 4%
Synergist 2%
Emulsifying agent 15%
Water 55%
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) dry Galla Chinensis is pulverized with omnipotent disintegrating machine, the gallnut extract of pulverizing is placed into after room temperature, crossed 30 or 35 or 40 or 45 or 50 mesh sieves, for extracting.
(2) taking 0.5 kg gallnut extract is put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20 or 25 or 30 or 35MPa, extraction temperature is 40 or 45 or 50 ℃, separating still I pressure is 8 or 10 or 15 MPa, the temperature of separating still I is 35 or 40 or 45 or 55 ℃, the pressure of separating still II is 4 or 8 or 12 MPa, and the temperature of separating still II is 25 or 30 or 35 or 40 ℃, CO
2flow velocity is 5 or 10 or 15 kg/h, the ethanol that entrainer is 80%, and the ratio of gallnut extract and entrainer (quality and volume ratio) is 1:10, the ethanol of 5 L 80% divides 2 times and adds in extraction kettle, each 2.5 L, CO
2after supercritical extraction instrument is stable, add 2.5 L entrainer ethanol, extraction carries out adding after 1 h the entrainer ethanol of 2.5 mL again, and total extraction time is 2 or 3 or 5 h, every 1 h, from separating still I and separating still II, collects Galla Chinensis extract in the concentrated bottle of rotation.
(3) will concentrate bottle and put on Rotary Evaporators, the bath temperature of Rotary Evaporators is made as 30 or 35 or 40 or 45 or 50 or 55 ℃, and vacuum is made as 0.6 or 0.8 or 1.0 MPa, and ethanol is volatilized, and collects gallic extract.
(4) get the gallic extract of 50 g, add 70 mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then add respectively 20 mL dimethyl sulfoxide, 10 mL azones, 75 mL octyl phenol polyoxy ethylene 15 ethers, evenly mix, finally add water to 500 mL, after fully stirring, can be made into water preparation.
An anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material:
| Embodiment | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| Chinese medicine extract | 10% | 10% | 10% | 10% | 15% | 15% | 15% | 15% | 20% | 20% | 20% | 20% | 20% |
| Solvent | 10% | 10% | 10% | 10% | 10% | 10% | 12% | 15% | 15% | 15% | 15% | 20% | 20% |
| Cosolvent | 2% | 4% | 4% | 5% | 5% | 5% | 5% | 5% | 5% | 5% | 5% | 5% | 10% |
| Synergist | 2% | 4% | 5% | 5% | 2% | 4% | 5% | 5% | 2% | 4% | 5% | 5% | 5% |
| Emulsifying agent | 8% | 10% | 12% | 15% | 10% | 10% | 10% | 15% | 10% | 10% | 15% | 15% | 15% |
| Water | 67% | 62% | 59% | 55% | 58% | 54% | 45% | 45% | 48% | 46% | 40% | 35% | 30% |
| Amount to | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% | 100% |
Its preparation process is identical with embodiment 1.
Embodiment 15:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) dry Cortex Meliae and Pericarpium Granati are pulverized with omnipotent disintegrating machine respectively, the Melia azedarach L. corium farinosum of pulverizing and Punica granatum L. corium farinosum are placed into after room temperature, crossed 30 or 40 or 45 mesh sieves, for extracting.
(2) taking 0.5 kg Melia azedarach L. corium farinosum is put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20 or 25 or 35 MPa, extraction temperature is 30 or 40 or 50 ℃, separating still I pressure is 8 or 10 or 12 MPa, the temperature of separating still I is 35 or 45 or 50 ℃, the pressure of separating still II is 4 or 6 or 8 or 10 MPa, and the temperature of separating still II is 25 or 35 or 40 ℃, CO
2flow velocity is 5 or 8 or 10 or 12 or 15 kg/h, the ethanol that entrainer is 80%, and the ratio of Melia azedarach L. corium farinosum and entrainer (quality and volume ratio) is 1:10, the ethanol of 5 L 80% divides 2 times and adds in extraction kettle, each 2.5 L, CO
2after supercritical extraction instrument is stable, add 2.5 L entrainer ethanol, extraction carries out adding after 1 h the entrainer ethanol of 2.5 mL again, and total extraction time is 2 or 3 or 4 or 5 h, every 1 h, from separating still I and separating still II, collects Cortex Meliae extract in the concentrated bottle of rotation; Extract as stated above the extract of Cortex Meliae.
(3) will concentrate bottle and put on Rotary Evaporators, the bath temperature of Rotary Evaporators is made as 35 or 40 or 45 or 50 or 55 ℃, and vacuum is made as 0.6 or 0.7 or 0.8 or 0.9 or 1.0 MPa, and ethanol is volatilized, and collects respectively the extract of Cortex Meliae and Pericarpium Granati.
(4) get the extract of 50 g Cortex Meliaees and the extract of 25 g Pericarpium Granatis, add 70 mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then add respectively 20 mL dimethyl sulfoxide, 10 mL azones, 75 mL octyl phenol polyoxy ethylene 15 ethers, evenly mix, finally add water to 500 mL, after fully stirring, can be made into water preparation.
Other step is identical with embodiment 1.
Embodiment 16:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) dry Galla Chinensis, Cortex Meliae, Pericarpium Granati and Rhizoma Coptidis will be pulverized respectively with omnipotent disintegrating machine, the gallnut extract of pulverizing, Melia azedarach L. corium farinosum, Punica granatum L. corium farinosum and Rhizoma Coptidis powder are placed into after room temperature, cross respectively 30 or 40 or 45 or 50 mesh sieves, respectively for extracting.
(2) taking 0.5 kg gallnut extract is put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 25 or 30 or 35MPa, extraction temperature is 30 or 40 or 45 or 50 ℃, separating still I pressure is 8 or 10 or 12 or 15 MPa, the temperature of separating still I is 40 or 45 or 50 or 55 ℃, the pressure of separating still II is 4 or 8 or 10 or 12 MPa, and the temperature of separating still II is 30 or 35 or 40 ℃, CO
2flow velocity is 5 or 10 or 12 or 15 kg/h, and entrainer is 80%(volume ratio) ethanol, the ratio of gallnut extract and entrainer (quality and volume ratio) is 1:10, the ethanol of 5 L 80% divides 2 times and adds in extraction kettle, each 2.5 L, CO
2after supercritical extraction instrument is stable, add 2.5 L entrainer ethanol, extraction carries out adding after 1 h the entrainer ethanol of 2.5 mL again, and total extraction time is 3 or 4 or 5 h, every 1 h, from separating still I and separating still II, collects Galla Chinensis extract in the concentrated bottle of rotation.Extract respectively as stated above the active substance in Cortex Meliae, Pericarpium Granati, Rhizoma Coptidis.
(3) will concentrate bottle puts on Rotary Evaporators, the bath temperature of Rotary Evaporators is made as 35 or 40 or 45 or 50 ℃, vacuum is made as 0.6 or 0.8 or 0.9 or 1.0 MPa, and ethanol is volatilized, and collects respectively the extract of Galla Chinensis, Cortex Meliae, Pericarpium Granati and Rhizoma Coptidis.
(4) get extract, 30 extracts of g Pericarpium Granati and the extract of Rhizoma Coptidis 10 g of the gallic extract of 20 g, 40 g Cortex Meliaees, add 100 mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then add respectively 20 mL dimethyl sulfoxide, 25 mL azones, 75 mL octyl phenol polyoxy ethylene 15 ethers, evenly mix, finally add water to 500 mL, after fully stirring, can be made into water preparation.
The water preparation physicochemical property of preparation is:
Outward appearance: transparent single-phase liquid;
Low-temperature stability: do not occur layering, separate out without Chinese medicine extract for 1 week under 4 ℃ of conditions.
Ageing stability: stable under room temperature.
Other step is identical with embodiment 1.
Embodiment 17:
The bacteriostatic test of Chinese medicine extract water preparation of the present invention to water mo(u)ld
Bacterium source: water mo(u)ld derives from Shanghai Ocean University's country's aquatic animal cause of disease storehouse;
Test method adopts agar dilution, with reference to Chen Shiqi, and Qiu Junqiang, Cao Haipeng, etc. the fungistatic effect research [J] of antifungus agent to water mo(u)ld. Agricultural University Of Southwest, 2011,24(4): 1568-1572 tests, the results are shown in Table 2.
The inhibition test result of table 2. different formulations Chinese medicine extract water preparation to water mo(u)ld
Other step is identical with embodiment 1.
Claims (7)
1. an anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made by the mass fraction of following raw material:
Parts by weight of raw materials
Chinese medicine extract 1-25%
Solvent 5-20%
Cosolvent 2-15%
Synergist 2-10%
Emulsifying agent 5-20%
Water 10-85%;
Described Chinese medicine extract is wherein a kind of or two to the 16 kinds of mixing arbitrarily in Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis;
The solvent of described dissolving Chinese medicine extract is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF;
Described cosolvent is a kind of or two to the seven kinds of mixing arbitrarily in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF;
Described synergist is a kind of or two to the four kinds of mixing arbitrarily in azone, lauryl alcohol sodium sulfate, sodium lignin sulfonate, alkylphenol polyoxyethylene third;
Described emulsifying agent is a kind of or two to the ten kinds of mixing arbitrarily in octyl phenol polyoxy ethylene 15 ethers, polysorbas20, polysorbate60, Tween 80, span 20, span 40, sim alkylphenol polyoxyethylene 4 ethers, sim alkylphenol polyoxyethylene 5 ethers, laurilsulfate sodium, emulsifying agent 0201B.
2. a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
Raw material %
Chinese medicine extract 5-25%
Solvent 8-20%
Cosolvent 2-12%
Synergist 2-8%
Emulsifying agent 10-20%
Water 15-73%.
3. a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
Raw material %
Chinese medicine extract 10-25%
Solvent 10-20%
Cosolvent 2-10%
Synergist 2-5%
Emulsifying agent 10-18%
Water 22-66%.
4. a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
Raw material %
Chinese medicine extract 10-20%
Solvent 10-15%
Cosolvent 5-10%
Synergist 3-5%
Emulsifying agent 10-15%
Water 35-62%.
5. a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
Raw material %
Chinese medicine extract 20%
Solvent 20%
Cosolvent 5%
Synergist 5%
Emulsifying agent 15%
Water 35%.
6. a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
Raw material %
Chinese medicine extract 10%
Solvent 14%
Cosolvent 4%
Synergist 2%
Emulsifying agent 15%
Water 55%.
7. the preparation method of a kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation claimed in claim 1, the steps include:
(1) by dry Chinese medicine, with omnipotent disintegrating machine, Chinese medicine material is pulverized respectively, Chinese medicine powder is placed and is chilled to room temperature, cross 30-50 mesh sieve, Chinese medicine powder is packed in sealing plastic bag, the refrigerator of putting into 4 ℃ saves backup, and described Chinese medicine is wherein a kind of or two to the 16 kinds of mixing arbitrarily in Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis;
(2) Chinese medicine powder is put into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20-35 MPa, and extraction temperature is 30-50 ℃, separating still I pressure is 8-15 MPa, and the temperature of separating still I is 30-55 ℃, and the pressure of separating still II is 4-12 MPa, the temperature of separating still II is 25-40 ℃, CO
2flow velocity is 5-15 kg/h, and entrainer is the ethanol of 75-95% volume ratio, and the ratio quality of Chinese medicine powder and entrainer and volume ratio are 1:8-1:25, and extraction time is 2-5 h, from separating still I and separating still II, collects Chinese medicine extract in the concentrated bottle of rotation;
(3) will concentrate bottle and put on Rotary Evaporators, the bath temperature of Rotary Evaporators is made as 30-55 ℃, and vacuum is made as 0.6-1.0 MPa, and ethanol is volatilized, and collects Chinese medicine extract;
(4) a kind of Chinese medicine extract of or any two to 16 kinds of mixing is mixed, composition percentage composition according to this water preparation first adds solvent, limit edged shakes, Chinese medicine extract is dissolved, then add respectively cosolvent, synergist and emulsifying agent, evenly mix, be made into water preparation after finally adding water fully to stir.
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| CN106337028A (en) * | 2015-07-17 | 2017-01-18 | 上海海洋大学 | Cleaning agent, and preparation method and application thereof |
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| CN109813820B (en) * | 2019-01-30 | 2022-02-18 | 中国水产科学研究院长江水产研究所 | Method for identifying metabolite of phoxim in different tissues of freshwater fish |
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