CN103656505B - A kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation - Google Patents
A kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation Download PDFInfo
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Abstract
The present invention discloses a kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation and preparation method, Chinese medicine extract, solvent, cosolvent, synergist, emulsifying agent, water are mixed with Chinese medicine extract water preparation in proportion, its step: (1) is by dry Chinese medicine, with omnipotent disintegrating machine, Chinese medicine material is pulverized respectively, Chinese medicine powder is loaded in sealing plastic bag, save backup; (2) Chinese medicine powder is put into CO
2in the extraction kettle of supercritical extraction instrument, collect Chinese medicine extract in spin concentration bottle from separating still I and separating still II; (3) concentrated bottle is put on Rotary Evaporators, collect Chinese medicine extract; (4) mixed by Chinese medicine extract, the composition of water preparation adds solvent, is dissolved by Chinese medicine extract, adds cosolvent, synergist and emulsifying agent respectively, and mixing, finally adds water and be made into water preparation.Easy to implement the method, easy and simple to handle, easy to use, the anti-Fish water mo(u)ld of energy, does not pollute the environment after using, can not produce drug resistance, can not produce drug residue problem in fish body.
Description
Technical field
The invention belongs to field of veterinary, more specifically relate to a kind of anti-Fish water mo(u)ld Chinese medicine extract water preparation, also relating to a kind of preparation method of anti-Fish water mo(u)ld Chinese medicine extract water preparation, for preventing and treating saprolegniasis of aquatic animals simultaneously.
Background technology
Water mo(u)ld is a kind of filamentous fungi, mainly comprises Saprolegnia (Saprolegnia), Achyla (Achlya), silk capsule Pseudomonas (Aphanomyces) etc.Fish molds is widely distributed, is mainly present in freshwater, and to the subject range very wide (5 ~ 26 DEG C) of temperature, breeding thermophilic is 13 ~ 18 DEG C.National each culture zone aquatic animal (as Cyprinidae and salmon fishes, perch, anguilla japonica and shrimp) all can be caused throughout the year to infect water mo(u)ld.Fish molds to host without strict selectivity, various aquatic animal, from ovum to each age adult each Life Stages all can infect, in the winter time with more easy infection in early spring, and very easily there is the infection of water mo(u)ld and rapid spread, cause a large amount of fish dead, harm especially severe.
In order to suppress and kill the fish molds of water mo(u)ld control aquatic animal, use active drug to be " malachite green oxalate " in a large number for a long time always.Although malachite green oxalate better can kill water mo(u)ld, result of study shows, malachite green oxalate remains for a long time in fish body, and produces the effects such as teratogenesis, carcinogenic, mutagenesis by food chain to mammal and the mankind; Formalin has pungent intense stimulus, and also has mutagenesis and the damaging effect such as carcinogenic to human body.In addition, the life-time service of these chemical drugss also can cause the pollution of breeding environment.Therefore, in view of the harm of traditional chemical medicine, some countries have forbidden using malachite green oxalate and formalin in edible fishes breeding process.The Ministry of Agriculture of China is just listed in No. 192 bulletin " veterinary drug of food animal forbidding and other compound inventory " for 2002.However, because lacking the medicine that alternative malachite green oxalate suppresses and kills water mo(u)ld in the aquaculture of reality, malachite green oxalate is still widely used in culture fishery, thus cause fish quality problem, and then affect human health, be also unfavorable for that the health of culture fishery, sustainable development are fast.Therefore, antifungal drugs used in aquiculture that is efficient, low toxicity is developed very necessary and urgent.
Yang Xianle etc. (2013) apply for Chinese patent (CN103142592A) a kind of medicine and application thereof preventing and treating Fish fish molds, the medicine provided is selected from brominated industrial bactericide, pyrithione salt, iodo propinyl compound, the phenol analog derivative containing compound structure (I), the compound containing aldehyde radical, antifungus agent 10, the two fen arsenic (OBPA) of 10 '-oxo, Radix Rehmanniae Preparata, Radix Sanguisorbae, Pericarpium Granati, Folium Isatidis, any one or a few combination of Herba Artemisiae Annuae; Grandson pari (2011) applies for a patent the method for (CN201110092891.4) a kind of methylene blue treatment fish body fish molds, provide the method for a kind of methylene blue treatment fish body fish molds, after first the method makes a definite diagnosis fish fry trouble fish molds, by methylene blue water dissolution, then press the full pond float of 2-4g/m3 water body final concentration, and 2 times can be used in conjunction depending on the state of an illness; Wang Jiqiao etc. (2005) Chinese patent (CN200510047346.8) discloses a kind of fish, shrimp, Eriocheir sinensis antifungal Chinese herbal and crude drugs preparations, it is characterized in that each weight percentages of components is: Galla Chinensis 40 ~ 70%, Cortex Phellodendri 10 ~ 30%, Cortex Moutan 10 ~ 30%, Herba Houttuyniae 10 ~ 30%, for the dipping of fish body, fish roe or stifling after decocting, it can directly bacteriostasis and sterilization, antiviral, parasite killing, do not produce drug resistance, and can regulate and improve immunity of organisms, strengthen the resistance against diseases of animal body.The medicine of the water prevention mildew described in previous patent, mainly contain the compound recipe of chemicals, chemicals and Chinese medicine, Chinese medicine preparation, wherein Chinese medicine preparation is the water decoction of the full medicine of Chinese medicine, but has no report about the paper of the water preparation of the active Chinese drug component supercritical carbon dioxide extraction thing of water resistant mycete and patent.Therefore, this patent makes water preparation by from Chinese medicine with the activity extract of the water resistant mycete of supercritical carbon dioxide extraction, considers effectiveness and the safety of water resistant mycete on the one hand, considers the convenience of use on the other hand.
Summary of the invention
The present invention seeks to overcome prior art weak point, be there are provided a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, formula is reasonable, easy to use, easily grasp using dosage, selected Chinese herb price is relatively low, in liberal supply, easily obtain, can produce in batches; The effect of the anti-Fish water mo(u)ld of this water preparation is better, is 3-20 times of common Chinese water boiling and extraction thing.
Another object of the object of the invention is the preparation method that there are provided a kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, easy to implement the method, easy and simple to handle, this Chinese medicine extract preparation is easy to use, the anti-Fish water mo(u)ld of energy, do not pollute the environment after using, can not drug resistance be produced, drug residue problem can not be produced in fish body.
In order to realize above-mentioned object, the present invention adopts following technical measures:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, first adopts CO
2supercritical extraction instrument extracts the activity extract in preferred Chinese medicine, then by activity extract (one or more), through preferred dissolution with solvents, add emulsifying agent, cosolvent and synergist after dilute with water make the water preparation of Chinese medicine extract.
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material:
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material: (preferable range):
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material: (better scope):
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material: (optimum range):
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material: (optimum):
The Chinese medicine of the described Chinese medicine extract for extracting anti-Fish water mo(u)ld is wherein a kind of or two to the ten six kinds of mixing arbitrarily in Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis.
The solvent of described dissolving Chinese medicine extract is one in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF or two to seven kinds of mixing arbitrarily.
Described cosolvent is one in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF or two to seven kinds of mixing arbitrarily.
Preferably, described synergist is one in azone, sodium laureth sulfate, sodium lignin sulfonate, alkylphenol polyoxyethylene third or two to four kinds of mixing arbitrarily.
Described emulsifying agent is one in octyl phenol polyoxyethylene 15 ether (OP-15), polysorbas20, polysorbate60, Tween 80, span 20, span 40, sim alkylphenol polyoxyethylene 4 ether (OP-4), sim alkylphenol polyoxyethylene 5 ether (OP-5), laurilsulfate sodium, emulsifying agent 0201B or two to ten kinds of mixing arbitrarily.
Described anti-Fish water mo(u)ld Chinese medicine extract water preparation, the weight percentage of said preparation Chinese medicine extract is 1-25%, solvent 5-20%, cosolvent 2-15%, emulsifying agent 5-20%, synergist 2-10%, and surplus is water.
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) by dry Chinese medicine, with omnipotent disintegrating machine, Chinese medicine material is pulverized respectively, Chinese medicine powder is placed and is chilled to room temperature (20-25 DEG C), cross 30-50 mesh sieve, for extraction, if temporarily Chinese medicine powder need not be loaded in sealing plastic bag, the refrigerator putting into 4 DEG C saves backup.Described Chinese medicine is one in Rhizoma Curcumae Longae, Cortex Cinnamomi, Pericarpium Granati, Herba Acalyphae, Rhizoma Acori Graminei, Folium Perillae, Folium Ricini, peppery Liao of water, Cortex Pseudolaricis, Galla Chinensis, Cortex Meliae, Rhizoma Et Radix Notopterygii, Fructus Toosendan, Rhizoma Polygoni Cuspidati, Fructus Litseae, Rhizoma Coptidis or any two to ten six kinds of mixing.
(2) Chinese medicine powder is put into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20-35MPa, and extraction temperature is 30-50 DEG C, separating still I pressure is 8-15MPa, and the temperature of separating still I is 30-55 DEG C, and the pressure of separating still II is 4-12MPa, the temperature of separating still II is 25-40 DEG C, CO
2flow velocity is 5-15kg/h, entrainer is the ethanol of 75-95% (volume ratio), the ratio (quality and volume ratio) of Chinese medicine powder and entrainer is 1:8-1:25, and extraction time is 2-5h, collects Chinese medicine extract in spin concentration bottle from separating still I and separating still II.
(3) put on Rotary Evaporators by concentrated bottle, the bath temperature of Rotary Evaporators is set to 30-55 DEG C, and vacuum is set to 0.6-1.0MPa, is volatilized by ethanol, collects Chinese medicine extract.
(4) by Chinese medicine extract mixing that is a kind of or any two to ten six kinds of mixing, preferred solvent (5-20%) is first added according to the composition percentage composition of this water preparation, limit edged shakes gently, Chinese medicine extract (1-25%) is dissolved, then preferred cosolvent (2-15%), synergist (2-10%) and emulsifying agent (5-20%) is added respectively, Homogeneous phase mixing, finally adds after water (10-85%) fully stirs and can be made into water preparation.
Described solvent is one in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF or two to seven kinds of mixing arbitrarily;
Described cosolvent is one in methanol, ethanol, acetone, n-butyl alcohol, isopropyl alcohol, dimethyl sulfoxide, DMF or two to seven kinds of mixing arbitrarily;
Described synergist is one in azone, sodium laureth sulfate, sodium lignin sulfonate, alkylphenol polyoxyethylene third or two to four kinds of mixing arbitrarily;
Described emulsifying agent is one in octyl phenol polyoxyethylene 15 ether (OP-15), polysorbas20, polysorbate60, Tween 80, span 20, span 40, sim alkylphenol polyoxyethylene 4 ether (OP-4), sim alkylphenol polyoxyethylene 5 ether (OP-5), laurilsulfate sodium, emulsifying agent 0201B or two to ten kinds of mixing arbitrarily.
The present invention compared with prior art, has the following advantages:
(1) the present invention is by extracting concentrated to the active substance of the effective Chinese medicine of Fish water mo(u)ld, is more conducive to the killing action to water mo(u)ld;
(2) the present invention adopts CO
2supercritical extraction instrument, and use the ethanol of 75-95% second to extract the active substance in Chinese medicine as entrainer, comparatively be used alone ethanol cook extractant extract extract more abundant, more easily concentrate compared with the extract adopting the method for soak by water to extract separately and improve its effective content, more be conducive to the extraction of active substance volatile, unstable in Chinese medicine, and use CO
2the water preparation that supercritical Chinese medicine extract is made suppresses the effect of water mo(u)ld to be better than the water boiling and extraction thing of Chinese medicine, in table 1:
Table 1 Chinese medicine CO
2supercritical extract water preparation and Chinese medicine water boiling and extraction liquid 50% minimal inhibitory concentration (MIC
50) compare
(3) anti-Fish Chinese medicine extract is made into the water preparation of extract, directly can be used in small size pond and carries out disinfection to fish body and fish roe, after also can being diluted, full pool spilling head uses, and this dosage form is also conducive to transport and storage simultaneously.
Detailed description of the invention
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1:
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) the omnipotent disintegrating machine of dry Galla Chinensis is pulverized, after the gallnut extract of pulverizing is placed into room temperature, crossed 30 or 35 or 40 or 45 or 50 mesh sieves, for extraction.
(2) take 0.5kg gallnut extract to be put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20 or 25 or 30 or 35MPa, extraction temperature is 40 or 45 or 50 DEG C, separating still I pressure is 8 or 10 or 15MPa, the temperature of separating still I is 35 or 40 or 45 or 55 DEG C, the pressure of separating still II is 4 or 8 or 12MPa, and the temperature of separating still II is 25 or 30 or 35 or 40 DEG C, CO
2flow velocity is 5 or 10 or 15kg/h, and entrainer is the ethanol of 80%, and the ethanol that the ratio (quality and volume ratio) of gallnut extract and entrainer is 1:10,5L80% divides 2 times to add in extraction kettle, each 2.5L, CO
2after supercritical extraction instrument is stable, add 2.5L entrainer ethanol, add the entrainer ethanol of 2.5mL after 1h is carried out in extraction again, total extraction time is 2 or 3 or 5h, collects Galla Chinensis extract every 1h in spin concentration bottle from separating still I and separating still II.
(3) put on Rotary Evaporators by concentrated bottle, the bath temperature of Rotary Evaporators is set to 30 or 35 or 40 or 45 or 50 or 55 DEG C, and vacuum is set to 0.6 or 0.8 or 1.0MPa, is volatilized by ethanol, collects gallic extract.
(4) the gallic extract of 50g is got, add 70mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then adds 20mL dimethyl sulfoxide respectively, 10mL azone, 75mL octyl phenol polyoxyethylene 15 ether, Homogeneous phase mixing, finally adds water to 500mL, can be made into water preparation after fully stirring.
A kind of anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the mass fraction of following raw material:
Its preparation process is identical with embodiment 1.
Embodiment 15:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) dry Cortex Meliae and Pericarpium Granati are pulverized with omnipotent disintegrating machine respectively, after the Melia azedarach L. corium farinosum of pulverizing and Punica granatum L. corium farinosum are placed into room temperature, crossed 30 or 40 or 45 mesh sieves, for extraction.
(2) take 0.5kg Melia azedarach L. corium farinosum to be put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20 or 25 or 35MPa, extraction temperature is 30 or 40 or 50 DEG C, separating still I pressure is 8 or 10 or 12MPa, the temperature of separating still I is 35 or 45 or 50 DEG C, the pressure of separating still II is 4 or 6 or 8 or 10MPa, and the temperature of separating still II is 25 or 35 or 40 DEG C, CO
2flow velocity is 5 or 8 or 10 or 12 or 15kg/h, and entrainer is the ethanol of 80%, and the ethanol that the ratio (quality and volume ratio) of Melia azedarach L. corium farinosum and entrainer is 1:10,5L80% divides 2 times to add in extraction kettle, each 2.5L, CO
2after supercritical extraction instrument is stable, add 2.5L entrainer ethanol, add the entrainer ethanol of 2.5mL after 1h is carried out in extraction again, total extraction time is 2 or 3 or 4 or 5h, collects Cortex Meliae extract every 1h in spin concentration bottle from separating still I and separating still II; Extract the extract of Cortex Meliae as stated above.
(3) put on Rotary Evaporators by concentrated bottle, the bath temperature of Rotary Evaporators is set to 35 or 40 or 45 or 50 or 55 DEG C, and vacuum is set to 0.6 or 0.7 or 0.8 or 0.9 or 1.0MPa, is volatilized by ethanol, collects the extract of Cortex Meliae and Pericarpium Granati respectively.
(4) extract of 50g Cortex Meliae and the extract of 25g Pericarpium Granati is got, add 70mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then adds 20mL dimethyl sulfoxide respectively, 10mL azone, 75mL octyl phenol polyoxyethylene 15 ether, Homogeneous phase mixing, finally adds water to 500mL, can be made into water preparation after fully stirring.
Other step is identical with embodiment 1.
Embodiment 16:
A preparation method for anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
(1) the omnipotent disintegrating machine of dry Galla Chinensis, Cortex Meliae, Pericarpium Granati and Rhizoma Coptidis will be pulverized respectively, after the gallnut extract of pulverizing, Melia azedarach L. corium farinosum, Punica granatum L. corium farinosum and Rhizoma Coptidis powder are placed into room temperature, cross 30 or 40 or 45 or 50 mesh sieves respectively, respectively for extraction.
(2) take 0.5kg gallnut extract to be put in into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 25 or 30 or 35MPa, extraction temperature is 30 or 40 or 45 or 50 DEG C, separating still I pressure is 8 or 10 or 12 or 15MPa, the temperature of separating still I is 40 or 45 or 50 or 55 DEG C, the pressure of separating still II is 4 or 8 or 10 or 12MPa, and the temperature of separating still II is 30 or 35 or 40 DEG C, CO
2flow velocity is 5 or 10 or 12 or 15kg/h, and entrainer is the ethanol of 80% (volume ratio), and the ethanol that the ratio (quality and volume ratio) of gallnut extract and entrainer is 1:10,5L80% divides 2 times to add in extraction kettle, each 2.5L, CO
2after supercritical extraction instrument is stable, add 2.5L entrainer ethanol, add the entrainer ethanol of 2.5mL after 1h is carried out in extraction again, total extraction time is 3 or 4 or 5h, collects Galla Chinensis extract every 1h in spin concentration bottle from separating still I and separating still II.Extract the active substance in Cortex Meliae, Pericarpium Granati, Rhizoma Coptidis as stated above respectively.
(3) concentrated bottle is put on Rotary Evaporators, the bath temperature of Rotary Evaporators is set to 35 or 40 or 45 or 50 DEG C, vacuum is set to 0.6 or 0.8 or 0.9 or 1.0MPa, is volatilized by ethanol, collects the extract of Galla Chinensis, Cortex Meliae, Pericarpium Granati and Rhizoma Coptidis respectively.
(4) the extract 10g of the gallic extract of 20g, the extract of 40g Cortex Meliae, the extract of 30g Pericarpium Granati and Rhizoma Coptidis is got, add 100mL ethanol, limit edged shakes gently, Galla Chinensis extract is dissolved, then adds 20mL dimethyl sulfoxide respectively, 25mL azone, 75mL octyl phenol polyoxyethylene 15 ether, Homogeneous phase mixing, finally adds water to 500mL, can be made into water preparation after fully stirring.
The water preparation physicochemical property of preparation is:
Outward appearance: transparent single-phase liquid;
Low-temperature stability: do not occur layering, separate out without Chinese medicine extract for 1 week under 4 DEG C of conditions.
Ageing stability: stable under room temperature.
Other step is identical with embodiment 1.
Embodiment 17:
Chinese medicine extract water preparation of the present invention is to the bacteriostatic test of water mo(u)ld
Bacterium source: water mo(u)ld derives from country of Shanghai Ocean University aquatic animal cause of disease storehouse;
Test method adopts agar dilution, with reference to Chen Shiqi, Qiu Junqiang, Cao Haipeng, etc. antifungus agent is studied [J] the fungistatic effect of water mo(u)ld. and Agricultural University Of Southwest, 2011,24 (4): 1568-1572 test, and the results are shown in Table 2.
Table 2. different formulations Chinese medicine extract water preparation is to the inhibition test result of water mo(u)ld
Other step is identical with embodiment 1.
Claims (6)
1. an anti-Fish water mo(u)ld active Chinese drug component extract water preparation, it is made up of the raw material of following mass percentage:
Described Chinese medicine is Galla Chinensis, Cortex Meliae, Pericarpium Granati and Rhizoma Coptidis;
The preparation method of described anti-Fish water mo(u)ld Chinese medicine extract water preparation, the steps include:
1) pulverized respectively by dry Chinese medicine material with omnipotent disintegrating machine, placed by Chinese medicine powder and be chilled to room temperature, cross 30-50 mesh sieve, loaded by Chinese medicine powder in sealing plastic bag, the refrigerator putting into 4 DEG C saves backup;
2) Chinese medicine powder is put into CO
2in the extraction kettle of supercritical extraction instrument, the pressure of extraction kettle is 20-35MPa, and extraction temperature is 30-50 DEG C, separating still I pressure is 8-15MPa, and the temperature of separating still I is 30-55 DEG C, and the pressure of separating still II is 4-12MPa, the temperature of separating still II is 25-40 DEG C, CO
2flow velocity is 5-15kg/h, and entrainer is the ethanol of 75-95% volume ratio, and the ratio of Chinese medicine powder and entrainer is with quality and volume basis for 1:8-1:25, and extraction time is 2-5h, collects Chinese medicine extract in spin concentration bottle from separating still I and separating still II;
3) put on Rotary Evaporators by concentrated bottle, the bath temperature of Rotary Evaporators is set to 30-55 DEG C, and vacuum is set to 0.6-1.0MPa, is volatilized by ethanol, collects Chinese medicine extract;
4) mixed by each Chinese medicine extract, first add ethanol according to the raw material percentage composition of this water preparation, limit edged shakes, Chinese medicine extract is dissolved, then add dimethyl sulfoxide, azone and octyl phenol polyoxyethylene 15 ether respectively, Homogeneous phase mixing, finally add after water fully stirs and be made into water preparation.
2. anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
3. anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
4. anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
5. anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
6. anti-Fish water mo(u)ld active Chinese drug component extract water preparation according to claim 1, is characterized in that:
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| CN109813820B (en) * | 2019-01-30 | 2022-02-18 | 中国水产科学研究院长江水产研究所 | Method for identifying metabolite of phoxim in different tissues of freshwater fish |
| CN111096970A (en) * | 2020-02-19 | 2020-05-05 | 上海海洋大学 | Chinese herbal medicine active ingredient composition with broad-spectrum anti-saprolegnia effect and preparation method thereof |
| CN112684055A (en) * | 2020-12-29 | 2021-04-20 | 江苏权正检验检测有限公司 | Method for determining veterinary drug residues through supercritical extraction |
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