CN102524271A - Cytisine and matrine insecticide water emulsion - Google Patents
Cytisine and matrine insecticide water emulsion Download PDFInfo
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- CN102524271A CN102524271A CN2011103845148A CN201110384514A CN102524271A CN 102524271 A CN102524271 A CN 102524271A CN 2011103845148 A CN2011103845148 A CN 2011103845148A CN 201110384514 A CN201110384514 A CN 201110384514A CN 102524271 A CN102524271 A CN 102524271A
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- China
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- matrine
- sparteine
- extract
- aqueous emulsion
- disinsection
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Abstract
The invention relates to cytisine and matrine insecticide water emulsion, which belongs to the water emulsion obtained through compounding 15 to 20 volume percent of gorse cytisine extract liquid with 8 to 10 volume percent of matrine extract liquid, wherein 1ml of medicine liquid of gorse cytisine extract liquid equals to 2g of crude drugs, and 1ml of medicine liquid of matrine extract liquid equals to 2g of crude drugs. The two kinds of plant source alkaloid of the cytisine and the matrine are mixed, so biopesticide with the advantages of high efficiency, low toxicity and little environment pollution is obtained. Major ingredients such as the cytosine and the matrine are natural products extracted from plants, and both the cytisine and the matrine have the insecticidal action, so strong killing effects are realized on pests, the drug resistance is not easy to occur, no harmful chemical pesticide ingredients are contained, the toxicity on people and livestock is low, no residue is left, and the environment is not polluted.
Description
Technical field
The invention belongs to biological pesticide technical field, particularly relate to a kind of sparteine matrine disinsection aqueous emulsion.
Background technology
In used the experience of medicinal plant in state-owned thousands of years; Unique traditional Chinese medical theory is arranged; The traditional Chinese herbal medicine compatibility theory is arranged, and botanical pesticide is wide in variety, and plant extractss such as thick grass, argy wormwood, black false hellebore, tobacco, Dalmatian chrysanthemum and trifoliate jewelvine use for many years as insecticide; Accumulated a lot of toxicitys, drug effect data, this provides rich material, theory and technical foundation for the development of new biopesticide.
Chinese Plants source pesticide source is very extensive, has found out that nearly 1300 various plants contain desinsection, Fungicidal active substance.From present result of study, agricultural chemicals mainly concentrates in the plants such as Meliaceae, composite family, pulse family, Celastraceae, Euphorbiaceae, Labiatae, Magnoliaceae, Aristolochiaceae, polygonaceae, Oleaceae, Liliaceae, Curcurbitaceae, sedge family, Cruciferae with plant.
Sparteine is the chemical composition in the yellow Central China of pulse family thermopsis fabaceae platymiscium lanceolata, and molecular formula is C
11H
14N
2O, structure is following.
The report that has only a small amount of sparteine insecticidal activity now has certain control efficiency to cabbage caterpillar, aphid, aleyrodid, diamond-back moth, leafhopper, plant hopper, twill leaf moth, beet webworm, mythimna separata, pine caterpillars, looper and low age cutworm etc.But because single agent quick-acting of botanical biological pesticide is relatively poor in producing, thereby exist the insect drug effect slowly, field efficacy is general, the spray medicine often, defective such as extension rate is lower.
Matrine is the chemical composition in the traditional Chinese medicine leguminous plant Sophora alopecuroide, belongs to the quinolizine pyridine class in the alkaloid, and molecular formula is C
15H
24N
2O, structure is following.
Verified at present: matrine has insecticidal action, and the mechanism of action makes the polypide protein coagulating then for paralysis insect nerve center, blocks the polypide pore, and insect is choked to death.The existing many reports of matrine control agricultural and sanitary insect pest have toxic action to various pests, like cabbage caterpillar, elm fleautiauxia armata black peach aphid, radish aphid, diamond-back moth, wheat aphid etc.
Sparteine and matrine are the quality raw materials compositions that can be used to make botanical biological pesticide.But because single agent quick-acting of botanical biological pesticide is relatively poor in producing, thereby exist the insect drug effect slowly, field efficacy is poor, the spray medicine often, defective such as extension rate is lower.
Do not see at present the complex preparation of sparteine and matrine.
Summary of the invention
The object of the invention just is to provide a kind of sparteine matrine disinsection aqueous emulsion.
The present invention realizes through following technical proposals:
A kind of sparteine matrine disinsection aqueous emulsion; It is characterized in that be by sparteine extract and the composite aqueous emulsion that forms of matrine extract wherein the consumption of sparteine extract be 15%～20%; Its per 1 milliliter of soup is equivalent to 2 gram crude drugs; The consumption of matrine extract is 8%～10%, and its 1 every milliliter of soup is equivalent to 2 gram crude drugs, and above-mentioned consumption is in percent by volume;
Above-mentioned sparteine extract is that rotary evaporation concentrates the back gained with suction filtration after the yellow China employing alcohol immersion extraction of the lanceolata after pulverizing or ultrasonic Extraction or the alcohol reflux heating and extracting;
Above-mentioned matrine extract is that rotary evaporation concentrates the back gained with suction filtration after the Sophora alopecuroide employing alcohol immersion extraction after pulverizing or ultrasonic Extraction or the alcohol reflux heating and extracting;
Described alcohol immersion is extracted and is meant that adopting 10～15 times, volume fraction is that 60%～90% ethanol soaked 12～36 hours at normal temperatures;
Described ultrasonic Extraction be meant adopt 10～15 times, volume fraction be behind 60%～90% the dissolve with ethanol again under 35～55 ℃ of temperature condition, ultrasonic Extraction 30～60min under the 70-100MHz;
Described alcohol reflux heating and extracting is meant that adopting 10～15 times, volume fraction is to extract 1.5 hours 50～70 ℃ of temperature condition refluxed behind 60%～90% the dissolve with ethanol again;
The auxiliary agent of above-mentioned aqueous emulsion is synergist, solvent and emulsifier;
Said synergist is castor oil and turpentine oil, and the castor-oil plant after wherein castor oil adopts and will pulverize is soaked at normal temperatures with the acetone of 10～15 times of amounts and separates 12～36 hours, and suction filtration, rotary evaporation are concentrated into every milliliter of soup and are equivalent to 2 and restrain crude drugs and obtain;
Said solvent is an acetone;
Said emulsifier is one or more among farming breast 500, alkylbenzene and the OP-10.
The present invention mixes sparteine and matrine two plant species source alkaloids, thereby obtains efficient, low toxicity and the little biopesticide of environmental pollution.Main components such as sparteine that the present invention is used and matrine all are the natural productss that from plant, extracts; Sparteine and matrine all have insecticidal action, therefore insect are had very strong killing action, are difficult for developing immunity to drugs; Do not contain any harmful chemical pesticide composition; To people and animals' low toxicity, do not have residual, free from environmental pollution.
Below be sparteine matrine disinsection aqueous emulsion virulence test of the present invention:
This test is carried out in matrimony vine research institute of Ningxia Academy of Agri-Forestry Sciences laboratory, and infusion process is adopted in test, measures the tag virulence of compound to aphid.Compound method according in the embodiment 4 is mixed with stock solution; According to the prerun result storing solution water gradient dilution is become 250,500,1000 during use; 2000 times soup, with micro-dropper with the soup drop of certain volume on the pronotary of aphid.It is to cultivate in 50%～75% the culturing room that examination worm behind the drop is transferred to (28 ± 2) ℃, relative moisture respectively.Every processing repeats for 3 times, and each dosage is handled 30 of examination worms.Observe the aphid mortality then, calculate the lethality that each is handled, the result sees table 1.
The insecticidal effect of the different extension rates of table 1
Sparteine matrine disinsection aqueous emulsion field trial:
Experimental field be located at matrimony vine research institute of Ningxia Academy of Agri-Forestry Sciences experimental field, No. 1, the peaceful Qi of life in 2 years, and experimental field management is strict, and water and fertilizer condition is good.Test is established 1000 times of dilutions of sparteine matrine disinsection aqueous emulsion and is handled.Take the dispenser of foliar spray method at 1 instar larvae initial stage of origination, evenly to squirt leave dual sides degree of being.Respectively before dispenser, 1d, 3d, 5d, 7d behind the medicine, 7d, 14d investigate 6 times altogether behind the secondary medicine.During investigation every tree from East, West, South, North, different azimuth choose 5 branches, investigate the insect population number of aphid on the long tender tip of every branch 30cm.
Result of the test shows that 1000 times of sparteine matrine disinsection aqueous emulsions of the present invention have the better prevention effect to the matrimony vine aphid, and the 3d preventive effect is the highest behind the medicine, and preventive effect reaches more than 90%.
Embodiment
Embodiment 1
The acquisition of 1 sparteine extract
At first the yellow China of lanceolata was pulverized 60 mesh sieves with cracker, and then the raw material powder was used alcohol immersion, soaking parameter is 90% for the ethanol volume fraction, 10 times of amount of ethanol, normal temperature, 24 hours.Suction filtration obtains after rotary evaporation is concentrated then, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The acquisition of 2 matrine extracts
At first Sophora alopecuroide is pulverized, crossed 60 mesh sieves, then the raw material powder being used 15 times, volume fraction is to extract 70 ℃ of temperature condition refluxed behind 70% the dissolve with ethanol to obtain in 1.5 hours again, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 16%, matrine extract 8%, castor oil 16%, turpentine oil 20%, acetone 30%, emulsifier 10%.
Wherein emulsifier adopts farming breast 500 (calcium dodecyl benzene sulfonates): the ratio preparation of alkylbenzene=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant after will pulverizing to soak at normal temperatures with the acetone of 15 times of amounts and separates 24 hours, and suction filtration, rotary evaporation are concentrated into every milliliter of soup and are equivalent to 2 and restrain crude drugs and obtain.
Embodiment 2
The acquisition of 1 sparteine extract
At first the yellow China of lanceolata was pulverized 60 mesh sieves with cracker; Then the raw material powder being used 15 times, volume fraction is 60% dissolve with ethanol; And then temperature is under 35 ℃ of conditions, 100MHz ultrasonic Extraction 30min, suction filtration then; Rotary evaporation obtains after concentrating, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The acquisition of 2 matrine extracts
At first Sophora alopecuroide is pulverized, crossed 60 mesh sieves, then the raw material powder is used alcohol immersion, soaking parameter is 60% for the ethanol volume fraction, 15 times of amount of ethanol, normal temperature, 36 hours.Suction filtration obtains after rotary evaporation is concentrated then, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Mix promptly according to the raw material of following percent by volume and to get.
Sparteine extract 20%, matrine extract 10%, castor oil 10%, turpentine oil 25%, acetone 25%, emulsifier 10%.
Wherein emulsifier adopts the ratio preparation of farming breast 500 (calcium dodecyl benzene sulfonate): OP-10 (APES)=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant after will pulverizing to soak at normal temperatures with the acetone of 15 times of amounts and separates 24 hours, and suction filtration, rotary evaporation are concentrated into every milliliter of soup and are equivalent to 2 and restrain crude drugs and obtain.
Embodiment 3
The acquisition of 1 sparteine extract
At first the yellow China of lanceolata was pulverized 60 mesh sieves with cracker, then the raw material powder being used 10 times, volume fraction is to extract 50 ℃ of temperature condition refluxed behind 90% the dissolve with ethanol to obtain in 3 hours again, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The acquisition of 2 matrine extracts
At first Sophora alopecuroide is pulverized, crossed 60 mesh sieves, it is to extract 70 ℃ of temperature condition refluxed behind 90% the dissolve with ethanol to obtain in 1 hour that the raw material powder uses 12 times, volume fraction again, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 15%, matrine extract 10%, castor oil 18%, turpentine oil 22%, acetone 27%, emulsifier 8%.
Wherein emulsifier adopts the ratio preparation of farming breast 500 (calcium dodecyl benzene sulfonate): OP-10 (APES)=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant after will pulverizing to soak at normal temperatures with the acetone of 10 times of amounts and separates 36 hours, and suction filtration, rotary evaporation are concentrated into every milliliter of soup and are equivalent to 2 and restrain crude drugs and obtain.
Embodiment 4
The acquisition of 1 sparteine extract
At first the yellow China of lanceolata was pulverized 60 mesh sieves with cracker, then the raw material powder being used 10 times, volume fraction is to extract 70 ℃ of temperature condition refluxed behind 90% the dissolve with ethanol to obtain in 1 hour again, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The acquisition of 2 matrine extracts
At first Sophora alopecuroide being pulverized, crossed 60 mesh sieves, is 80% dissolve with ethanol then with 12 times of raw material powder uses, volume fraction; And then temperature is under 55 ℃ of conditions, 70MHz ultrasonic Extraction 60min, suction filtration then; Rotary evaporation obtains after concentrating, and the per 1 milliliter of soup of this extract is equivalent to 2 gram crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 18%, matrine extract 9%, castor oil 13%, turpentine oil 20%, acetone 30%, emulsifier 10%.
Wherein emulsifier adopts farming breast 500 (calcium dodecyl benzene sulfonates): the ratio preparation of alkylbenzene=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant after will pulverizing to soak at normal temperatures with the acetone of 10 times of amounts and separates 36 hours, and suction filtration, rotary evaporation are concentrated into every milliliter of soup and are equivalent to 2 and restrain crude drugs and obtain.Embodiment of the present invention is not limited to the mode that the foregoing description provides.
Claims (11)
1. sparteine matrine disinsection aqueous emulsion; It is characterized in that it being by sparteine extract and the composite aqueous emulsion that forms of matrine extract; Wherein the consumption of sparteine extract is 15%～20%, and its per 1 milliliter of soup is equivalent to 2 gram crude drugs, and the consumption of matrine extract is 8%～10%; Its 1 every milliliter of soup is equivalent to 2 gram crude drugs, and above-mentioned consumption is in percent by volume.
2. according to the described sparteine matrine of claim 1 disinsection aqueous emulsion; It is characterized in that above-mentioned sparteine extract is that rotary evaporation concentrates the back gained with suction filtration after the yellow China employing alcohol immersion extraction of the lanceolata after pulverizing or ultrasonic Extraction or the alcohol reflux heating and extracting.
3. according to the described sparteine matrine of claim 1 disinsection aqueous emulsion; It is characterized in that above-mentioned matrine extract is that rotary evaporation concentrates the back gained with suction filtration after the Sophora alopecuroide employing alcohol immersion extraction after pulverizing or ultrasonic Extraction or the alcohol reflux heating and extracting.
4. according to claim 2 or 3 described sparteine matrine disinsection aqueous emulsions, it is characterized in that described alcohol immersion is extracted to be meant that adopting 10～15 times, volume fraction is that 60%～90% ethanol soaked 12～36 hours at normal temperatures.
5. according to claim 2 or 3 described sparteine matrine disinsection aqueous emulsions; It is characterized in that described ultrasonic Extraction be meant adopt 10～15 times, volume fraction be behind 60%～90% the dissolve with ethanol again under 35～55 ℃ of temperature condition, ultrasonic Extraction 30～60min under the 70-100MHz.
6. according to claim 2 or 3 described sparteine matrine disinsection aqueous emulsions, it is characterized in that described alcohol reflux heating and extracting is meant that adopting 10～15 times, volume fraction is to extract 1～3 hour 50～70 ℃ of temperature condition refluxed behind 60%～90% the dissolve with ethanol again.
7. according to the described sparteine matrine of claim 1 disinsection aqueous emulsion, the auxiliary agent that it is characterized in that above-mentioned aqueous emulsion is synergist, solvent and emulsifier.
8. according to the described sparteine matrine of claim 7 disinsection aqueous emulsion, it is characterized in that said synergist is castor oil and turpentine oil.
9. according to the described sparteine matrine of claim 7 disinsection aqueous emulsion, it is characterized in that said solvent is an acetone.
10. according to the described sparteine matrine of claim 7 disinsection aqueous emulsion, it is characterized in that said emulsifier is one or more among farming breast 500, alkylbenzene and the OP-10.
11. according to the described sparteine matrine of claim 8 disinsection aqueous emulsion; It is characterized in that said castor oil adopts castor-oil plant after will pulverizing to soak at normal temperatures with the acetone of 10～15 times of amounts and separates 12～36 hours; Suction filtration, rotary evaporation be concentrated into every milliliter of soup be equivalent to 2 the gram crude drugs obtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110384514.8A CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110384514.8A CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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| Publication Number | Publication Date |
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| CN102524271A true CN102524271A (en) | 2012-07-04 |
| CN102524271B CN102524271B (en) | 2014-12-10 |
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| CN201110384514.8A Expired - Fee Related CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896839A (en) * | 2017-10-19 | 2018-04-13 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of chrysomelid control method of illiciumverum |
| CN109006844A (en) * | 2018-08-24 | 2018-12-18 | 广西田园生化股份有限公司 | A kind of agricultural insecticide composition of the derivative containing sparteine and its utilization |
| CN112335697A (en) * | 2020-10-23 | 2021-02-09 | 湖南顺新龙辰生物工程有限公司 | Plant protective agent and preparation method thereof |
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| CN101199278A (en) * | 2006-12-14 | 2008-06-18 | 王国强 | Agricultural chemical composition containing sparteine |
| CN101624393A (en) * | 2009-08-04 | 2010-01-13 | 王答祺 | Method for extracting cytosine and thermopsine from thermopsis lanceolata seeds |
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2011
- 2011-11-28 CN CN201110384514.8A patent/CN102524271B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101199278A (en) * | 2006-12-14 | 2008-06-18 | 王国强 | Agricultural chemical composition containing sparteine |
| CN101624393A (en) * | 2009-08-04 | 2010-01-13 | 王答祺 | Method for extracting cytosine and thermopsine from thermopsis lanceolata seeds |
Non-Patent Citations (3)
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| 贾晓红 等: "苦豆籽中苦参碱的提取条件的优化选择", 《食品科技》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107896839A (en) * | 2017-10-19 | 2018-04-13 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of chrysomelid control method of illiciumverum |
| CN107896839B (en) * | 2017-10-19 | 2019-11-12 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of control method that illiciumverum is chrysomelid |
| CN109006844A (en) * | 2018-08-24 | 2018-12-18 | 广西田园生化股份有限公司 | A kind of agricultural insecticide composition of the derivative containing sparteine and its utilization |
| CN112335697A (en) * | 2020-10-23 | 2021-02-09 | 湖南顺新龙辰生物工程有限公司 | Plant protective agent and preparation method thereof |
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