CN101897337B - Microbial pesticide synergist and microbial pesticide containing same - Google Patents
Microbial pesticide synergist and microbial pesticide containing same Download PDFInfo
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- CN101897337B CN101897337B CN 201010236936 CN201010236936A CN101897337B CN 101897337 B CN101897337 B CN 101897337B CN 201010236936 CN201010236936 CN 201010236936 CN 201010236936 A CN201010236936 A CN 201010236936A CN 101897337 B CN101897337 B CN 101897337B
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- synergist
- microbial pesticide
- camptothecine
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- 239000000575 pesticide Substances 0.000 title claims abstract description 29
- 239000003175 pesticide synergist Substances 0.000 title claims abstract description 11
- VSJKWCGYPAHWDS-FQEVSTJZSA-N camptothecin Chemical group C1=CC=C2C=C(CN3C4=CC5=C(C3=O)COC(=O)[C@]5(O)CC)C4=NC2=C1 VSJKWCGYPAHWDS-FQEVSTJZSA-N 0.000 claims abstract description 53
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 12
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 12
- 241000701447 unidentified baculovirus Species 0.000 claims abstract description 7
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 239000000080 wetting agent Substances 0.000 claims description 14
- 239000000945 filler Substances 0.000 claims description 13
- 108700020497 Nucleopolyhedrovirus polyhedrin Proteins 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 5
- 239000005995 Aluminium silicate Substances 0.000 claims description 4
- 235000012211 aluminium silicate Nutrition 0.000 claims description 4
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 2
- 229960000892 attapulgite Drugs 0.000 claims description 2
- 239000004927 clay Substances 0.000 claims description 2
- 229910052570 clay Inorganic materials 0.000 claims description 2
- 229910052625 palygorskite Inorganic materials 0.000 claims description 2
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 2
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 2
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims description 2
- 229930182490 saponin Natural products 0.000 claims description 2
- 150000007949 saponins Chemical class 0.000 claims description 2
- 235000015099 wheat brans Nutrition 0.000 claims description 2
- 239000002917 insecticide Substances 0.000 abstract description 25
- 241000607479 Yersinia pestis Species 0.000 abstract description 8
- 229940127093 camptothecin Drugs 0.000 abstract description 4
- KLWPJMFMVPTNCC-UHFFFAOYSA-N Camptothecin Natural products CCC1(O)C(=O)OCC2=C1C=C3C4Nc5ccccc5C=C4CN3C2=O KLWPJMFMVPTNCC-UHFFFAOYSA-N 0.000 abstract description 3
- VSJKWCGYPAHWDS-UHFFFAOYSA-N dl-camptothecin Natural products C1=CC=C2C=C(CN3C4=CC5=C(C3=O)COC(=O)C5(O)CC)C4=NC2=C1 VSJKWCGYPAHWDS-UHFFFAOYSA-N 0.000 abstract description 3
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- 230000001276 controlling effect Effects 0.000 abstract 1
- 230000002147 killing effect Effects 0.000 abstract 1
- 244000144972 livestock Species 0.000 abstract 1
- 239000002075 main ingredient Substances 0.000 abstract 1
- 230000003405 preventing effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 16
- 230000002195 synergetic effect Effects 0.000 description 11
- 230000000749 insecticidal effect Effects 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 9
- 230000000853 biopesticidal effect Effects 0.000 description 7
- 239000000843 powder Substances 0.000 description 6
- 239000003905 agrochemical Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 231100000225 lethality Toxicity 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000759905 Camptotheca acuminata Species 0.000 description 3
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- 230000003449 preventive effect Effects 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
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- 101710151559 Crystal protein Proteins 0.000 description 1
- 241001412225 Firmiana simplex Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- LOUPRKONTZGTKE-WZBLMQSHSA-N Quinine Chemical compound C([C@H]([C@H](C1)C=C)C2)C[N@@]1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-WZBLMQSHSA-N 0.000 description 1
- RGDJCYRXKJVXKD-UHFFFAOYSA-N Saponin 3 Natural products COC(=O)C1(C)CCC2(CCC3(C)C(=CCC4C5(C)CCC(OC6OC(CO)C(O)C(O)C6OC7OC(CO)C(O)C(O)C7O)C(C)(C)C5CCC34C)C2C1)C(=O)O RGDJCYRXKJVXKD-UHFFFAOYSA-N 0.000 description 1
- 241000256247 Spodoptera exigua Species 0.000 description 1
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- 229930002341 quinoline alkaloid Natural products 0.000 description 1
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- 239000000375 suspending agent Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of biotic insecticides and relates to a microbial pesticide synergist. The main ingredient of the synergist is camptothecin or a derivative thereof. The invention relates to two microbial pesticides containing the synergist at the same time, namely a microbial pesticide containing bacillus thuringiensis and the camptothecin/camptothecin derivative, and a microbial pesticide containing baculovirus and the camptothecin/camptothecin derivative. The synergist can effectively improve the pest killing effect of the bacillus thuringiensis or baculovirus pesticide, enhance the pesticide effect and reduce the dosage of the microbial pesticide while ensuring the same pest preventing and controlling effect; moreover, the active ingredient camptothecin of the synergist is a plant-originated substance which is safe for people and livestock and natural enemies of the pests, can slow down the pesticide resistance of the pests to the microbial pesticide and is easy to degrade without residue; and the synergist is suitable to be used as an environmental-friendly microbial pesticide synergist and has great significance for popularization and application of the microbial pesticide.
Description
Technical field
The invention belongs to biological insecticides and application thereof, relate to a kind of microbial pesticide synergist and contain the biopesticide of this synergist.
Background technology
Biopesticide refers to the class pesticidal preparations that utilizes biological living or its metabolite that the pests such as insect, germ, weeds, nematode, muroid are prevented and treated; biopesticide comprises four classes such as microbial pesticide, botanical pesticide, animal pesticide and genetically modified organism agricultural chemicals; biopesticide has been compared following advantage with chemical pesticide: 1) selectivity is strong; safer to people and animals and various beneficial organism, protected natural enemy.2) little to eco-environmental impact, 3) can bring out insect epidemic disease etc.
Microorganism insecticide is the important part in biopesticide, being to have lethal effect or pathogenic microbe groups to make an insecticides with its metabolite or microorganism itself to host insect, is bacterium class (Bt), virus type, Mycophyta (white muscardine fungi, green muscardine fungus), entomopathogenic nematode class for what people were familiar with at present.And bacterium class and virus type are to use two kinds of wider microorganism insecticides in producing.In the bacterium class, with bacillus thuringiensis,Bt (Bacillus thuringiensis, be called for short Bt) be representative, it is a kind of microorganism insecticide that present output is maximum, range of application is the widest, but the shortcomings such as the preparation that usually uses Bt exists, and preventive effect is unstable, desinsection speed is slow, the longevity of residure is short, simultaneously also have the part insect to produce resistance to Bt, this is also the problem that the Bt insecticide faces; The baculovirus insecticides major defect is that desinsection speed is slow, needs just can make in 4～7 days the target pest of infection dead as baculoviral.Because the defective of these aspects makes microorganism insecticide be very limited in applying.
Under this background, seek synergistic matter as the synergistic component of microbial pesticide, improve the insecticidal toxicity of microbial pesticide, reduce insect density, the generation that reduces the pest damage degree and delay pest resistance to insecticide, and synergistic component is easily degraded at natural world, environmentally safe, be the principle of selective advance composition, synergistic matter uses separately without insecticidal activity or only has extremely low insecticidal activity, can significantly improve the insecticidal activity of preparation in the time of in adding microorganism insecticide to.Yet the at present research and development of microbial pesticide synergist is still the link of a weakness in the microorganism insecticide application.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of synergist that can effectively promote microbial pesticide insecticidal activity and safety and environmental protection is provided.
The present invention is achieved through the following technical solutions above-mentioned purpose:
Invention provides a kind of microbial pesticide synergist, take the camptothecine or derivatives thereof as main active ingredient.
Camptothecine (Camptothecin is called for short CPT) is a kind of pyrroles's quinoline alkaloid, and the chemical structural formula of camptothecine is:
Camptothecine derives from a kind of alkaloid that extracts in the fruit, leaf of Nyssaceae plant camptotheca acuminata (Camptotheca acuminata Decne).Camplotheca acuminata is the Nyssaceae plant, has another name called Camptotheca acuminata, thousand sheets tree, Nanjing Chinese parasol tree etc., and it is the distinctive plant of China, is distributed widely in the areas such as the Changjiang river, Chuan Nan, medically is being used for antineoplastic moral material.When using separately this camptothecine to be used for the control lepidopterous larvae, only have faint insecticidal action, but the present invention's discovery, camptothecine mixes use with microbial pesticide, the latter is had remarkable synergistic effect.Find in to a collection of alkaloidal screening test, when adding in microbial pesticide camptothecine or camptothecin derivative with the microbial pesticide coupling, its insecticidal effect is played obvious synergistic effect, can increase lethality and shorten the death time, can prevent that insect from continuing damage to crops, also can reduce the usage amount of microorganism insecticide simultaneously.
When using this microbial pesticide synergist, only need to add synergist in microbial pesticide, can be the derivative with camptothecine or camptothecine, with dry powder or water-soluble after add in microorganism insecticide, also can directly add in microorganism insecticide before dispenser.During application, the weight ratio of microbial pesticide and camptothecine/camptothecin derivative is 0.5～5: 10～40.
Except containing camptothecine, also contain wetting agent and filler in synergist; Wetting agent can be selected from one or more in fatty alcohol-polyoxyethylene ether, lauryl sodium sulfate, neopelex, Tea Saponin; Filler can be selected from one or several in diatomite, kaolin, wheat bran, potter's clay, calcium carbonate, attapulgite.And the mass fraction of camptothecine in synergist is 0.5～8.5%.
The present invention discloses the microbial pesticide of two kinds that contains this synergist.
First thuringiensis cladosporioides bacillus insecticide contains the camptothecine or derivatives thereof simultaneously in this agricultural chemicals.Wherein the weight ratio of camptothecine/camptothecin derivative and bacillus thuringiensis is 0.5～5: 10～40.Except this, can also contain above-mentioned wetting agent and filler in this agricultural chemicals.The mass fraction of each material is: bacillus thuringiensis dry powder 10～40%; Camptothecine/camptothecin derivative 0.5～5%; Wetting agent 3～10%; Filler 45～67.5%.
It two is baculovirus insecticides, contains simultaneously the camptothecine or derivatives thereof in this agricultural chemicals.Wherein the weight ratio of camptothecine/camptothecin derivative and baculoviral is 0.2～3: 5～30.Except this, can also contain above-mentioned wetting agent and filler in this agricultural chemicals.The mass fraction of each material is: baculovirus polyhedrin body 5～30%; Camptothecine/camptothecin derivative 0.1～3%; Wetting agent 3～10%; Filler 57～91.8%.
Invention shows through a large amount of tests, after the insecticidal crystal protein of camptothecine and Bt or baculovirus polyhedrin body are mixed, synergistic effect to two kinds of microorganism insecticides is remarkable, and can extend the longevity of residure of Bt, with the lethal time of shortening baculoviral to insect, prevent that insect from continuing damage to crops, reduce the usage amount of microorganism insecticide under equal preventive effect.
Compared with prior art, the present invention has following characteristics:
1. synergist provided by the present invention can promote the insecticidal effect of microorganism insecticide effectively, improves drug effect, can reduce the consumption of microorganism insecticide under equal preventive effect.
2. the present invention is that the synergist camptothecine that provides is also a kind of botanical components, during as the synergistic component of biopesticide, and easily degraded, noresidue, the biopesticide synergist that is suitable as a kind of environmental protection is promoted the use of.
3. synergist used in the present invention can delay insect to the drug-fast generation of the microorganism insecticides such as Bt, and is significant for the use of applying microorganism insecticide, the medication of minimizing high residue chemistry.
4. Bt or the baculovirus insecticides insecticidal toxicity that contains camptothecine provided by the present invention is higher 2～3 times than its independent use.
5. this invention preparation method is simple, raw material sources are extensive, with low cost.
Embodiment
In order to understand better essence of the present invention, the below is with the technology contents of example in detail invention, but the content of invention is not limited to this.
Embodiment 1: camptothecine is mass ratio in synergist:
Camptothecine: 3%
Wetting agent: neopelex 10%
Filler: diatomite 87%
Embodiment 2: the mass ratio that contains camptothecine and be each component in the Bt wetting powder of synergistic component:
Bacillus thuringiensis,Bt dry powder: 20%
Camptothecine: 0.3%
Neopelex: 5%
Kaolin is mended to 100%
Embodiment 3: the mass ratio of each component that contains camptothecine and be the bacillus thuringiensis suspending agent of synergistic component:
Bacillus thuringiensis,Bt dry powder: 20%
Camptothecine: 0.3%
Tea Saponin 3%
Calcium carbonate 76.7%
Embodiment 4: containing camptothecine is the preparation weight ratio of the baculovirus insecticides wetting powder of synergistic component:
Baculovirus polyhedrin body 10%,
Camptothecine 0.2%,
Wetting agent neopelex 5%,
Kaolin is mended to 100%
Embodiment 5
Take Bt standard items (15000IU/mg) sample 5g, camptothecine 0.6g mixes, use this formula with 2000 times of liquid of its dilution, 72 hours lethality to the cabbage loopers of 3 age ends reach 100%, and use separately Bt to only have 30%, use separately camptothecine lethality＜5%.
Embodiment 6:
Take Bt standard items (15000IU/mg) sample 5g, camptothecine 0.6g mixes, 2000 times of liquid of dilute with water are used for the control beet armyworm in 1～2 age, and control efficiency reached more than 85% in 72 hours, and be 40% for independent use Bt, using separately camptothecine is 20%.
Embodiment 7:
Take Bt standard items (15000IU/mg) sample 5g, camptothecine 0.2g mixes, 4000 times of liquid of dilute with water are used for the control diamond-back moth in 3 ages, and control efficiency reached more than 80% in 24 hours, and be 40% for independent use Bt, using separately camptothecine is 20%.
Embodiment 8:
Get that to contain the baculovirus polyhedrin bulk concentration be 10
4ACMNPV, adding wherein final concentration is the camptothecine of 0.06g/L, is used for the control cabbage looper in 4 ages, control efficiency reached 95.8% in 144 hours, and used separately 10
4The ACMNPV control efficiency be 37.5%, use separately camptothecine lethality 21%.
Claims (7)
1. a microbial pesticide synergist, is characterized in that synergist is take the camptothecine or derivatives thereof as main active ingredient; The mass fraction of described camptothecine or derivatives thereof in synergist is 0.5～8.5%.
2. microbial pesticide synergist as claimed in claim 1, is characterized in that described synergist also contains wetting agent and filler; Described wetting agent is one or more in fatty alcohol-polyoxyethylene ether, lauryl sodium sulfate, neopelex, Tea Saponin; Described filler is one or several in diatomite, kaolin, wheat bran, potter's clay, calcium carbonate, attapulgite.
3. the application of a microbial pesticide synergist as claimed in claim 1 is characterized in that adding the camptothecine or derivatives thereof in microbial pesticide, and wherein the weight ratio of microbial pesticide and camptothecine/camptothecin derivative is 5～40: 0.1～5.
4. a microbial pesticide that contains synergist, is characterized in that containing bacillus thuringiensis, contains simultaneously the camptothecine or derivatives thereof; The weight ratio of camptothecine/camptothecin derivative and bacillus thuringiensis is 0.5～5: 10～40.
5. microbial pesticide as claimed in claim 4, is characterized in that also containing wetting agent and filler, and the mass fraction of each material is: bacillus thuringiensis 10～40%; Camptothecine/camptothecin derivative 0.5～5%; Wetting agent 3～10%; Filler 45～86.5%.
6. a microbial pesticide that contains synergist, is characterized in that containing wild-type baculovirus or recombinant type baculovirus polyhedrin body or their combination, contains simultaneously the camptothecine or derivatives thereof; The weight ratio of camptothecine/camptothecin derivative and baculovirus polyhedrin body is 0.2～3: 5～30.
7. microbial pesticide as claimed in claim 6, is characterized in that also containing wetting agent and filler, and the mass fraction of each material is: baculovirus polyhedrin body 5～30%; Camptothecine/camptothecin derivative 0.2～3%; Wetting agent 3～10%; Other filler 57～91.8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010236936 CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010236936 CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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| Publication Number | Publication Date |
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| CN101897337A CN101897337A (en) | 2010-12-01 |
| CN101897337B true CN101897337B (en) | 2013-05-22 |
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| CN 201010236936 Expired - Fee Related CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103275095B (en) * | 2013-05-08 | 2015-07-22 | 中国农业科学院植物保护研究所 | 10-hydroxycamptothecine derivatives and applications thereof |
| CN109511680B (en) * | 2018-11-27 | 2021-12-07 | 上海沪联生物药业（夏邑）股份有限公司 | Biopesticide containing rice residue oil and fatty acid glyceride and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883267A (en) * | 2006-05-31 | 2006-12-27 | 浙江林学院 | Agricultural bactericide containing camptothecin or camptothecin derivative and application thereof |
| CN101299919A (en) * | 2005-11-01 | 2008-11-05 | 美国陶氏益农公司 | Pesticidally active compositions having enhanced activity |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101299919A (en) * | 2005-11-01 | 2008-11-05 | 美国陶氏益农公司 | Pesticidally active compositions having enhanced activity |
| CN1883267A (en) * | 2006-05-31 | 2006-12-27 | 浙江林学院 | Agricultural bactericide containing camptothecin or camptothecin derivative and application thereof |
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