CN102027958A - Insecticidal composition consisting of spinosad and fenobucarb - Google Patents

Abstract

The invention relates to an insecticidal composition consisting of spinosad and fenobucarb. The insecticidal composition comprises 0.5 to 10 percent of spinosad and 1 to 35 percent of fenobucarb. The insecticidal composition has good prevention and control effects on plutella xylostella, reduces using amount, delays medicament resistance, reduces pollution to the environment and does not cause chemical injury easily.

Description

The Pesticidal combination of pleocidin and Bassa

Technical field

The invention belongs to pesticide field, relate in particular to the Pesticidal combination of a kind of pleocidin and Bassa.

Background technology

It is a kind of macrolides Nuisanceless high-efficient biological insecticides that extract in the sugared many born of the same parents fermented liquid of thorn that pleocidin has another name called multiple killing teichomycin (Spinosad).Produce parent strain soil actinomycetes thorniness saccharopolyspora strain (the Saccharopolyspora spinosa Metrz ﹠amp of pleocidin; Yao) initial separation is from one of the Caribbean discarded wine brewing field.The mode of action novelty of pleocidin can sustained activation target insect acetylcholine nicotine receptor, but its binding site is different from nicotine and Imidacloprid.Pleocidin also can influence the GABA acceptor, but mechanism of action is unclear.Whether also do not know at present has cross tolerance with the insecticide of other types.These compounds can cause that target plant-feed insect such as caterpillar, leaf miner, thrips and leaf-feeding beetle are dead rapidly, although administrative department's strong request is not used when resistance occurs, the moderate residual activity of this compound has reduced resistance and the living possibility of mass-sending.The acting body of nicotinic acid acetylcholinergic receptor can sustained activation target insect acetylcholine nicotine receptor, but its binding site is different from nicotine and Imidacloprid.Pleocidin also can influence the GABA acceptor, insect is had tag fast and stomach poison function, and blade is had stronger osmosis, can kill subepidermal insect, and the longevity of residure is longer, and some insects are had certain ovicidal action.No systemic action.Can effectively preventing Lepidoptera, diptera and thrips insect, can prevent and treat well also that some measures the pest species of food blade greatly in coleoptera and the orthoptera, relatively poor to the control efficiency of sucking pest and mite class.Safer to the predator insect, unique because of insecticidal action mechanism, do not find as yet at present to have mutual drug-fast report with other insecticides.Plant safety there is not poisoning.Be suitable for using on vegetables, fruit tree, gardening, the crops.Can make insect benumb rapidly, paralyse, cause death at last.Its desinsection speed can compare favourably with chemical pesticide.Safe, and do not have the biological insecticides that cross resistance is low toxicity, efficient, low-residual with present common insecticide, existing insecticidal properties efficiently has again the characteristic of beneficial insect and mammalian safe being arranged, the most suitable pollution-free vegetable, fruit production application.Pleocidin, its structural formula is as follows:

Bassa (Fewobucarb), carbamate insecticide is mainly blocked normal nerve conduction by acetylcholine esterase inhibition, causes the imbalance of whole physiological and biochemical procedure, and insect is poisoned to death.Plant hopper, spider mite kind are had special efficacy, and desinsection is rapid, but residual effect is short, can only keep 4-5 days.Carbamates combines with cholinesterase so that compound molecule is whole, and inhibitory action reduces after the hydrolysis, so virulence is lower, metabolism faster is an innocuous substance in animal and plant body and soil.Simultaneously such material has also that quick-acting is good, the lasting period short, selectivity is strong and with other kind pesticide compositionals the characteristics of notable synergistic effect is arranged.

Bassa (Fewobucarb) chemical name 2-isopropyl phenyl-N-methyl carbamate, its chemical constitution is as follows:

Because agricultural pests causes very big loss to crops production every year, consider the agricultural chemicals of long-term use single variety, impel insect to produce resistance easily, environment is caused bigger pollution, field trial shows, the existing medicament another kind of mixed pesticide different with mechanism of action, can not only postpone resistance and produce, can also improve drug effect and residual period, reduce usage amount, reduce cost, so we enlarge insecticidal spectrum with pleocidin and fenobucarb composite.Not only can effectively prevent and treat most of insect, reduce environmental pollution, also improve drug effect and lasting period.

Summary of the invention

The purpose of this invention is to provide that a kind of insecticidal activity is efficient, lasting effect is long, the Pesticidal combination of the high and low poison of quick-acting, environmental protection.Be specifically related to the pesticidal preparations that pleocidin and Bassa are basis.

Above-mentioned Pesticidal combination contains pleocidin 0.5%～10%, Bassa 1%～35%.

Utilize the described insecticides of the invention described above, be equipped with the known auxiliary agent of those skilled in that art, can make formulation of the present invention with those skilled in that art's known method.Its formulation that can process is suspending agent, wetting powder, water dispersible granules.

Advantage that the present invention has and beneficial effect: 1. the desinsection scope is wide; 2. the lasting period is long; 3. low toxicity, noresidue, nuisanceless, environmentally safe; 4. use compound formulation, two kinds of insecticides also have synergistic effect, and the consumption of two kinds of insecticides reduces during all than single use, thereby has improved insecticidal effect, has increased safety, has reduced environmental pollution.

Embodiment

Following instantiation is in order to further describing the present invention, but the present invention absolutely not only only limits to following these embodiment.Percentage all is weight percentage in the prescription, and employed former medicine and auxiliary agent are other producers and buy.

Formulation examples is as follows:

1, the preparation of suspending agent

According to the prescription requirement, be medium with water, will grind in the adding sand mills such as active ingredient, wetting agent, dispersant, thickener, defoamer, the back adds emulsification in the high-shear emulsifying still, makes suspending agent.

Prescription one (26% suspending agent)

Component weight percent content

Pleocidin 1%

Bassa 25%

Lauryl sodium sulfate 6%

Sodium lignin sulfonate 4%

Carboxymethyl cellulose 0.2%

Xanthans 0.2%

Defoamer 0.15%

Water supplies 100%

Prescription two (8% suspending agents)

Component weight percent content

Pleocidin 2.5%

Bassa 5.5%

Dodecyl sodium sulfate 3%

Sodium lignin sulfonate 4%

Carboxymethyl cellulose 0.2%

Aluminium-magnesium silicate 0.1%

Defoamer 0.1%

Water supplies 100%

2, the preparation of wetting powder

Active ingredient, wetting agent, dispersant, filler etc. are fully mixed in the ratio of prescription, and after ultrafine crusher was pulverized, fineness reached 98% by 325 mesh standard sieves, promptly gets wetting powder.

Prescription three (10% wetting powders)

Component weight percent content

Pleocidin 5%

Bassa 5%

Dodecyl sodium sulfate 3%

Polyvinylpyrrolidone 4%

Carboxymethyl cellulose 0.2%

Ammonium sulfate 10%

Sodium bentonite complements to 100%

3, the preparation of water dispersible granules

Active ingredient, wetting agent, dispersant, adhesive, filler etc. are fully mixed in the ratio of prescription, after ultrafine crusher is pulverized, add an amount of purified water, make water dispersible granules through kneading, granulation, drying, screening.

Prescription four (17.5% water dispersible granules)

Component weight percent content

Pleocidin 7.5%

Bassa 10%

Lauryl sodium sulfate 3%

Polyvinylpyrrolidone 5%

Carboxymethyl cellulose 0.2%

Draw back powder 0.3%

Ammonium sulfate 8%

Kaolin complements to 100%

Prescription five (20% water dispersible granules)

Component weight percent content

Pleocidin 5%

Bassa 15%

Dodecyl sodium sulfate 3%

Polyvinylpyrrolidone 6%

Carboxymethyl cellulose 0.2%

Aluminium-magnesium silicate 0.5%

Polyvinyl alcohol 0.2%

Kaolin surplus 1/2

Sodium bentonite surplus 1/2

Diamond-back moth is the primary pest on the brassicaceous vegetable, calls the square moth, hangs corpse worm, little blue or green worm etc.Adult is small-sized moth class; Larva is active, meet when frightened twisting retreat or weave silk sagging, mature larva body yellow green, a taupe, the polypide middle part is thick, two ends are tiny of the shuttle shape.Each big vegetable-growing area takes place throughout the year, takes place particularly serious in 3～June, 9～December.Adult is many to lay eggs on bud, tender leaf and spray.After the larva hatching just leaf mining get food mesophyll, stay epidermis, on the dish leaf, form transparent one by one spot, full leaf is eaten and is reticulated when serious; Just be hidden in blade back harm after 2 ages, cause the dish leaf to incise; Larva also can endanger tender stem, young pod and seed on the dish of reserving seed for planting, influence solid.In recent years, agricultural chemicals uses in a large number, and the pesticide resistance of diamond-back moth sharply strengthens, and causes increasing agricultural chemicals that diamond-back moth was lost efficacy.

The method of indoor biometrics is: adopt capillary trace drop method, capillary trace dropper volume is 1.0 μ L.With micro-dropper soup is pursued a drop in the back side of diamond-back moth 6 instar larvaes, each concentration is handled about 30 larva, the culture dish that it is 9cm that per 5 larvas are put into a diameter, the built-in a small amount of feed of ware is for getting food, in addition with 30 larvas of acetone drop in contrast.Larva after treatment still is placed in the receptacle, checks lethality behind the 48h.Data are handled with the SPSS software statistics.Obtain toxicity regression formula, the lethal concentration of 50, correlation coefficient etc., and obtain co-toxicity coefficient with the abundant method of Sun Yun.

The co-toxicity of mixture preparation adopts the abundant co-toxicity coefficient method representation of Sun Yun:

The theoretical toxicity index A=∑ of mixture preparation (toxicity index of certain medicine * percentage of this pharmaceutically active ingredient in mixture).

The co-toxicity coefficient C=of mixture preparation

* 100

C was synergistic effect greater than 120 o'clock, less than 80 o'clock be antagonism, be addition during 80-120.

The different proportionings of table 1 are to the toxicity test result of diamond-back moth

Table 2 pleocidin is tested the test of giving birth to of diamond-back moth

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	45	45	45
10	100	68	68	68
					20	100	74	74	74
40	100	92	92	92
					50	100	95	95	95
CK	100	0	?	?

Table 3 Bassa is tested the test of giving birth to of diamond-back moth

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	43	43	43
10	100	56	56	56
					20	100	79	79	79
40	100	91	91	91
					50	100	93	93	93
CK	100	0	?	?

The test of giving birth to of a pair of diamond-back moth of table 4 prescription is tested

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	36	36	36
10	100	48	48	48
					20	100	61	61	61
40	100	79	79	79
					50	100	85	85	85
CK	100	0	?	?

The test of giving birth to of two pairs of diamond-back moths of table 5 prescription is tested

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	37	37	37
10	100	51	51	51
					20	100	63	63	63
40	100	80	80	80
					50	100	87	87	87
CK	100	0	?	?

The test of giving birth to of three pairs of diamond-back moths of table 6 prescription is tested

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	41	41	41
10	100	47	47	47
					20	100	66	66	66
40	100	84	84	84
					50	100	92	92	92
CK	100	0	?	?

The test of giving birth to of four pairs of diamond-back moths of table 7 prescription is tested

Dosage (mg/l)	The examination borer population	Death toll	Lethality (%)	Corrected mortality (%)
					5	100	41	41	41
10	100	55	55	55
					20	100	78	78	78
40	100	95	95	95
					50	100	99	99	99
CK	100	0	?	?

The test of giving birth to of five pairs of diamond-back moths of table 8 prescription is tested

Dosage (mg/l)

The examination borer population

Death toll

Lethality (%)

Corrected mortality (%)

5	100	38	38	38
					10	100	53	53	53
20	100	66	66	66
					40	100	81	81	81
50	100	90	90	90
					CK	100	0	?	?

Analyze above-mentioned data, measurement result shows that when pleocidin and Bassa different proportion were composite, the co-toxicity coefficient of prescription all had obvious synergistic effect all greater than 120.Wherein fill a prescription four and the prescription five co-toxicity coefficient maximums, be 337.62 and 212.28.

By the indoor biometrics test, select the field trial that the bigger prescription of CTC four, prescription five and the less prescription one of CTC are prevented and treated diamond-back moth, determine the effect of prescription control diamond-back moth; Simultaneously by with the contrast of the effect of pleocidin, the agent of Bassa list, verify the synergistic effect after composite.Concrete test method, data are as follows:

Test one:10% Bassa, 7.5% pleocidin WDG, 15% Bassa WP, 2.5% pleocidin WDG do the test of pesticide effectiveness of field control diamond-back moth.Test site is Chen He town, Yingcheng City county, Xiaogan Prefecture, Hubei Province, and the time is on July 29th, 2010, test totally four processing, and each handles three repetitions, sub-district randomized arrangement, sub-district area 67m ², the planting habit of all sub-districts all planting habit with local is identical, and the record dispenser same day and the synoptic data in a week thereafter are specific as follows:

Table 9 duration of test meteorological data

The investigation standard is: concrete data (data are the mean value of three repetitions) are as follows:

The effect table of table 10 10% Bassa, 7.5% pleocidin WDG and other chemical control diamond-back moths

From above data as can be seen, under the situation of identical using dosage, 10% Bassa, 7.5% pleocidin 7d control rate behind medicine can reach 90.0%, the effect when preventive effect is higher than 10% Bassa and 7.5% pleocidin list agent use far away, and difference is extremely remarkable.

Test two:Test method, investigation method and meteorological data are the same, and concrete test data is as follows:

The effect table of table 11 15% Bassa, 5% pleocidin WDG and other chemical control diamond-back moths

From above-mentioned data as can be seen, under the situation of identical using dosage, 15% Bassa, 5% pleocidin 7d control rate behind medicine can reach 89.4%, the effect when preventive effect is higher than 15% Bassa and 5% pleocidin list agent use far away, and difference is extremely remarkable.

Test three:Test method, investigation method and meteorological data are the same, and concrete test data is as follows:

The effect table of table 12 25% Bassa, 1% pleocidin and other chemical control diamond-back moths

From above data as can be seen, under the situation of identical using dosage, 25% Bassa, 1% pleocidin 7d control rate behind medicine can reach 83.3%, the effect when preventive effect is higher than 25% Bassa and 1% pleocidin list agent use far away, and difference is extremely remarkable.

By above-mentioned three field control effectiveness tests, fill a prescription four as can be seen, the effect of prescription five control diamond-back moths is best, this also matches with the result of indoor biometrics, and (CTC is big more, synergistic effect is big more), but the two difference is not remarkable, as considering the factor of pesticide cost again, prescription four is best, the most economical proportionings of effect; Also verified simultaneously Bassa and pleocidin by different proportion when composite, the control efficiency of diamond-back moth all has been better than the effect of Bassa and pleocidin list agent use, certain synergistic effect has been arranged.

By above-mentioned three field control effectiveness tests, can determine to fill a prescription four for the more suitable prescription of control diamond-back moth, 10% Bassa, 7.5% pleocidin WDG(is filled a prescription four) and carried out further test, filter out the optium concentration of control diamond-back moth.Experimental scheme 10g/ mu, 12.5g/ mu, 15g/ mu and 4 concentration tonsures of 17.5g/ mu are also done contrast with the effect of 15% Bassa (20g/ mu), 2.5% pleocidin (60g/ mu), test method and investigation method are the same, and concrete data (mean values of three repetitions) are as follows:

Table 13 prescription four different amounts control diamond-back moth effect table

From the result of the above-mentioned test of pesticide effectiveness as can be seen, along with the increase of prescription dosage, drug effect also increases to some extent, 10% Bassa, 7.5% pleocidin WDG consumption is a 15g/ mu, the preventive effect of 7d is 89.9% behind the medicine, and apparently higher than 10% Bassa 20g/ mu and 7.5% pleocidin 15g/ mu, difference is extremely remarkable; When the mu consumption was 17.5g, 7 days preventive effect and 15g/ mu was roughly the same after medication, and difference is not remarkable.Therefore, under the prerequisite of considering the mu cost, should advocate mu usage amount is 15g/ mu.

Claims (3)

1. the Pesticidal combination of pleocidin and Bassa is characterized in that, described Pesticidal combination active component is made up of pleocidin and Bassa.

2. Pesticidal combination according to claim 1 is characterized in that, contains pleocidin 0.5%～10%, Bassa 1%～35%.

3. according to claim 1 or the described Pesticidal combination of claim 2, it is characterized in that described Pesticidal combination can be made into formulations such as suspending agent, wetting powder, water dispersible granules.