CN101897337A - Microbial pesticide synergist and microbial pesticide containing same - Google Patents
Microbial pesticide synergist and microbial pesticide containing same Download PDFInfo
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- CN101897337A CN101897337A CN2010102369366A CN201010236936A CN101897337A CN 101897337 A CN101897337 A CN 101897337A CN 2010102369366 A CN2010102369366 A CN 2010102369366A CN 201010236936 A CN201010236936 A CN 201010236936A CN 101897337 A CN101897337 A CN 101897337A
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- synergist
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- camptothecine
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Abstract
The invention belongs to the field of biotic insecticides and relates to a microbial pesticide synergist. The main ingredient of the synergist is camptothecin or a derivative thereof. The invention relates to two microbial pesticides containing the synergist at the same time, namely a microbial pesticide containing bacillus thuringiensis and the camptothecin/camptothecin derivative, and a microbial pesticide containing baculovirus and the camptothecin/camptothecin derivative. The synergist can effectively improve the pest killing effect of the bacillus thuringiensis or baculovirus pesticide, enhance the pesticide effect and reduce the dosage of the microbial pesticide while ensuring the same pest preventing and controlling effect; moreover, the active ingredient camptothecin of the synergist is a plant-originated substance which is safe for people and livestock and natural enemies of the pests, can slow down the pesticide resistance of the pests to the microbial pesticide and is easy to degrade without residue; and the synergist is suitable to be used as an environmental-friendly microbial pesticide synergist and has great significance for popularization and application of the microbial pesticide.
Description
Technical field
The invention belongs to biological insecticides and application thereof, relate to a kind of microbial pesticide synergist and contain the biopesticide of this synergist.
Background technology
Biopesticide is meant a class pesticidal preparations that utilizes biological living or its metabolite that pests such as insect, germ, weeds, nematode, muroid are prevented and treated; biopesticide comprises four classes such as microbial pesticide, botanical pesticide, animal sources agricultural chemicals and genetically modified organism agricultural chemicals; biopesticide has been compared following advantage with chemical pesticide: 1) selectivity is strong; safer to people and animals and various beneficial organism, protected natural enemy.2) little to eco-environmental impact, 3) can bring out insect epidemic disease etc.
Microorganism insecticide is the important part in the biopesticide, being to have lethal effect or pathogenic microbe groups to make an insecticides with its metabolite or microorganism itself to host insect, is bacterium class (Bt), virus type, Mycophyta (white muscardine fungi, green muscardine fungus), entomopathogenic nematode class for what people were familiar with at present.And bacterium class and virus type are to use two kinds of wider microorganism insecticides in producing.In the bacterium class, with bacillus thuringiensis,Bt (Bacillus thuringiensis, be called for short Bt) be representative, be present output maximum, a kind of microorganism insecticide that range of application is the widest, but the preparation that uses Bt usually exists shortcomings such as preventive effect instability, desinsection speed are slow, the longevity of residure is short, simultaneously also have the part insect that Bt has been produced resistance, this also is the problem that the Bt insecticide is faced; The baculovirus insecticides major defect is that desinsection speed is slow, needs just can make in 4～7 days the target pest death of infection as baculoviral.Because the defective of these aspects makes microorganism insecticide be very limited in applying.
Under this background, seek the synergistic component of synergistic matter as microbial pesticide, improve the insecticidal toxicity of microbial pesticide, reduce insect density, the generation that reduces the pest damage degree and delay pest resistance to insecticide, and synergistic component is easily degraded at natural world, environmentally safe, be the principle of selective advance composition, synergistic matter uses no insecticidal activity separately or has only extremely low insecticidal activity, can significantly improve the insecticidal activity of preparation in the time of in adding microorganism insecticide to.Yet the research and development of microbial pesticide synergist at present is still the link of a weakness in the microorganism insecticide application.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of synergist that can effectively promote microbial pesticide insecticidal activity and safety and environmental protection is provided.
The present invention is achieved through the following technical solutions above-mentioned purpose:
Invention provides a kind of microbial pesticide synergist, is main active ingredient with the camptothecine or derivatives thereof.
Camptothecine (Camptothecin is called for short CPT) is a kind of pyrroles's quinoline alkaloid, and the chemical structural formula of camptothecine is:
Camptothecine derives from a kind of alkaloid that extracts in the fruit, leaf of Nyssaceae plant camptotheca acuminata (Camptotheca acuminata Decne).Camplotheca acuminata is the Nyssaceae plant, has another name called Camptotheca acuminata, thousand sheets tree, Nanjing Chinese parasol tree etc., and it is the distinctive plant of China, is distributed widely in areas such as the Changjiang river, Chuan Nan, medically is being used for antineoplastic moral material.When using this camptothecine to be used to prevent and treat lepidopterous larvae separately, have only faint insecticidal action, but the present invention finds that camptothecine mixes use with microbial pesticide, then the latter is had remarkable synergistic effect.In to a collection of alkaloidal screening test, find, when adding in the microbial pesticide camptothecine or camptothecin derivative with the microbial pesticide coupling, its insecticidal effect is played obvious synergistic effect, can increase lethality and shorten the death time, can prevent that insect from continuing damage to crops, also can reduce the usage amount of microorganism insecticide simultaneously.
When using this microbial pesticide synergist, only need in microbial pesticide, add synergist, can be derivative with camptothecine or camptothecine, with dry powder or water-soluble after add in the microorganism insecticide, also can be before dispenser directly add in the microorganism insecticide.The weight ratio of microbial pesticide and camptothecine/camptothecin derivative is 0.5～5: 10～40 during application.
Except containing camptothecine, also contain wetting agent and filler in the synergist; Wetting agent can be selected from one or more in fatty alcohol-polyoxyethylene ether, lauryl sodium sulfate, neopelex, the Tea Saponin; Filler can be selected from one or several in diatomite, kaolin, wheat bran, potter's clay, calcium carbonate, the attapulgite.And the mass fraction of camptothecine in synergist is 0.5～8.5%.
The present invention discloses two kinds the microbial pesticide that contains this synergist.
First thuringiensis cladosporioides bacillus insecticide contains the camptothecine or derivatives thereof simultaneously in this agricultural chemicals.Wherein the weight ratio of camptothecine/camptothecin derivative and bacillus thuringiensis is 0.5～5: 10～40.Remove this, can also contain above-mentioned wetting agent and filler in this agricultural chemicals.The mass fraction of each material is: bacillus thuringiensis dry powder 10～40%; Camptothecine/camptothecin derivative 0.5～5%; Wetting agent 3～10%; Filler 45～67.5%.
It two is baculovirus insecticides, contains the camptothecine or derivatives thereof in this agricultural chemicals simultaneously.Wherein the weight ratio of camptothecine/camptothecin derivative and baculoviral is 0.2～3: 5～30.Remove this, can also contain above-mentioned wetting agent and filler in this agricultural chemicals.The mass fraction of each material is: baculovirus polyhedrin body 5～30%; Camptothecine/camptothecin derivative 0.1～3%; Wetting agent 3～10%; Filler 57～91.8%.
Invention shows through a large amount of tests, after the insecticidal crystal protein of camptothecine and Bt or baculovirus polyhedrin body are mixed, synergistic effect to two kinds of microorganism insecticides is remarkable, and can prolong the longevity of residure of Bt, with the lethal time of shortening baculoviral to insect, prevent that insect from continuing damage to crops, under equal preventive effect, reduce the usage amount of microorganism insecticide.
Compared with prior art, the present invention has following characteristics:
1. synergist provided by the present invention can promote the insecticidal effect of microorganism insecticide effectively, improves drug effect, can reduce the consumption of microorganism insecticide under equal preventive effect.
2. the present invention is that the synergist camptothecine that is provided also is a kind of plant resource material, during as the synergistic component of biopesticide, and easily degraded, noresidue, the biopesticide synergist that is suitable as a kind of environmental protection is promoted the use of.
3. synergist used in the present invention can delay insect to the drug-fast generation of microorganism insecticides such as Bt, and is significant for the use of applying microorganism insecticide, the medication of minimizing high residue chemistry.
4. Bt or the baculovirus insecticides insecticidal toxicity that contains camptothecine provided by the present invention is higher 2～3 times than its independent use.
5. this invention preparation method is simple, raw material sources are extensive, with low cost.
Embodiment
In order to understand essence of the present invention better, below with the technology contents of example in detail invention, but the content of invention is not limited thereto.
Embodiment 1: camptothecine is mass ratio in synergist:
Camptothecine: 3%
Wetting agent: neopelex 10%
Filler: diatomite 87%
Embodiment 2: the mass ratio that contains camptothecine and be each component in the Bt wetting powder of synergistic component:
Bacillus thuringiensis,Bt dry powder: 20%
Camptothecine: 0.3%
Neopelex: 5%
Kaolin is mended to 100%
Embodiment 3: the mass ratio of each component that contains camptothecine and be the bacillus thuringiensis suspending agent of synergistic component:
Bacillus thuringiensis,Bt dry powder: 20%
Camptothecine: 0.3%
Tea Saponin 3%
Calcium carbonate 76.7%
Embodiment 4: containing camptothecine is the preparation weight ratio of the baculovirus insecticides wetting powder of synergistic component:
Baculovirus polyhedrin body 10%,
Camptothecine 0.2%,
Wetting agent neopelex 5%,
Kaolin is mended to 100%
Embodiment 5
Take by weighing Bt standard items (15000IU/mg) sample 5g, camptothecine 0.6g mixes, use this prescription with 2000 times of liquid of its dilution, 72 hours lethality to the cabbage loopers of 3 age ends reach 100%, and use Bt to have only 30% separately, use camptothecine lethality＜5% separately.
Embodiment 6:
Take by weighing Bt standard items (15000IU/mg) sample 5g, camptothecine 0.6g mixes, 2000 times of liquid of dilute with water are used to prevent and treat the beet armyworm in 1～2 age, and control efficiency reached more than 85% in 72 hours, and be 40% for independent use Bt, using camptothecine separately is 20%.
Embodiment 7:
Take by weighing Bt standard items (15000IU/mg) sample 5g, camptothecine 0.2g mixes, 4000 times of liquid of dilute with water are used to prevent and treat the diamond-back moth in 3 ages, and control efficiency reached more than 80% in 24 hours, and be 40% for independent use Bt, using camptothecine separately is 20%.
Embodiment 8:
Get that to contain the baculovirus polyhedrin bulk concentration be 10
4ACMNPV, to wherein adding the camptothecine that final concentration is 0.06g/L, be used to prevent and treat the cabbage looper in 4 ages, control efficiency reached 95.8% in 144 hours, and used 10 separately
4The ACMNPV control efficiency be 37.5%, use camptothecine lethality 21% separately.
Claims (10)
1. a microbial pesticide synergist is characterized in that synergist is main active ingredient with the camptothecine or derivatives thereof.
2. microbial pesticide synergist as claimed in claim 1 is characterized in that the mass fraction of described camptothecine or derivatives thereof in synergist is 0.5～8.5%.
3. microbial pesticide synergist as claimed in claim 1 is characterized in that described synergist also contains wetting agent and filler; Described wetting agent is one or more in fatty alcohol-polyoxyethylene ether, lauryl sodium sulfate, neopelex, the Tea Saponin; Described filler is one or several in diatomite, kaolin, wheat bran, potter's clay, calcium carbonate, the attapulgite.
4. the application of a microbial pesticide synergist as claimed in claim 1 is characterized in that adding the camptothecine or derivatives thereof in microbial pesticide, and wherein the weight ratio of microbial pesticide and camptothecine/camptothecin derivative is 5～40: 0.1～5.
5. a microbial pesticide that contains synergist is characterized in that containing bacillus thuringiensis, contains the camptothecine or derivatives thereof simultaneously.
6. microbial pesticide as claimed in claim 5, the weight ratio that it is characterized in that camptothecine/camptothecin derivative and bacillus thuringiensis is 0.5～5: 10～40.
7. microbial pesticide as claimed in claim 6 is characterized in that also containing wetting agent and filler, and the mass fraction of each material is: bacillus thuringiensis 10～40%; Camptothecine/camptothecin derivative 0.5～5%; Wetting agent 3～10%; Filler 45～86.5%.
8. a microbial pesticide that contains synergist is characterized in that containing wild-type baculovirus or recombinant type baculovirus polyhedrin body or their combination, contains the camptothecine or derivatives thereof simultaneously.
9. microbial pesticide as claimed in claim 8, the weight ratio that it is characterized in that camptothecine/camptothecin derivative and baculovirus polyhedrin body is 0.2～3: 5～30.
10. microbial pesticide as claimed in claim 9 is characterized in that also containing wetting agent and filler, and the mass fraction of each material is: baculovirus polyhedrin body 5～30%; Camptothecine/camptothecin derivative 0.2～3%; Wetting agent 3～10%; Other filler 57～91.8%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010236936 CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010236936 CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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| CN101897337A true CN101897337A (en) | 2010-12-01 |
| CN101897337B CN101897337B (en) | 2013-05-22 |
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| CN 201010236936 Expired - Fee Related CN101897337B (en) | 2010-07-23 | 2010-07-23 | Microbial pesticide synergist and microbial pesticide containing same |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275095A (en) * | 2013-05-08 | 2013-09-04 | 中国农业科学院植物保护研究所 | 10-hydroxycamptothecine derivatives and applications thereof |
| CN109511680A (en) * | 2018-11-27 | 2019-03-26 | 何美英 | A kind of biological pesticide and preparation method thereof of the fatty glyceride of Residual oil containing rice residue |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883267A (en) * | 2006-05-31 | 2006-12-27 | 浙江林学院 | Agricultural bactericide containing camptothecin or camptothecin derivative and application thereof |
| CN101299919A (en) * | 2005-11-01 | 2008-11-05 | 美国陶氏益农公司 | Pesticidally active compositions having enhanced activity |
-
2010
- 2010-07-23 CN CN 201010236936 patent/CN101897337B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101299919A (en) * | 2005-11-01 | 2008-11-05 | 美国陶氏益农公司 | Pesticidally active compositions having enhanced activity |
| CN1883267A (en) * | 2006-05-31 | 2006-12-27 | 浙江林学院 | Agricultural bactericide containing camptothecin or camptothecin derivative and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103275095A (en) * | 2013-05-08 | 2013-09-04 | 中国农业科学院植物保护研究所 | 10-hydroxycamptothecine derivatives and applications thereof |
| CN103275095B (en) * | 2013-05-08 | 2015-07-22 | 中国农业科学院植物保护研究所 | 10-hydroxycamptothecine derivatives and applications thereof |
| CN109511680A (en) * | 2018-11-27 | 2019-03-26 | 何美英 | A kind of biological pesticide and preparation method thereof of the fatty glyceride of Residual oil containing rice residue |
| CN109511680B (en) * | 2018-11-27 | 2021-12-07 | 上海沪联生物药业（夏邑）股份有限公司 | Biopesticide containing rice residue oil and fatty acid glyceride and preparation method thereof |
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| CN101897337B (en) | 2013-05-22 |
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