CN106508997A - Microorganism wettable powder for preventing pine caterpillars and preparation method thereof - Google Patents
Microorganism wettable powder for preventing pine caterpillars and preparation method thereof Download PDFInfo
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- CN106508997A CN106508997A CN201610927014.7A CN201610927014A CN106508997A CN 106508997 A CN106508997 A CN 106508997A CN 201610927014 A CN201610927014 A CN 201610927014A CN 106508997 A CN106508997 A CN 106508997A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses microorganism wettable powder for preventing pine caterpillars. The microorganism wettable powder comprises the following raw materials including, by weight, 30-40 parts of catharanthus roseus, 22-36 parts of veratrum nigrum, 15-27 parts of aconite, 14-20 parts of pomelo peels, 8-12 parts of beauveria bassiana powder, 4-10 parts of bacillus popillae powder, 4-8 parts of wetting agents, 6-14 parts of dispersing agents, 5-10 parts of disintegrating agents and 50-60 parts of fillers. The microorganism wettable powder is prepared through the steps that, beauveria bassiana and bacillus papillae are used as microbial insecticide, meanwhile, the four kindes of processed botanical insecticide of the catharanthus roseus, the veratrum nigrum, the aconite and the pomelo peels are added, and moderate auxiliary materials are added; the microbial insecticide and the botanical insecticide act synergistically and supplement each other; and microorganisms, metabolites of the microorganisms and insecticidal active components of botanical pesticide are reasonably utilized and used specially for the pine caterpillars, the utilization rate is high, and the control effect is good.
Description
Technical field
The present invention relates to a kind of agricultural chemicals and preparation method thereof, more particularly, to a kind of microorganism wettable of preventing and treating pine moth
Pulvis and preparation method thereof.
Background technology
Wettable powder is, with pesticide original medicine, inert filler and a certain amount of auxiliary agent, to be adequately mixed after crushing in proportion,
Reach the formulation of certain particle size.From in shape, and pulvis indistinction, but due to adding wetting agent, dispersant etc.
Auxiliary agent, can be readily wetted by water after being added in water, disperse, form suspension, sprayable administration.Pine moth is category Lepidoptera Lasiocampidae
The general designation of Dendrolimus insect, also known as caterpillar, fiery caterpillar, food evil Pinaceae, Cupressaceae are that generating capacity is big in China injurious forest-insect, danger
The wide Main Forest Insect Pests of evil.
The Chinese patent of Application No. 200810197522X, disclosing a kind of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus can bacillus
Wettable powder for disinsection and preparation method, in the formulation, Main Ingredients and Appearance is Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus and Dipel,
Wherein Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus inclusion body content 5.0 × 106-1.0×109PIB/g, Dipel content 15-89%.
And add appropriate white carbon, mica powder, sodium metnylene bis-naphthalene sulfonate, lauryl sodium sulfate and sodium lignin sulfonate etc. to help
Agent.The formula is screened through science, and insecticidal spectrum is wide, and easy to operate, prevention effect has reached more than 75%.The invention is given birth to environment
Thing, person poultry harmless, no environmental pollution, non agricultural chemical residuum, the generation and harm of energy effective control pine moth are suitable on pine tree big
Amount is applied.Accelerate insect virus insecticide speed again simultaneously, ask during the desinsection for making viral insecticide and shorten to 2- 3 days.Application number
For 201210170558.5 Chinese patent, the insecticides and purposes of a kind of preventing and treating agriculture and forestry injurious insect are disclosed, belongs to day
Right technical field of insecticide, it is characterised in that containing tabasheer extract 15-37%, fungi 3-15%, stabilizer 3-11%, bleeding agent
1-9%, solvent 39-65%.The insecticides of above-mentioned a kind of preventing and treating agriculture and forestry injurious insect, with pure natural tabasheer extract as activity
Composition, addition can directly infect various fungies that insect body wall kills off the insect pests and are prepared from very strong, to pine moth, snout moth's larva
The agriculture and forestry injurious insects such as worm, diamondback moth, wooden poison moth, aphid, smaller green leaf hopper, crab toad, Monochamus alternatus have it is very strong kill effect, can have
Effect reduces the generation of agricultural pest and disease damage.
The problem that above-mentioned patent is present is：Pine moth is prevented and treated using microbial insecticide merely, insecticidal mechanism list
First, insecticidal effect is not good.
The content of the invention
In view of this, the purpose of the present invention is for the deficiencies in the prior art, there is provided a kind of microorganism of preventing and treating pine moth
Wettable powder and preparation method thereof, solves the problems, such as that current microbial insecticide mechanism is single, insecticidal effect is not good.
To reach above-mentioned purpose, the present invention is employed the following technical solutions：
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：Catharanthus roseus 30-40 parts, black false hellebore
22-36 parts, rhizome of Chinese monkshood 15-27 parts, pomelo peel 14-20 parts, muscardine powder 8-12 parts, chafer bacillus powder 4-10 parts,
Wetting agent 4-8 parts, dispersant 6-14 parts, disintegrant 5-10 parts and filler 50-60 parts.
Preferably, the Microbial wettable powder of described preventing and treating pine moth, including the raw material of following weight portion：Catharanthus roseus
35 parts, 29 parts of black false hellebore, 21 parts of the rhizome of Chinese monkshood, 17 parts of pomelo peel, 10 parts of muscardine powder, 7 parts of chafer bacillus powder, wetting agent
55 parts of 6 parts, 10 parts of dispersant, 7 parts of disintegrant and filler.
Preferably, effective content >=2 × 10 of the muscardine powder8cfu/g；The chafer bacillus powder
Effective content >=2 × 108cfu/g。
Preferably, the wetting agent is in sodium lignin sulfonate, cetyl benzenesulfonic acid sodium and sulfo fatty acid ester
It is a kind of；
The one kind of the disintegrant in ammonium sulfate, sodium sulphate and urea；
The one kind of the dispersant in maleic anhydride oligomers and fatty alcohol sulfate salt.
Preferably, the one kind of described filler in sepiolite, bentonite and diatomite.
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 40-80 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 12-18 times is 60-80% second
Alcoholic solution, ultrasonic extraction 4-8h, supersonic frequency 20-30KHz are collected extract, are concentrated into without alcohol taste, medicine is obtained final product after vacuum drying
Thing extract；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 300-500 mesh, obtain final product product.
Preferably, the step（1）In, the temperature of ultrasonic extraction is 20-40 DEG C.
The invention has the beneficial effects as follows：
The wettable powder of the present invention is with muscardine and chafer bacillus as microbial insecticide, while adding through processing
Catharanthus roseus, black false hellebore, the rhizome of Chinese monkshood and pomelo peel Four Plants insecticide add appropriate auxiliary material and be prepared from, microbial insecticide
Agent and plant insecticide synergy, both complement each other, and rationally utilize microorganism and its metabolite and plant source agriculture
Active insecticidal components in medicine, are specifically designed for pine moth, and utilization rate is high, prevention effect is good, specifically have it is following some：
（1）The wettable powder that the present invention is prepared, the microorganism that effective insecticidal constituent therein comes from nature are killed
Worm agent and plant insecticide, it is to avoid in currently available technology insecticide use substantial amounts of organic agricultural chemicals and to natural environment and
The ecological balance works the mischief, and one side microbial insecticide is strong to insect action specificity, few pest natural enemy and beneficial of killing
Biology, does not destroy the ecological balance, and plant insecticide is easy to gather materials on the spot production and application from agricultural byproducts, is easy to rural area to push away
Extensively, low toxicity is efficient.
（2）Wettable powder low production cost prepared by the present invention, accumulating is convenient, safety, and packaging material are easier place
Reason, and solvent and emulsifying agent are not used, it is safer to plant, poisoning is not likely to produce, it is environmentally safe.
（3）In wettable powder prepared by the present invention, on the one hand add muscardine powder and chafer bacillus powder
Used as microbial insecticide, used as a kind of fungus insecticide, its insecticidal mechanism is wherein muscardine：After spore touches polypide,
Infected by the alimentary canal and body wall of insect, run into suitable temperature and humidity and sprout, form mycelia, mycelia can be penetrated worm
Body wall is stretched in vivo, is bred, and secretes chitinase and archon, and dissolving insect body wall invades polypide, metabolite
Accumulated in insect body in a large number, cause the change of insect physicochemical character, ultimately result in insect metabolic disturbance and dead；Cockchafer
The insecticidal mechanism of sub- bacillus is：Chafer bacillus is sprouted in middle enteral after being eaten by larva, intestines in then invading
Body cavity is entered after in cylindricality cell, is caused the tissue change in insect body and physiological variation, is ultimately resulted in death.
On the other hand, addition can be used as the catharanthus roseus of plant insecticide, black false hellebore, the rhizome of Chinese monkshood and pomelo peel, wherein catharanthus roseus
Containing more than 70 kinds alkaloids, mainly there are vincaleukoblastinum, vincristine, vinblastine, the chemical substance such as peaceful in Changchun, which is cool in nature, taste
Slight bitter, can cool blood step-down, tranquilizing and allaying excitement has various toxic reactions for hypertension, burning hot wound, malignant lymphoma, and right
Insect has action of contace poison；
Black false hellebore, Liliaceae Veratrum perennial herb, with medical function, taste is pungent, hardship are cold in nature, poisonous, with anemophlegmatic vomiting,
Desinsection treat sore, eliminating the phlegm, emetic, effect of desinsection, can be used for treat apoplexy, angioleucitis, mastitis, fracture, favus of the scalp, scabies with
And maggot of going out, fly etc.；
Double lipo-alkaloids of the severe toxicity such as rhizome of Chinese monkshood mesaconitine, Hypaconitine, isoaconitine, except having to the various cancer cells of human body
Outside direct killing and inhibitory action, nervous system is had an effect on so that restlessness, limbs are hardened, muscle rigidity, twitch until dead
Die；
Swab skin is Rutaceae fruit shaddock crust, wherein containing multiple biological activities composition, such as taking out skin former times, essential oil, orange
Skin glycosides etc. have distinguishingly, overpowering odor, modern study shows that these bioactive ingredients in pomelo peel have to insect
The effect of keeping away and anti-oxidant and broad spectrum antibacterial performance, can serve as insecticide.
Therefore, the wettable powder that prepared by the present invention, first addition can suppress the tubulin of pine moth to be formed and damaged
Pine moth Nerve conduction, the catharanthus roseus of the movement function of muscle and the rhizome of Chinese monkshood, add and have action of contace poison to pine moth
Black false hellebore and the pomelo peel with avoidance effect, progressively cause irreversible infringement, cause between its neuro-muscular to pine moth
There is obstacle, muscle and acutely shrinks, loses the ability of looking for food in information transmission, and secondly addition can be colonized in pine moth body and to pine tag
Muscardine and chafer bacillus that worm damages, cause pine moth tissue and organ deficiency, two kinds of different disinfesting machines
System, six kinds of different material synergies, is caused damage to pine moth from different perspectives, ultimately results in pine moth death, desinsection effect
Rate is efficient.
（4）In wettable powder agent prescription prepared by the present invention, filler in sepiolite, bentonite and diatomite
Kind, three kinds of carriers are a kind of hydrosilicate clay minerals of pure natural, nontoxic, tasteless, "dead" element, nonmetallic
Possesses the content pore passage structure of relatively large specific surface area and uniqueness in mineral, on the one hand high adsorption capacity effectively can be inhaled
Effective insecticidal constituent in attached pulvis, on the other hand, after being administered to pine forest, also has preferable adhesive force to pine moth, therefore
The utilization rate of medicine is high, good disinsection effect.
（5）In daily life, pomelo peel Jing does not obtain reasonable profit frequently as garbage disposal, the value of its antibacterial, worm-expelling
With, causing greatly to waste, in wettable powder agent prescription prepared by the present invention, add appropriate pomelo peel, Jing process is effective by which
Composition realizes resource reasonable employment, turns waste into wealth, with the meaning promoted the use of as one of component for killing insect pine moth
Justice.
Specific embodiment
With reference to specific embodiment, the invention will be further described, wherein the bacterial classification of the present invention belongs to existing skill
Art, commercially can obtain.
Embodiment 1
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：30 parts of catharanthus roseus, black false hellebore 36
Part, 15 parts of the rhizome of Chinese monkshood, 20 parts of pomelo peel, 8 parts of muscardine powder, 4 parts of chafer bacillus powder, 8 parts of wetting agent, dispersant 6
50 parts of part, 10 parts of disintegrant and filler, wherein, wetting agent is sodium lignin sulfonate, and dispersant is maleic anhydride oligomers, disintegration
Agent is ammonium sulfate, and filler is sepiolite.
Preferably, the effective content of the muscardine powder is 2 × 108cfu/g；The chafer bacillus powder
Effective content is 2 × 108cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 80 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 18 times is 60% ethanol solution,
Ultrasonic extraction 8h at 20 DEG C, supersonic frequency 30KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 500 mesh, obtain final product product.
Embodiment 2
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：40 parts of catharanthus roseus, black false hellebore 22
Part, 27 parts of the rhizome of Chinese monkshood, 14 parts of pomelo peel, 12 parts of muscardine powder, 10 parts of chafer bacillus powder, 4 parts of wetting agent, dispersant
60 parts of 14 parts, 5 parts of disintegrant and filler, wherein, wetting agent is cetyl benzenesulfonic acid sodium, and dispersant is fatty alcohol sulfate
Salt, disintegrant are urea, and filler is bentonite.
Wherein, the effective content of the muscardine powder is 2 × 109cfu/g；The chafer bacillus powder has
Effect content is 2 × 1010cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 80 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 12 times is 80% ethanol solution,
Ultrasonic extraction 4h at 40 DEG C, supersonic frequency 20KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 300 mesh, obtain final product product.
Embodiment 3
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：35 parts of catharanthus roseus, black false hellebore 29
Part, 21 parts of the rhizome of Chinese monkshood, 17 parts of pomelo peel, 10 parts of muscardine powder, 7 parts of chafer bacillus powder, 6 parts of wetting agent, dispersant
55 parts of 10 parts, 7 parts of disintegrant and filler, wherein, wetting agent is sodium lignin sulfonate, and dispersant is maleic anhydride oligomers, is collapsed
Solution agent is urea, and filler is sepiolite.
Preferably, the effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder
Effective content is 2 × 1010cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 60 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 16 times is 70% ethanol solution,
Ultrasonic extraction 6h at 30 DEG C, supersonic frequency 30KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 400 mesh, obtain final product product.
Embodiment 4
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：35 parts of catharanthus roseus, black false hellebore 29
Part, 21 parts of the rhizome of Chinese monkshood, 17 parts of pomelo peel, 10 parts of muscardine powder, 7 parts of chafer bacillus powder, 6 parts of wetting agent, dispersant
55 parts of 10 parts, 7 parts of disintegrant and filler, wherein, wetting agent is sodium lignin sulfonate, and dispersant is maleic anhydride oligomers, is collapsed
Solution agent is urea, and filler is modified meerschaum.
The preparation method of wherein modified meerschaum is that sepiolite is put into the lignin sulfonic acid sodium solution that mass concentration is 6%
Middle heating stirring 12, suction filtration are collected filter cake and are washed with deionized 5 times, dry, naturally cool to room temperature, be crushed to 300
Mesh, obtains final product modified meerschaum.
Preferably, the effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder
Effective content is 2 × 1010cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 60 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 16 times is 70% ethanol solution,
Ultrasonic extraction 6h at 30 DEG C, supersonic frequency 30KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 400 mesh, obtain final product product.
Embodiment 5
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：38 parts of catharanthus roseus, black false hellebore 34
Part, 17 parts of the rhizome of Chinese monkshood, 19 parts of pomelo peel, 9 parts of muscardine powder, 8 parts of chafer bacillus powder, 7 parts of wetting agent, dispersant 12
53 parts of part, 5 parts of disintegrant and filler, wherein, wetting agent is cetyl benzenesulfonic acid sodium, and dispersant is fatty alcohol sulfate salt,
Disintegrant is sodium sulphate, and filler is diatomite.
Preferably, the effective content of the muscardine powder is 2 × 108cfu/g；The chafer bacillus powder
Effective content is 2 × 109cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 7 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 15 times is 80% ethanol solution,
Ultrasonic extraction 7h at 30 DEG C, supersonic frequency 20KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 300 mesh, obtain final product product.
Embodiment 6
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：34 parts of catharanthus roseus, black false hellebore 26
Part, 23 parts of the rhizome of Chinese monkshood, 18 parts of pomelo peel, 10 parts of muscardine powder, 5 parts of chafer bacillus powder, 5 parts of wetting agent, dispersant 9
58 parts of part, 9 parts of disintegrant and filler, wherein, wetting agent is sodium lignin sulfonate, and dispersant is fatty alcohol sulfate salt, is disintegrated
Agent is ammonium sulfate, and filler is bentonite.
Preferably, the effective content of the muscardine powder is 2 × 108cfu/g；The chafer bacillus powder
Effective content is 2 × 109cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 50 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 14 times is 75% ethanol solution,
Ultrasonic extraction 8h at 30 DEG C, supersonic frequency 20KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 500 mesh, obtain final product product.
Embodiment 7
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：36 parts of catharanthus roseus, black false hellebore 24
Part, 19 parts of the rhizome of Chinese monkshood, 16 parts of pomelo peel, 11 parts of muscardine powder, 6 parts of chafer bacillus powder, 8 parts of wetting agent, dispersant 8
56 parts of part, 8 parts of disintegrant and filler, wherein, wetting agent is cetyl benzenesulfonic acid sodium, and dispersant is fatty alcohol sulfate salt,
Disintegrant is sodium sulphate, and filler is bentonite.
Preferably, the effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder
Effective content is 2 × 109cfu/g。
The preparation method of the Microbial wettable powder of above-mentioned preventing and treating pine moth, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 40 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 17 times is 80% ethanol solution,
Ultrasonic extraction 8h at 20 DEG C, supersonic frequency 20KHz are collected extract, are concentrated into without alcohol taste, and medicine extraction is obtained final product after vacuum drying
Thing；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 500 mesh, obtain final product product.
Embodiment 8
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：32 parts of catharanthus roseus, black false hellebore 32
Part, 25 parts of the rhizome of Chinese monkshood, 20 parts of pomelo peel, 12 parts of muscardine powder, 9 parts of chafer bacillus powder, 4 parts of wetting agent, dispersant
59 parts of 10 parts, 6 parts of disintegrant and filler, wherein, wetting agent is sodium lignin sulfonate, and dispersant is maleic anhydride oligomers, is collapsed
Solution agent is ammonium sulfate, and filler is sepiolite.
Preferably, the effective content of the muscardine powder is 2 × 108cfu/g；The chafer bacillus powder
Effective content is 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 5 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 1
Comparative example 1 is substantially the same manner as Example 3, difference be 1 wettable powder of comparative example formula in without catharanthus roseus.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：39 parts of black false hellebore, the rhizome of Chinese monkshood 31
Part, 27 parts of pomelo peel, 13 parts of muscardine powder, 9 parts of chafer bacillus powder, 6 parts of wetting agent, 10 parts of dispersant, disintegration
55 parts of 7 parts of agent and filler, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urine
Element, filler are sepiolite.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 2
Comparative example 2 is substantially the same manner as Example 3, difference be 2 wettable powder of comparative example formula in without black false hellebore.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：45 parts of catharanthus roseus, the rhizome of Chinese monkshood
26 parts, 22 parts of pomelo peel, 15 parts of muscardine powder, 11 parts of chafer bacillus powder, 6 parts of wetting agent, 10 parts of dispersant, collapse
55 parts of 7 parts of solution agent and filler, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urine
Element, filler are sepiolite.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 3
Comparative example 3 is substantially the same manner as Example 3, difference be 3 wettable powder of comparative example formula in without the rhizome of Chinese monkshood.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：40 parts of catharanthus roseus, black false hellebore
34 parts, 22 parts of pomelo peel, 13 parts of muscardine powder, 10 parts of chafer bacillus powder, 6 parts of wetting agent, 10 parts of dispersant, collapse
55 parts of 7 parts of solution agent and filler, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urine
Element, filler are sepiolite.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 4
Comparative example 4 is substantially the same manner as Example 3, difference be 4 wettable powder of comparative example formula in without pomelo peel.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：40 parts of catharanthus roseus, black false hellebore
34 parts, 24 parts of the rhizome of Chinese monkshood, 12 parts of muscardine powder, 9 parts of chafer bacillus powder, 6 parts of wetting agent, 10 parts of dispersant, disintegration
55 parts of 7 parts of agent and filler, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urine
Element, filler are sepiolite.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 5
Comparative example 5 is substantially the same manner as Example 3, difference be 5 wettable powder of comparative example formula in without Beauveria Bassiana Powder
End.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：37 parts of catharanthus roseus, black false hellebore
31 parts, 23 parts of the rhizome of Chinese monkshood, 19 parts of pomelo peel, 9 parts of chafer bacillus powder, 6 parts of wetting agent, 10 parts of dispersant, 7 parts of disintegrant
With 55 parts of filler, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urea, filler
For sepiolite.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 6
Comparative example 6 is substantially the same manner as Example 3, difference be 6 wettable powder of comparative example formula in without chafer bud
Spore bacillus powder.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：37 parts of catharanthus roseus, black false hellebore
31 parts, 22 parts of the rhizome of Chinese monkshood, 18 parts of pomelo peel, 11 parts of muscardine powder, 6 parts of wetting agent, 10 parts of dispersant, 7 parts of disintegrant and filler
55 parts, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urea, and filler is Hai Pao
Stone.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Comparative example 7
Comparative example 7 is substantially the same manner as Example 3, difference be 7 wettable powder of comparative example formula in no-arbitrary pricing.
A kind of Microbial wettable powder of preventing and treating pine moth, including the raw material of following weight portion：35 parts of catharanthus roseus, black false hellebore
29 parts, 21 parts of the rhizome of Chinese monkshood, 17 parts of pomelo peel, 10 parts of muscardine powder, 7 parts of chafer bacillus powder, 6 parts of wetting agent, dispersion
7 parts of 10 parts of agent and disintegrant, wherein, wetting agent is sulfo fatty acid ester, and dispersant is maleic anhydride oligomers, and disintegrant is urine
Element.
The effective content of the muscardine powder is 2 × 1010cfu/g；The chafer bacillus powder effectively contains
Measure as 2 × 1010cfu/g。
The preparation of wettable powder in the preparation method and embodiment 3 of the Microbial wettable powder of above-mentioned preventing and treating pine moth
Method is identical.
Physicochemical property is tested
The Microbial wettable powder that preventing and treating pine moth is prepared to embodiment 1-8 and comparative example 1-7 carries out physicochemical property inspection
Survey, including appearance character, disintegration time, suspensibility and heat storage stability, wherein disintegration time assay method will be prepared
In the Microbial wettable powder input tank of preventing and treating pine moth, water layer thickness is 7cm, and wettable powder enters water and starts timing,
Terminate until being completely dispersed timing；Suspensibility determines the method using the regulation in GB/T 14825-2006, and heat storage stability is adopted
With method specified in 19136 standards of GB/T, represented using the height of resolution ratio, resolution ratio is lower, shows heat storage stability
Better.
Insect killing effect test
The Microbial wettable powder of preventing and treating pine moth prepared by embodiment 1-8 and comparative example 1-7 is respectively with water according to 1:
1000 quality proportioning mixing, obtains liquid, and insect killing effect test the results are shown in Table 2.
Test method is according to People's Republic of China's agricultural industry criteria《Farm-chemical indoor determination test rule》Desinsection
In agent NYT 1154.6-2006, the dip method of the 6th part is carried out.From the larva of pine moth, each embodiment or comparative example are matched somebody with somebody
The liquid of system processes each kind 80, and processing method is target pest to be soaked after into the liquid 10s, draws unnecessary medicine with filter paper
Liquid, test worm is transferred under normal condition and is raised, and arrange the aqueous solution without wettable powder liquid as a control group, respectively
Test worm The dead quantity the death rate and corrected mortality are calculated during statistic record 3 days and 5 days.
The death rate（%）=dead test worm number/total test worm number × 100%
Corrected mortality（%）=（The death rate-control the death rate）/（1- compares the death rate）×100%
From the physicochemical property test result table 1 and insect killing effect test result table 2 of above-described embodiment 1-8：The system of the present invention
It is 1.5-4min that the Microbial wettable powder of the standby preventing and treating pine moth for obtaining is single-size, disintegration time, and disintegration is rapid, can
Quickly to form stable solution, suspensibility is 90.2%-96.4%, and resolution ratio is 1.5%-3.6%, and its physics and chemistry is not only conformed with
Relevant national standard, and wettable powder excellent in stability itself, the liquid medicine stability formed after molten water are excellent, active ingredient
Suspensibility it is high；Pine moth larva is directed to simultaneously, is tested using dip method, death rate when as a result showing pine moth larva 3d
75.00%-81.25%, 74.68%-81.01% are respectively with corrected mortality, the death rate and correction are dead during pine moth larva 5d
Rate is respectively 88.75%-96.25%, 88.46%-96.15%, illustrates that the wettable powder of the present invention is directed to pine moth prevention effect
It is excellent, to pine moth produce orientation killing action, while effective insecticidal constituent of wettable powder come from it is micro- in nature
Biological insecticides and plant insecticide, it is to avoid in currently available technology insecticide use substantial amounts of organic agricultural chemicals and to nature
Environment and the ecological balance work the mischief, and microbial insecticide is strong to insect action specificity, will not kill pest natural enemy and
Beneficial organism, does not destroy the ecological balance, and plant insecticide is easy to gather materials on the spot production and application from agricultural byproducts, is easy to agriculture
Village promotes, and low toxicity is efficient.
With reference to Tables 1 and 2, from embodiment 4 and embodiment 3：With the addition of in 4 wettable powder agent prescription of embodiment modified
Sepiolite is used as filler, as shown in Table 1, after sepiolite adopts wooden sodium sulfonate's modification, the adsorption capacity on sepiolite surface
Significantly increase, the suspensibility rising of wettable powder active ingredient, resolution ratio are reduced, and illustrate wettable powder prepared by embodiment 4
Agent performance is more stable, and the death rate and corrected mortality of 4 pine moth of embodiment is improved as shown in Table 2, illustrates sepiolite
The modified suction-operated for active ingredient strengthens, therefore insecticidal effect is more excellent.
With reference to Tables 1 and 2, from embodiment 3 and comparative example 1-6, Changchun in the formula of comparative example 1-6, is eliminated respectively
Flower, black false hellebore, the rhizome of Chinese monkshood, pomelo peel, muscardine powder or chafer bacillus powder, the wettable powder for preparing is for pine
The insecticidal effect of caterpillar is substantially reduced, the Microbial wettable powder of the preventing and treating pine moth of this explanation present invention, with muscardine and
Chafer bacillus is microbial insecticide, while the treated catharanthus roseus of addition, black false hellebore, the four kinds of plants of the rhizome of Chinese monkshood and pomelo peel
Thing insecticide simultaneously adds appropriate auxiliary material and is prepared from, and catharanthus roseus and the rhizome of Chinese monkshood can suppress the tubulin of pine moth to be formed and damaged
Evil pine moth Nerve conduction, the movement function of muscle, black false hellebore have action of contace poison to pine moth, and pomelo peel has to pine moth
There are avoidance effect, muscardine and chafer bacillus colonize in pine moth body and damage to pine moth, cause pine
Caterpillar is organized and organ deficiency, two kinds of different insecticidal mechanisms, six kinds of material synergies, and from different perspectives pine moth is caused
Infringement, ultimately results in pine moth death, and insecticide efficiency is efficient.
With reference to Tables 1 and 2, from embodiment 3 and comparative example 7, in the formula of comparative example 7, filler is eliminated, can by table 1
Know, comparative example 7 prepare for irregular particle, while the suspensibility that disintegration time significantly extends with effect composition is reduced,
As shown in Table 2, in comparative example 7 during pine moth 3d, during 5d, corrected mortality is respectively 60.76%, 67.95%, pine tag in embodiment 3
During worm 3d, 5d when corrected mortality be respectively 81.01%, 94.87%, in comparative example 7, corresponding insecticide efficiency is significantly reduced, and is said
After filler is added in the formula of the bright present invention, can effectively adsorb the effective insecticidal constituent in pulvis, and pine moth is also had
There are preferable adhesive force, therefore utilization rate height, the good disinsection effect of medicine.
Finally illustrate, only to illustrate technical scheme and unrestricted, this area is common for above example
Other modifications or equivalent that technical staff is made to technical scheme, without departing from technical solution of the present invention
Spirit and scope, all should cover in the middle of scope of the presently claimed invention.
Claims (7)
1. it is a kind of preventing and treating pine moth Microbial wettable powder, it is characterised in that including the raw material of following weight portion：Catharanthus roseus
30-40 parts, black false hellebore 22-36 parts, rhizome of Chinese monkshood 15-27 parts, pomelo peel 14-20 parts, muscardine powder 8-12 parts, chafer bacillus
Powder 4-10 parts, wetting agent 4-8 parts, dispersant 6-14 parts, disintegrant 5-10 parts and filler 50-60 parts.
2. it is according to claim 1 preventing and treating pine moth Microbial wettable powder, it is characterised in that：Including following weight
The raw material of part：35 parts of catharanthus roseus, 29 parts of black false hellebore, 21 parts of the rhizome of Chinese monkshood, 17 parts of pomelo peel, 10 parts of muscardine powder, chafer gemma bar
55 parts of 7 parts of bacterium powder end, 6 parts of wetting agent, 10 parts of dispersant, 7 parts of disintegrant and filler.
3. it is according to claim 1 and 2 preventing and treating pine moth Microbial wettable powder, it is characterised in that：The white deadlock
Effective content >=2 × 10 at bacterium powder end8cfu/g；Effective content >=2 × 10 of the chafer bacillus powder8cfu/g。
4. it is according to claim 1 and 2 preventing and treating pine moth Microbial wettable powder, it is characterised in that：
The one kind of the wetting agent in sodium lignin sulfonate, cetyl benzenesulfonic acid sodium and sulfo fatty acid ester；
The one kind of the disintegrant in ammonium sulfate, sodium sulphate and urea；
The one kind of the dispersant in maleic anhydride oligomers and fatty alcohol sulfate salt.
5. it is according to claim 1 and 2 preventing and treating pine moth Microbial wettable powder, it is characterised in that：Described filler
One kind in sepiolite, bentonite and diatomite.
6. it is a kind of as described in any one of claim 1-5 preventing and treating pine moth Microbial wettable powder preparation method, its
It is characterised by, comprises the following steps：
（1）Pretreatment：Black false hellebore root that is clean, being dried, catharanthus roseus herb, aconite root and fresh pomelo peel are selected, is crushed respectively
To 40-80 mesh, mixed-powder after mixing, is obtained, in terms of mixed-powder gross mass, the volume fraction for adding 12-18 times is 60-80% second
Alcoholic solution, ultrasonic extraction 4-8h, supersonic frequency 20-30KHz are collected extract, are concentrated into without alcohol taste, medicine is obtained final product after vacuum drying
Thing extract；
（2）Granulation：By step（1）In drug extract, wetting agent, dispersant and filler stir, then add white
Stiff bacterium powder end, chafer bacillus powder and disintegrant, are again stirring for and are crushed to 300-500 mesh, obtain final product product.
7. it is according to claim 6 preventing and treating pine moth Microbial wettable powder preparation method, it is characterised in that institute
State step（1）In, the temperature of ultrasonic extraction is 20-40 DEG C.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116035023A (en) * | 2023-02-03 | 2023-05-02 | 南通施壮化工有限公司 | Preparation method of indoxacarb insecticide |
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