CN102524271B - Cytisine and matrine insecticide water emulsion - Google Patents
Cytisine and matrine insecticide water emulsion Download PDFInfo
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- CN102524271B CN102524271B CN201110384514.8A CN201110384514A CN102524271B CN 102524271 B CN102524271 B CN 102524271B CN 201110384514 A CN201110384514 A CN 201110384514A CN 102524271 B CN102524271 B CN 102524271B
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- matrine
- extract
- sparteine
- cytisine
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Abstract
The invention relates to cytisine and matrine insecticide water emulsion, which belongs to the water emulsion obtained through compounding 15 to 20 volume percent of gorse cytisine extract liquid with 8 to 10 volume percent of matrine extract liquid, wherein 1ml of medicine liquid of gorse cytisine extract liquid equals to 2g of crude drugs, and 1ml of medicine liquid of matrine extract liquid equals to 2g of crude drugs. The two kinds of plant source alkaloid of the cytisine and the matrine are mixed, so biopesticide with the advantages of high efficiency, low toxicity and little environment pollution is obtained. Major ingredients such as the cytosine and the matrine are natural products extracted from plants, and both the cytisine and the matrine have the insecticidal action, so strong killing effects are realized on pests, the drug resistance is not easy to occur, no harmful chemical pesticide ingredients are contained, the toxicity on people and livestock is low, no residue is left, and the environment is not polluted.
Description
Technical field
The invention belongs to biological pesticide technical field, particularly relate to a kind of sparteine matrine disinsection aqueous emulsion.
Background technology
In state-owned thousands of years application medicinal plant experience, there is unique traditional Chinese medical theory, there is traditional Chinese herbal medicine compatibility theory, botanical pesticide is wide in variety, the plant extractss such as thick grass, argy wormwood, black false hellebore, tobacco, Dalmatian chrysanthemum and trifoliate jewelvine use for many years as insecticide, accumulated a lot of toxicitys, drug effect data, this provides rich material, theory and technical foundation for development of new biopesticide.
Chinese Plants source pesticide source is very extensive, has found out that nearly 1300 various plants contain desinsection, Fungicidal active substance.From current result of study, agriculture medicinal plant mainly concentrates in the plants such as Meliaceae, composite family, pulse family, Celastraceae, Euphorbiaceae, Labiatae, Magnoliaceae, Aristolochiaceae, polygonaceae, Oleaceae, Liliaceae, Curcurbitaceae, sedge family, Cruciferae.
Sparteine is the chemical composition in pulse family Thermopsis plant experiment of general alkaloid of Thermopsis, and molecular formula is C
11h
14n
2o, structure is as follows.
Only have now the report of a small amount of sparteine insecticidal activity, cabbage caterpillar, aphid, aleyrodid, diamond-back moth, leafhopper, plant hopper, twill leaf moth, beet webworm, mythimna separata, pine caterpillars, looper and low age cutworm etc. are had to certain control efficiency.But because the single dose quick-acting of botanical biological pesticide in producing is poor, thereby exist insect drug effect slowly, field efficacy is general, spray medicine often, the defect such as extension rate is lower.
Matrine is the chemical composition in traditional Chinese medicine leguminous plant Sophora alopecuroide, belongs to the quinolizine pyridine class in alkaloid, and molecular formula is C
15h
24n
2o, structure is as follows.
Verified at present: matrine has insecticidal action, the mechanism of action, for paralysis insect nerve center, makes polypide protein coagulating then, blocks polypide pore, and insect is choked to death.The existing many reports of matrine control agricultural and sanitary insect pest, have toxic action to various pests, as cabbage caterpillar, elm fleautiauxia armata black peach aphid, radish aphid, diamond-back moth, wheat aphid etc.
Sparteine and matrine are the quality raw materials compositions that can be used to make botanical biological pesticide.But because the single dose quick-acting of botanical biological pesticide in producing is poor, thereby exist insect drug effect slowly, field efficacy is poor, spray medicine often, the defect such as extension rate is lower.
Have no at present the complex preparation of sparteine and matrine.
Summary of the invention
Object of the present invention is just to provide a kind of sparteine matrine disinsection aqueous emulsion.
The present invention is achieved through the following technical solutions:
A kind of sparteine matrine disinsection aqueous emulsion, it is characterized in that by sparteine extract and the composite aqueous emulsion forming of matrine extract wherein the consumption of sparteine extract be 15%～20%, its every 1 milliliter of liquid is equivalent to 2 grams of crude drugs, the consumption of matrine extract is 8%～10%, its 1 every milliliter of liquid is equivalent to 2 grams of crude drugs, and above-mentioned consumption is in percent by volume;
Above-mentioned sparteine extract is suction filtration after adopting alcohol immersion extraction or ultrasonic extraction or alcohol reflux heating to extract the experiment of general alkaloid of Thermopsis after pulverizing, the concentrated rear gained of rotary evaporation;
Above-mentioned matrine extract is suction filtration after adopting alcohol immersion extraction or ultrasonic extraction or alcohol reflux heating to extract the Sophora alopecuroide after pulverizing, the concentrated rear gained of rotary evaporation;
Described alcohol immersion is extracted and is referred to that the ethanol that 10～15 times of employings, volume fraction are 60%～90% soaks 12～36 hours at normal temperatures;
Described ultrasonic extraction after referring to and adopting ethanol that 10～15 times, volume fraction are 60%～90% to dissolve again under 35～55 DEG C of temperature condition, ultrasonic extraction 30～60min under 70-100MHz;
The heating of described alcohol reflux extract refer to adopt ethanol that 10～15 times, volume fraction are 60%～90% to dissolve after refluxing extraction 1.5 hours under 50～70 DEG C of temperature condition again;
The auxiliary agent of above-mentioned aqueous emulsion is synergist, solvent and emulsifier;
Described synergist is castor oil and turpentine oil, and wherein castor oil adopts the castor-oil plant acetone of 10～15 times of amounts after pulverizing is infiltrated to solution 12～36 hours at normal temperatures, suction filtration, and rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains;
Described solvent is acetone;
Described emulsifier is one or more in agriculture breast 500, alkylbenzene and OP-10.
The present invention mixes sparteine and matrine two Plants source alkaloids, thereby obtains efficient, low toxicity and the little biopesticide of environmental pollution.The present invention's main component such as sparteine and matrine used is all the natural products extracting from plant, sparteine and matrine have insecticidal action, therefore insect is had to very strong killing action, be difficult for developing immunity to drugs, not containing any harmful chemical pesticide composition, to people and animals' low toxicity, do not have residual, free from environmental pollution.
Below sparteine matrine disinsection aqueous emulsion virulence test of the present invention:
This test is carried out in matrimony vine research institute of Ningxia Academy of Agri-Forestry Sciences laboratory, and test adopts infusion process, measures the tag virulence of compound to aphid.Be mixed with stock solution according to the compound method in embodiment 4, when use, according to pre-test result, storing solution water gradient dilution is become to 250,500,1000, the liquid of 2000 times, with micro intravenous drip device by the liquid drop of certain volume on the pronotary of aphid.Examination worm after drop is transferred to respectively in the culturing room that (28 ± 2) DEG C, relative moisture are 50%～75% and is cultivated.Every processing repeats for 3 times, and each dosage is processed 30 of examination worms.Then observe aphid mortality, calculate the lethality of each processing, the results are shown in Table 1.
The insecticidal effect of the different extension rates of table 1
Sparteine matrine disinsection aqueous emulsion field trial:
Experimental field be located at matrimony vine research institute of Ningxia Academy of Agri-Forestry Sciences experimental field, 2 years No. 1, peaceful Qis of life, experimental field management is strict, and water and fertilizer condition is good.1000 times of dilution processing of sparteine matrine disinsection aqueous emulsion are established in test.Take the dispenser of foliar spray method at 1 instar larvae initial stage of origination, evenly to squirt leave dual sides as degree.Respectively before dispenser, 1d, 3d, 5d, 7d after a medicine, after secondary medicine, 7d, 14d investigate 6 times altogether.When investigation every tree from East, West, South, North, different azimuth choose 5 branches, investigate the insect population number of aphid on the long tender tip of every branch 30cm.
Result of the test shows that 1000 times of sparteine matrine disinsection aqueous emulsions of the present invention have good control efficiency to Wolfberry Aphid, and after medicine, 3d preventive effect is the highest, and preventive effect reaches more than 90%.
Embodiment
Embodiment 1
The acquisition of 1 sparteine extract
First experiment of general alkaloid of Thermopsis was pulverized to 60 mesh sieves with cracker, then by raw material powder alcohol immersion, steep parameters is that volume fraction of ethanol is 90%, 10 times of ethanol consumptions, normal temperature, 24 hours.Then suction filtration, obtains after rotary evaporation is concentrated, and the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The acquisition of 2 matrine extracts
First Sophora alopecuroide is pulverized, crossed 60 mesh sieves, after the ethanol dissolving that is then 70% by raw material powder with 15 times, volume fraction, under 70 DEG C of temperature condition, refluxing extraction obtains for 1.5 hours again, and the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 16%, matrine extract 8%, castor oil 16%, turpentine oil 20%, acetone 30%, emulsifier 10%.
Wherein emulsifier adopts agriculture breast 500 (calcium dodecyl benzene sulfonates): alkylbenzene=1: the ratio preparation of 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant acetone of 15 times of amounts after pulverizing is infiltrated to solution 24 hours at normal temperatures, suction filtration, and rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains.
Embodiment 2
The acquisition of 1 sparteine extract
First experiment of general alkaloid of Thermopsis was pulverized to 60 mesh sieves with cracker, then the ethanol that is 60% by raw material powder with 15 times, volume fraction dissolves, and then temperature is under 35 DEG C of conditions, the ultrasonic extraction of 100MHz 30min, then suction filtration, after rotary evaporation is concentrated, obtain, the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The acquisition of 2 matrine extracts
First Sophora alopecuroide is pulverized, crossed 60 mesh sieves, then by raw material powder alcohol immersion, steep parameters is that volume fraction of ethanol is 60%, 15 times of ethanol consumptions, normal temperature, 36 hours.Then suction filtration, obtains after rotary evaporation is concentrated, and the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Mix and get final product according to the raw material of following percent by volume.
Sparteine extract 20%, matrine extract 10%, castor oil 10%, turpentine oil 25%, acetone 25%, emulsifier 10%.
Wherein emulsifier adopts the ratio preparation of agriculture breast 500 (calcium dodecyl benzene sulfonate): OP-10 (alkylphenol polyoxyethylene)=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant acetone of 15 times of amounts after pulverizing is infiltrated to solution 24 hours at normal temperatures, suction filtration, and rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains.
Embodiment 3
The acquisition of 1 sparteine extract
First experiment of general alkaloid of Thermopsis was pulverized to 60 mesh sieves with cracker, the ethanol that is then 90% by raw material powder with 10 times, volume fraction after dissolving again under 50 DEG C of temperature condition refluxing extraction within 3 hours, obtain, the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The acquisition of 2 matrine extracts
First Sophora alopecuroide is pulverized, crossed 60 mesh sieves, after the ethanol dissolving that raw material powder is 90% with 12 times, volume fraction, under 70 DEG C of temperature condition, refluxing extraction obtains for 1 hour again, and the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 15%, matrine extract 10%, castor oil 18%, turpentine oil 22%, acetone 27%, emulsifier 8%.
Wherein emulsifier adopts the ratio preparation of agriculture breast 500 (calcium dodecyl benzene sulfonate): OP-10 (alkylphenol polyoxyethylene)=1: 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant acetone of 10 times of amounts after pulverizing is infiltrated to solution 36 hours at normal temperatures, suction filtration, and rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains.
Embodiment 4
The acquisition of 1 sparteine extract
First experiment of general alkaloid of Thermopsis was pulverized to 60 mesh sieves with cracker, the ethanol that is then 90% by raw material powder with 10 times, volume fraction after dissolving again under 70 DEG C of temperature condition refluxing extraction within 1 hour, obtain, the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The acquisition of 2 matrine extracts
First Sophora alopecuroide is pulverized, crossed 60 mesh sieves, the ethanol dissolving that is then 80% by raw material powder with 12 times, volume fraction, and then temperature is under 55 DEG C of conditions, the ultrasonic extraction of 70MHz 60min, then suction filtration, after rotary evaporation is concentrated, obtain, the every 1 milliliter of liquid of this extract is equivalent to 2 grams of crude drugs.
The preparation of 3 sparteine matrine disinsection aqueous emulsions
Sparteine extract 18%, matrine extract 9%, castor oil 13%, turpentine oil 20%, acetone 30%, emulsifier 10%.
Wherein emulsifier adopts agriculture breast 500 (calcium dodecyl benzene sulfonates): alkylbenzene=1: the ratio preparation of 1 (volume ratio).
Above-mentioned castor oil adopts the castor-oil plant acetone of 10 times of amounts after pulverizing is infiltrated to solution 36 hours at normal temperatures, suction filtration, and rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains.The specific embodiment of the present invention is not limited to the mode that above-described embodiment provides.
Claims (2)
1. a sparteine matrine disinsection aqueous emulsion, it is characterized in that by sparteine extract and the composite aqueous emulsion forming of matrine extract, wherein the consumption of sparteine extract is 15%～20%, its every 1 milliliter of liquid is equivalent to 2 grams of crude drugs, the consumption of matrine extract is 8%～10%, its every 1 milliliter of liquid is equivalent to 2 grams of crude drugs, and above-mentioned consumption is in percent by volume; Wherein
Above-mentioned sparteine extract be after adopting ethanol that 10～15 times, volume fraction are 60%～90% to dissolve the experiment of general alkaloid of Thermopsis after pulverizing again under 35～55 DEG C of temperature condition, after the ethanol that under 70-100MHz, the ultrasonic extraction of ultrasonic extraction 30～60min or 10～15 times of employings, volume fraction are 60%～90% dissolves, under 50～70 DEG C of temperature condition, refluxing extraction is extracted rear suction filtration for 1～3 hour again, the concentrated rear gained of rotary evaporation;
Above-mentioned matrine extract be after adopting ethanol that 10～15 times, volume fraction are 60%～90% to dissolve the Sophora alopecuroide after pulverizing again under 35～55 DEG C of temperature condition, under 70-100MHz the ultrasonic extraction of ultrasonic extraction 30～60min or adopt ethanol that 10～15 times, volume fraction are 60%～90% to dissolve after refluxing extraction suction filtration after 1～3 hour under 50～70 DEG C of temperature condition again, gained after rotary evaporation is concentrated;
The auxiliary agent of described aqueous emulsion is synergist, solvent and emulsifier, and wherein said synergist is castor oil and turpentine oil, and described solvent is acetone, and described emulsifier is one or more in agriculture breast 500, alkylbenzene and OP-10.
2. according to sparteine matrine disinsection aqueous emulsion claimed in claim 1, it is characterized in that described castor oil employing infiltrates the acetone of 10～15 times of amounts of castor-oil plant after pulverizing at normal temperatures and separates 12～36 hours, suction filtration, rotary evaporation is concentrated into every milliliter of liquid and is equivalent to 2 grams of crude drugs and obtains.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110384514.8A CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110384514.8A CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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|---|---|
| CN102524271A CN102524271A (en) | 2012-07-04 |
| CN102524271B true CN102524271B (en) | 2014-12-10 |
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| CN201110384514.8A Expired - Fee Related CN102524271B (en) | 2011-11-28 | 2011-11-28 | Cytisine and matrine insecticide water emulsion |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107896839B (en) * | 2017-10-19 | 2019-11-12 | 广西壮族自治区金秀瑶族自治县科学技术情报研究所 | A kind of control method that illiciumverum is chrysomelid |
| CN109006844A (en) * | 2018-08-24 | 2018-12-18 | 广西田园生化股份有限公司 | A kind of agricultural insecticide composition of the derivative containing sparteine and its utilization |
| CN112335697A (en) * | 2020-10-23 | 2021-02-09 | 湖南顺新龙辰生物工程有限公司 | Plant protective agent and preparation method thereof |
Citations (2)
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|---|---|---|---|---|
| CN101199278A (en) * | 2006-12-14 | 2008-06-18 | 王国强 | Agricultural chemical composition containing sparteine |
| CN101624393A (en) * | 2009-08-04 | 2010-01-13 | 王答祺 | Method for extracting cytosine and thermopsine from thermopsis lanceolata seeds |
-
2011
- 2011-11-28 CN CN201110384514.8A patent/CN102524271B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199278A (en) * | 2006-12-14 | 2008-06-18 | 王国强 | Agricultural chemical composition containing sparteine |
| CN101624393A (en) * | 2009-08-04 | 2010-01-13 | 王答祺 | Method for extracting cytosine and thermopsine from thermopsis lanceolata seeds |
Non-Patent Citations (6)
| Title |
|---|
| 中药苦参中苦参碱类成分超声波提取工艺;邹姝姝 等;《重庆大学学报(自然科学版)》;20070731;第30卷(第7期);第130-133页 * |
| 披针叶黄华中金雀花碱类生物碱的提取工艺;黎萍 等;《华西药学杂志》;20071231;第22卷(第1期);第7-8页 * |
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| 邹姝姝 等.中药苦参中苦参碱类成分超声波提取工艺.《重庆大学学报(自然科学版)》.2007,第30卷(第7期),第130-133页. * |
| 黎萍 等.披针叶黄华中金雀花碱类生物碱的提取工艺.《华西药学杂志》.2007,第22卷(第1期),第7-8页. * |
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