CN107873741A - A kind of pharmaceutical composition for killing root-knot nematode - Google Patents
A kind of pharmaceutical composition for killing root-knot nematode Download PDFInfo
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- CN107873741A CN107873741A CN201711094608.5A CN201711094608A CN107873741A CN 107873741 A CN107873741 A CN 107873741A CN 201711094608 A CN201711094608 A CN 201711094608A CN 107873741 A CN107873741 A CN 107873741A
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- extract
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- pharmaceutical composition
- knot nematode
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
- A01N59/22—Arsenic
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/18—Euphorbiaceae [Spurge family], e.g. ricinus [castorbean]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of pharmaceutical composition for killing root-knot nematode, including pumkin seed P.E, arsenolite extract, Semen Pharbitidis extract, radish English extract, radish seed extract, root of langdu extract, tiger Lei extracts, Ran She le extract, banyan must extract and jervines.Heretofore described pharmaceutical composition being capable of targetedly eliminating root-knot nematodes, it is and without any side effects to the natural enemy of root-knot nematode, security is good, it is biodegradable good, pollution-free and penetrating odor, no environment toxic action, in animal body noresidue, the ecological balance is not destroyed, crop growth environment is influenceed small；With preferable permeability and wettability, the central nervous system of polypide is disturbed, suppresses worm's ovum synthesis development, is not susceptible to drug resistance；Quick-acting is good, is adapted to the wide popularization and application in terms of control of insect.
Description
Technical field
The invention belongs to agricultural technology development field, in particular it relates to a kind of pharmaceutical composition for killing root-knot nematode.
Background technology
Chemical agent plays an important role in terms of crop pest control, but the residual of medicine causes nature
The ecosystem is worsening, and this has turned into international common concern and key subjects urgently to be resolved hurrily.Since China, twentieth century
Because single use chemical pesticide causes agricultural product harmful element exceeded, health and the existence of the people are directly endangered, therefore, state
Family pays much attention to and formulated《Medium-term and long-term scientific and technical development program outline》, it is therefore intended that encourage research and development environment-friendly type fertilizer, agriculture
Medicine." seeking speed " psychology of current peasant is incompatible, and most farmers are still selected using high poison, the chemical pesticide of high residue, this
Chemical pesticide can use only for crop, i.e., kill each class pest for certain crop simultaneously.But this chemical pesticide exists
Today of green and environmental protection is advocated, a variety of high poisons and high residue chemicals are limited the quantity or stopped production, further, since insect
The difference of species, cause each class pest to occur larger difference in terms of the resistance to the action of a drug and drug resistance, so just make killing for chemical agent
Easily there is resistance insect in worm effect, therefore propose to prevent specific insect from just seeming for the insect-proof agent of different insects to have very much must
Will, but yet there are no corresponding report.
The content of the invention
It is good that the technical problems to be solved by the invention are to provide a kind of security, biodegradable good, pollution-free and excitant
Smell, no environment toxic action, in animal body noresidue, specific aim kill the pharmaceutical composition of root-knot nematode.
Technical scheme is used by the present invention solves above-mentioned technical problem：A kind of drug regimen for killing root-knot nematode
Thing, including pumkin seed P.E, arsenolite extract, Semen Pharbitidis extract, radish English extract, radish seed extract, the root of langdu carry
Take thing, tiger Lei extracts, Ran She le extract, banyan must extract and jervine.
In order to which the present invention is better achieved, further, contain the south equivalent to 5-15g pumpkin seeds medicinal materials per 100ml solution
Melon seeds extract, the arsenolite extract of 5-15g arsenolite medicinal materials, the Semen Pharbitidis extract of 5-15g kaladana's medicinal material, 5-15g radish
The radish English extract of English medicinal material, the radish seed extract of 5-15g radish seed medicinal materials, 5-15g root of langdu medicinal materials root of langdu extract,
The tiger Lei extracts of 5-15g tiger Lei medicinal materials, the Ran She le extract of 5-15g Ran She le medicinal materials, 5-15g banyans must medicinal materials
Banyan must extract, pumkin seed P.E, arsenolite extract, Semen Pharbitidis extract, radish English extract, radish seed extract,
Root of langdu extract, tiger Lei extracts, Ran She le extract, the usage ratio of banyan palpus extract medicinal material are 1：1：1：2：4：2：
3：2：5.
In order to which the present invention is better achieved, further, the dosage of the jervine is 0.005g~5.0g/100ml.
In order to which the present invention is better achieved, further, the dosage of the jervine is 0.05g~1.0g/100ml.
In order to which the present invention is better achieved, further, described pharmaceutical composition formulation is powder pin or freezed.
The preparation method of the pharmaceutical composition of above-mentioned killing root-knot nematode is prepared, is comprised the steps：
(1) pharmaceutical composition raw medicinal material pumpkin seeds 50g, arsenolite 50g, the kaladana 50g of root-knot nematode, radish English are killed
100g, radish seed 200g, root of langdu 100g, tiger Lei 150g, Ran She le 100g, banyan must 250g, jervine 15g；
(2) pumpkin seeds, arsenolite, kaladana add water to cook secondary, 2 hours every time, collecting decoction, add ethanol filtering and concentrating,
Add 3 times of amount water, be sufficiently stirred, refrigerate 48 hours, filtration, it is about 1.15 that filtrate decompression, which is concentrated into relative density, and decoction is standby；
(3) radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook secondary, 1 hour every time, merge
Decocting liquid, filtration, filtrate is concentrated, is let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, filtrate decompression is concentrated into phase
It is 1.20 to density, it is 70% to add ethanol to make containing amount of alcohol, stands overnight, filters, it is 1.30 that filtrate, which is concentrated into relative density, is added
3 times of amount water, are sufficiently stirred, refrigerate 48 hours, and filtration, it is 1.15 that filtrate, which is concentrated into relative density,
(4) merge with above-mentioned all decoctions, add 2 times of amount water, be sufficiently stirred, refrigerate 24 hours, filtration, filtrate adds black false hellebore
Alkali and deionized water water are configured to 1000ml solution, stir evenly, the relative density when relative density of above-mentioned each filtrate is 80 DEG C；
(5) solution ph is adjusted to 6.5-8.5 with sodium hydroxide solution；
(6) filter, it is filling, sterilizing, produce.
The present invention compared with prior art, has advantages below and beneficial effect：
(1) heretofore described pharmaceutical composition being capable of targetedly eliminating root-knot nematodes, and to the day of root-knot nematode
Oppose without any side effects, security is good, biodegradable good, pollution-free and penetrating odor, no environment toxic action, in animal
Internal noresidue, does not destroy the ecological balance, crop growth environment is influenceed small；
(2) low raw-material cost used in heretofore described pharmaceutical composition, there is preferable permeability and wet
Lubricant nature, easily breaks through root-knot nematode wax coat, and depth destroys the epidermal structure of polypide and internal protein, makes root-knot nematode not
Drug resistance easily occurs；
(3) heretofore described pharmaceutical composition can interfere with the central nervous system of polypide, suppress worm's ovum synthesis hair
Educate；
(4) heretofore described pharmaceutical composition quick-acting is good, and after spray, root-knot nematode stopped in 20~40 minutes
Food is dead, is adapted to the wide popularization and application in terms of control of insect.
Embodiment
For the purpose of the present invention, process conditions and advantage effect is more clearly understood, with reference to following embodiment, to this
Invention is described in further detail, but the implementation of the present invention is not limited to this, is not departing from the above-mentioned technological thought feelings of the present invention
Under condition, according to ordinary skill knowledge and customary means, various replacements and change are made, the model of the present invention all should be included in
In enclosing, specific implementation example described herein is not intended to limit the present invention only to explain the present invention.
Embodiment 1：
The present embodiment provide it is a kind of kill root-knot nematode pharmaceutical composition, including pumkin seed P.E, arsenolite extract,
Semen Pharbitidis extract, radish English extract, radish seed extract, root of langdu extract, tiger Lei extracts, Ran She le extract,
Banyan palpus extract and jervine, its component are as follows：
Its preparation method：Pumpkin seeds, arsenolite, kaladana add water to cook secondary, 2 hours every time, collecting decoction, add ethanol mistake
Filter concentration, add 3 times of amount water, be sufficiently stirred, refrigerate 48 hours, filtration, it is about 1.15 that filtrate decompression, which is concentrated into relative density, decoction
It is standby；Radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook it is secondary, 1 hour every time, collecting decoction,
Filtration, filtrate is concentrated, is let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, filtrate decompression is concentrated into relatively close
Spend for 1.20, it is 70% to add ethanol to make containing amount of alcohol, stands overnight, filters, and filtrate is concentrated into relative density as 1.30, adds 3 times
Water is measured, is sufficiently stirred, is refrigerated 48 hours, filtration, filtrate is concentrated into relative density as 1.15；Merge with above-mentioned all decoctions, add 2
Amount water again, is sufficiently stirred, and refrigerates 24 hours, and filtration, filtrate adds jervine and deionized water water is configured to 1000ml solution, stirs
It is even, the relative density when relative density of above-mentioned each filtrate is 80 DEG C；Solution ph is adjusted to 6.5- with sodium hydroxide solution
8.5；Filtering, it is filling, sterilizing, produce.
Wherein, the process for adding ethanol filtering and concentrating is first to filter to get filtrate, and filtrate decompression is concentrated into relative density as 1.27
~1.30, let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, and decompression filtrate recycling ethanol is simultaneously concentrated into relative
Density is 1.30.Filtrate is concentrated into relative density as 1.25~1.30.The mass percent of the sodium hydroxide solution used is
20%.Solution uses filtering with microporous membrane.
Embodiment 2：
The present embodiment verifies that the pharmaceutical composition insecticide common with market is compared, to the eradicative rate of root-knot nematode.
Choose insecticide common on the market：Pale purple purple spore bacterium aqua, nematode killing agent, the agent of special efficacy root line, thiamethoxam disinsection agent.
It is grouped according to different insecticides, every group prepares in worm's ovum, larva, the root knot of going out of adult three phases respectively
Each 1000 of nematode, then uses insecticide respectively to the root-knot nematode in each stage, tests killing for every group of insecticide
Rate, it is as shown in the table：
Killing effect of the insecticide of table one to root-knot nematode
As seen from the above table, each group insecticide is to larval root-knot nematode killing rate highest, and the insecticidal effect of this product is most
To be excellent, insect killing effect is notable, and the worm's ovum for being most difficult to kill also has preferable insect killing effect.
Embodiment 3：
The present embodiment verifies eradicative rate of the pharmaceutical composition to various insects.
Choose chafer, beetle, leafhopper, heart-eating worm, pear sucker, scale insect, volume noctuid, mole cricket, fore-telling mud, root-knot nematode
Each 10000, and be grouped respectively, the decoction of same concentrations is made in the medicine being prepared using embodiment 1, respectively to every group
Insect is sprayed, and after 1 hour, checks the eradicative rate of various insects, as a result as shown in the table：
Insect killing effect of the medicine of table two to various insects
| Group | Eradicative rate (%) |
| Root-knot nematode | 99.96 |
| Chafer | 10.32 |
| Beetle | 9.65 |
| Leafhopper | 8.75 |
| Heart-eating worm | 1.02 |
| Pear sucker | 0 |
| Scale insect | 0 |
| Roll up noctuid | 1.01 |
| Mole cricket | 0 |
| Foretell mud | 1.24 |
As seen from the above table, the medicine that embodiment 1 is prepared, only there is preferable insect killing effect to root-knot nematode, to other
The insect killing effect of insect is very limited, and thus its specific aim of provable this product is stronger, is only capable of using killing for root-knot nematode.
Claims (5)
1. a kind of pharmaceutical composition for killing root-knot nematode, it is characterised in that including pumkin seed P.E, arsenolite extract, lead
Ox seed extract, radish English extract, radish seed extract, root of langdu extract, tiger Lei extracts, Ran She le extract, banyan
Tree must extract and jervine.
2. a kind of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that per 100ml solution
Pumkin seed P.E containing equivalent to 5-15g pumpkin seeds medicinal materials, the arsenolite extract of 5-15g arsenolite medicinal materials, 5-15g kaladana's medicine
The Semen Pharbitidis extract of material, the radish English extract of 5-15g radish English medicinal materials, 5-15g radish seed medicinal materials radish seed extract,
The root of langdu extract of 5-15g root of langdu medicinal materials, the tiger Lei extracts of 5-15g tiger Lei medicinal materials, the boa of 5-15g Ran She le medicinal materials
Le extract, 5-15g banyans must medicinal material banyan must extract, pumkin seed P.E, arsenolite extract, Semen Pharbitidis extract,
Radish English extract, radish seed extract, root of langdu extract, tiger Lei extracts, Ran She le extract, banyan must extract medicines
The usage ratio of material is 1：1：1：2：4：2：3：2：5.
3. a kind of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that the jervine
Dosage is 0.005g~5.0g/100ml.
4. a kind of pharmaceutical composition for killing root-knot nematode according to claim 3, it is characterised in that the jervine
Dosage is 0.05g~1.0g/100ml.
A kind of 5. pharmaceutical composition of killing root-knot nematode according to claim 1 ~ 4 any one, it is characterised in that institute
Medicine composition dosage form is stated as powder pin or lyophilized.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094608.5A CN107873741A (en) | 2017-11-09 | 2017-11-09 | A kind of pharmaceutical composition for killing root-knot nematode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711094608.5A CN107873741A (en) | 2017-11-09 | 2017-11-09 | A kind of pharmaceutical composition for killing root-knot nematode |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107873741A true CN107873741A (en) | 2018-04-06 |
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ID=61779509
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711094608.5A Pending CN107873741A (en) | 2017-11-09 | 2017-11-09 | A kind of pharmaceutical composition for killing root-knot nematode |
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| CN (1) | CN107873741A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110352995A (en) * | 2019-08-07 | 2019-10-22 | 广西壮族自治区农业科学院 | A kind of plant medical agent for preventing and treating ginger root knot nematode disease |
-
2017
- 2017-11-09 CN CN201711094608.5A patent/CN107873741A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110352995A (en) * | 2019-08-07 | 2019-10-22 | 广西壮族自治区农业科学院 | A kind of plant medical agent for preventing and treating ginger root knot nematode disease |
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Application publication date: 20180406 |
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