CN107788017A - A kind of preparation method for killing root-knot nematode pharmaceutical composition - Google Patents

A kind of preparation method for killing root-knot nematode pharmaceutical composition Download PDF

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Publication number
CN107788017A
CN107788017A CN201711094592.8A CN201711094592A CN107788017A CN 107788017 A CN107788017 A CN 107788017A CN 201711094592 A CN201711094592 A CN 201711094592A CN 107788017 A CN107788017 A CN 107788017A
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filtrate
root
concentrated
pharmaceutical composition
relative density
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CN201711094592.8A
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Chinese (zh)
Inventor
黄伟
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Chengdu Aibike Biotechnology Co Ltd
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Chengdu Aibike Biotechnology Co Ltd
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Priority to CN201711094592.8A priority Critical patent/CN107788017A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/22—Arsenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Inorganic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of preparation method for the pharmaceutical composition for killing root-knot nematode, comprise the steps：1）Prepare raw material；2）Pumpkin seeds, arsenolite, kaladana add water to cook secondary, and decoction is standby；3）Radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook secondary, filtration, and filtrate is concentrated into relative density as 1.15；4）Merge decoction；5）Adjust solution ph；6）Filtering, it is filling, sterilizing, produce.Heretofore described pharmaceutical composition can targetedly eliminating root-knot nematodes, security is good, biodegradable good, pollution-free and penetrating odor, no environment toxic action, noresidue in animal body, does not destroy the ecological balance, crop growth environment is influenceed small；With preferable permeability and wettability, the central nervous system of polypide is disturbed, suppresses worm's ovum synthesis development, is not susceptible to drug resistance；Quick-acting is good, is adapted to the wide popularization and application in terms of control of insect.

Description

A kind of preparation method for killing root-knot nematode pharmaceutical composition
Technical field
The invention belongs to agricultural technology development field, in particular it relates to a kind of system for killing root-knot nematode pharmaceutical composition Preparation Method.
Background technology
Chemical agent plays an important role in terms of crop pest control, but the residual of medicine causes nature The ecosystem is worsening, and this has turned into international common concern and key subjects urgently to be resolved hurrily.Since China, twentieth century Because single use chemical pesticide causes agricultural product harmful element exceeded, health and the existence of the people are directly endangered, therefore, state Family pays much attention to and formulated《Medium-term and long-term scientific and technical development program outline》, it is therefore intended that encourage research and development environment-friendly type fertilizer, agriculture Medicine." seeking speed " psychology of current peasant is incompatible, and most farmers are still selected using high poison, the chemical pesticide of high residue, this Chemical pesticide can use only for crop, i.e., kill each class pest for certain crop simultaneously.But this chemical pesticide exists Today of green and environmental protection is advocated, a variety of high poisons and high residue chemicals are limited the quantity or stopped production, further, since insect The difference of species, cause each class pest to occur larger difference in terms of the resistance to the action of a drug and drug resistance, so just make killing for chemical agent Easily there is resistance insect in worm effect, therefore propose to prevent specific insect from just seeming for the insect-proof agent of different insects to have very much must Will, but yet there are no corresponding report.
The content of the invention
The technical problems to be solved by the invention are to provide that a kind of preparation process is simple, and manufacturing cost is cheap, process Maturation, the preparation method for the killing root-knot nematode pharmaceutical composition that can produce in enormous quantities.
Technical scheme is used by the present invention solves above-mentioned technical problem：A kind of pharmaceutical composition for killing root-knot nematode Preparation method, comprise the steps：
(1) pharmaceutical composition raw medicinal material pumpkin seeds 50g, arsenolite 50g, the kaladana 50g of root-knot nematode, radish English are killed 100g, radish seed 200g, root of langdu 100g, tiger Lei 150g, Ran She le 100g, banyan must 250g, jervine 15g；
(2) pumpkin seeds, arsenolite, kaladana add water to cook secondary, 2 hours every time, collecting decoction, add ethanol filtering and concentrating, Add 3 times of amount water, be sufficiently stirred, refrigerate 48 hours, filtration, it is about 1.15 that filtrate decompression, which is concentrated into relative density, and decoction is standby；
(3) radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook secondary, 1 hour every time, merge Decocting liquid, filtration, filtrate is concentrated, is let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, filtrate decompression is concentrated into phase It is 1.20 to density, it is 70% to add ethanol to make containing amount of alcohol, stands overnight, filters, it is 1.30 that filtrate, which is concentrated into relative density, is added 3 times of amount water, are sufficiently stirred, refrigerate 48 hours, and filtration, it is 1.15 that filtrate, which is concentrated into relative density,
(4) merge with above-mentioned all decoctions, add 2 times of amount water, be sufficiently stirred, refrigerate 24 hours, filtration, filtrate adds black false hellebore Alkali and deionized water water are configured to 1000ml solution, stir evenly, the relative density when relative density of above-mentioned each filtrate is 80 DEG C；
(5) solution ph is adjusted to 6.5-8.5 with sodium hydroxide solution；
(6) filter, it is filling, sterilizing, produce.
In order to which the method for the present invention is better achieved, further, in the step (2), add the mistake of ethanol filtering and concentrating Cheng Wei, first filter to get filtrate, filtrate decompression is concentrated into relative density as 1.27~1.30, lets cool, add ethanol make be containing amount of alcohol 65%, stand overnight, filter, decompression filtrate recycling ethanol is simultaneously concentrated into relative density as 1.30.
In order to which the method for the present invention is better achieved, further, in the step (3), filtrate is concentrated into relative density For 1.25~1.30.
In order to which the method for the present invention is better achieved, further, in the step (5), the sodium hydroxide solution that uses Mass percent be 20%.
In order to which the method for the present invention is better achieved, further, in the step (6), solution uses miillpore filter mistake Filter.
The present invention compared with prior art, has with advantage and beneficial effect：
(1) it is good can to prepare security for preparation method of the present invention, biodegradable good, pollution-free and penetrating odor, Without environment toxic action, noresidue in animal body, the ecological balance is not destroyed, crop growth environment is influenceed small；
(2) preparation method of the present invention, preparation process is simple, and manufacturing cost is cheap, the pharmaceutical composition being prepared With preferable permeability and wettability, root-knot nematode wax coat is easily broken through, depth destroys the epidermal structure and in vivo of polypide Protein, root-knot nematode is not susceptible to drug resistance；
(4) preparation method of the present invention can improve the quick-acting of eliminating root-knot nematodes pharmaceutical composition, the medicine group When compound acts on root-knot nematode, root-knot nematode can be made to stop eating in 20~40 minutes death；
(5) its mature preparation process of preparation method of the present invention, can be used for producing eliminating root-knot nematodes in batches Pharmaceutical composition, it is adapted to the wide popularization and application in terms of control of insect.
Embodiment
For the purpose of the present invention, process conditions and advantage effect is more clearly understood, with reference to following embodiment, to this Invention is described in further detail, but the implementation of the present invention is not limited to this, is not departing from the above-mentioned technological thought feelings of the present invention Under condition, according to ordinary skill knowledge and customary means, various replacements and change are made, the model of the present invention all should be included in In enclosing, specific implementation example described herein is not intended to limit the present invention only to explain the present invention.
For the purpose of the present invention, process conditions and advantage effect is more clearly understood, with reference to following embodiment, to this Invention is described in further detail, but the implementation of the present invention is not limited to this, is not departing from the above-mentioned technological thought feelings of the present invention Under condition, according to ordinary skill knowledge and customary means, various replacements and change are made, the model of the present invention all should be included in In enclosing, specific implementation example described herein is not intended to limit the present invention only to explain the present invention.
Embodiment 1：
The present embodiment provide it is a kind of kill root-knot nematode pharmaceutical composition, including pumkin seed P.E, arsenolite extract, Semen Pharbitidis extract, radish English extract, radish seed extract, root of langdu extract, tiger Lei extracts, Ran She le extract, Banyan palpus extract and jervine, its component are as follows：
Its preparation method：Pumpkin seeds, arsenolite, kaladana add water to cook secondary, 2 hours every time, collecting decoction, add ethanol mistake Filter concentration, add 3 times of amount water, be sufficiently stirred, refrigerate 48 hours, filtration, it is about 1.15 that filtrate decompression, which is concentrated into relative density, decoction It is standby；Radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook it is secondary, 1 hour every time, collecting decoction, Filtration, filtrate is concentrated, is let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, filtrate decompression is concentrated into relatively close Spend for 1.20, it is 70% to add ethanol to make containing amount of alcohol, stands overnight, filters, and filtrate is concentrated into relative density as 1.30, adds 3 times Water is measured, is sufficiently stirred, is refrigerated 48 hours, filtration, filtrate is concentrated into relative density as 1.15；Merge with above-mentioned all decoctions, add 2 Amount water again, is sufficiently stirred, and refrigerates 24 hours, and filtration, filtrate adds jervine and deionized water water is configured to 1000ml solution, stirs It is even, the relative density when relative density of above-mentioned each filtrate is 80 DEG C；Solution ph is adjusted to 6.5- with sodium hydroxide solution 8.5；Filtering, it is filling, sterilizing, produce.
Wherein, the process for adding ethanol filtering and concentrating is first to filter to get filtrate, and filtrate decompression is concentrated into relative density as 1.27 ~1.30, let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, and decompression filtrate recycling ethanol is simultaneously concentrated into relative Density is 1.30.Filtrate is concentrated into relative density as 1.25~1.30.The mass percent of the sodium hydroxide solution used is 20%.Solution uses filtering with microporous membrane.
Embodiment 2：
The present embodiment verifies that the pharmaceutical composition insecticide common with market is compared, to the eradicative rate of root-knot nematode.
Choose insecticide common on the market：Pale purple purple spore bacterium aqua, nematode killing agent, the agent of special efficacy root line, thiamethoxam disinsection agent.
It is grouped according to different insecticides, every group prepares in worm's ovum, larva, the root knot of going out of adult three phases respectively Each 1000 of nematode, then uses insecticide respectively to the root-knot nematode in each stage, tests killing for every group of insecticide Rate, it is as shown in the table：
Killing effect of the insecticide of table one to root-knot nematode
As seen from the above table, each group insecticide is to larval root-knot nematode killing rate highest, and the insecticidal effect of this product is most To be excellent, insect killing effect is notable, and the worm's ovum for being most difficult to kill also has preferable insect killing effect.
Embodiment 3：
The present embodiment verifies eradicative rate of the pharmaceutical composition to various insects.
Choose chafer, beetle, leafhopper, heart-eating worm, pear sucker, scale insect, volume noctuid, mole cricket, fore-telling mud, root-knot nematode Each 10000, and be grouped respectively, the decoction of same concentrations is made in the medicine being prepared using embodiment 1, respectively to every group Insect is sprayed, and after 1 hour, checks the eradicative rate of various insects, as a result as shown in the table：
Insect killing effect of the medicine of table two to various insects
Group Eradicative rate (%)
Root-knot nematode 99.96
Chafer 10.32
Beetle 9.65
Leafhopper 8.75
Heart-eating worm 1.02
Pear sucker 0
Scale insect 0
Roll up noctuid 1.01
Mole cricket 0
Foretell mud 1.24
As seen from the above table, the medicine that embodiment 1 is prepared, only there is preferable insect killing effect to root-knot nematode, to other The insect killing effect of insect is very limited, and thus its specific aim of provable this product is stronger, is only capable of using killing for root-knot nematode.

Claims (5)

  1. A kind of 1. preparation method for the pharmaceutical composition for killing root-knot nematode, it is characterised in that：Comprise the steps：
    （1）Kill pharmaceutical composition raw medicinal material pumpkin seeds 50g, arsenolite 50g, the kaladana 50g of root-knot nematode, radish English 100g, radish seed 200g, root of langdu 100g, tiger Lei 150g, Ran She le 100g, banyan must 250g, jervine 15g；
    （2）Pumpkin seeds, arsenolite, kaladana add water to cook secondary, 2 hours every time, collecting decoction, add ethanol filtering and concentrating, add 3 times Water is measured, is sufficiently stirred, is refrigerated 48 hours, filtration, it is about 1.15 that filtrate decompression, which is concentrated into relative density, and decoction is standby；
    （3）Radish English, radish seed, the root of langdu, tiger Lei, Ran She le, banyan must add water to cook it is secondary, 1 hour every time, merge decoct Liquid, filtration, filtrate is concentrated, is let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, filtrate decompression is concentrated into relatively Density is 1.20, and it is 70% to add ethanol to make containing amount of alcohol, stands overnight, filters, it is 1.30 that filtrate, which is concentrated into relative density, adds 3 Amount water again, is sufficiently stirred, and refrigerates 48 hours, filtration, and filtrate is concentrated into relative density as 1.15；
    （4）Merge with above-mentioned all decoctions, add 2 times amount water, be sufficiently stirred, refrigerate 24 hours, filtration, filtrate add jervine and Deionized water water is configured to 1000ml solution, stirs evenly, the relative density when relative density of above-mentioned each filtrate is 80 DEG C；
    （5）Solution ph is adjusted to 6.5-8.5 with sodium hydroxide solution；
    （6）Filtering, it is filling, sterilizing, produce.
  2. A kind of 2. preparation method of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that institute State step（2）In, the process for adding ethanol filtering and concentrating is first to filter to get filtrate, and filtrate decompression is concentrated into relative density as 1.27 ~1.30, let cool, it is 65% to add ethanol to make containing amount of alcohol, stands overnight, filters, and decompression filtrate recycling ethanol is simultaneously concentrated into relative Density is 1.30.
  3. A kind of 3. preparation method of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that institute State step（3）In, filtrate is concentrated into relative density as 1.25~1.30.
  4. A kind of 4. preparation method of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that institute State step（5）In, the mass percent of the sodium hydroxide solution used is 20%.
  5. A kind of 5. preparation method of pharmaceutical composition for killing root-knot nematode according to claim 1, it is characterised in that institute State step（6）In, solution uses filtering with microporous membrane.
CN201711094592.8A 2017-11-09 2017-11-09 A kind of preparation method for killing root-knot nematode pharmaceutical composition Pending CN107788017A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711094592.8A CN107788017A (en) 2017-11-09 2017-11-09 A kind of preparation method for killing root-knot nematode pharmaceutical composition

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711094592.8A CN107788017A (en) 2017-11-09 2017-11-09 A kind of preparation method for killing root-knot nematode pharmaceutical composition

Publications (1)

Publication Number Publication Date
CN107788017A true CN107788017A (en) 2018-03-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
CN (1) CN107788017A (en)

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