CN110352995A - A kind of plant medical agent for preventing and treating ginger root knot nematode disease - Google Patents
A kind of plant medical agent for preventing and treating ginger root knot nematode disease Download PDFInfo
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 23
- 229940102465 ginger root Drugs 0.000 title claims abstract description 14
- 239000001841 zingiber officinale Substances 0.000 title claims abstract description 14
- 235000006886 Zingiber officinale Nutrition 0.000 claims abstract description 32
- 235000008397 ginger Nutrition 0.000 claims abstract description 32
- 239000002994 raw material Substances 0.000 claims abstract description 18
- 229940080817 rotenone Drugs 0.000 claims abstract description 13
- JUVIOZPCNVVQFO-UHFFFAOYSA-N rotenone Natural products O1C2=C3CC(C(C)=C)OC3=CC=C2C(=O)C2C1COC1=C2C=C(OC)C(OC)=C1 JUVIOZPCNVVQFO-UHFFFAOYSA-N 0.000 claims abstract description 13
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- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims abstract description 12
- 244000237986 Melia azadirachta Species 0.000 claims abstract description 12
- 244000269722 Thea sinensis Species 0.000 claims abstract description 12
- 244000089698 Zanthoxylum simulans Species 0.000 claims abstract description 12
- 229930014456 matrine Natural products 0.000 claims abstract description 12
- 240000002234 Allium sativum Species 0.000 claims abstract description 11
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- 239000000843 powder Substances 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 11
- 238000010564 aerobic fermentation Methods 0.000 claims description 10
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- 239000003208 petroleum Substances 0.000 claims description 8
- 230000002265 prevention Effects 0.000 claims description 8
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- 238000000034 method Methods 0.000 claims description 4
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- 230000010534 mechanism of action Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 244000273928 Zingiber officinale Species 0.000 abstract 1
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- 230000007423 decrease Effects 0.000 description 6
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- 239000000575 pesticide Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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Abstract
The present invention relates to plant medical agent technical fields, more particularly to a kind of plant medical agent for preventing and treating ginger root knot nematode disease, the raw material of the plant medical agent is composed of the following components by weight: 8-16 parts of tea seed cake, 7-21 parts of ginger, 8-20 parts of garlic, 15-25 parts of rotenone, 10-16 parts of banyan root, 7-11 parts of tree-of-heaven, 6-16 parts of black false hellebore, 16-24 parts of matrine, 10-16 parts of Tobacco Leaf, 15-25 parts of radix gentianae, 10-16 parts of Chinese prickly ash, 20-24 parts of margosa, 10-20 parts of betel nut and native 5-7 parts of ash.It is rich in a variety of insecticidal constituents to plant medical agent of the invention, mutually acts synergistically between each effective component, effectively prevents and kill off root-knot nematode by the different mechanism of action, significantly improve vermins-proof effect；Also rich in nutrient needed for plant growth, plant robust growth can be promoted, improve crop disease-resistant ability.
Description
Technical field
The present invention relates to plant medical agent technical fields, and in particular to a kind of plant medical agent for preventing and treating ginger root knot nematode disease.
Background technique
Ginger root knot nematode disease is commonly called as ginger bark favus, ginger a red-spotted lizard, be visually see less than nematodiasis, mostly occur on ground
On lower stem block, it can fall ill in ginger seedling stage to Adult plant, endanger meat and root system in ginger block epidermis, make ginger fibrous root and meat
Root is thicker to shorten, and fleshy root generates knob disease, and the knob of wrinkle protrusion occurs in female ginger base portion, with ginger block branch, table
Face forms protrusion knob and with crackle spot.Since root is aggrieved, ginger absorbing function is affected, and grows plant
Slowly, the ill symptoms such as leaf is small, stem is short.In recent years, with the expansion of ginger continuous cropping and cultivated area, the ginger root-knot nematode that makes
Disease becomes the second largest ginger diseases for being only second to bacterial wilt of ginger, seriously affects ginger in the trend for aggravating to cause harm and expand
High-quality, high yield, affects greatly economic benefit.
Currently, chemical prevention is a kind of most important control measure in terms of agricultural insect pests control, but use chemical pesticide
It when pest control, is easy to generate higher pesticide residue in crops, the health of people can be impacted；In addition exist
After spraying chemical pesticide, there is part pesticide that can drop onto soil, and then enter underground pollution underground water source, aggravate environmental pollution.
The information disclosed in the background technology section is intended only to increase the understanding to general background of the invention, without answering
When being considered as recognizing or imply that the information constitutes the prior art already known to those of ordinary skill in the art in any form.
Summary of the invention
To solve the above problems, the present invention provides a kind of plant medical agent for preventing and treating ginger root knot nematode disease, it can be effective
Ginger root knot nematode disease is prevented and treated, no pollution to the environment is a kind of green medicament of environment-friendly type.
To achieve the above object, the present invention provides the following technical scheme that
A kind of plant medical agent for preventing and treating ginger root knot nematode disease, the raw material of the plant medical agent is by weight by with the following group
Be grouped as: 8-16 parts of tea seed cake, 7-21 parts of ginger, 8-20 parts of garlic, 15-25 parts of rotenone, 10-16 parts of banyan root, 7-11 parts of tree-of-heaven,
6-16 parts of black false hellebore, 16-24 parts of matrine, 10-16 parts of Tobacco Leaf, 15-25 parts of radix gentianae, 10-16 parts of Chinese prickly ash, margosa 20-24
Part, 10-20 parts of betel nut and native 5-7 parts of ash.
Preferably, the raw material of the plant medical agent is composed of the following components by weight: 12 parts of tea seed cake, 14 parts of ginger,
14 parts of garlic, 20 parts of rotenone, 13 parts of banyan root, 9 parts of tree-of-heaven, 11 parts of black false hellebore, 20 parts of matrine, 13 parts of Tobacco Leaf, radix gentianae
6 parts of 20 parts, 13 parts of Chinese prickly ash, 22 parts of margosa, 15 parts of betel nut and soil ash.
The present invention also provides the preparation method of the prevention and treatment ginger root knot nematode disease plant medical agent, the plant medical agents
Preparation method the following steps are included:
S1. meter weighs each raw material component by weight ratio, is 5-7wt% by each raw material component drying to moisture content,
It crushed 45-60 mesh, obtain traditional Chinese medicine powder；
S2. extractant will be added in traditional Chinese medicine powder, be added in extraction kettle carry out CO after mixing2Supercritical extract obtains
Extract；Extract is centrifugated and removes impurity, recycling extractant obtains effective component of chinese medicine；
S3. microbial bacterial agent will be added in effective component of chinese medicine, after mixing first carry out 10-16d aerobic fermentation, then into
The anaerobic fermentation of row 25-30d filters after fermentation, obtains primary fermentation object；
S4. primary fermentation object is placed in a greenhouse and continues the 45-60d that ferments, filtered after fermentation, obtain plant medical agent.
Preferably, the mass ratio of traditional Chinese medicine powder described in S2 and extractant is 1:0.04-0.08；The extractant is petroleum
Ether.
Preferably, CO described in S22The pressure of supercritical extract is 35-40Mpa, and extraction temperature is 45-55 DEG C, extraction
Time is 160-210min, CO2The flow of fluid is 15-22kg/h.
Preferably, the quality of effective component of chinese medicine described in S3 and microbial bacterial agent is 1:0.3-0.5；The microorganism
Microbial inoculum is EM bacteria agent.
Preferably, maintaining temperature to maintain dimension at 35-38 DEG C at 48-50 DEG C, anaerobic fermentation when aerobic fermentation in S3；
Maintain temperature at 26-28 DEG C when fermenting in S4.
The present invention also provides the application methods of the prevention and treatment ginger root knot nematode disease plant medical agent, comprising the following steps:
(1) ginger kind is handled: ginger kind being soaked in 250-350 times of liquid of plant medical agent and is sterilized 10-12min；
(2) kind planting ditch processing: with 400-600 times of liquid of plant medical agent sprinkling kind planting ditch to wet.
Compared with prior art, the invention has the following beneficial effects:
The present invention screens plurality of Chinese raw material, passes through CO2Supercritical extract effectively extracts wherein effective component, then cooperates
Microbial bacterial agent carries out secondary fermentation, is prepared into novel plant medicament, is rich in a variety of insecticidal constituents, phase between each effective component
Mutually synergistic effect, effectively prevents and kill off root-knot nematode by the different mechanism of action, significantly improves vermins-proof effect；It is also raw rich in plant
Long required nutrient, can promote plant robust growth, improve crop disease-resistant ability；In addition plant medical agent pair of the invention
Non-target organism safety, pesticide residue is low, can mitigate the pressure of environment, is a kind of green medicament of environment-friendly type.
Specific embodiment
Specific embodiments of the present invention will be described in detail below, it is to be understood that protection scope of the present invention is not
It is restricted by specific implementation.
Embodiment 1
A kind of preparation method of plant medical agent that preventing and treating root knot nematode disease, comprising the following steps:
S1. weigh by weight ratio: tea seed cake 16g, ginger 21g, garlic 20g, rotenone 25g, banyan root 16g, tree-of-heaven 11g,
Black false hellebore 16g, matrine 24g, Tobacco Leaf 16g, radix gentianae 25g, Chinese prickly ash 16g, margosa 24g, betel nut 20g and native ash 7g；And it will
Each raw material component drying to moisture content is 5wt%, crushed 45 meshes, obtains traditional Chinese medicine powder；
S2. extractant petroleum ether will be added in traditional Chinese medicine powder, is added in extraction kettle carries out CO after mixing2Overcritical extraction
It takes, obtains extract；Extract is centrifugated and removes impurity, recycling extractant obtains effective component of chinese medicine；
S3. will in effective component of chinese medicine be added EM microbial bacterial agent, after mixing first carry out 10d aerobic fermentation, then into
The anaerobic fermentation of row 25d filters after fermentation, obtains primary fermentation object；
S4. primary fermentation object is placed in a greenhouse and continues the 45d that ferments, filtered after fermentation, obtain plant medical agent；
Wherein, the mass ratio of traditional Chinese medicine powder described in S2 and petroleum ether is 1:0.04；The CO2The pressure of supercritical extract is
35Mpa, extraction temperature are 45 DEG C, extraction time 160min, CO2The flow of fluid is 15kg/h；Chinese medicine described in S3 is effective
The quality of ingredient and EM bacteria agent is 1:0.3；Temperature is maintained to maintain dimension 35 at 48 DEG C, anaerobic fermentation when aerobic fermentation in S3
℃；Maintain temperature at 26 DEG C when fermenting in S4.
Embodiment 2
A kind of preparation method of plant medical agent that preventing and treating root knot nematode disease, comprising the following steps:
S1. it weighs by weight ratio: tea seed cake 12g, ginger 14g, garlic 14g, rotenone 20g, banyan root 13g, tree-of-heaven 9g, Chenopodiaceae
Reed 11g, matrine 20g, Tobacco Leaf 13g, radix gentianae 20g, Chinese prickly ash 13g, margosa 22g, betel nut 15g and native ash 6g；And it will be each
Raw material components drying to moisture content is 6wt%, crushed 52 meshes, obtains traditional Chinese medicine powder；
S2. extractant petroleum ether will be added in traditional Chinese medicine powder, is added in extraction kettle carries out CO after mixing2Overcritical extraction
It takes, obtains extract；Extract is centrifugated and removes impurity, recycling extractant obtains effective component of chinese medicine；
S3. will in effective component of chinese medicine be added EM microbial bacterial agent, after mixing first carry out 13d aerobic fermentation, then into
The anaerobic fermentation of row 27d filters after fermentation, obtains primary fermentation object；
S4. primary fermentation object is placed in a greenhouse and continues the 52d that ferments, filtered after fermentation, obtain plant medical agent；
Wherein, the mass ratio of traditional Chinese medicine powder described in S2 and petroleum ether is 1:0.06；The CO2The pressure of supercritical extract is
37Mpa, extraction temperature are 50 DEG C, extraction time 185min, CO2The flow of fluid is 18kg/h；Chinese medicine described in S3 is effective
The quality of ingredient and EM bacteria agent is 1:0.4；Temperature is maintained to maintain dimension 37 at 49 DEG C, anaerobic fermentation when aerobic fermentation in S3
℃；Maintain temperature at 27 DEG C when fermenting in S4.
Embodiment 3
A kind of preparation method of plant medical agent that preventing and treating root knot nematode disease, comprising the following steps:
S1. weigh by weight ratio: tea seed cake 16g, ginger 21g, garlic 20g, rotenone 25g, banyan root 16g, tree-of-heaven 11g,
Black false hellebore 16g, matrine 24g, Tobacco Leaf 16g, radix gentianae 25g, Chinese prickly ash 16g, margosa 24g, betel nut 20g and native ash 7g；And it will
Each raw material component drying to moisture content is 7wt%, crushed 60 meshes, obtains traditional Chinese medicine powder；
S2. extractant petroleum ether will be added in traditional Chinese medicine powder, is added in extraction kettle carries out CO after mixing2Overcritical extraction
It takes, obtains extract；Extract is centrifugated and removes impurity, recycling extractant obtains effective component of chinese medicine；
S3. will in effective component of chinese medicine be added EM microbial bacterial agent, after mixing first carry out 16d aerobic fermentation, then into
The anaerobic fermentation of row 30d filters after fermentation, obtains primary fermentation object；
S4. primary fermentation object is placed in a greenhouse and continues the 60d that ferments, filtered after fermentation, obtain plant medical agent；
Wherein, the mass ratio of traditional Chinese medicine powder described in S2 and petroleum ether is 1:0.08；The CO2The pressure of supercritical extract is
40Mpa, extraction temperature are 55 DEG C, extraction time 210min, CO2The flow of fluid is 22kg/h；Chinese medicine described in S3 is effective
The quality of ingredient and EM bacteria agent is 1:0.5；Temperature is maintained to maintain dimension 38 at 50 DEG C, anaerobic fermentation when aerobic fermentation in S3
℃；Maintain temperature at 28 DEG C when fermenting in S4.
Comparative example 1
The raw material components of plant medical agent are as follows: ginger 21g, garlic 20g, rotenone 25g, banyan root 16g, tree-of-heaven 11g, black false hellebore
16g, matrine 24g, radix gentianae 25g, Chinese prickly ash 16g, margosa 24g and native ash 7g；Other operations are carried out according to embodiment 1.
Comparative example 2
The raw material components of plant medical agent are as follows: tea seed cake 16g, garlic 20g, rotenone 25g, banyan root 16g, black false hellebore 16g, kuh-seng
Alkali 24g, Tobacco Leaf 16g, radix gentianae 25g, margosa 24g, betel nut 20g and native ash 7g；Other operations are carried out according to embodiment 1.
Comparative example 3
The raw material components of plant medical agent are as follows: tea seed cake 16g, ginger 21g, rotenone 25g, banyan root 16g, tree-of-heaven 11g, matrine
24g, Tobacco Leaf 16g, radix gentianae 25g, Chinese prickly ash 16g, betel nut 20g and native ash 7g；Other operations are carried out according to embodiment 1.
Comparative example 4
The raw material components of plant medical agent are as follows: tea seed cake 16g, ginger 21g, rotenone 25g, banyan root 16g, tree-of-heaven 11g, black false hellebore
16g, matrine 24g, Tobacco Leaf 16g, Chinese prickly ash 16g, margosa 24g and native ash 7g；Other operations are carried out according to embodiment 1.
Comparative example 5
The raw material components of plant medical agent are as follows: tea seed cake 16g, ginger 21g, garlic 20g, rotenone 25g, tree-of-heaven 11g, black false hellebore 16g,
Matrine 24g, Tobacco Leaf 16g, radix gentianae 25g, Chinese prickly ash 16g, margosa 24g and betel nut 20g；Other operations are according to embodiment 1
It carries out.
Field control effectiveness test
Experimental design: test research group 1, study group 2, study group 3, control group 1, control group 2, control group 3, control
Organize 4, control group 5, blank control group totally 9 test groups；Wherein study group 1 to study group 3 sprays embodiment 1 to embodiment respectively
The plant medical agent of 3 preparations；Control group 1 to control group 5 sprays the plant medical agent that comparative example 1 is prepared to comparative example 5 respectively；Blank pair
Sterile water is sprayed according to group.
Test operation: cell, each cell 2m are set in ginger cultivation, management condition the same plot2, 1d before the application
And application 7d, 12d geotome acquires the soil sample of five points around race, depth selection 15cm, every takes 20g soil sample,
Acquisition separates root-knot nematode using funnel method, counts root-knot nematode quantity by magnifying glass, calculates nematode decline rate and prevention and treatment effect
Fruit, test result see the table below 1.
Nematode decline rate (%)=[hot line borer population before (hot line borer population after hot line borer population-application before being administered) ÷ is administered] ×
100；
Control efficiency (%)=[(treatment region nematode decline rate-blank district nematode decline rate) ÷ (100-blank pair
According to area's nematode decline rate)] × 100；
The control efficiency of the plant medical agent of 1 present invention prevention and treatment root knot nematode disease of table
As it can be seen from table 1 the nematode decline rate and control efficiency of 1-3 of the embodiment of the present invention are obviously higher than each control
Group illustrates that plant medical agent of the invention can effectively prevent ginger root-knot nematode, to improve the yield and quality of ginger.
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and its actually answering
With so that those skilled in the art can be realized and utilize a variety of different exemplary implementation schemes of the invention and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (8)
1. a kind of plant medical agent for preventing and treating ginger root knot nematode disease, which is characterized in that the raw material of the plant medical agent is by weight
Count composed of the following components: 8-16 parts of tea seed cake, 7-21 parts of ginger, 8-20 parts of garlic, 15-25 parts of rotenone, 10-16 parts of banyan root,
7-11 parts of tree-of-heaven, 6-16 parts of black false hellebore, 16-24 parts of matrine, 10-16 parts of Tobacco Leaf, 15-25 parts of radix gentianae, 10-16 parts of Chinese prickly ash,
20-24 parts of margosa, 10-20 parts of betel nut and native 5-7 parts of ash.
2. the plant medical agent of prevention and treatment ginger root knot nematode disease according to claim 1, which is characterized in that the plant medical agent
Raw material it is composed of the following components by weight: 12 parts of tea seed cake, 14 parts of ginger, 14 parts of garlic, 20 parts of rotenone, banyan root 13
Part, 9 parts of tree-of-heaven, 11 parts of black false hellebore, 20 parts of matrine, 13 parts of Tobacco Leaf, 20 parts of radix gentianae, 13 parts of Chinese prickly ash, 22 parts of margosa, betel nut
15 parts and 6 parts of soil ash.
3. the preparation method of prevention and treatment ginger root knot nematode disease plant medical agent according to claim 1 or 2, which is characterized in that
The following steps are included:
S1. meter weighs each raw material component by weight ratio, is 5-7wt% by each raw material component drying to moisture content, crushes
45-60 mesh is crossed, traditional Chinese medicine powder is obtained；
S2. extractant will be added in traditional Chinese medicine powder, be added in extraction kettle carry out CO after mixing2Supercritical extract is extracted
Object；Extract is centrifugated and removes impurity, recycling extractant obtains effective component of chinese medicine；
S3. microbial bacterial agent will be added in effective component of chinese medicine, first carry out 10-16d aerobic fermentation after mixing, then carry out
The anaerobic fermentation of 25-30d filters after fermentation, obtains primary fermentation object；
S4. primary fermentation object is placed in a greenhouse and continues the 45-60d that ferments, filtered after fermentation, obtain plant medical agent.
4. preparation method according to claim 3, which is characterized in that the mass ratio of traditional Chinese medicine powder described in S2 and extractant is
1:0.04-0.08；The extractant is petroleum ether.
5. preparation method according to claim 3, which is characterized in that CO described in S22The pressure of supercritical extract is 35-
40Mpa, extraction temperature are 45-55 DEG C, extraction time 160-210min, CO2The flow of fluid is 15-22kg/h.
6. preparation method according to claim 3, which is characterized in that effective component of chinese medicine described in S3 and microbial bacterial agent
Quality be 1:0.3-0.5；The microbial bacterial agent is EM bacteria agent.
7. preparation method according to claim 3, which is characterized in that maintain temperature at 48-50 DEG C when aerobic fermentation in S3,
Maintain dimension at 35-38 DEG C when anaerobic fermentation；Maintain temperature at 26-28 DEG C when fermenting in S4.
8. according to claim 1 or 2 prevention and treatment ginger root knot nematode disease plant medical agents application method, which is characterized in that including with
Lower step:
(1) ginger kind is handled: ginger kind being soaked in 250-350 times of liquid of plant medical agent and is sterilized 10-12min；
(2) kind planting ditch processing: with 400-600 times of liquid of plant medical agent sprinkling kind planting ditch to wet.
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| CN115428789A (en) * | 2022-08-25 | 2022-12-06 | 雨木林风(云南)科技有限公司 | Snail prevention and treatment agent and preparation and use methods thereof |
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Application publication date: 20191022 |