CN105706806A - Method for preventing and controlling daktulosphaira vitifolia fitch by utilizing plant source preparation - Google Patents
Method for preventing and controlling daktulosphaira vitifolia fitch by utilizing plant source preparation Download PDFInfo
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- CN105706806A CN105706806A CN201610167825.1A CN201610167825A CN105706806A CN 105706806 A CN105706806 A CN 105706806A CN 201610167825 A CN201610167825 A CN 201610167825A CN 105706806 A CN105706806 A CN 105706806A
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- daktulosphaira
- vitifolia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Toxicology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for preventing and controlling daktulosphaira vitifolia fitch by utilizing a plant source preparation. The method comprises the following steps of using tobacco, blumea balsamifera, radix astragali, radix tripterygii wilfordii, radix sophorae flavescentis, fructus toosendan and herba andrographitis as plant raw materials, and preparing the plant raw materials into plant extract liquor after extracting; independently or compositely using the plant extract liquor according to the soil texture and the damage degree of the daktulosphaira vitifolia fitch, irrigating diluted plant extract liquor to soil at the root of a grape plant after the plant extract liquor is diluted by water according to a volume ratio of 20 to 50 times, and irrigating for 4 to 6 times every year, wherein the plant extract liquor can also be applied with irrigation water. According to the method for preventing and controlling the daktulosphaira vitifolia fitch by utilizing the plant source preparation, disclosed by the invention, the plant water extract liquor having pest inhibiting and pest killing functions is firstly used for evaluating a prevention and control effect of the daktulosphaira vitifolia fitch; compared with an existing chemical insecticide available in the market, the method has the advantages that environmental friendliness is realized, grape tree bodies are not damaged, grape fruits are not polluted, application is convenient and safe, daktulosphaira vitifolia fitch groups can be sustainably and effectively controlled, and the method is suitable for being used in a fruit garden which suffers from the daktulosphaira vitifolia fitch already and still keeps commercial production.
Description
One, technical field
The present invention relates to a kind of method utilizing plant source preparation prevention and control grape phylloxera, be specifically related to one and utilize cigarette
The Chinese distinctive Chinese herbal medicine lixiviating solution such as grass, Herba Blumeae Balsamiferae, the Radix Astragali, Radix Tripterygii Wilfordii, Radix Sophorae Flavescentis, Fructus Toosendan are to Fructus Vitis viniferae obligatory parasitism evil
Worm grape phylloxera carries out the method prevented and treated, and belongs to lobesia botrana field of biological control.
Two, background technology
Grape phylloxera (Daktulosphaira vitifolia Fitch) is a kind of colonize on grape root system, body
The least quarantine pest insect.Grape phylloxera, by taking food grape root system to directly affecting the conveying of Fructus Vitis viniferae nutrition and moisture, is made
Becoming tree vigo(u)r weak, its root knot formed and root nodule cause root system to rot after festering, and cause vine asthenia to be died.18 end of the centurys,
Grape phylloxera travels to Europe from America, result in the vitis vinifera entire PLA destruction of sensitivity, and Producer has to use
Some the Fructus Vitis viniferae kinds coming from America carry out grafting cultivation, therefore current Fructus Vitis viniferae main product state the most in the world as Resistant rootstock, except
China, the most all uses the cultivation mode of Resistant rootstock grafting.China does not has spreading of radicola to cause harm due to long-term closing,
Having been used up cultivating from root of cultivar, the convenience introduced in recent years along with reform and opening-up and nursery stock, nursery stock circulates freely,
Cause the propagation increased risk of radicola.Found since radicola in Prospirolboli joannsi vineyard, Shanghai from 2005, the most in
The multiple provinces and regions of state, as the ground such as Hunan, Guizhou, Shaanxi, Liaoning find that radicola is caused harm, wherein, nearly 100,000 mu of area, Hunan Huaihua
Chinese Vitis davidi be injured the most serious.Orchard worker's Fructus Vitis viniferae of being both unwilling for economic interests directly will to be injured is excluded, and wishes again to tie up
Hold the production capacity of Fructus Vitis viniferae, therefore, prevent and treat the most safely and the most online radicola and become one of problem needing solution,
The present invention answers this demand to conduct a research.
Abroad the vineyard that grape phylloxera occurs mainly is used the method pulling out Fructus Vitis viniferae, coordinate and use some to have smoked
The chemical insecticide of steaming effect, as DD mixture carries out fumigation to soil, but not yet has still growing solid vine
Carrying out the successful example of chemical insecticide preventing and treating, main cause is that the character of soil causes general insecticide effect poor, and field
Between a large amount of use efficient pesticides not only to cause harm Growth status, cause fruit pesticide residues, and have and cause environment water to pollute
Risk.Biological control is an important directions of Pest control in the last few years, the main collection of Biological control research of grape phylloxera
In utilizing insect pathogenic fungus to control in terms of radicola.Pesticide plant and Fructus Vitis viniferae is currently utilized to carry out catch cropping to control root nodule
The report of aphid, Guo Qing etc. (2011) chooses Schizonepeta tenuifolia, Radix Scutellariae and three kinds of plant speciess of the Radix Astragali and is implanted in the row that grape phylloxera occurs
Between, result shows that the recovery of prevention and control grape phylloxera and tree vigo(u)r is had certain effect.Infection root nodule is thought in Wang (2014) research
Catch cropping Nicotiana tabacum L. in the vineyard of aphid, can produce certain inhibitory action to the radicola of Fructus Vitis viniferae root.Catch cropping is thought in this research
Thing root system is typically limited with the root system of Fructus Vitis viniferae handing-over position, and therefore prevention effect is the most limited.So far, the most also there is no profit
Report with the document of plant source preparation prevention and control grape phylloxera.
Three, summary of the invention
Solved by the invention haveing a problem in that provides a kind of green, effectively control grape phylloxera to infect the side of Fructus Vitis viniferae
Method.Content includes the preparation method of various vegetable source pesticide material and for the application process of Fructus Vitis viniferae of being injured.
The technical problem to be solved in the present invention is by the following technical solutions.
A kind of method utilizing plant source preparation prevention and control grape phylloxera, is by Nicotiana tabacum L., Herba Blumeae Balsamiferae, the Radix Astragali, Radix Tripterygii Wilfordii, hardship
Ginseng, Fructus Toosendan and Herba Andrographis are as plant material, respectively according to the solid-liquid ratio (mass ratio) of 1:1 after being pulverized by every kind of plant material
Add water and be configured to the stock solution of every kind of raw material, various stock solutions are respectively placed in boiling water bath extraction 30 minutes, take after filtered while hot
Supernatant;Every kind of supernatant is settled to extract front stock solution volume with water respectively and i.e. obtains the plant lixiviating solution of every kind of raw material;Will
Seven kinds of plant lixiviating solution are stored in containers for future use respectively;During use, according to the soil texture and the radicola extent of injury, individually or
The above-mentioned plant lixiviating solution of compounding use, waters Fructus Vitis viniferae and plants after being diluted according to its volume with water of 20-50 times by plant lixiviating solution
Strain root soil, annual 4-6 time, also can use with irrigation water.During compounding use, with the one in above-mentioned seven kinds of plant lixiviating solution
Plant lixiviating solution is main component, and the cumulative volume of other one or more plant lixiviating solution is less than this extraction liquid that grows tobacco
Long-pending.
The method have the benefit that
The present invention utilizes first to have and presses down the vegetation water lixiviating solution of worm and insecticidal action grape phylloxera is carried out prevention and control effect
Fruit is evaluated.Compared with chemical insecticide existing with on market, the present invention has environmental friendliness, to vine body fanout free region, to Portugal
Grape fruit is pollution-free, uses convenient and safe, sustainable effective control radicola colony, is suitable for having occurred and that radicola but still protects
Hold orchard worker's application of commercial production.
Four, accompanying drawing explanation
Fig. 1 Nicotiana tabacum L. and Radix Astragali lixiviating solution are individually and complex treatment radicola worm's ovum and the 24h mortality rate of nymph;
In Fig. 1, the Radix Astragali is Radix Astragali lixiviating solution;Nicotiana tabacum L. is Nicotiana tabacum L. lixiviating solution;Mixture is Radix Astragali lixiviating solution and Nicotiana tabacum L. lixiviating solution
The Nicotiana tabacum L. Radix Astragali mixing lixiviating solution made is compounded according to the volume ratio of 1:1;Comparison is water;Fig. 1 shows, in the present invention, Nicotiana tabacum L. is with yellow
Stilbene lixiviating solution uses according to the ratio of 1:1 is compounding, and the control action ratio of grape phylloxera worm's ovum is administered alone Nicotiana tabacum L. lixiviating solution
Or Radix Astragali lixiviating solution is more preferable.
Detailed description of the invention
Embodiment 1
1, acquired for materials and process
By the vegetable material of acquired Radix Tripterygii Wilfordii, Nicotiana tabacum L., the Radix Astragali, Fructus Toosendan, Semen Hyoscyami, Herba Blumeae Balsamiferae and Herba Andrographis 50
Pulverize after drying under conditions of DEG C, take the boiling water extraction in the distilled water of 500mL of the vegetable material after 500g pulverizes respectively
30min, takes supernatant after double gauze filters, adds water and be settled to 500mL, i.e. obtain plant lixiviating solution, soaked by various plants
Extract preserves under the conditions of being individually placed in 4 DEG C.In order to verify the plant lixiviating solution control action to radicola nymph in the present invention, real
(spiral shell worm ethyl ester effective ingredient is 240g L to test middle selection mu Wang Te-1, Bayer Bitterfeld GmbH crop science company) and as comparison parasite killing
Agent, uses Bayer recommended density 800ug mL-1。
Bottom the culture dish of a diameter of 9cm, put the filter paper of a diameter of 9cm, filter paper instills 1.5mL system of the present invention
Standby plant lixiviating solution, puts into and the filter paper of first culture dish formed objects bottom the culture dish of another same diameter,
And instill the aquesterilisa of equivalent as comparison.Each culture dish is placed identical age in days 2 age nymph 30, each process 5
Repeat.After being disposed, useSeal each culture dish, prevent radicola escape, finally each culture dish is placed in
24 DEG C, relative humidity be 60% constant incubator in light culture.The 24th, 48,72 and 96h records the most after treatment are dead
Die number (touch examination worm polypide and foot with brush pen, the most motionless be considered as death), calculate the corrected mortality of radicola nymph,
Test result indicate that (seeing attached list 1), each plant lixiviating solution related in the present invention, processing grape phylloxera respectively
After nymph, the survival rate of radicola nymph all has decline in various degree, and along with the prolongation of the time of process, the school of radicola
Positive mortality rate increases.This explanation plant lixiviating solution prepared by the present invention is in the case of using respectively, if to grape phylloxera
Worm is respectively provided with and significantly controls effect, and has regular hour accumulative effect.
The corrected mortality of grape phylloxera nymph after the process of subordinate list 1 plant lixiviating solution
Note: different lower cases represent the significant difference (P < 0.05) between same index different disposal group, lower same.
Embodiment 2
1, acquired for materials and process:
Host material is annual Cabernet Sauvignon cutting seedling, (is infected formation root knot, test Radix vitis viniferae by grape phylloxera
Tumor aphid is Shaanxi Ba Qiao biotype), test and carried out in Shandong Agricultural University greenhouse in 2013.Use respectively and used water dilute
Release the tobacco extract after 20 times (cigarette process) and Fructus Vitis viniferae that the pouring of every 2 days of tap water (CK) is infected be potted plant, pouring one week altogether,
After process terminates, test seedling is removed, observe root situation.After Nicotiana tabacum L. lixiviating solution processes, Cabernet Sauvignon cutting seedling root is formed
Root knot number is decreased obviously, and waters the matched group of tap water, and root knot number does not has significant change.Meanwhile, pouring Nicotiana tabacum L. lixiviating solution
After Cabernet Sauvignon cutting seedling improving activity of root system and matched group without significant difference.This explanation, the tobacco plant selected in present invention extraction
Grape phylloxera is infected the root knot of formation and has significant inhibitory action by liquid, and grape root system vigor is not had bad shadow
Ring.
Embodiment 3
1, acquired for materials and process:
According to the operational approach in embodiment 1, if four process, use respectively the present invention mentions Radix Astragali lixiviating solution,
(Radix Astragali lixiviating solution and Nicotiana tabacum L. lixiviating solution are mixed according to the volume ratio of 1:1 for Nicotiana tabacum L. lixiviating solution and Nicotiana tabacum L. Radix Astragali mixing lixiviating solution
Compounding Nicotiana tabacum L. Radix Astragali mixing lixiviating solution);To use water for comparison;The worm's ovum of grape phylloxera and the dead of nymph is calculated after 24h
Die rate.Test result indicate that, in the present invention, Nicotiana tabacum L. uses, to grape phylloxera according to the ratio of 1:1 is compounding with Radix Astragali lixiviating solution
The control action ratio of worm's ovum is administered alone Nicotiana tabacum L. lixiviating solution or Radix Astragali lixiviating solution is more preferable (Fig. 1).
Claims (2)
1. the method utilizing plant source preparation prevention and control grape phylloxera, it is characterised in that be by Nicotiana tabacum L., Herba Blumeae Balsamiferae, the Radix Astragali,
Radix Tripterygii Wilfordii, Radix Sophorae Flavescentis, Fructus Toosendan and Herba Andrographis are as plant material, respectively according to the quality of 1:1 after being pulverized by every kind of plant material
It is configured to the stock solution of every kind of raw material than addition water, various stock solutions is respectively placed in boiling water bath extraction 30 minutes, after filtered while hot
Take supernatant;Before every kind of supernatant is settled to extraction with water respectively, its stock solution volume i.e. obtains the plant extraction of every kind of raw material
Liquid;Seven kinds of plant lixiviating solution are stored in containers for future use respectively;During use, according to the soil texture and the radicola extent of injury,
Individually or compounding use above-mentioned plant lixiviating solution, pouring after plant lixiviating solution is diluted according to its volume with water of 20-50 times
Grapevine seedling root soil, annual 4-6 time, also can use with irrigation water.
A kind of method utilizing plant source preparation prevention and control grape phylloxera, it is characterised in that compounding
During use, with certain lixiviating solution that grows tobacco as main component, the cumulative volume of other one or more plant lixiviating solution is less than this certain
Grow tobacco lixiviating solution volume.
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106212158A (en) * | 2016-07-27 | 2016-12-14 | 普定县顺和水果苗木种植有限公司 | A kind of planting technology of dinosaurian eggs Lee |
CN107114203A (en) * | 2017-07-03 | 2017-09-01 | 合肥卓畅农业科技有限公司 | A kind of grape planting process |
CN107156193A (en) * | 2017-06-07 | 2017-09-15 | 江苏农牧科技职业学院 | The medicine based on herbs and its preparation application method of apocarya police radicola |
CN107593794A (en) * | 2017-10-31 | 2018-01-19 | 桐梓县茅石乡龙会村蔬菜种植场 | A kind of insecticide for wine-growing and preparation method thereof |
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-
2016
- 2016-03-23 CN CN201610167825.1A patent/CN105706806A/en active Pending
Cited By (8)
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CN106212158A (en) * | 2016-07-27 | 2016-12-14 | 普定县顺和水果苗木种植有限公司 | A kind of planting technology of dinosaurian eggs Lee |
CN107156193A (en) * | 2017-06-07 | 2017-09-15 | 江苏农牧科技职业学院 | The medicine based on herbs and its preparation application method of apocarya police radicola |
CN107156193B (en) * | 2017-06-07 | 2020-08-25 | 江苏农牧科技职业学院 | Botanical pesticide of carya illinoensis and armillaria rhynchophylla, and preparation and use methods thereof |
CN107114203A (en) * | 2017-07-03 | 2017-09-01 | 合肥卓畅农业科技有限公司 | A kind of grape planting process |
CN107593794A (en) * | 2017-10-31 | 2018-01-19 | 桐梓县茅石乡龙会村蔬菜种植场 | A kind of insecticide for wine-growing and preparation method thereof |
CN107788034A (en) * | 2017-11-01 | 2018-03-13 | 昭平县科学技术实验场 | A kind of plant pesticide effervescent tablet |
CN107788035A (en) * | 2017-11-01 | 2018-03-13 | 昭平县科学技术实验场 | A kind of plant pesticide |
CN109769872A (en) * | 2017-11-13 | 2019-05-21 | 江苏小潘园生态农业科技发展有限公司 | A kind of wine-growing insecticide |
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