KR100513258B1 - Controller of plant disease and noxious insects using herb medicine extracts and producing method thereof - Google Patents

Controller of plant disease and noxious insects using herb medicine extracts and producing method thereof Download PDF

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KR100513258B1
KR100513258B1 KR10-2002-0046124A KR20020046124A KR100513258B1 KR 100513258 B1 KR100513258 B1 KR 100513258B1 KR 20020046124 A KR20020046124 A KR 20020046124A KR 100513258 B1 KR100513258 B1 KR 100513258B1
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herbal
present
pest control
treatment
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KR10-2002-0046124A
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Korean (ko)
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KR20020068021A (en
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박지웅
정수권
이인수
강수웅
김정수
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케이지바텍 주식회사
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/10Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/12Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/34Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/46Stemonaceae [Stemona family], e.g. croomia

Abstract

본 발명은 한약재를 혼합, 추출, 여과하는 단계를 포함하는 한약추출물을 이용한 식물병·해충 방제제의 제조에 있어서, 한약재는 천문동, 빈랑자, 백부근, 천련자, 정향, 건강, 지유, 사군자, 지모, 대계, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑죽, 포공영, 연교, 금은화, 여로, 건칠피, 용뇌, 연초엽으로 하고, 혼합단계에서 한약재의 농도는 각각 0.003~0.024 중량%인 것을 특징으로 하고, 여과단계에서 맥반석 가루는 0.001~0.01 중량%인 것을 특징으로 하는 한약추출물을 이용한 식물병·해충 방제제의 제조방법에 관한 것으로, 본 발명에 의해 제조된 한약추출물을 이용한 식물병·해충 방제제는 식물의 병과 해충에 대한 방제효과가 뛰어날 뿐만 아니라 식물에 약해를 주지 않고 오히려 생장을 촉진시키는 효과가 있으므로 화학농약의 대체 제제로 유용하게 사용될 수 있는 탁월한 효과가 있다.The present invention in the manufacture of plant diseases and pest control agents using herbal extracts comprising the steps of mixing, extracting, and filtering herbal medicines, the herbal medicines are astronomical dong, betel nut, baekbugeun, cheonngija, cloves, health, fat milk, Sagunja, Jimo, Daegyeul, Hakseol, Hwangbaekpi, casualties, kegro, Gilgyeong, Hagocho, love leaf, gallja, black porridge, pogongyoung, yeonkyo, gold silver flower, Yeoro, dried Chilpipi, dragon brain, tobacco leaf, the concentration of herbs in the mixing phase is 0.003 It is characterized by a ~ 0.024% by weight, the ganban stone powder in the filtration step relates to a method for producing a plant disease, pest control agent using a herbal extract, characterized in that 0.001 ~ 0.01% by weight, the Chinese medicine prepared by the present invention Plant diseases and pest control agents using extracts are not only excellent in controlling the diseases and pests of plants but also do not harm the plants, rather they promote the growth of chemical pesticides. It has an excellent effect that can be usefully zero.

Description

한약추출물을 이용한 식물병해충 방제제 및 그 제조방법 {Controller of plant disease and noxious insects using herb medicine extracts and producing method thereof}Controller of plant disease and noxious insects using herb medicine extracts and producing method

본 발명은 한약재를 혼합, 추출, 여과하는 단계를 포함하는 한약추출물을 이용한 식물병·해충 방제제의 제조방법과 그 방법으로 제조된 한약추출물을 이용한 식물병·해충 방제제에 관한 것이다. The present invention relates to a method for producing plant diseases and pest control agents using herbal extracts comprising the steps of mixing, extracting and filtering herbal medicines and plant diseases and pest control agents using herbal extracts prepared by the method.

현재 우리는 식량 걱정을 하지 않아도 되는 시대에 살고 있지만, 농산물을 증대하는데 있어 여러 가지 증산을 위한 기술 외에도 병·해충의 효과적인 방제를 통해 농산물을 증대하는 것이 가장 중요하다. 60~70년대까지만 하더라도 식량증산을 위한 대표적인 방법으로 대부분 유기합성 농약을 사용하였다. 이러한 화학농약의 일변도는 점차적으로 많은 부작용을 초래하게 되었는데, 그 대표적인 예로 환경오염, 병해충 내성 및 저항성 그리고 인축에 대한 농약 잔류성 등이 사회적 문제가 되었다. 그래서 80~90년도부터 목초액, 각종 미생물 제제, 병·해충에 저항성이 강한 품종의 육종 등 여러 부분에서 농약사용 저감을 위한 새로운 대체 방법 개발이 활발히 진행되고 있지만, 아직은 미흡한 문제점이 있다. We live in an age where we don't have to worry about food, but it is most important to increase agricultural products through effective control of diseases and pests in addition to various production techniques. Until the 1960s and 1970s, most organic synthetic pesticides were used as a representative method for food production. The univariate of such chemical pesticides has caused many side effects gradually. For example, environmental pollution, pest resistance and resistance, and pesticide residues on human beings have become social problems. Therefore, since 80-90, new alternative methods for reducing pesticide use have been actively developed in various parts such as wood vinegar, various microbial preparations, breeding of varieties resistant to diseases and pests, but there are still insufficient problems.

본 발명 한약추출물을 이용한 식물병·해충 방제제를 발명하게 된 근본 취지는 유기합성농약의 대체, 농산물의 증수, 병·해충방제 및 생장촉진효과를 동시에 나타냄으로써 인력 및 생산비용 절감과 그로 인한 국내·외 농산물의 경쟁력 향상에 있다. 그리고 농작물을 재배하는 농민과 이를 이용하는 소비자의 국민건강 증진 기여에 있다. The basic purpose of the invention of plant diseases and pest control agents using the herbal extracts of the present invention is to replace the organic synthetic pesticides, increase the production of agricultural products, disease and pest control and growth promoting effect simultaneously to reduce the manpower and production costs and thereby domestic · Increase competitiveness of agricultural products. And it contributes to the promotion of national health of farmers who grow crops and consumers who use them.

따라서, 본 발명의 목적은 한약재를 혼합, 추출, 여과하는 단계를 포함하는 하여 한약추출물을 이용한 식물병·해충 방제제의 제조방법을 제공함에 있다. 본 발명의 다른 목적은 상기 제조방법으로 제조된 한약추출물을 이용한 식물병·해충 방제제를 제공함에 있다. Accordingly, an object of the present invention is to provide a method for producing a plant disease, pest control agent using the herbal extracts, including the step of mixing, extracting, and filtering the herbal medicines. Another object of the present invention to provide a plant disease, pest control agent using the herbal extract prepared by the above method.

본 발명의 상기 목적은 한약재를 혼합, 추출, 여과하여 한약추출물을 제조하고, 상기 제조된 한약추출물의 식물에 대한 살균, 살충, 약해여부 및 생장촉진효과를 확인함으로써 달성하였다.The object of the present invention was achieved by mixing, extracting, and filtering herbal medicines to produce herbal extracts, and confirming the bactericidal, insecticidal, weakening and growth promoting effects on the plants of the prepared herbal extracts.

본 발명 한약추출물을 이용한 식물병·해충 방제제는 한약재를 물과 혼합하는 단계; 상기 혼합물을 약탕기에서 10시간 이상 달여서 추출하는 단계; 상기 추출물에 맥반석 가루를 넣고 교반한 후 침전시켜 여과하는 단계를 포함하여 제조된다.Plant disease, pest control agent using the herbal extracts of the present invention is a step of mixing the herbal medicine with water; Extracting the mixture by decoction over 10 hours in a shaker; It is prepared by putting the ganban stone powder in the extract and then stirring the precipitate.

상기 한약재는 바람직하게는 천문동, 빈랑자, 백부근, 천련자, 정향, 건강, 지유, 사군자, 지모, 대계, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑죽, 포공영, 연교, 금은화, 여로, 건칠피, 용뇌, 연초엽으로 한다. The herb medicine is preferably astronomical dong, betel nut, baekbugeun, cheonngija, cloves, health, fat, four seasons, gimo, Daegye, Hakseul, Hwangbaekpi, casualties, kegyeong, Gilgyeong, Hagocho, love leaf, fivefold, black porridge, pogongyoung, yeonkyo , Gold and silver coins, erosion, dry skin, brain, soft leaf.

상기 혼합단계에서 한약재의 농도는 바람직하게는 각각 0.003~0.024 중량%으로 한다. 더욱 바람직하게는 천문동, 빈랑자, 백부근, 천련자는 0.0086 중량%, 정향, 건강, 지유, 사군자, 지모, 대계는 0.0057 중량%, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑죽, 포공영, 연교, 금은화, 여로는 0.0029 중량%, 건칠피, 용뇌는 0.0086 중량%, 연초엽은 0.0171 중량%로 한다. The concentration of the herbal medicine in the mixing step is preferably 0.003 to 0.024% by weight, respectively. More preferably, the astronomical dong, betel nut, baekbugeun, cheonnyeonul, 0.0086% by weight, cloves, health, fat, four seasons, gimo, Daegye, 0.0057% by weight, Hakseul, Hwangbaekpi, casualties, neck, Gilgyeong, Hagocho, love leaf, gall bladder, Black porridge, pogongyoung, yeonkyo, gold and silver flower, 0.0029% by weight, kilchipi, kyongil 0.0086% by weight, edible leaf 0.0171% by weight.

상기 여과단계에서 맥반석 가루의 농도는 바람직하게는 0.001~0.01 중량%으로 한다.In the filtration step, the concentration of elvan powder is preferably 0.001 to 0.01 wt%.

상기 각각의 한약재의 농도는 0.003중량% 이하에서는 그 효과가 거의 나타나지 않으며, 0.024중량% 이상에서는 식물의 생장이 오히려 저하된다. The concentration of each herbal is less than 0.003% by weight of the effect is almost no, the growth of the plant is less than 0.024% by weight.

이하, 본 발명을 더욱 상세하게 설명한다. Hereinafter, the present invention will be described in more detail.

본 발명 한약추출물을 이용한 식물병·해충 방제제의 한약재는 현재 한약상에서 쉽게 구할 수 있는 재료들로서 다음과 같다. 천문동, 빈랑자, 백부근, 천련자, 정향, 건강, 지유, 사군자, 지모, 대계, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑축, 포공영, 연교, 금은화, 여로, 건칠피, 용뇌, 연초엽의 26종으로 살균·살충·생리활성 작용을 나타내는 한약재로 구성된다. Herbal medicines of plant diseases and pest control agents using the herbal extracts of the present invention are as follows. Astronomical dong, Betel nut, Centennial muscle, Chunja, Clove, Health, Jiyu, Sagunja, Jimo, Daegye, Hakseul, Hwangbaekpi, Casualties, Kowloon, Gilgyeong, Hagocho, Lover, Governor, Black axis, Pogongyeong, Yeongyo, Geumeunhwa, Yeo, Gun It is composed of 26 kinds of chilpi, eruk, and tobacco leaves.

살균작용을 나타내는 한약재로는 건강(생강), 백부근(백부), 오배자(붉나무), 정향, 포공영(민들레), 금은화이며 인간에게는 건위제, 지혈제, 구강청정제, 해열제등으로 이용되고 있다. 또한, 살충작용을 나타내는 빈랑자, 사군자, 여로, 천련자(멀구슬), 학슬(담배풀), 사상자, 연초엽, 건칠피(옻나무), 용뇌(녹나무-장목)로 인간에게는 구충 및 회충, 혈압강화, 피부염 등의 치료에 이용되고 있다. 천문동, 지모, 대계, 황백피, 목통, 길경, 하고초, 애엽, 흑죽, 연교, 오배자는 보신 및 보양제로, 일반 사람들에게 널리 이용되고 있는 한약재로서 작물의 생리작용 촉진과 각종 병·해충으로부터의 저항성 증대를 위하여 조합하였다. Herbal medicines that exhibit bactericidal action are health (ginger), baekbugeun (baekbu), galleng (redwood), cloves, pogongyoung (dandelion), gold and silver, and is used in humans as a antagonist, hemostatic, mouthwash, and antipyretic. In addition, the insects exhibiting the insecticidal action, betel nut, dead man, young woman, mulberry (mulle), crane (cigarette), casualty, tobacco leaf, dried chile (lacquer tree), cedar (camphor-wood), insects and roundworms in humans, It is used for the treatment of blood pressure strengthening and dermatitis. Astronomical tract, Jimo, Daegye, Hwangbaekpi, Kukmok, Gilgyeong, Hagocho, Lobster, Black porridge, Yeongyo, Ginseng are bosin and nourishment, and are widely used by people in general, promoting the physiology of crops and resistance from various diseases and pests. Combined for augmentation.

이하 실시예에서 이용되는 본 발명 한약추출물을 이용한 식물병·해충 방제제의 제조방법은 아래와 같은 혼합, 추출, 정제의 3단계의 과정으로 제조된다.Method for producing a plant disease, pest control agent using the herbal extract of the present invention used in the following examples is prepared by the following three steps of mixing, extraction, purification.

혼합과정은 물 30리터를 기준량으로 천문동 300g중량, 빈랑자 300g중량, 백부근 300g중량, 천련자 300g중량, 정향 200g중량, 건강 200g중량, 지유 200g중량, 사군자 200g중량, 지모 200g중량, 대계 200g중량, 학슬 100g중량, 황백피 100g중량, 사상자 100g중량, 목통 100g중량, 길경 100g중량, 하고초 100g중량, 애엽 100g중량, 오배자 100g중량, 흑죽 100g중량, 포공영 100g중량, 연교 100g중량, 금은화 100g중량, 여로 100g중량, 건칠피 300g중량, 용뇌 300g중량, 연초엽 600g중량을 혼합한다. 추출과정은 혼합된 한약제 성분에 맥반석 가루를 리터당 1~10g중량을 넣고 약액이 충분히 빠져 나올수 있게 약탕기에 10시간 이상을 달여 추출하게 된다. 정제과정은 추출된 한약제 혼합물을 상온에서 충분히 식힌 후 추출액에 함유된 녹말 침전물을 제거하기 위해 맥반석 가루를 리터당 1~10g중량을 넣고 교반기를 이용하여 최소 1시간 이상을 교반하여 침전시킨다.The mixing process is based on 30 liters of water, 300g weight of astronomical dong, 300g weight of betel nut, 300g weight of bag, 300g weight of cinnamon, 200g weight of clove, 200g weight of fat, 200g weight of fat, 200g weight of sand, 200g weight of grand total, 200g weight , 100g weight of crane, 100g weight of Hwangbaek skin, 100g weight of casualty, 100g weight of neck, 100g weight of long neck, 100g weight of hagocho, 100g weight of young leaf, 100g weight of black gall, 100g weight of black porridge, 100g weight of poached young ginseng, 100g weight of gold bridge, 100g weight of silver coin, 100g by weight, 300g weight of dried skin, 300g weight of green onion, 600g weight of tobacco leaf. In the extraction process, 1 ~ 10g weight of ganban stone powder is put into the mixed Chinese medicine ingredients and the medicine is extracted for more than 10 hours in the shaker so that the medicine can be sufficiently drained. In the refining process, after cooling the extracted herbal mixture sufficiently at room temperature, in order to remove the starch precipitate contained in the extract solution, 1 to 10 g of weight of ganban rock powder is added and precipitated by stirring for at least 1 hour using an agitator.

이하, 본 발명 한약추출물을 이용한 식물병·해충 방제제의 식물에 대한 처리는 엽면처리와 관주처리 방법을 이용한다. 엽면처리는 원액을 1/500으로 희석하여 잘 섞은 다음 동력 및 인력분무기, 무인방제기 등을 이용하여 잎, 줄기, 과육등에 흐를 정도로 많게는 일주일에 1~3회 처리한다. 관주처리는 원액을 물에 1/1000~1/500으로 희석하여 잘 섞은 다음 점적관수 호스에 연결된 동력분무기 또는 동력 및 인력분무기를 이용하여 농작물의 뿌리 층까지 충분히 스며들 수 있게 처리하며 일주일에 1~3회 처리한다. Hereinafter, the treatment of plants of plant diseases and pest control agents using the herbal extract of the present invention uses foliar treatment and irrigation treatment. The foliar treatment is diluted 1/500 by diluting the stock solution, mixed well, and then processed once or three times a week so as to flow on leaves, stems, and flesh using power, manpower sprayers, and unmanned spraying machines. The irrigation treatment is dilute to 1/1000 ~ 1/500 in water, mix well, and then use the power sprayer or power sprayer connected to the drip irrigation hose to infiltrate the root layer of the crop sufficiently. ~ 3 times.

이하, 본 발명을 하기 실시예에 의거하여 상세히 설명하지만, 본 발명의 범위가 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail with reference to the following examples, but the scope of the present invention is not limited thereto.

실시예 1 : 본 발명 한약추출물의 딸기 흰가루병 방제효과Example 1: Strawberry powdery powder control effect of the present invention herbal extract

2002년 1월부터 2월까지 본 발명의 한약추출물을 이용하여 진주 산업대학교 부설 식물클리닉센터에 딸기 흰가루병 방제효과 실증시험을 의뢰하여 실시하였다. 실시 장소는 김해 생림지역 딸기 흰가루병 발생농가에서 이루어졌다. 본 발명의 한약추출물 원액을 1/500으로 희석하여 감염된 딸기의 엽, 줄기, 과육에 충분히 흘러내릴 정도로 처리하였고, 대조 약제로는 살균제인 훼나리몰 수화제 공시배액(1/3,000)으로 희석하여 같은 방법으로 처리하였다. 본 실시는 3번 반복하여 이루어졌고, 시용 및 검증은 발병초기부터 7일 간격으로 3회 살포한 다음 마지막 처리 후 15일 뒤 실증조사가 이루어 졌으며, 농촌진흥청 공정시험법(1996)에 의해 검증되었다. From January to February 2002, using the herbal extract of the present invention was commissioned to test the effect of controlling strawberry powdery powder to the plant clinic center attached to Jinju Industrial University. The project was conducted at a farm where strawberry powdery mildew disease occurred in the Gimhae forest area. The herbal extract of the present invention was diluted to 1/500 and treated enough to flow down to the leaves, stems, and pulp of the infected strawberry, and the control agent was diluted with the fecal funeral molar hydrate (1 / 3,000). It was processed by the method. The test was repeated three times, and application and verification were applied three times at intervals of seven days from the beginning of the onset, and then 15 days after the last treatment, the empirical investigation was conducted, and was verified by the RDA Process Test Method (1996). .

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본 발명 한약추출물의 딸기에 대한 약해Weakness against strawberry of the present invention herbal extract 약 해 정 도(0~5)About solution (0 ~ 5) 희석율Dilution rate 기준량(1/500)Reference amount (1/500) 배량(1/250)Displacement (1/250) 00 00 [주]진주 산업대학교 부설 식물클리닉센터(2002.02)품종; 장희(아키히메)방법; 처리 15일 후 육안조사Varieties of Plant Clinic Center, Jinju Industrial University (2002.02); Janghee (Akihime) method; Visual inspection 15 days after treatment

도 1에서 보는 바와 같이, 본 발명 한약추출물을 이용한 딸기 흰가루병 방제효과 측정실험에서, 무 처리구 대비 본 발명 한약추출물 92%, 본 발명 한약추출물과 농약혼용 90%, 농약단독 64%의 방제효과를 나타내어, 본 발명 한약추출물의 살균에 대한 효과가 매우 우수한 것으로 검증되었다. 한편 약해는 기준량 및 배량시험에서 전혀 나타나지 않았다(표 1 참조). As shown in Figure 1, in the measurement of strawberry powdery mildew control effect using the present invention herbal extracts, 92% of the inventive herbal extracts, 90% of the inventive herbal extracts and pesticide mixture 90%, pesticides alone 64% compared to the untreated group In addition, the effect of the present invention on the sterilization of the herbal extract was proved to be very excellent. On the other hand, the weakness did not appear at all in the baseline and batch tests (see Table 1).

실시예 2 : 본 발명 한약추출물의 고추 흰가루병 방제효과Example 2 Control Effect of Red Pepper Powder Powder of the Herbal Medicine Extract of the Present Invention

2002년 2월부터 3월까지 본 발명의 한약추출물을 이용하여 진주 산업대학교 부설 식물클리닉센터에서 고추 흰가루병 방제효과를 확인하기 위한 실증시험이 수행되었다. 장소는 진주 산업대 원예시원장에서 이루어졌다. 본 발명의 한약추출물 원액을 1/500로 희석하여 감염된 고추의 엽에 충분히 흘러내릴 정도로 처리하였고, 대조 약제로는 살균제인 훼나리몰 수화제 공시배액(1/3,000)으로 희석하여 같은 방법으로 처리하였다. 본 실시는 3회 반복으로 이루어졌고, 시용 및 검증은 발병 초기부터 7일 간격으로 3회 살포한 다음 마지막 처리 후 15일 뒤 실증조사가 이루어 졌으며, 농촌진흥청 공정시험법(1996)에 의해 검증되었다. From February to March 2002, an empirical test was conducted to confirm the effect of controlling pepper powdery mildew at the Plant Clinic Center affiliated with Jinju Industrial University using the herbal extract of the present invention. The place was at the Pearl Gardening Director. The herbal extract of the present invention was diluted to 1/500 and treated enough to flow down the leaves of the infected pepper, and the control agent was diluted with fenaminary hydrate wetting solution (1 / 3,000) and treated in the same manner. . The test was repeated three times, and application and verification were applied three times at the interval of seven days from the beginning of the onset, and then 15 days after the last treatment, the empirical investigation was conducted, and was verified by the RDA Process Test Method (1996). .

본 발명 한약추출물의 고추 흰가루병에 대한 효과(이병엽율 %)Effect of the Herbal Medicine Extracts of Red Pepper Powder Powder on Disease 처리약제Treatment Pharmaceutical 희석율Dilution rate 이병엽율(%)Lesion rate (%) DMRT(5%)DMRT (5%) 방제가(%)Control price (%) 1회1 time 2회Episode 2 3회3rd time 평균Average 본발명한약추출물Herbal extract of the present invention 1/5001/500 12.012.0 18.118.1 7.37.3 12.512.5 aa 86.986.9 본말명한약추출물+훼나리몰Korean herbal medicine extract + fenarimol 1/500(본발명)+1/6000(훼나리몰)1/500 (invention) + 1/6000 (Fenari Mall) 1.61.6 0.00.0 6.36.3 2.62.6 aa 97.397.3 훼나리몰Feminari mall 1/30001/3000 0.00.0 9.09.0 0.90.9 3.53.5 aa 96.396.3 무처리No treatment -- 97.797.7 99.999.9 89.989.9 95.595.5 bb 00 CV 16.1%CV 16.1% [주]진주 산업대학교 부설 식물클리닉센터 (2002.03)품종; 고추(복광)방법; 7일 간격 3회 처리 한 다음 15일 후Varieties of Plant Clinic Center, Jinju Industrial University (2002.03); Red pepper method; 3 treatments every 7 days, then 15 days

본 발명 한약추출물의 고추 흰가루병에 대한 효과(발병율 %)Effect of the herb extract on red pepper powder (incidence rate%) 처리약제Treatment Pharmaceutical 희석율Dilution rate 발병율(%)Incidence (%) DMRT(5%)DMRT (5%) 방제가(%)Control price (%) 1회1 time 2회Episode 2 3회3rd time 평균Average 본발명한약추출물Herbal extract of the present invention 1/5001/500 3.53.5 4.54.5 3.03.0 3.73.7 aa 95.695.6 본말명한약추출물+훼나리몰Korean herbal medicine extract + fenarimol 1/500(본발명)+1/6000(훼나리몰)1/500 (invention) + 1/6000 (Fenari Mall) 2.32.3 0.00.0 0.70.7 1.11.1 aa 98.798.7 훼나리몰Feminari mall 1/30001/3000 0.00.0 0.30.3 4.34.3 1.51.5 aa 98.298.2 무처리No treatment -- 83.183.1 85.685.6 83.283.2 83.983.9 bb 00 CV 7.9%CV 7.9% [주]진주 산업대학교 부설 식물클리닉센터 (2002.03)품종; 고추(복광)방법; 7일 간격 3회 처리 한 다음 15일 후Varieties of Plant Clinic Center, Jinju Industrial University (2002.03); Red pepper method; 3 treatments every 7 days, then 15 days

본 발명 한약추출물의 고추에 대한 약해Weakness against red pepper of the present invention herbal extract 본 발명 한약추출물의 고추에 대한 약해정도(0~5)Degree of weakness against red pepper of the present invention herbal extract (0 ~ 5) 희석율Dilution rate 기준량(1/500)Reference amount (1/500) 배량(1/250)Displacement (1/250) 00 00 [주]진주 산업대학교 부설 식물클리닉센터 (2002.03)품종; 고추(복광)방법; 처리 15일 후 육안조사Varieties of Plant Clinic Center, Jinju Industrial University (2002.03); Red pepper method; Visual inspection 15 days after treatment

표 2 내지 표 4에서 보는 바와 같이, 고추에 발생되는 흰가루병에 본 발명 한약추출물, 본 발명 한약추출물+훼나리몰(살균제), 훼나리몰의 이병엽율(%) 방제가는 각각 무처리구 대비 86.9%, 97.3%, 96.3%(DMRT-5%, 유의차-16.1%)로 살균제 농약과 같은 방제가를 나타내었다. 그리고 발병도(%)에서는 각각 무처리구 대비 95.6%, 98.7%, 98.2%(DMRT-5%, 유의차-7.9%)의 억제를 보여 공시농약과 대등한 효과가 입증되었으며, 기준량 및 배량에 대해 전혀 약해를 나타내지 않았다. As shown in Table 2 to Table 4, the herbal medicine extract of the present invention, the herbal extract of the present invention + fenarimol (bactericide), feminar morbidity (%) control of the powdery mildew disease caused by pepper is 86.9%, respectively, compared to the untreated group, 97.3% and 96.3% (DMRT-5%, significant difference -16.1%) showed the same control value as fungicide pesticide. Also, the incidence (%) showed 95.6%, 98.7%, and 98.2% (DMRT-5%, significant difference -7.9%) of inhibition compared to the non-treated, respectively, and proved to be comparable to the public pesticides. It did not show any weakness.

실시예 3 : 잎들깨의 진딧물 및 응애에 대한 방제효과Example 3 Control Effect on Aphids and Mites of Leafy Perilla

2002년 3월부터 4월까지 본 발명의 한약추출물을 이용하여 진주 산업대학교 부설 식물클리닉센터에 의해 잎들깨의 주요 가해 해충인 목화진딧물, 점박이응애에 대한 방제효과 실증시험이 실시되었다. 실증시험 장소는 경남 진주시에서 지정한 진주시 명석면 외율리 들깻잎 수출지정 비닐하우스 농가에서 이루어졌다. 본 실시는 본 발명 한약추출물 원액을 1/500로 희석하여 감염된 들깻잎의 엽에 충분히 흘러내릴 정도로 처리하였고, 대조 약제로는 살충제인 코니도 수화제 공시배액(1/2,000)으로 희석하여 같은 방법으로 처리하였다. 본 실시는 3회 반복으로 이루어졌으며, 농촌진흥청 공정시험법(1996)에 의거 검증은 발생초기 1회 처리 후 5일 간격으로 약효를 조사하였으며, 처리 15일 경과 후 기준량 및 배량에 대한 약해를 조사하였다. 그리고 실내 실시를 통해 진딧물 종류별(목화진딧물, 복숭아혹진딧물) 사충율을 시험을 실시하였다. From March to April 2002, the herbal medicine extract of the present invention was used by the Plant Clinic Center affiliated with Jinju National University of Korea to demonstrate the control effect against cotton aphid and spotted mite, which are the major pests of perilla. The empirical test site was held at a farmhouse designated for export of perilla leaves in Oyeol-ri, Myeongseok-myeon, Jinju-si, Gyeongju. In this embodiment, the herbal extract of the present invention was diluted to 1/500 and treated to the extent that the leaf of infected perilla leaf was sufficiently flowed, and the control agent was diluted with the contaminant hydration test solution (1 / 2,000), which is an insecticide, and treated in the same manner. It was. This practice was repeated three times, and verification was conducted by the Rural Development Administration's Process Test Method (1996) every five days after the initial treatment. It was. And the mortality rate by aphid type (cotton aphid, peach aphid) was tested through indoors.

본 발명 한약추출물의 목화진딧물에 대한 방제효과Control Effect of the Herbal Extract of the Invention on Cotton Aphid 처리약제Treatment Pharmaceutical 희석율Dilution rate 처리전 밀도Density before treatment 방제율(%)Control rate (%) 약제처리 5일 후5 days after treatment 약제처리 10일 후10 days after treatment 약제처리 15일 후15 days after treatment 본발명한약추출물Herbal extract of the present invention 1/5001/500 18.218.2 79.979.9 85.385.3 90.390.3 코니도Conido 1/20001/2000 18.818.8 100.0100.0 100.0100.0 100.0100.0 무처리No treatment -- 19.519.5 00 00 00 [주]진주 산업대학교 부설 식물클리닉센터품종; 잎들깨(만추)방법; 발생초기 1회 처리 후 5일 간격으로 실증조사해충; 목화진딧물, 점박이응애Varieties of Plant Clinic Center, Jinju National University; Leaf perilla (full leaf) method; Empirical pests at 5 day intervals after treatment at the beginning of development; Cotton aphid, spotted mite

본 발명 한약추출물의 점박이응애에 대한 방제효과Control Effect on Spotted Mites of the Herbal Extracts of the Present Invention 처리약제Treatment Pharmaceutical 희석율Dilution rate 처리전 밀도Density before treatment 방제율(%)Control rate (%) 약제처리 5일 후5 days after treatment 약제처리 10일 후10 days after treatment 약제처리 15일 후 15 days after treatment 본발명한약추출물Herbal extract of the present invention 1/5001/500 237237 82.382.3 70.770.7 60.360.3 코니도Conido 1/20001/2000 230230 83.983.9 89.189.1 75.975.9 무처리No treatment -- 232232 00 00 00 [주]진주 산업대학교 부설 식물클리닉센터품종; 잎들깨(만추)방법; 발생초기 1회 처리 후 5일 간격으로 실증조사해충; 목화진딧물, 점박이응애Varieties of Plant Clinic Center, Jinju National University; Leaf perilla (full leaf) method; Empirical pests at 5 day intervals after treatment at the beginning of development; Cotton aphid, spotted mite

본 발명 한약추출물의 약해시험Weakness test of herbal extract of the present invention 약해정도Weakness 희석율Dilution rate 기준량(1/500)Reference amount (1/500) 배량(1/250)Displacement (1/250) 00 00 [주]진주 산업대학교 부설 식물클리닉센터품종; 잎들깨(만추)방법; 처리 15일 후 육안조사Varieties of Plant Clinic Center, Jinju National University; Leaf perilla (full leaf) method; Visual inspection 15 days after treatment

표 5, 6은 들깻잎의 목화진딧물, 점박이응애에 대해 본 발명 한약추출물과 코니도(살충제)에 대한 실험결과이다. 목화진딧물의 방제가는 처리 15일후 본 발명 한약추출물 90.3%, 살충제 100%, 점박이응애는 약제처리 5일후 본 발명 한약추출물 82.3%, 살충제 83.9%로써 농약과 같은 대등한 방제가를 나타내었다. 그리고 들깻잎에 대한 약해시험 결과 표 7에서 보듯이 기준량(500배액)과 배량(250배액)에서 전혀 약해 증상이 나타나지 않았다. Tables 5 and 6 show the results of the inventive herbal extracts and condo (insecticides) on cotton aphids and spotted mites of perilla leaves. After controlling the cotton aphid 15 days after the treatment of the invention herbal extract 90.3%, insecticide 100%, spot mite showed a comparable control value, such as pesticides as 82.3%, herbal pesticide 83.9% the invention 5 days after treatment. As a result of the test of perilla leaves, as shown in Table 7, there was no sign of weakness at all in the standard dose (500 times) and the double dose (250 times).

본 발명 한약추출물의 처리시간에 따른 목화진딧물의 살충효과(실내실험)Insecticidal effect of cotton aphids according to treatment time of herbal extracts of the present invention (indoor experiment) 처리약제Treatment Pharmaceutical 희석율Dilution rate 방제율(%)Control rate (%) 30분 후30 minutes later 1시간 후1 hour later 4시간 후4 hours later 6시간 후6 hours later 본발명 한약추출물Herbal Extract of the Invention 1/5001/500 100100 98.598.5 100100 99.499.4 무처리No treatment -- 00 00 00 00 [주]진주 산업대학교 부설 식물클리닉센터(2002.04)해충; 목화진딧물, 복숭아혹진딧물[Plant] Clinic Center, Jinju Industrial University (2002.04) Pest; Cotton aphid, peach aphid

본 발명 한약추출물의 처리시간에 따른 복숭아혹진딧물의 살충효과(실내실험)Insecticidal effect of peach aphids according to the treatment time of the herbal extract of the present invention (indoor experiment) 처리약제Treatment Pharmaceutical 희석율Dilution rate 방제율(%)Control rate (%) 1시간 후1 hour later 2시간 후2 hours later 6시간 후6 hours later 본발명 한약추출물Herbal Extract of the Invention 1/5001/500 62.562.5 62.462.4 59.359.3 무처리No treatment -- 00 00 00 [주]진주 산업대학교 부설 식물클리닉센터(2002.04)해충; 목화진딧물, 복숭아혹진딧물[Plant] Clinic Center, Jinju Industrial University (2002.04) Pest; Cotton aphid, peach aphid

표 8, 9는 본 발명 한약추출물 제제 처리 후 처리시간에 따른 목화진딧물과 복숭아혹진딧물에 대한 살충효과 검증자료로써, 목화진딧물의 경우 처리 30분 후부터 100%의 방제율을 나타내었다.Tables 8 and 9 show the insecticidal effect of cotton aphids and peach aphids according to the treatment time after treatment of the herbal extract of the present invention, and 100% of cotton aphids were treated after 30 minutes of treatment.

실시예 4 : 오이 흰가루병 방제효과Example 4 Cucumber Powdery Disease Control Effect

2002년 4월부터 6월까지 본 발명의 한약추출물을 이용하여 진주 산업대학교 부설 식물클리닉센터에 의해 오이 흰가루병에 대한 방제효과 실증시험이 수행되었다. 실증시험 장소는 진주 산업대 원예시원장에서 이루어 졌으며, 본 발명 한약추출물 원액을 1/500으로 희석하여 감염된 오이의 엽에 충분히 흘러내릴 정도로 처리하였고, 대조 약제로는 살균제인 훼나리몰 수화제 공시배액(1/3,000)으로 희석하여 같은 방법으로 처리하였다. 이는 3회 반복하여 실시되었고, 시용 및 검증은 발병초기부터 7일 간격으로 3회 살포한 다음 마지막 처리 후 15일 뒤 실증조사가 이루어 졌으며, 농촌진흥청 공정시험법(1996)에 의해 검증되었다. From April to June 2002, using the herbal extract of the present invention, a demonstration test for control of cucumber powdery mildew was carried out by the Plant Clinic Center attached to Jinju Industrial University. The empirical test site was made by the horticultural director of the Pearl Industrial University, and the crude herbal extract of the present invention was diluted to 1/500 and treated enough to flow down to the leaves of infected cucumbers. 1 / 3,000) and treated in the same way. This was repeated three times, and application and verification were applied three times at intervals of seven days from the beginning of the onset, and then 15 days after the last treatment.

본 발명 한약추출물의 오이 흰가루병에 대한 방제효과(발병율%)Control Effect of Cucumber Powdery Mildew Extract of the Herbal Medicine of the Invention (Incidence%) 처리약제Treatment Pharmaceutical 희석율Dilution rate 발병율(%)Incidence (%) DMRTDMRT 방제율(%)Control rate (%) 1회1 time 2회Episode 2 3회3rd time 평균Average 본발명 한약추출물Herbal Extract of the Invention 1/5001/500 12.812.8 12.612.6 22.322.3 15.915.9 aa 80.080.0 본발명한약추출물+훼나리몰Herbal Medicine Extract + Feenari Mall 1/500(본발명)+1/6000(훼나리몰)1/500 (invention) + 1/6000 (Fenari Mall) 5.55.5 3.53.5 7.07.0 5.35.3 aa 93.393.3 훼나리몰Feminari mall 1/30001/3000 5.45.4 2.42.4 8.38.3 5.45.4 aa 93.193.1 무처리No treatment -- 74.274.2 81.781.7 80.080.0 78.678.6 bb 00 [주]진주 산업대학교 부설 식불클리닉센터(2002.06)품종; 일향청장오이방법; 발생초기 1회 처리 후 5일 간격으로 실증조사해충; 목화진딧물, 점박이응애Varieties of Food and Drink Clinic Center (2002.06) affiliated with Jinju National University; Iyang Cheongjang Cucumber Method; Empirical pests at 5 day intervals after treatment at the beginning of development; Cotton aphid, spotted mite

오이에 대한 본 발명 한약추출물의 약해Weakness of the Invention Herbal Extract of the Invention on Cucumber 약해정도(0~5)Weakness (0 ~ 5) 희석율Dilution rate 기준량(1/500)Reference amount (1/500) 배량(1/250)Displacement (1/250) 00 00 [주]진주 산업대학교 부설 식물클리닉센터(2002.06)품종; 일향청장오이방법; 처리 15일 후 육안조사Varieties of Plant Clinic Center, Jinju Industrial University (2002.06); Iyang Cheongjang Cucumber Method; Visual inspection 15 days after treatment

표 10, 11은 오이의 흰가루병에 대해 본 발명 한약추출물과 훼나리몰수화제(살균제)에 대한 방제효과 및 약해 실험결과이다. 본 발명 한약추출물, 본 발명 한약추출물+훼나리몰(살균제), 훼나리몰의 방제가는 각각 무처리구 대비 80.0%, 93.3%, 93.1%(DMRT-5%, 유의차-17.5%)로 살균제 농약과 대등한 방제가를 나타내었다. 그리고 기준량 및 배량에 대해 전혀 약해를 나타내지 않았다. Tables 10 and 11 show the results of the control and weakening experiments of the inventive herbal extracts and the fungal dehydrating agent (bactericide) on the powdery mildew of cucumber. The herbal extracts of the present invention, the herbal extracts of the present invention + fenarimol (bactericide) and the control of the fenarimol are 80.0%, 93.3%, 93.1% (DMRT-5%, significant difference -17.5%) compared to the untreated group, respectively, The equivalent control price was shown. And no harm to baseline and dose.

실시예 5 : 잔디에 대한 비효 및 약해시험Example 5 Inactivation and Harmfulness Tests on Turfgrasses

본 실시는 상주대학교 농과대학 농업과학연구소에서 본 발명 한약추출물(시험명: HJ-001)의 생장촉진효과 및 약해시험이 수행되었다. 경북 대구 경산골프장에서 공시잔디인 라이그라스(Lolium perenne 'Paragon')에 대해 2001년 9월부터 2002년 2월까지 이루어졌으며, 처리내용 및 시험방법은 다음과 같다. 농업과학기술원 분석에 따른 생장촉진효 및 약해에 대한 분석법은 아래와 같다. 이하, 본 실시예의 표에서 본 발명 한약추출물은 HJ-001로 기재한다.In this experiment, growth promoting effect and weakening test of the herbal extracts of the present invention (test name: HJ-001) were conducted at the Institute of Agricultural Sciences, College of Agriculture, Sangju National University. Lolium perenne 'Paragon', a grass lawn, was made from September 2001 to February 2002 at the Gyeongsan Golf Course in Daegu, Gyeongbuk. The treatment contents and test methods are as follows. The analysis method for growth promoting effect and weakness according to the analysis of the Agricultural Science and Technology Institute is as follows. Hereinafter, the herbal extract of the present invention in the table of the present embodiment is described as HJ-001.

실시예 5의 처리내용Processing contents of example 5 처리약제Treatment Pharmaceutical 비효Ineffective 비해Than 시험연차Annual examination 10a당 사용량Usage per 10a 처리시기 및 방법When and how to process 기준량(1/500)Reference amount (1/500) 배량(1/250)Displacement (1/250) 본발명한약추출물실험구(HJ-011처리)The invention herbal extract experimental zone (HJ-011 treatment) 2L2L 발아 2~3일 후 공시비료를 고루 살포Evenly spread the fertilizer after 2 ~ 3 days of germination 2L2L 4L4L 1One 대조구(무처리)Control (no treatment) -- -- -- -- --

실시예5의 실험구 배치 및 면적Experimental Arrangement and Area of Example 5 구분division 처리수Treated water 반복수Iterations 총구수Muzzle 구당 면적Ward area 시험구 면적Test Area 총면적Total area 생장촉진효Growth promoting effect 22 33 66 5m2 5 m 2 30m2 30 m 2 60m2 60 m 2 약해Weak 22 33 66 5m2 5 m 2 30m2 30 m 2

토양분석Soil analysis

그린용 홀컵기(직경-11cm)를 이용하여 15cm 깊이로 처리 전 토양을 9월 1일 채취한 다음, 10월 1일과 10월 30일에는 처리 후 토양을 채취한 뒤 잔디를 들어내고 3반복의 시료를 혼합하여 건조한 후 일정량을 분석용 시료로 사용하였다. 그리고 pH, EC, 치환성 양이온(K, Ca, Mg) O.M. 유효인산, 유효규산, Fe, Mn, B, Mo, Pb, Cd, Cr, Ni, Ti 분석하였다.Using green hole cup machine (11cm in diameter), the soil was collected to the depth of 15cm before September 1, and on October 1 and October 30 after the treatment, the soil was collected and the grass was lifted. After mixing and drying the sample, a certain amount was used as the sample for analysis. And pH, EC, substitutional cations (K, Ca, Mg) O.M. Effective phosphoric acid, effective silicic acid, Fe, Mn, B, Mo, Pb, Cd, Cr, Ni, Ti were analyzed.

상기 처리 전의 토양분석의 결과와 처리 후 토양의 처리구 별 토양화학성 변화를 표 14, 15, 16에 나타내었다. The results of the soil analysis before the treatment and the soil chemical change of the treated soil after treatment are shown in Tables 14, 15 and 16.

처리 전 토양분석 결과Soil analysis results before treatment pHpH EC1:5(dS/m)EC 1: 5 (dS / m) OM(%)OM (%) Av.-P2O5 Av.-P 2 O 5 Av.-SiO2 Av.-SiO 2 Ex.-cation(me/100g)Ex.-cation (me / 100g) Av.-BAv.-B 0.1N-HCl extraction(mg/kg)0.1N-HCl extraction (mg / kg) mg/kgmg / kg mg/kgmg / kg KK CaCa MgMg mg/kgmg / kg FeFe MnMn BB MoMo PbPb CdCD CrCr TiTi NiNi 6.76.7 0.150.15 1.861.86 54.754.7 218.5218.5 0.080.08 2.692.69 0.430.43 0.100.10 0.400.40 57.757.7 0.140.14 0.010.01 0.300.30 0.030.03 0.090.09 0.480.48 0.330.33

본 발명 한약추출물 처리구 별 토양화학성 변화(10월 1일)Soil Chemical Changes of Herbal Extracts Treated with Invention (October 1) 처리process pHpH EC1:5(dS/m)EC 1: 5 (dS / m) OM(%)OM (%) Av.-P2O5 Av.-P 2 O 5 Av.-SiO2 Av.-SiO 2 Ex.-cation(me/100g)Ex.-cation (me / 100g) Av.-BAv.-B 0.1N-HCl extraction(mg/kg)0.1N-HCl extraction (mg / kg) mg/kgmg / kg mg/kgmg / kg KK CaCa MgMg mg/kgmg / kg FeFe MnMn BB MoMo PbPb CdCD CrCr TiTi NiNi 대조구Control 7.27.2 0.050.05 1.901.90 53.653.6 153.1153.1 0.090.09 2.792.79 0.420.42 0.020.02 0.200.20 52.152.1 0.110.11 0.010.01 0.330.33 0.330.33 0.060.06 0.870.87 0.540.54 본발명HJ-001The present invention HJ-001 7.27.2 0.050.05 1.241.24 56.256.2 193.0193.0 0.080.08 2.922.92 0.370.37 0.580.58 0.200.20 52.552.5 0.160.16 0.020.02 0.300.30 0.030.03 0.060.06 0.480.48 0.510.51

본 발명 한약추출물 처리구 별 토양화학성 변화(10월 30일)Soil Chemical Changes of Herbal Extracts Treated with Invention (October 30) 처리process pHpH EC1:5(dS/m)EC 1: 5 (dS / m) OM(%)OM (%) Av.-P2O5 Av.-P 2 O 5 Av.-SiO2 Av.-SiO 2 Ex.-cation(me/100g)Ex.-cation (me / 100g) Av.-BAv.-B 0.1N-HCl extraction(mg/kg)0.1N-HCl extraction (mg / kg) mg/kgmg / kg mg/kgmg / kg KK CaCa MgMg mg/kgmg / kg FeFe MnMn BB MoMo PbPb CdCD CrCr TiTi NiNi 대조구Control 7.27.2 0.040.04 1.301.30 69.569.5 122.6122.6 0.090.09 3.043.04 0.380.38 0.080.08 0.200.20 44.644.6 0.150.15 0.010.01 0.270.27 0.030.03 0.060.06 0.480.48 0.270.27 본발명HJ-001The present invention HJ-001 7.27.2 0.050.05 1.861.86 67.867.8 219.6219.6 0.080.08 3.243.24 0.410.41 0.400.40 0.310.31 42.642.6 0.190.19 0.020.02 0.270.27 0.040.04 0.060.06 0.360.36 0.300.30

식물체분석Plant Analysis

상기와 같은 방법으로 채취하고 처리한 후 P, K, Ca, Mg, Fe, B, Mo, Mn, T-N을 분석하였다. 그 결과, 식물체 분석 결과를 표 17, 18에 나타내었다.    P, K, Ca, Mg, Fe, B, Mo, Mn, T-N was analyzed after harvesting and processing in the same manner as described above. As a result, the plant analysis results are shown in Tables 17 and 18.

본 발명 한약추출물 후 식물체 분석결과(시료채취:10월 1일)Plant analysis result after the herbal extract of the present invention (sample collection: October 1) 처리process T-NT-N PP KK CaCa MgMg FeFe MnMn BB MoMo (%)(%) (mg/kg)(mg / kg) 대조구Control 3.223.22 1.721.72 3.853.85 0.920.92 0.320.32 265.2265.2 126.0126.0 17.617.6 0.170.17 본발명HJ-001The present invention HJ-001 3.403.40 1.841.84 3.913.91 0.750.75 0.360.36 346.2346.2 125.5125.5 18.918.9 0.200.20

본 발명 한약추출물 처리 후 식물체 분석결과(시료채취:10월 30일)Analysis of plant after treatment with herbal extracts of the present invention (sample collection: October 30) 처리process T-NT-N PP KK CaCa MgMg FeFe MnMn BB MoMo (%)(%) (mg/kg)(mg / kg) 대조구Control 3.423.42 0.590.59 3.363.36 0.790.79 0.320.32 480.5480.5 165.4165.4 18.118.1 0.160.16 본발명HJ-001The present invention HJ-001 3.693.69 0.740.74 2.652.65 0.960.96 0.360.36 496.5496.5 194.3194.3 20.220.2 0.190.19

식물생육Plant growth

10월 1일 그린 홀컵기(직경-11cm)를 사용하여 15cm 깊이로 잔디를 채취한 후 흙에서 분리하여 지상부와 지하부 길이를 조사하였고, 조사 후 일반적인 한지형 잔디 관리에 준하여 27mm 높이로 깍아 준 다음 10월 30일 지상부와 지하부 길이를 재조사하였다. 그 결과 식물체의 지상부 생장과 지하부 생장을 표 21, 22, 23, 24에 나타내었다.On October 1, using a green hole cup machine (-11cm diameter), grass was collected to a depth of 15cm and separated from the soil to investigate the length of the ground and underground parts. On June 30, the ground and underground lengths were reviewed. As a result, the above-ground and underground growth of the plants are shown in Tables 21, 22, 23, and 24.

본 발명 한약추출물 처리 30일 후 지상부 생장Growth of above-ground part after 30 days of treatment with herbal extracts of the present invention 처리process 지상부 길이(cm)Ground length (cm) 유의차(LSD)Significant difference (LSD) 1회1 time 2회Episode 2 3회3rd time 평균Average 대조구Control 7.47.4 7.97.9 7.87.8 7.77.7 aa 본발명HJ-001The present invention HJ-001 7.47.4 7.17.1 7.17.1 7.27.2 aa CV 2.89%CV 2.89%

본 발명 한약추출물 처리 30일 후 지하부 생장Underground growth after 30 days of treatment with herbal extracts of the present invention 처리process 지하부 길이(cm)Underground length (cm) 유의차(LSD)Significant difference (LSD) 1회1 time 2회Episode 2 3회3rd time 평균Average 대조구Control 5.45.4 5.45.4 4.54.5 5.15.1 aa 본발명HJ-001The present invention HJ-001 6.56.5 5.95.9 5.85.8 6.16.1 aa CV 8.14%CV 8.14%

본 발명 한약추출물 처리 60일 후 지상부 생장60 days after the treatment of the present invention 처리process 지상부 길이(cm)Ground length (cm) 유의차(LSD)Significant difference (LSD) 1회1 time 2회Episode 2 3회3rd time 평균Average 대조구Control 9.59.5 10.010.0 10.010.0 9.89.8 aa 본발명HJ-001The present invention HJ-001 11.011.0 10.510.5 11.211.2 10.910.9 aa CV 3.15%CV 3.15%

본 발명 한약추출물 처리 60일 후 지하부 생장Underground growth after 60 days treatment of the herbal extract of the present invention 처리process 지하부 길이(cm)Underground length (cm) 유의차(LSD)Significant difference (LSD) 1회1 time 2회Episode 2 3회3rd time 평균Average 대조구Control 7.07.0 8.08.0 6.86.8 7.37.3 aa 본발명HJ-001The present invention HJ-001 8.08.0 9.09.0 9.09.0 8.68.6 aa CV 7.67%CV 7.67%

약해Weak

10월 1일, 10월 30일, 11월 30일에 처리구 당 100주를 선정하여 식물체에 나타나는 증상을 달관 조사하였다. 그 결과를 표 25에 나타내었다.100 weeks per treatment group were selected on October 1, October 30, and November 30, and the symptoms on the plants were examined. The results are shown in Table 25.

본 발명 한약추출물 처리 30일, 60일, 90일 후의 약해조사Weak investigation after 30 days, 60 days, 90 days 처리process 공시작물Blank 약해정도(0~5)Weakness (0 ~ 5) 30일 후30 days later 60일 후60 days later 90일 후90 days later 2mL/m2 2 mL / m 2 4mL/m2 4 mL / m 2 2mL/m2 2 mL / m 2 4mL/m2 4 mL / m 2 2mL/m2 2 mL / m 2 4mL/m2 4 mL / m 2 본발명HJ-001The present invention HJ-001 라이그라스Ryegrass 00 00 00 00 00 00

본 발명 한약추출물을 이용하여 라이그라스(한지형잔디)에 대한 생장촉진효 및 약해 시험에 대한 토양분석 결과 토양 내 가용성 붕소와 몰리브덴의 함량은 증가된 반면 토양 내 가용성 중금속 함량의 증가현상은 나타나지 않았다. 그리고 식물체 분석결과 본 발명 한약추출물 처리 시 생육초기에는 식물체의 인산, 가리, 철, 붕소, 몰리브덴의 함량을 증가 시켰고, 생육후기에는 대조구에 비해 모든 분석항목에서 함량이 증가되는 경향이 두드러지게 나타났다. HJ-001의 시용은 라이그라스의 지상부와 지하부 생장을 촉진시켰으며, 기준량(2mL/㎡), 배량(4mL/㎡) 처리시 90일째까지 전혀 비해가 발생되지 않음으로써 본 발명 한약추출물이 작물에 대한 안전성 및 생장촉진제로서도 우수한 물질임이 검증되었다. As a result of soil analysis for growth promoting effect and weakening test for lygras (Hanji grass) using the herbal extract of the present invention, the content of soluble boron and molybdenum was increased, but there was no increase of soluble heavy metal content in soil. As a result of the analysis of the plant, when the herbal extract of the present invention was treated, the content of phosphate, gal, iron, boron, and molybdenum of the plant was increased in the early stage of growth. The application of HJ-001 promoted the growth of the ground and underground parts of lygras, and the herbal medicine extract of the present invention did not occur at 90 days after treatment with the standard amount (2mL / ㎡) and doubling (4mL / ㎡). It has been proved to be an excellent material as a safety and growth promoter.

상기 살펴본 것과 같이, 본 발명의 한약추출물을 이용한 식물병·해충 방제제는 살균·살충효과와 더불어 생장촉진효과를 동시에 가지고 있을 뿐만 아니라, 인간이 사용중인 천연한방제로 만들어져 있기 때문에 인축에 안전하다는 가장 큰 장점을 가지고 있으므로, 이를 이용한 각종 작물의 환경 친화적 관리 및 국민의 건강 증진은 물론 화학농약의 대체 제제로서 국가 경쟁력 향상에도 기여할 것이다. As described above, plant diseases and pest control agents using the herbal extracts of the present invention not only have a bactericidal and insecticidal effect, but also have a growth promoting effect, and are made of natural herbal medicines that are being used by humans. As it has great advantages, it will contribute to the improvement of national competitiveness as an alternative to chemical pesticides as well as environmentally friendly management of various crops and health of the people.

이상에서 설명한 바와 같이, 본 발명 한약추출물을 이용한 식물병·해충 방제제는 살균·살충의 효과가 뛰어날 뿐만 아니라 식물에 약해를 입히지 않고 오히려 그 생장을 촉진시키는 효과를 가지고 있어서, 화학농약의 우수한 대체 제제로 사용될 수 있으므로 농약 산업상 매우 유용한 발명이다.As described above, the plant diseases and pest control agents using the herbal extracts of the present invention not only have excellent sterilization and insecticidal effects, but also do not cause harm to plants, but rather promote their growth, and thus are excellent substitutes for chemical pesticides. It can be used as a formulation and is a very useful invention for the pesticide industry.

도 1은 본 발명 한약추출물의 딸기 흰가루병의 방제효과를 나타낸 그래프이다. 1 is a graph showing the control effect of strawberry powdery mildew of the present invention.

Claims (3)

한약재를 물과 혼합하는 단계; 상기 혼합물을 약탕기에서 10시간 이상 달여서 추출하는 단계; 상기 추출물에 맥반석 가루를 넣고 교반한 후 침전시켜 여과하는 단계를 포함하는 한약추출물을 이용한 식물병·해충 방제제의 제조방법에 있어서,Mixing the herbal medicine with water; Extracting the mixture by decoction over 10 hours in a shaker; In the method of producing a plant disease, pest control agent using a herbal extract comprising the step of adding the ganban stone powder to the extract, stirring and then precipitated and filtered. 상기 한약재는 천문동, 빈랑자, 백부근, 천련자, 정향, 건강, 지유, 사군자, 지모, 대계, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑죽, 포공영, 연교, 금은화, 여로, 건칠피, 용뇌, 연초엽으로 하고, 상기 혼합단계에서 한약재의 농도는 각각 0.003~0.024 중량%인 것을 특징으로 하고, 상기 여과단계에서 맥반석 가루는 0.001~0.01 중량%인 것을 특징으로 하는 한약추출물을 이용한 식물병·해충 방제제의 제조방법.The herbal medicine is astronomical dong, betel nut, baekbugeun, cheonjakja, cloves, health, jiyu, monarchy, jimo, dagyeok, Hakseol, Hwangbaekpi, casualties, wood, gilgyeong, hagocho, love leaf, galleng, black porridge, pogongyoung, yeongyo, geumeunhwa, Yeogi, dried pistil, green onion, soft leaf, the concentration of the herbal medicine in the mixing step is characterized in that 0.003 ~ 0.024% by weight, respectively, the ganban stone powder in the filtration step is characterized in that 0.001 ~ 0.01% by weight Method for producing plant diseases and pest control agents using extracts. 제 1항에 있어서, 상기 한약재의 농도는 천문동, 빈랑자, 백부근, 천련자는 0.0086 중량% 이고, 정향, 건강, 지유, 사군자, 지모, 대계는 0.0057 중량% 이고, 학슬, 황백피, 사상자, 목통, 길경, 하고초, 애엽, 오배자, 흑죽, 포공영, 연교, 금은화, 여로는 0.0029 중량% 이고, 건칠피, 용뇌는 0.0086 중량%, 연초엽은 0.0171 중량% 인 것을 특징으로 하는 한약추출물을 이용한 식물병·해충 방제제의 제조방법.The method of claim 1, wherein the concentration of the medicinal herb is astronomical dong, betel nut, baekbugeun, cheonnyeonul is 0.0086% by weight, clove, health, fat, four-armed, gimo, dagyeok is 0.0057% by weight, Hakseul, Hwangbaekpi, casualties, throat Gilyeong, Hagocho, leafy, fivefold, black porridge, pogongyeong, Yeongyo, gold and silver flower, 0.0029% by weight, dried chile, yongnae 0.0086% by weight, vinegar is 0.0171% by weight plant disease using herbal extracts Preparation of pest control agents. 제 1항의 방법으로 제조된 한약추출물을 이용한 식물병·해충 방제제.Plant disease and pest control using the herbal extract prepared by the method of claim 1.
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KR20010000894A (en) * 2000-10-26 2001-01-05 최인귀 Agricultural medicines consist of herbs
KR20010067650A (en) * 2001-02-28 2001-07-13 김좌환 Korean medicine agriculture a method
KR20010044712A (en) * 2001-03-19 2001-06-05 이수형 Composition for control of plant diseases containing Eugenia caryophyllate

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KR101174042B1 (en) * 2009-04-09 2012-08-16 박성희 Mixture extract having insecticidal and repellent effects and products using the same
CN102696878A (en) * 2011-03-03 2012-10-03 李昌海 Feed additive
WO2014038914A1 (en) * 2012-09-10 2014-03-13 Suh Hwa Soo Agrichemical composition for preventive treatment for anthracnose disease and viral disease
KR101306885B1 (en) 2013-04-24 2013-09-10 남부대학교산학협력단 A composition for control of blast comprising the extract of annamorena asphodeloides as an effective component, microcapsule comprising of the same and a control method of blast using the same

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