KR20040056061A - Control Agent and Manufacturing Process in Powdery Mildew of Plants - Google Patents

Abstract

PURPOSE: provided are a control agent for powdery mildew in plants and a manufacturing process thereof by extracting natural medicinal products and filtering them. The control agent has excellent therapeutic effect on powdery mildew, gives disease resistance to plants and promotes rooting of them. CONSTITUTION: A control agent for powdery mildew is characterized by containing extract or powder of Rhus javanica Linne and antibacterial materials, wherein the extract of Rhus javanica Linne exerts plants to have disease resistance, and promotes rooting of plants. therefore, it increases the growth of plants and their fruits, and is free from toxicity and environmental pollution.

Description

Composition for controlling plant powdery mildew and its manufacturing method {Control Agent and Manufacturing Process in Powdery Mildew of Plants}

The present invention relates to a composition for controlling plant diseases and a method for producing the same, which effectively control powdery mildew occurring in crops by extracting and filtering gallastia.

In agriculture, chemical pesticides are widely used for mass production of crops. These chemical pesticides are used differently depending on the type of crops and pests, and there are now hundreds of species. In recent years, due to the continuous use of chemical pesticides, the problem of resistance to pests has emerged, and toxic pesticides in agricultural products are detected beyond the allowable value, and in many cases, the value as food is lost.

The development of plant pest control agents that are not toxic to humans and have low environmental pollution are the researchers' biggest challenges. As a solution to this problem can be solved through the development of a microbial agent and a product using a natural product, research on this is being actively conducted, and these formulations are being sold, but the usage is very low due to the low control price. to be. The development of eco-friendly products with excellent control effect is still insufficient.

The pathogens of powdery mildew are known as Sphaerotheca fuliginea, Sphaerotheca pannosa, Sphaerotheca humuli, Sphaerotheca phaseoli, Sphaerotheca glycines, Leveillua taurica, and Eryshipe cichoraeearum . Powdery mildew is a plant disease that causes a lot of damage not only in plant cultivation but also in the open field. The onset temperature of powdery mildew is 17-25 degrees and the humidity is 23-99%. In open field cultivation, it usually occurs in May-June and September-November, but in plant cultivation, it occurs all year except in summer heat. The victims of powdery powder are mainly cucumbers, melons, melons, pumpkins, eggplants, peppers, tomatoes, strawberries, peaches, apples, grapes, roses, gerberas, wolfberry, peony, and barley. In powdery mildew, symptoms vary somewhat depending on the crop, but most of the leaves, stems and fruits bloom white powdery mildew, and as the disease progresses, the diseased tissues deform rapidly as the diseased tissues necrosis by inhibiting photosynthesis and respiration. I will die. Therefore, the spread of powder in the leaves causes a great decrease in yield due to the leaf weakening caused by the leaves and leaves, the weakening of the leaves, the growth of the stems and the inability to harvest the fruits. Powdery mildew can be controlled by spraying with chemicals, but pathogens are neat parasitic and form endogenous mycelia, so they spread in the air in the form of conidia. Therefore, periodic control is necessary, and if the control fails, the power of the plant is reduced, the fruit becomes poor, and the yield is greatly reduced.

The gall is an insect worm (worm aphids) of the Rhus chinensis and has an irregular pouch and hollow inside. The chemical components of the gall bladder include gallotannin, resin, starch and tannin. In oriental medicine, it is mainly used for diseases such as astringent, hemostasis, detoxification, diarrhea, gastric ulcer, bloody stool, hematuria, burns and stomatitis. In addition, the magnolia has excellent antibacterial activity against Staphylococcus aureus, Streptococcus, Pneumococcus, Typhoid bacillus, Hepatitis B, and Pseudomonas aeruginosa.

Chemicals for controlling powdery mildew include Myclobutanil, Fenarimol, Thiophanate, Benomyl, Triadimefon, Sulfur, and Chinomethionate. In addition, baking soda, clay, oils and synthetic surfactants are recently used for controlling powdery mildew in crops, and plant extracts and microorganisms, which are not toxic, have been tried.

Therefore, the present invention is to provide a composition for the control of powdery mildew by the extraction and filtration of the active substance from the gall, and to provide an efficient control of powdery mildew and environmentally friendly fungal control agent.

The present invention relates to a herbal medicine and its composition which can be used for the effective control of phytopathogenic powdery mildew disease, which is excellent in controlling the powdery mildew, and obtains the plant resistance of the plant, and does not cause the weakening of crops and has almost no poisoning toxicity. The purpose is to provide an environmentally friendly formulation without.

1: Treatment result of powdered powder control agent using gallastonia extract in pumpkin

2: Treatment result of powdered powder control agent using gall bladder extract in cucumber

3: Treatment result of powder powder control agent using gall bladder extract in strawberry

In order to achieve the above object, the present invention provides a composition and a method for producing powdered malnutrition extract, and the composition using the organol methanol extract and the malnutrition hydrothermal extract effective in powdery mildew control and plant disease resistance.

Hereinafter, the present invention will be described in more detail.

The gall pollen used in the plant powdery mildew and the disease-resistant composition of the present invention is a material readily obtainable from the present herbal medicine, and relates to powdery mildew control agent made of each extract obtained by extraction with methanol and water, or powder thereof. Detailed description of this composition is as follows.

In the present invention, the fungus used for measuring the antimicrobial activity of fungi causing powdery mildew was used separately from the leaves and fruits of strawberries and cucumbers with severe powdery mildew.

The present invention will be described in more detail with reference to the following examples. However, these Examples are only illustrative of the present invention and the scope of the present invention is not limited thereto.

Example 1 Preparation and Validation of Water Extract of Mt.

After crushing using a grinder, 2L water was added to 1 kg of the powder, and the mixture was left at room temperature for 12-24 hours, followed by centrifugation of the extract to collect the supernatant, followed by concentration.

The results of the germination test of the gall bladder water extract are shown in Table 1. The radish seeds were soaked in water, placed on top of the gauze of the culture vessel and germinated in a thermostat. The control group was treated with physiological saline, and each test solution was treated with 10 ml of the gauze of the culture vessel. The germination inhibitory effect was weakly observed in the stock solution and dilution of 1/10, but it can be seen that there is no significant effect on the germination of radish seeds at dilution concentrations above 1/100.

Radish Seed Germination Test of Mellitus Water Extract extract Dilution concentration Stock solution 1/10 1/100 1/200 Water extract ++ + - -

-Normal germination; +, Weak germination; ++, excellent germination inhibition

The results of the weakening test of the gall bladder water extract are shown in Table 2. The extract was diluted by concentration in young cucumber and strawberry hair growth and treated 50 ml. The extract was observed for 10 days after the single treatment compared with the untreated group. The gall bladder water extract was found to have no significant effect on the growth and rooting of cucumbers and strawberries. In the case of treatment of gall bladder water extract it can be seen that it is effective in promoting rooting compared to the control.

Weakness test of gallastula water extract extract Crop and Dilution Concentration cucumber Strawberry 1/100 1/200 1/400 1/100 1/200 1/400 Water extract - - - - - -

-, Similar to untreated and grown

Strawberries and cucumbers seriously affected by powdery mildew were selected from farms and sprayed with gallant extract on infected leaves to observe the healing effect of powdery mildew. The gall water extract inhibited the spore proliferation of powdery mildew in strawberry and cucumber, and also killed effect.

Example 2 Preparation and Validation of Methanol Extract of Mellitus

Extraction was carried out using methanol to increase the yield for the active material of the gall bladder. The extraction method of the gall is extracted with 80% methanol solution which is the most widely used organic solvent extraction method. That is, the gallant was pulverized using a grinder, and then 8L of 80% methanol was added to 1 kg of the powder, and the mixture was left at room temperature for 12-24 hours. The extract was centrifuged to collect the supernatant, and then dried using a concentrator. The dried sample was dissolved in 1 L of water to carry out the following experiment.

The germination test of the gall bladder methanol extract is shown in Table 3. The radish seeds were soaked in water, removed, placed on top of the gauze of the culture vessel and germinated in a thermostat. The control group was treated with physiological saline, and the test solution was treated with 10ml each in the gauze of the culture vessel. Inhibition of germination was weakly observed in the stock solution and dilution of 1/10. In the dilution concentration of 1/100, the germination of radish seeds was slightly inhibited, but there was no significant effect in the dilution concentration of 1/200.

Radish Seed Germination Test of Mellitus Water Extract extract Dilution concentration Stock solution 1/10 1/100 1/200 Water extract ++ ++ + -

-Normal germination; +, Weak germination; ++, excellent germination inhibition

Table 3 shows the results of the weakening test of the five-fold methanol extract. The extract was diluted by concentration in young cucumber and strawberry hair growth and treated 50 ml. The extract was observed for 10 days after the single treatment compared with the untreated group. Methanol methanol extract showed no significant effect on the overall growth and rooting of cucumbers and strawberries. Rather, when treated with a five-fold methanol extract it can be seen that there is a rooting effect compared to the control. Methanol extract was diluted to 1/100 of the treatment group showed only a slight hardening of the leaves of berries and cucumbers, no peculiar crops were observed.

Antitoxic Test of Methanol Extract extract Crop and Dilution Concentration cucumber Strawberry 1/100 1/200 1/400 1/100 1/200 1/400 80% Methanol Extract + - - + - -

-Growth similar to untreated group; +, Sclerosis of leaves compared to untreated

Strawberries and cucumbers severely affected by powdery mildew were selected from farms and sprayed with Methanol methanol extract on infected leaves to observe the healing effect of powdery mildew. Methanol methanol extract was found to be excellent in inhibiting spore killing and growth of powdery mildew in both crops of strawberry and cucumber. Among the crops of strawberry and cucumber, cucumber had better control effect than strawberry. In case of cucumber, the killing effect of spores was observed from 1 day after spraying.

Example 3: Preparation and efficacy test

In order to increase the yield of the active substance, the extraction method of the gall bladder was extracted with hot water. In other words, the gallant was pulverized using a grinder, and 2L of water was added to 1 kg of the powder, followed by warm extraction for 2 hours. The extract was centrifuged to extract only the supernatant. The dried sample was dissolved in 1 L of water to carry out the following experiment.

The germination test of the Rhizoblast hot water extract is shown in Table 5. The radish seeds were soaked in water, removed, placed on top of the gauze of the culture vessel and germinated in a thermostat. The control group was treated with physiological saline, and the test solution was treated with 10ml each in the gauze of the culture vessel. The germination inhibitory effect was weakly observed in the stock solution and 1/10 diluent, and there was no significant effect on the germination of radish seeds at dilution concentrations above 1/100.

Radish Seed Germination Test of Mellitus Hot Water Extract extract Dilution concentration Stock solution 1/10 1/100 1/200 Hydrothermal extract ++ + - -

-Normal germination; +, Weak germination; ++, excellent germination inhibition

Table 6 shows the results of the weakening test of the Rhizoll hot water extract. The extract was diluted by concentration in young cucumber and strawberry hair growth and treated 50 ml. The extract was observed for 10 days after the single treatment compared with the untreated group. The gall bladder hot water extract was found to have little effect on the overall growth and rooting of cucumbers and strawberries. Rather, when treated with the gall hydrothermal extract it can be seen that the rooting and growth promoting effect is superior to the control.

Antitoxic Test of Gall bladder Hot Water Extract extract Crop and Dilution Concentration cucumber Strawberry 1/100 1/200 1/400 1/100 1/200 1/400 Hydrothermal extract + - - + - -

-Growth similar to untreated group; +, Sclerosis of leaves compared to untreated

Strawberries and cucumbers severely affected by powdery mildew were selected from farms and sprayed with foliar hot water extracts on the leaves to observe the healing effect of powdery mildew. Hot water extract was found to be excellent in inhibiting the spore growth of powdery mildew of cucumber and strawberry.

Empirical test on the control effect of powdery mildew on cucumber and strawberry farms in Chungcheongnam-do, Seoul, Korea, was carried out using five-gallon hydrothermal extract. The results are shown in Table 7. As a result of diluting 200 times the hot-water extracts of Cucumber and Cucumber on cucumber and strawberries, it was confirmed that the powdery mildew control effect was excellent in these crops, and that the control effect was higher in cucumber than strawberry.

Control Effects of Crop Waters of Ginseng Root Extract Treatment group Control rate (%) cucumber Strawberry 2 days after foliar spray 10 days after foliar spray 2 days after foliar spray 10 days after foliar spray Control 0 0 0 0 Hydrothermal extract 65 70 50 65

This study was conducted to investigate the control effect of powdery mildew on farmers in Chungju, Chungnam, Yeongam, Chungbuk, Cheongwon, Korea.

The results of observing the degree of control of the five-component hot-water extract on crops seriously affected by powdery mildew are as shown in FIGS. 1, 2, and 3. Fivefold hot water extract was diluted 600-fold to irrigate pumpkin, cucumber and strawberry severely affected by powdery mildew. At the same time, 200-fold diluted extract was observed for 10 days after foliar spraying on crops. In the case of pumpkin and cucumber, the effect was observed from 1 day after the treatment of hot water extract, and the fruit of strawberry powder was proved to be effective in 2-3 days.

Therefore, when gallja extract is treated in parallel with watering and foliar spraying, it can be seen that the effect is excellent in controlling powdery mildew and increasing the disease resistance of crops.

Example 4 Mixed Product Manufacturing Process

The gall bladder powder used in the composition of the irrigation liquid was used by heating extraction with water. Specifically, the extract of Hungaeng hot water extracted was used as a single composition, or in addition, it was prepared by using an excellent antibacterial effect, or a mixture of 50% of its active ingredient and 50% of Hungja hot water extract. Extracts mixed with gall bladder and other herbal medicines may enhance the control and growth promoting effects depending on the degree of powdery mildew and growth condition of crops.

The composition used for the watering wetting agent is powdered with a grinder and then filtered through a strainer. The filtered fine powder can be mixed and used in various ratios according to crops and application methods. Specifically, the gall bladder powder may be used alone, or may be used by mixing 50% soybeans and 50% gallola.

In view of the control effect of powdery mildew and the results of crop growth, the optimal condition for the production of product is to use the gallnut hot water extract.

In addition, by using the gall extract and its powder as the main raw material can be mixed with the herbal and ingredients excellent in the antibacterial effect, the mixing ratio can be produced differently depending on the crop and soil.

In the extraction solvent of the gall bladder, the active substance is separated even when extracted using an organic solvent such as ethanol, butanol, acetone, ether, chloroform, nucleic acid, in addition to water and methanol. It is not limited to and methanol.

In addition, a method of directly spraying gallant's powder formulation may be used for use before the crop is settled.

The extraction method and the extraction solvent may vary depending on the crop characteristics and the addition conditions in the preparation, but are not limited to the above.

The present invention is not limited to the manufacturing process shown in the examples, and may be manufactured by other methods.

As described above, in the present invention, by using the gallenga water or methanol extract to obtain the extract to prepare the foliar spraying and irrigation preparation, it is excellent in the control and healing effect of powdery mildew of crops, to promote the growth of the crop, rooting, disease resistance Improved results can be obtained. The optimum condition in the manufacturing process and application method is that the effect is maximized when manufactured using hydrothermally extracted materials.

Claims (3)

Powdery mildew control of plants containing the active material with water, hot water, methanol extract, powder and antibacterial activity The plant disease control agent according to claim 1, which shows the disease resistance and rooting promoting effect of crops using a single medicinal herb extract The plant disease control agent for irrigation and foliar spraying according to claim 1 or 2, which can be mixed with a single gall bladder extract or an antibacterial herbal medicine and its active ingredient.