CN108272874A - Chinese medicine preparation and preparation method thereof for laying hen - Google Patents
Chinese medicine preparation and preparation method thereof for laying hen Download PDFInfo
- Publication number
- CN108272874A CN108272874A CN201810329319.7A CN201810329319A CN108272874A CN 108272874 A CN108272874 A CN 108272874A CN 201810329319 A CN201810329319 A CN 201810329319A CN 108272874 A CN108272874 A CN 108272874A
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- laying hen
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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- A61K2236/30—Extraction of the material
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Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- Pharmacology & Pharmacy (AREA)
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- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Neurosurgery (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to pharmaceutic preparation for livestock technical fields, and in particular to be used for the Chinese medicine preparation and preparation method thereof of laying hen.The Chinese medicine preparation is the innovation to traditional Chinese medicine, is prepared by following parts by weight Chinese medicine material：10~25 parts of radix scutellariae, 10~25 parts of honeysuckle, 15~30 parts of folium isatidis, 5~15 parts of Fructus Forsythiae, 10~25 parts of Radix Astragali, 10~25 parts of radix bupleuri, 5~15 parts of rheum officinale, 5~15 parts of Rhizoma Chuanxiong, 5~15 parts of motherwort.Above-mentioned composition is using crushing, and ultrasonic extraction decocts extraction, and the techniques such as chitosan purifying are prepared into oral solution or granule, and simple process is efficient, improves drug bioavailability and medicinal material utilization rate, quality of research specification, meets modern herbal medicine developing direction.
Description
Technical field
The invention belongs to pharmaceutic preparation for livestock technical fields, and in particular to the Chinese medicine preparation for laying hen and its preparation side
Method.
Background technology
Traditional herbal medicine and prescription are the treasures in China, and many tradition herbal medicines and prescription can be effectively prevented and treated
Animal epidemic, while antibiotic usage can be reduced, delay or even prevent antibody-resistant bacterium appearance, as whole society's pay attention to day by day is eaten
Product safety and public health security excavate and arrange traditional herbal medicine prescription, are applied to modern peasants and produce, significant.
It is folk remedy that laying hen health, which dissipates, by radix scutellariae 16g, folium isatidis 20g, Fructus Forsythiae 8g, rheum officinale 24g, Rhizoma Chuanxiong 5g, motherwort 5g
Equal Chinese herbal medicines are made, have the function of it is clearing heat and detoxicating, activate blood circulation and disperse blood clots, freeing lung and relieving asthma and QI invigorating midwifery.It is right through a large amount of clinical verifications
Salpingitis of layer chicken caused by many reasons, peritonitis, respiratory tract and Sudden Death Syndrome etc. have good control effect.
Laying hen health dissipates is embodied in publication in 2002 earliest《New complete practical veterinary drug handbook》(second edition) page 353, prescription is
Traditional traditional Chinese powder medicine, preparation process is simply extensive, and effective ingredient cannot be released effectively, and due to the alimentary canal of chicken compared with
It is short, it is poor to the digestion power of powder, cause Drug bioavailability relatively low, generally requires dosage use, further result in medicine
Object utilization rate is relatively low and the wasting of resources；On the other hand, the group administration regimen of modern large-scale cultivation group includes drinking-water, spray
The modes such as mist, spice, powder are appropriate only for spice, limit its use and popularization.In contrast, granule, oral solution can
Effectively extraction retains effective component of chinese medicine, reduces herbal toxic effect, improves drug utilization efficiency, dosing mode is various, is conducive to
It promotes the use of.
Furthermore current laying hen disease there has been new variation and feature, and previous simple infection is in the majority, in recent years, popular poison
Strain is made a variation, and the mixed infection of different virus increases, and secondary infection increases.
It is of particular importance that the deficiencies of traditional laying hen health powder is difficult to control there is also quality, is unfavorable for the rule of herbal medicine
Generalized and modernization.And oral solution, granule quality are easily controllable, can effectively ensure the stabilization of herbal medicine, uniform, effective, symbol
Close the developing direction of modern herbal medicine.
Invention content
The present invention is directed to the deficiency that original laying hen health dissipates, in conjunction with the new generation feature of laying hen epidemic disease, to former laying hen health dissipate into
Row optimization and innovation, provide the Chinese medicine preparation and preparation method thereof for laying hen.
To achieve the above object, the present invention uses following technical scheme：
The present invention is provided to the Chinese medicine preparations of laying hen, are prepared by following parts by weight Chinese medicine material：10~25 parts of radix scutellariae,
10~25 parts of honeysuckle, 15~30 parts of folium isatidis, 5~15 parts of Fructus Forsythiae, 10~25 parts of Radix Astragali, 10~25 parts of radix bupleuri, rheum officinale 5~15
Part, 5~15 parts of Rhizoma Chuanxiong, 5~15 parts of motherwort.
Further, it is prepared by following parts by weight Chinese medicine material：16 parts of radix scutellariae, 16 parts of honeysuckle, folium isatidis 20
Part, 8 parts of Fructus Forsythiae, 16 parts of Radix Astragali, 16 parts of radix bupleuri, 8 parts of rheum officinale, 10 parts of Rhizoma Chuanxiong, 10 parts of motherwort.
Honeysuckle in side, folium isatidis is clearing heat and detoxicating, is monarch drug in a prescription；It is clearing heat and detoxicating to be aided with Fructus Forsythiae, dispersing swelling and dissipating binds, radix scutellariae heat-clearing
Eliminating dampness, purging intense heat and detonicating, the material poison of rheum officinale clearing stomach intestines can enhance the clearing heat and detoxicating effect of monarch drug in a prescription；The Rhizoma Chuanxiong promoting flow of qi and blood circulation, motherwort
Promoting blood circulation dredges stagnant gas in blood, matches Radix Astragali tonifying middle-Jiao and Qi, and radix bupleuri, which rises, lifts yang-energy, and reducing fever by reconciliation is adjuvant.All medicines share, altogether
Play it is clearing heat and detoxicating, it is promoting blood circulation and removing blood stasis, freeing lung and relieving asthma, the work(of QI invigorating midwifery.
Modern pharmacology effect and antiviral, antibacterial, antipyretic anti-inflammatory, improvement blood of the present invention for the Chinese medicine preparation of laying hen
Cycle, reparation damage and raising animal body immune function etc. are related.
Recent researches find that salpingitis, peritonitis, the epidemic diseases such as respiratory tract disease occur for laying hen, mostly with virus, bacterium sense
Situations such as contaminating (mixing is secondary) correlation, and being often accompanied by not high herd immunity, diarrhea and fever, belongs to heat toxin described in Chinese veterinarian
Disease, Etiological and symptom and nineteen ninety generation (contain good etc., poultry disease prevention of wearing, Chinese agriculture Science Press, 1992
Year) compared to being varied from.Chinese medicine preparation addition honeysuckle of the present invention for laying hen strengthens heat-clearing toxin-expelling functions, improves anti-
Bacterium resisting virus, eliminating inflammation acts on；Radix Astragali, radix bupleuri tonifying Qi act sun are added, reducing fever by reconciliation improves laying hen immunity of organisms, brings down a fever；Reduce
Rheum officinale dosage, reduction are captured, and avoid aggravating diarrhea；Increase Rhizoma Chuanxiong, motherwort dosage, enhance promoting flow of qi and blood circulation dissolving stasis effect, improves
Improve blood circulation and repairs lesion capability.
The present invention also provides the preparation methods of the Chinese medicine preparation for laying hen, include the following steps：
Step 1：Coarse crushing：Take the Chinese medicine material radix scutellariae of above-mentioned parts by weight, honeysuckle, folium isatidis, Fructus Forsythiae, Radix Astragali, radix bupleuri, big
Huang, Rhizoma Chuanxiong, motherwort crush, mixing, cross 40 mesh sieve, obtain Chinese medicine material mixture；
Step 2：It impregnates, ultrasonic extraction：Add the water of 4~6 times of Chinese medicine material mixture weights into Chinese medicine material mixture, then
It is placed in ultrasonic Chinese medicine extraction machine, 1~2h is impregnated under the conditions of 20~40 DEG C；Then 30~60min of ultrasonic extraction must be extracted
Mixture；
Step 3：It decocts：Step 2 gained extraction mixture is transferred to Chinese medicine extracting tank, after being boiled by fire, it is cooked by slow fire 40~
60min, filtering, collection first time filtrate is spare and isolates the dregs of a decoction；Then the water of 3~5 times of dregs of a decoction weight is added into the dregs of a decoction,
After being boiled by fire, it is cooked by slow fire 30~40min, is filtered, second of filtrate is collected；Merge first time filtrate and second of filtrate,
Negative pressure leaching obtains supernatant；
Step 4：Concentration：Supernatant is concentrated under reduced pressure, concentrate a, the volume matter of the concentrate a and Chinese medicine material mixture are obtained
Amount is than being 1: 0.75；
Step 5：Purifying：The pH value for adjusting concentrate a is 4~6, is heated to 40~50 DEG C, is then added in every 1ml concentrates a
1mL chitosan clarifiers, it is stirring while adding, obtain mixed liquor；Mixed solution is at the uniform velocity stirred into 0.5h, is placed for 24 hours, negative pressure leaching,
Collect filtrate；
Step 6：Gained filtrate decompression is concentrated, concentrate b or medicinal extract are obtained, by concentrate b or medicinal extract respectively with it is pharmaceutically acceptable
Auxiliary material mixing, be prepared into oral solution or granule.
Further, the volume mass ratio of the concentrate b and Chinese medicine material mixture is 1: 1, and concentrate b is filling,
Sterilizing, is prepared into oral solution.
Further, the relative density of the medicinal extract is 1.30~1.35, and sucrose, dextrin and volume point are added into medicinal extract
The ethyl alcohol of number 70%, is prepared into granule.
Further, the temperature of the medicinal extract is 50 DEG C.
Further, above-mentioned chitosan clarifier includes the chitosan of 1%m/v and the glacial acetic acid of 0.6%v/v.
Compared with prior art, the beneficial effects of the present invention are：
1, the present invention strengthens heat-clearing toxin-expelling functions for addition honeysuckle in the Chinese medicine preparation of laying hen, improves anti-bacteria and anti-virus
Antiinflammation；Radix Astragali, radix bupleuri tonifying Qi act sun are added, reducing fever by reconciliation improves laying hen immunity of organisms, brings down a fever；Reduce rheum officinale dosage,
Reduction is captured, and avoids aggravating diarrhea；Increase Rhizoma Chuanxiong, motherwort dosage, enhance promoting flow of qi and blood circulation dissolving stasis effect, improving, which improves blood, follows
Ring and reparation lesion capability.
2, the present invention more meets the laying hen reality cause of disease and symptom, on probation in more farms, to egg caused by many reasons
Hen salpingitis, peritonitis, respiratory tract and Sudden Death Syndrome etc. are safe and effective, and up to 88% or more, control effect is better than treated effect
Traditional powder.
3, the present invention is prepared as granule, oral solution through modern crafts, is conducive to the modernization of Chinese medicine, standardization；It is easy to use,
Bioavilability is high, improves medicinal material utilization rate, economizes on resources.
4, the present invention is pure Chinese medicinal preparation, and green noresidue can reduce antibiotic usage, effectively safeguard food security.
Specific implementation mode
Following embodiment is not used to limit protection scope of the present invention for illustrating the present invention.Unless otherwise specified, real
Apply the conventional means that technological means used in example is well known to those skilled in the art.
Embodiment 1
For the Chinese medicine preparation of laying hen, it is prepared by following parts by weight Chinese medicine material：It is 10 parts of radix scutellariae, 10 parts of honeysuckle, big
15 parts of leafiness, 5 parts of Fructus Forsythiae, 10 parts of Radix Astragali, 10 parts of radix bupleuri, 5 parts of rheum officinale, 5 parts of Rhizoma Chuanxiong, 5 parts of motherwort.
Embodiment 2
For the Chinese medicine preparation of laying hen, it is prepared by following parts by weight Chinese medicine material：It is 25 parts of radix scutellariae, 25 parts of honeysuckle, big
30 parts of leafiness, 15 parts of Fructus Forsythiae, 25 parts of Radix Astragali, 25 parts of radix bupleuri, 15 parts of rheum officinale, 15 parts of Rhizoma Chuanxiong, 15 parts of motherwort.
Embodiment 3
For the Chinese medicine preparation of laying hen, it is prepared by following parts by weight Chinese medicine material：It is 13 parts of radix scutellariae, 13 parts of honeysuckle, big
18 parts of leafiness, 6 parts of Fructus Forsythiae, 13 parts of Radix Astragali, 13 parts of radix bupleuri, 6 parts of rheum officinale, 8 parts of Rhizoma Chuanxiong, 8 parts of motherwort.
Embodiment 4
For the Chinese medicine preparation of laying hen, it is prepared by following parts by weight Chinese medicine material：It is 20 parts of radix scutellariae, 20 parts of honeysuckle, big
25 parts of leafiness, 13 parts of Fructus Forsythiae, 20 parts of Radix Astragali, 20 parts of radix bupleuri, 13 parts of rheum officinale, 14 parts of Rhizoma Chuanxiong, 14 parts of motherwort.
Embodiment 5
For the Chinese medicine preparation of laying hen, it is prepared by following parts by weight Chinese medicine material：It is 16 parts of radix scutellariae, 16 parts of honeysuckle, big
20 parts of leafiness, 8 parts of Fructus Forsythiae, 16 parts of Radix Astragali, 16 parts of radix bupleuri, 8 parts of rheum officinale, 10 parts of Rhizoma Chuanxiong, 10 parts of motherwort.
The method that embodiment 6 prepares oral solution
Step 1：Coarse crushing：Take 16 parts of Chinese medicine material radix scutellariae, 16 parts of honeysuckle, 20 parts of folium isatidis, 8 parts of Fructus Forsythiae, 16 parts of Radix Astragali, bavin
16 parts recklessly, 8 parts of rheum officinale, 10 parts of Rhizoma Chuanxiong, 10 parts of motherwort.It by the Chinese medicine material of above-mentioned parts by weight, crushes, 40 mesh are crossed in mixing
Sieve, it is spare to obtain Chinese medicine material mixture.
Step 2：It impregnates, ultrasonic extraction：Add the water of 4 times of Chinese medicine material mixture weights into Chinese medicine material mixture, so
It is placed in Ymnl-10B type ultrasonic Chinese medicine extraction machines, impregnates 2h under the conditions of 20 DEG C；Then under the conditions of 20KHz, 500W,
Ultrasonic extraction 60min, obtains extraction mixture.
Step 3：It decocts：Step 2 gained extraction mixture is transferred to Chinese medicine extracting tank, after being boiled by fire, is cooked by slow fire
40min, filtering, collection first time filtrate is spare and isolates the dregs of a decoction；Then the water of 3 times of dregs of a decoction weight is added into the dregs of a decoction, greatly
After fire boils, it is cooked by slow fire 30min, is filtered, second of filtrate is collected；Merge first time filtrate and second of filtrate, negative pressure is taken out
Filter to obtain supernatant.
Step 4：Concentration：Supernatant is concentrated under reduced pressure, concentrate a, the body of the concentrate a and Chinese medicine material mixture are obtained
Product mass ratio is 1: 0.75, i.e. 1ml concentrates a is equivalent to 0.75g Chinese medicine material mixtures.
Step 5：Purifying：The pH value for adjusting concentrate a is 4, is heated to 40 DEG C, and 1mL then is added in every 1mL concentrates a
Chitosan clarifier, it is stirring while adding, obtain mixed liquor；Mixed solution is at the uniform velocity stirred into 0.5h, is placed for 24 hours, negative pressure leaching is collected
Filtrate；The chitosan clarifier includes the chitosan of 1%m/v and the glacial acetic acid of 0.6%v/v.
Step 6：Gained filtrate decompression is concentrated, concentrate b, the volume of the concentrate b and Chinese medicine material mixture are obtained
Mass ratio is 1: 1, i.e. 1mL concentrates b is equivalent to 1g Chinese medicine material mixtures, and concentrate b is filling, and sterilizing is prepared into oral
Liquid.
The quality research of 7 oral solution of embodiment
[character] this product is brown liquid；Bitter.
[discriminating] (1) takes this product 1ml, adds 75% ethanol solution 5mL of volume fraction, shakes up, as test solution.Separately take
Scutelloside reference substance and chlorogenic acid reference substance add 75% ethyl alcohol of volume fraction that the solution of concentration 0.1mg/mL is made respectively, as
Reference substance solution.It is tested according to thin-layered chromatography (2015 editions two annex of Republic of China Veterinary Pharmacopoeia), draws above-mentioned three kinds
Each 1~2 μ L of solution put respectively on same polyamide film, using acetic acid as solvent, are unfolded, take out, dry, set ultraviolet lamp
It is inspected under (365nm).In test sample chromatography, on scutelloside reference substance chromatography corresponding position, the spot of same color is shown；
With on chlorogenic acid reference substance chromatography corresponding position, the fluorescence spot of same color is shown.
(2) this product 30mL is taken, add diethyl ether 20mL, and shaking extraction takes ether solution, adds water washing 2 times, each 20mL is discarded
Aqueous, ether low temperature are evaporated, and residue adds ethyl acetate 1mL to make it dissolve, as test solution；Separately take Rhizoma Chuanxiong control medicinal material
0.5g adds diethyl ether and is ultrasonically treated 10min, filtration, and filtrate volatilizes, and residue adds ethyl acetate 1mL to make it dissolve, as a contrast medicinal material
Solution；It is tested according to thin-layered chromatography (2015 editions two annex of Republic of China Veterinary Pharmacopoeia), draws 10 μ L of test solution,
2 μ L of control medicinal material solution are put respectively on same silica gel g thin-layer plate, with n-hexane-ethyl acetate (9:1) it is solvent, exhibition
It opens, takes out, dry, set and inspected under ultraviolet lamp (365nm).In test sample chromatography, in position corresponding with control medicinal material chromatography
On, show the fluorescence spot of same color.
[inspection]
Relative density：1.05 (2015 editions two annex of Chinese veterinary pharmacopoeia) should be not less than.
PH value：It should be 4.0~6.0 (2015 editions two annex of Chinese veterinary pharmacopoeia).
Other：Related every regulation (2015 editions two annex of Chinese veterinary pharmacopoeia) under mixture item should be met.
[assay] is measured according to high performance liquid chromatography (2015 editions two annex of Chinese veterinary pharmacopoeia).
Chromatographic condition and system suitability：It is filler with octadecylsilane chemically bonded silica；- 1% acetic acid of methanol
Solution (44:56) it is mobile phase, Detection wavelength 278nm.Number of theoretical plate is calculated by baicalin peak, should be not less than 2000.
The preparation of reference substance solution：Take scutelloside reference substance appropriate, it is accurately weighed, add 50% methanol of percentage by volume to be made
The solution of 25 μ g/mL to get.
The preparation of test solution：Precision weighs this product 1ml, sets 50mL measuring bottles, and 50% methanol of percentage by volume is added to dissolve
And be diluted to 50mL scales, shake up, filter, take subsequent filtrate to get.
Measuring method：It is accurate respectively to measure reference substance solution and each 10 μ L of test solution, liquid chromatograph is injected, is measured,
To obtain the final product.
This product contains radix scutellariae per 1mL based on scutelloside (C21Hl8O11), must not be less than 3.5mg.
The method that embodiment 8 prepares granule for the Chinese medicine composition of laying hen
Step 1：Coarse crushing：Take 16 parts of Chinese medicine material radix scutellariae, 16 parts of honeysuckle, 20 parts of folium isatidis, 8 parts of Fructus Forsythiae, 16 parts of Radix Astragali, bavin
16 parts recklessly, 8 parts of rheum officinale, 10 parts of Rhizoma Chuanxiong, 10 parts of motherwort.It by the Chinese medicine material of above-mentioned parts by weight, crushes, 40 mesh are crossed in mixing
Sieve, it is spare to obtain Chinese medicine material mixture.
Step 2：It impregnates, ultrasonic extraction：Add the water of 6 times of Chinese medicine material mixture weights into Chinese medicine material mixture, so
It is placed in Ymnl-10B type ultrasonic Chinese medicine extraction machines, impregnates 1h under the conditions of 40 DEG C；Then under the conditions of 20KHz, 500W,
Ultrasonic extraction 30min, obtains extraction mixture.
Step 3：It decocts：Step 2 gained extraction mixture is transferred to Chinese medicine extracting tank, after being boiled by fire, is cooked by slow fire
60min, filtering, collection first time filtrate is spare and isolates the dregs of a decoction；Then the water of 5 times of dregs of a decoction weight is added into the dregs of a decoction, greatly
After fire boils, it is cooked by slow fire 40min, is filtered, second of filtrate is collected；Merge first time filtrate and second of filtrate, negative pressure is taken out
Filter to obtain supernatant.
Step 4：Concentration：Supernatant is concentrated under reduced pressure, concentrate a, the body of the concentrate a and Chinese medicine material mixture are obtained
Product mass ratio is 1: 0.75, i.e. 1mL concentrates a is equivalent to 0.75g Chinese medicine material mixtures.
Step 5：Purifying：The pH value for adjusting concentrate a is 6, is heated to 50 DEG C, and 1mL then is added in every 1mL concentrates a
Chitosan clarifier, it is stirring while adding, obtain mixed liquor；Mixed solution at the uniform velocity stirs 0.5h, places for 24 hours, and negative pressure leaching collects filter
Liquid；The chitosan clarifier includes the chitosan of 1%m/v and the glacial acetic acid of 0.6%v/v.
Step 6：Granulation：By the medicinal extract that the concentration of gained filtrate decompression is relative density 1.30 (50 DEG C).In mixing machine, according to
15% medicinal extract, 45% sucrose, 40% dextrin, uniform stirring 15min is added in weight meter；Then 1.5% is added thereto
(v/m) ethyl alcohol that volume fraction is 70%, uniformly mixes 30min, obtains mixture；Mixture is put into squash type granulator
In, soft grain is made, dry, whole grain is packed to get granule.
Embodiment 9
Step 1~5 are same as Example 8 in the present embodiment, the difference is that step 6：Granulation：Gained filtrate decompression is dense
It is condensed to the medicinal extract of relative density 1.35 (50 DEG C).In mixing machine, according to weight meter, it is added 15% medicinal extract, 45% sucrose,
40% dextrin, uniform stirring 45min；Then the ethyl alcohol that 0.8% (v/m) volume fraction is 70% is added thereto, it is uniformly mixed
15min is closed, mixture is obtained；Mixture is put into squash type granulator, soft grain is made, dry, whole grain, packaging to get
Granula.
The quality research of 10 granule of embodiment
[character] this product is the particle of brown color；It is sweet, slight bitter.
[discriminating] (1) takes this product 2g, adds 75% ethanol solution 10mL of volume fraction, and setting heating in water-bath makes dissolving, filters,
Filtrate is as test solution.Scutelloside reference substance and chlorogenic acid reference substance separately are taken, respectively plus 75% ethyl alcohol of volume fraction is made
The solution of 0.1mg/mL, as a contrast liquid.It is tried according to thin-layered chromatography (2015 editions two annex of Republic of China Veterinary Pharmacopoeia)
It tests, draws above-mentioned each 1~2 μ L of three kinds of solution, put respectively on same polyamide film, using acetic acid as solvent, expansion takes
Go out, dry, sets and inspected under ultraviolet lamp (365nm).In test sample chromatography, on scutelloside reference substance chromatography corresponding position, show
The spot of same color；With on chlorogenic acid reference substance chromatography corresponding position, the fluorescence spot of same color is shown.
(2) this product 12g is taken, it is finely ground, add water 100mL to make it dissolve, filters, filtrate is taken to be shaken under the conditions of 90 DEG C with petroleum ether
Extraction 2 times, each 20mL is shaken, merges petroleum ether extract, about 1mL is concentrated into, as test solution.Separately take Rhizoma Chuanxiong comparison medicine
Material 0.5g is made in the same way of control medicinal material solution.According to thin-layered chromatography (2015 editions two annex of Republic of China Veterinary Pharmacopoeia)
Each 10 μ L of above two solution are drawn in experiment, are put respectively on same silica gel g thin-layer plate, with n-hexane-ethyl acetate (9:1)
For solvent, it is unfolded, takes out, dry, set and inspected under ultraviolet lamp (365nm).In test sample chromatography, with control medicinal material chromatography
On corresponding position, a fluorescence principal spot of same color is shown.
[inspection] should meet related every regulation (2015 editions two annex of Chinese veterinary pharmacopoeia) under granule item.
[assay] is measured according to high performance liquid chromatography (2015 editions two annex of Chinese veterinary pharmacopoeia).
Chromatographic condition and system suitability：It is filler with octadecylsilane chemically bonded silica；- 0.4% phosphorus of methanol
Acid solution (50:50) it is mobile phase, Detection wavelength 280nm.Number of theoretical plate is calculated by baicalin peak, should be not less than 2500.
The preparation of reference substance solution：Take scutelloside reference substance appropriate, it is accurately weighed, add 70% ethyl alcohol of volume fraction to be made often
The Solutions Solution of 50ug/mL to get.
The preparation of test solution：This product about 1g is taken, finely ground, volume fraction 70% ethyl alcohol 50mL is added in precision, weighed heavy
Amount is ultrasonically treated (250w, 25KHz) 20min and makes it dissolve, cooling, then weighed weight, is supplied and is subtracted with 70% ethyl alcohol of volume fraction
The weight of mistake, shakes up, filtration, take subsequent filtrate to get.
Measuring method：It is accurate respectively to measure reference substance solution and each 5 μ L of test solution, liquid chromatograph is injected, is measured, i.e.,
.
This product contains radix scutellariae per 10g based on scutelloside (C21Hl8O11), must not be less than 40mg.
11 clinical application of embodiment
Illustrate beneficial effects of the present invention with reference to clinical application：
Salpingitis diagnoses：With respiratory symptom, some laying hen appearances are all gone well laying hen group, and blood is carried on sick birds, beasts and eggs shell
Mark, eggshell quality are deteriorated.Frequent yellow, the white purulence sample secretion of discharge in fallopian tubal pollutes perianal and feather below,
It lays eggs difficulty.Dissect lesion is mainly ovarian follicle denaturation, deformation, hyperemia, bleeding, necrosis or atrophy；It is fallopian tubal oedema, thicker, it is interior
There are a large amount of secretion, peritoneal inflammation, hyperemia, muddiness.There is the serious laying hen ovarian follicle of the state of an illness to fall into abdominal cavity, forms yolk peritonaeum
Inflammation, enteron aisle sticks together with peritonaeum or abdominal cavity enteron aisle, internal organs stick together；Abdominal cavity product has troubled liquor, stench, some laying hen abdomens
Chamber has yellow-white caseous substance.
Application method and dosage：
It is watered 200kg using oral solution 100mL made from embodiment 6, morbidity chicken group concentrates drinking-water 2~3 hours daily, is used in conjunction 7
It.
It is watered 200kg using granule 100g made from embodiment 8, morbidity chicken group concentrates drinking-water 2~3 hours daily, even
With 7 days.
Using granule 100g spice 100kg made from embodiment 9, morbidity chicken group concentrates feeding 2~3 hours daily, even
With 7 days.
Laying hen health dissipates application method and dosage：By 100g spice 100kg, morbidity chicken group concentrates feeding 2~3 hours daily,
It is used in conjunction 7 days.
Efficacy assessment standard：
Recovery from illness：Respiratory symptom disappears after medication, and mental restoration is normal, and eggshell quality is normal.Fallopian tubal secretion without exception, anus
All contamination-frees.Dissect ovarian follicle, fallopian tubal are without macroscopic lesion.
Effectively：Various aspects symptom has clear improvement after medication.
In vain：Various aspects symptom is not improved after animal-use drug.
It is efficient：(recovery from illness number+significant figure)/always treat number.
Medication is grouped to certain breeding layer chicken field morbidity chicken, every group 200, clinical treatment is counted, therapeutic effect
As shown in table 1.
1 therapeutic effect of table
| Drug classification | Application method | Total (only) | Cure number (only) | Significant figure (only) | Invalid number (only) | It is efficient |
| Laying hen health dissipates | Spice | 200 | 120 | 48 | 32 | 84% |
| Oral solution | It is watered | 200 | 140 | 40 | 20 | 90% |
| Granule (embodiment 8) | It is watered | 200 | 126 | 50 | 24 | 88% |
| Granule (embodiment 9) | Spice | 200 | 128 | 50 | 22 | 89% |
Illness laying hen does not occur adverse reaction in therapeutic process, is treated through 7 days, and the present invention cures 88% or more effective percentage,
It is dissipated better than existing laying hen health.
The embodiment of the above is only used to explain the present invention, the scope of the present invention is not limited, for this technology
It, certainly can be according to technology contents disclosed in this specification, by way of replacing or changing for the technical staff in field
Other embodiments are made easily, therefore all changes and improvements etc. done in the principle of the present invention and process conditions, it should all wrap
It includes in scope of the present invention patent.
Claims (7)
1. the Chinese medicine preparation for laying hen, which is characterized in that be prepared by following parts by weight Chinese medicine material：Radix scutellariae 10~25
Part, 10~25 parts of honeysuckle, 15~30 parts of folium isatidis, 5~15 parts of Fructus Forsythiae, 10~25 parts of Radix Astragali, 10~25 parts of radix bupleuri, rheum officinale 5
~15 parts, 5~15 parts of Rhizoma Chuanxiong, 5~15 parts of motherwort.
2. the Chinese medicine preparation according to claim 1 for laying hen, which is characterized in that by following parts by weight Chinese medicine material
It is prepared：16 parts of radix scutellariae, 16 parts of honeysuckle, 20 parts of folium isatidis, 8 parts of Fructus Forsythiae, 16 parts of Radix Astragali, 16 parts of radix bupleuri, 8 parts of rheum officinale, river
10 parts of rhizome of chuanxiong, 10 parts of motherwort.
3. the preparation method based on the Chinese medicine preparation described in claim 1 for laying hen, which is characterized in that including following step
Suddenly：
Step 1：Coarse crushing：Take the Chinese medicine material radix scutellariae of above-mentioned parts by weight, honeysuckle, folium isatidis, Fructus Forsythiae, Radix Astragali, radix bupleuri, big
Yellow, Rhizoma Chuanxiong and motherwort crush, mixing, cross 40 mesh sieve, obtain Chinese medicine material mixture；
Step 2：It impregnates, ultrasonic extraction：Add the water of 4~6 times of Chinese medicine material mixture weights into Chinese medicine material mixture, then
It is placed in ultrasonic Chinese medicine extraction machine, 1~2h is impregnated under the conditions of 20~40 DEG C；Then 30~60min of ultrasonic extraction must be extracted
Mixture；
Step 3：It decocts：Step 2 gained extraction mixture is transferred to Chinese medicine extracting tank, after being boiled by fire, it is cooked by slow fire 40~
60min, filtering, collection first time filtrate is spare and isolates the dregs of a decoction；Then the water of 3~5 times of dregs of a decoction weight is added into the dregs of a decoction,
After being boiled by fire, it is cooked by slow fire 30~40min, is filtered, second of filtrate is collected；Merge first time filtrate and second of filtrate,
Negative pressure leaching obtains supernatant；
Step 4：Concentration：Supernatant is concentrated under reduced pressure, concentrate a, the volume matter of the concentrate a and Chinese medicine material mixture are obtained
Amount is than being 1: 0.75；
Step 5：Purifying：The pH value for adjusting concentrate a is 4~6, is heated to 40~50 DEG C, is then added in every 1ml concentrates a
1mL chitosan clarifiers, it is stirring while adding, obtain mixed liquor；Mixed solution is at the uniform velocity stirred to 0.5 h, places 24 h, negative pressure is taken out
Filtrate is collected in filter；
Step 6：Gained filtrate decompression is concentrated, concentrate b or medicinal extract are obtained, by concentrate b or medicinal extract respectively with it is pharmaceutically acceptable
Auxiliary material mixing, be prepared into oral solution or granule.
4. the preparation method of the Chinese medicine preparation according to claim 3 for laying hen, which is characterized in that the concentrate b
Volume mass ratio with Chinese medicine material mixture is 1: 1, and concentrate b is filling, and sterilizing is prepared into oral solution.
5. the preparation method of the Chinese medicine preparation according to claim 3 for laying hen, which is characterized in that the phase of the medicinal extract
It is 1.30~1.35 to density, the ethyl alcohol of sucrose, dextrin and volume fraction 70% is added into medicinal extract, is prepared into granule.
6. the preparation method of the Chinese medicine preparation according to claim 3 for laying hen, which is characterized in that the temperature of the medicinal extract
Degree is 50 DEG C.
7. the preparation method according to claim 3~6 any one of them for the Chinese medicine preparation of laying hen, which is characterized in that institute
It includes the chitosan of 1%m/v and the glacial acetic acid of 0.6%v/v to state chitosan clarifier.
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Cited By (3)
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| CN109432222A (en) * | 2018-12-18 | 2019-03-08 | 聊城大学 | A kind of Chinese medicine composition for preventing and treating salpingitis of layer chicken |
| CN109528965A (en) * | 2019-01-23 | 2019-03-29 | 河南焱地农牧科技有限公司 | Ensure the novel traditional Chinese medicine for livestock preparation and preparation method thereof of birds genital tract health |
| CN110478405A (en) * | 2019-09-06 | 2019-11-22 | 广东广盈生物技术有限公司 | Nourishing and protecting liver Chinese medicine composition and its preparation and application |
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| CN109432222A (en) * | 2018-12-18 | 2019-03-08 | 聊城大学 | A kind of Chinese medicine composition for preventing and treating salpingitis of layer chicken |
| CN109528965A (en) * | 2019-01-23 | 2019-03-29 | 河南焱地农牧科技有限公司 | Ensure the novel traditional Chinese medicine for livestock preparation and preparation method thereof of birds genital tract health |
| CN110478405A (en) * | 2019-09-06 | 2019-11-22 | 广东广盈生物技术有限公司 | Nourishing and protecting liver Chinese medicine composition and its preparation and application |
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