CN103040924B - Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis - Google Patents

Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis Download PDF

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CN103040924B
CN103040924B CN 201310011345 CN201310011345A CN103040924B CN 103040924 B CN103040924 B CN 103040924B CN 201310011345 CN201310011345 CN 201310011345 CN 201310011345 A CN201310011345 A CN 201310011345A CN 103040924 B CN103040924 B CN 103040924B
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extract
bovine
vigorously
extracts
ethyl acetate
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CN103040924A (en
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韩长日
陈光英
张金超
舒火明
王呈文
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海南师范大学
河北大学
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Abstract

本发明公开了一种牛大力提取物及其在制备治疗骨质疏松药物中的应用。 The present invention discloses a vigorously bovine extract and its use in the preparation of a medicament for the treatment of osteoporosis. 所述牛大力提取物是将牛大力的根烘干并粉碎成粉末,然后用醇回流提取,过滤提取液,减压回收尽溶剂,获得浸膏状牛大力提取物。 The extract is great cattle to bovine roots vigorously dried and pulverized into powder, and then extracted with alcohol at reflux, the extract was filtered under reduced pressure to recover the solvent do, like bovine extract obtained extract vigorously. 在药效试验基础上,发现牛大力提取物在治疗骨质疏松上具有良好的药效,可以用来制备治疗骨质疏松药物,为骨质疏松的治疗提供有效途径,其制备方法简单,成本低。 In efficacy trials, based on extracts of cattle vigorously with good efficacy in the treatment of osteoporosis, may be used for treating osteoporosis drugs, to provide an effective way for the treatment of osteoporosis, their preparation method is simple, cost low.

Description

牛大力提取物及其在制备治疗骨质疏松药物中的应用 Bovine vigorously extract and its use in the preparation of a medicament for the treatment of osteoporosis

技术领域 FIELD

[0001] 本发明属植物应用领域,涉及一种植物活性提取物及其应用,具体是一种牛大力提取物及其在制备治疗骨质疏松药物中的应用。 Plants applications invention relates to a plant extract and its active use, in particular a bovine vigorously extract and its application in osteoporosis drugs for treating [0001] In the present.

背景技术 Background technique

[0002] 骨质疏松(osteoporosis, 0P)是以骨量减少、骨微观结构退化、脆性增加和易发生骨折为特征的一种全身性疾病,多发于绝经后妇女、老人和多种慢性疾病患者。 [0002] osteoporosis (osteoporosis, 0P) is reduced bone mass, bone microstructure degradation, increased brittleness and ease of fracture is a systemic disease characterized by women, the elderly and patients with various chronic diseases in postmenopausal after multiple . 由于骨质疏松致残率较高,极大影响着人们的生活质量,给家庭和社会带来沉重的经济负担。 Due to the high morbidity of osteoporosis, which greatly affects the quality of life, family and society a heavy financial burden. 随着人口结构老龄化,骨质疏松的问题越来越突出。 With the aging population, the problem of osteoporosis has become increasingly prominent.

[0003] 现代医学认为骨质疏松的基本病理机制是在骨代谢过程中骨吸收和骨形成的偶联出现了失衡,导致人体内的钙磷代谢不平衡,骨密度逐渐减少而引发的临床症状。 [0003] Modern medicine coupling mechanism underlying pathology of osteoporosis in bone metabolism bone resorption and bone formation appeared imbalance, leading to the body's imbalance of calcium and phosphorus metabolism, decreased bone mineral density and clinical symptoms caused by . 目前临床上对于骨质疏松的治疗尚无理想的方法。 The present study is no ideal method for the treatment of osteoporosis.

[0004] 中国传统医学中虽无骨质疏松的症名,但究其临床表现、发病机制应归属于“骨痹”、“骨痿”的范畴。 [0004] Although there is no disease in traditional Chinese medicine name of osteoporosis, but their clinical presentation, pathogenesis should be attributed to "Gubi", "bone atrophy" category. 中医理论认为,骨质疏松的病因病机主要是肾亏、脾虚和痰瘀阻脉。 Chinese medicine theory, osteoporosis is primary pathogenesis kidney, spleen and phlegm pulse. 治则则为补肾壮骨、健脾益气和活血通络。 Therapeutic compared Bushenzhuanggu, spleen qi and blood circulation network.

[0005] 牛大力(Millettia specisoa Champ.),别名猪脚笠、金钟根、山莲藕、倒吊金钟、大力薯,为豆科崖豆藤属植物,直立或披散亚灌木,高I〜2米。 [0005] great cattle (Millettia specisoa Champ.), Alias ​​Li pig, Admiralty root, mountain lotus root, hung upside down Queensway, vigorously potato, plants, erect or loose subshrubs high I~2 meters leguminous dielsiana . 根系向下直伸,长I米许。 Root for straight downwardly, I long Michelin. 牛大力以根入药,经检索,目前对牛大力(牛大力的根)的药理研究主要是免疫调节作用,护肝作用,抗炎作用,抗氧化、清除自由基作用,抗肿瘤作用,祛痰、镇咳、平喘作用以及抗凝血作用研究,在治疗骨质疏松药物中的应用尚无报道。 Bovine vigorously root medicine, retrieved, present strong pharmacological studies of bovine (cattle vigorously root) is mainly immunomodulatory effect, hepatoprotective effect, anti-inflammatory action, antioxidant, free radical scavenging effect, antitumor effect, expectorants , cough and asthma as well as research anticoagulant effect, used in the treatment of osteoporosis drugs have not been reported.

发明内容 SUMMARY

[0006] 本发明的目的是提供一种牛大力提取物及其在制备治疗骨质疏松药物中的应用。 [0006] The object of the present invention is to provide a strong bovine extract and its use in the preparation of a medicament for the treatment of osteoporosis.

[0007] 本发明所述的牛大力提取物是将牛大力的根烘干并粉碎成粉末,然后用醇回流提取,过滤提取液,减压回收尽溶剂,获得浸膏状牛大力提取物。 The cattle [0007] The present invention will vigorously vigorously extract is dried and pulverized root of cattle into powder and then extracted with alcohol at reflux, the extract was filtered under reduced pressure to recover the solvent do, like bovine extract obtained extract vigorously.

[0008] 一种牛大力极性部位提取物,是取上述牛大力提取物加水稀释,按顺序用石油醚、氯仿和乙酸乙酯依次萃取,将乙酸乙酯萃取液或萃取后剩余的水溶液减压浓缩至浸膏状,真空干燥得到的粉末即为牛大力乙酸乙酯部位提取物、牛大力水相部位提取物。 [0008] A strongly polar extracts of bovine, bovine dilution is to take the above vigorously extracted with water, washed in sequence with petroleum ether, chloroform and ethyl acetate and extracted sequentially, after extraction with ethyl acetate and extracted liquid or the remaining aqueous solution Save concentrated under pressure to extract shape, and dried in vacuo to give ethyl powder is the strong extracts of bovine, bovine extracts of the aqueous phase strongly.

[0009] 本发明还包括上述牛大力提取物、牛大力乙酸乙酯部位提取物、牛大力水相部位提取物在制备治疗骨质疏松药物中的应用。 [0009] The present invention further includes the above vigorously bovine extract, extracts of bovine vigorously with ethyl acetate, the aqueous phase was vigorously bovine extracts of osteoporosis drugs applied in the preparation of the treatment. 发明人在药效试验基础上,发现上述提取物在治疗骨质疏松上具有良好的药效,可以用来制备治疗骨质疏松药物。 The inventors on the basis of efficacy trials, found that the above extract has good efficacy in the treatment of osteoporosis, it may be used for treating osteoporosis drugs.

[0010] 所述治疗骨质疏松药物是将牛大力提取物与常规辅料配合,采用现有工艺制得的临床上允许的内用制剂。 [0010] The pharmaceutical treatment of osteoporosis is the bovine extract with vigorously with conventional adjuvants, to allow use of the preparation obtained from the prior art clinical.

[0011] 所述常规辅料是溶剂、崩解剂、矫味剂、防腐剂、着色剂、粘合剂、润滑剂、润湿剂、 The [0011] solvent is a conventional excipients, disintegrating agents, flavoring agents, preservatives, coloring agents, binders, lubricants, wetting agents,

增稠剂、增溶剂或基质。 Thickeners, solubilizing agents or matrix.

[0012] 所述内用制剂是散剂、颗粒剂、片剂、胶囊剂、丸剂、口服液、分散片、喷雾剂、静脉注射剂、肌肉注射剂、吸入剂或凝胶剂。 [0012] The formulation is used within powders, granules, tablets, capsules, pills, oral liquid, dispersible tablets, sprays, intravenous injection, intramuscular injection, inhalation or gels.

[0013] 本发明还包括上述提取物与祛风湿药、利水渗湿药、活血化瘀药、补虚药中的一种或一种以上的药物组成复方药剂。 [0013] The present invention further includes the above extract with rheumatism drugs, drug diuresis Shenshi, Traditional medicine, tonics of one or more pharmaceutical combination drug composition.

[0014] 所述祛风湿药为木瓜、秦艽、五加皮、桑寄生、狗脊等。 [0014] The drug is rheumatism papaya, Gentiana Wujiapi, mistletoe, Woodwardia like.

[0015] 所述利水渗湿药为茯苓、泽泻等。 [0015] Wetting agent is a diuresis Poria, Alisma like.

[0016] 所述活血化瘀药为川芎、延胡索、牛膝、骨碎补等。 The [0016] Traditional medicine is Chuanxiong, fumaric, Achyranthes, Drynaria like.

[0017] 所述补虚药是补阳药、补阴药、补气药或补血药。 The [0017] Yang tonics are drugs, drug yin, qi drug or drug blood. 所述补阳药包括淫羊藿、杜仲、续断、鹿角胶、菟丝子、补骨脂、仙茅、肉苁蓉、紫河车、巴戟天、鹿茸、胡桃肉、葫芦巴等;所述补阴药包括麦门冬、黄精、枸杞子、女贞子、旱莲草、龟板、鳖甲、石斛、玉竹、天门冬等;所述补气药包括黄芪、山药、甘草、白术、党参、人参、大枣;所述补血药包括熟地黄、当归、何首乌、白苟、阿月父等。 The Yang agents include Epimedium, Eucommia, Dipsacus, antler, dodder, psoralen, Curculigo, Cistanche, Placenta, Morinda, velvet, walnut meat, fenugreek and the like; yin agents include Ophiopogon, Polygonatum, medlar, Ligustrum, Eclipta prostrata, turtle, turtle, Dendrobium, Polygonatum, aspartic like; qi agents include the astragalus, yam, licorice root, Atractylodes, Codonopsis, ginseng , jujube; Rehmannia agents include the blood, angelica, Polygonum, white Gou a month parent like.

[0018] 本发明所提供的牛大力提取物在治疗骨质疏松上具有良好的药效,可以用来开发治疗骨质疏松药物,避免化学合成药副作用大的缺点,为骨质疏松的治疗提供有效途径,其制备方法简单,成本低。 [0018] The present invention provides bovine strong extract has good efficacy in the treatment of osteoporosis, may be used to develop drugs to treat osteoporosis, to avoid the side effects of large chemical compound classes disadvantages, to provide for the treatment of osteoporosis effective way, its preparation method is simple, low cost.

具体实施方式 Detailed ways

[0019] 下面结合实施例,对本发明的具体实施方式作进一步详细描述。 [0019] Example embodiments in conjunction with the following, specific embodiments of the present invention will be further described in detail. 以下实施例用于说明本发明,但不用来限制本发明的范围。 The following examples serve to illustrate the present invention but are not intended to limit the scope of the present invention. 下列实施例中未注明具体条件的实验方法,通常按照常规条件,或按照制造厂商所建议的条件。 Experimental methods without specific conditions in the examples below, are performed under routine conditions, or in accordance with the conditions recommended by the manufacturer.

[0020] 实施例一 [0020] Example a

[0021] 将牛大力的根烘干并粉碎成粉末,粉末用95%甲醇回流提取3次,合并提取液,过滤,减压回收尽甲醇获得浸膏状甲醇粗提物,甲醇粗提物加水稀释,然后依次用石油醚、氯仿、乙酸乙酯萃取,分别将萃取液减压浓缩至浸膏状,真空干燥得到的粉末分别为牛大力石油醚部位提取物、氯仿部位提取物、乙酸乙酯部位提取物。 [0021] The dried root and bovine vigorously pulverized into powder, the powder was extracted three times with 95% methanol at reflux, the combined extracts were filtered and recovered under reduced pressure to obtain a methanol extract the best shape methanol extracts, aqueous methanol extracts added diluted, and then extracted successively with petroleum ether, chloroform, ethyl acetate, each extract was concentrated under reduced pressure to extract shape, respectively, and dried in vacuo to give powder extract, chloroform extracts of bovine vigorously petroleum ether, ethyl acetate extracts of. 萃取后剩余的水溶液减压浓缩至浸膏状,真空干燥得到的粉末为牛大力水相部位提取物。 After extraction the remaining aqueous solution was concentrated under reduced pressure to extract shape, and dried in vacuo to obtain a powder extract of the aqueous phase portion cattle vigorously.

[0022] 实施例二 [0022] Second Embodiment

[0023] 将牛大力的根烘干并粉碎成粉末,粉末用95%乙醇回流提取4次,合并提取液,过滤,减压回收尽乙醇获得浸膏状乙醇粗提物,乙醇粗提物加水稀释,然后依次用石油醚、氯仿、乙酸乙酯萃取,分别将萃取液减压浓缩至浸膏状,真空干燥得到的粉末分别为牛大力石油醚部位提取物、氯仿部位提取物、乙酸乙酯部位提取物。 [0023] The dried root and bovine vigorously pulverized into powder, the powder extracted four times with 95% ethanol at reflux, the combined extracts were filtered and recovered under reduced pressure to obtain the ethanol extract best shape crude ethanol extract, ethanol was added to crude water diluted, and then extracted successively with petroleum ether, chloroform, ethyl acetate, each extract was concentrated under reduced pressure to extract shape, respectively, and dried in vacuo to give powder extract, chloroform extracts of bovine vigorously petroleum ether, ethyl acetate extracts of. 萃取后剩余的水溶液减压浓缩至浸膏状,真空干燥得到的粉末为牛大力水相部位提取物。 After extraction the remaining aqueous solution was concentrated under reduced pressure to extract shape, and dried in vacuo to obtain a powder extract of the aqueous phase portion cattle vigorously.

[0024] 实施例三 [0024] Example three

[0025] 将牛大力的根烘干并粉碎成粉末,粉末用75%乙醇回流提取6次,合并提取液,过滤,减压回收尽乙醇获得浸膏状甲醇粗提物,乙醇粗提物加水稀释,然后依次用石油醚、氯仿、乙酸乙酯萃取,分别将萃取液减压浓缩至浸膏状,真空干燥得到的粉末分别为牛大力石油醚部位提取物、氯仿部位提取物、乙酸乙酯部位提取物。 [0025] The dried root and bovine vigorously pulverized into powder, the powder extracted 6 times with 75% ethanol at reflux, the combined extracts were filtered and recovered under reduced pressure to obtain the ethanol extract best shape methanol extracts, crude ethanol extract with water diluted, and then extracted successively with petroleum ether, chloroform, ethyl acetate, each extract was concentrated under reduced pressure to extract shape, respectively, and dried in vacuo to give powder extract, chloroform extracts of bovine vigorously petroleum ether, ethyl acetate extracts of. 萃取后剩余的水溶液减压浓缩至浸膏状,真空干燥得到的粉末为牛大力水相部位提取物。 After extraction the remaining aqueous solution was concentrated under reduced pressure to extract shape, and dried in vacuo to obtain a powder extract of the aqueous phase portion cattle vigorously.

[0026]实验: [0026] Experiment:

[0027] 一、体外试验 [0027] I. In vitro testing

[0028] 将实施例获得的提取物(醇粗提物、石油醚部位提取物、氯仿部位提取物、乙酸乙酯部位提取物、水相部位提取物)分别用PBS (磷酸盐缓冲液)配制成10mg/mL储备液,临用前稀释成1000Pg/mL, 100Pg/mL。 [0028] extract (crude extract alcohol, petroleum ether extracts, extracts of chloroform, ethyl acetate extracts of the aqueous phase extracts of) obtained in the Example embodiment were prepared with PBS (phosphate buffered saline) to 10mg / mL stock solution, diluted immediately before use 1000Pg / mL, 100Pg / mL.

[0029] 实验步骤:取原代培养的鼠成骨细胞(OB)以2X 14个/mL接种于96孔板,每孔体积90μί,在CO2培养箱中培养24h。 [0029] Experimental procedure: Take primary cultured rat osteoblasts (OB) to 2X 14 cells / mL were seeded in 96-well plates, each well volume of 90μί, cultured for 24h in a CO2 incubator. 待细胞贴壁后,加入提取物的PBS溶液ΙΟμί,使提取物的终浓度为lOOPg/mL,lOPg/mLL,培养48h后,每孔加入MTT溶液(5 mg/mL) ΙΟμί,继续在培养箱中孵育4h,倾去上清液后,每孔加入DMSO10PL,振荡后用酶标仪于570nm处测定各孔吸光度值。 When the cells adherent, ΙΟμί PBS was added to extract the final concentration of the extract lOOPg / mL, lOPg / mLL, after 48h incubation, MTT solution was added to each well (5 mg / mL) ΙΟμί, continued in the incubator incubated for 4h, the supernatant was decanted, added to each well DMSO10PL, was shaken using a microplate reader at absorbance of each well was measured at 570nm. 同时设定空白组(以同体积的PBS代替提取物),根据下面公式计算细胞存活率(Cell viability)=(提取物组OD值/空白组OD值)X 100%。 While setting the control group (same volume of PBS instead of extract), based on cell viability (Cell viability) = (OD value extract group / OD value of control group) X 100% according to the following equation.

[0030] 所得结果见表I。 [0030] The results obtained are shown in Table I.

[0031] 表I牛大力各极性部位对成骨细胞增殖的影响 [0031] TABLE I Effect of Bovine strongly polar fractions respective osteoblast proliferation

[0032] [0032]

项目_实施例一_实施例二_实施例三_ A program _ _ Example Example Example three two _ _

牛大力浓度细胞存活率(%) 浓度细胞存活率(%) 浓度细胞存活率Cpg/m (pg/ (pg/m (%) Bovine vigorously concentration cell viability (%) concentration of cell viability (%) concentration of cell viability Cpg / m (pg / (pg / m (%)

L) mL) L) L) mL) L)

空白 100.00士2.14 100.00士2.57 100.00±2.26 Blank disabilities 100.00 100.00 2.14 2.57 100.00 ± 2.26 with disabilities

乙酸乙酯部100 138.8±7.2 广100 139.2士7.09™ 100 138.4±6.86” Ethyl wide portion 100 138.8 ± 7.2 100 139.2 disabilities 7.09 ™ 100 138.4 ± 6.86 "

位提取物10 114.1士3.2广* 10 113..^3.16*" 10 112.7±3.5广水相部位提100 121.7±4.4 广100 122.1±4.74*** 100 122.3±4.23*** Extract 10 bits wide 114.1 disabilities 3.2 * 10 113 .. ^ 3.16 * "10 112.7 ± 3.5 wide portion aqueous extract phase 100 121.7 ± 4.4 wide 100 122.1 ± 4.74 *** 100 122.3 ± 4.23 ***

取物 10 104.69±2.52** 10 104.14±2.63 10 103.85土2.47— Extract 10 104.69 ± 2.52 ** 10 104.14 ± 2.63 10 103.85 2.47 Soil

[0033]注:1、与对照组比较 *Ρ〈0.05,林Ρ〈0.01,林* Ρ〈0.001, [0033] Note: 1, compared with control group * Ρ <0.05, Lin Ρ <0.01, Lin * Ρ <0.001,

[0034] 实验结果表明,牛大力的乙酸乙酯部位和水相部位提取物在lOOPg/mL和lOPg/mL浓度条件下,对成骨细胞均具有明显的促增殖作用。 [0034] The experimental results show the great cattle portions of ethyl acetate extracts of the aqueous phase and at lOOPg / mL and lOPg / mL concentrations of osteoblast proliferation have obvious effect.

[0035] 二、牛大力醇粗提物体内药效学实验实施例 [0035] Second, the crude alcohol bovine vigorously object mentioned Experimental Example pharmacodynamics

[0036] 1.实验方法: [0036] 1. Experimental Method:

[0037] 1.1剂量设计与分组:三个实验组的剂量分别为800、600、200mg/kgBW ;另设去势模型对照、雌二醇阳性对照及假手术阴性对照组。 [0037] 1.1 Design and dose group: dose three experimental groups were 800,600,200mg / kgBW; separate model control ovariectomized, estradiol positive control and a negative control sham group.

[0038] 1.2卵巢摘除术: [0038] 1.2 ovariectomized:

[0039] 首先经大鼠腹腔注射50mg/kgBW戊巴比妥钠溶液麻醉,中下腹部去毛用碘酒感和酒精进行局部消毒后,沿腹壁正中线做Icm左右切口对大鼠实施去势手术,摘除双侧卵巢。 [0039] First, by intraperitoneal injection of 50mg / kgBW sodium pentobarbital anesthesia, the hair of the abdomen to sense local disinfection with iodine and alcohol, the abdominal wall along the midline incision made approximately Icm embodiment ovariectomized rats surgery, removal of both ovaries. 另取一组大鼠手术同上但不摘除双侧卵巢,作假手术对照。 Another group of rats, but not above the surgical removal of both ovaries, false operation control.

[0040] 1.3动物分组: [0040] 1.3 Animal grouping:

[0041] 将手术动物根据体重随机分为三个实验组和两个对照组,即高剂量组、中剂量组、低剂量组、去势模型对照、雌二醇阳性对照,另设假手术阴性对照,每组10只动物单笼饲养。 [0041] The surgical animals were randomly divided into three experimental groups and two control groups, i.e., high dose group, middle dose group, low dose group, model control ovariectomized, estradiol positive control, negative Sham-operated control, 10 animals each single cages. 三个实验组按设计量以ImVlOOgBW经口灌胃给予受试物,假手术阴性对照及去势模型对照以等量去离子水灌胃;雌二醇阳性对照给予剂量为2 mg/kgBW。 Three experimental groups according to the design amount ImVlOOgBW test substance administered by oral gavage, the negative control sham and ovariectomized model group fed an equivalent amount of deionized water; positive control estradiol administered at a dose of 2 mg / kgBW. 全部实验动物均饮用去离子水,每周称重一次,实验周期为12周。 All animals were drinking deionized water, weighed once a week experimental period of 12 weeks.

[0042] 1.4 测定: [0042] 1.4 Determination:

[0043] 动物喂养12周后断头处死,用SD-1000骨密度仪,测量大鼠股骨中点及股骨远心端的骨密度。 [0043] Animals were sacrificed after 12 weeks of feeding, with SD-1000 bone densitometer, measuring the femur and a midpoint of the distal end of the femur bone.

[0044] 2.牛大力醇粗提物对大鼠骨密度的影响: [0044] 2. Bovine strong alcohol crude on bone density in rats:

[0045] 实验结果表明,与模型对照组比较,牛大力醇粗提物中、高剂量组均显著提高大鼠股骨远心端骨密度值和骨中点骨密度值(P < 0.05)。 [0045] The experimental results show that, compared with the model control group, the crude alcohol was vigorously cattle, high dose group were significantly increased femur BMD values ​​distal end and midpoint of the bone mineral density value (P <0.05) mention.

[0046] 以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明技术原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。 [0046] The above are only preferred embodiments of the present invention, it should be noted that those of ordinary skill in the art, in the art without departing from the principles of the present invention is provided, further improvements and modifications may be made to these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (4)

1.一种牛大力提取物、牛大力乙酸乙酯部位提取物、牛大力水相部位提取物在制备治疗骨质疏松药物中的应用;所述牛大力提取物是将牛大力的根烘干并粉碎成粉末,然后用醇回流提取,过滤提取液,减压回收尽溶剂,获得浸膏状牛大力提取物;所述牛大力乙酸乙酯部位提取物是将上述牛大力提取物加水稀释,按顺序用石油醚、氯仿和乙酸乙酯依次萃取,将乙酸乙酯萃取液减压浓缩至浸膏状,真空干燥得到的粉末即为牛大力乙酸乙酯部位提取物;所述牛大力水相部位提取物是将上述牛大力提取物加水稀释,按顺序用石油醚、氯仿和乙酸乙酯依次萃取,萃取后剩余的水溶液减压浓缩至浸膏状,真空干燥得到的粉末即为牛大力水相部位提取物。 A great cattle extracts, ethyl acetate extracts of bovine vigorously, application in the preparation of a medicament for the treatment of osteoporosis bovine strong aqueous phase extraction site; the extract is great cattle Bovine vigorously root drying and pulverized into powder, and then extracted with alcohol at reflux, the extract was filtered under reduced pressure to recover the solvent do, like bovine extract obtained extract vigorously; the ethyl acetate extracts of bovine vigorously above is vigorously bovine extract diluted with water, sequentially extracted sequentially with ethyl acetate and petroleum ether, chloroform, ethyl acetate extract was concentrated under reduced pressure to extract form, a powder that is dried in vacuo to give the ethyl acetate extracts of bovine vigorously; the aqueous phase strongly bovine extracts of bovine above is vigorously extract diluted with water, washed in sequence with petroleum ether, chloroform and ethyl acetate and extracted sequentially, after extraction to extract the remaining aqueous solution was concentrated under reduced pressure to shape, and dried in vacuo to give powder is the strong water cattle extracts of phase.
2.根据权利要求1所述的应用,其特征在于:所述治疗骨质疏松药物是将牛大力提取物与常规辅料配合,采用现有工艺制得的临床上允许的内用制剂。 The use according to claim 1, wherein: said medicament is for treating osteoporosis extract with bovine vigorously with conventional adjuvants, to allow use of the preparation obtained from the prior art clinical.
3.根据权利要求2所述的应用,其特征在于:所述常规辅料是溶剂、崩解剂、矫味剂、防腐剂、着色剂、粘合剂、润滑剂、润湿剂、增稠剂、增溶剂或基质。 3. The use according to claim 2, wherein: said solvent is a conventional excipients, disintegrating agents, flavoring agents, preservatives, coloring agents, binders, lubricants, wetting agents, thickeners , solubilizers or matrix.
4.根据权利要求2所述的应用,其特征在于:所述内用制剂是散剂、颗粒剂、片剂、胶囊剂、丸剂、口服液、喷雾剂、静脉注射剂、肌肉注射剂、吸入剂或凝胶剂。 The use according to claim 2, wherein: said formulation is used within powders, granules, tablets, capsules, pills, oral liquid, spray, intravenous, intramuscular, inhalation or condensate glue.
CN 201310011345 2013-01-14 2013-01-14 Radix millettiae speciosae extract and application thereof in preparing drugs for treating osteoporosis CN103040924B (en)

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